Composition with soothing effect and preparation and application thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to a composition with a soothing effect and preparation and application thereof. The composition comprises cnidium monnieri fruit extract, rosemary leaf oil and an organic solvent, wherein the organic solvent comprises polyol or an oily solvent. The composition disclosed by the invention is stable and clear, can act on multiple targets, and comprehensively and quickly solves the problems of scorching hot and stabbing pain of sensitive skin.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to a composition with soothing effect and a preparation method and application thereof. Background Art

[0002] Sensitive skin is a metastable skin condition. Its main manifestation is that when it is physically or chemically stimulated, the skin is prone to discomfort such as burning, stinging, itching and tightness. Sensitive skin is also called intolerant or easily irritated skin. It often occurs on the face. It is a high-reaction symptom with a long recovery period and easy recurrence. Its causes are complex and the formation mechanism is still unclear. Sensitive skin has a high incidence rate in all countries in the world and has become one of the common skin problems.

[0003] As domestic consumers' demand and spending power for sensitive skin care continue to grow, China, as a key market for sensitive skin care, has a large room for growth. According to the "2021 Quantitative Research Report on Sensitive People (1,200 Samples)" from Miaosen's Consumer Research Center, burning feedback accounted for 35% and stinging feedback accounted for 44%. Therefore, skin burning and stinging problems are problems that nearly half of people with sensitive skin need to solve urgently.

[0004] The occurrence of burning and tingling in sensitive skin involves multiple targets: TRPV1 and TRPA1. TRPV1, as an integrator of various stimuli, is a key core target for burning and tingling. TRPA1 and TRPV1 are co-expressed. Because TRPA1 is sensitive to Ca 2+ sensitive, so TRPA1 acts as a signal amplifier, leading to Ca 2+ The inflow increased further.

[0005] At present, in order to solve the problem of skin burning and stinging, the products in the cosmetics industry mainly focus on inhibiting TRPV1 activation and expression, such as trans-4-tert-butyl cyclohexanol, which is a specific TRPV1 inhibitor that can block the activation of TRPV1 channels induced by capsaicin and reduce the burning sensation caused by capsaicin in the body. However, its target is single and cannot comprehensively and quickly solve the problem of skin burning and stinging, and the symptoms are prone to recurrence. For another example, Cnidium monnieri extract can block the activation of TRPV1 channels, but it also cannot comprehensively and quickly solve the problem of skin burning and stinging, and it is difficult to form a stable liquid phase system with multiple ingredients. Its application in cosmetics is limited, and the effect on the target needs to be further improved. Summary of the invention

[0006] One object of the present invention is to provide a composition that can effectively solve the problems of skin burning and stinging at multiple targets, comprising: cnidium monnieri extract, rosemary leaf oil and an organic solvent; wherein the organic solvent comprises a polyol or an oily solvent.

[0007] In a specific embodiment of the present invention, the polyol includes at least one of dipropylene glycol, propylene glycol, butylene glycol and glycerol.

[0008] In a specific embodiment of the present invention, the polyol includes a first polyol and a second polyol; the first polyol includes at least one of propylene glycol, butylene glycol, glycerol and dipropylene glycol, and the second polyol includes dipropylene glycol.

[0009] In one embodiment of the present invention, the polyol comprises dipropylene glycol and butylene glycol.

[0010] In one embodiment of the present invention, the polyol comprises dipropylene glycol.

[0011] In a specific embodiment of the present invention, the oily solvent comprises caprylic / capric triglyceride.

[0012] In a specific embodiment of the present invention, the invention comprises, by weight: 0.1-20 parts of cnidium monnieri extract, 0.005-10 parts of rosemary leaf oil and 70-99.895 parts of organic solvent.

[0013] In a specific embodiment of the present invention, the invention comprises, by weight: 0.5-15 parts of cnidium monnieri extract, 0.01-5 parts of rosemary leaf oil and 80-99.5 parts of organic solvent.

[0014] In a specific embodiment of the present invention, the invention comprises, by weight: 0.5-10 parts of cnidium monnieri extract, 0.01-1 parts of rosemary leaf oil and 89-99.5 parts of organic solvent.

[0015] In a specific embodiment of the present invention, the polyol comprises a first polyol with a content of ≤99.95% and a second polyol with a content of ≥0.05%, based on the weight percentage relative to the polyol.

[0016] In a specific embodiment of the present invention, the polyol comprises 80%-99.9% of the first polyol and 0.1%-20% of the second polyol, in terms of weight percentage relative to the polyol.

[0017] In a specific embodiment of the present invention, the polyol comprises 90%-99% of the first polyol and 0.1%-10% of the second polyol, in terms of weight percentage relative to the polyol.

[0018] The present invention also provides a method for preparing a composition with soothing effect, comprising: mixing cnidium monnieri extract, rosemary leaf oil and an organic solvent; wherein the organic solvent comprises a polyol or an oily solvent.

[0019] In a specific embodiment of the present invention, the polyol includes at least one of dipropylene glycol, propylene glycol, butylene glycol and glycerol.

[0020] In a specific embodiment of the present invention, the polyol includes a first polyol and a second polyol, the first polyol includes at least one of propylene glycol, butylene glycol, glycerol and dipropylene glycol, and the second polyol includes dipropylene glycol.

[0021] In one embodiment of the present invention, the polyol comprises dipropylene glycol and butylene glycol.

[0022] In one embodiment of the present invention, the polyol comprises dipropylene glycol.

[0023] In a specific embodiment of the present invention, the oily solvent comprises caprylic / capric triglyceride.

[0024] In a specific embodiment of the present invention, the composition comprises, by weight: 0.1-20 parts of cnidium monnieri extract, 0.005-10 parts of rosemary leaf oil and 70-99.895 parts of organic solvent.

[0025] In a specific embodiment of the present invention, the composition comprises, by weight: 0.5-15 parts of cnidium monnieri extract, 0.01-5 parts of rosemary leaf oil and 80-99.5 parts of organic solvent.

[0026] In a specific embodiment of the present invention, the composition comprises, by weight: 0.5-10 parts of cnidium monnieri extract, 0.01-1 parts of rosemary leaf oil and 89-99.5 parts of organic solvent.

[0027] In a specific embodiment of the present invention, the polyol comprises a first polyol with a content of ≤99.95% and a second polyol with a content of ≥0.05%, based on the weight percentage relative to the polyol.

[0028] In a specific embodiment of the present invention, the polyol comprises 80%-99.9% of the first polyol and 0.1%-20% of the second polyol, in terms of weight percentage relative to the polyol.

[0029] In a specific embodiment of the present invention, the polyol comprises 90%-99% of the first polyol and 0.1%-10% of the second polyol, in terms of weight percentage relative to the polyol.

[0030] In a specific embodiment of the present invention, the mixing process comprises: uniformly mixing the cnidium monnieri extract, rosemary leaf oil and an organic solvent, and filtering;

[0031] or,

[0032] The cnidium monnieri extract is mixed with an organic solvent and filtered to obtain a cnidium monnieri extract solution;

[0033] Mix the cnidium monnieri extract and rosemary leaf oil evenly.

[0034] In a specific embodiment of the present invention, the first mixing temperature ranges from 20-70°C, wherein the first mixing temperature is the temperature when the cnidium monnieri extract, rosemary leaf oil and organic solvent are mixed, or the temperature when the cnidium monnieri extract is mixed with the organic solvent.

[0035] In a specific embodiment of the present invention, the mixing process comprises:

[0036] The cnidium monnieri extract is mixed with the first polyol, and filtered to obtain a cnidium monnieri extract solution;

[0037] The rosemary leaf oil is mixed with a second polyol to obtain a rosemary mixed liquid;

[0038] Evenly mixing the cnidium monnieri extract and the rosemary mixture;

[0039] or,

[0040] The cnidium monnieri extract, rosemary leaf oil and a first polyol are mixed to obtain a first mixed solution;

[0041] The first mixed liquid and the second polyol are mixed uniformly.

[0042] In a specific embodiment of the present invention, the second mixing temperature ranges from 20-70°C, wherein the second mixing temperature is the temperature when the cnidium monnieri extract is mixed with the first polyol, or the temperature when the cnidium monnieri extract, rosemary leaf oil and the first polyol are mixed.

[0043] In a specific embodiment of the present invention, the preparation method of the Cnidium monnieri extract comprises:

[0044] Mix Cnidium monnieri and ethanol, extract at 60-90° C. for 1-4 hours, and filter; wherein the mass ratio of Cnidium monnieri to ethanol is 1:5-1:100.

[0045] In a specific embodiment of the present invention, the mass ratio of the Cnidium monnieri to ethanol is 1:10-1:50.

[0046] In a specific embodiment of the present invention, the mass fraction of ethanol is ≥50%.

[0047] In a specific embodiment of the present invention, the solution obtained after the filtration is concentrated.

[0048] The present invention also provides use of the above composition or the composition prepared by the above preparation method in preparing cosmetics.

[0049] Compared with the prior art, the present invention has the following beneficial effects:

[0050] (1) The composition of the present invention can solve the burning and stinging problems of sensitive skin at multiple targets, and can simultaneously and efficiently inhibit the expression of TRPV1 and TRPA1, shielding the key targets of burning and stinging, thereby achieving the purpose of quickly relieving burning and stinging; and the composition of the present invention has a synergistic effect on at least the inhibition of the TRPV1 target;

[0051] (2) The preparation method of the composition of the present invention is simple and easy to implement. The cnidium monnieri extract and rosemary essential oil are mixed with polyols or oily solvents to obtain a clear, homogeneous and stable composition. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0053] Figure 1 This is a comparison of the relative fluorescence intensity of the compositions of Examples 5, 8, 9, 10, and 11 of the present invention on the expression of TRPV1 in keratinocytes;

[0054] Figure 2 This is a graph showing the relative fluorescence intensity test result of the composition of Example 5 of the present invention on TRPA1 expression;

[0055] Figure 3 The figure is a test result of burning sensation after using the lotion containing the composition of Example 5 of the present invention. DETAILED DESCRIPTION

[0056] The technical scheme of the present invention will be clearly and completely described below in conjunction with the accompanying drawings and specific embodiments, but it will be understood by those skilled in the art that the following described embodiments are part of embodiments of the present invention, rather than all embodiments, and are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present invention. If specific conditions are not specified in the embodiments, they are carried out according to normal conditions or conditions recommended by the manufacturer. If the manufacturer is not specified in the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0057] In a specific embodiment of the present invention, a composition with soothing effect is provided, comprising: cnidium monnieri extract, rosemary leaf oil and an organic solvent; wherein the organic solvent comprises a polyol or an oily solvent.

[0058] Cnidium monnieri fruit extract is a substance obtained by extracting Cnidium monnieri fruit with a solvent under certain conditions. Cnidium monnieri fruit (Latin name: Cnidium monnieri (L.) Cuss.) is the dried mature fruit of the annual Umbelliferae plant Cnidium monnieri, which has a strong aromatic flavor. Among them, the Cnidium monnieri fruit extract can be a commercially available product, or it can be prepared. The Cnidium monnieri fruit extract can be in a dry powder state, an extract state, or a liquid state, such as an alcohol-soluble substance or a liquid phase system formed by dissolving in other solvents. The present invention is described by taking the Cnidium monnieri fruit extract in the extract state as an example. It is understandable that when the Cnidium monnieri fruit extract in the dry powder state or the liquid state is used, the amount taken can be adjusted accordingly.

[0059] Rosemary (Rosmainus officinalis L.) belongs to the genus Rosmarinus of the Lamiaceae family. Its main chemical components include 1,8-cineole, α-pinene, β-pinene, carnosic acid, carnosol, rosmarinic acid, rosmarinic phenol and other active compounds, and it has various physiological activities such as antioxidant, antibacterial, anti-inflammatory, analgesic, and anti-tumor. Rosemary leaf oil can be commercially available or prepared according to the current conventional method.

[0060] The composition of the present invention is clear and stable, and can solve the burning and stinging problems of sensitive skin at multiple targets. It can simultaneously inhibit the expression of TRPV1 and TRPA1, shield the key targets of burning and stinging, relieve inflammatory response, and achieve the purpose of quickly relieving burning and stinging, so as to solve the recurrence of skin sensitivity symptoms and relieve abnormal pain.

[0061] In a specific embodiment of the present invention, the polyol includes at least one of dipropylene glycol, propylene glycol, butylene glycol and glycerol. Among them, propylene glycol can be 1,2-butylene glycol and / or 1,3-propylene glycol, and butylene glycol can be at least one of 1,2-butylene glycol, 1,3-butylene glycol and 1,4-butylene glycol.

[0062] In a specific embodiment of the present invention, the polyol includes a first polyol and a second polyol; the first polyol includes at least one of propylene glycol, butylene glycol, glycerol and dipropylene glycol, and the second polyol includes dipropylene glycol.

[0063] In one embodiment of the present invention, the polyol includes dipropylene glycol and butylene glycol.

[0064] In one embodiment of the present invention, the polyol comprises dipropylene glycol.

[0065] The polyol may be only dipropylene glycol, or may include dipropylene glycol and other polyols. Among them, the polyol preferably includes dipropylene glycol and butylene glycol, which can effectively control the cost and obtain a clear and stable composition.

[0066] In one embodiment of the present invention, the oily solvent comprises caprylic / capric triglyceride (GTCC).

[0067] In a specific embodiment of the present invention, the composition comprises, by weight, 0.1-20 parts of cnidium monnieri extract, 0.005-10 parts of rosemary leaf oil, and 70-99.895 parts of an organic solvent. It is understood that the amount of cnidium monnieri extract and rosemary leaf oil in the composition can be adjusted according to actual needs, and can have a relatively higher amount, preferably maintained within the above range, so as to achieve the efficacy of the composition and avoid the loss of raw materials due to solubility problems.

[0068] In a specific embodiment of the present invention, the amount of Cnidium monnieri extract can be 0.1, 0.5, 1, 3, 5, 8, 10, 12, 15, 18 or 20 parts, or a number between any two of the above values.

[0069] In a specific embodiment of the present invention, the amount of rosemary leaf oil can be 0.005, 0.01, 0.05, 0.1, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 parts, or it can be a number between any two of the above values.

[0070] In a specific embodiment of the present invention, the amount of organic solvent used can be 70, 72, 75, 78, 80, 82, 85, 88, 90, 92, 95, 97, 99, 99.5 or 99.895 parts, or it can be a number between any two of the above values.

[0071] In a specific embodiment of the present invention, the composition comprises, by weight: 0.5-15 parts of cnidium monnieri extract, 0.01-5 parts of rosemary leaf oil and 80-99.5 parts of organic solvent.

[0072] In a specific embodiment of the present invention, the composition comprises, by weight: 0.5-10 parts of cnidium monnieri extract, 0.01-1 parts of rosemary leaf oil and 89-99.5 parts of organic solvent.

[0073] In a specific embodiment of the present invention, the polyol includes a first polyol with a content of ≤99.95% and a second polyol with a content of ≥0.05%, based on the weight percentage relative to the polyol. When the polyol includes the first polyol and the second polyol, the minimum amount of the second polyol is 0.05% of the total weight of the polyol, and the maximum amount of the second polyol is not limited.

[0074] In a specific embodiment of the present invention, the content of the first polyol can be 0, 0.05%, 0.1%, 0.5%, 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 99.95%, or a percentage content between any two of the above values. It is understood that when the content of the first polyol is 0, the polyol can only include the second polyol.

[0075] In a specific embodiment of the present invention, the content of the second polyol can be 0.05%, 0.1%, 0.5%, 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99.95% or 100%, or a percentage content between any two of the above values. It is understood that when the content of the second polyol is 100%, the polyol can only include the second polyol.

[0076] In a specific embodiment of the present invention, the polyol comprises 50%-99.95% of the first polyol and 0.05%-50% of the second polyol, based on the weight percentage relative to the polyol.

[0077] In one embodiment of the present invention, the polyol comprises 80%-99.9% of the first polyol and 0.1%-20% of the second polyol, in terms of weight percentage relative to the polyol;

[0078] In a specific embodiment of the present invention, the polyol comprises 90%-99.9% of the first polyol and 0.1%-10% of the second polyol, based on the weight percentage relative to the polyol.

[0079] In a specific embodiment of the present invention, a method for preparing a composition with soothing effect comprises: mixing a cnidium monnieri extract, rosemary leaf oil and an organic solvent; wherein the organic solvent comprises a polyol or an oily solvent. The polyol or oily solvent is used as the organic solvent, and the mixed system containing the cnidium monnieri extract and rosemary leaf oil is stable and clear.

[0080] It should be noted that, when preparing the composition, the amount of each substance in the composition, the specific types of polyols and oily solvents are the same as the substance types and amounts in the above composition, and will not be repeated here. During the mixing process, the organic solvent can be added once or multiple times.

[0081] In a specific embodiment of the present invention, the mixing process specifically comprises: uniformly mixing the cnidium monnieri extract, rosemary leaf oil and the organic solvent, and filtering.

[0082] When preparing the composition, the cnidium monnieri extract, rosemary leaf oil and organic solvent can be directly mixed and mixed to form a homogeneous and stable system. When the above substances are mixed, conventional mixing methods can be used, such as stirring, etc., and conventional mixing methods can be used unless otherwise specified. Filtration can be achieved in a conventional manner, such as filtering with SCP-333 type paperboard.

[0083] In a specific embodiment of the present invention, when the cnidium monnieri extract, rosemary leaf oil and organic solvent are directly mixed, a decolorization treatment is performed before filtering.

[0084] Specifically, after the cnidium monnieri extract, rosemary leaf oil and organic solvent are evenly mixed to obtain a mixed solution, the mixed solution can be mixed with a decolorizing agent. Among them, the decolorizing agent for decolorization may include at least one of activated carbon, hydrogen peroxide, resin, diatomaceous earth and alumina. The temperature and time of the decolorization treatment can be determined according to actual needs, usually at 20-80°C for about 0-5h. Preferably, the temperature range during the decolorization treatment is 40-70°C.

[0085] In a specific embodiment of the present invention, the temperature of the decolorization treatment can be 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75 or 80°C, or a temperature between any two of the above values.

[0086] In a specific embodiment of the present invention, the decolorization treatment time is 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5 hours, or it can be a time between any two of the above values.

[0087] In a specific embodiment of the present invention, the amount of the decolorizing agent used in the decolorization treatment is 0.1%-5% by weight of the composition, preferably 0.5%-3%.

[0088] In a specific embodiment of the present invention, the amount of the decolorizing agent can be 0.1%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5% or 5%, or it can be an amount between any two of the above values.

[0089] In a specific embodiment of the present invention, when the cnidium monnieri extract, rosemary leaf oil and organic solvent are mixed, the filtrate obtained can be sterilized after filtration. The sterilization method is a commonly used sterilization method in the cosmetics field, for example, it can be treated at 70-100°C for 0.1-3h.

[0090] In a specific embodiment of the present invention, the temperature range when the cnidium monnieri extract, rosemary essential oil and organic solvent are mixed is 20-70° C. The mixing time can be adjusted according to actual adaptability until the cnidium monnieri extract and rosemary leaf oil are uniformly and stably dispersed in the organic solvent; the mixing time is usually about 0.5-3h.

[0091] In a specific embodiment of the present invention, the mixing process comprises: mixing the cnidium monnieri extract with an organic solvent, filtering to obtain a cnidium monnieri extract;

[0092] Mix the cnidium monnieri extract and rosemary leaf oil evenly.

[0093] Can first cnidium monnieri extract be mixed with organic solvent, filter and obtain filtrate and be cnidium monnieri extract, then cnidium monnieri extract and rosemary leaf oil are mixed, can obtain composition.It is understandable that, after cnidium monnieri extract mixes with organic solvent, also can not filter first, filter again after cnidium monnieri extract and the mixed solution of organic solvent mix with rosemary leaf oil.Wherein, the time that cnidium monnieri extract mixes with organic solvent, and the time that cnidium monnieri extract mixes with rosemary leaf oil can be adjusted according to actual adaptability.

[0094] In a specific embodiment of the present invention, the temperature when the Cnidium monnieri extract and the organic solvent are mixed is preferably 20-70° C. The mixing time of the above reagents can be adjusted according to actual adaptability until the reagents are mixed evenly, and the mixing time is usually about 0.5-3 hours.

[0095] In a specific embodiment of the present invention, after the cnidium monnieri extract is mixed with the organic solvent, before filtering, a decolorization treatment may be performed. Wherein, the conditions for the decolorization treatment, including the type of reagent, temperature, time, etc. for the decolorization treatment, are the same as the conditions for the decolorization treatment when the cnidium monnieri extract, rosemary leaf oil and organic solvent are directly mixed, and will not be repeated here. It is understood that when the mixed solution obtained after the cnidium monnieri extract and the organic solvent are mixed, the amount of the decolorizing agent used during the decolorization treatment is 0.1%-5% of the total mass of the cnidium monnieri extract and the organic solvent, preferably 0.5%-3%.

[0096] In a specific embodiment of the present invention, before mixing the cnidium monnieri extract with the rosemary leaf oil, the cnidium monnieri extract may be sterilized, wherein the sterilization may be carried out by a conventional sterilization method, which will not be described in detail herein.

[0097] In a specific embodiment of the present invention, the mixing process comprises: mixing the cnidium monnieri extract with the first polyol, and filtering to obtain a cnidium monnieri extract;

[0098] mixing rosemary leaf oil with a second polyol to obtain a rosemary mixed liquid;

[0099] Mix the cnidium monnieri extract and rosemary mixture evenly;

[0100] When the polyol includes the first polyol and the second polyol, the cnidium monnieri extract can be mixed with the first polyol first, and the obtained filtrate is filtered to obtain the cnidium monnieri extract. Rosemary leaf oil is mixed with the second polyol to obtain a rosemary mixed solution. The cnidium monnieri extract and the rosemary mixed solution are then mixed uniformly. It is understandable that after the cnidium monnieri extract is mixed with the first polyol, it is also possible not to filter, but directly to mix with the rosemary mixed solution, and then to filter and process.

[0101] The temperature when the cnidium monnieri extract and the first polyol are mixed is 20-70°C. The mixing time of the above two reagents is not limited and can be determined according to actual needs, usually about 0.5-3h. Rosemary leaf oil and the second polyol can be mixed at room temperature, and the cnidium monnieri extract and the rosemary mixture can also be mixed at room temperature. The mixing time can be unlimited, and a homogeneous system can be formed.

[0102] It is understandable that in this specific embodiment, the cnidium monnieri extract and the second polyol may be mixed to obtain a cnidium monnieri extract; the rosemary leaf oil may be uniformly mixed with the first polyol to obtain a rosemary mixed solution; and the cnidium monnieri extract and the rosemary mixed solution may be uniformly mixed. That is, when the polyol includes the first polyol and the second polyol, the type of polyol mixed with the cnidium monnieri extract or the rosemary leaf oil does not need to be specifically limited.

[0103] When GTCC is used as the organic solvent, the mixing treatment can also be that the cnidium monnieri extract is mixed with GTCC to obtain a cnidium monnieri extract solution, the rosemary leaf oil is mixed with GTCC to obtain a rosemary mixed solution, and then the cnidium monnieri extract solution and the rosemary mixed solution are mixed evenly.

[0104] In a specific embodiment of the present invention, after the cnidium monnieri extract is mixed with the first polyol, a decolorization treatment may be performed before filtering. The conditions for the decolorization treatment, including the type of reagent, dosage, temperature, time, etc. for the decolorization treatment, are the same as those for the decolorization treatment when the cnidium monnieri extract, rosemary leaf oil and organic solvent are directly mixed, and will not be repeated here. It is understood that when the mixed solution obtained by mixing the cnidium monnieri extract and the first polyol is decolorized, the amount of the decolorizing agent used during the decolorization treatment is 0.1%-5% of the total mass of the cnidium monnieri extract and the first polyol, preferably 0.5%-3%.

[0105] In a specific embodiment of the present invention, before mixing the cnidium monnieri extract with the rosemary mixture, the cnidium monnieri extract may be sterilized, wherein the sterilization may be carried out by a conventional sterilization method, which will not be described in detail herein.

[0106] In a specific embodiment of the present invention, the mixing process includes: mixing the cnidium monnieri extract, rosemary leaf oil and a first polyol to obtain a first mixed solution;

[0107] The first mixed liquid and the second polyol are mixed uniformly.

[0108] The cnidium monnieri extract and rosemary leaf oil can be first mixed with the first polyol to obtain a first mixed solution; and then the first mixed solution is evenly mixed with the second polyol. The temperature when the cnidium monnieri extract, rosemary leaf oil and the first polyol are mixed is preferably 20-70°C, and the mixing time can be determined according to actual needs, usually about 0.5-3h. The first mixed solution and the second polyol can be mixed at room temperature, and the time is not specifically limited. Whether to filter the mixture is determined according to actual conditions to form a homogeneous and stable system.

[0109] It should be noted that, in this specific embodiment, the cnidium monnieri extract and rosemary leaf oil may also be first mixed with the second polyol to obtain a first mixed solution; the first mixed solution is then uniformly mixed with the first polyol. That is, when the polyol includes the first polyol and the second polyol, the polyol first mixed with the cnidium monnieri extract and rosemary leaf oil may be the first polyol, the second polyol, or the first polyol and the second polyol.

[0110] In a specific embodiment of the present invention, after the cnidium monnieri extract, rosemary leaf oil and the first polyol are mixed, a decolorization treatment may be performed. The conditions for the decolorization treatment are not repeated. It is understood that the amount of the decolorizing agent during the decolorization treatment may be 0.1%-5% of the total mass of the cnidium monnieri extract, rosemary leaf oil and the first polyol, preferably 0.5%-3%.

[0111] In a specific embodiment of the present invention, the first mixed solution may be sterilized, or the first mixed solution and the second polyol may be sterilized after being uniformly mixed. The specific sterilization method and conditions are not described in detail.

[0112] In a specific embodiment of the present invention, the preparation method of the Cnidium monnieri extract comprises: mixing Cnidium monnieri with ethanol, extracting at 60-90°C for 1-4h, and filtering; wherein the mass ratio of Cnidium monnieri to ethanol is 1:5-1:100. Wherein, the extraction temperature can be 60, 65, 70, 75, 80, 85 or 90°C, etc., or a temperature between any two of the above values. It should be noted that the Cnidium monnieri extract of the present invention can be a commercially available product, can be prepared by the above method, or can be prepared by other preparation methods; the Cnidium monnieri extract of the present invention is preferably an alcohol extract of Cnidium monnieri.

[0113] In a specific embodiment of the present invention, the mass ratio of Cnidium monnieri to ethanol is 1:5, 1:10, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50, 1:60, 1:70, 1:80, 1:90 or 1:100, or the ratio between any two of the above ratios. It is understood that when Cnidium monnieri is extracted, the amount of ethanol may be lower or higher, preferably maintained within the above range from the perspective of extraction efficiency / effect.

[0114] In a specific embodiment of the present invention, the mass ratio of Cnidium monnieri to ethanol is 1:10-1:50.

[0115] In a specific embodiment of the present invention, the mass ratio of Cnidium monnieri to ethanol is 1:10-1:30.

[0116] In a specific embodiment of the present invention, the mass fraction of ethanol is ≥50%, wherein the mass fraction of ethanol may be further preferably ≥70%.

[0117] In a specific embodiment of the present invention, the preparation method of Cnidium monnieri extract further comprises: concentrating the solution obtained after filtration. The concentration method can be a rotary evaporation concentration or a commonly used concentration method in the art. Specifically, when the rotary evaporation concentration is used, the treatment temperature is in the range of 40-70°C.

[0118] In a specific embodiment of the present invention, the above composition or the composition prepared by the above preparation method is used in the preparation of cosmetics. The cosmetics can be common types in the cosmetics field, such as lotions, emulsions, creams, masks, sunscreens, makeup products, etc., and can also be shampoo products, bath products, etc.

[0119] The following embodiments and experimental examples are used to illustrate the technical solutions of the present invention.

[0120] Example 1

[0121] The preparation method of cnidium monnieri extract comprises:

[0122] 1) Mixing Cnidium monnieri and 95% ethanol aqueous solution, extracting at 80° C. for 2 h to obtain a first crude extract; filtering the first crude extract at 30° C. to remove residues, to obtain a second crude extract; wherein the mass ratio of Cnidium monnieri to ethanol aqueous solution is 1:20;

[0123] 2) The second crude extract was concentrated at 50° C. using a rotary vacuum concentration device to obtain a cnidium monnieri extract.

[0124] Example 2

[0125] The preparation method of cnidium monnieri extract comprises:

[0126] 1) Mixing Cnidium monnieri and 50% ethanol aqueous solution, extracting at 90° C. for 3 h to obtain a first crude extract; filtering the first crude extract at 30° C. to remove residues, to obtain a second crude extract; wherein the mass ratio of Cnidium monnieri to ethanol aqueous solution is 1:40;

[0127] 2) The second crude extract was concentrated at 70° C. using a rotary vacuum concentration device to obtain a cnidium monnieri extract.

[0128] Example 3

[0129] The preparation method of cnidium monnieri extract comprises:

[0130] 1) Mixing Cnidium monnieri and 70% ethanol aqueous solution, extracting at 60° C. for 1 h to obtain a first crude extract; filtering the first crude extract at 30° C. to remove residues, to obtain a second crude extract; wherein the mass ratio of Cnidium monnieri to ethanol aqueous solution is 1:10;

[0131] 2) The second crude extract was concentrated at 40° C. using a rotary vacuum concentration device to obtain a cnidium monnieri extract.

[0132] Example 4

[0133] The preparation method of cnidium monnieri extract comprises:

[0134] 1) Mixing Cnidium monnieri and 95% ethanol aqueous solution, extracting at 80° C. for 2 h to obtain a first crude extract; filtering the first crude extract at 30° C. to remove residues, to obtain a second crude extract; wherein the mass ratio of Cnidium monnieri to ethanol aqueous solution is 1:50;

[0135] 2) The second crude extract was concentrated at 60° C. using a rotary vacuum concentration device to obtain a cnidium monnieri extract.

[0136] Example 5

[0137] A method for preparing a composition having a soothing effect, comprising:

[0138] 1) The cnidium monnieri extract prepared by the preparation method of Example 1 is redissolved with 100% by mass of 1,3-butanediol to obtain a redissolved mixed solution; wherein the mass ratio of the cnidium monnieri extract to 1,3-butanediol is 1:20;

[0139] 2) adding activated carbon to the reconstituted mixed solution, decolorizing at 50° C. for 2 h, filtering, and sterilizing at 80° C. for 1 h to obtain a reconstituted solution; wherein the amount of activated carbon is 1.5% by mass of the reconstituted mixed solution;

[0140] 3) 2 g of rosemary leaf oil (supplier: Guangzhou Yicheng Essential Oil Co., Ltd.) and 3 g of 100% by mass dipropylene glycol were mixed and stirred evenly at room temperature to obtain a rosemary mixed solution;

[0141] 4) The rosemary mixture in step 3) was mixed with 995 g of the reconstituted solution in step 2), and stirred evenly at room temperature.

[0142] Example 6

[0143] A method for preparing a composition having a soothing effect, comprising:

[0144] 1) The cnidium monnieri extract prepared by the preparation method of Example 1 is redissolved with 100% by mass of 1,3-butanediol to obtain a redissolved mixed solution; wherein the mass ratio of the cnidium monnieri extract to 1,3-butanediol is 1:20;

[0145] 2) adding activated carbon to the reconstituted mixed solution, performing a decolorization treatment at 70° C. for 30 min, filtering, and sterilizing at 80° C. for 1 h to obtain a reconstituted solution; wherein the amount of activated carbon is 2% of the mass of the reconstituted mixed solution;

[0146] 3) Mix 995 g of the reconstituted solution in step 2) and 3 g of 100% by mass dipropylene glycol, and stir evenly at room temperature to obtain a Cnidium monnieri mixed solution;

[0147] 4) Mix the Cnidium monnieri mixture in step 3) with 2 g of rosemary leaf oil, and stir evenly at room temperature.

[0148] Example 7

[0149] A method for preparing a composition having a soothing effect, comprising:

[0150] 1) The cnidium monnieri extract prepared by the preparation method of Example 1 is redissolved with 100% by mass of 1,3-butanediol to obtain a redissolved mixed solution; wherein the mass ratio of the cnidium monnieri extract to 1,3-butanediol is 1:20;

[0151] 2) adding activated carbon to the reconstituted mixed solution, performing a decolorization treatment at 20° C. for 3 h, filtering, and sterilizing at 80° C. for 1 h to obtain a reconstituted solution; wherein the amount of activated carbon used is 0.2% of the mass of the reconstituted mixed solution;

[0152] 3) mixing 995 g of the reconstituted solution in step 2) and 2 g of rosemary leaf oil, and stirring at room temperature to obtain a mixed solution;

[0153] 4) The mixed solution in step 3) was mixed with 3 g of 100% by mass dipropylene glycol, and stirred evenly at room temperature.

[0154] Example 8

[0155] A method for preparing a composition having a soothing effect, comprising:

[0156] 1) The cnidium monnieri extract prepared by the preparation method of Example 1 is redissolved with 100% by mass of 1,3-butanediol to obtain a redissolved mixed solution; wherein the mass ratio of the cnidium monnieri extract to 1,3-butanediol is 1:20;

[0157] 2) adding activated carbon to the reconstituted mixed solution, performing a decolorization treatment at 60° C. for 1.5 h, filtering, and sterilizing at 80° C. for 1 h to obtain a reconstituted solution; wherein the amount of activated carbon used is 1% by mass of the reconstituted mixed solution;

[0158] 3) 0.1 g of rosemary leaf oil and 1 g of 100% by mass dipropylene glycol were mixed and stirred evenly at room temperature to obtain a rosemary mixed solution;

[0159] 4) The rosemary mixture in step 3) was mixed with 998.9 g of the reconstituted solution in step 2), and stirred evenly at room temperature.

[0160] Example 9

[0161] A method for preparing a composition having a soothing effect, comprising:

[0162] 1) The cnidium monnieri extract prepared by the preparation method of Example 1 is redissolved with 100% by mass of 1,3-butanediol to obtain a redissolved mixed solution; wherein the mass ratio of the cnidium monnieri extract to 1,3-butanediol is 1:20;

[0163] 2) adding activated carbon to the reconstituted mixed solution, decolorizing at 40° C. for 1.5 h, filtering, and sterilizing at 80° C. for 1 h to obtain a reconstituted solution; wherein the amount of activated carbon is 2% by mass of the reconstituted mixed solution;

[0164] 3) 10 g of rosemary leaf oil and 100 g of 100% by mass dipropylene glycol were mixed and stirred evenly at room temperature to obtain a rosemary mixed solution;

[0165] 4) The rosemary mixture in step 3) was mixed with 890 g of the reconstituted solution in step 2), and stirred evenly at room temperature.

[0166] Example 10

[0167] A method for preparing a composition having a soothing effect, comprising:

[0168] 1) The cnidium monnieri extract prepared by the preparation method of Example 1 is redissolved with 100% by mass of 1,3-butanediol to obtain a redissolved mixed solution; wherein the mass ratio of the cnidium monnieri extract to 1,3-butanediol is 1:20;

[0169] 2) adding activated carbon to the reconstituted mixed solution, decolorizing at 60° C. for 1.5 h, filtering, and sterilizing at 80° C. for 1 h to obtain a reconstituted solution; wherein the amount of activated carbon is 1% by mass of the reconstituted mixed solution;

[0170] 3) 5 g of rosemary leaf oil and 10 g of 100% by mass dipropylene glycol were mixed and stirred evenly at room temperature to obtain a rosemary mixed solution;

[0171] 4) The rosemary mixture in step 3) was mixed with 985 g of the reconstituted solution in step 2), and stirred evenly at room temperature.

[0172] Embodiment 11

[0173] A method for preparing a composition having a soothing effect, comprising:

[0174] 1) The cnidium monnieri extract prepared by the preparation method of Example 1 is redissolved with 100% by mass of 1,3-butanediol to obtain a redissolved mixed solution; wherein the mass ratio of the cnidium monnieri extract to 1,3-butanediol is 1:100;

[0175] 2) adding activated carbon to the reconstituted mixed solution, performing a decolorization treatment at 60° C. for 1.5 h, filtering, and sterilizing at 80° C. for 1 h to obtain a reconstituted solution; wherein the amount of activated carbon used is 1% by mass of the reconstituted mixed solution;

[0176] 3) 2 g of rosemary leaf oil and 3 g of 100% by mass dipropylene glycol were mixed and stirred evenly at room temperature to obtain a rosemary mixed solution;

[0177] 4) The rosemary mixture in step 3) was mixed with 995 g of the reconstituted solution in step 2), and stirred evenly at room temperature.

[0178] Example 12

[0179] A method for preparing a composition having a soothing effect, comprising:

[0180] 1) The cnidium monnieri extract prepared by the preparation method of Example 1 is redissolved with 100% by mass of 1,3-butanediol to obtain a redissolved mixed solution; wherein the mass ratio of the cnidium monnieri extract to 1,3-butanediol is 1:20;

[0181] 2) adding activated carbon to the reconstituted mixed solution, performing a decolorization treatment at 60° C. for 1.5 h, filtering, and sterilizing at 80° C. for 1 h to obtain a reconstituted solution; wherein the amount of activated carbon used is 1% by mass of the reconstituted mixed solution;

[0182] 3) 3 g of rosemary leaf oil and 7 g of 100% by mass dipropylene glycol were mixed and stirred evenly at room temperature to obtain a rosemary mixed solution;

[0183] 4) The rosemary mixture in step 3) was mixed with 990 g of the reconstituted solution in step 2), and stirred evenly at room temperature.

[0184] Embodiment 13

[0185] A method for preparing a composition having a soothing effect, comprising:

[0186] 1) The cnidium monnieri extract prepared by the preparation method of Example 1 is re-dissolved in 100% by mass glycerol to obtain a re-dissolved mixed solution; wherein the mass ratio of the cnidium monnieri extract to the glycerol is 1:5;

[0187] 2) adding activated carbon to the reconstituted mixed solution, performing a decolorization treatment at 60° C. for 1.5 h, filtering, and sterilizing at 80° C. for 1 h to obtain a reconstituted solution; wherein the amount of activated carbon used is 1% by mass of the reconstituted mixed solution;

[0188] 3) 2 g of rosemary leaf oil and 3 g of 100% by mass dipropylene glycol were mixed and stirred evenly at room temperature to obtain a rosemary mixed solution;

[0189] 4) The rosemary mixture in step 3) was mixed with 995 g of the reconstituted solution in step 2), and stirred evenly at room temperature.

[0190] Embodiment 14

[0191] A method for preparing a composition having a soothing effect, comprising:

[0192] 1) The cnidium monnieri extract prepared by the preparation method of Example 1 is redissolved with 100% by mass of 1,3-propylene glycol to obtain a redissolved mixed solution; wherein the mass ratio of the cnidium monnieri extract to 1,3-propylene glycol is 1:10;

[0193] 2) adding activated carbon to the reconstituted mixed solution, performing a decolorization treatment at 60° C. for 1.5 h, filtering, and sterilizing at 80° C. for 1 h to obtain a reconstituted solution; wherein the amount of activated carbon used is 1% by mass of the reconstituted mixed solution;

[0194] 3) 2 g of rosemary leaf oil and 3 g of 100% by mass dipropylene glycol were mixed and stirred evenly at room temperature to obtain a rosemary mixed solution;

[0195] 4) The rosemary mixture in step 3) was mixed with 995 g of the reconstituted solution in step 2), and stirred evenly at room temperature.

[0196] Embodiment 15

[0197] A method for preparing a composition having a soothing effect, comprising:

[0198] 1) The cnidium monnieri extract prepared by the preparation method of Example 1 is redissolved with 100% by mass of 1,3-butanediol to obtain a redissolved mixed solution; wherein the mass ratio of the cnidium monnieri extract to 1,3-butanediol is 1:50;

[0199] 2) adding activated carbon to the reconstituted mixed solution, performing a decolorization treatment at 60° C. for 1.5 h, filtering, and sterilizing at 80° C. for 1 h to obtain a reconstituted solution; wherein the amount of activated carbon used is 1% by mass of the reconstituted mixed solution;

[0200] 3) 2 g of rosemary leaf oil and 3 g of 100% by mass dipropylene glycol were mixed and stirred evenly at room temperature to obtain a rosemary mixed solution;

[0201] 4) The rosemary mixture in step 3) was mixed with 995 g of the reconstituted solution in step 2), and stirred evenly at room temperature.

[0202] Example 16

[0203] A method for preparing a composition having a soothing effect, comprising:

[0204] 180 g of the Cnidium monnieri extract prepared by the preparation method of Example 1, 50 g of rosemary leaf oil and 770 g of 100% by mass dipropylene glycol were mixed, stirred evenly at room temperature, and filtered.

[0205] Embodiment 17

[0206] A method for preparing a composition having a soothing effect, comprising:

[0207] 100 g of commercially available Cnidium monnieri extract (provided by Beijing Miaosen Biotechnology Co., Ltd.), 100 g of rosemary leaf oil and 800 g of 100% by mass dipropylene glycol were mixed, stirred evenly at room temperature, and filtered.

[0208] Embodiment 18

[0209] A method for preparing a composition having a soothing effect, comprising:

[0210] 0.05 g of commercially available Cnidium monnieri extract (Beijing Dongfang Miaosen Biotechnology Co., Ltd.), 2 g of rosemary leaf oil and 997.95 g of 100% GTCC were mixed, stirred evenly at room temperature and filtered.

[0211] Comparative Example 1

[0212] The composition was prepared in the same manner as in Example 9, except that in step 3), 10 g of rosemary leaf oil and 100 g of PEG-40 hydrogenated castor oil were mixed.

[0213] Comparative Example 2

[0214] The composition was prepared in the same manner as in Example 9, except that in step 3), 10 g of rosemary leaf oil and 100 g of Tween-20 were mixed.

[0215] Comparative Example 3

[0216] The composition was prepared in the same manner as in Example 9, except that:

[0217] In step 3), 10 g of rosemary leaf oil, 100 g of PEG-40 hydrogenated castor oil and 100 g of Tween-20 were mixed;

[0218] In step 4), the rosemary mixture in step 3) is mixed with 790 g of the reconstituted solution in step 2).

[0219] Experimental Example 1 Turbidity Detection

[0220] The turbidity index of some sample solutions was tested by using a turbidity analyzer. Please see Table 1 for details.

[0221] Table 1 Turbidity data of samples obtained from Examples 5-11 and Comparative Examples 1-3

[0222] sample Turbidity (stock solution, FNU) Example 5 1.12 Example 6 1.68 Example 7 0.98 Example 8 1.57 Example 9 2.46 Example 10 1.86 Embodiment 11 1.05 Comparative Example 1 432 Comparative Example 2 28.7 Comparative Example 3 20.1

[0223] As can be seen from the data in Table 1, the composition containing cnidium monnieri extract and rosemary leaf oil of the present invention has good stability and does not have precipitation phenomenon. By appearance observation, the composition of the present invention is transparent, clear and homogeneous and stable. In addition to the samples obtained by the above-mentioned embodiments, the samples obtained by Examples 12-18 of the present invention are also stable, transparent and clear homogeneous products. At the same time, as can be seen from the data in Table 1, when cnidium monnieri extract, rosemary leaf oil are mixed with PEG-40 hydrogenated castor oil and / or Tween-20 reagent, the turbidity data of the obtained samples (products of Comparative Examples 1-3) are significantly increased; The observation results show obvious precipitation phenomenon.

[0224] It should also be noted that when the composition contains cnidium monnieri extract, rosemary leaf oil, polyol (dipropylene glycol, butylene glycol, propylene glycol or glycerol) and GTCC at the same time, the resulting composition shows obvious stratification phenomenon, and a homogeneous and stable composition cannot be obtained.

[0225] Experimental Example 2 TRPV1 expression test

[0226] Test method:

[0227] ① Inoculation: Keratinocytes were inoculated into 24-well plates and incubated in a 37°C, 5% CO2 incubator for 24 h.

[0228] ② Capsaicin stimulation: After 24 hours of culture, the culture medium in the plate was aspirated, and PBS was added to wash once. The blank control group (BC) was added with cell culture medium, and the negative control group (NC), sample group and positive control group (PC) were added with cell culture medium containing capsaicin, and cultured in a 37°C, 5% CO2 incubator for 24 hours, and then the supernatant was collected. The concentration of each sample added is shown in Table 2. The sample groups correspond to the tests of the five samples of Examples 5, 8, 9, 10 and 11, and the sample concentrations are all 0.1%.

[0229] ③ Sampling: According to the experimental grouping and concentration settings in Table 2, after the cells were plated and grown in the 24-well plate for 24 hours, group sampling was carried out, with 3 replicate wells in each group, and culture was continued in a 37°C, 5% CO2 incubator for 24 hours.

[0230] Table 2 TRPV1 test administration concentrations of samples of Examples 5, 8, 9, 10, 11 and control group samples

[0231]

[0232] ④TRPV1 detection: Immunofluorescence staining was performed, washed with PBS three times, fixed with 4% paraformaldehyde for 30 minutes, washed with PBS three times, permeabilized with 0.5% Triton X-100 for 20 minutes, washed with PBS three times, blocked with 5% bovine serum albumin (BSA) at room temperature for 60 minutes, and the primary antibody was incubated at 4°C overnight. The next day, washed with PBS three times, incubated with secondary antibody for 1 hour, washed with PBS three times, and observed and photographed under a fluorescence microscope.

[0233] ⑤Data processing: ImageJ software was used to process the fluorescence photos and calculate the relative IOD (cumulative optical density) average. The data obtained were processed and plotted using Excel software. SPSS17. was used for statistical analysis. One-way analysis of variance (ANOVA) was used for inter-group comparison. When P < 0.05, the difference was considered significant. For specific data, please refer to Table 3 and Figure 1 .

[0234] Table 3 TRPV1 test administration concentrations of samples of Examples 5, 8, 9, 10, 11 and control group samples

[0235]

[0236] See also Figure 1 As shown in the figure, "**" indicates p < 0.01, which is a significant difference. Figure 1 It can be seen from the data that compared with the negative control (NC), the compositions obtained in Examples 5, 8, 9, 10, and 11 can significantly inhibit the expression of TRPV1 caused by capsaicin, indicating that the compositions obtained in Examples 5, 8, 9, 10, and 11 have good soothing effects. In addition, the main active ingredient of rosemary leaf oil is 1,8-cineole, and existing studies have shown that 1,8-cineole does not have the effect of inhibiting the expression of TRPV1; however, from Tables 3 and Figure 1 It can also be seen that when the relative content of rosemary leaf oil in the composition is high, the inhibitory effect of the obtained composition on the expression of TRPV1 is significantly improved; this shows that the composition containing cnidium monnieri extract and rosemary leaf oil of the present invention has a synergistic effect in inhibiting the expression of TRPV1.

[0237] Experimental Example 3 TRPA1 expression test

[0238] Experimental steps:

[0239] 1) Cell inoculation: After the cells are revived, when the plating rate reaches about 60%, the cells are inoculated into a 24-well plate and incubated overnight in a CO2 incubator (37°C, 5% CO2).

[0240] 2) Solution preparation: Prepare the working solution of the test substance according to the test groups in Table 4.

[0241] Table 4 TRPA1 test administration concentrations of samples in Example 5 and control group samples

[0242]

[0243] 3) Drug administration and stimulation: According to the test grouping, when the cell plating rate in the 24-well plate reaches 40% to 60%, group drug administration and stimulation are carried out, and 3 replicate wells are set up for each group. 1 mL of culture medium is added to each well of the blank control group, 1 mL of culture medium containing salicylic acid is added to each well of the negative control group, 1 mL of culture medium containing salicylic acid and trans-4-tert-butyl cyclohexanol is added to each well of the positive control group, and 1 mL of culture medium containing salicylic acid and the corresponding concentration of the sample to be tested is added to each well of the sample group. After drug administration, the 24-well plate is placed in a CO2 incubator (37°C, 5% CO2) and cultured for 24 hours.

[0244] 4) Immunofluorescence test: Fix the cells with 4% paraformaldehyde for 30 minutes, then perform immunofluorescence detection. Observe the staining results under a fluorescence microscope and take pictures. Collect the pictures and analyze them.

[0245] 5) Statistical analysis of results: GraphPad Prism was used for plotting, and the results were expressed as Mean ± SD. The t-test was used for statistical analysis between the groups. All statistical analyses were two-tailed. P < 0.05 was considered to be significantly different, and P < 0.01 was considered to be extremely significantly different.

[0246] See also Figure 2 As shown, compared with the NC group, the composition of Example 5 has an inhibitory effect on the expression of TRPA1 protein and has a good soothing effect.

[0247] Experimental Example 4 Human Capsaicin Test

[0248] Experimental steps:

[0249] The soothing effect was tested through a capsaicin stimulation test. During the test, filter paper pieces (about 0.8 cm in diameter) soaked in 50 μL of 0.01% capsaicin aqueous solution were applied to the nasolabial grooves on both sides of the subject at the same time. When the subject felt that the sensation scores at the nasolabial grooves on both sides were ≥ 3 points (moderate sensation and above), the filter paper was removed.

[0250] The test personnel applied test sample 1 on one side of the test area and test sample 2 on the other side, and the amount of the sample was 50 μL. Among them, the test sample 1 (referred to as 2% Example 5 emulsion) is an emulsion containing 2% of the composition obtained in Example 5 by mass (the emulsion matrix also contains 0.1% xanthan gum, 0.05% disodium ethylenediaminetetraacetic acid, 5% glycerol, 2% butylene glycol, 3.5% sucrose stearate (and) cetearyl glucoside (and) cetyl alcohol (EC-FIX SE), 1% glyceryl stearate (A170), 8% caprylic / capric triglyceride (GTCC), 2% polydimethylsiloxane (DM200), 0.6% phenoxyethanol (and) ethylhexylglycerol (PEHG), and the rest is water), and the test sample 2 (referred to as blank matrix) has the same composition as the test sample 1, but does not contain the composition of Example 5.

[0251] Before using the test samples (after capsaicin stimulation, before using the test samples), and 0.5min, 5min, 10min, 15min, 20min and 30min after using the test samples, the subjects subjectively evaluated the burning pain in the nasolabial grooves on both sides and scored them on a 5-point scale. The scoring criteria are: 0 point for no feeling, 1 point for barely noticeable, 2 points for mildly noticeable, 3 points for moderately noticeable, 4 points for severely noticeable, and 5 points for pain.

[0252] See also Figure 3 As shown, at 0.5 min, 5 min, 10 min and 20 min after using the test sample, the average burning pain score of the test area applied with 2% of the emulsion of Example 5 was lower than that of the test area applied with the blank matrix.

[0253] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition with soothing effect, characterized in that: include: Cnidium monnieri extract, rosemary leaf oil and an organic solvent; wherein the organic solvent comprises polyol or an oily solvent.

2. The composition according to claim 1, characterized in that The polyol includes at least one of dipropylene glycol, propylene glycol, butylene glycol and glycerol; Preferably, the polyol comprises a first polyol and a second polyol; the first polyol comprises at least one of propylene glycol, butylene glycol, glycerol and dipropylene glycol, and the second polyol comprises dipropylene glycol; Preferably, the polyol comprises dipropylene glycol and butylene glycol; Preferably, the polyol comprises dipropylene glycol; Preferably, the oily solvent comprises caprylic / capric triglyceride.

3. The composition according to claim 1, characterized in that In parts by weight, the composition comprises: 0.1-20 parts of cnidium monnieri extract, 0.005-10 parts of rosemary leaf oil and 70-99.895 parts of an organic solvent; Preferably, the composition comprises, by weight: 0.5-15 parts of cnidium monnieri extract, 0.01-5 parts of rosemary leaf oil and 80-99.5 parts of an organic solvent; Preferably, the composition comprises, by weight: 0.5-10 parts of cnidium monnieri extract, 0.01-1 parts of rosemary leaf oil and 89-99.5 parts of an organic solvent.

4. The composition according to claim 2, characterized in that The polyol comprises, in terms of weight percentage relative to the polyol, a first polyol having a content of ≤99.95% and a second polyol having a content of ≥0.05%; Preferably, the polyol comprises 80%-99.9% of the first polyol and 0.1%-20% of the second polyol, in terms of weight percentage relative to the polyol; Preferably, the polyol comprises 90%-99.9% of the first polyol and 0.1%-10% of the second polyol, in terms of weight percentage relative to the polyol.

5. A method for preparing a composition having a soothing effect, characterized in that: include: The cnidium monnieri extract, rosemary leaf oil and an organic solvent are mixed and treated; wherein the organic solvent comprises polyol or an oily solvent.

6. The preparation method according to claim 5, characterized in that: The polyol includes at least one of dipropylene glycol, propylene glycol, butylene glycol and glycerol; Preferably, the polyol comprises a first polyol and a second polyol, the first polyol comprises at least one of propylene glycol, butylene glycol, glycerol and dipropylene glycol, and the second polyol comprises dipropylene glycol; Preferably, the polyol comprises dipropylene glycol and butylene glycol; Preferably, the polyol comprises dipropylene glycol; Preferably, the oily solvent comprises caprylic / capric triglyceride; Preferably, the composition comprises, by weight: 0.1-20 parts of cnidium monnieri extract, 0.005-10 parts of rosemary leaf oil and 70-99.895 parts of an organic solvent; Preferably, the composition comprises, by weight: 0.5-15 parts of cnidium monnieri extract, 0.01-5 parts of rosemary leaf oil and 80-99.5 parts of an organic solvent; Preferably, the composition comprises, by weight: 0.5-10 parts of cnidium monnieri extract, 0.01-1 parts of rosemary leaf oil and 89-99.5 parts of an organic solvent; Preferably, the polyol comprises a first polyol having a content of ≤99.95% and a second polyol having a content of ≥0.05%, in terms of weight percentage relative to the polyol; Preferably, the polyol comprises 80%-99.9% of the first polyol and 0.1%-20% of the second polyol, in terms of weight percentage relative to the polyol; Preferably, the polyol comprises 90%-99.9% of the first polyol and 0.1%-10% of the second polyol, in terms of weight percentage relative to the polyol.

7. The preparation method according to claim 5, characterized in that: The mixing process comprises: uniformly mixing the cnidium monnieri extract, rosemary leaf oil and an organic solvent, and filtering; or, The cnidium monnieri extract is mixed with an organic solvent and filtered to obtain a cnidium monnieri extract solution; Mix cnidium monnieri extract and rosemary leaf oil evenly; Preferably, the first mixing temperature ranges from 20-70° C., wherein the first mixing temperature is the temperature when the cnidium monnieri extract, rosemary leaf oil and organic solvent are mixed, or the temperature when the cnidium monnieri extract and organic solvent are mixed.

8. The preparation method according to claim 6, characterized in that: The mixing process includes: The cnidium monnieri extract is mixed with the first polyol, and filtered to obtain a cnidium monnieri extract solution; The rosemary leaf oil is mixed with a second polyol to obtain a rosemary mixed liquid; Evenly mixing the cnidium monnieri extract and the rosemary mixture; or, The cnidium monnieri extract, rosemary leaf oil and a first polyol are mixed to obtain a first mixed solution; uniformly mixing the first mixed liquid and the second polyol; Preferably, the second mixing temperature is in the range of 20-70° C., wherein the second mixing temperature is the temperature when the cnidium monnieri extract is mixed with the first polyol, or the temperature when the cnidium monnieri extract, rosemary leaf oil and the first polyol are mixed.

9. The preparation method according to claim 5, characterized in that: The preparation method of the Cnidium monnieri extract comprises: Mix Cnidium monnieri and ethanol, extract at 60-90°C for 1-4h, and filter; wherein the mass ratio of Cnidium monnieri to ethanol is 1:5-1:100; Preferably, the mass ratio of the Cnidium monnieri to ethanol is 1:10-1:50; Preferably, the mass fraction of ethanol is ≥50%; Preferably, the solution obtained after the filtration is concentrated.

10. Use of the composition according to any one of claims 1 to 4 or the composition prepared according to the preparation method according to any one of claims 5 to 9 in the preparation of cosmetics.

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