Device for comparing variation characteristics of root exudates and rhizosphere microorganisms of juglans sigillata along altitude gradient

Abstract

The invention discloses a device for comparing variation characteristics of root exudates and rhizosphere microorganisms of pholiota nameko along altitude gradient, the device comprises a gradient box, the gradient box is internally provided with three cavities with different heights, and the top ends of the three cavities are fixedly provided with storage boxes; the cooling boxes are arranged in the three different cavities respectively, a plurality of inner boxes are arranged in the three cooling boxes, and every two adjacent inner boxes are fixedly connected through a connecting rod; according to the device for comparing the variation characteristics of the phoenix tree root exudates and the rhizosphere microorganisms along the altitude gradients, the gradient box and the storage boxes at the top end of the gradient box are arranged, so that phoenix tree root samples with different altitude gradients can be visually displayed and compared; therefore, variation characteristics of nutglans regia root exudates and microorganisms at different altitude gradients can be better researched, and the nutglans regia culture box can be used as a culture box and can also be used as a sampling box for nutglans regia root systems on site at different altitudes.

Description

Technical Field

[0001] The invention relates to a comparison device, in particular to a comparison device for variation characteristics of root secretions of walnut and rhizosphere microorganisms along an altitude gradient. Background Art

[0002] The light walnut is a deciduous tree of the Rosaceae and Prunus genus. The young branches of the light walnut are green and gray-brown when old; the leaves are alternate, lanceolate or ovate-lanceolate, with acuminate tips; the leaf margins have rounded and blunt shallow serrations, and the entire margin near the top; the petiole is partially hairless, often with purple-red flat glands; its flowers are solitary and bloom before the leaves; the petals are broadly obovate, with slightly concave tips; the fruit is nearly spherical, fleshy, and does not crack; the flowering period is March-April, and the fruiting period is August-September. The fruit is nearly spherical, the core is ovate, flat and smooth, hence the name light walnut.

[0003] The light walnut grows in a semi-arid or semi-humid ecological environment. It has the characteristics of drought resistance, barrenness resistance, disease resistance, cold resistance, and long life. The light walnut likes light and is mostly distributed in sunny places such as farmland roadsides and forest edges with plenty of sunshine. The light walnut is generally propagated by seeds and cuttings.

[0004] In order to study the functional traits of absorbing roots and fungal infection characteristics, root exudates and rhizosphere microorganisms of Juglans regia at different altitudes in Linzhi and Shannan areas of the Qinghai-Tibet Plateau, combined with field investigations and indoor simulation experiments, the variation pattern of roots, rhizosphere microorganisms and root fungi along the altitude gradient was analyzed, and the response mechanism of root-microorganism interactions to high-altitude environmental stress was explored. It is now urgently necessary to design a device for comparing the variation characteristics of root exudates and rhizosphere microorganisms of Juglans regia along the altitude gradient. Summary of the invention

[0005] The purpose of the present invention is to provide a device for comparing the variation characteristics of root secretions and rhizosphere microorganisms of walnut along the altitude gradient, so as to solve the problems raised in the above-mentioned background technology.

[0006] To achieve the above object, the present invention provides the following technical solution: a device for comparing the variation characteristics of root secretions and rhizosphere microorganisms of walnut along the altitude gradient, comprising: A gradient box, wherein three cavities of different heights are arranged inside the gradient box, and storage boxes are fixedly installed on the tops of the three cavities; Cooling boxes are respectively placed in three different cavities, wherein a plurality of inner boxes are arranged inside the three cooling boxes, and two adjacent inner boxes are fixedly connected by a connecting rod; The tops of the three storage boxes are movably connected with cover plates, and the four corners of the tops of the three cover plates are interlaced with telescopic rods; A file drawer placed on one side of the gradient box and slidably connected to it.

[0007] As a preferred technical solution of the present invention, glass plates are fixedly provided on the front sides of the three storage boxes.

[0008] As a preferred technical solution of the present invention, the front sides of the three cooling boxes are respectively provided with label plates and handles.

[0009] As a preferred technical solution of the present invention, a freezing box is placed inside each of the plurality of inner boxes, and a freezing liquid is filled inside each of the plurality of freezing boxes.

[0010] As a preferred technical solution of the present invention, a temperature conduction plate is fixedly provided at the bottom end of the three storage boxes, inner grooves are opened on the inner walls on both sides of the three storage boxes, return pipes are embedded in the inner grooves, the backs of the three cooling boxes are fixedly connected with telescopic tubes, three micro water pumps are installed at equal intervals on the back of the gradient box, the input ends of the three micro water pumps are connected to one end of the corresponding telescopic tubes, the output ends of the three micro water pumps are fixedly connected with conduits, and one end of the three conduits is connected to the corresponding return pipes.

[0011] As a preferred technical solution of the present invention, the top end of the telescopic rod is fixedly connected with a vertical plate, and the middle part of the telescopic rod is fixedly provided with an anti-slip ring.

[0012] As a preferred technical solution of the present invention, a mounting plate is movably embedded on one side of the top of the cover plate, and a fill light is fixedly installed on the bottom end of the mounting plate.

[0013] As a preferred technical solution of the present invention, a light intensity tester, a humidity detector and a thermometer are fixedly installed in the middle of the top of the cover plate, and transparent plates are provided on the tops of the light intensity tester, humidity detector and thermometer.

[0014] As a preferred technical solution of the present invention, a folding telescopic box is fixedly installed on the other side of the top end of the cover plate, a strip box is fixedly installed on the other side of the bottom end of the cover plate, the strip box is connected to the folding telescopic box, and a plurality of nozzles are fixedly installed on the bottom end of the strip box.

[0015] Compared with the prior art, the present invention has the following beneficial effects: The present invention can intuitively display and compare the root samples of walnuts at different altitude gradients by arranging a gradient box and a plurality of storage boxes on the top of the gradient box, so as to better study the variation characteristics of the root secretions and microorganisms of walnuts at different altitude gradients. The present invention can be used as a laboratory incubator, and can also be used as a field sampling box for the root systems of walnuts at different altitudes, and can cooperate with a plurality of cooling boxes at the bottom. By built-in a plurality of freezing boxes, the low temperature condition can be transmitted to the storage box on the top, so as to simulate the low temperature environment at different altitudes. During the storage and transportation stage of the root samples of the walnuts, the environmental conditions at the current altitude can still be simulated, so as to greatly reduce the influence on the root secretions and microorganisms of the walnuts. The built-in plurality of cooling boxes can be flexibly disassembled and frozen, and can be used multiple times. When it is necessary to simulate the low temperature condition at high altitude, the micro-water pump and the conduit on the back can be used to pump cooling water into the inner tank, so as to realize more uniform temperature simulation. The label plate and the file drawer can be provided, so as to record the comparative research data. The data storage corresponds to the root samples of the walnuts at different altitudes, so that the research is more intuitive.

[0016] The present invention sets a cover plate on the top of the storage box, cooperates with four telescopic rods and a vertical plate, and can sample the bare walnut root system samples at different altitudes by extending the telescopic rod and erecting the cover plate on one side, so that the current altitude environmental conditions can be detected and recorded with the help of a light intensity tester, a humidity detector and a thermometer on the transparent plate. After the bare walnut root system samples are sampled and stored in the storage box, the freezing box can be adjusted to cool down according to the detection condition data, the humidity can be adjusted with the help of a foldable telescopic box, and the light intensity and temperature can be adjusted with the help of a fill light, so that the current internal environment of the storage box can be simulated into the current altitude environment, so as to maintain the initial state of the bare walnut root system secretions and microorganisms, so as to accurately carry out characteristic research.

[0017] The present invention can make the adjustment operation of various parameters more convenient and simple by arranging structures such as covers, drawers, and boxes. The structures can be used multiple times and can be used as simulation boxes or sampling boxes to study the root system of walnuts at different times. The functionality is stronger and the structure is compact, which is convenient for field transportation and carrying as well as laboratory research. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a front structural schematic diagram of the present invention; Figure 2 It is a schematic diagram of the cooling box structure of the present invention; Figure 3 It is a schematic diagram of the back structure of the present invention; Figure 4 It is a schematic diagram of the cover plate structure of the present invention; Figure 5 It is a schematic diagram of the internal structure of the present invention.

[0019] In the figure: 1. gradient box; 2. storage box; 3. glass plate; 4. document drawer; 5. cooling box; 6. label plate; 7. handle; 8. cover plate; 9. telescopic rod; 10. inner box; 11. connecting rod; 12. freezing box; 13. freezing liquid; 14. micro water pump; 15. telescopic tube; 16. catheter; 17. anti-slip ring; 18. vertical plate; 19. folding telescopic box; 20. strip box; 21. mounting plate; 22. fill light; 23. transparent plate; 24. light intensity tester; 25. humidity detector; 26. thermometer; 27. inner tank; 28. temperature conduction plate; 29. ​​reflux pipe. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] See also Figure 1-5 The present invention provides a technical solution for comparing the variation characteristics of walnut root secretions and rhizosphere microorganisms along the altitude gradient: according to Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 As shown, the comparative device for the variation characteristics of root exudates and rhizospheric microorganisms of walnut along the altitude gradient includes.

[0022] according to Figure 1 As shown, a gradient box 1 is provided with three cavities at different heights inside the gradient box 1, and storage boxes 2 are fixedly installed on the top of the three cavities. The three storage boxes 2 are used to simulate three different altitude environments to study the variation characteristics of root secretions and rhizosphere microorganisms of Walnut along the altitude gradient. The main research contents include (1) the variation characteristics of root traits and their correlations along the altitude gradient and their impact on the resource acquisition strategy of Walnut; (2) the variation characteristics of mycorrhizal fungal infection characteristics along the altitude gradient and their impact on the cold and drought resistance mechanism of Walnut; (3) the variation characteristics of root secretions and rhizosphere microorganisms along the altitude gradient and their impact on the cold and drought resistance mechanism of Walnut; (4) the adaptation mechanism of Walnut root-microorganism interaction to high altitude environment. Glass plates 3 are fixedly provided on the front of the three storage boxes 2 to facilitate the observation of the growth of Walnut. File drawers 4 are placed on one side of the gradient box 1 and are slidably connected to record various coefficients in different time periods inside different storage boxes 2. according to Figure 1 and Figure 2As shown, cooling boxes 5 are placed in three different cavities respectively. The front of the three cooling boxes 5 are respectively provided with label plates 6 and handles 7. The label plates 6 are convenient for distinguishing the three storage boxes 2. The label plates 6 can be written with a water-based pen. The light intensity tester 24, humidity detector 25 and thermometer 26 on the transparent plate 23 are used to detect the current altitude environmental conditions and record them on the label plate 6. After the sampling of the light walnut root system samples is completed and stored in the storage box 2, the data can be adjusted according to the detection conditions. Multiple inner boxes 10 are arranged inside the three cooling boxes 5. Two adjacent inner boxes 10 are fixedly connected by a connecting rod 11, and a plurality of inner boxes 10 are each placed with a freezing box 12, and a plurality of freezing boxes 12 are each filled with a freezing liquid 13. The freezing liquid 13 is a polymer gel, and its main components include sodium polyacrylate, sodium carboxymethyl cellulose (CMC), polyvinyl alcohol, etc. These components have strong water absorption and form a special gel state after mixing with water, which can maintain a low temperature for a long time in a frozen state. By freezing the freezing box 12, the low temperature is transferred to the inside of the corresponding storage box 2 after freezing, and then the low temperature environment at different altitudes is simulated.

[0023] according to Figure 3 and Figure 5 As shown, a heat conducting plate 28 is fixedly provided at the bottom end of the three storage boxes 2 to facilitate the low temperature environment inside the cooling box 5 to be transferred to the inside of the storage box 2. Inner grooves 27 are opened on the inner walls on both sides of the three storage boxes 2, and return pipes 29 are embedded in the multiple inner grooves 27. Telescopic tubes 15 are fixedly connected to the backs of the three cooling boxes 5. Three micro water pumps 14 are installed at equal intervals on the back of the gradient box 1. The input ends of the three micro water pumps 14 are connected to one end of the corresponding telescopic tubes 15. The output ends of the three micro water pumps 14 are fixedly connected to conduits 16. One end of the three conduits 16 is connected to the corresponding return pipes 29. Water can be added to the inside of the cooling box 5. After adding water, ice water is pumped from the conduit 16 into the inside of the return pipe 29 through the micro water pump 14, and then flows back to the inside of the cooling box 5 to form a cycle. On the one hand, water resources are saved, and on the other hand, the temperature inside the storage box 2 is better reduced to simulate the high-altitude low-temperature environment.

[0024] according to Figure 1 and Figure 4 As shown, the tops of the three storage boxes 2 are movably connected with cover plates 8, the four corners of the tops of the three cover plates 8 are interlaced with telescopic rods 9, the tops of the telescopic rods 9 are fixedly connected with vertical plates 18, and the middle of the telescopic rods 9 is fixedly provided with anti-slip rings 17 to prevent the telescopic rods 9 from falling into the interior of the three storage boxes 2; A mounting plate 21 is movably embedded on one side of the top of the cover plate 8, and a fill light 22 is fixedly installed at the bottom of the mounting plate 21, and the fill light 22 simulates light and temperature; a light intensity tester 24, a humidity detector 25 and a thermometer 26 are fixedly installed in the middle of the top of the cover plate 8, respectively, to detect and record the light intensity and temperature and humidity inside the corresponding storage box 2, and a transparent plate 23 is provided on the top of the light intensity tester 24, the humidity detector 25 and the thermometer 26; the light intensity tester 24 detects the light inside the storage box 2 In order to adjust the intensity and simulate the lighting conditions at different altitudes, a folding telescopic box 19 is fixedly installed on the other side of the top of the cover 8, and a strip box 20 is fixedly installed on the other side of the bottom of the cover 8. The strip box 20 is connected to the folding telescopic box 19, and a plurality of nozzles are fixedly installed on the bottom of the strip box 20. The interior of the folding telescopic box 19 is filled with water. By pressing the folding telescopic box 19, the water inside is sprayed out from the nozzle at the bottom of the strip box 20 to simulate rainfall. With the cooperation of the humidity detector 25, the internal humidity of the current storage box 2 can be accurately adjusted.

[0025] When used specifically, the device for comparing the variation characteristics of root secretions and rhizosphere microorganisms of walnuts along the altitude gradient of the present invention first pulls up the telescopic rod 9 to remove the corresponding cover plate 8, and then plants three identical specimens of walnuts in the corresponding storage box 2, and then puts the frozen freezing box 12 into the inner box 10, and then injects water into the cooling box 5 to cool the water, and the heat conducting plate 28 transfers the low temperature inside the cooling box 5 to the corresponding storage box 2, and then drives the micro water pump 14 to pump cold water out of the cooling box 5, and enters the conduit 16 through the telescopic tube 15, and then connects to the inside of the reflux pipe 29 to cool the inner wall of the storage box 2, thereby simulating the low temperature environment at different altitudes, and then drives the fill light 22, and the fill light 22 supplements the light and temperature inside the storage box 2, and the inside of the foldable telescopic box 19 is folded. The foldable telescopic box 19 is filled with water, and the water inside is sprayed out from the nozzle at the bottom of the strip box 20 by pressing the foldable telescopic box 19 to simulate rainfall. Then, the light intensity tester 24, humidity detector 25 and thermometer 26 are used to detect and record the light intensity and temperature and humidity inside the storage box 2. When adjusting the above environmental parameters to simulate environmental conditions at different altitudes, it can be used as a laboratory incubator or as a field sampling box for the roots of bare walnuts at different altitudes. When sampling in the field, the environmental conditions at different altitudes can be detected with the help of multiple functional instruments on the cover 8. Then, the cold water adjustment, water spray adjustment and light adjustment at the bottom of the storage box 2 are used to simulate the environmental conditions at different altitudes, so as to carry out subsequent laboratory transfer research. The root secretions and microorganisms of the bare walnuts will not be affected by environmental changes during transportation and research.

[0026] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A device for comparing the variation characteristics of root secretions and rhizosphere microorganisms of walnut along the altitude gradient, characterized in that: include: A gradient box (1), wherein three cavities of different heights are arranged inside the gradient box (1), and storage boxes (2) are fixedly mounted on the tops of the three cavities; Cooling boxes (5) are respectively placed inside three different cavities, a plurality of inner boxes (10) are arranged inside each of the three cooling boxes (5), and two adjacent inner boxes (10) are fixedly connected via a connecting rod (11); The top ends of the three storage boxes (2) are all movably connected to a cover plate (8), and the four corners of the top ends of the three cover plates (8) are all interlaced and connected to telescopic rods (9); A file drawer (4) is placed on one side of the gradient box (1) and is slidably connected thereto.

2. The device for comparing the variation characteristics of root secretions and rhizosphere microorganisms of Walnut along the altitude gradient according to claim 1, characterized in that: Glass plates (3) are fixedly provided on the front sides of the three storage boxes (2).

3. The device for comparing the variation characteristics of the root secretions of Walnut glauca and rhizosphere microorganisms along the altitude gradient according to claim 1, characterized in that: The front sides of the three cooling boxes (5) are respectively provided with a label plate (6) and a handle (7).

4. The device for comparing the variation characteristics of root secretions and rhizosphere microorganisms of Walnut along the altitude gradient according to claim 1, characterized in that: A freezing box (12) is placed inside each of the plurality of inner boxes (10), and a freezing liquid (13) is filled inside each of the plurality of freezing boxes (12).

5. The device for comparing the variation characteristics of root secretions and rhizosphere microorganisms of Walnut along the altitude gradient according to claim 1, characterized in that: A temperature conducting plate (28) is fixedly provided at the bottom of each of the three storage boxes (2), inner grooves (27) are provided on the inner walls on both sides of each of the three storage boxes (2), return pipes (29) are embedded in each of the plurality of inner grooves (27), the backs of each of the three cooling boxes (5) are fixedly connected to a telescopic tube (15), three micro water pumps (14) are evenly spacedly installed on the back of the gradient box (1), the input ends of each of the three micro water pumps (14) are connected to one end of the corresponding telescopic tube (15), the output ends of each of the three micro water pumps (14) are fixedly connected to a conduit (16), and one end of each of the three conduits (16) is connected to the corresponding return pipe (29).

6. The device for comparing the variation characteristics of root secretions and rhizosphere microorganisms of Walnut along the altitude gradient according to claim 1, characterized in that: The top end of the telescopic rod (9) is fixedly connected to a vertical plate (18), and the middle portion of the telescopic rod (9) is fixedly provided with an anti-slip ring (17).

7. The device for comparing the variation characteristics of root secretions and rhizosphere microorganisms of Walnut along the altitude gradient according to claim 1, characterized in that: A mounting plate (21) is movably embedded on one side of the top end of the cover plate (8), and a fill light (22) is fixedly mounted on the bottom end of the mounting plate (21).

8. The device for comparing the variation characteristics of root secretions and rhizosphere microorganisms of Walnut along the altitude gradient according to claim 1, characterized in that: A light intensity tester (24), a humidity detector (25) and a thermometer (26) are respectively fixedly mounted in the middle of the top of the cover plate (8), and a transparent plate (23) is provided at the top of the light intensity tester (24), the humidity detector (25) and the thermometer (26).

9. The device for comparing the variation characteristics of root secretions and rhizosphere microorganisms of Walnut along the altitude gradient according to claim 1, characterized in that: A folding telescopic box (19) is fixedly mounted on the other side of the top end of the cover plate (8), and a strip box (20) is fixedly mounted on the other side of the bottom end of the cover plate (8); the strip box (20) is connected to the folding telescopic box (19), and a plurality of nozzles are fixedly mounted on the bottom end of the strip box (20).

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