Selenium-rich liquid fertilizer and planting method of selenium-rich coriander

Abstract

The invention discloses a selenium-rich liquid fertilizer and a selenium-rich coriander planting method, and belongs to the technical field of selenium-rich crop planting, and the selenium-rich liquid fertilizer comprises a basic selenium-rich liquid fertilizer and a growth regulator. The selenium-rich basic liquid fertilizer and the growth regulator are cooperatively used and are sprayed in a specific growth period, so that the selenium content, the reducing sugar content, the total flavone content, the hydroxyl radical scavenging rate and other indexes in the coriander are remarkably improved, and the coriander is rich in nutritional ingredients and remarkable in selenium content. In addition, the growth regulator and the selenium-rich liquid fertilizer provide a new thought for planting of aromatic crops including coriander.

Description

Technical Field

[0001] The invention belongs to the technical field of selenium-rich crop planting, and particularly relates to a selenium-rich liquid fertilizer and a planting method for selenium-rich coriander. Background Art

[0002] Coriander is another name for many plants, such as cilantro, eryngium, essambar, and dense-flowered essambar. Coriander is also known as salt coriander, cilantro, cilantro, cilantro, salt beard, and starry sky. It is an annual herb of the genus Coriander in the family Apiaceae. The tender stems and fresh leaves of coriander have a special fragrance and are often used as a garnish and flavor enhancer for dishes. Coriander contains rich nutritional active ingredients and has good edible and medicinal value such as antibacterial, antioxidant, anti-anxiety, and anti-cancer effects.

[0003] Selenium is a non-metallic chemical element in the form of gray or red crystals or red amorphous powder. It is an essential trace element for the health of the body. It has important biological functions in anti-oxidation and anti-aging, lowering blood pressure, regulating immunity, preventing cancer and anti-atherosclerosis, etc. It is also a component of some enzymes, including glutathione peroxidase, deiodinase iodothyronine and thioredoxin reductase. Selenium can protect cells from the harmful effects of free radicals formed during oxidation. Selenium in the human body mainly comes from plants and animals, among which organic selenium in plants is more easily absorbed and utilized by the human body than organic selenium in animals. Therefore, organic selenium in plants is one of the main sources of selenium supplementation for the human body. With the development of the times, more and more people pay attention to their health and realize the importance of selenium supplementation. Increasing the selenium content of plants through various organic transformation methods of selenium is the safest and most effective way to increase the selenium intake of humans and animals.

[0004] At present, the production of selenium-enriched coriander is mainly carried out by adding a certain amount of sodium selenite to farmyard manure as a base fertilizer. However, due to the influence of the physical and chemical properties of the planting soil, the selenium content of coriander produced by this method is unstable and very low. In addition, directly applying sodium selenite to the soil is very likely to cause soil selenium pollution, and the absorption and utilization efficiency of selenium by plants is also low, so it is an environmentally unfriendly selenium-enriched plant planting technology.

[0005] Based on this, we proposed a selenium-rich liquid fertilizer and a method for growing selenium-rich coriander, hoping to solve the shortcomings of the existing technology. Summary of the invention

[0006] The invention aims to solve the existing problems and provides a selenium-enriched liquid fertilizer and a method for planting selenium-enriched coriander.

[0007] The present invention is achieved through the following technical solutions:

[0008] A selenium-rich liquid fertilizer comprises a basic selenium-rich liquid fertilizer and a growth regulator.

[0009] Furthermore, the preparation of the basic selenium-rich liquid fertilizer comprises the following steps:

[0010] (1) drying corn cobs, rice straws, purple sweet potato vines, and kudzu vines to constant weight, respectively, and crushing them in a plant grinder in a mass ratio of corn cobs: rice straws: purple sweet potato vines: kudzu vines = 1:2-3:0.5-0.7:0.3-0.5 to obtain a mixed powder for later use;

[0011] (2) immersing the obtained mixed powder in a treatment agent, subjecting the mixed powder to constant temperature ultrasonic stirring for 3 to 5 hours, and then placing the mixed powder in a high temperature and high pressure cooker for high temperature and high pressure treatment, followed by filtering, collecting the filtrate and concentrating it to 40 to 60% of the original volume to obtain a crop concentrate for later use;

[0012] (3) After the crop concentrate and sterile water are mixed at a volume ratio of 1:8-12, manganese sulfate, magnesium sulfate and sodium chloride are added, stirred and mixed, and then sterilized. After cooling to room temperature, Bacillus subtilis is inoculated, and Na2SeO3 is added after culturing in a shaking incubator for 3-5 hours. The culture is continued for 2-4 days, and then filtered to obtain a basic selenium-rich liquid fertilizer.

[0013] Furthermore, the components and corresponding weight percentages of the treatment agent described in step (2) are: 0.7-1.3% fulvic acid, 1-2% lanthanum nitrate, 0.8-1.4% cerium nitrate, 7-10% phosphate amphoteric surfactant, and the balance is pure water.

[0014] Furthermore, the constant temperature in step (2) is 50-70°C.

[0015] Furthermore, the high temperature and high pressure treatment in step (2) is carried out at a temperature of 100 to 120° C. and a pressure of 0.2 to 0.5 MPa.

[0016] Furthermore, the final concentrations of manganese sulfate, magnesium sulfate and sodium chloride in step (3) are 0.02-0.03 mg / mL, 0.1-0.2 mg / mL and 0.1-0.3 mg / mL, respectively.

[0017] Furthermore, the inoculation amount of Bacillus subtilis described in step (3) is 2-3%;

[0018] The culture temperature is 35-39°C and the shaking speed is 120-150 rpm.

[0019] Furthermore, the preparation method of the growth regulator is as follows: adding brown algae oligosaccharides to deionized water under ultrasonic conditions, ultrasonically stirring at 60-70°C for 30-50 minutes, adding glycidyl trimethylammonium chloride while stirring, fully stirring and mixing, heating to 80-90°C for reaction for 20-30 minutes, cooling to 50-60°C, adding deionized water and amino acid chelate, stirring at a constant temperature for 30-40 minutes, cooling to room temperature, and then adding glutathione and silk fibroin, and continuing to stir for 1-2 hours.

[0020] Furthermore, in 1L of the growth regulator, the added amounts of each raw material are: 0.6-1g of brown algae oligosaccharide, 1-1.5g of glycidyl trimethylammonium chloride, 0.8-1.2g of amino acid chelate, 2-3g of glutathione, and 8-12g of silk fibroin.

[0021] A method for planting selenium-enriched coriander comprises the following steps:

[0022] (1) Apply basal fertilizer to the soil before planting;

[0023] (2) 4 to 5 days after the coriander seedlings emerge, apply the above-mentioned basic selenium-rich liquid fertilizer to the roots;

[0024] (3) When the coriander seedlings grow to a height of 8 to 12 cm, mix the above-mentioned basic selenium-rich liquid fertilizer and growth regulator in a ratio of 70 to 90:1 and spray them on the coriander leaves.

[0025] Compared with the prior art, the present invention has the following advantages:

[0026] The invention provides a selenium-rich liquid fertilizer and a method for planting selenium-rich coriander. The selenium-rich basic liquid fertilizer and a growth regulator are used in combination and sprayed in a specific growth period, so that the selenium content, reducing sugar content, total flavonoids content, hydroxyl free radical scavenging rate and other indicators in the coriander are significantly improved. The coriander is rich in nutrients and has a significant selenium content.

[0027] First, since the main way for plants to absorb selenium is through the root system, mainly through the root hairs to increase the contact area with the soil, thereby improving the absorption efficiency of selenium, after selenium is absorbed into plant cells, it needs to be transported to organelles such as chloroplasts for biological transformation, and the root absorption rate is faster than the leaf surface transformation rate. In addition, selenium may affect the utilization efficiency and toxicity of selenium by plants during the transformation process. Therefore, in order to improve the effective utilization rate of selenium, this application adjusts the fertilization method, according to the growth characteristics of coriander, and adopts different fertilization methods at different growth stages, thereby increasing the selenium content and improving the bioavailability of selenium. Meet people's demand for selenium and increase the added value of coriander.

[0028] Secondly, corn cobs, rice straw, purple sweet potato vines, and kudzu vines are treated in a series of ways according to a certain ratio, sterilized after adding manganese sulfate, magnesium sulfate, and sodium chloride, and then inoculated with Bacillus subtilis, and then Na2SeO3 is added after culturing in a shaking table for 3 to 5 hours. The rare earth compound is used in combination with nutrients to intensify the logarithmic growth of Bacillus subtilis, promote metabolism, shorten the stable period, and absorb and utilize selenium during the growth process of Bacillus subtilis, and convert it into organic selenium through a series of organic combinations (with proteins, polysaccharides, etc.) in the body, and metabolize inorganic selenium compounds with low bioavailability into organic selenium forms such as selenocysteine ​​or selenomethionine with rich bioavailability, and the conversion rate of selenium is very high. In addition, the basic selenium-rich liquid fertilizer of the present application can improve the permeability of cells, thereby improving the absorption and conversion of effective ingredients by the root system. In growth regulation, brown algae oligosaccharides, glycidyl trimethylammonium chloride, amino acid chelates, glutathione, silk fibroin and basic selenium-rich liquid fertilizer cooperate with each other. On the one hand, they can increase the adhesion ability of liquid fertilizer on the leaf surface, prolong the action time of liquid fertilizer and enhance its effect; on the other hand, the addition of growth regulators can stimulate the leaves, change the activity of enzyme genes such as ATP sulfurylase (APS) and selenocysteine ​​methyltransferase (SMT) in the metabolism of selenium in plants, promote the transformation of selenium in chloroplasts, and promote the absorption and transformation of active ingredients in liquid fertilizer by leaves, thereby achieving the enrichment of organic selenium without toxicity to plants.

[0029] Finally, the growth regulator and selenium-enriched liquid fertilizer of the present application provide new ideas for the cultivation of spice crops including coriander. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 The effect of the change of the amount of selenium-rich liquid fertilizer applied to the root base on the selenium content in coriander;

[0031] Figure 2 This study is about the effect of changing the amount of selenium-rich liquid fertilizer sprayed on the leaves on the selenium content in coriander. DETAILED DESCRIPTION

[0032] In order to further explain the present invention, it is described below in conjunction with the following specific embodiments.

[0033] Example 1

[0034] A method for planting selenium-enriched coriander comprises the following steps:

[0035] (1) Apply basal fertilizer to the soil before planting;

[0036] (2) 4 days after the coriander seedlings emerge, apply the above-mentioned basic selenium-rich liquid fertilizer to the roots;

[0037] (3) When the coriander seedlings grow to 8 cm in height, mix the above-mentioned basic selenium-rich liquid fertilizer and growth regulator in a ratio of 70:1 and spray them on the coriander leaves.

[0038] The base fertilizer is decomposed organic fertilizer, and the fertilizer application rate is 800 kg / mu;

[0039] The application amount of the basic selenium-rich liquid fertilizer is 40L / mu;

[0040] The spraying amount during foliar spraying is 20L / mu;

[0041] The preparation of the basic selenium-rich liquid fertilizer comprises the following steps:

[0042] (1) drying corn cobs, rice straws, purple sweet potato vines, and kudzu vines to constant weight, respectively, and crushing them in a plant grinder in a mass ratio of corn cobs: rice straws: purple sweet potato vines: kudzu vines = 1:2:0.5:0.3 to obtain a mixed powder for later use;

[0043] (2) immersing the obtained mixed powder in a treatment agent, treating it with constant temperature ultrasonic stirring at 50° C. for 3 h, and then placing it in a high temperature and high pressure cooker at 100° C. and 0.2 MPa, and then filtering, collecting the filtrate and concentrating it to 40% of the original volume to obtain a crop concentrate for use;

[0044] The components and corresponding weight percentages in the treatment agent are: 0.7% fulvic acid, 1% lanthanum nitrate, 0.8% cerium nitrate, 7% phosphate amphoteric surfactant, and the balance is pure water;

[0045] (3) After the crop concentrate and sterile water are mixed at a volume ratio of 1:8, manganese sulfate, magnesium sulfate, and sodium chloride are added, stirred and mixed, and then sterilized. After cooling to room temperature, Bacillus subtilis is inoculated, and the inoculation amount of Bacillus subtilis is 2%. After culturing at 35° C. and 120 rpm for 3 h in a shaker, Na2SeO3 is added, and the culture is continued for 2 days, and then filtered to obtain a basic selenium-rich liquid fertilizer;

[0046] The final concentrations of manganese sulfate, magnesium sulfate, and sodium chloride are 0.02 mg / mL, 0.1 mg / mL, and 0.1 mg / mL, respectively;

[0047] The preparation method of the growth regulator is as follows: adding brown algae oligosaccharide to deionized water under ultrasonic conditions, ultrasonically stirring at 60° C. for 30 minutes, adding glycidyl trimethylammonium chloride while stirring, fully stirring and mixing, heating to 80° C. for reaction for 20 minutes, cooling to 50° C., adding deionized water and amino acid chelate, stirring at a constant temperature for 30 minutes, cooling to room temperature, and then adding glutathione and silk fibroin, and continuing to stir for 1 hour;

[0048] In 1L of the growth regulator, the added amounts of the raw materials are: 0.6g of brown algae oligosaccharide, 1g of glycidyl trimethylammonium chloride, 0.8g of amino acid chelate, 2-3g of glutathione, and 8g of silk fibroin.

[0049] Example 2

[0050] A method for planting selenium-enriched coriander comprises the following steps:

[0051] (1) Apply basal fertilizer to the soil before planting;

[0052] (2) 4 days after the coriander seedlings emerge, apply the above-mentioned basic selenium-rich liquid fertilizer to the roots;

[0053] (3) When the coriander seedlings grow to a height of 10 cm, mix the above-mentioned basic selenium-rich liquid fertilizer and growth regulator in a ratio of 80:1 and spray them on the coriander leaves.

[0054] The base fertilizer is decomposed organic fertilizer, and the fertilizer application rate is 800 kg / mu;

[0055] The application amount of the basic selenium-rich liquid fertilizer is 45L / mu;

[0056] The spraying amount during foliar spraying is 30L / mu;

[0057] The preparation of the basic selenium-rich liquid fertilizer comprises the following steps:

[0058] (1) drying corn cobs, rice straws, purple sweet potato vines, and kudzu vines to constant weight, respectively, and crushing them in a plant grinder in a mass ratio of corn cobs: rice straws: purple sweet potato vines: kudzu vines = 1:2.5:0.6:0.4 to obtain a mixed powder for later use;

[0059] (2) immersing the obtained mixed powder in a treatment agent, treating it with constant temperature ultrasonic stirring at 60° C. for 4 h, and then placing it in a high temperature and high pressure cooker at 110° C. and 0.35 MPa, and then filtering, collecting the filtrate and concentrating it to 50% of the original volume to obtain a crop concentrate for use;

[0060] The components and corresponding weight percentages in the treatment agent are: 1% fulvic acid, 1.5% lanthanum nitrate, 1.1% cerium nitrate, 8% phosphate amphoteric surfactant, and the balance is pure water;

[0061] (3) After the crop concentrate and sterile water are mixed at a volume ratio of 1:10, manganese sulfate, magnesium sulfate, and sodium chloride are added, stirred and mixed, and then sterilized. After cooling to room temperature, Bacillus subtilis is inoculated, and the inoculation amount of Bacillus subtilis is 2.5%. After culturing at 37° C. and 130 rpm for 4 hours in a shaker, Na2SeO3 is added, and the culture is continued for 3 days, and then filtered to obtain a basic selenium-rich liquid fertilizer;

[0062] The final concentrations of manganese sulfate, magnesium sulfate, and sodium chloride are 0.025 mg / mL, 0.15 mg / mL, and 0.2 mg / mL, respectively;

[0063] The preparation method of the growth regulator is as follows: adding brown algae oligosaccharide to deionized water under ultrasonic conditions, ultrasonically stirring at 65° C. for 40 minutes, adding glycidyl trimethylammonium chloride while stirring, fully stirring and mixing, heating to 85° C. for reaction for 25 minutes, cooling to 55° C., adding deionized water and amino acid chelate, stirring at a constant temperature for 35 minutes, cooling to room temperature, and then adding glutathione and silk fibroin, and continuing to stir for 1.5 hours;

[0064] In 1L of the growth regulator, the added amounts of the raw materials are: 0.8g of brown algae oligosaccharide, 1.2g of glycidyl trimethylammonium chloride, 1g of amino acid chelate, 2.5g of glutathione, and 10g of silk fibroin.

[0065] Example 3

[0066] A method for planting selenium-enriched coriander comprises the following steps:

[0067] (1) Apply basal fertilizer to the soil before planting;

[0068] (2) 5 days after the coriander seedlings emerge, apply the above-mentioned basic selenium-rich liquid fertilizer to the roots;

[0069] (3) When the coriander seedlings grow to a height of 12 cm, mix the above-mentioned basic selenium-rich liquid fertilizer and growth regulator in a ratio of 90:1 and spray them on the coriander leaves.

[0070] The base fertilizer is decomposed organic fertilizer, and the fertilizer application rate is 800 kg / mu;

[0071] The application amount of the basic selenium-rich liquid fertilizer is 50L / mu;

[0072] The spraying amount during foliar spraying is 40L / mu;

[0073] The preparation of the basic selenium-rich liquid fertilizer comprises the following steps:

[0074] (1) drying corn cobs, rice straws, purple sweet potato vines, and kudzu vines to constant weight, respectively, and crushing them in a plant grinder in a mass ratio of corn cobs: rice straws: purple sweet potato vines: kudzu vines = 1:3:0.7:0.5 to obtain a mixed powder for later use;

[0075] (2) immersing the obtained mixed powder in a treatment agent, treating it with constant temperature ultrasonic stirring at 70° C. for 5 h, and then placing it in a high temperature and high pressure cooker at 120° C. and 0.5 MPa, and then filtering, collecting the filtrate and concentrating it to 60% of the original volume to obtain a crop concentrate for use;

[0076] The components and corresponding weight percentages in the treatment agent are: 1.3% fulvic acid, 2% lanthanum nitrate, 1.4% cerium nitrate, 10% phosphate amphoteric surfactant, and the balance is pure water;

[0077] (3) After the crop concentrate and sterile water are mixed at a volume ratio of 1:12, manganese sulfate, magnesium sulfate, and sodium chloride are added, stirred and mixed, and then sterilized. After cooling to room temperature, Bacillus subtilis is inoculated, and the inoculation amount of Bacillus subtilis is 3%. After culturing at 39° C. and 150 rpm for 5 hours in a shaker, Na2SeO3 is added, and the culture is continued for 4 days, and then filtered to obtain a basic selenium-rich liquid fertilizer;

[0078] The final concentrations of manganese sulfate, magnesium sulfate, and sodium chloride are 0.03 mg / mL, 0.2 mg / mL, and 0.3 mg / mL, respectively;

[0079] The preparation method of the growth regulator is as follows: adding brown algae oligosaccharide to deionized water under ultrasonic conditions, ultrasonically stirring at 70° C. for 50 minutes, adding glycidyl trimethylammonium chloride while stirring, fully stirring and mixing, heating to 90° C. for reaction for 30 minutes, cooling to 60° C., adding deionized water and amino acid chelate, stirring at a constant temperature for 40 minutes, cooling to room temperature, and then adding glutathione and silk fibroin, and continuing to stir for 2 hours;

[0080] In 1L of the growth regulator, the added amounts of the raw materials are: 1g of brown algae oligosaccharide, 1.5g of glycidyl trimethylammonium chloride, 1.2g of amino acid chelate, 3g of glutathione, and 12g of silk fibroin.

[0081] Comparative Example 1

[0082] A method for planting selenium-enriched coriander comprises the following steps:

[0083] (1) Apply basal fertilizer to the soil before planting;

[0084] (2) 4 days after the coriander seedlings emerge, apply the above-mentioned basic selenium-rich liquid fertilizer to the roots;

[0085] (3) When the coriander seedlings grow to a height of 10 cm, spray the coriander leaves with the above-mentioned basic selenium-rich liquid fertilizer.

[0086] The base fertilizer is decomposed organic fertilizer, and the fertilizer application rate is 800 kg / mu;

[0087] The application amount of the basic selenium-rich liquid fertilizer is 45L / mu;

[0088] The spraying amount during foliar spraying is 30L / mu;

[0089] The preparation of the basic selenium-rich liquid fertilizer comprises the following steps:

[0090] (1) drying corn cobs, rice straws, purple sweet potato vines, and kudzu vines to constant weight, respectively, and crushing them in a plant grinder in a mass ratio of corn cobs: rice straws: purple sweet potato vines: kudzu vines = 1:2.5:0.6:0.4 to obtain a mixed powder for later use;

[0091] (2) immersing the obtained mixed powder in a treatment agent, treating it with constant temperature ultrasonic stirring at 60° C. for 4 h, and then placing it in a high temperature and high pressure cooker at 110° C. and 0.35 MPa, and then filtering, collecting the filtrate and concentrating it to 50% of the original volume to obtain a crop concentrate for use;

[0092] The components and corresponding weight percentages in the treatment agent are: 1% fulvic acid, 1.5% lanthanum nitrate, 1.1% cerium nitrate, 8% phosphate amphoteric surfactant, and the balance is pure water;

[0093] (3) After the crop concentrate and sterile water are mixed at a volume ratio of 1:10, manganese sulfate, magnesium sulfate, and sodium chloride are added, stirred and mixed, and then sterilized. After cooling to room temperature, Bacillus subtilis is inoculated, and the inoculation amount of Bacillus subtilis is 2.5%. After culturing at 37° C. and 130 rpm for 4 hours in a shaker, Na2SeO3 is added, and the culture is continued for 3 days, and then filtered to obtain a basic selenium-rich liquid fertilizer;

[0094] The final concentrations of manganese sulfate, magnesium sulfate, and sodium chloride are 0.025 mg / mL, 0.15 mg / mL, and 0.2 mg / mL, respectively;

[0095] Comparative Example 2

[0096] A method for planting selenium-enriched coriander comprises the following steps:

[0097] (1) Apply basal fertilizer to the soil before planting;

[0098] (2) 4 days after the coriander seedlings emerge, mix the above-mentioned basic selenium-rich liquid fertilizer and growth regulator in a ratio of 80:1 and apply to the roots;

[0099] (3) When the coriander seedlings grow to a height of 10 cm, mix the above-mentioned basic selenium-rich liquid fertilizer and growth regulator in a ratio of 80:1 and spray them on the coriander leaves.

[0100] The base fertilizer is decomposed organic fertilizer, and the fertilizer application rate is 800 kg / mu;

[0101] The application amount of the basic selenium-rich liquid fertilizer is 45L / mu;

[0102] The spraying amount during foliar spraying is 30L / mu;

[0103] The preparation of the basic selenium-rich liquid fertilizer comprises the following steps:

[0104] (1) drying corn cobs, rice straws, purple sweet potato vines, and kudzu vines to constant weight, respectively, and crushing them in a plant grinder in a mass ratio of corn cobs: rice straws: purple sweet potato vines: kudzu vines = 1:2.5:0.6:0.4 to obtain a mixed powder for later use;

[0105] (2) immersing the obtained mixed powder in a treatment agent, treating it with constant temperature ultrasonic stirring at 60° C. for 4 h, and then placing it in a high temperature and high pressure cooker at 110° C. and 0.35 MPa, and then filtering, collecting the filtrate and concentrating it to 50% of the original volume to obtain a crop concentrate for use;

[0106] The components and corresponding weight percentages in the treatment agent are: 1% fulvic acid, 1.5% lanthanum nitrate, 1.1% cerium nitrate, 8% phosphate amphoteric surfactant, and the balance is pure water;

[0107] (3) After the crop concentrate and sterile water are mixed at a volume ratio of 1:10, manganese sulfate, magnesium sulfate, and sodium chloride are added, stirred and mixed, and then sterilized. After cooling to room temperature, Bacillus subtilis is inoculated, and the inoculation amount of Bacillus subtilis is 2.5%. After culturing at 37° C. and 130 rpm for 4 hours in a shaker, Na2SeO3 is added, and the culture is continued for 3 days, and then filtered to obtain a basic selenium-rich liquid fertilizer;

[0108] The final concentrations of manganese sulfate, magnesium sulfate, and sodium chloride are 0.025 mg / mL, 0.15 mg / mL, and 0.2 mg / mL, respectively;

[0109] The preparation method of the growth regulator is as follows: adding brown algae oligosaccharide to deionized water under ultrasonic conditions, ultrasonically stirring at 65° C. for 40 minutes, adding glycidyl trimethylammonium chloride while stirring, fully stirring and mixing, heating to 85° C. for reaction for 25 minutes, cooling to 55° C., adding deionized water and amino acid chelate, stirring at a constant temperature for 35 minutes, cooling to room temperature, then adding glutathione and silk fibroin, and continuing to stir for 1.5 hours;

[0110] In 1L of the growth regulator, the added amounts of the raw materials are: 0.8g of brown algae oligosaccharide, 1.2g of glycidyl trimethylammonium chloride, 1g of amino acid chelate, 2.5g of glutathione, and 10g of silk fibroin.

[0111] Comparative Example 3

[0112] A method for planting selenium-enriched coriander comprises the following steps:

[0113] (1) Apply well-rotted organic fertilizer to the soil before planting;

[0114] (2) Four days after the coriander seedlings emerged, the above-mentioned decomposed organic fertilizer was used for root fertilization.

[0115] The amount of fertilizer applied before planting is 800 kg / mu; the amount of fertilizer applied at the root is 45L / mu;

[0116] The preparation of the basic selenium-rich liquid fertilizer comprises the following steps:

[0117] (1) drying corn cobs, rice straws, purple sweet potato vines, and kudzu vines to constant weight, respectively, and crushing them in a plant grinder in a mass ratio of corn cobs: rice straws: purple sweet potato vines: kudzu vines = 1:2.5:0.6:0.4 to obtain a mixed powder for later use;

[0118] (2) immersing the obtained mixed powder in a treatment agent, treating it with constant temperature ultrasonic stirring at 60° C. for 4 h, and then placing it in a high temperature and high pressure cooker at 110° C. and 0.35 MPa, and then filtering, collecting the filtrate and concentrating it to 50% of the original volume to obtain a crop concentrate for use;

[0119] The components and corresponding weight percentages in the treatment agent are: 1% fulvic acid, 1.5% lanthanum nitrate, 1.1% cerium nitrate, 8% phosphate amphoteric surfactant, and the balance is pure water;

[0120] (3) After the crop concentrate and sterile water are mixed at a volume ratio of 1:10, manganese sulfate, magnesium sulfate, and sodium chloride are added, stirred and mixed, and then sterilized. After cooling to room temperature, Bacillus subtilis is inoculated, and the inoculation amount of Bacillus subtilis is 2.5%. After culturing at 37° C. and 130 rpm for 4 hours in a shaker, Na2SeO3 is added, and the culture is continued for 3 days, and then filtered to obtain a basic selenium-rich liquid fertilizer;

[0121] The final concentrations of manganese sulfate, magnesium sulfate, and sodium chloride are 0.025 mg / mL, 0.15 mg / mL, and 0.2 mg / mL, respectively;

[0122] The preparation method of the growth regulator is as follows: adding brown algae oligosaccharide to deionized water under ultrasonic conditions, ultrasonically stirring at 65° C. for 40 minutes, adding glycidyl trimethylammonium chloride while stirring, fully stirring and mixing, heating to 85° C. for reaction for 25 minutes, cooling to 55° C., adding deionized water and amino acid chelate, stirring at a constant temperature for 35 minutes, cooling to room temperature, then adding glutathione and silk fibroin, and continuing to stir for 1.5 hours;

[0123] In 1L of the growth regulator, the added amounts of the raw materials are: 0.8g of brown algae oligosaccharide, 1.2g of glycidyl trimethylammonium chloride, 1g of amino acid chelate, 2.5g of glutathione, and 10g of silk fibroin.

[0124] test

[0125] The coriander samples of Examples 1 to 3 and Comparative Examples 1 to 3 were cleaned with ultrapure water, dried in an oven to constant weight, and then crushed with a plant grinder and passed through a 200-mesh sieve for standby use.

[0126] 1. Determination of selenium content

[0127] Weigh 0.5g of powder sample on an electronic balance and place it in a 50mL flask. Add 15mL of mixed acid (HNO3: HClO4 is 2:1) and digest for 24h. Heat on a hot plate until the sample solution is about 1mL, remove and cool, then add 10mL of concentrated HCl, heat again until the sample solution is about 1mL, remove and cool. Add a small amount of ultrapure water to the digestion solution, sonicate in a sonicator until the solution is clear, transfer to a 50mL volumetric flask, add 12.5mL of concentrated HCl, and then dilute to the mark with ultrapure water for testing.

[0128] 2. Determination of reducing sugar content

[0129] Determined according to the first method - direct titration method in GB 5009.7-2016 "National Food Safety Standard Determination of Reducing Sugar in Food".

[0130] 3. Determination of protein content

[0131] The protein content in dried coriander products was determined by the Coomassie brilliant blue method.

[0132] 4. Determination of vitamin C content

[0133] The determination was carried out according to GB / T 6195-1986 “Determination of vitamin C content in fruits and vegetables (2,6-dichloroindophenol titration method)”.

[0134] 5. Determination of total flavonoids content

[0135] The determination was carried out according to the method in DB43 / T 476-2009 “Determination of total flavonoids in plant-derived foods”.

[0136] 6. Determination of Hydroxyl Radical Scavenging Capacity

[0137] Use the hydroxyl free radical determination kit and an ultraviolet spectrophotometer to accurately determine the hydroxyl free radical at a wavelength of 550nm in strict accordance with the operating procedures in the instructions.

[0138] The test results are shown in Table 1 below.

[0139] Table 1

[0140]

[0141] It can be seen from the experimental data in Table 1 above that the coriander grown by the methods of Examples 1 to 3 are superior to the control group in terms of selenium content, reducing sugar content, total flavonoids content and hydroxyl radical scavenging rate. The coriander is rich in nutrients and has a significant selenium content.

[0142] Comparison of Comparative Example 1 with Example 2 shows that the addition of growth regulators during the last step of foliar spraying can increase fertilizer efficiency and contribute to the accumulation of nutrient components.

[0143] From the comparison between Comparative Example 2 and Example 2, it can be seen that the addition of growth regulators during root application in step (2) has no significant effect on the content of nutrients, and may even reduce the content of some nutrients, indicating that the time of adding growth regulators is very important, and adding them at the right time has a significant effect on the content of nutrients in coriander.

[0144] On the basis of the above experiment, with Example 2 as the benchmark, the application amount of the basic selenium-rich liquid fertilizer in step (2) and the foliar spraying amount in step (3) were changed respectively, and the change in selenium content with the increase in the application amount was measured.

[0145] The results are as follows Figure 1 and Figure 2 shown.

[0146] Depend on Figure 1 It can be seen that after the coriander grows to a height of 10 cm, the above-mentioned basic selenium-rich liquid fertilizer and growth regulator are mixed in a ratio of 80:1 and then sprayed on the coriander leaves at 30 L / mu. The amount of the basic selenium-rich liquid fertilizer in step (2) is changed. It is found that with the increase in the amount of fertilizer, the selenium content in the coriander first increases and then decreases, indicating that the application of the selenium-rich liquid fertilizer in this application has a certain dosage effect. In addition, it is found that only foliar spraying without root application does not significantly increase the selenium content.

[0147] Depend on Figure 2 It can be seen that 4 days after the coriander seedlings emerged, basic selenium-rich liquid fertilizer was used for root application at a fertilizer rate of 45 L / mu. The foliar spraying amount of the mixture of basic selenium-rich liquid fertilizer and growth regulator in step (3) was changed. It was found that with the increase in the spraying amount, the selenium content in coriander first increased and then decreased, and also showed a certain dosage effect. It can be seen that in the planting method of the present application, the root application in step (2) and the foliar fertilization in step (3) have a certain synergistic effect, and the two cooperate with each other to significantly increase the selenium content.

[0148] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A selenium-rich liquid fertilizer, characterized in that: The selenium-rich liquid fertilizer comprises basic selenium-rich liquid fertilizer and a growth regulator.

2. A selenium-rich liquid fertilizer according to claim 1, characterized in that: The preparation of the basic selenium-rich liquid fertilizer comprises the following steps: (1) drying corn cobs, rice straws, purple sweet potato vines, and kudzu vines to constant weight, respectively, and crushing them in a plant grinder in a mass ratio of corn cobs: rice straws: purple sweet potato vines: kudzu vines = 1:2-3:0.5-0.7:0.3-0.5 to obtain a mixed powder for later use; (2) immersing the obtained mixed powder in a treatment agent, subjecting the mixed powder to constant temperature ultrasonic stirring for 3 to 5 hours, and then placing the mixed powder in a high temperature and high pressure cooker for high temperature and high pressure treatment, followed by filtering, collecting the filtrate and concentrating it to 40 to 60% of the original volume to obtain a crop concentrate for later use; (3) After the crop concentrate and sterile water are mixed at a volume ratio of 1:8-12, manganese sulfate, magnesium sulfate and sodium chloride are added, stirred and mixed, and then sterilized. After cooling to room temperature, Bacillus subtilis is inoculated, and Na2SeO3 is added after culturing in a shaking incubator for 3-5 hours. The culture is continued for 2-4 days, and then filtered to obtain a basic selenium-rich liquid fertilizer.

3. A selenium-rich liquid fertilizer according to claim 2, characterized in that: The components and corresponding weight percentages of the treatment agent described in step (2) are: 0.7-1.3% fulvic acid, 1-2% lanthanum nitrate, 0.8-1.4% cerium nitrate, 7-10% phosphate amphoteric surfactant, and the balance is pure water.

4. A selenium-rich liquid fertilizer according to claim 2, characterized in that: The constant temperature described in step (2) is 50-70°C.

5. A selenium-rich liquid fertilizer according to claim 2, characterized in that: The high temperature and high pressure treatment in step (2) is carried out at a temperature of 100 to 120° C. and a pressure of 0.2 to 0.5 MPa.

6. A selenium-rich liquid fertilizer according to claim 2, characterized in that: The final concentrations of manganese sulfate, magnesium sulfate and sodium chloride described in step (3) are 0.02-0.03 mg / mL, 0.1-0.2 mg / mL and 0.1-0.3 mg / mL respectively.

7. A selenium-rich liquid fertilizer according to claim 2, characterized in that: The inoculation amount of Bacillus subtilis described in step (3) is 2-3%; The culture temperature is 35-39°C and the shaking speed is 120-150 rpm.

8. A selenium-rich liquid fertilizer according to claim 1, characterized in that: The preparation method of the growth regulator is as follows: adding brown algae oligosaccharide to deionized water under ultrasonic conditions, ultrasonically stirring at 60-70° C. for 30-50 minutes, adding glycidyl trimethylammonium chloride while stirring, fully stirring and mixing, heating to 80-90° C. for reaction for 20-30 minutes, cooling to 50-60° C., adding deionized water and amino acid chelate, stirring at a constant temperature for 30-40 minutes, cooling to room temperature, and then adding glutathione and silk fibroin, and continuing to stir for 1-2 hours.

9. A selenium-rich liquid fertilizer according to claim 8, characterized in that: In 1L of the growth regulator, the added amounts of the various raw materials are: 0.6-1g of brown algae oligosaccharide, 1-1.5g of glycidyl trimethylammonium chloride, 0.8-1.2g of amino acid chelate, 2-3g of glutathione, and 8-12g of silk fibroin.

10. A method for planting selenium-enriched coriander, characterized in that: The steps include: (1) Apply basal fertilizer to the soil before planting; (2) 4 to 5 days after the coriander seedlings emerge, apply the above-mentioned basic selenium-rich liquid fertilizer to the roots; (3) When the coriander seedlings grow to a height of 8 to 12 cm, mix the above-mentioned basic selenium-rich liquid fertilizer and growth regulator in a ratio of 70 to 90:1 and spray them on the coriander leaves.

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