Bactericidal composition containing melanin biosynthesis inhibitor and application thereof

Abstract

The invention relates to a bactericidal composition containing a melanin biosynthesis inhibitor. The invention also relates to an application of the bactericidal composition in the field of agriculture, forestry or gardening for preventing or preventing plant pathogenic bacteria from infecting plants. The invention provides a bactericidal composition containing a melanin biosynthesis inhibitor, the bactericidal composition comprises an active component A and an active component B, the active component A is a compound represented by a formula (I), the active component B is the melanin biosynthesis inhibitor, and the active component A is a compound represented by a formula (II). The melanin biosynthesis inhibitor is selected from any one of fenoxanil, tricyclazole, cypropamid and probenazole. The bactericidal composition disclosed by the invention shows an excellent control effect on various plant pathogenic bacteria, the control effect shows a synergistic effect under a certain mass ratio, the dosage can be effectively reduced, the agricultural input cost is reduced, and the drug resistance of the plant pathogenic bacteria is delayed.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide compounding, and specifically relates to a bactericidal composition containing a melanin biosynthesis inhibitor and application thereof. Background Art

[0002] Melanin biosynthesis inhibitor fungicides inhibit the formation of melanin in attached spores, making pathogens lose the ability to penetrate the host, thereby protecting plants from damage. Melanin biosynthesis inhibitor fungicides such as tricyclazole, pyraclostrobin, cyproconazole, and propibenthiazol are systemic and can be absorbed and conducted by plants. They have a protective effect but little therapeutic effect and cannot effectively inhibit spore germination, mycelial growth, and attached spore formation.

[0003] Rice blast is a kind of worldwide fungal disease caused by Pyricularia oryzae. All rice-growing areas have the occurrence of rice blast, which causes serious losses to rice production. Since rice blast can occur during the entire growth period of rice, it mainly infringes on rice seedlings, leaves, ears, nodes and other parts, causing heavy economic losses. The current varieties of drugs for preventing and treating rice blast are very limited at home and abroad. Research on the synergistic combination application technology of fungicides with different action modes is a major production demand for reducing the dosage of fungicides and the risk of resistance. The present invention uses a compound of formula (I) in combination with a melanin biosynthesis inhibitor fungicide such as tricyclazole, rice blast amide, cyclopropane amide, and allyl thiazole. It is surprisingly found that under a certain mass ratio, it has a synergistic effect on rice diseases, can significantly reduce the amount of chemical pesticides used, and reduce the risk of pathogens producing resistance to a single agent. Summary of the invention

[0004] Based on the above situation, the purpose of the present invention is to provide a fungicide composition containing a melanin biosynthesis inhibitor and its application. The fungicide composition is compounded by different active ingredients, and exhibits excellent prevention and control effects on a variety of plant pathogens. Under a certain mass ratio, the prevention effect manifests as a synergistic effect, which can effectively reduce the amount of medicine used, reduce agricultural input costs, and delay the drug resistance of plant pathogens.

[0005] In order to achieve the above object, the present invention adopts the following technical scheme: a bactericidal composition containing a melanin biosynthesis inhibitor, the bactericidal composition comprises an active ingredient A and an active ingredient B, the active ingredient A is a compound represented by formula (I), and the chemical structure is: The active ingredient B is a melanin biosynthesis inhibitor;

[0006] Furthermore, the melanin biosynthesis inhibitor is any one of pyraclostrobin, tricyclazole, cyproconazole, and propiconazole;

[0007] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:37 to 18:1 or any value within the above numerical range;

[0008] Furthermore, the mass ratio of the compound of formula (I) to pyrifosamide is 1:37 to 18:1;

[0009] Furthermore, the mass ratio of the compound of formula (I) to pyrifosamide is 1:18 to 16:1;

[0010] Furthermore, the mass ratio of the compound of formula (I) to pyrifosamide is 1:37, 1:18, 1:7, 1:4, 8:15, 4:3, 8:1, 16:1;

[0011] Furthermore, the mass ratio of the compound of formula (I) to tricyclazole is 1:20 to 18:1;

[0012] Furthermore, the mass ratio of the compound of formula (I) to tricyclazole is 1:20 to 15:1;

[0013] Further, the mass ratio of the compound of formula (I) to tricyclazole is 1:20, 1:9, 2:5, 1:1, 5:1, 15:1, 18:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to cyproconazole is 1:32 to 12:1;

[0015] Furthermore, the mass ratio of the compound of formula (I) to cyproconazole is 1:30 to 12:1;

[0016] Further, the mass ratio of the compound of formula (I) to cyproconazole is 1:32, 1:30, 1:20, 2:15, 3:2, 5:1, 12:1;

[0017] Furthermore, the mass ratio of the compound of formula (I) to allylbenzazole is 1:25 to 15:1;

[0018] Further, the mass ratio of the compound of formula (I) to allylbenzazole is 1:25, 1:10, 1:5, 5:1, 15:1;

[0019] Furthermore, the total weight of the bactericidal composition is 100 wt%, and the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 1 to 80 wt% or any value within the above numerical range;

[0020] Furthermore, the total weight of the bactericidal composition is 100 wt%, and the sum of the content of the active ingredient A and the active ingredient B in the bactericidal composition is 2 to 50 wt%;

[0021] Furthermore, the fungicidal composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredients;

[0022] Further, the auxiliary ingredients include one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a stabilizer, a film former, a warning color, a penetrant, and a carrier;

[0023] Further, the wetting agent is selected from a mixture of one or more of sodium dodecylbenzene sulfate, sodium dodecylbenzene sulfonate, saponin powder, alkyl sulfate, pull-open powder BX, silkworm feces, alkylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkanolamide polyoxyethylene ether and its phosphate or sulfate ester salt, and alkyl polyoxyethylene ether succinate sulfonate;

[0024] Further, the dispersant is selected from a mixture of one or more of polycarboxylates, lignin sulfonates, naphthalene or alkylnaphthalene formaldehyde condensate sulfonates, alkylbenzene calcium sulfonates, alkylphenol polyoxyethylene phosphates, fatty alcohol polyoxyethylene polyoxypropylene ethers, fatty alcohol polyoxypropylene polyoxypropylene ethers, and polymerized alkyl aryl sulfonates;

[0025] Further, the emulsifier is selected from a mixture of one or more of fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenylethylphenol polyoxyethylene polyoxypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrylphenol polyoxyethylene ether, and fatty acid polyoxyethylene ester;

[0026] Furthermore, the thickener is selected from a mixture of one or more of xanthan gum, polyvinyl alcohol, organic bentonite, magnesium aluminum silicate, and carboxymethyl cellulose;

[0027] Further, the disintegrant is selected from a mixture of one or more of aluminum chloride, bentonite, sucrose, modified starch, cellulose, urea, sodium carbonate, sodium bicarbonate, sodium chloride, sodium sulfate, citric acid and tartaric acid;

[0028] Furthermore, the antifreeze agent is selected from a mixture of one or more of alcohols, alcohol ethers, and inorganic salts;

[0029] Furthermore, the defoaming agent is selected from silicone oil, C 10 ~C 20 Saturated fatty acid compounds, C8~C 10 A mixture of one or more fatty alcohol compounds or silicone compounds;

[0030] Further, the solvent is selected from a mixture of one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, diesel, N,N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives and deionized water;

[0031] Further, the preservative is selected from a mixture of one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, and methyl p-hydroxybenzoate;

[0032] Further, the stabilizer is selected from a mixture of one or more of oxalic acid, succinic acid, adipic acid, borax, and epoxidized vegetable oil;

[0033] Furthermore, the warning color is selected from any one or more adjustment colors of blue, green, red, purple, and yellow;

[0034] Further, the film-forming agent is selected from one or more of sodium carboxymethyl starch, sodium carboxymethyl cellulose, sodium alginate, polyvinyl alcohol, and polyacrylic acid;

[0035] Furthermore, the carrier is selected from a mixture of one or more of kaolin, bentonite, attapulgite, light calcium carbonate, diatomaceous earth, and white carbon black;

[0036] Furthermore, the fungicide composition can be prepared into any formulation allowed in agriculture, and the formulation is a solid formulation, a liquid formulation or a seed treatment formulation;

[0037] Furthermore, the solid preparation is a wettable powder or a water-dispersible granule, the liquid preparation is a suspension, an emulsifiable concentrate, an aqueous emulsion, or a microemulsion, and the seed treatment preparation is a seed treatment suspension.

[0038] A method for controlling or preventing plant pathogens from infecting plants, wherein a fungicide composition containing a melanin biosynthesis inhibitor is used to control agricultural, forestry or horticultural plant pathogens;

[0039] Furthermore, the agricultural, forestry or horticultural plants are rice, wheat, corn, sorghum, tobacco and cotton;

[0040] Furthermore, the agricultural, forestry or horticultural plant is rice;

[0041] Furthermore, the fungicidal composition is applied in an effective amount to the plant pathogenic bacteria and / or its environment, or to the plant, plant propagation material and subsequently grown plant organs, soil or cultivation medium, material or space.

[0042] The present invention has the following advantages over the prior art:

[0043] 1) The fungicidal composition of the present invention not only expands the control spectrum, but also prolongs the use period of the agent and widens the suitable control period;

[0044] 2) The bactericidal composition of the present invention can delay the development of resistance in pathogens;

[0045] 3) The bactericidal composition of the present invention has excellent control effect under a certain mass ratio, and the synergistic effect is obvious. DETAILED DESCRIPTION

[0046] In order to better illustrate the effective control effect of the present invention, the above-mentioned embodiment agent is used to make the technical scheme, purpose and advantages of the present invention clearer. The present invention is described with the following specific embodiments, but the present invention is not limited to these examples.

[0047] Preparation Example:

[0048] Preparation Example 1: 40% compound of formula (I)·pasteamide water dispersible granules (20:20)

[0049] Formula composition: 20% compound of formula (I), 20% rice blastamide, 3.5% sodium dodecyl sulfate, 4% polynaphthaldehyde sulfonate, 1% fatty alcohol polyoxyethylene ether sodium sulfate, 6% polycarboxylic acid sodium salt, 4.5% sodium lignin sulfonate, 5% attapulgite, and kaolin makes up the balance;

[0050] Preparation method: according to the formula ratio, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% of water is added, and then kneading, granulation, drying and sieving are carried out to obtain a water-dispersible granule product; or the ground powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain a water-dispersible granule product.

[0051] Preparation Example 2: 60% compound of formula (I)·tricyclazole water dispersible granules (30:30)

[0052] Formula composition: 30% compound of formula (I), 30% tricyclazole, 8% polynaphthaldehyde sulfonate, 1% sodium sulfate of fatty alcohol polyoxyethylene ether, 2.5% BX powder, 5% sodium lignin sulfonate, 5% attapulgite, and kaolin makes up the balance;

[0053] Preparation method: Same as Preparation Example 1.

[0054] Preparation Example 3: 58% compound of formula (I)·cycloheximide water dispersible granules (32:26)

[0055] Formula composition: 32% compound of formula (I), 26% cyproconazole, 8% polynaphthaldehyde sulfonate, 1% sodium sulfate of fatty alcohol polyoxyethylene ether, 2% BX powder, 4.5% sodium lignin sulfonate, 10% bentonite, and kaolin makes up the balance;

[0056] Preparation method: Same as Preparation Example 1.

[0057] Preparation Example 4: 50% compound of formula (I)·allylbenzazole water dispersible granules (10:40)

[0058] Formula composition: 10% compound of formula (I), 40% allylbenzazole, 5.5% sodium salt of polycarboxylic acid, 3.5% polynaphthaldehyde sulfonate, 1% sodium sulfate of fatty alcohol polyoxyethylene ether, 2% BX powder, 4.5% sodium lignin sulfonate, 10% bentonite, and kaolin makes up the balance;

[0059] Preparation method: Same as Preparation Example 1.

[0060] Preparation Example 5: 10% compound of formula (I)·pasteamide emulsifiable concentrate (6:4)

[0061] Formula composition: 6% compound of formula (I), 4% rice blastamide, 15% propylene carbonate, 15% DMF, 15% cyclohexanone, 4% calcium dodecylbenzenesulfonate, 12% fatty alcohol polyoxyethylene ether, and methyl oleate makes up the balance;

[0062] Preparation method: Add the active ingredients into the co-solvent according to the formula ratio of the embodiment, and add the surfactant and other functional additives thereto, stir and mix them evenly in a stirring mixing kettle to obtain the emulsifiable concentrate.

[0063] Preparation Example 6: 28% formula (I) compound·pasteamide suspension (16:12)

[0064] Formula composition: 16% compound of formula (I), 12% rice blastamide, 1.5% Guerbet alcohol polyoxyethylene ether, 1% alkyl polyoxyethylene ether sodium sulfonate, 3% styrene phenol polyoxyethylene ether phosphate, 2% phenethylphenol polyoxyethylene polyoxypropylene ether, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% glycerol, 0.1% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0065] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added to mix evenly, and the suspension is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0066] Preparation Example 7: 30% formula (I) compound·cycloheximide suspension (18:12)

[0067] Formula composition: 18% compound of formula (I), 12% cyproconazole, 2% phenethylphenol polyoxyethylene polyoxypropylene ether, 1.8% Guerbet alcohol polyoxyethylene ether, 1.2% alkyl polyoxyethylene ether sodium sulfonate, 3% styrene phenol polyoxyethylene ether phosphate, 0.25% xanthan gum, 0.8% magnesium aluminum silicate, 5% glycerol, 0.1% potassium benzoate, 0.5% organosilicon defoamer, and deionized water to make up the balance;

[0068] Preparation method: Same as Preparation Example 6.

[0069] Preparation Example 8: 80% compound of formula (I)·tricyclazole wettable powder (35:45)

[0070] Formula composition: 35% compound of formula (I), 45% tricyclazole, 5% sodium lignin sulfonate, 4% alkyl naphthalene sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 10% kaolin, 8% starch, and bentonite makes up the balance;

[0071] Preparation method:

[0072] Preparation Example 9: 45% compound of formula (I)·allylbenzazole wettable powder (20:25)

[0073] Formula composition: 20% compound of formula (I), 25% allylbenzazole, 4.5% naphthalene sulfonate, 1.5% sodium lignin sulfonate, 2% sodium dodecyl sulfate, 3% sodium polycarboxylate, 5% white carbon black, kaolin makes up the balance

[0074] Preparation method: Same as Preparation Example 8.

[0075] Indoor activity test

[0076] Embodiment 1:

[0077] Indoor joint action test on rice blast

[0078] Test basis: NY / T 1156.8-2007 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 8: Pot Method for Control of Rice Blast", NY / T 1156.6-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 6: Determination of Combined Action of Mixtures".

[0079] Experimental target: Rice blast fungus (Pyricularia oryae), wild-type strain of rice blast fungus collected, isolated and preserved in the laboratory.

[0080] Test crops: Rice. After germination, 20 seeds were sown in each pot, and 10 rice seedlings with 3 leaves and 1 heart with consistent growth were selected for use.

[0081] Test agents: technical drug of the compound of formula (I), tricyclazole technical drug, rice blastamide technical drug, cyproconazole technical drug, and propionamide technical drug.

[0082] Preparation of spore suspension: The pathogen was cultured on a culture medium (SDC medium, etc.), and after spores were produced, the spores were washed with sterile water and filtered through three layers of gauze to prepare a suspension with a concentration of 1×10 5 Prepare a suspension of spores / mL for later use.

[0083] Drug preparation: Choose a suitable solvent (methanol or dimethyl sulfoxide, etc.) to dissolve, and dilute with 0.1% Tween 80 aqueous solution. According to the activity of the drug, set 5 series of mass concentrations, and the final content of organic solvent generally does not exceed 0.5%.

[0084] Chemical treatment: Spray the solution evenly on the leaves until they are all wet. Wait for the solution to dry naturally and set aside. Treat 1 pot each time, repeat 4 times, and set a treatment containing only solvents and surfactants but no active ingredients as a blank control.

[0085] Inoculation and cultivation: Spray inoculation of spore suspension.

[0086] 1) Protective: inoculation 24 hours after drug treatment;

[0087] 2) Therapeutic: inoculated 24 hours before drug treatment;

[0088] After inoculation, move to an incubator (relative humidity above 95%, keep condensation on the leaves, temperature 25℃~26℃) and culture in the dark for 24h; then culture at 25℃~26℃, 12h light and dark alternation, relative humidity 86% for 7d.

[0089] Investigation: When the rate of blank control leaves reaches more than 50%, conduct graded investigation on the disease status of the leaves of the whole plant.

[0090] The grading method is:

[0091] Level 0: The whole plant is disease-free;

[0092] Level 1: Brown spots appear;

[0093] Level 3: Typical spindle-shaped lesions appear, and the lesion area accounts for less than 5% of the entire leaf area;

[0094] Level 5: Typical lesions, with the lesion area accounting for 6% to 25% of the entire leaf area;

[0095] Level 7: Typical lesions, with the lesion area accounting for 26% to 50% of the entire leaf area;

[0096] Level 9: Typical lesions, with the lesion area accounting for more than 50% of the total leaf area.

[0097] The efficacy is calculated according to the following formula:

[0098]

[0099] Statistical analysis: Based on the survey results, the data were subjected to regression analysis using DPS (data processing system) to calculate the EC of each drug. 50 and its 95% confidence limits, and a significant difference analysis was performed among the treatments.

[0100] The co-toxicity coefficient (CTC) of the mixture was calculated according to Sun Yunpei's method to evaluate the type of combined action.

[0101] CTC≥120 showed a synergistic effect, CTC≤80 showed an antagonistic effect, and CTC between 80 and 120 showed an additive effect.

[0102] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0103]

[0104] Where:

[0105] ATI——Actual Toxicity Index of Mixture;

[0106] S——EC of standard fungicide 50 , the unit is milligrams per liter (mg / L);

[0107] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0108] TTI=TI A *P A +TI B *P B

[0109] Where:

[0110] TTI – Theoretical Toxicity Index of Mixtures;

[0111] TI A ——A: toxicity index of the agent;

[0112] P A ——The percentage of agent A in the mixture, in percentage (%);

[0113] TI B ——Toxicity index of agent B;

[0114] P B ——The percentage of agent B in the mixture, in percentage (%).

[0115]

[0116] Where:

[0117] CTC – Co-toxicity coefficient;

[0118] ATI——Actual Toxicity Index of Mixture;

[0119] TTI - Theoretical Toxicity Index of Mixture.

[0120] Indoor test results:

[0121] The results in Table 1 show that the compounds of formula (I) all showed very good protective and therapeutic effects on rice blast. Blastamide, tricyclazole, cyproconazole and allylbenzazole showed good protective effects, but the therapeutic effects of tricyclazole, cyproconazole and allylbenzazole were relatively poor.

[0122] Table 1 EC values ​​of different agents for the prevention or treatment of rice blast 50 (mg.L -1 )

[0123]

[0124] The test results show that the compound of formula (I) and rice blastamide mixed in a ratio of 1:18-16:1 showed synergism; the compound of formula (I) and tricyclazole mixed in a ratio of 1:20-15:1 showed synergism; the compound of formula (I) and cyclopropane amide mixed in a ratio of 1:30-12:1 showed synergism; the compound of formula (I) and allylbenzazole mixed in a ratio of 1:25-15:1 showed synergism.

[0125] Table 2 Results of indoor combined action test of compound of formula (I) and blastamide against rice blast

[0126]

[0127] Table 3 Results of indoor combined action test of compound of formula (I) and tricyclazole against rice blast

[0128]

[0129] Table 4 Results of indoor combined action test of compound of formula (I) and cyproconazole against rice blast

[0130]

[0131]

[0132] Table 5 Results of indoor combined action test of compound of formula (I) and allylbenzazole on rice blast

[0133]

[0134] Example 2: Field efficacy test on rice blast

[0135] Test basis: The test refers to GB / T 17980.19-2000 "Guidelines for field efficacy tests (I) Fungicides for the control of rice foliar diseases".

[0136] Test location: The test was conducted in the rice fields of Fuxing Village, Xifeng County, Tieling City, Liaoning Province. The test site has flat terrain, uniform fertility, and white pulp soil. The crop variety, planting period, growth, fertilizer and water management conditions of each test plot are basically the same.

[0137] Test target: Rice blast fungus.

[0138] Experimental crops: Rice (Liaoxing No. 10)

[0139] Experimental design: The experimental design has 8 treatments, each treatment is repeated 4 times, and clean water is used as a blank control. The area of ​​the plot is 60m 2 , each plot is randomly arranged in blocks, and protection rows are set up in adjacent plots.

[0140] Test drug:

[0141] Table 6 Test agents and dosage

[0142]

[0143]

[0144] Application time and number of times: The test was conducted at the early stage of rice cracking (crack rate 5% to 10%), with a water consumption of 30 kg per mu, and a 3WBD-16L electric sprayer was used for uniform spraying. Before and during the test, each area used the same pesticide (without any impact on the test pesticide) for other pests and diseases control, and the management level of each area was consistent.

[0145] Survey method: Five sampling points are adopted in each plot, with 50 ears at each point, and a total of 250 ears are surveyed in each plot, for one survey in total. Before the rice matures and is harvested, the number of diseased ears at all levels in each plot is surveyed, and the disease index and control effect are calculated.

[0146] Safety investigation of pesticides: During the test, rice grew normally, and the pesticides in the tested concentrations did not cause any damage to rice plants or other non-target organisms.

[0147] The classification records are made according to the following classification method:

[0148] Level 0: no disease;

[0149] Level 1: less than 5% loss per ear (individual branches and stalks are diseased);

[0150] Level 3: 6% to 20% loss per ear (about one-third of the branches and stalks are diseased);

[0151] Level 5: 21% to 50% loss per ear (the neck or main axis of the ear is diseased, and the grains are half-shrunken);

[0152] Level 7: 51% to 70% loss per ear (the neck of the ear is diseased and most of the grains are shrunken);

[0153] Level 9: 71% to 100% loss per ear (the ear neck becomes diseased, resulting in white ears).

[0154] The efficacy is calculated according to the following formula:

[0155]

[0156]

[0157] The results of the field efficacy test are shown in the following table:

[0158] Table 7 Field efficacy test results of different pesticides against rice blast

[0159]

[0160]

[0161] From the analysis of the field efficacy test results in the above table, it can be seen that the compound of formula (I) and blastamide, tricyclazole, cyclopropane, and allylbenzazole have a good control effect on rice blast. After the rice matures and is harvested after the application, the rice panicle blast is investigated. The control effects of 28% formula (I) compound·blastamide suspension (16:12), 80% formula (I) compound·tricyclazole wettable powder (35:45), 30% formula (I) compound·cyclopropane suspension (18:12), and 50% formula (I) compound·allylbenzazole water dispersible granules (10:40) are all greater than 90%, namely 90.79%, 93.69%, 90.50%, and 90.65%, respectively, which are significantly higher than the control single agent.

[0162] In summary, through indoor toxicity determination and field efficacy test, it can be seen that the fungicidal composition of the compound of formula (I) of the present invention and pyraclostrobin, tricyclazole, cyproconazole and propionylthiazole has a good control effect on plant pathogens, is safe for target crops, has a significant control effect, is superior to a single agent in delaying the development of drug resistance and prolonging the lasting effect, can effectively reduce costs and reduce drug residues.

[0163] Although the application describes specific embodiments in detail by means of examples, the disclosure of the application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the application is not limited to the disclosed specific forms. On the contrary, the disclosure of the application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the application, and the scope of the application is limited by the attached claims and their legal equivalents.

Claims

1. A bactericidal composition containing a melanin biosynthesis inhibitor, characterized in that: The bactericidal composition comprises active ingredient A and active ingredient B, wherein the active ingredient A is a compound represented by formula (I), and the chemical structure is: The active ingredient B is a melanin biosynthesis inhibitor.

2. The bactericidal composition according to claim 1, characterized in that The melanin biosynthesis inhibitor is any one of pyraclostrobin, tricyclazole, cyproconazole and propiconazole.

3. The bactericidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:50 to 30:1; Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:37 to 18:1; The active ingredient B is pyrifosamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:37 to 16:1; The active ingredient B is tricyclazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 18:1; The active ingredient B is cyproconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 12:1; The active ingredient B is allylbenzazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 15:1; Preferably, the active ingredient B is pyrifosamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:18 to 16:1; The active ingredient B is tricyclazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 15:1; The active ingredient B is cyproconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 12:1; The active ingredient B is allylbenzazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 15:

1.

4. The bactericidal composition according to claim 1, characterized in that The total weight of the bactericidal composition is 100 wt %, and the sum of the content of the active ingredient A and the active ingredient B in the bactericidal composition is 1 to 80 wt %; Preferably, the total weight of the bactericidal composition is 100 wt%, and the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 2 to 50 wt%.

5. The bactericidal composition according to claim 1, characterized in that The bactericidal composition comprises auxiliary ingredients in addition to the active ingredients.

6. The bactericidal composition according to claim 5, characterized in that The auxiliary components include one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a stabilizer, a film former, a warning color, a penetrant and a carrier.

7. The bactericidal composition according to claim 1, characterized in that The bactericidal composition can be prepared into any formulation form permitted in agriculture, and the formulation form is a solid formulation, a liquid formulation or a seed treatment formulation.

8. A method for controlling or preventing plant pathogens from infecting plants, characterized in that: The fungicidal composition according to claim 1 is used for preventing and controlling pathogenic bacteria in agricultural, forestry or horticultural plants.

9. The method according to claim 8, characterized in that The agricultural, forestry or horticultural plants are rice, wheat, corn, sorghum, tobacco and cotton; Preferably, the agricultural, forestry or horticultural plant is rice.

10. The method according to claim 8, characterized in that The fungicidal composition is applied in an effective amount to the plant pathogenic bacteria and / or its environment, or to plants, plant propagation materials and subsequently grown plant organs, soil or cultivation media, materials or spaces.

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