Method for inducing cultivation of calculus bovis in ox gall through solvent exchange phase change gel

Abstract

The invention discloses a method for cultivating calculus bovis in ox gall induced by solvent exchange phase change gel. The problem that calculus bovis is not suitable for cultivation in the prior art is solved. The method comprises the following steps: S1, selecting calves, wherein the calves are 4-8-month-old calves; s2, mixing 45%-55% of egg yolk lecithin, 12%-18% of medium-chain and long-chain fatty acid, 18%-22% of ethyl cellulose and the balance of ethyl alcohol to prepare an injectable solution; s3, injecting 6 to 12 ml of the injectable solution into the ox gall; s4, injecting calculus bovis conversion flora to infect the cattle; and S5, after the cattle are normally fed for 1-1.5 years, killing the cattle and taking yellow. After the injectable solution is injected into the ox gall, water in bile exchanges with ethanol in the preparation, the injectable solution is gradually and completely converted into a gel state from a solution state after 8 hours and exists in the ox gall, and the injectable solution is gradually absorbed and metabolized along with gradual absorption and metabolism of egg yolk lecithin; bezoar crystals are gradually separated out on the surface with ethyl cellulose as a framework.

Description

Technical Field

[0001] The invention belongs to the technical field of bezoar cultivation, and particularly relates to a method for cultivating bezoar in cattle gall bladder induced by solvent exchange phase change gel. Background Art

[0002] Calculus bovis is a gallstone with bilirubin as the main component, containing bile acid, deoxycholic acid, cholesterol, ergot cholesterol, fatty acids, lecithin, mucin, calcium and metal ions, etc. In addition, it also contains glutalanine, glycine, taurine, aspartic acid, arginine, leucine, threonine, serine, glutamic acid, cystine, valine, leucine, isoleucine, tyrosine, phenylalanine, lysine, histidine, proline and methionine, etc. Calculus bovis is usually divided into four categories: natural bezoar naturally generated in cattle, cultured bezoar artificially cultivated in cattle, artificially synthesized artificial bezoar and in vitro cultured bezoar cultivated in vitro, among which natural bezoar has low yield and high price; in vivo cultivation of bezoar is technically difficult and has limited yield; the effect of artificially synthesized bezoar is not as ideal as other bezoars.

[0003] The main method of cultivating bezoar in vivo is to place a foreign nucleus, such as pumice wrapped in Dacron cloth or an egg-sized plastic product, in the gallbladder of the cattle, and inject a bacterial solution for producing bezoar into the gallbladder. Under the action of the metabolic products of the bacterial cells, the bilirubin metal salt in the bile precipitates and gradually deposits on the foreign nucleus, thereby forming a bezoar with a certain volume. At present, cultivating bezoar in vivo requires surgical implantation of a bezoar bed (such as a plastic product), which has high surgical costs, long recovery time after surgery, and is prone to infection during surgery and postoperative recovery. Summary of the invention

[0004] The present invention provides a method for cultivating bezoar in cattle gall by inducing solvent exchange phase change gel, so as to at least solve part of the above technical problems.

[0005] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0006] A method for cultivating bezoar in cattle gall bladder induced by solvent exchange phase change gel comprises the following steps:

[0007] S1. Select calves, which are 4-8 months old cattle;

[0008] S2, mixing 45%-55% of egg yolk lecithin, 12%-18% of medium-chain fatty acids, 18%-22% of ethyl cellulose, and the remainder of ethanol to prepare an injectable solution;

[0009] S3, inject 6-12 ml of the injectable solution into the cow gall;

[0010] S4, injecting bezoar to convert the bacterial flora and infect the cattle;

[0011] S5. After 1-1.5 years of normal feeding, the cattle are killed and the yellow meat is taken.

[0012] Further, the following steps are included:

[0013] S1. Select calves, which are 6-month-old cattle;

[0014] S2, mixing 50% egg yolk lecithin, 15% medium-chain fatty acids, 20% ethyl cellulose, and the remainder ethanol to prepare an injectable solution;

[0015] S3, inject 10 ml of the injectable solution into the cow's gallbladder,

[0016] S4, injecting bezoar to convert the bacterial flora and infect the cattle;

[0017] S5. After 1.5 years of normal feeding, the cattle are killed and the yellow meat is taken.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The invention is scientifically and rationally designed and easy to operate. The invention adopts an injection solution into the cow gallbladder, and the water in the bile is exchanged with the ethanol in the preparation. The injectable solution is gradually and completely transformed from a solution state to a gel state and exists in the cow gallbladder after 8 hours. After 21 days, the egg yolk lecithin is gradually absorbed and metabolized. Since the ethyl cellulose is not absorbed, the bezoar crystals are gradually precipitated on the surface with the ethyl cellulose as the skeleton, which can facilitate the cultivation of the bezoar. The solution is injected in a minimally invasive manner, which can effectively reduce the infection risk of the cow. One injection can be successful, the success rate is high, and the yellow production rate is high. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical scheme and advantages of the present invention more clear, the present invention is further described in detail below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Example 1.

[0022] The present invention provides a method for inducing the cultivation of bezoar in cattle gallbladder by using solvent exchange phase change gel, comprising the following steps:

[0023] S1. Select calves, which are 6-month-old cattle;

[0024] S2, mixing 45% egg yolk lecithin, 12% medium-chain fatty acids, 22% ethyl cellulose, and the remainder ethanol to prepare an injectable solution;

[0025] S3, injecting 10 ml of the injectable solution into the cow gallbladder;

[0026] S4, injecting bezoar to convert the bacterial flora and infect the cattle;

[0027] S5. After one year of normal feeding, the cattle are killed and the yellow meat is taken.

[0028] When the injectable solution of the present invention is injected into the bile of cattle, water in the bile is exchanged with ethanol in the preparation, and the injectable solution gradually and completely changes from a solution state to a gel state and exists in the bile of cattle after 8 hours. After 21 days, the egg yolk lecithin is gradually absorbed and metabolized, and since ethyl cellulose is not absorbed, bezoar crystals are gradually precipitated on the surface with ethyl cellulose as the skeleton.

[0029] Bezoar testing method: After being fed with cultured bezoar cattle for one year, the cultured bezoar in the gallbladder is obtained through surgical laparotomy. The obtained bezoar is dried and weighed, and the cultured bezoar production of each cow is recorded; then the content of bilirubin and other main components of the cultured bezoar is determined according to the "Chinese Pharmacopoeia" (2020 edition).

[0030] The results showed that the bezoar production of the experimental cattle was 20.69 grams, the bilirubin content was higher than 35%, and the bile acid content was above 14%, which met the standards of high-quality bezoar.

[0031] Example 2.

[0032] The present invention provides a method for inducing the cultivation of bezoar in cattle gallbladder by using solvent exchange phase change gel, comprising the following steps:

[0033] S1. Select calves, which are 4-month-old cattle;

[0034] S2, mixing 50% egg yolk lecithin, 15% medium-chain fatty acids, 20% ethyl cellulose, and the remainder ethanol to prepare an injectable solution;

[0035] S3, inject 10 ml of the injectable solution into the cow gall;

[0036] S4, injecting bezoar to convert the bacterial flora and infect the cattle;

[0037] S5. After one year of normal feeding, the cattle are killed and the yellow meat is taken.

[0038] The bezoar detection method is the same as that in Example 1. The results show that the yield of the experimental cow is 19.35 grams, the bilirubin is higher than 35%, and the bile acid is above 14%, which meets the standards of high-quality bezoar.

[0039] Example 3.

[0040] The present invention provides a method for inducing the cultivation of bezoar in cattle gallbladder by using solvent exchange phase change gel, comprising the following steps:

[0041] S1. Select calves, which are 8-month-old cattle;

[0042] S2, mixing 55% egg yolk lecithin, 12% medium-chain fatty acids, 18% ethyl cellulose, and the remainder ethanol to prepare an injectable solution;

[0043] S3, inject 10 ml of the injectable solution into the cow gall,

[0044] S4, injecting bezoar to convert the bacterial flora and infect the cattle;

[0045] S5. After one year of normal feeding, the cattle are killed and the yellow meat is taken.

[0046] The bezoar detection method is the same as that in Example 1. The results show that the yield of the experimental cow is 17.98 grams, the bilirubin is higher than 35%, and the bile acid is above 14%, which meets the standards of high-quality bezoar.

[0047] Comparative example 1.

[0048] The present invention provides a method for inducing the cultivation of bezoar in cattle gallbladder by using solvent exchange phase change gel, comprising the following steps:

[0049] S1. Select calves, which are 6-month-old cattle;

[0050] S2, mixing 40% egg yolk lecithin, 10% medium-chain fatty acids, 12% ethyl cellulose, and the remainder ethanol to prepare an injectable solution;

[0051] S3, injecting 10 ml of the injectable solution into the cow gallbladder;

[0052] S4, injecting bezoar to convert the bacterial flora and infect the cattle;

[0053] S5. After 1.2 years of normal feeding, the cattle are killed and the yellow meat is taken.

[0054] The bezoar detection method is the same as that in Example 1. The results show that the output value of the bezoar of the test cattle is 14.45 grams, but the quality of the bezoar is poor, with bilirubin less than 35% and bile acid less than 14%.

[0055] Compare column 2.

[0056] The present invention provides a method for inducing the cultivation of bezoar in cattle gallbladder by using solvent exchange phase change gel, comprising the following steps:

[0057] S1. Select calves, which are 6-month-old cattle;

[0058] S2. Mix 60% egg yolk lecithin, 9% medium-chain fatty acids, 11% ethyl cellulose, and the remainder ethanol to prepare an injectable solution;

[0059] S3, injecting 10 ml of the injectable solution into the cow gallbladder;

[0060] S4, injecting bezoar to convert the bacterial flora and infect the cattle;

[0061] S5. After one year of normal feeding, the cattle are killed and the yellow meat is taken.

[0062] The bezoar detection method is the same as that in Example 1. The detection results show that the bezoar yield of the experimental cattle is 10.53 grams, the bilirubin is less than 35%, and the bile acid is less than 14%.

[0063] The yellowing results of Example 1, Example 2, Example 3, Comparative Example 1 and Comparative Example 2 are shown in Table 1.

[0064] Table 1 Bezoar production table

[0065]

[0066] The invention is scientifically and rationally designed and easy to operate. The invention adopts an injection solution into the cow gallbladder, and the water in the bile is exchanged with the ethanol in the preparation. The injectable solution is gradually and completely transformed from a solution state to a gel state and exists in the cow gallbladder after 8 hours. After 21 days, the egg yolk lecithin is gradually absorbed and metabolized. Since the ethyl cellulose is not absorbed, the bezoar crystals are gradually precipitated on the surface with the ethyl cellulose as the skeleton, which can facilitate the cultivation of the bezoar. The solution is injected in a minimally invasive manner, which can effectively reduce the infection risk of the cow. One injection can be successful, the success rate is high, and the yellow production rate is high.

[0067] Finally, it should be noted that the above embodiments are only preferred embodiments of the present invention to illustrate the technical solutions of the present invention, rather than limiting them, and certainly not limiting the patent scope of the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention. In other words, any changes or modifications made to the main design concept and spirit of the present invention that have no substantive significance, and the technical problems they solve are still consistent with the present invention, should be included in the protection scope of the present invention. In addition, the direct or indirect application of the technical solutions of the present invention in other related technical fields is also included in the patent protection scope of the present invention.

Claims

1. A method for inducing the cultivation of bezoar in cattle gallbladder by solvent exchange phase change gel, characterized in that: The following steps are involved: S1. Select calves, which are 4-8 months old cattle; S2, mixing 45%-55% of egg yolk lecithin, 12%-18% of medium-chain fatty acids, 18%-22% of ethyl cellulose, and the remainder of ethanol to prepare an injectable solution; S3, inject 6-12 ml of the injectable solution into the cow gall; S4, injecting bezoar to convert the bacterial flora and infect the cattle; S5. After 1-1.5 years of normal feeding, the cattle are killed and the yellow meat is taken.

2. The method for cultivating bezoar in cattle gall bladder induced by solvent exchange phase change gel according to claim 1, characterized in that: The following steps are involved: S1. Select calves, which are 6-month-old cattle; S2, mixing 50% egg yolk lecithin, 15% medium-chain fatty acids, 20% ethyl cellulose, and the remainder ethanol to prepare an injectable solution; S3, inject 10 ml of the injectable solution into the cow's gallbladder, S4, injecting bezoar to convert the bacterial flora and infect the cattle; S5. After 1.5 years of normal feeding, the cattle are killed and the yellow meat is taken.

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