Preparation method of ganoderma lucidum broken wall superfine powder for female insomnia

Abstract

The present application relates to the technical field of biological medicine, and discloses a preparation method of ganoderma lucidum broken wall superfine powder for female insomnia, after the ganoderma lucidum thick piece is treated by the processes of spraying moistening, hot pressure steam heating, ultrasonic treatment and microwave drying, the content of polysaccharide, triterpene and sterol is improved, and the release rate of active ingredients is also significantly improved. The structure of the ganoderma lucidum mycelium cell wall is damaged by the present application technology, so that the active ingredients such as polysaccharide, triterpene and sterol originally existing in the ganoderma lucidum can be easily extracted and released. The ganoderma lucidum cells are loosened and broken in the present application, and the polysaccharide in the combined state can be changed into the free state, so that the content of the ganoderma lucidum polysaccharide can be greatly improved. The active ingredients in the ganoderma lucidum can be quickly and maximally released in the digestive tract by directly taking the broken wall superfine powder of the broken cells, so that the effect of the superfine powder on improving female insomnia is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological medicine, in particular to a preparation method of ganoderma lucidum broken wall superfine powder for female insomnia. BACKGROUND

[0002] Ganoderma lucidum is a kind of dried fruiting body of Ganoderma applanatum or Ganoderma lucidum. It has the functions of tonifying qi and tranquilizing the mind, relieving cough and asthma. It can be used for restlessness of the heart, insomnia and palpitation, lung deficiency cough and asthma, and lack of appetite. Modern research shows that ganoderma lucidum has the effects of enhancing immunity, anti-tumor, anti-virus, anti-oxidation, anti-aging, anti-fatigue and anti-hypoxia. The active ingredients in ganoderma lucidum include polysaccharides, triterpenes and sterols, amino acids and polypeptides, nucleotides and alkaloids. Among them, the main active ingredient of ganoderma lucidum is polysaccharide. Therefore, the content of polysaccharide in ganoderma lucidum and the ease of release of effective components from ganoderma lucidum directly affect the effectiveness of ganoderma lucidum. Increasing the content of polysaccharide in ganoderma lucidum and increasing the release rate and amount of effective components in ganoderma lucidum are important means to improve the effectiveness of ganoderma lucidum.

[0003] Ganoderma lucidum polysaccharide exists in two forms: free and combined. Free polysaccharide, amino acid and small molecule polypeptide are soluble and can be utilized by the human body to play a role. Combined polysaccharide, protein and chitosan are insoluble, cannot be extracted and released by conventional methods, and cannot be utilized by the human body. According to the present application, the combined polysaccharide can be converted into free polysaccharide, thereby increasing the content of polysaccharide in ganoderma lucidum.

[0004] The mycelial wall of ganoderma lucidum fruiting body is thick and large, and is composed of glucan, chitin, cellulose, hemicellulose, lignin, protein and other cell wall materials. The texture of the cell wall is tough and has high mechanical strength, and is resistant to pressure and temperature. It is also very stable to digestive enzymes. The polysaccharide and other functional substances contained therein are difficult to be fully extracted and utilized. SUMMARY

[0005] The technical problem solved by the present application is to provide a preparation method of ganoderma lucidum broken wall superfine powder with high content of polysaccharide, triterpenes and sterols, which can be used to improve female insomnia.

[0006] The technical solution provided by the present application is a preparation method of ganoderma lucidum broken wall superfine powder for female insomnia.

[0007] (1) Dry Ganoderma applanatum or Ganoderma lucidum fruiting body, wash with water and cut into thick slices.

[0008] (2) Spray distilled water on the thick slices of ganoderma lucidum to make them wet. The spraying method is used instead of soaking, so that the thick slices of ganoderma lucidum are wetted without causing loss of active ingredients.

[0009] (3) Put the permeated Ganoderma lucidum thick slice into a hot-pressing steam cabinet, seal, vacuumize, and then introduce hot-pressing steam to heat-treat, and then quickly release the steam to cool.

[0010] (4) Put the heat-treated Ganoderma lucidum thick slice into an ultrasonic processor to perform ultrasonic treatment.

[0011] (5) Put the ultrasonic-treated Ganoderma lucidum thick slice into a microwave drying machine to dry.

[0012] (6) Crush the dried Ganoderma lucidum thick slice into ultrafine powder to obtain Ganoderma lucidum broken-wall ultrafine powder for female insomnia.

[0013] Preferably, in (1), the thickness of the Ganoderma lucidum thick slice is 2-4 mm.

[0014] Preferably, in (2), the permeation time is 2-4 h.

[0015] Preferably, in (3), the pressure in the hot-pressing steam cabinet is controlled to be 105-137 kPa by introducing hot-pressing steam, and the heating treatment time is 2-4 h.

[0016] Preferably, in (3), the hot steam in the hot-pressing steam cabinet is completely released within 2-5 min after heating treatment.

[0017] Preferably, in (4), the ultrasonic treatment conditions are as follows: power 200-300 W, frequency 20-25 kHz, and time 60-90 min.

[0018] Preferably, in (5), the microwave frequency in the microwave drying machine is 2400-2500 MHz, the drying temperature is 60-70℃, and the drying time is 20-30 min.

[0019] Preferably, in (6), the dried Ganoderma lucidum thick slice is broken-wall crushed into broken-wall ultrafine powder in an ultrafine crusher or an airflow crusher.

[0020] Preferably, in step (6), the quality requirements for the Ganoderma lucidum broken-wall ultrafine powder include the following contents: moisture ≦ 17%, water-soluble extract ≧ 3.0%, polysaccharide content ≧ 2.00%, triterpenes and sterols content ≧ 1.0%, 90% of the ultrafine powder in the Ganoderma lucidum broken-wall ultrafine powder has a particle size ≦ 30 μm, and there should be no more than 40 μm of microparticles.

[0021] The present application has the following technical effects: after the Ganoderma lucidum thick slices are treated by the processes of spraying moistening, hot-pressing steam heating, ultrasonic treatment and microwave drying, the content of polysaccharide, triterpenes and sterols is increased, and the release rate of active ingredients is also significantly increased. Whether it is ordinary fine powder or ultra-fine powder, the release rate is much faster than that of the corresponding particle size of the Ganoderma lucidum powder without being treated by the present application technology, which is beneficial to improve the effectiveness of Ganoderma lucidum powder for female insomnia patients. The reason is that the present application technology does not convert other components in Ganoderma lucidum into active ingredients, but destroys the structure of the Ganoderma lucidum mycelium cell wall, so that the active ingredients such as polysaccharide, triterpenes and sterols originally existing in Ganoderma lucidum can be easily extracted and released, thus the content of active ingredients is increased when measured. The Ganoderma lucidum without being treated can not completely extract its components due to the hard Ganoderma lucidum mycelium cell wall, so the measured result is lower.

[0022] The method of the present application can loosen and break the Ganoderma lucidum cells, and change the combined polysaccharide into free state, so as to greatly increase the content of Ganoderma lucidum polysaccharide. The broken Ganoderma lucidum broken wall ultra-fine powder can directly release the active ingredients in Ganoderma lucidum in the digestive tract, which is beneficial to improve the effect of ultra-fine powder on female insomnia. DETAILED DESCRIPTION

[0023] (1) The dried Ganoderma lucidum or Ganoderma sinense fruiting body is washed with water and cut into 2-4 mm thick slices. The purpose of this operation is to reduce the time required for the next step of moistening Ganoderma lucidum with water.

[0024] (2) The Ganoderma lucidum thick slices are moistened with distilled water by spraying.

[0025] (3) The moistened Ganoderma lucidum thick slices are placed in a hot-pressing steam cabinet, sealed, vacuumed, and hot-pressing steam is introduced, the pressure in the hot-pressing steam cabinet is controlled to be 105-137 kPa, heated for 2-4 h, and then the hot steam in the hot-pressing steam cabinet is completely released within 2-5 min.

[0026] (4) The heated Ganoderma lucidum thick slices are placed in an ultrasonic processor for ultrasonic treatment, the ultrasonic power is 200-300 W, the frequency is 20-25 kHz, and the time is 60-90 min.

[0027] (5) The Ganoderma lucidum thick slices after ultrasonic treatment are placed in a microwave drying machine for drying, the microwave frequency is controlled to be 2400-2500 MHz, the drying temperature is 60-70℃, and the drying time is 20-30 min.

[0028] (6) The dried Ganoderma lucidum thick slices are placed in a super micro grinder or an air flow grinder to break the cell wall and pulverize into a broken cell wall super micro powder, to obtain a broken cell wall super micro powder of Ganoderma lucidum for female insomnia. The quality requirements of the broken cell wall super micro powder of Ganoderma lucidum include the following contents: moisture content ≦ 17%, water-soluble extract ≧ 3.0%, polysaccharide content ≧ 2.00%, triterpenes and sterols content ≧ 1.0%, and 90% of the super micro powder in the broken cell wall super micro powder has a particle size ≦ 30 μm, and no more than 40 μm of microparticles are allowed.

[0029] Example 1

[0030] The Ganoderma lucidum fruiting bodies are cleaned and cut into extremely thin slices with an average thickness of 0.4 mm, thin slices with an average thickness of 1.5 mm, and thick slices with an average thickness of 3 mm according to the requirements of the slicing under the general processing rules of Chinese Pharmacopoeia. The Ganoderma lucidum slices are collected and weighed to calculate the loss rate. The results show that the loss rates are 28.5% for the extremely thin slices, 16.2% for the thin slices, and 4.3% for the thick slices. Therefore, the thick slices are determined.

[0031] The loss rate = (m0-m) / m0 x 100%. m0 is the mass of the Ganoderma lucidum fruiting bodies input. m is the mass of the Ganoderma lucidum slices obtained. The results are shown in the following table.

[0032] Sheet type Very thin sheet Thin sheet Thick sheet Loss rate (%) 28.5％ 16.2％ 4.3％

[0033] Example 2

[0034] The Ganoderma lucidum fruiting bodies are cleaned and sprayed with distilled water to soak and infiltrate the Ganoderma lucidum (without slicing) and Ganoderma lucidum thick slices (with an average thickness of 3 mm). Every 1 hour, the Ganoderma lucidum is pried open or cut open to observe the soaking and infiltration conditions.

[0035] The results show that the soaking and infiltration time of the Ganoderma lucidum without slicing is ≧ 12 hours, and the soaking and infiltration time of the Ganoderma lucidum thick slices is 2-4 hours. The soaking and infiltration time can be significantly reduced by slicing into thick slices.

[0036] Example 3

[0037] The Ganoderma lucidum fruiting bodies are cleaned and cut into thick slices with an average thickness of 3 mm. Then, the Ganoderma lucidum thick slices (with an average thickness of 3 mm) are soaked and infiltrated with distilled water. The Ganoderma lucidum thick slices are placed in a hot-pressing steam cabinet, sealed, vacuumed, and hot-pressing steam is introduced. The pressure in the hot-pressing steam cabinet is controlled at 100-137 kPa, and the hot-pressing steam is heated for 2 hours. Then, the hot-pressing steam in the hot-pressing steam cabinet is completely released within 3 minutes. After cooling to room temperature, the Ganoderma lucidum thick slices are taken out, and the content of Ganoderma lucidum polysaccharides and the content of triterpenes and sterols are determined according to the methods of Chinese Pharmacopoeia. The results are shown in the following table.

[0038]

[0039] From the above table, the greater the pressure, the more obvious the increase in the content of polysaccharides, triterpenes and sterols in Ganoderma lucidum. The increase is obvious when the pressure is greater than or equal to 105 kPa, but if the pressure is too large, the operation is not safe. Therefore, the pressure is controlled to be 105-137 kPa.

[0040] Example 4

[0041] The fruiting bodies of Ganoderma lucidum were washed and cut into slices with an average thickness of 3 mm. The Ganoderma lucidum slices (average thickness of 3 mm) were then soaked with distilled water. The slices were placed in a hot-steam cabinet, sealed, vacuumed, and hot steam was introduced. The pressure in the hot-steam cabinet was controlled to be 125 kPa, and the slices were heated for 1-6 h. After natural cooling to room temperature, the Ganoderma lucidum slices were taken out, and the content of polysaccharides and the content of triterpenes and sterols were determined according to the method of Chinese Pharmacopoeia. The results are shown in the table below.

[0042] Steam heating time 1 2 4 6 Polysaccharide content (%) 1.33 2.27 2.40 2.39 Triterpenes and sterols (%) 0.67 0.78 0.77 0.79

[0043] From the above table, the longer the steam heating time, the more obvious the increase in the content of polysaccharides, triterpenes and sterols in Ganoderma lucidum. The increase is obvious when the heating time is more than 2 h, but the increase is lower when the heating time is more than 4 h. Therefore, the heating time is determined to be 2-4 h.

[0044] Example 5

[0045] The fruiting bodies of Ganoderma lucidum were washed and cut into slices with an average thickness of 3 mm. The Ganoderma lucidum slices (average thickness of 3 mm) were then soaked with distilled water. The slices were placed in a hot-steam cabinet, sealed, vacuumed, and hot steam was introduced. The pressure in the hot-steam cabinet was controlled to be 125 kPa, and the slices were heated for 4 h. The slices were then cooled by natural cooling to room temperature or by opening the exhaust valve to rapidly release the hot steam in the cabinet within 3 min. The Ganoderma lucidum slices were taken out, and the content of polysaccharides and the content of triterpenes and sterols were determined according to the method of Chinese Pharmacopoeia. The results are shown in the table below.

[0046] Hot-pressing steam release method Natural cooling Fast steam release Polysaccharide content (%) 2.39 3.02 Triterpenes and sterols (%) 0.79 0.91

[0047] From the above table, the faster the hot steam release speed, the more obvious the increase in the content of polysaccharides, triterpenes and sterols in Ganoderma lucidum. The content of active ingredients in Ganoderma lucidum can be significantly increased when the release time is about 3 min.

[0048] Example 6

[0049] The Ganoderma lucidum fruiting bodies were washed clean and cut into slices with an average thickness of 3 mm, and then the Ganoderma lucidum slices were soaked with distilled water. The slices were placed in a hot-steam cabinet, sealed, vacuumed, and hot-steam was introduced, the pressure in the hot-steam cabinet was controlled at 125 kPa, and heated for 4 h. The vent valve was opened, and the hot-steam in the cabinet was quickly released within 3 min, and then the Ganoderma lucidum slices were cooled and taken out and placed in an ultrasonic processor for ultrasonic treatment. The ultrasonic treatment conditions were as follows: power 250 W, frequency 25 kHz, and sampling every 30 min. The content of Ganoderma lucidum polysaccharides and the content of triterpenes and sterols were determined according to the method in Chinese Pharmacopoeia.

[0050] The results are shown in the following table

[0051]

[0052] From the above table, it can be seen that ultrasonic treatment can increase the content of active polysaccharide components in Ganoderma lucidum. When the ultrasonic treatment time is more than 60 min, the increase is more obvious, and when the ultrasonic treatment time is more than 90 min, the increase is not obvious. Therefore, the ultrasonic treatment time is determined to be 60-90 min.

[0053] Example 7

[0054] The Ganoderma lucidum fruiting bodies were washed clean and cut into slices with an average thickness of 3 mm, and then the Ganoderma lucidum slices were soaked with distilled water. The slices were placed in a hot-steam cabinet, sealed, vacuumed, and hot-steam was introduced, the pressure in the hot-steam cabinet was controlled at 125 kPa, and heated for 4 h. The vent valve was opened, and the hot-steam in the cabinet was quickly released within 3 min, and then the Ganoderma lucidum slices were cooled and taken out and placed in an ultrasonic processor for ultrasonic treatment. The ultrasonic treatment conditions were as follows: power 250 W, frequency 25 kHz, and sampling every 30 min. The content of Ganoderma lucidum polysaccharides and the content of triterpenes and sterols were determined according to the method in Chinese Pharmacopoeia. The results are shown in the following table.

[0055] Drying method Natural air drying Oven drying Microwave drying Polysaccharide content (%) 3.58 3.61 4.12 Triterpenes and sterols (%) 0.94 0.91 1.18

[0056] From the above table, it can be seen that compared with ordinary oven drying and natural air drying, the active ingredient content of the Ganoderma lucidum slices after microwave drying is higher.

[0057] Example 8

[0058] The Ganoderma lucidum fruiting bodies were crushed into fine powder or super fine powder using a super fine crusher.

[0059] Example 9

[0060] The Ganoderma lucidum fruiting body is washed clean and cut into thick pieces with an average thickness of 3 mm, and then the Ganoderma lucidum thick pieces are infiltrated with distilled water. They are placed in a hot-pressing steam cabinet, sealed, vacuumed, and hot-pressing steam is introduced, the pressure in the hot-pressing steam cabinet is controlled to be 125 kPa, heated for 4 h, the exhaust valve is opened, and the hot-pressing steam in the cabinet is quickly released within 3 min, cooled, and the Ganoderma lucidum thick pieces are taken out and placed in an ultrasonic processor for ultrasonic treatment, the ultrasonic treatment conditions are: power 250 W, frequency 25 kHz, and time 60 min. Then they are dried in a microwave drying cabinet, the microwave frequency is 2450 MHz, the drying temperature is 65 ℃, and the drying time is 20 min. Then they are crushed into fine powder (passed through an 80-mesh sieve) and crushed into ultra-fine powder by an ultra-fine pulverizer.

[0061] The particle sizes of the powders of Example 8 and Example 9 are tested. According to the second method (paddle method) of 2020 edition of Chinese Pharmacopoeia Vol. 4 0931 Dissolution and Release Rate Determination Method, the release rates (relative to the content in Ganoderma lucidum) of polysaccharides, triterpenes and sterols released for 30 min are determined, and the release liquid is artificial gastric juice. The test results are shown in the following table.

[0062]

[0063] As shown in the above table, in Example 9, after the Ganoderma lucidum thick pieces are treated by spraying infiltration, hot-pressing steam heating, ultrasonic treatment and microwave drying, not only the contents of polysaccharides, triterpenes and sterols are increased, but also the release rates of active ingredients are significantly improved. Whether it is ordinary fine powder or ultra-fine powder, its release rate is much faster than that of Ganoderma lucidum powder without being treated by the technology of the present application, which is beneficial to improve the effective rate of Ganoderma lucidum powder for female insomnia patients.

[0064] The standard of Ganoderma lucidum powder for female insomnia is determined by measuring a plurality of batches of ultra-fine powder prepared by the technology of the present application.

[0065] The technology of the present application improves the content of active ingredients in Ganoderma lucidum. The reason is not that other components in Ganoderma lucidum are converted into active ingredients by the technology of the present application, but that the structure of the mycelium cell wall of Ganoderma lucidum is destroyed by the technology of the present application, so that the active ingredients originally existing in Ganoderma lucidum can be easily extracted and released, so that the content is increased when measured. The Ganoderma lucidum without being treated can not completely extract its components when measuring the content of active ingredients because the mycelium cell wall of Ganoderma lucidum is hard, so the measured result is lower.

[0066] Example 10

[0067] (1) The dried Ganoderma lucidum fruiting body is washed with water, and cut into Ganoderma lucidum thick pieces with an average thickness of 3 mm.

[0068] (2) The Ganoderma lucidum thick pieces are infiltrated with distilled water by spraying.

[0069] (3) Put the soaked Ganoderma lucidum slices into a hot-steam cabinet, seal, vacuumize, and introduce hot steam. Control the pressure in the hot-steam cabinet to be 110 kPa, and heat treat for 2 h. Then completely release the hot steam in the hot-steam cabinet within 5 min, and cool.

[0070] (4) Put the heat-treated Ganoderma lucidum slices into an ultrasonic processor, and perform ultrasonic treatment. The ultrasonic power is 250 W, the frequency is 25 kHz, and the time is 60 min.

[0071] (5) Put the ultrasonic-treated Ganoderma lucidum slices into a microwave drying machine for drying. Control the microwave frequency to be 2450 MHz, the drying temperature to be 60°C, and the drying time to be 20 min.

[0072] (6) Put the dried Ganoderma lucidum slices into a super-micro pulverizer to be pulverized into super-micro powder, to obtain the Ganoderma lucidum broken-wall super-micro powder for female insomnia.

[0073] Example 11

[0074] (1) Wash the dried Ganoderma lucidum fruiting bodies with water, and cut them into Ganoderma lucidum slices with an average thickness of 3 mm.

[0075] (2) Use a spraying method to spray distilled water on the Ganoderma lucidum slices for soaking.

[0076] (3) Put the soaked Ganoderma lucidum slices into a hot-steam cabinet, seal, vacuumize, and introduce hot steam. Control the pressure in the hot-steam cabinet to be 110 kPa, and heat treat for 2 h. Then completely release the hot steam in the hot-steam cabinet within 5 min, and cool.

[0077] (4) Put the heat-treated Ganoderma lucidum slices into an ultrasonic processor, and perform ultrasonic treatment. The ultrasonic power is 250 W, the frequency is 25 kHz, and the time is 70 min.

[0078] (5) Put the ultrasonic-treated Ganoderma lucidum slices into a microwave drying machine for drying. Control the microwave frequency to be 2450 MHz, the drying temperature to be 60°C, and the drying time to be 20 min.

[0079] (6) Put the dried Ganoderma lucidum slices into a super-micro pulverizer to be pulverized into super-micro powder, to obtain the Ganoderma lucidum broken-wall super-micro powder for female insomnia.

[0080] Example 12

[0081] (1) Wash the dried Ganoderma lucidum fruiting bodies with water, and cut them into Ganoderma lucidum slices with an average thickness of 3 mm.

[0082] (2) Use a spraying method to spray distilled water on the Ganoderma lucidum slices for soaking.

[0083] (3) Put the soaked Ganoderma lucidum thick slices into a hot-pressing steam cabinet, seal, vacuumize, and introduce hot-pressing steam. Control the pressure in the hot-pressing steam cabinet to be 125 kPa, and heat treat for 3 h. Then completely release the hot steam in the hot-pressing steam cabinet within 3 min, and cool.

[0084] (4) Put the heat-treated Ganoderma lucidum thick slices into an ultrasonic processor, and perform ultrasonic treatment. The ultrasonic power is 250 W, the frequency is 25 kHz, and the time is 70 min.

[0085] (5) Put the ultrasonic-treated Ganoderma lucidum thick slices into a microwave drying machine for drying. Control the microwave frequency to be 2450 MHz, the drying temperature to be 70 ℃, and the drying time to be 20 min.

[0086] (6) Put the dried Ganoderma lucidum thick slices into a super-micro pulverizer to be pulverized into super-micro powder, thereby obtaining Ganoderma lucidum broken-wall super-micro powder for female insomnia.

[0087] Example 13

[0088] (1) Wash dried Ganoderma lucidum fruiting bodies with water, and cut them into Ganoderma lucidum thick slices with an average thickness of 3 mm.

[0089] (2) Use a spraying method to spray distilled water on the Ganoderma lucidum thick slices for soaking.

[0090] (3) Put the soaked Ganoderma lucidum thick slices into a hot-pressing steam cabinet, seal, vacuumize, and introduce hot-pressing steam. Control the pressure in the hot-pressing steam cabinet to be 125 kPa, and heat treat for 4 h. Then completely release the hot steam in the hot-pressing steam cabinet within 3 min, and cool.

[0091] (4) Put the heat-treated Ganoderma lucidum thick slices into an ultrasonic processor, and perform ultrasonic treatment. The ultrasonic power is 250 W, the frequency is 25 kHz, and the time is 90 min.

[0092] (5) Put the ultrasonic-treated Ganoderma lucidum thick slices into a microwave drying machine for drying. Control the microwave frequency to be 2450 MHz, the drying temperature to be 70 ℃, and the drying time to be 20 min.

[0093] (6) Put the dried Ganoderma lucidum thick slices into a super-micro pulverizer to be pulverized into super-micro powder, thereby obtaining Ganoderma lucidum broken-wall super-micro powder for female insomnia.

[0094] Comparative Example 1

[0095] (1) Wash dried Ganoderma lucidum fruiting bodies with water, and cut them into Ganoderma lucidum thick slices with an average thickness of 3 mm. Dry the Ganoderma lucidum thick slices.

[0096] (2) The thick slices of Ganoderma lucidum are put into a super-micro pulverizer to be pulverized into super-micro powder to obtain the broken-wall super-micro powder of Ganoderma lucidum.

[0097] The component test results of the broken-wall super-micro powder of Ganoderma lucidum are shown in the following table.

[0098]

[0099] From the above table, it can be seen that in Examples 10-13, after the thick slices of Ganoderma lucidum are treated by spraying moistening, hot-pressing steam heating, ultrasonic treatment and microwave drying, the contents of the extract, polysaccharide, triterpenes and sterols are obviously higher than those in Comparative Example 1.

[0100] Ganoderma lucidum belongs to the Chinese medicine for both medicine and food published by the National Health and Family Planning Commission of China and can be used as food. Therefore, the effects of Ganoderma lucidum powder on 50 female insomnia persons (perimenopausal period, 45-55 years old, divided into 5 groups, 10 persons in each group, 4 groups taking the broken-wall super-micro powder of Ganoderma lucidum treated by the technology of the present application, and 1 group taking the broken-wall super-micro powder of Ganoderma lucidum not treated by the technology of the present application) are observed. 20 persons all show insomnia. The medication method is as follows: Ganoderma lucidum powder is taken with warm water in the morning and evening, 3.0 g each time. The control group is the broken-wall super-micro powder of Ganoderma lucidum not treated by the technology of the present application. The curative effect judgment standard is as follows:

[0101] Cure: sleep time returns to normal or sleep time at night is more than 6 hours, sleep is deep, and spirit is full after waking up.

[0102] Marked effect: sleep is obviously improved, sleep time is increased by more than 3 hours, and sleep depth is increased.

[0103] Effectiveness: symptoms are relieved, and sleep time is increased by less than 3 hours.

[0104] Ineffectiveness: insomnia is not obviously improved or is aggravated after treatment.

[0105] The results are shown in the following table.

[0106] Number of cured patients Number of patients showing effect Number of effective patients Number of ineffective patients Total effective rate (%) Example 10 2 3 4 1 90 Example 11 2 3 5 0 100 Example 12 2 4 4 0 100 Example 13 3 4 3 0 100 Comparative Example 1 0 4 2 4 60

[0107] As can be seen from the table, the treatment effects of the Ganoderma lucidum powder treated by the technology of the present application in Examples 1-4 on female insomnia are generally higher than those in the comparative example.

[0108] The above-described examples have described the technical solutions and beneficial effects of the present application in detail, and it should be understood that the above-described examples are only specific embodiments of the present application and are not used to limit the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the present application.

Claims

1. A preparation method of Ganoderma lucidum broken cell wall ultrafine powder for female insomnia, characterized in that, The preparation method is: (1) dry Ganoderma lucidum or Ganoderma sinense fruiting bodies, wash with water, and cut into thick slices; (2) spray distilled water on the thick slices to moisten them; (3) place the moistened thick slices in a hot-pressing steam cabinet, seal, vacuumize, introduce hot-pressing steam, heat-treat, then release the steam, and cool; (4) place the heat-treated thick slices in an ultrasonic processor, and perform ultrasonic treatment; (5) place the ultrasonic-treated thick slices in a microwave drying machine, and dry them; (6) grind the dried thick slices into ultrafine powder to obtain Ganoderma lucidum broken cell ultrafine powder for female insomnia; In the step (3), the pressure in the hot-pressing steam cabinet is controlled to be 105-137 kPa, and the heating treatment time is 2-4 h; In the step (3), the hot steam in the hot-pressing steam cabinet is completely released within 2-5 min after heating treatment; In the step (5), the microwave frequency in the microwave drying machine is 2400-2500 MHz, the drying temperature is 60-70 ℃, and the drying time is 20-30 min; In the step (6), the quality requirements for the Ganoderma lucidum broken cell ultrafine powder include the following contents: moisture content≦17%, water-soluble extractives≧3.0%, polysaccharide content≧2.00%, triterpenes and sterols content≧1.0%, 90% of the ultrafine powder in the Ganoderma lucidum broken cell ultrafine powder has a particle size≦30 μm, and no more than 40 μm microparticles are allowed.

2. The preparation method of ganoderma lucidum broken wall superfine powder for female insomnia according to claim 1, characterized in that, In the step (1), the thickness of the thick slices is 2-4 mm.

3. The preparation method of ganoderma lucidum broken wall superfine powder for female insomnia according to claim 1, characterized in that, In the step (2), the moistening time is 12-24 h.

4. The preparation method of ganoderma lucidum broken wall superfine powder for female insomnia according to claim 1, characterized in that, In the step (4), the ultrasonic treatment conditions are power 200-300 W, frequency 20-25 kHz, and time 60-90 min.

5. The preparation method of ganoderma lucidum broken wall superfine powder for female insomnia according to claim 1, characterized in that, In the step (6), the dried thick slices are placed in an ultrafine grinder or an airflow grinder to be broken and ground into broken cell ultrafine powder.

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