Bionic myocardial device based on photo-thermal conversion and thermoelectric conversion

Abstract

The invention relates to the technical field of high polymer materials, in particular to a preparation method of a bionic myocardial device based on photo-thermal conversion and thermoelectric conversion, and the bionic myocardial device comprises a thermoelectric gel (EpH) and photo-thermal patch (PHP) double-layer structure. Firstly, a conjugated polymer (HPAE-Py) is synthesized, the conjugated polymer (HPAE-Py), gelatin and Fe < 3 + > are uniformly stirred to prepare PHP, and the PHP has strong wet adhesion and conductive characteristics and can respond to NIR-II (1064nm) in vivo to generate heat. Meanwhile, spiral peptide is designed, conductive polypeptide hydrogel is prepared to serve as a basic framework, a redox conjugate Fe (CN) 64- / Fe (CN) 63-is dispersed in the hydrogel to prepare PHP, and the thermocurrent effect is generated through the temperature difference of different layers of hydrogel. In conclusion, the bionic myocardial device prepared by combining the EpH and the PHP has the advantages of strong heart viscosity, high NIR-II responsiveness and in-vivo power generation, overcomes the operability problem of a power generation device at the heart part, and can be used for treating myocardial infarction.

Description

Technical Field

[0001] The present invention relates to the technical field of new biomedical materials, and in particular to a bionic myocardial device based on photothermal conversion and thermoelectric conversion. Background Art

[0002] Myocardial infarction (MI) is one of the diseases with the highest morbidity and mortality in the world, which seriously threatens human health. After MI, a large number of cardiomyocytes die, which leads to scar formation in the infarct area and its surroundings, resulting in abnormal electrical signal conduction and asynchronous heart contraction. The non-conductivity of the scar electrically uncouples the surviving cardiomyocytes in the infarct area, eventually leading to ventricular dysfunction.

[0003] In vitro studies have shown that electrical stimulation can increase the conduction velocity of cardiomyocytes, change electrophysiological properties, and promote the expression of connexins (Cx40, Cx43). Therefore, introducing electrical properties into biomaterials to prepare bionic myocardium and induce myocardial regeneration may be an effective method for treating myocardial infarction. However, it is difficult to install an electrical generation system in the body to generate electrical stimulation. The low biocompatibility of traditional electrical generation systems and the special physiological environment of the heart further limit their clinical application.

[0004] In summary, in view of the biological characteristics of heart beat and the pathological characteristics of myocardial infarction, it is necessary to control and endow bionic myocardial devices with some corresponding physical and chemical properties, such as softness, viscosity, conductivity and power generation performance. Summary of the invention

[0005] Purpose of the invention: In order to solve the problems existing in the prior art, the present invention provides a bionic myocardial device based on photothermal conversion and thermoelectric conversion, which can respond to NIR-II (1064nm) in vivo, drive the photothermal agent deep in the body to produce heat, achieve a controllable temperature difference, and then use Fe(CN)6 4– / Fe(CN)6 3– (Abbreviation: Fe(CN)6 4- / 3- ) realizes thermoelectric conversion and generates electrical stimulation, overcoming the operability problem of power-generating devices in the heart area and treating myocardial infarction.

[0006] In order to solve the above technical problems, the technical solutions provided by the present invention are as follows:

[0007] A bionic myocardium device based on photothermal conversion and thermoelectric conversion comprises a photothermal layer for converting light into heat and a thermoelectric layer for converting heat into electricity.

[0008] As an improvement, the thermoelectric layer is a thermoelectric gel, which includes a basic skeleton and a thermoelectric conversion system.

[0009] As an improvement, the basic skeleton is a conductive alpha-helical polypeptide with a sequence of HLAUAFEFLAQAVE.

[0010] As an improvement, the thermoelectric conversion system is Fe(CN)6 4- / 3- system.

[0011] As an improvement, the photothermal layer is a photothermal patch, and the photothermal patch is loaded with a photothermal agent.

[0012] The preparation method of the present invention comprises the following steps:

[0013] S1. Preparation of Thermoelectric Gel (EpH)

[0014] S1-1. Preparation of basic skeleton

[0015] An alpha helical peptide, HLAUAFEFLAQAVE, was synthesized by solid phase synthesis, and then the peptide was weighed and dissolved in ultrapure water, and allowed to stand to form a conductive peptide hydrogel, which constitutes the basic skeleton of the thermoelectric gel;

[0016] S1-2. Preparation of thermoelectric conversion system

[0017] Preparation of Fe(CN)6 using ultrapure water as solvent 4- / 3- system, and then disperse it into the basic skeleton obtained in S1-1; use a mold to fix the shape to prepare a thermoelectric gel (EpH);

[0018] S2. Preparation of Photothermal Patch (PHP)

[0019] S2-1. Preparation of photothermal agent

[0020] The conjugated polymer HPAE-Py was synthesized using a two-step Michael synthesis reaction:

[0021] The first step is Michael synthesis: PETA, dopamine hydrochloride, and PEGDA are weighed in proportion and fully dissolved in DMSO, and TEA is used to adjust the pH to 8; then the reaction is stirred at 70-80° C. in the dark; after the reaction is completed, it is cooled to room temperature, filtered, and extracted with MTBE, and the upper liquid is discarded after standing to obtain a polymer; the polymer is rotary evaporated to remove excess purification agent to obtain a hyperbranched polyurethane (HPAE) with a terminal C=C;

[0022] The second step is Michael synthesis: pyrrole is added to HPAE and dissolved evenly in DMSO; TEA is used to adjust the pH to 8, and then the reaction is stirred at 70-80°C in the dark. After the reaction is completed, the pure HPAE-Py polymer is obtained by filtering, purification, and rotary evaporation, and stored in the dark at -20°C for later use;

[0023] S2-2. Preparation of photothermal patch

[0024] Gelatin was uniformly dissolved in ultrapure water, HPAE-Py polymer was added, mixed evenly, and then FeCl3 was added to initiate gelation, and the shape was fixed in a mold to prepare a photothermal patch (PHP);

[0025] S3. Combination

[0026] The EpH obtained from S1 and the PHP obtained from S2 were combined to prepare the bionic myocardial device EpH-PHP.

[0027] As an improvement, the S1-2 step uses ultrapure water to prepare 4 / 4M to 12 / 12M Fe(CN)6 4- / 3- system, and make Fe(CN)6 4- / 3- The final concentration is 0.2 / 0.2M~0.6 / 0.6M.

[0028] As an improvement, the amount of the raw materials in step S2-1 is: weigh 5.96 g PETA, 7.6 g dopamine hydrochloride and 21 g PEGDA and fully dissolve them in 80 mL DMSO.

[0029] As an improvement, the light-protected stirring reaction of the first step Michael synthesis and the second step Michael synthesis in step S2-1 are both carried out in an oil bath at 80°C;

[0030] The time for stirring the reaction in the dark in the first step of Michael synthesis is 2 hours, and the time for stirring the reaction in the dark in the second step of Michael synthesis is 5 hours.

[0031] As an improvement, the amount of the raw materials in step S2-2 is: weigh 7.5 mg of gelatin and dissolve it evenly in 45 μL of ultrapure water, add 30-90 mg of HPAE-Py polymer and mix evenly, and then add 5 μL (final concentration 200 mM) FeCl3 to initiate gelation.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] 1. The present invention includes a photothermal layer that converts light into heat and a thermoelectric layer that converts heat into electricity. The photothermal layer can respond to near-infrared light (NIR), convert the near-infrared light into heat, and then generate electricity through the temperature difference on both sides of the thermoelectric layer. The NIR responded by the photoelectric layer can achieve deep penetration of the heart and perform responsive and controllable heat and electricity generation in the body. This effectively stimulates the implanted site electrically and improves the function of myocardial cells.

[0034] 2. The power generation system used in myocardial infarction needs to adapt to the special physiological characteristics of the heart being deep in the body and having a beating heart, achieve deep tissue penetration and control the temperature difference, and continuously generate current. The present invention designs α-helical polypeptides to prepare conductive polypeptide hydrogels by self-assembly, and introduces Fe(CN)6 4 – / Fe(CN)6 3 – The system gives it the ability of thermoelectric conversion. Through the adhesive conductive heart patch, photothermal conversion is carried out under the deep penetration of NIR-Ⅱ to produce a controllable temperature difference. The combination of the two can achieve photoelectric conversion.

[0035] 3. The bionic myocardial device designed in the present invention has the advantages of simple preparation, easy operation, and low raw material price, and overcomes the existing problems of operability and safety of power generation devices in the heart area. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 Schematic diagram of the structure of a bionic myocardial device based on photothermal conversion and thermoelectric conversion.

[0037] Figure 2 (a) TEM image and (b) SEM image of the polypeptide; (c) circular dichroism spectrum and (d) Fourier transform infrared spectrum of the polypeptide.

[0038] Figure 3 (a) TEM image and (b) SEM image of EpH.

[0039] Figure 4 EpH(Fe(CN)6 4- / 3- CV curves of the concentrations of 0.2 / 0.2M, 0.4 / 0.4M, and 0.6 / 0.6M respectively.

[0040] Figure 5 EpH(Fe(CN)6 4- / 3- EIS curves of the concentrations are 0.2 / 0.2M, 0.4 / 0.4M, and 0.6 / 0.6M respectively.

[0041] Figure 6 This is a macroscopic comparison photo of the HPAE-Py before and after gelation when the concentration is 60%.

[0042] Figure 7 It is the viscoelasticity of the gel when the HPAE-Py concentration is 60%.

[0043] Figure 8The photothermal conversion performance of PHP (HPAE-Py concentrations of 20%, 40%, and 60%, respectively): (a) schematic diagram of the experimental operation; (b) temperature change diagram under direct laser irradiation; (c) temperature change diagram under laser irradiation with the mouse chest cavity blocked; (d) temperature change diagram under laser irradiation with the rat chest cavity blocked.

[0044] Fig. 9 This is the relationship between voltage, current and power during the discharge process of EpH-PHP.

[0045] Fig.10 (a) CV curve and (b) EIS curve of EpH-PHP after twisting and stretching. DETAILED DESCRIPTION

[0046] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments.

[0047] Example 1

[0048] like Figure 1 As shown, this embodiment discloses a bionic myocardium device based on photothermal conversion and thermoelectric conversion, including a photothermal layer that converts light into heat and a thermoelectric layer that converts heat into electricity.

[0049] The thermoelectric layer is the thermoelectric gel EpH, and the photothermal layer is the photothermal patch PHP.

[0050] The manufacturing method of this embodiment includes the following steps:

[0051] S1. Preparation of Thermoelectric Gel (EpH)

[0052] S1-1. Preparation of basic skeleton

[0053] An alpha helical peptide was designed and synthesized by solid phase synthesis as a conductive helical peptide, and the sequence was HLAUAFEFLAQAVE. 2.5 mg of the peptide was weighed and dissolved in 45 μL of ultrapure water, and allowed to stand to form a conductive peptide hydrogel, which constituted the basic skeleton of EpH.

[0054] like Figure 2 As shown in the TEM and SEM images, it can be seen that the polypeptide hydrogel is composed of mesh fibers forming a grid structure, showing a common porous gel microstructure. The circular dichroism spectrum shows that the polypeptides in aqueous solution have a positive absorption peak at 192nm and a broad negative absorption at 228nm, corresponding to the α-helical structure in the n-π* region. In the Fourier transform infrared spectrum, the absorption peaks of amide zone I, zone II, and zone III appear at 1650cm -1 , 1536cm-1 and 1386.85cm -1 At all sites, it was shown that the polypeptide exhibited an α-helical structure.

[0055] S1-2. Preparation of thermoelectric conversion system

[0056] Prepare 4 / 4M Fe(CN)6 using ultrapure water as solvent 4- / 3- system, 5 μL was taken from it and dispersed into the conductive polypeptide gel to make Fe(CN)6 4- / 3- The final concentration was 0.2 / 0.2 M. EpH was prepared using a mold with a fixed shape (cylindrical shape with a diameter of 8 mm).

[0057] like Figure 3 As shown in the TEM and SEM images, compared with the smooth grid surface of the polypeptide hydrogel, the addition of Fe(CN)6 4- / 3- After that, the grid surface is bundled and formed by the aggregation of filaments. The voltammetric characteristic curve of EpH can be surrounded by a certain area of ​​the graph, with obvious redox peaks. Figure 4-5 As shown, with the Fe(CN)6 4- / 3- As the concentration increases, the height of the redox peak gradually increases. The Nyquist plot consists of a semicircle in the front half and a straight line in the back half. 4- / 3- The higher the concentration, the smaller the semicircle diameter, indicating that the conductivity of EpH is: 0.6 / 0.6M>0.4 / 0.4M>0.2 / 0.2M. The output voltage and current of EpH under a temperature difference of 4°C were tested using a multimeter to examine its thermoelectric conversion performance. As shown in Table 1, under the same conditions, the higher the ion concentration, the larger the diameter, and the output voltage and current also increase accordingly.

[0058] Table 1 Different Fe(CN)6 4- / 3- Output voltage and current of EpH of concentration and diameter at 4℃ temperature difference

[0059]

[0060] S2. Preparation of Photothermal Patch (PHP)

[0061] S2-1. Preparation of photothermal agent

[0062] HPAE-Py polymer was synthesized by two-step Michael addition reaction. Step 1: Weigh 5.96g PETA, 7.6g dopamine hydrochloride and 21g PEGDA and fully dissolve them in 80ml DMSO. TEA will adjust the pH value to about 8. The solution was transferred to a single-necked flask and stirred in an oil bath at 80℃ for 2h in the dark. After the reaction was completed, it was cooled to room temperature and filtered in a glass conical funnel plugged with cotton. MTBE was extracted three times, each time for 15min. After standing, the upper liquid was discarded to obtain a liquid, light yellow polymer in the lower layer. Finally, the polymer was rotary evaporated to remove excess purification agent to obtain a hyperbranched polyurethane (HPAE) with C=C at the end. Step 2: Weigh 5 g of pyrrole, add the HPAE prepared above and dissolve evenly in DMSO, adjust the pH value to about 8 with TEA, and react in an 80°C oil bath in the dark for 5 h with stirring. After the reaction is completed, filter, purify and rotary evaporate to obtain pure HPAE-Py polymer, and store in the dark at -20°C for later use.

[0063] S2-2. Preparation of photothermal patch

[0064] Weigh 7.5 mg of gelatin and dissolve it in 45 μL of ultrapure water, add 90 mg of HPAE-Py polymer and mix well. Finally, add 5 μL (final concentration 200 mM) of FeCl3 to initiate gelation. Prepare PHP using a mold to fix the shape (cylindrical with a diameter of 8 mm).

[0065] like Figure 6-7 As shown in the figure, when the concentration of HPAE-Py is 60%, it can quickly gel, has strong viscosity before curing, and has strong elasticity after curing. In order to study the photothermal effect of PHP, a commercially available 1064nm near-infrared laser was used to continuously irradiate PHP of different concentrations under different conditions, and an infrared thermal imager monitored the temperature changes of PHP under irradiation. Figure 8 As shown in the photothermal experiment operation diagram in a, PHP is placed in a dish for experiment, a mouse chest of appropriate size is taken, PHP is attached to the inside, the height of the laser is kept consistent, and the temperature of PHP is recorded by the infrared thermal imager at regular intervals. The results show that when PHP is directly and continuously irradiated by NIR-II (1064nm, 1W), the temperature rises rapidly. The higher the concentration, the faster and higher the temperature rises. When the HPAE-Py concentration is 60%, the highest temperature that can be detected by the thermal imager is 150℃ at t=5min. When PHP is blocked by the mouse chest, the temperature can still rise steadily. At t=5min, the PHP temperature is about 42℃. When PHP is blocked by the rat chest, the temperature rise rate decreases significantly. At t=5min, the temperature is about 41℃. When the laser irradiation time is extended, when t=8min, the temperature is about 42℃.

[0066] S3. Combination

[0067] The EpH obtained from S1 and the PHP obtained from S2 were combined to prepare the bionic myocardial device EpH-PHP.

[0068] like Figure 1 As shown, Figure 1 -a is the overall schematic diagram of the bionic myocardial device and its working schematic diagram in the heart area. Figure 1 -b is the photothermal conversion diagram of PHP, Figure 1 -c is a schematic diagram of the design, assembly and preparation of EpH.

[0069] In vitro study of its photoelectric conversion properties: EpH was assembled into a sandwich structure Cu|EpH|Cu with copper foil of a certain thickness so that the output voltage and current could be detected by a multimeter. PHP was placed on the other side of the copper foil facing EpH. NIR-II (1064nm, 1W) was used to irradiate PHP for 8 minutes to charge it. The laser was removed and the current-voltage curve was obtained using a multimeter during the discharge process to obtain the output power. Fig. 9 As shown in the figure, under a temperature difference of 4°C, the corresponding power curve of the EpH-PHP discharge process shows a parabolic behavior, with a maximum value of about 4.3mW and a maximum current of about 20μA. The electrochemical performance stability of EpH-PHP after twisting and stretching was studied by the classical two-electrode method, as shown in the figure. Fig.10 As shown, compared with the control group without any treatment, the CV curves of EpH-PHP after stretching and twisting are almost the same, and the EIS radius is also almost the same, indicating that the energy storage properties and resistance are almost unchanged. Regardless of whether EpH-PHP is stretched or twisted, its electrochemical properties are relatively stable.

[0070] Example 2

[0071] The preparation method of this example includes the following steps:

[0072] S1. Preparation of Thermoelectric Gel (EpH)

[0073] S1-1. Preparation of basic skeleton

[0074] An alpha helical peptide was designed and synthesized by solid phase synthesis as a conductive helical peptide, and the sequence was HLAUAFEFLAQAVE. 1.25 mg of the peptide was weighed and dissolved in 45 μL of ultrapure water, and allowed to stand to form a conductive peptide hydrogel, which constituted the basic skeleton of EpH.

[0075] S1-2. Preparation of thermoelectric conversion system

[0076] Use ultrapure water as solvent to prepare 8 / 8M Fe(CN)6 4- / 3- 5 μL of it was dispersed in the conductive polypeptide gel to make Fe(CN)6 4- / 3-The final concentration was 0.4 / 0.4 M. EpH was prepared using a mold with a fixed shape (cylindrical shape with a diameter of 7 mm).

[0077] S2. Preparation of Photothermal Patch (PHP)

[0078] S2-1. Preparation of photothermal agent

[0079] HPAE-Py polymer was synthesized by two-step Michael addition reaction. Step 1: Michael addition: 5.96g PETA, 7.6g dopamine hydrochloride and 21g PEGDA were weighed and fully dissolved in 80ml DMSO, and TEA was used to adjust the pH value to about 8. The solution was transferred to a single-necked flask and stirred in an oil bath at 80℃ for 2h in the dark. After the reaction was completed, it was cooled to room temperature and filtered in a glass conical funnel plugged with cotton. MTBE was extracted three times, each time for 15min, and the upper layer of liquid was discarded after standing to obtain a liquid, light yellow polymer in the lower layer. Finally, the polymer was rotary evaporated to remove excess purification agent to obtain a hyperbranched polyurethane (HPAE) with C=C at the end. Step 2: Weigh 5 g of pyrrole, add the HPAE prepared above and dissolve evenly in DMSO, adjust the pH value to about 8 with TEA, and react in an 80°C oil bath in the dark for 5 h with stirring. After the reaction is completed, filter, purify and rotary evaporate to obtain pure HPAE-Py polymer, and store in the dark at -20°C for later use.

[0080] S2-2. Preparation of photothermal patch

[0081] Weigh 7.5 mg of gelatin and dissolve it in 45 μL of ultrapure water, add 60 mg of HPAE-Py polymer and mix well. Finally, add 5 μL (final concentration 200 mM) of FeCl3 to initiate gelation. Use a mold to fix the shape (cylindrical with a diameter of 7 mm).

[0082] The EpH obtained from S1 and the PHP obtained from S2 were combined to prepare the bionic myocardial device EpH-PHP.

[0083] Example 3

[0084] The preparation method of this example includes the following steps:

[0085] S1. Preparation of Thermoelectric Gel (EpH)

[0086] S1-1. Preparation of basic skeleton

[0087] An alpha helical peptide was designed and synthesized by solid phase synthesis as a conductive helical peptide, and the sequence was HLAUAFEFLAQAVE. 2 mg of the peptide was weighed and dissolved in 45 μL of ultrapure water, and allowed to stand to form a conductive peptide hydrogel, which constituted the basic skeleton of EpH.

[0088] S1-2. Preparation of thermoelectric conversion system

[0089] Use ultrapure water as solvent to prepare 12 / 12M Fe(CN)6 4- / 3- 5 μL of it was dispersed in the conductive polypeptide gel to make Fe(CN)6 4- / 3- The final concentration was 0.6 / 0.6 M. EpH was prepared using a mold with a fixed shape (cylindrical shape with a diameter of 6 mm).

[0090] S2. Preparation of Photothermal Patch (PHP)

[0091] S2-1. Preparation of photothermal agent

[0092] HPAE-Py polymer was synthesized by two-step Michael addition reaction. Step 1: Michael addition: 5.96g PETA, 7.6g dopamine hydrochloride and 21g PEGDA were weighed and fully dissolved in 80ml DMSO, and TEA was used to adjust the pH value to about 8. The solution was transferred to a single-necked flask and stirred in an oil bath at 80℃ for 2h in the dark. After the reaction was completed, it was cooled to room temperature and filtered in a glass conical funnel plugged with cotton. MTBE was extracted three times, each time for 15min, and the upper layer of liquid was discarded after standing to obtain a liquid, light yellow polymer in the lower layer. Finally, the polymer was rotary evaporated to remove excess purification agent to obtain a hyperbranched polyurethane (HPAE) with C=C at the end. Step 2: Weigh 5 g of pyrrole, add the HPAE prepared above and dissolve evenly in DMSO, adjust the pH value to about 8 with TEA, and react in an 80°C oil bath in the dark for 5 h with stirring. After the reaction is completed, filter, purify and rotary evaporate to obtain pure HPAE-Py polymer, and store in the dark at -20°C for later use.

[0093] S2-2. Preparation of photothermal patch

[0094] Weigh 7.5 mg of gelatin and dissolve it in 45 μL of ultrapure water, add 30 mg of HPAE-Py polymer and mix well. Finally, add 5 μL (final concentration 200 mM) of FeCl3 to initiate gelation. Prepare PHP using a mold to fix the shape (cylindrical with a diameter of 6 mm).

[0095] S3. Combination

[0096] The EpH obtained from S1 and the PHP obtained from S2 were combined to prepare the bionic myocardial device EpH-PHP.

[0097] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A bionic myocardial device based on photothermal conversion and thermoelectric conversion, characterized in that: It includes a photothermal layer that converts light into heat and a thermoelectric layer that converts heat into electricity.

2. A bionic myocardium device based on photothermal conversion and thermoelectric conversion according to claim 1, characterized in that: The thermoelectric layer is a thermoelectric gel, which includes a basic skeleton and a thermoelectric conversion system.

3. A bionic myocardium device based on photothermal conversion and thermoelectric conversion according to claim 2, characterized in that: The basic skeleton is a conductive alpha-helical polypeptide with a sequence of HLAUAFEFLAQAVE.

4. The bionic myocardium device based on photothermal conversion and thermoelectric conversion according to claim 2, characterized in that: The thermoelectric conversion system is Fe(CN)6 4– / Fe(CN)6 3– system.

5. The bionic myocardium device based on photothermal conversion and thermoelectric conversion according to claim 1, characterized in that: The photothermal layer is a photothermal patch, and the photothermal patch is loaded with a photothermal agent.

6. A method for preparing a bionic myocardial device according to claim 1, characterized in that: The steps include: S1. Preparation of Thermoelectric Gel (EpH) S1-1. Preparation of basic skeleton The alpha helical polypeptide is synthesized by solid phase synthesis, and then the polypeptide is weighed and dissolved in ultrapure water, and allowed to stand to form a conductive polypeptide hydrogel, which constitutes the basic skeleton of the thermoelectric gel; S1-2. Preparation of thermoelectric conversion system Preparation of Fe(CN)6 using ultrapure water as solvent 4– / Fe(CN)6 3– system, and then disperse it into the basic skeleton obtained in S1-1; use a mold to fix the shape to prepare a thermoelectric gel (EpH); S2. Preparation of Photothermal Patch (PHP) S2-1. Preparation of photothermal agent The conjugated polymer HPAE-Py was synthesized using a two-step Michael synthesis reaction: The first step is Michael synthesis: PETA, dopamine hydrochloride, and PEGDA are weighed in proportion and fully dissolved in DMSO, and TEA is used to adjust the pH to 8; then the reaction is stirred at 70-80° C. in the dark; after the reaction is completed, it is cooled to room temperature, filtered, and extracted with MTBE, and the upper liquid is discarded after standing to obtain a polymer; the polymer is rotary evaporated to remove excess purification agent to obtain a hyperbranched polyurethane (HPAE) with a terminal C=C; The second step is Michael synthesis: pyrrole is added to HPAE and dissolved evenly in DMSO; TEA is used to adjust the pH to 8, and then the reaction is stirred at 70-80°C in the dark. After the reaction is completed, the pure HPAE-Py polymer is obtained by filtering, purification, and rotary evaporation, and stored in the dark at -20°C for later use; S2-2. Preparation of photothermal patch Gelatin was uniformly dissolved in ultrapure water, HPAE-Py polymer was added, mixed evenly, and then FeCl3 was added to initiate gelation, and the shape was fixed in a mold to prepare a photothermal patch (PHP); S3. Combination The EpH obtained from S1 and the PHP obtained from S2 were combined to prepare the bionic myocardial device EpH-PHP.

7. The method for preparing a bionic myocardium device according to claim 6, characterized in that: The S1-2 step uses ultrapure water to prepare 4 / 4M to 12 / 12M Fe(CN)6 4– / Fe(CN)6 3– system, and make Fe(CN)6 4– / Fe(CN)6 3– The final concentration is 0.2 / 0.2M~0.6 / 0.6M.

8. The method for preparing a bionic myocardium device according to claim 6, characterized in that: The amount of the raw materials used in step S2-1 is as follows: 5.96 g PETA, 7.6 g dopamine hydrochloride and 21 g PEGDA are weighed and fully dissolved in 80 mL DMSO.

9. The method for preparing a bionic myocardium device according to claim 6, characterized in that: The light-proof stirring reaction of the first step Michael synthesis and the second step Michael synthesis in step S2-1 were both carried out in an oil bath at 80°C; The time for stirring the reaction in the dark in the first step of Michael synthesis is 2 hours, and the time for stirring the reaction in the dark in the second step of Michael synthesis is 5 hours.

10. The method for preparing a bionic myocardium device according to claim 6, characterized in that: The dosage of the raw materials in step S2-2 is as follows: weigh 7.5 mg of gelatin and dissolve it evenly in 45 μL of ultrapure water, add 30-90 mg of HPAE-Py polymer and mix evenly, and then add 5 μL (final concentration 200 mM) of FeCl3 to initiate gelation.

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