Strain with effects of protecting liver and expelling toxin and application thereof

Abstract

The invention discloses a bacterial strain with effects of protecting liver and expelling toxins and application of the bacterial strain. The bacterial strain is Bifidobacterium animalis BL03, is preserved in Guangdong Microbial Culture Collection Center, and has the preservation number of GDMCC NO.64840. The bacterial strain has the effects of protecting liver and expelling toxins and the application of the bacterial strain. The preservation time is July 8, 2024; the strain with the effects of protecting the liver and expelling toxins can relieve liver injury, can reduce the content of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase in serum, and can achieve the effects of protecting the liver and expelling toxins.

Description

Technical Field

[0001] The present invention relates to the field of microbial technology, and in particular to a strain with liver-protecting and detoxifying effects and an application thereof. Background Art

[0002] Liver damage caused by obesity is mainly due to improper eating habits, lack of exercise, side effects of drugs or alcohol abuse. Bad eating habits will lead to energy intake exceeding the body's needs, and the excess calories will be converted into fat storage, which will cause weight gain and increase the burden on the liver. Improving the dietary structure, reducing the intake of high-fat and high-sugar foods, and increasing the intake of dietary fiber will help alleviate the symptoms of obesity-related liver disease. Lack of exercise makes the body consume less energy than the energy it takes in. This will lead to fat accumulation in the long run, which will affect the function of the liver.

[0003] Probiotics are a type of active microorganisms that are beneficial to the host by colonizing in the human body and changing the composition of the flora in a certain part of the host. They promote nutrient absorption and maintain intestinal health by regulating the host mucosal and systemic immune functions or by regulating the balance of intestinal flora, thus producing single microorganisms or mixed microorganisms with clear composition that are beneficial to health.

[0004] At present, obesity-related liver damage is mainly treated through diet therapy and exercise therapy; diet therapy and exercise therapy need to continue for a certain period of time, so some probiotics can be used to better achieve the purpose of alleviating liver damage. Summary of the invention

[0005] The purpose of the present invention is to provide a strain with liver-protecting and detoxifying effects and its application. The strain of the present invention can reduce the levels of alanine aminotransferase and aspartate aminotransferase in serum, and can achieve the effect of liver-protecting and detoxifying, and the effect is significant.

[0006] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0007] The first aspect of the present invention provides a strain with liver protection and detoxification effects, wherein the strain is Bifidobacterium animalis subsp. lactis BL03, which is preserved in Guangdong Provincial Microbiological Culture Collection Center (5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou), with a preservation number of GDMCC NO.64840; the preservation time is July 8, 2024.

[0008] The second aspect of the present invention is a microbial agent containing the strain having liver-protecting and detoxifying effects.

[0009] Preferably, the microbial agent is obtained by inoculating the strain with liver protection and detoxification effects into a culture medium for cultivation.

[0010] The third aspect of the present invention provides a use of the strain having liver protection and detoxification efficacy or the microbial agent in the preparation of a liver protection and detoxification product.

[0011] The fourth aspect of the present invention provides a product with liver protection and detoxification effects, comprising the strain with liver protection and detoxification effects or the microbial agent.

[0012] Preferably, the number of viable bacteria of the strain with liver protection and detoxification effect is not less than 2×10 8 cfu / g, or the cell count of the microbial agent is not less than 2×10 8 cells / g.

[0013] Compared with the prior art, the beneficial effects of the present invention include at least:

[0014] The strain with liver protection and detoxification efficacy of the present invention can reduce the content of alanine aminotransferase and aspartate aminotransferase in serum, and can achieve the effect of liver protection and detoxification; in addition, the strain with liver protection and detoxification efficacy of the present invention can reduce the cholesterol content in serum, and has the effect of promoting weight loss. The use of the strain with liver protection and detoxification efficacy of the present invention in diet therapy and exercise therapy can achieve the purpose of weight loss more quickly, and can quickly relieve liver damage caused by obesity. DETAILED DESCRIPTION

[0015] The following embodiments of the technical solution of the present invention are described in detail in conjunction with the embodiments. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and are therefore only used as examples, and cannot be used to limit the protection scope of the present invention.

[0016] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the present invention belongs.

[0017] The embodiment of the present invention provides a strain with liver protection and detoxification effects, wherein the strain is Bifidobacterium animalis subsp. lactis BL03, which is preserved in Guangdong Microbiological Culture Collection Center with a preservation number of GDMCC NO.64840; the preservation time is July 8, 2024.

[0018] The strain with liver-protecting and detoxifying effects of the present invention can alleviate liver damage caused by obesity, and can reduce the cholesterol content in serum, and has the effect of promoting weight loss. The use of the strain with liver-protecting and detoxifying effects of the present invention in diet therapy and exercise therapy can achieve the purpose of weight loss more quickly, and can quickly alleviate liver damage caused by obesity.

[0019] Another embodiment of the present invention provides a microbial agent containing the strain having liver-protecting and detoxifying effects.

[0020] In one embodiment, the microbial agent is obtained by inoculating the strain having liver protection and detoxification effects into a culture medium for cultivation.

[0021] Another embodiment of the present invention provides a use of the strain having liver protection and detoxification effects or the microbial agent in the preparation of liver protection and detoxification products.

[0022] In the present invention, the strain with liver protection and detoxification effects has the effect of reducing serum cholesterol and the levels of alanine aminotransferase and aspartate aminotransferase. The strain with liver protection and detoxification effects of the present invention can promote weight loss when used in diet therapy and exercise therapy, and can also alleviate liver damage and achieve the effect of protecting the liver.

[0023] Another embodiment of the present invention provides a product with liver protection and detoxification effects, including the strain with liver protection and detoxification effects or the microbial agent.

[0024] In one embodiment, the viable bacterial count of the strain having liver protection and detoxification effects is not less than 2×10 8 cfu / g. Specifically, in one embodiment, the viable bacterial count of the strain having liver protection and detoxification effects is 2×10 8 cfu / g. In other embodiments, the viable bacterial count of the strain with liver protection and detoxification effect is 5×10 9 cfu / g; in another embodiment, the cell count of the microbial agent is not less than 2×10 8 cells / g.

[0025] In the present invention, by limiting the content of the strain with liver protection and detoxification effects, it can be better ensured that the prepared product has a more excellent effect of alleviating liver damage and losing weight.

[0026] The technical solution of the present invention is further described in detail below through specific embodiments.

[0027] Example 1

[0028] This example is the screening of Bifidobacterium animalis subsp. lactis BL03;

[0029] Several samples of fresh feces from healthy children in Zhongxiang City, Hubei Province were collected using a disposable sterile stool collector, and the samples were applied to LBS culture medium with 1% nystatin after gradient dilution, and enriched and cultured in an anaerobic incubator for 48 hours. After culture, colonies were picked and streaked with an inoculation loop for purification based on the color, size, edge shape, etc. of the colonies. After identification, a strain of Bifidobacterium animalis subsp. lactis BL03 was obtained.

[0030] The morphological identification characteristics of Bifidobacterium animalis subsp. lactis BL03 are as follows: curved and bifurcated under a microscope, the colonies are milky white and convex.

[0031] Example 2

[0032] This example is a study on the functional effects of Bifidobacterium animalis subsp. lactis BL03:

[0033] 1. Obesity model construction:

[0034] DIO mice were fed a high-fat diet for 2 weeks to obtain an obesity model, and then randomly divided into 2 groups, 10 mice in each group;

[0035] 2. Intragastric gavage samples:

[0036] Bifidobacterium animalis subsp. lactis BL03 was prepared with physiological saline to a concentration of 2×10 8 cfu / ml solution;

[0037] 3. Test methods:

[0038] The first group was gavaged with a solution of animal Bifidobacterium BL03, and the second group was gavaged with normal saline. The gavage volume was 1 ml per mouse, once a day for 3 weeks. The weight changes of the mice were recorded. After the third week, blood was collected to test the serum total cholesterol content, as well as the serum alanine aminotransferase and aspartate aminotransferase levels.

[0039] Weight change rate = average weight of mice after the experiment / average weight of mice before the experiment × 100%;

[0040] The test results are shown in Table 1:

[0041] Table 1

[0042] project Group 1 Group 2 Weight change rate 74.6％ 134.2％ Total cholesterol content (mmol / L) 2.3 4.4 Alanine aminotransferase content (U / L) 5.6 8.7 Aspartate aminotransferase content (U / L) 0.3 8.3

[0043] From Table 1, we can see that:

[0044] The animal Bifidobacterium (Bifidobacterium animalis subsp. lactis) BL03 of the present application can reduce the cholesterol and alanine aminotransferase and aspartate aminotransferase levels in serum. It can be seen that the animal Bifidobacterium (Bifidobacterium animalis subsp. lactis) BL03 of the present application has the effect of protecting the liver and can achieve the effect of weight loss.

[0045] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and specification of the present invention.

Claims

1. A strain with liver protection and detoxification effects, characterized in that: The strain is Bifidobacterium animalis subsp. lactis BL03, which is preserved in Guangdong Provincial Microbiological Culture Collection Center with the preservation number GDMCC NO.64840; the preservation time is July 8, 2024.

2. A microbial agent containing the strain with liver protection and detoxification effects as claimed in claim 1.

3. The microbial agent according to claim 2, characterized in that: The microbial agent is obtained by inoculating the strain with liver protection and detoxification effects into a culture medium for cultivation.

4. Use of the strain with liver protection and detoxification effects as claimed in claim 1 or the microbial agent as claimed in claim 2 in the preparation of liver protection and detoxification products.

5. A product with liver protection and detoxification function, characterized in that: It includes the strain with liver protection and detoxification effect as described in claim 1 or the microbial agent as described in claim 2.

6. The product according to claim 5, characterized in that The number of viable bacteria of the strain with liver protection and detoxification effect is not less than 2×10 8 cfu / g, or the cell count of the microbial agent is not less than 2×10 8 cells / g.