Pretreatment method and detection method for detecting medicines in plasma

By using carboxylated modified TiO2@MWCNTs for solid phase dispersion extraction, the problems of cumbersome pre-processing and serious matrix interference in the prior art are solved, efficient and convenient drug detection in plasma is achieved, and detection sensitivity and accuracy are improved.

CN119715874APending Publication Date: 2025-03-28SHENZHEN LONGGANG DISTRICT NO 2 PEOPLES HOSPITAL +1
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Patent Information

Application Number
CN202411770003.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-04
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

In the prior art, when detecting drugs in plasma, the pre-sample treatment process is cumbersome, time-consuming and difficult to effectively reduce matrix interference such as phospholipids, affecting the precision and accuracy of the detection results.

Method used

Carboxylation-modified TiO2@MWCNTs are used as the extraction/adsorption material for solid phase dispersion extraction, interacting with phospholipid components through Lewis acid-base interaction, and using the large specific surface area of ​​multi-walled carbon nanotubes to adsorb other matrix interference substances to reduce ion suppression phenomena and background noise.

Benefits of technology

It significantly reduces matrix interference such as phospholipids, improves the sensitivity and linear detection range in LC-MS/MS detection, simplifies the pre-processing process, and reduces costs.

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Abstract

The invention belongs to the field of drug detection, and particularly discloses a pretreatment method and a detection method for detecting drugs in plasma. The pretreatment method comprises the following steps: mixing a plasma sample containing metformin, aripiprazole or amisulpride micromolecule drugs after protein removal with carboxylated TiO2 (at) MWCNTs, and carrying out solid-phase dispersion extraction to obtain a supernatant which is used as a subsequent LC-MS / MS injection sample for detection. The carboxylation modified TiO2 (at) MWCNTs are used as an extraction / adsorption material for solid-phase dispersion extraction, so that the interference of matrixes such as phospholipid and the like is greatly reduced, the influence of an ion inhibition phenomenon and background noise on the quantification of small molecule drugs in the subsequent detection and analysis process is reduced, the sensitivity in LC-MS / MS detection is improved, and the linear detection range is expanded; in addition, the method is simple, convenient, rapid and low in cost.
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