Monoclonal cell for expressing HPV (human papilloma virus) 18 type E6 protein and application of monoclonal cell in construction of HPV tumor animal model
By constructing and screening monoclonal cells expressing HPV18 E6 protein and establishing an HPV tumor animal model, the instability of polyclonal cell lines and poor repetition of experimental results were solved, efficient gene function research and immune response induction were achieved, and significant application prospects were achieved.
Patent Information
- Application Number
- CN202510273558.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-10
- Publication Date
- 2025-04-04
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing polyclonal cell lines of HPV18-related tumor models have problems such as inconsistent expression levels, inconsistent growth rates, poor stability and poor repeatability of experimental results, making it difficult to conduct accurate gene function research.
Monoclonal cells expressing HPV18 type E6 protein were constructed and screened by limited dilution and single-cell imaging sorting identification, combined with target gene sequencing and RT-PCR verification, and applied to non-human mammals to establish an HPV tumor animal model.
The stable expression of monoclonal cells and the stable growth of tumors have been achieved, the repetition of experimental results and the accuracy of genetic function research are improved, and it can effectively induce animal models to produce high levels of humoral immunity and cellular immune response, and has good application prospects for screening and prevention and/or treatment HPV drug compositions or vaccine compositions.