A method for cell anti-aging
By combining mesenchymal stem cells (MSCs) with metformin, the challenges of cell differentiation pathway regulation, cell survival and function optimization, as well as long-term efficacy and safety in the field of anti-aging are solved, and the effect of significantly enhancing the expression level of GPx7 in cells is achieved, thereby reversing the effect of cellular aging.
Patent Information
- Application Number
- CN202411980478.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-04-08
AI Technical Summary
The prior art has not fully explored its combined use effect in the field of anti-aging when using mesenchymal stem cells (MSCs) and metformin, especially in the precise regulation of cell differentiation pathways, optimized survival and function after cell transplantation, and ensure long-term efficacy and safety.
A method for cell anti-aging is developed by combining umbilical or embryonic mesenchymal stem cells (MSCs) with metformin. The method includes the use of MSCs in the cell aging model and the addition of metformin treatment. The specific steps include cell inoculation, D-galactose-induced senescence, MSCs treatment and metformin addition, and subsequent senescence detection, including β-galactosidase method and GPx7 gene detection.
Experimental results show that the combination of MSCs and metformin can significantly enhance the expression level of glutathione peroxidase 7 (GPx7) in senescent cells, thereby reversing the aging state of cells and reducing the potential inhibitory effect of metformin on cells when used alone, showing better safety and effect.