Bactericidal composition and application thereof

Abstract

The invention relates to a bactericidal composition and application thereof, the bactericidal composition comprises an active component A and an active component B. The active component A is a compound represented by formula (I), and the active component B is any one of bromothalonil and prochloraz. The mass ratio of the active component A to the active component B is (1: 35)-(34: 1). The bactericidal composition or the preparation thereof provided by the invention can improve the control effect, has a remarkable synergistic effect, and can effectively reduce the dosage and delay the development of drug resistance of diseases.

Description

Technical Field

[0001] The present invention relates to the field of pesticide bactericides, and in particular to a bactericidal composition and application thereof. Background Art

[0002] Strobilurin fungicides are a class of low-toxic, broad-spectrum, highly effective, systemic fungicides that have good activity against almost all fungal diseases (Oomycetes, Phycomycetes, Ascomycetes and Deuteromycetes) such as powdery mildew, anthracnose, rust, web blotch, downy mildew, rice blast, etc. The compound of formula (I) is a strobilurin fungicide developed by Sumitomo Chemical Co., Ltd., CAS registration number: 2454319-63-0, and the specific chemical structure is as follows:

[0003]

[0004] The most effective means of preventing and controlling plant diseases is still chemical control, but the long-term use of a single agent can easily lead to a significant increase in the resistance of the disease, thereby greatly increasing the amount of medicine applied, and causing more serious pollution to the environment. Compounding two active ingredients without cross-resistance can greatly delay the improvement of disease resistance, expand the fungicide spectrum, improve drug efficacy, thereby reducing the amount of pesticide used, and thus reducing pollution to the environment. The present invention surprisingly finds that the composition has a significant synergistic effect by mixing the compound of formula (I) with bromothionil and prochloraz, which can significantly improve the control effect of treatment and protection, reduce the amount of medicine used, expand the control spectrum, reduce the control cost, and is safe for crops. Summary of the invention

[0005] Based on the above situation, the purpose of the present invention is to provide a fungicide composition, which is mainly used for preventing and controlling plant fungal diseases. The composition or its preparation can enhance the efficacy, reduce the dosage, and at the same time prolong the lasting effect and delay the development of drug resistance.

[0006] In order to achieve the above object, the following technical solution is provided: a bactericidal composition comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is a compound represented by formula (I), and the chemical structure is: The active ingredient B is any one of bromothionil and prochloraz;

[0007] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 34:1 or any value between the above values;

[0008] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 28:1;

[0009] When the active ingredient B is bromothionil, the mass ratio of the compound of formula (I) to bromothionil is 1:28 to 28:1;

[0010] Furthermore, the mass ratio of the compound of formula (I) to bromothionil is 1:28 to 14:1.

[0011] Further, the mass ratio of the compound of formula (I) to bromothionil is 1:28, 1:14, 1:5, 2:5, 5:1, 14:1;

[0012] When the active ingredient B is prochloraz, the mass ratio of the compound of formula (I) to prochloraz is 1:32 to 26:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to prochloraz is 1:32 to 13:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to prochloraz is 1:32, 1:13, 1:6, 2:3, 6:1, 13:1;

[0015] Furthermore, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 0.5 to 90 wt%;

[0016] Furthermore, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 1 to 80 wt%;

[0017] Furthermore, the fungicide composition contains, in addition to the active ingredient, agriculturally permitted auxiliary ingredients, wherein the auxiliary ingredients are selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist, a binder or a carrier;

[0018] Further, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0019] Further, the dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0020] Further, the emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0021] Further, the thickener is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0022] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0023] Further, the antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0024] Furthermore, the defoaming agent is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C 8 -C 10 One or more of fatty alcohols; and / or

[0025] Further, the solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil (soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives and water; and / or

[0026] Further, the preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0027] Further, the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-methylphenol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomaceous earth, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0028] Further, the synergist is selected from synergist phosphine, piperonyl ether; and / or

[0029] Further, the carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0030] Further, the formulation of the fungicide composition is selected from solid preparations and / or liquid preparations and / or seed treatment preparations;

[0031] The solid preparations include powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0032] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, fats, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions or microcapsule suspension-suspoemulsions;

[0033] Furthermore, the fungicide composition can be prepared into a formulation acceptable to pesticides, and the formulation is a microemulsion, an aqueous emulsion, a suspension, a dispersible oil suspension, a soluble solution, an emulsifiable concentrate, a suspoemulsion, a microcapsule suspension, a water-dispersible granule, a wettable powder, a granule, a seed treatment suspension, or a seed treatment dry powder;

[0034] Furthermore, the formulation is in the form of a microemulsion, emulsifiable concentrate, suspension, aqueous emulsion, water-dispersible granules, wettable powder, or seed treatment suspension.

[0035] The present invention also discloses the use of the above-mentioned fungicide composition in preventing and controlling plant diseases;

[0036] Further, the plant diseases are plant diseases caused by pathogens such as Blumiria, Erysiphe, Gibberella, Sclerotinia, Botryotinia, Puccinia, Tilletia, Gymnosporzngium, Phakopsora, Ustilago, Rhizoctonia, Verticillium, Alternaria, Pyriculara, Botrytis, Cercospora, Fusarium, Colletotrichum, Rhizoctonia, and Sclerotium;

[0037] Furthermore, the plant disease is a plant disease caused by Anthracnose fungi;

[0038] Furthermore, the Colletotrichum fungi include Colletotrichum higginsianum, Colletotrichum orbiculare, Colletotrichum lindemuthianum, Colletotrichum capsici, and Colletotrichum gloeosporioides;

[0039] Furthermore, the plant disease caused by the anthracnose bacteria is cucumber anthracnose [Colletotrichumorbiculare].

[0040] Among them, cucumber anthracnose can occur in the seedling and adult stages of cucumbers, which will affect the cucumber plants and their yields, and thus cause economic losses to growers.

[0041] The present invention has the following beneficial effects:

[0042] 1) The bactericidal composition provided by the present invention has high bactericidal activity and has a synergistic effect on the target at a certain mass ratio;

[0043] 2) The fungicidal composition provided by the present invention can significantly improve the quality and quality of crops;

[0044] 3) The fungicide composition provided by the present invention is safe and efficient, and is safe to crops, non-target organisms, beneficial organisms and natural enemies. DETAILED DESCRIPTION

[0045] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0046] In-house toxicity assay:

[0047] Indoor bioassay of cucumber anthracnose

[0048] Test basis: NY / T 1156.2-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 2: Plate Method for Inhibition of Pathogenic Fungal Mycelial Growth"; NY / T 1156.6-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 6 Determination of Combined Action of Mixtures".

[0049] Test pathogen: Cucumber anthracnose (isolated from anthracnose lesions showing typical symptoms on Xiazhuang cucumber fruits).

[0050] Test agents: 95% bromothionil technical, 95% prochloraz technical, 90% technical of the compound of formula (I), all of which are provided by the group's research and development center.

[0051] Preparation of drugs: Dissolve the test drug in acetone first, and then dilute it with 0.1% Tween 80 aqueous solution. Prepare single-dose stock solutions respectively, and set 5 series of mass concentrations according to the purpose of mixing and the activity of the drug.

[0052] After melting the PDA culture medium in a microwave oven, cool it to about 50°C. According to the principle of from low concentration to high concentration, take 1 mL of the prepared drug solution to be tested and 9 mL of PDA culture medium and add them to a culture dish with a diameter of 9 cm. Mix well to make a drug-containing plate of corresponding concentration.

[0053] Inoculation: Use a 6mm diameter puncher to punch the activated pathogenic fungi into a cake under sterile conditions. After the drug-containing culture medium solidifies, place the cake in the center of the culture medium. Finally, seal the culture dish with a sealing film and place it in an incubator at 27°C and a relative humidity of 70%. At the same time, set up a blank control without drug. Each treatment is repeated 3 times.

[0054] Investigation: Investigate the growth of pathogenic mycelium based on the growth of mycelium in the blank control culture dish, and measure the colony diameter with a caliper in millimeters (mm). Measure the diameter of each colony vertically once using the cross method and take the average value.

[0055] Calculation method: According to the survey results, the mycelium growth inhibition rate is calculated according to the following formula. The unit is percentage (%), and the calculation result is rounded to two decimal places.

[0056] D=D 1 -D 2

[0057] Where:

[0058] D——colony growth diameter;

[0059] D 1 ——colony diameter;

[0060] D 2 ——Diameter of mushroom cake.

[0061]

[0062] I——hyphae growth inhibition rate;

[0063] D 0 ——Blank control colony growth diameter;

[0064] D t ——The growth diameter of the colony after treatment with chemicals.

[0065] Test statistics: The data were processed by probability value analysis. The DPS statistical analysis system was used to analyze the toxicity regression line and EC 50 Value, to evaluate the activity of the test agent on the biological test material.

[0066] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of ≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; and 80<CTC<120 indicates an additive effect.

[0067] The co-toxicity coefficient (CTC value) of the mixture is calculated by the following formula:

[0068]

[0069] Where:

[0070] ATI——Actual Toxicity Index of Mixture;

[0071] S——EC of standard fungicide 50 , the unit is milligrams per liter (mg / L);

[0072] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0073] TTI=TI A *P A+TI B *P B

[0074] Where:

[0075] TTI – Theoretical Toxicity Index of Mixtures;

[0076] TI A ——A: toxicity index of the agent;

[0077] P A ——The percentage of agent A in the mixture, in percentage (%);

[0078] TI B ——Toxicity index of agent B;

[0079] P B ——The percentage of agent B in the mixture, in percentage (%).

[0080]

[0081] Where:

[0082] CTC – Co-toxicity coefficient;

[0083] ATI——Actual Toxicity Index of Mixture;

[0084] TTI - Theoretical Toxicity Index of Mixture.

[0085] Measurement results:

[0086] The results in Table 1 show that the compound of formula (I) can prevent and treat cucumber anthracnose EC 50 1.522mg / L, bromothionil for the control of cucumber anthracnose EC 50 The compound (I) and bromothionil have a mass ratio of 1:35 to 28:1, which shows an additive or synergistic effect. When the mass ratio is 1:28 to 28:1, the co-toxicity coefficient is greater than 120, showing a synergistic effect. The co-toxicity coefficient of the compound (I) and bromothionil = 2:5 is the largest, which is 282.599, EC 50 It is 0.121mg / L, and the synergistic effect is significant.

[0087] Table 1 The results of the synergistic effect of different ratios of the compound of formula (I) and bromothionil on cucumber anthracnose

[0088]

[0089] The results in Table 2 show that the compound of formula (I) can prevent and treat cucumber anthracnose EC 50 The EC value of prochloraz for the control of cucumber anthracnose is 1.516 mg / L. 50The compound (I) and prochloraz are mixed in a mass ratio of 1:32 to 34:1, showing an additive or synergistic effect. When the mass ratio is 1:32 to 26:1, the co-toxicity coefficient is greater than 120, showing a synergistic effect. The co-toxicity coefficient of the compound (I) and prochloraz = 2:3 is the largest, which is 272.477, EC 50 It is 1.131 mg / L, with significant synergistic effect.

[0090] Table 2 The results of the synergistic effect of different ratios of the compound of formula (I) and prochloraz on cucumber anthracnose

[0091]

[0092]

[0093] Formulation Examples

[0094] Preparation Example 1:

[0095] 35% formula (I) compound bromothionil wettable powder (10:25)

[0096] Preparation formula: 10% of the compound of formula (I), 25% of bromothionil, 5% of polycarboxylic acid sodium salt, 2% of α-olefin sulfonate, 5% of dispersant NNO, 6% of sodium lignin sulfonate, 5% of sodium alkyl polyoxyethylene ether sulfonate, 5% of attapulgite, and the balance is made up of kaolin;

[0097] Preparation method: Active ingredients, other functional adjuvants and fillers are mixed according to the formula ratio, stirred evenly in a stirring kettle, and ground and mixed evenly for multiple times by a jet mill to prepare the wettable powder of the composition of the present invention.

[0098] Preparation Example 2:

[0099] 50% compound of formula (I)·Prochloraz WP (18:32)

[0100] Preparation formula: 18% of the compound of formula (I), 32% of prochloraz, 7% of alkylphenol polyoxyethylene ether methyl ether condensate sulfate, 5% of dispersant NNO, 5% of sodium alkyl polyoxyethylene ether sulfonate, 3.5% of sodium dodecyl sulfate, 5.5% of bentonite, 1.5% of white carbon black, and kaolin to make up the balance;

[0101] Preparation method: Same as Preparation Example 1.

[0102] Preparation Example 3:

[0103] 28% compound of formula (I)·bromothionil water emulsion (8:20)

[0104] Formula: 8% of compound of formula (I), 20% of bromothionil, 12% of trimethylbenzene, 15% of cyclohexanone, 1% of calcium dodecylbenzenesulfonate, 0.5% of sodium sulfate of fatty alcohol polyoxyethylene ether, 5% of EO / PO block copolymer, 5% of glycerol, 1.5% of glycerol, 0.1% of silicone oil, 0.2% of xanthan gum, 0.1% of sodium benzoate, and the balance is made up with deionized water;

[0105] Preparation method: according to the formula ratio of the embodiment, the active ingredient is dissolved in the solvent and an emulsifier is added to dissolve it into a uniform oil phase, and deionized water and antifreeze are mixed together to form a uniform water phase; under high-speed shearing, the water phase is added to the oil phase to form a well-dispersed water emulsion preparation.

[0106] Preparation Example 4:

[0107] 25% compound of formula (I)·prochloraz aqueous emulsion (12:13)

[0108] Formula: 12% of compound of formula (I), 13% of prochloraz, 11.5% of trimethylol, 10% of cyclohexanone, 1% of calcium dodecylbenzenesulfonate, 0.5% of sodium sulfate of fatty alcohol polyoxyethylene ether, 5% of EO / PO block copolymer, 5% of ethylene glycol, 1.2% of glycerol, 0.1% of silicone oil, 0.2% of xanthan gum, 0.1% of sodium benzoate, and the balance is made up with deionized water;

[0109] Preparation method: Same as Preparation Example 3.

[0110] Preparation Example 5:

[0111] 20% formula (I) compound · bromothionil emulsifiable concentrate (8:12)

[0112] Preparation formula: 8% of the compound of formula (I), 12% of bromothionil, 1% of BHT, 10% of propylene carbonate, 15% of DMF, 4.5% of cyclohexanone, 4% of Nongru 700#, 3.5% of calcium dodecylbenzene sulfonate, 1% of alkylphenol polyoxyethylene ether, 10% of glycerol fatty acid ester polyoxyethylene ether, and methyl oleate to make up the balance;

[0113] Preparation method: Add the active ingredients into the co-solvent according to the formula ratio of the embodiment, and add the surfactant and other functional additives thereto, stir and mix them evenly in a stirring mixing kettle to obtain the emulsifiable concentrate.

[0114] Preparation Example 6:

[0115] 30% compound of formula (I)·Prochloraz EC (12:18)

[0116] Preparation formula: 12% of the compound of formula (I), 18% of prochloraz, 1.5% of BHT, 10% of propylene carbonate, 15% of DMF, 5% of cyclohexanone, 3.5% of calcium dodecylbenzenesulfonate, 10% of glycerol fatty acid ester polyoxyethylene ether, and the balance is made up of methyl oleate;

[0117] Preparation method: Same as Preparation Example 5.

[0118] Field efficacy test

[0119] Field test on the efficacy of pesticides against cucumber anthracnose

[0120] Experimental location: Wangjiayu vegetable greenhouse in Feicheng City, Tai'an City, Shandong Province;

[0121] Test crop: cucumber (Jinza No. 1); water and fertilizer conditions and growth status are consistent, and it is more susceptible to disease. The conditions meet the requirements of field trials.

[0122] Test time: Planting on December 5, 2023, cultivated in protected area, the test was in the fruit-bearing period, and the growth was excellent.

[0123] Test drug and dosage:

[0124] Table 4 Comparison table of dosage of field test

[0125]

[0126]

[0127] Experimental treatment: single-dose and clean water control were set, 7 days between two applications, and each treatment was repeated 4 times. The area of ​​the plot was divided into 20m 2 , randomized block arrangement. Avoid rain, strong wind and extreme high temperature during application. The application equipment is 3WBD-18L backpack electric sprayer, and the spray liquid volume is 675L / hm 2 .

[0128] Survey time and frequency: Disease surveys were conducted before the first application of pesticides, 7 days after the first application of pesticides, and 7 days after the last application of pesticides, for a total of 3 surveys, and the number of diseased leaves and relative grade values ​​were counted.

[0129] Survey method: Select 5 points along the diagonal line in each plot, mark 3 plants with signs at no points, check 10 leaves from bottom to top for each plant, and check a total of 15 plants and 150 leaves.

[0130] The classification method refers to "GB / T 17980.112-2004 Pesticide Field Efficacy Test Guidelines (II) Fungicides for Control of Anthracnose in Cucurbits", and the specific classification is as follows:

[0131] There are 9 levels in total:

[0132] Level 0: no lesions;

[0133] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0134] Level 3: The lesion area accounts for 6% to 10% of the entire leaf area;

[0135] Level 5: The lesion area accounts for 11% to 25% of the entire leaf area;

[0136] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0137] Level 9: The lesion area accounts for more than 51% of the entire leaf area;

[0138] Calculation method of disease index and drug efficacy:

[0139]

[0140] During the experiment, cucumbers in each treatment plot grew well, and no treatment agent caused phytotoxicity to cucumber plants and other non-target organisms at the tested concentrations. The experimental data were analyzed by Duncan's new multiple range method (DMRT) biostatistics using DPS software, and the significance of the differences was evaluated.

[0141] Field efficacy test results:

[0142] The results showed that 35% of the compound of formula (I)·bromothionil wettable powder (10:25) and 25% of the compound of formula (I)·myclobutanil emulsion (12:13) had good control effects on cucumber anthracnose. Seven days after the first application, the control effects were 76.35% and 74.29%, respectively; there was a significant difference between the mixed preparations and other single agents.

[0143] Table 5 Field efficacy test results (7 days after the first application)

[0144]

[0145] Note: The data in the table are the average values ​​of 4 replicates; lowercase letters indicate significant differences among treatments (p < 0.05)

[0146] The results showed that 35% of the compound of formula (I)·bromothionil wettable powder (10:25) and 25% of the compound of formula (I)·myclobutanil emulsion (12:13) had good control effects on cucumber anthracnose. Seven days after the last application, the control effects were 83.33% and 82.57%, respectively; there was a significant difference between the mixed preparations and other single agents.

[0147] Table 6 Field efficacy test results (7 days after the last application)

[0148]

[0149] Note: The data in the table are the average values ​​of 4 replicates; lowercase letters indicate significant differences among treatments (p < 0.05)

[0150] During the test, no pesticide damage or impact on crop growth and development was recorded, indicating that the test pesticide is safe for crops within the recommended dosage. No effects of the pesticide on non-target organisms within the dosage range were found. No extreme weather conditions such as heavy rain and strong wind were recorded during the test.

[0151] The results of the field efficacy plot test showed that the compound represented by formula (I) and bromothionil and prochloraz showed a high control effect in the control of cucumber anthracnose, which not only reduced the diseased fruit rate and disease index, but also had a significant control effect, and had no significant impact on the yield. The mixed preparation also showed a significant yield-increasing effect. In addition, according to the observation after the drug application, the cucumbers in each treatment area grew normally, and no drug damage occurred, indicating that the cucumbers grew safely at the dosage of each agent in this test.

[0152] Through indoor toxicity determination and field tests, the composition of the compound represented by the formula (I) of the present invention and any one of bromothionil and prochloraz showed very good control effect on cucumber anthracnose. The fungicidal composition or its preparation obtained by compounding of the present invention has significant control effect, and has the characteristics of high efficiency, broad spectrum, low residue, long lasting effect, strong systemicity, etc. In addition, no phytotoxicity of the compounded agent to crops was found in the test, indicating that when the fungicidal synergistic effect of the obtained fungicidal composition or preparation is improved, the production cost and use cost can be reduced, and it is safe for crops.

[0153] Although the present invention has been described in detail above with general instructions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A bactericidal composition, characterized in that: It contains active ingredient A and active ingredient B, wherein the active ingredient A is a compound represented by formula (I), and the chemical structure is: The active ingredient B is any one of bromothionil and prochloraz.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:35 to 34:1; Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 28:

1.

3. The bactericidal composition according to claim 2, characterized in that The mass ratio of the compound of formula (I) to bromothionil is 1:28 to 28:1, and the mass ratio of the compound of formula (I) to prochloraz is 1:32 to 26:1; Preferably, the mass ratio of the compound of formula (I) to bromothionil is 1:28 to 14:1, and the mass ratio of the compound of formula (I) to prochloraz is 1:32 to 13:

1.

4. The bactericidal composition according to claim 1, characterized in that Based on the total weight of the bactericidal composition being 100 wt %, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 0.5 to 90 wt %; Preferably, the total content of the active ingredient A and the active ingredient B in the bactericidal composition is 1 to 80 wt %.

5. The bactericidal composition according to claim 1, characterized in that In addition to the active ingredients, the fungicide composition also contains agriculturally permitted auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers.

6. The bactericidal composition according to claim 5, characterized in that The dosage form of the bactericidal composition is selected from solid preparations and / or liquid preparations.

7. The bactericidal composition according to claim 6, characterized in that The fungicide composition can be prepared into a pesticide-acceptable formulation; Preferably, the formulation is in the form of a microemulsion, emulsifiable concentrate, suspension, aqueous emulsion, water dispersible granules, wettable powder, or seed treatment suspension.

8. Use of the fungicidal composition according to any one of claims 1 to 7 in preventing and controlling plant diseases.

9. The use according to claim 8, characterized in that: The plant diseases are plant diseases caused by fungi or bacteria.

10. The use according to claim 9, characterized in that: The plant disease is a plant disease caused by Anthracnose fungi; Preferably, the plant disease caused by the Anthracnose fungus is cucumber anthracnose.

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