Air staged injection of fluids in flow cells for SPR analysis

By sequentially introducing multiple sample solutions on the sensor surface and separating the samples using gas sections, combined with the cyclic subtraction method, the interference problem of multiple injection analysis in the prior art is solved, and more accurate sample behavior analysis is achieved.

CN119968559APending Publication Date: 2025-05-09CYTIVA SWEDEN AB
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Patent Information

Application Number
CN202380061624.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2022-09-21
Filing Date
2023-08-16
Publication Date
2025-05-09

AI Technical Summary

Technical Problem

Existing systems and methods are difficult to perform multi-injection analysis efficiently, especially when using running buffers between samples, resulting in the analyte being washed away, dissociated or disturbed, and it is difficult to accurately analyze sample behavior.

Method used

Sample mixing is avoided until the samples reach the sensor surface without interacting until the samples reach the sensor surface. At the same time, the cyclic subtraction method is used to replace the sample by buffer samples, and the binding signal is subtracted to remove unwanted signal interference.

Benefits of technology

The sequential injection of samples without intermediate running buffer is achieved, avoiding interference between samples, and more accurately analyzing the interactions and behaviors between samples, improving the effectiveness and accuracy of the analysis.

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Abstract

According to various aspects, the present invention provides a method of delivering a plurality of sample solutions to a sensor surface (102) for analysis. It may additionally or alternatively provide systems and methods for enabling an improved study of analyte behavior. A sensor (102) may be used, for example, to determine kinetic binding properties of an analyte, such as a protein, to a surface binding ligand. In one aspect, a method includes: a) introducing (902) a first sample solution (502) into a first channel (506), the first channel (506) configured to deliver the sample solution to a sensor surface (102); b) introducing (904) a second sample solution (504) into the first channel (506); c) flowing (906) the first sample solution and the second sample solution through the first channel (506) and over the sensor surface (102); and d) detecting (912) at the sensor surface (102) the presence or absence of binding of the analyte from at least one of the sample solutions (502, 504) wherein no running buffer solution passes over the sensor surface (102) throughout steps a)-d).
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