Method for preventing and treating pepper bacterial wilt

Abstract

The application discloses a kind of capsicum blight prevention and treatment methods, belong to the field of biotechnology, include the following steps: when transplanting capsicum seedling and when first capsicum blight plant appears, compound microbial inoculant is used to the root irrigation of capsicum plant;The compound microbial inoculant includes the volume ratio of 3-5:1-2:1 of paecilomyces lilacinus bacteria suspension, mojavensis bacillus bacteria suspension and penicillium expansum bacteria suspension, wherein, the effective viable count of paecilomyces lilacinus bacteria suspension is: 2x10 8 CFU / mL, the effective viable count of mojavensis bacillus bacteria suspension and penicillium expansum bacteria suspension is: 1x10 8 CFU / mL.The prevention and treatment method disclosed by the application can effectively prevent and treat capsicum blight.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of vegetable disease prevention and treatment, in particular to a method for preventing and treating pepper blight. BACKGROUND

[0002] Pepper (Capsicum annum L.) is one of the largest vegetable crops in China. With the increase of the area of facility vegetable planting in China year by year, pepper blight has become a devastating soil-borne disease that seriously affects the yield and quality of pepper. The pathogen of pepper blight, Phyophthora capsici, has a wide host range and can survive in the soil for a long time. Once it is introduced, it is difficult to eradicate, and can cause the destruction of the greenhouse and cause huge economic losses, seriously threatening the sustainable development of the pepper industry in China.

[0003] The use of pesticides to prevent and treat pepper blight not only destroys the environment but also causes pesticide residue problems. At present, biological control is the most environmentally friendly control method. SUMMARY

[0004] In order to overcome the problems in the prior art, the present application provides a soft capsule of astaxanthin and a preparation process thereof. The present application optimizes the formulation of the auxiliary materials and the capsule wall to improve the stability and bioavailability of the astaxanthin in the capsule.

[0005] To achieve the above-mentioned purpose, the present application provides a method for preventing and treating pepper blight, comprising the following steps:

[0006] The compound microbial agent is used to irrigate the roots of the pepper plants when the pepper seedlings are transplanted and when the first pepper blight plant appears. The compound microbial agent comprises a Paecilomyces lilacinus suspension, a Bacillus mojavensis suspension and a Penicillium expansum suspension in a volume ratio of 3-5:1-2:1. The effective viable count of the Paecilomyces lilacinus suspension is 2×10 8 CFU / mL, and the effective viable count of the Bacillus mojavensis suspension and the Penicillium expansum suspension is 1×10 8 CFU / mL.

[0007] Further, when irrigating the roots of the pepper, the use amount of the compound microbial agent is 200-400 mL / plant.

[0008] Further, when the first pepper blight plant appears, the roots are irrigated continuously for 2-3 times with an interval of 7 days.

[0009] Through the above technical solution, the present application can effectively prevent and treat pepper blight. DETAILED DESCRIPTION

[0010] Unless otherwise specified, the materials and reagents used in the present application are commercially available.

[0011] In the present application: Paecilomyces lilacinus, Bacillus mojavensis and Penicillium expansum are purchased from Industrial Microbial Strain Engineering Technology Research Center; wherein the number of Paecilomyces lilacinus is: BNCC342068, the number of Penicillium expansum is: BNCC146144, and the number of Bacillus mojavensis is: BNCC358077.

[0012] The preparation method of the Paecilomyces lilacinus and Penicillium expansum suspension in the present application is: inoculating Paecilomyces lilacinus or Penicillium expansum in a liquid culture medium (sodium nitrate: 3.0 g, potassium phosphate: 1.0 g, magnesium sulfate: 0.5 g, potassium chloride: 0.5 g, ferrous sulfate: 0.01 g, sucrose: 30.0 g, water 1000 ml) for fermentation culture, 25-28℃, aerobic, fermentation culture for 2-3 days, to obtain Paecilomyces lilacinus or Penicillium expansum suspension.

[0013] The preparation method of the Bacillus mojavensis suspension in the present application is: inoculating the purchased Bacillus mojavensis test tube strain in NB liquid culture medium for culture, and culturing on a shaking bed at a temperature of 25-28℃ and a rotation speed of 200-220 r / min for 30-35 h to obtain liquid strain.

[0014] The liquid strain of Bacillus mojavensis is inoculated in a liquid fermentation medium (potato 95 g, corn starch 80 g, peptone 5 g, ammonium chloride 12 g, sucrose 10 g, water 1000 ml; pH = 7.2-7.5) at a volume ratio of 0.1:1 for fermentation culture, and the fermentation culture conditions are: 26-28℃, dissolved oxygen 60%-70%, stirring 180-200 r / min, and culturing for 36-42 h to obtain Bacillus mojavensis suspension.

[0015] Example 1

[0016] The complex microbial agent is used for root irrigation of the pepper plants when the pepper seedlings are transplanted and when the first pepper phytophthora parasitica strain appears; the complex microbial agent comprises Paecilomyces lilacinus suspension, Bacillus mojavensis suspension and Penicillium expansum suspension at a volume ratio of 4:1.5:1, wherein the effective viable count of the Paecilomyces lilacinus suspension is 2×10 8 CFU / mL, and the effective viable count of the Bacillus mojavensis suspension and the Penicillium expansum suspension is 1×10 8 CFU / mL.

[0017] Among them, the use amount of the complex microbial agent for root irrigation of the pepper is 300 mL / plant.

[0018] Among them, the root irrigation is continuously performed for 3 times with an interval of 7 days when the first pepper phytophthora parasitica strain appears.

[0019] Example 2

[0020] The pepper seedlings are transplanted and the first pepper phytophthora infestans appears, and then the complex microbial agent is used to irrigate the roots of the pepper plants; the complex microbial agent comprises a Paecilomyces lilacinus suspension, a Bacillus mojavensis suspension and a Penicillium expansum suspension in a volume ratio of 3:1:1, wherein the effective viable count of the Paecilomyces lilacinus suspension is 2x10 8 CFU / mL, and the effective viable count of the Bacillus mojavensis suspension and the Penicillium expansum suspension is 1x10 8 CFU / mL.

[0021] When the pepper plants are irrigated, the use amount of the complex microbial agent is 200 mL / plant.

[0022] The first pepper phytophthora infestans appears, and then the complex microbial agent is used to irrigate the roots of the pepper plants for two times with an interval of 7 days.

[0023] Example Three

[0024] The pepper seedlings are transplanted and the first pepper phytophthora infestans appears, and then the complex microbial agent is used to irrigate the roots of the pepper plants; the complex microbial agent comprises a Paecilomyces lilacinus suspension, a Bacillus mojavensis suspension and a Penicillium expansum suspension in a volume ratio of 5:2:1, wherein the effective viable count of the Paecilomyces lilacinus suspension is 2x10 8 CFU / mL, and the effective viable count of the Bacillus mojavensis suspension and the Penicillium expansum suspension is 1x10 8 CFU / mL.

[0025] When the pepper plants are irrigated, the use amount of the complex microbial agent is 400 mL / plant.

[0026] The first pepper phytophthora infestans appears, and then the complex microbial agent is used to irrigate the roots of the pepper plants for three times with an interval of 7 days.

[0027] Comparative Example One

[0028] Comparative Example One and Example One are different in that the complex microbial agent does not contain the Paecilomyces lilacinus suspension.

[0029] Comparative Example Two

[0030] Comparative Example Two and Example One are different in that the complex microbial agent does not contain the Bacillus mojavensis suspension.

[0031] Comparative Example Three

[0032] Comparative Example Three and Example One are different in that the complex microbial agent does not contain the Penicillium expansum suspension.

[0033] Pot Experiment

[0034] The pepper seedlings with consistent growth and no obvious diseases and pests are transplanted in pots, and the transplanted pepper seedlings are irrigated with 300 mL of the complex microbial inoculum per plant according to the complex microbial inoculum described in Examples 1 to 3 and Comparative Examples 1 to 3; and a blank control group without irrigation of the complex microbial inoculum is set. After 7 days of irrigation of the complex microbial inoculum, the pepper seedlings in the experimental group and the blank control group are irrigated with 200 mL of a pathogenic fungus Phyophthora capsici suspension (effective viable bacteria number 1×10 8 CFU / mL) per plant, and the incidence of Phytophthora capsici of the pepper seedlings is counted 15 days after the irrigation of the pathogenic fungus, and the incidence rate is calculated, and the counting results are shown in Table 1.

[0035] Incidence rate = (number of diseased plants / total number of transplanted plants) * 100%;

[0036] Table 1: Incidence of pepper seedlings in the potting test

[0037]

[0038]

[0039] As can be seen from Table 1, the prevention and treatment method described in the application can effectively prevent and treat pepper blight.

[0040] Finally, it should be pointed out that the above preferred embodiments are only used to illustrate the technical solutions of the application and are not limiting. Although the application has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined in the claims of the application.

Claims

1. A method for controlling phytophthora capsici, characterized by: The method comprises the following steps: using a complex microbial agent to irrigate the roots of the pepper plants when the pepper seedlings are transplanted and when the first pepper phytophthora infestans appears; the complex microbial agent comprises a Paecilomyces lilacinus suspension, a Bacillus mojavensis suspension and a Penicillium expansum suspension in a volume ratio of 3-5:1-2:1; the effective viable count of the Paecilomyces lilacinus suspension is 2×10 8 CFU / mL, and the effective viable count of the Bacillus mojavensis suspension and the Penicillium expansum suspension is 1×10 8 CFU / mL; wherein the Paecilomyces lilacinus, the Bacillus mojavensis and the Penicillium expansum are purchased from the Industrial Microbial Strain Engineering Technology Research Center; the number of the Paecilomyces lilacinus is BNCC342068, the number of the Penicillium expansum is BNCC146144, and the number of the Bacillus mojavensis is BNCC358077.

2. The method for preventing and treating pepper bacterial wilt according to claim 1, characterized in that: The amount of the complex microbial agent used for the pepper is 200-400 mL per plant.

3. The method for preventing and treating pepper bacterial wilt according to claim 1, characterized in that: The first strain of pepper phytophthora is continuously irrigated for 2-3 times with an interval of 7 days.

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