Composition for promoting skin microcirculation, makeup removing cream and preparation method of makeup removing cream

Abstract

The invention provides a composition for promoting skin microcirculation, makeup removing cream and a preparation method of the makeup removing cream, and relates to the technical field of skin care products. The composition for promoting skin microcirculation comprises an ingredient for inducing vasodilation, an ingredient for improving vascular tolerance, an ingredient for reducing free heme and an ingredient for relieving perceptual pressure, and skin care is realized on the basis of improving forward emotion perception and accelerating blood circulation. Compared with a traditional single microcirculation path, for example, human epidermal keratinocyte and normal vascular endothelial cells are purely promoted to synthesize nitric oxide, a nitric oxide mediated negative feedback loop is activated to stimulate smooth muscle mediated vasodilation, and blood circulation is not obviously promoted. According to the application, the preferable raw material design comprises the steps of inducing vasodilation, preventing a large amount of hemoglobin from permeating out of the skin and then degrading permeated free heme to realize a multi-channel closed loop to promote blood microcirculation, and active matters capable of regulating endokephin secretion are matched to further relax facial muscles and relieve the pressure skin.

Description

Technical Field

[0001] This application relates to the technical field of skin care products, and particularly to a composition for promoting skin microcirculation, a cleansing balm and a preparation method thereof. Background Art

[0002] With the improvement of skin care concepts, the importance of makeup removal is forming a general consensus. In traditional cognition, makeup removal is a part of daily cleaning. When first used, consumers' demands were more reflected in the cleaning power, such as deeply cleaning facial dirt and being able to easily wash off full-face makeup.

[0003] At the same time, makeup removal is an important bridge connecting cleaning and skin care. As the first step in starting skin care, under the trend of multi-functional products, makeup removal products do not need to be only defined as cleaning products. The concept of "skin-nourishing makeup removal" has begun to be popular in the current market. Compared with makeup remover lotions, new makeup removal products such as makeup remover oils and cleansing balms, as oil-based products, not only have better makeup dissolving ability due to the principle of similar compatibility, but also supplement oil to the skin, strengthen the sebum film, and achieve a better effect of nourishing the skin with oil, and thus are more favored by consumers.

[0004] Although the concept of skin care already exists in current makeup removal products, the so-called "skin nourishing" mainly reflects in aspects such as improving the mildness of the product, using a large amount of oil to reduce friction on the skin, and selecting natural oils. Essentially, it still revolves around the cleaning property of the cleansing balm, and the skin nourishing is mostly a conceptual claim without substantial effect. Summary of the Invention

[0005] The purpose of this application is to provide a composition for promoting skin microcirculation, a cleansing balm and a preparation method thereof, aiming to solve the problem that existing makeup removal products have no substantial skin care effect.

[0006] To achieve the above purpose, this application provides a composition for promoting skin microcirculation, which includes, by mass percentage: 0.1%-1% of vasodilatory induction components, 0.1%-1% of components for improving vascular tolerance, 0.06%-0.6% of components for reducing free heme, and 0.01%-0.3% of components for relieving perceived stress.

[0007] In some embodiments, the vasodilatory induction components include any one of safflower extract, camellia seed oil, bitter orange oil, ginkgo leaf extract, and green tea extract;

[0008] Preferably, the vasodilatory induction components include camellia seed oil.

[0009] In some embodiments, the components for improving vascular tolerance include any one of citrus fruit extract, horse chestnut extract, centella asiatica extract, bilberry extract, and sophora japonica flower extract;

[0010] Preferably, the component for improving vascular tolerance includes: citrus fruit extract.

[0011] In some embodiments, the component for reducing free heme includes any one of: fucus extract, crataegus monogyna flower extract, and gardenia fruit bud extract;

[0012] Preferably, the component for reducing free heme includes: fucus extract.

[0013] In some embodiments, the component for relieving perceived stress includes any one of: jasminum grandiflorum extract, biogum - 2, saffron extract, melissa officinalis extract, and tephrosia purpurea seed extract;

[0014] Preferably, the component for relieving perceived stress includes: jasminum grandiflorum extract.

[0015] In some embodiments, by mass percentage, it includes: 0.1% - 1% of camellia seed oil, 0.1% - 1% of citrus fruit extract, 0.06% - 0.6% of fucus extract, and 0.01% - 0.3% of jasminum grandiflorum extract;

[0016] Optionally, by mass percentage, it includes: 0.5% - 1% of camellia seed oil, 0.5% - 1% of citrus fruit extract, 0.3% - 0.6% of fucus extract, and 0.05% - 0.3% of jasminum grandiflorum extract.

[0017] This application also provides a cleansing balm, which includes the composition for promoting skin microcirculation described above.

[0018] In some embodiments, it further includes: 50% - 90% of emollient, 5% - 25% of non - ionic emulsifier, 4% - 8% of oil - phase thickener, and 0.1 - 0.5% of preservative.

[0019] This application also provides a preparation method of the above - mentioned cleansing balm, including:

[0020] Adding the emollient, non - ionic emulsifier, and oil - phase thickener into a container, stirring and heating until the materials are completely melted, cooling down, and sequentially adding the composition for promoting skin microcirculation and the preservative.

[0021] In some embodiments, the stirring speed is 300 - 500 rpm, the heating temperature is up to 85 ± 5 °C, and the temperature is cooled down to 55 - 60 °C.

[0022] Compared with the prior art, the beneficial effects of this application include:

[0023] The composition for promoting skin microcirculation provided by the present application includes components for inducing vasodilation, improving vascular tolerance, reducing free heme, and relieving perceived stress. It achieves skin maintenance based on improving positive emotion perception and accelerating blood circulation. Compared with traditional and relatively single microcirculation paths, such as simply promoting the synthesis of nitric oxide by human epidermal keratinocytes and normal vascular endothelial cells, activating the nitric oxide-mediated negative feedback loop, and then stimulating smooth muscle-mediated vasodilation, the promotion of blood circulation is not obvious. The preferred raw material design of the solution of the present application realizes multi-path closed-loop promotion of blood microcirculation from inducing vasodilation to avoiding massive penetration of hemoglobin outside the skin and then degrading the penetrated free heme, and further relaxes facial muscles and soothes stressed skin by combining active substances that can regulate the secretion of endorphins.

[0024] Compared with promoting metabolism solely by promoting blood flow in the traditional way, the composition of the present application has a complete blood microcirculation promotion link. The preferred active ingredients form a complete closed loop from inducing vasodilation to promoting blood flow, improving vascular tolerance to reduce hemoglobin leakage, and then specifically degrading the leaked hemoglobin.

[0025] Based on generalized skin microcirculation, the present application three-dimensionally enhances skin microcirculation in terms of perception regulation and blood regulation. While promoting blood microcirculation, it helps relax facial muscles and release pleasure factors to relieve skin stress, giving consumers a better experience when using.

[0026] Combined with the formulation characteristics and usage scenarios of oil-based makeup remover products, the composition for promoting skin microcirculation is designed to be applied to oil-based makeup remover products, and it is verified by comparison that the oil-based makeup remover dosage form has a more superior positive promoting effect on skin microcirculation than other water-based makeup remover dosage forms or traditional skin care products. Description of the Drawings

[0027] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present application and should not be regarded as limiting the scope of the present application.

[0028] Figure 1 Statistical data of the β-endorphin release rate in cells after adding the compositions of the examples and the comparative examples;

[0029] Figure 2 Statistical data of the HO-1 concentration in cells after adding the compositions of the examples and the comparative examples;

[0030] Figure 3 Cell scratch test data of the examples and the comparative examples;

[0031] Figure 4 Cell scratch healing effect diagrams of the examples and the comparative examples;

[0032] Figure 5 It is a graph of the experimental results of nailfold microcirculation detection. Detailed implementation manners

[0033] As used herein, the terms:

[0034] "Prepared from" is synonymous with "comprising". As used herein, the terms "comprising", "including", "having", "containing" or any other variation thereof are intended to cover non-exclusive inclusion. For example, a composition, step, method, article or device comprising the listed elements is not necessarily limited to those elements, but may include other elements not expressly listed or elements inherent to such composition, step, method, article or device.

[0035] The connecting word "consisting of" excludes any unstated element, step or component. If used in a claim, this phrase will render the claim closed, excluding materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of" appears in a clause of the claim body rather than immediately following the subject, it only limits the elements described in that clause; other elements are not excluded from the claim as a whole.

[0036] When an equivalent, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper preferred values and lower preferred values, this should be understood to specifically disclose all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value, regardless of whether the ranges are separately disclosed. For example, when the range "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When a numerical range is described herein, unless otherwise stated, the range is intended to include its end values and all integers and fractions within the range.

[0037] In these examples, unless otherwise specified, the parts and percentages are by mass.

[0038] "Parts by mass" refers to the basic measurement unit representing the mass ratio relationship of multiple components. 1 part can represent any unit mass, such as 1 g or 2.689 g, etc. If we say that the mass part of component A is a parts and the mass part of component B is b parts, it means the mass ratio of component A to component B is a:b. Or, it means the mass of component A is aK and the mass of component B is bK (K is any number representing a multiple factor). It should not be misunderstood that, different from the mass fraction, the sum of the mass parts of all components is not limited to 100 parts.

[0039] "And / or" is used to indicate that one or both of the stated situations may occur. For example, A and / or B includes (A and B) and (A or B).

[0040] The present application provides a composition for promoting skin microcirculation, which, by mass percentage, includes: 0.1%-1% of an ingredient for inducing vasodilation, 0.1%-1% of an ingredient for improving vascular tolerance, 0.06%-0.6% of an ingredient for reducing free heme, and 0.01%-0.3% of an ingredient for relieving perceived stress.

[0041] The composition for promoting skin microcirculation provided by the present application includes an ingredient for inducing vasodilation, an ingredient for improving vascular tolerance, an ingredient for reducing free heme, and an ingredient for relieving perceived stress, and realizes skin care based on improving positive emotion perception and accelerating blood circulation. Compared with traditional and relatively single microcirculation paths, such as simply promoting nitric oxide synthesis by human epidermal keratinocytes and normal vascular endothelial cells, activating the nitric oxide-mediated negative feedback loop, and then stimulating smooth muscle-mediated vasodilation, the promotion of blood circulation is not obvious. The preferred raw material design of the present application scheme realizes multi-path closed-loop promotion of blood microcirculation from inducing vasodilation to avoiding a large amount of hemoglobin from penetrating outside the skin and then degrading the penetrated free heme, and further relaxes facial muscles and soothes stressed skin by matching with an active substance that can regulate endorphin secretion.

[0042] In some embodiments, the ingredient for inducing vasodilation includes any one of safflower extract, camellia seed oil, bitter orange oil, ginkgo leaf extract, and green tea extract;

[0043] Preferably, the ingredient for inducing vasodilation includes camellia seed oil. In the embodiment, the selected raw material is commercially named DF419 camellia seed oil BL-051, and the camellia seed oil content is 100%.

[0044] Camellia seed oil can promote blood circulation by regulating the content of high and low density lipoprotein cholesterol in the blood, reducing the degree of platelet aggregation, neutralizing hydrogen ions in the blood to reduce blood acidity, providing sufficient oxygen and nutrients for the skin, and timely removing the metabolic products of cells and various harmful substances such as superoxide anions.

[0045] In some embodiments, the ingredient for improving vascular tolerance includes any one of citrus fruit extract, horse chestnut extract, centella asiatica extract, bilberry extract, and sophora japonica flower extract;

[0046] Preferably, the ingredient for improving vascular tolerance includes citrus fruit extract. In the embodiment, the selected raw material is commercially named Calmnerv CR, and the citrus fruit extract content is 20%.

[0047] The citrus fruit extract can help fragile capillaries balance sudden vasodilation, avoid the lymphatic system from processing a large amount of leakage simultaneously in a short time, and reduce the infiltration of blood into the surrounding tissues.

[0048] In some embodiments, the component for reducing free heme includes any one of Fucus vesiculosus extract, Crataegus monogyna flower extract, and Gardenia jasminoides fruit bud extract;

[0049] Preferably, the component for reducing free heme includes Fucus vesiculosus extract, and the raw material selected in the embodiment has the trade name Shadownyl TM , and the content of Fucus vesiculosus extract is 4-7%.

[0050] The heme oxygenase-1 (HO-1) contained in the Fucus vesiculosus extract can catalyze the degradation of free heme produced by leaked hemoglobin into some metabolite with cytoprotective effects.

[0051] In some embodiments, the component for relieving perceived stress includes any one of Jasminum grandiflorum extract, Biosaccharide Gum-2, Crocus sativus extract, Melissa officinalis extract, and Tephrosia purpurea seed extract;

[0052] Preferably, the component for relieving perceived stress includes Jasminum grandiflorum extract, and the raw material selected in the embodiment has the trade name caressense TM biofunctional, and the content of Jasminum grandiflorum extract is 20%.

[0053] The Jasminum grandiflorum extract activates the skin tactile sensors, relieves perceived stress, promotes the release of the pleasure molecule β-endorphin that reduces pain, and increases the sense of comfort and pleasure.

[0054] In some embodiments, by mass percentage, it includes: 0.1%-1% of camellia seed oil, 0.1%-1% of citrus fruit extract, 0.06%-0.6% of Fucus vesiculosus extract, and 0.01%-0.3% of Jasminum grandiflorum extract.

[0055] Among them, the camellia seed oil can be, for example, 0.1%, 0.3%, 0.5%, 1% or any value between 0.1%-1%; the citrus fruit extract can be, for example, 0.1%, 0.3%, 0.5%, 1% or any value between 0.1%-1%; the Fucus vesiculosus extract can be, for example, 0.06%, 0.1%, 0.3%, 0.6% or any value between 0.06%-0.6%; the Jasminum grandiflorum extract can be, for example, 0.01%, 0.03%, 0.05%, 0.1%, 0.3% or any value between 0.01%-0.3%.

[0056] Optionally, by mass percentage, it includes: 0.5%-1% of camellia seed oil, 0.5%-1% of citrus fruit extract, 0.3%-0.6% of fucus vesiculosus extract, and 0.05%-0.3% of jasminum grandiflorum extract.

[0057] This application also provides a cleansing balm, which includes the above composition for promoting skin microcirculation.

[0058] The composition for promoting skin microcirculation of this application is applied in an oil-based cleansing product. The high oil content of the oil-based cleansing product makes it smoother and unobstructed during use, avoiding secondary skin damage caused by skin tugging. At the same time, combined with the appropriate rubbing and massaging in the use scenario of the cleansing balm, the effect of skin microcirculation can be further amplified in multiple aspects.

[0059] Specifically, in terms of perception regulation, massage can increase parasympathetic nerve signals and vagus nerve outflow to inhibit the stress response, thereby reducing anxiety and making the individual relax; in terms of blood regulation, massage enhances the shear stress of the blood vessel wall, dilates the blood vessels, and promotes an increase in skin blood flow; in terms of muscle regulation, high-pressure massage is not the most effective. Cleansing is a kind of massage under the self-regulated perception of "comfortable" pressure by consumers, which can reduce the persistent cross-bridges between myosin heads and actin and regulate muscle stiffness.

[0060] In some embodiments, it further includes: 50%-90% of emollient, 5%-25% of non-ionic emulsifier, 4%-8% of oil-phase thickener, and 0.1-0.5% of preservative.

[0061] In some embodiments, by mass percentage, the cleansing balm of this application includes:

[0062] The first emollient, cetearyl octanoate / caprate, has a weight percentage of 22-58%;

[0063] The second emollient, triglyceride (ethylhexanoic acid), has a weight percentage of 10-40%;

[0064] The third emollient, caprylic / capric triglyceride, has a weight percentage of 2-8%;

[0065] The first emulsifier, PEG-20 glyceryl triisostearate, has a weight percentage of 11-16%;

[0066] The second emulsifier, PEG-12 diisostearate, has a weight percentage of 2-8%;

[0067] The third emulsifier, PEG-40 sorbitan tetraoleate, has a weight percentage of 1-4%;

[0068] The fourth emulsifier, PEG-3 glyceryl isostearate, has a weight percentage of 1-3%;

[0069] The weight percentage of the first oil-phase thickener silica is 4-6%;

[0070] The weight percentage of the second oil-phase thickener synthetic wax is 1-2%;

[0071] The weight percentage of the preservative phenoxyethanol is 0.2-0.5%;

[0072] And the above composition for promoting microcirculation of the skin.

[0073] This application also provides a preparation method of the above makeup remover paste, including:

[0074] Add the emollient, nonionic emulsifier and oil-phase thickener into a container, stir and heat until the materials are completely melted, then cool down, and sequentially add the composition for promoting microcirculation of the skin and the preservative.

[0075] In some embodiments, the stirring speed is 300-500 rpm, for example, it can be 300 rpm, 350 rpm, 400 rpm, 450 rpm, 500 rpm or any value between 300-500 rpm, the heating temperature is up to 85±5°C, and the temperature is cooled down to 55-60°C.

[0076] The following will describe the implementation scheme of this application in detail with specific embodiments. However, those skilled in the art will understand that the following embodiments are only used to illustrate this application and should not be regarded as limiting the scope of this application. For those not specified in the embodiments, they are carried out according to the conventional conditions or the conditions recommended by the manufacturer. For the reagents or instruments not specified by the manufacturer, they are all conventional products that can be obtained through commercial purchase.

[0077] Example 1

[0078] Example 1 provides a composition for promoting microcirculation of the skin. By mass percentage, it includes: 0.5% camellia seed oil, 0.5% citrus fruit extract, 0.3% fucus extract and 0.05% jasmine flower extract, and it is added with water to 100% during the efficacy test.

[0079] The preparation method of the composition for promoting microcirculation of the skin in Example 1 includes:

[0080] Weigh and mix camellia seed oil, citrus fruit extract, fucus extract, and jasmine flower extract according to the required weight percentages, slowly add pure water to make it even, and the solution is an oil-water mixture. During the efficacy test, the composition needs to be mixed evenly by high-speed shaking with a vortex mixer for 5 minutes. When the product is applied, it can be directly added to the product according to the required weight ratio.

[0081] According to the formula in Table 1 and referring to the method of Example 1, the compositions of other Examples 2 to 12 and Comparative Examples 1 to 4 were respectively prepared, and their effects were tested.

[0082] Formulation of the compositions of each example and comparative example in Table 1

[0083]

[0084] Application example

[0085] The composition for promoting skin microcirculation of Example 1 was respectively applied to cleansing balm (Example A), aqueous cleansing lotion (Comparative Example A), and lotion (Comparative Example B) according to the formula shown in Table 2.

[0086] The preparation method includes the following steps:

[0087] Put the components other than the composition for promoting skin microcirculation into the pot in proportion, stir at a speed of 400 rpm, heat to 85 ± 5 °C, and stir and heat until the materials are completely melted; after stirring evenly, cool down to 55 - 60 °C; sequentially add the composition for promoting skin microcirculation and other active ingredients under low-speed stirring.

[0088] Formulation of each application product in Table 2

[0089]

[0090]

[0091] Efficacy evaluation

[0092] 1. Evaluation of the pressure relief of the composition: β-endorphin content determination experiment

[0093] 1.1 Test principle

[0094] Endorphin is a polypeptide neurotransmitter among many members of opioid peptides. It is released when the body feels pain or stress, helps to relieve pain and stress, and is also released during some exercises to enhance people's sense of happiness through the body's reward signaling pathway. The effect of the sample on stress relief can be evaluated by detecting the change in the concentration of human β-endorphin in the sample. The level of human β-endorphin (β-EP) in the sample is determined by competitive enzyme-linked immunosorbent assay (ELISA). To the enzyme-labeled wells pre-coated with human β-endorphin antigen, add the standard product and the sample. After incubation, add the biotin-labeled anti-β-endorphin antibody. Then combine with the HRP-labeled detection antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, add the substrate TMB for color development. TMB is converted into blue under the catalysis of HRP enzyme and into the final yellow under the action of acid. The depth of the color is positively correlated with the human β-endorphin in the sample.

[0095] 1.2 Test method

[0096] Adjust the density of the human immortalized keratinocyte HaCat suspension to 30,000 cells / well and inoculate it into a 96-well plate, 100 μL / well, and place it in an incubator at 37 °C with 5% CO 2 Cultivate for 24 h. Set up a blank control group and a sample group, with 6 replicates in each group. Using complete medium as the diluent, prepare sample working solutions with different concentrations according to the sample test concentration table, 100 μl / well. Add an equal amount of complete medium to the blank control group, and place it in an incubator at 37 °C with 5% CO 2 Cultivate for 24 h. Detect the content of β-endorphin in the test substance by ELISA method. Measure the absorbance (OD value) with an enzyme-labeled instrument at a wavelength of 450 nm. Calculate the content of human β-endorphin in the sample through the standard curve. At the same time, use GraphPad Prism 8.0 software for data statistical analysis and draw charts. Measurement data are expressed as x±s, and one-way ANOVA is used for analysis of differences between groups. A P value < 0.05 is considered statistically significant difference.

[0097] 1.3 Test results and analysis

[0098] The data of the β-endorphin release rate in cells after adding the compositions of the examples and comparative examples are shown in Table 3 and Figure 1 as shown. Comparative example 4 solely verifies the promoting effect of jasmine flower extract on the β-endorphin release rate. Examples 4-11 are a four-factor three-level orthogonal test. The experimental results show that the single jasmine flower extract can promote the release of β-endorphin in Hacat cells. The four raw materials as a composition have a synergistic effect. According to the range analysis, compared with the known jasmine flower extract, citrus fruit extract also shows a good effect on promoting β release. The order of raw materials that play an important role in releasing endorphin is citrus fruit extract, jasmine flower extract, fucus extract, and camellia seed oil. Among them, the preferred addition amount of citrus fruit extract is 0.5%, the preferred addition amount of jasmine flower extract is 0.05%, the preferred addition amount of fucus extract is 0.3%, and the preferred addition amount of camellia seed oil is 0.5%. The finally selected ratio is used in Example 1. Compared with the blank control, the β-endorphin release rate of Example 1 is 4.91%, which has a better ability to promote endorphin release and is more likely to help consumers relieve stress and enhance the sense of pleasure.

[0099] Table 3 β-endorphin release rate in cells after adding the compositions of the examples and comparative examples

[0100]

[0101] 2. Evaluation of the composition's promotion of microcirculation: Heme oxygenase 1 (HO-1) activation test experiment

[0102] 2.1 Test Principle

[0103] Heme oxygenase (HO)-1 and its enzymatic hydrolysis products carbon monoxide, ferritin, and bilirubin can maintain microcirculation stability by reducing reactive oxygen species damage, reducing apoptosis, and inhibiting inflammatory responses. The stimulating activity of the sample on heme oxygenase 1 (HO-1) was determined by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). The specific ELISA experimental principle is the same as above.

[0104] 2.2 Test Method

[0105] Adjust the density of the human immortalized keratinocyte HaCat suspension to 15,000 cells / well and then inoculate it into a 96-well plate, 100 μL / well, and place it in an incubator at 37°C with 5% CO 2 and culture for 24 h. Set up a blank control group (BC) and a sample group. There are 3 concentration gradients in the sample group, and 6 replicates are set for each group. Using complete medium as the diluent, prepare different concentrations of sample working solutions according to the sample test concentration table, 100 μl / well. The blank control group is added with an equal amount of complete medium, and place it in an incubator at 37°C with 5% CO 2 After culturing for 24 h, observe the cell morphology under a microscope and detect the HO-1 content by ELISA.

[0106] 2.3 Test Results and Analysis

[0107] The data of the HO-1 concentration in cells after adding the compositions of the examples and comparative examples are shown in Table 4 and Figure 2As shown. Comparative Example 1, Comparative Example 2, and Comparative Example 3 verified the ability of a single raw material to promote the upregulation of HO-1 in cells. The experimental results showed that the selected raw materials could all promote the upregulation of HO-1 to a certain extent, which was helpful for promoting blood microcirculation. Examples 4-11 were a set of orthogonal experiments with four factors and three levels to verify the importance degree and appropriate dosage of four preferred raw materials in promoting the upregulation of HO-1 in cells. According to the range analysis, the extract of Fucus vesiculosus showed a more excellent effect in promoting the upregulation of HO-1, followed by the extract of citrus fruit and camellia seed oil in turn. The extract of Jasminum grandiflorum had no obvious effect. The compound use of the extract of Fucus vesiculosus, the extract of citrus fruit, and camellia seed oil had a synergistic effect in promoting the upregulation of HO-1 in cells. The addition amount of the extract of Fucus vesiculosus was preferably 0.3% and 0.5%, and 0.3% was preferably selected based on the principle of maximum utilization. The addition amount of the extract of citrus fruit was preferably 0.5%, and the addition amount of camellia seed oil was preferably 0.5%. The finally selected ratio was applied to Example 1 in combination with other experimental results. Compared with the blank control with an HO-1 concentration of 1.55 ng / ml, the HO-1 concentration in cells was upregulated to 5.67 ng / ml in Example 1, which had a better effect on promoting blood microcirculation; at the same time, compared with Example 5 with a composition content of 1.4% with similar ability, the HO-1 concentration in cells was upregulated to 5.56 ng / ml, and in Example 11 with a composition content of 1.85%, the HO-1 concentration in cells was upregulated to 5.77 ng / ml. The composition content of Example 1 was 1.35%, maximizing the utilization of raw materials to achieve excellent results.

[0108] Table 4 HO-1 concentration in cells after adding the compositions of the examples and comparative examples

[0109]

[0110] 3. Evaluation of the repair efficacy of the composition: Cell scratch assay

[0111] 3.1 Test principle

[0112] The cell scratch assay is a simple in vitro test method for studying cell migration. When the skin surface is damaged, keratinocytes will be stimulated to migrate to repair the damaged part. When the cells grow to a monolayer state in vitro, an artificial blank area is created on the monolayer cells. The cells at the edge of the scratch will gradually enter the blank area to heal the scratch, which to a certain extent simulates the in vivo cell migration process. By calculating the area or width of the scratch to semi-quantitatively calculate the healing percentage of the scratch, the migration ability of the cells can be judged, indirectly reflecting the repair efficacy of the test substance.

[0113] 3.2 Test method

[0114] Use sterilized tweezers to place the bottom of the culture-insert 2well insert in a 6-well plate. Inoculate 60,000 HaCat cells per well in the wells on both sides of the culture-insert 2well and place at 37°C, 5% CO 2 Incubate in the incubator for 24 hours. According to the test plan, pull out the scratch plug, take pictures, and divide the drugs into groups, with 2 replicate wells in each group. After drug administration, place the 6-well plate in the incubator (37°C, 5% CO 2 ) and cultured for 24 h. The cell morphology was observed under a microscope and photographed. ImageJ software was used for image statistical area analysis, and GraphPad Prism 8 software was used for data statistical analysis. The calculation formula was: (0H scratch blank area area-24H scratch blank area area) / 0H scratch blank area area*100.

[0115] 3.3 Test results and analysis

[0116] The cell scratch test data of the embodiment and the comparative example are shown in Table 5 and Figure 3 As shown, Figure 4 The diagrams are the cell scratch healing effects of the embodiments and comparative examples.

[0117] The process of assisting skin microcirculation is also the process of promoting keratinization or accelerating fibroblast renewal metabolism, which can effectively promote cell regeneration. The cell healing ability can reflect the promoting effect of the composition on skin microcirculation to a certain extent. The comparative examples all show a certain cell migration growth rate, indicating that the selected raw materials have a certain repair effect, among which the comparative example 2 has the best scratch healing effect. At the same time, relative to a single raw material, the preferred composition has a more excellent healing effect, and the effects of all embodiments are superior to the comparative examples, with significant differences, indicating that the combination of the composition can synergize, but the higher the raw material concentration, the better. In Example 3, when the amount of raw material added is more, it does not show a better repair effect, and the scratch healing percentage is 40.88%. Among all the embodiments, Example 1 is the best example, and the scratch healing percentage reaches 46.5%, which has the best repair effect.

[0118] Table 5 Cell scratch test data of Examples and Comparative Examples

[0119]

[0120] 4. Finished product promotion microcirculation evaluation: nail fold microcirculation detection experiment

[0121] 4.1 Test Principle

[0122] The nail fold refers to the skin fold covering the base of the nail. Its surface is covered by squamous epithelium, and inside it are papillae formed by dermal protrusions. Each papilla contains one to several capillaries, which are loop-shaped and have the simplest capillaries in the human body. The nail fold microcirculation test can quantitatively analyze the observed indicators such as the number of microvessels, diameter, flow rate, etc. and comprehensively evaluate the microcirculation status. It is an important indicator reflecting the microcirculation perfusion status of the body, and to a certain extent reflects the macrocirculation status of various tissues, organs and systems of the body.

[0123] 4.2 Test Method

[0124] After cleaning the fingernail folds, apply about 0.1g of the test substance to the nail seam of the left ring finger. After slowly massaging in circles for 20 times, place the left ring finger on the observation paper tray of the microcirculation detector, so that the nail seam is directly under the light source, and adjust the lens until the picture is clear before observation.

[0125] 4.3 Test Results and Analysis

[0126] Test result pictures Figure 5 As shown. The nail folds have abundant microvessels and are sensitive to various stimuli. Under normal observation, blood can be seen flowing rapidly in the terminal capillaries under the microcirculation detection lens, which appears as rows of red capillaries as a whole. Both blood flow velocity and tube loop clarity can reflect the state of blood microcirculation to a certain extent. After using both Comparative Example B and Comparative Example A, multiple capillaries with rapidly flowing blood can be observed, but the tube loop clarity observed after using Comparative Example B is better. Compared with the comparative example, the makeup remover cream of Example A makes the application process smoother due to the oil base of the product. After gently massaging in circles for 20 times, a slight warmth can be felt. Further observation also shows that the blood flow velocity is fast and the capillaries are clearer, indicating that the use of the composition in an oil-based makeup remover product can achieve a better effect of promoting microcirculation.

[0127] 5. Finished product stress relief evaluation: pleasure test

[0128] 5.1 Test Principle

[0129] The volunteers were blindly asked to answer a questionnaire after using the samples continuously, wherein the test samples were Example A, Comparative Example A and Comparative Example B; 60 subjects were from the population with high perceived stress, prone to insomnia (more than 3 times a week of insomnia), had the habit of cleaning and skin care before going to bed, aged 18-55 years old, and the male-female ratio was 5:5.

[0130] 5.2 Test Method

[0131] All the subjects were randomly divided into three groups and were respectively used Example A, Comparative Example A and Comparative Example B. Before cleaning and skin care at night, a certain amount of the test sample was taken on the face of the subjects and applied by massaging in a circular motion for no less than 20 circles. Subsequently, personal normal cleaning and skin care could be carried out. It was used three times a week for four consecutive weeks. In this test, other possible effects of the product such as cleaning and moisturizing were not evaluated, and only the feelings of the subjects were concerned. The specific scoring criteria are shown in Table 6 below.

[0132] Table 6 Trial Scoring Criteria

[0133]

[0134] 5.3 Test Results and Analysis

[0135] The evaluations after use of 60 people were collected, and the specific statistics are shown in Table 7 below.

[0136] Table 7 Feedback of Test Subjects Using Samples of Example and Comparative Example

[0137]

[0138]

[0139] From the data statistical analysis, the subjects thought that none of the three samples had an inhibitory effect. Among the subjects using the sample of Comparative Example A, 35% of them thought it was helpful for relieving stress and enhancing the sense of pleasure, and 65% thought it was not helpful; among the subjects using Comparative Example B, 75% of them thought it was helpful for relieving stress and enhancing the sense of pleasure, but only 10% of them thought it had a significant effect, and 25% thought it was not helpful. The effect of Comparative Example B in emulsion dosage form was better than that of Comparative Example A in aqueous dosage form. Compared with the comparative examples, 85% of the subjects thought that the sample of Example A used was helpful for relieving stress and enhancing the sense of pleasure, and 40% of the subjects thought it had an obvious help; it shows that the composition of the present application has a more excellent effect when applied in an oil-based makeup remover product. Using a high-oil-content paste as the carrier makes the rubbing and massaging process smoother and more pleasant, better promotes skin microcirculation, relieves skin stress, and thus relaxes the subjects.

[0140] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit it; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

[0141] In addition, those skilled in the art will understand that although some embodiments herein include certain features included in other embodiments but not others, the combination of features of different embodiments means that it is within the scope of the present application and forms different embodiments. For example, in the claims above, any one of the claimed embodiments can be used in any combination. The information disclosed in this background section is only intended to enhance the understanding of the overall background art of the present application and should not be regarded as an admission or any form of implication that this information constitutes the prior art already known to those skilled in the art.

Claims

1. A composition for promoting skin microcirculation, characterized in that: Calculated by mass percentage, the composition comprises: 0.1%-1% of a component inducing vasodilation, 0.1%-1% of a component improving vascular tolerance, 0.06%-0.6% of a component reducing free hemoglobin, and 0.01%-0.3% of a component relieving perceived stress.

2. The composition for promoting skin microcirculation according to claim 1, characterized in that: The vasodilation-inducing component comprises: any one of safflower extract, camellia seed oil, bitter orange oil, ginkgo leaf extract, and green tea extract; Preferably, the vasodilation-inducing component comprises: camellia seed oil.

3. The composition for promoting skin microcirculation according to claim 2, characterized in that: The component for improving vascular tolerance includes: any one of citrus fruit extract, horse chestnut extract, Centella asiatica extract, bilberry extract, and Sophora japonica extract; Preferably, the component for improving vascular tolerance includes: citrus fruit extract.

4. The composition for promoting skin microcirculation according to claim 3, characterized in that: The free hemoglobin reducing component comprises any one of: Fucus vesiculosus extract, Crataegus monogyna flower extract, and Gardenia jasminoides fruit bud extract; Preferably, the free hemoglobin reducing component comprises: Fucus vesiculosus extract.

5. The composition for promoting skin microcirculation according to claim 4, characterized in that: The ingredient for relieving perceived stress includes: any one of frangipani flower extract, biosaccharide gum-2, saffron extract, lemon balm extract, and edamame seed extract; Preferably, the ingredient for relieving perceived stress includes: frangipani flower extract.

6. The composition for promoting skin microcirculation according to claim 5, characterized in that: The composition comprises, by mass percentage, 0.1%-1% of camellia seed oil, 0.1%-1% of citrus fruit extract, 0.06%-0.6% of fucus vesiculosus extract and 0.01%-0.3% of frangipani flower extract; Optionally, the composition comprises, by mass percentage: 0.5%-1% of camellia seed oil, 0.5%-1% of citrus fruit extract, 0.3%-0.6% of fucus vesiculosus extract and 0.05%-0.3% of frangipani extract.

7. A makeup removing cream, characterized in that: A composition for promoting skin microcirculation comprising any one of claims 1 to 6.

8. The makeup removing cream according to claim 7, characterized in that: In terms of mass percentage, it also includes: 50%-90% of an emollient, 5%-25% of a nonionic emulsifier, 4%-8% of an oil phase thickener and 0.1-0.5% of a preservative.

9. A method for preparing the makeup remover cream according to claim 8, characterized in that: include: The emollient, nonionic emulsifier and oil phase thickener are added into a container, stirred and heated until the materials are completely melted, cooled, and the composition for promoting skin microcirculation and preservative are added in sequence.

10. The method for preparing the makeup removing cream according to claim 9, characterized in that: The stirring speed is 300-500 rpm, the heating temperature is 85±5°C, and the temperature is cooled to 55-60°C.

Images