Shallow liquid flow self-circulation seedling raising method for lily breeding
Through the shallow liquid flow self-circulation seedling cultivation method, the use of microporous stone and self-circulating water bodies, the problems of insufficient soil pests and hydroponics in lily breeding are solved, and the reproduction effect is achieved with high success rate and low cost.
Patent Information
- Application Number
- CN202510357521.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-25
- Publication Date
- 2025-06-10
AI Technical Summary
The existing lily reproduction technology has problems such as soil pests and diseases, high production costs, low reproduction success rate, and insufficient hydroponic oxygen to cause scale decay.
The shallow liquid flow self-circulation seedling cultivation method is used, and microporous stones are used as the substrate to form a shallow liquid flow self-circulation water body, control temperature and humidity, disinfect scales and place them on the stone bed surface to achieve moderate humidity of the scales and healthy growth of the root system.
It significantly improves the success rate of lily reproduction, reduces production costs, avoids scale rot, and is relatively easy to control temperature and humidity, which is suitable for large-scale promotion.
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of soilless cultivation, and in particular to a shallow liquid flow self-circulating seedling raising method for lily propagation. Background Art
[0002] Liliaceae plants are very lovely. When planted, their colors, petals and fragrance are quite charming and their economic value is huge. The fleshy leaves of lily bulbs are used to store food, which can make the plants dormant when there is a lack of water (such as in winter or drought) and revived when conditions are favorable. The fleshy leaves are actually enlarged leaf bases, and the storage leaves are exposed. The appearance seems to be composed of many angular scales. The bulbs vary in size, and the small ones are as small as peas. Lilies are planted in a large area because of their delicious fleshy leaves and rich nutrition, which are of great economic value to humans.
[0003] The continuation of lily involves reproduction. The bulb has asexual reproduction ability and can directly develop small bulbs. Lily has the advantages of growing faster from bulbs than from seeds. The scales of lily are cut and the adaxial surface tissue at the base of the scales forms microbulbs. After the microbulbs are formed, they are transplanted into suitable soil or matrix to promote bulb formation and form commercial products. At present, whether at home or abroad, the lily breeding process is generally carried out in the field or indoors. After data research and actual visits, it is found that the disadvantage of field breeding is that there are pests and diseases in the soil, resulting in large-scale use of pesticides and residues that seriously affect green production. In addition, the large amount of water, fertilizer and herbicide input results in high production management costs and long production cycles. Indoor breeding mainly adopts matrix cutting technology, such as patent CN107889630A, which has the disadvantage that the scale change state cannot be monitored in real time. As a result, the scales partially buried in the matrix rot, affecting the breeding rate. Furthermore, rotten scales also affect the continued use of the cutting medium, which requires disinfection, which increases costs and prolongs the propagation time. Therefore, the general success rate of traditional propagation is only 50%-70%.
[0004] In order to improve the defects of field and greenhouse soil and matrix propagation technology, patent CN104145797B uses hydroponics to propagate lily scales. However, lilies are aerobic plants and their cell activities need to breathe oxygen in the air. Immersing the top of the scales, i.e. the cut part of the scales, in water can easily cause the scale cells to breathe poorly, which is not conducive to the formation of bulbs. In severe cases, it can also cause rot due to lack of oxygen, which is not easy to promote. Patent application CN116098050A is actually also a hydroponic anaerobic environment, which also has the following shortcomings: (1) The closed device and hydroponic environment can also cause insufficient oxygen and scale rot; (2) The device structure is complex, and the operation and maintenance of various sensors increase the propagation cost, and the operation threshold is high, which is not conducive to actual production. Patent CN104106459B provides a "suspended hydroponic seedling method of lily scales". In order to overcome the lack of oxygen supply, activated carbon is added to the sponge roll. The core is to roll the sponge into a cylinder, tie it at 2 / 3, put the other 1 / 3 end into a container of distilled water for capillary water absorption, and use the sponge to absorb water and conduct the water to the activated carbon, but it is also necessary to spray nutrient solution on the scales of the activated carbon every day for the growth and rooting of lilies. This method is complicated, such as the sponge standing up, filling, cutting at a height of 2 / 3, spraying water, etc. It is inconvenient to operate, easily affected by unexpected factors, resulting in unstable effects, and requires the use of expensive activated carbon. Therefore, the labor cost and material cost of this method are high, the preparation method and operation method of the culture device are complicated, and it is difficult to apply it in large-scale production. Summary of the invention
[0005] In order to overcome the defects of the current lily substrate cutting and hydroponic propagation technology, the present invention provides a shallow liquid flow self-circulation seedling raising method for lily propagation, and the seedling raising method comprises the following steps:
[0006] (1) Decontaminated microporous stones with a particle size of 3-6 mm are piled on the seedbed to form a bed surface with a height of 3-10 cm;
[0007] (2) The rooting liquid forms a shallow liquid self-circulating water body in the seedling bed that is 3-8 mm below the bed surface formed by the stones;
[0008] (3) After disinfecting and drying the lily bulb scales, place them on the stone bed with the concave side facing upwards;
[0009] (4) Cultivate at a temperature of 22-25°C and a relative humidity of 50%-60% until the scales grow into bulbs and roots.
[0010] In the step (1), the microporous stone is disinfected with a 300-fold dilution of carbendazim for 15 minutes and then rinsed with sterile water; the microporous stone is selected from at least one of zeolite, volcanic rock and diatomaceous earth;
[0011] The seedbed is 500 cm long, 150 cm wide and 5-10 cm deep.
[0012] In step (2), the rooting solution is a full-element nutrient solution, an agricultural rare earth solution, or a mixture of a full-element nutrient solution and agricultural rare earth nitrate; wherein the full-element nutrient solution contains 180 mg / L KH 2 PO 4 、30mg / L KNO 3 、200mg / L Ca(NO 3 ) 2 , 187mg / LK 2 SO 4 、47mg / L MgSO 4 、800mg / L NH 4 NO 3 , 14.31mg / L chelated iron, 0.84mg / L ZnSO 4 、0.13mg / L CuSO 4 , 2.54mg / LH 3 BO 3 、0.52mg / L(NH 4 ) 6 Mo 7 O 24 ·4H 2 O, 0.5mg / L MnSO 4 ·H 2 O; the concentration of the agricultural rare earth nitrate solution is 0.1-0.5g / L; the agricultural rare earth nitrate contains at least one of lanthanum nitrate and cerium nitrate; the rooting solution composed of a mixture of full-element nutrient solution and agricultural rare earth nitrate is prepared by adding agricultural rare earth nitrate to the full-element nutrient solution at a concentration of 0.1-5g / L.
[0013] In the step (2), rooting liquid is pumped into one end of the seedbed at a regular time, and the other end of the seedbed is opened for the rooting liquid to flow out and flow back into the container containing the rooting liquid, thereby forming the shallow liquid flow self-circulating water body; the rooting liquid circulates once every 1-15 days, and each time is 0.1-0.5 hours.
[0014] In the step (3), lily bulbs with a diameter of 3-5 cm that are healthy, free of pests and diseases, and have full scales are selected, and the scales of the lily bulbs are carefully peeled off with a sharp and sterilized knife, each scale having a portion of the bulb disc tissue; the bulb scales of the lily bulbs are disinfected with a 300-fold dilution of carbendazim for 15-20 minutes, and after the surface moisture is dried, the bulbs are placed on the bed with the concave surface facing upwards, end to end.
[0015] The method also includes replacing the scales in time when signs of rotting are observed; and transplanting the scales to other nutrient media after the scales grow bulbs and hair roots of more than 1 cm.
[0016] The beneficial effects of the present invention include:
[0017] (1) The present invention uses microporous stone as a matrix and utilizes natural capillary pore stone to provide lily scales with a relatively moist environment that is neither waterlogged nor water-deficient. This environment is most suitable for the germination of scale roots and the formation of bulbs, and can reduce scale decay. In addition, because the stone particles are more thoroughly disinfected than the matrix or sand and are not affected by herbicides, the disinfection is simple and thorough, further reducing the decay of the scales.
[0018] (2) Temperature and humidity are easy to control. Since the scales are not immersed in water or buried in the matrix, the temperature and humidity of the scales themselves can be easily controlled by placing the seedbed of the present invention in a relatively closed space (plastic arch shed).
[0019] (3) The cultivation method of the present invention realizes visualization of the cultivation process. When scales are found to be rotting, they can be replaced in time, thus avoiding the waste of space and breeding resources.
[0020] (4) The seedling raising method of the present invention does not require complicated equipment, and the raw materials such as the bed, stone, and water pump are cheap and reusable, which reduces the production and maintenance costs. When raising seedlings, the scales can be placed on the bed formed by the stone. The operation is simple and easy, and suitable for large-scale promotion.
[0021] (5) The seedling raising method of the present invention significantly improves the breeding success rate (the average value of multiple sample tests is over 80%). DETAILED DESCRIPTION
[0022] The present invention is further illustrated and described below in conjunction with the embodiments, but the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the present invention and the embodiments, all other inventions and embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present invention.
[0023] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.
[0024] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.
[0025] Example 1: Shallow liquid flow self-circulation seedling raising method for lily propagation
[0026] Preparation of seedling bed: In the temperature and humidity adjustable greenhouse of the Institute of Plant Nutrition and Environment of Beijing Academy of Agricultural and Forestry Sciences, zeolite with a diameter of 3-4 mm was soaked in a 300-fold dilution of carbendazim for 15 minutes, then taken out and dried. The sterilized zeolite was placed on a custom-made seedling bed with a length of 500 cm, a width of 150 cm, and a depth of 5 cm, and the zeolite particles on the seedling bed were spread to form a bed surface with a thickness of 4 cm.
[0027] Preparation of rooting solution: press KH 2 PO 4 (180mg / L), KNO 3 (30mg / L), Ca(NO 3 ) 2 (200mg / L), K 2 SO 4 (187mg / L), MgSO 4 (47mg / L), NH 4 NO 3 (800mg / L), chelated iron (14.31mg / L), ZnSO 4 (0.84mg / L), CuSO 4 (0.13mg / L), H 3 BO 3 (2.54mg / L), (NH 4 ) 6 Mo 7 O 24 ·4H 2 O (0.52 mg / L), MnSO 4 ·H 2 O (0.5 mg / L) is added to prepare a full-element nutrient solution, and lanthanum nitrate is added to the full-element nutrient solution at a concentration of 0.1 g / L to obtain a rooting solution. A total of 350 liters of the rooting solution is set aside.
[0028] Lily cultivation:
[0029] (1) Select lily bulbs that are healthy, free of pests and diseases, and have full scales. The diameter of the bulbs is 3-5 cm. Bulbs that are too small have insufficient nutritional reserves, which may affect the success rate of reproduction and the growth potential of new plants; bulbs that are too large may be more expensive and relatively inconvenient to operate. Use a sharp and sterilized knife to carefully peel off the scales of the lily bulbs. When peeling the scales, try to keep the scales intact and avoid damage. Start peeling from the outer layer of the bulb. Each scale has a part of the bulb disk tissue, which is very important for the rooting and germination of the scales. Soak the peeled scales in a 300-fold dilution of carbendazim for 15 minutes for disinfection to prevent bacterial infection. Take out after soaking and place in a cool and ventilated place to dry the surface moisture for later use.
[0030] (2) Use a water pump with a water pumping capacity of 30 L / min to pump rooting liquid onto the seedbed to form a water body 2 mm below the stone bed surface. Pump rooting liquid once every 10 days and replenish it at one end of the seedbed for 12 minutes each time. Open the other end of the seedbed to allow the rooting liquid to flow down to form a circulation system.
[0031] (3) After disinfection, place the scales end to end on the stone bed with the concave side facing upward.
[0032] (4) Keep the scales at 25°C and 60% relative humidity. During this period, observe the condition of the scales in real time and replace scales that tend to mold quickly. After the scales have grown roots of 1 cm (40 days), remove them and transplant them to a substrate suitable for their growth environment for further cultivation.
[0033] Example 2: Shallow liquid flow self-circulation seedling raising method for lily propagation
[0034] Preparation of seedling bed: In the temperature and humidity adjustable greenhouse of the Institute of Plant Nutrition and Environment of Beijing Academy of Agricultural and Forestry Sciences, volcanic rock with a diameter of 5-6 mm was used, soaked in a 300-fold dilution of carbendazim for 15 minutes, fished out and dried. The sterilized volcanic rock was placed on a custom-made seedling bed with a length of 500 cm, a width of 150 cm and a depth of 10 cm, and the volcanic rock particles on the seedling bed were spread into a bed surface with a thickness of 10 cm.
[0035] Preparation of rooting solution: Prepare agricultural rare earth nitrate as rooting solution according to the concentration of 0.5g / L of lanthanum nitrate and cerium nitrate respectively, with a total of 350 liters of rooting solution for use.
[0036] Lily cultivation:
[0037] (1) Select lily bulbs that are healthy, free of pests and diseases, and have full scales. The diameter of the bulbs should be 3-5 cm. Use a sharp and sterilized knife to carefully peel off the scales of the lily bulbs. Try to keep the scales intact when peeling and avoid damage. Start peeling from the outer layer of the bulb. Each scale has a part of the bulb disc tissue. Soak the peeled scales in a 300-fold dilution of carbendazim for 20 minutes to disinfect and prevent bacterial infection. Take out after soaking and place in a cool and ventilated place to dry the surface moisture for later use.
[0038] (2) Use a water pump with a water pumping capacity of 25 L / min to pump rooting liquid onto the seedbed to form a water body 2 mm below the bed surface formed by the stones. Pump the rooting liquid once every 15 days and replenish it at one end of the seedbed. Each pumping takes 30 minutes. The other end of the seedbed is opened to allow the rooting liquid to flow down to form a circulation system.
[0039] (3) After disinfection, place the scales end to end on the stone bed with the concave side facing upward.
[0040] (4) Keep the scales at 25°C and 50% relative humidity. During this period, observe the condition of the scales in real time and replace scales that are prone to mold immediately. After the scales have formed balls and grown roots (39 days), remove them and transplant them to a substrate suitable for their growth environment for further cultivation.
[0041] Example 3: Shallow liquid flow self-circulation seedling raising method for lily propagation
[0042] Preparation of nursery bed: In the greenhouse with adjustable temperature and humidity of the Institute of Plant Nutrition and Environment of Beijing Academy of Agricultural and Forestry Sciences, diatomaceous earth with a diameter of 3-6 mm was used, soaked in a 300-fold dilution of carbendazim for 15 minutes, fished out and dried. The sterilized stone was placed on a custom-made nursery bed with a length of 500 cm, a width of 150 cm and a depth of 6 cm, and the diatomaceous earth particles on the nursery bed were spread into a bed surface with a thickness of 5 cm.
[0043] Preparation of rooting solution: press KH 2 PO 4 (180mg / L), KNO 3 (30mg / L), Ca(NO 3 ) 2 (200mg / L), K 2 SO 4 (187mg / L), MgSO 4 (47mg / L), NH 4 NO 3 (800mg / L), chelated iron (14.31mg / L), ZnSO 4 (0.84mg / L), CuSO 4 (0.13mg / L), H 3 BO 3 (2.54mg / L), (NH 4 ) 6 Mo 7 O 24 4H2O (0.52mg / L), MnSO 4 ·H 2 O (0.5 mg / L) was added to prepare a full-element nutrient solution, and lanthanum nitrate and cerium nitrate were added to the full-element nutrient solution at a concentration of 2.5 g / L to obtain a rooting solution. A total of 375 liters of the rooting solution was set aside.
[0044] Lily cultivation:
[0045] (1) Select lily bulbs that are healthy, free of pests and diseases, and have full scales. The diameter of the bulbs should be 3-5 cm. Use a sharp and sterilized knife to carefully peel off the scales of the lily bulbs. Try to keep the scales intact when peeling and avoid damage. Start peeling from the outer layer of the bulb. Each scale has a part of the bulb disc tissue. Soak the peeled scales in a 300-fold dilution of carbendazim for 20 minutes to disinfect and prevent bacterial infection. Take out after soaking and place in a cool and ventilated place to dry the surface moisture for later use.
[0046] (2) Use a water pump with a water pumping capacity of 65 L / min to pump rooting liquid onto the seedling bed to form a water body 2 mm below the bed surface formed by the stones. Then regularly pump rooting liquid once a day to replenish one end of the seedling bed. Each pumping lasts for 6 minutes. The other end of the bed is opened to allow the rooting liquid to flow down to form a circulation system.
[0047] (3) After disinfection, place the scales end to end on the stone bed with the concave side facing upward.
[0048] (4) Keep the scales at 24°C and the relative humidity at 55%. During this period, observe the condition of the scales in real time and replace scales that are prone to mold immediately. After the scales have formed balls and grown roots (35 days), remove them and transplant them to a substrate suitable for their growth environment for further cultivation.
[0049] Example 4: Shallow liquid flow self-circulation seedling raising method for lily propagation
[0050] Preparation of nursery bed: In the temperature and humidity adjustable greenhouse of the Institute of Plant Nutrition and Environment of Beijing Academy of Agricultural and Forestry Sciences, a mixture of zeolite with a diameter of 3-6mm and volcanic rock with a volume ratio of 1:1 was used, soaked in a 300-fold dilution of carbendazim for 15 minutes, then fished out and dried. The sterilized stones were placed on a custom-made nursery bed with a length of 500cm, a width of 150cm and a depth of 8cm, and the zeolite + volcanic rock material on the nursery bed was spread to a bed surface with a thickness of 6cm.
[0051] Preparation of rooting solution: Prepare 5g / L agricultural rare earth lanthanum nitrate solution as rooting solution, with a total of 375 liters of rooting solution for use.
[0052] Lily cultivation:
[0053] (1) Select lily bulbs that are healthy, free of pests and diseases, and have full scales. The diameter of the bulbs should be 3-5 cm. Use a sharp and sterilized knife to carefully peel off the scales of the lily bulbs. Try to keep the scales intact when peeling and avoid damage. Start peeling from the outer layer of the bulb. Each scale has a part of the bulb tissue. Soak the peeled scales in a 300-fold dilution of carbendazim for 15 minutes to disinfect and prevent bacterial infection. Take out after soaking and place in a cool and ventilated place to dry the surface moisture for later use.
[0054] (2) Use a water pump with a water pumping capacity of 20 L / min to pump rooting liquid onto the seedling bed to form a water body 4 mm below the bed surface formed by the stones. After a certain time, pump the rooting liquid once every 5 days to replenish it at one end of the seedling bed. Each time, pump for 20 minutes. Open the other end of the bed to allow the rooting liquid to flow down to form a circulation system.
[0055] (3) After disinfection, place the scales end to end on the stone bed with the concave side facing upward.
[0056] (4) Keep the scales at 25°C and the relative humidity at 58%. During this period, observe the condition of the scales in real time and replace the scales that tend to mold quickly. After the scales form balls and grow roots (42 days), remove them and transplant them to a substrate suitable for their growth environment for further cultivation.
[0057] Example 5: Shallow liquid flow self-circulation seedling raising method for lily propagation
[0058] Preparation of nursery bed: In the greenhouse with adjustable temperature and humidity of the Institute of Plant Nutrition and Environment of Beijing Academy of Agricultural and Forestry Sciences, mixed stones with a diameter of 3-5cm, zeolite + volcanic rock + diatomaceous earth in a volume ratio of 1:1:1 were used, soaked in a 300-fold dilution of carbendazim for 15 minutes, then fished out and dried. The sterilized mixed stones were placed on a custom-made trough bed with a length of 500cm, a width of 150cm and a depth of 7cm, and the zeolite + volcanic rock + diatomaceous earth stones on the bed were laid to form a stone surface with a thickness of 5cm.
[0059] Preparation of rooting solution: prepare agricultural rare earth solution with lanthanum nitrate and cerium nitrate concentration of 2.5g / L respectively as rooting solution, with a total of 300L of rooting solution for standby use;
[0060] Lily cultivation:
[0061] (1) Select lily bulbs that are healthy, free of pests and diseases, and have full scales. The diameter of the bulbs should be 3-5 cm. Use a sharp and sterilized knife to carefully peel off the scales of the lily bulbs. Try to keep the scales intact when peeling and avoid damage. Start peeling from the outer layer of the bulb. Each scale has a part of the bulb disc tissue. Soak the peeled scales in a 300-fold dilution of carbendazim for 20 minutes to disinfect and prevent bacterial infection. Take out after soaking and place in a cool and ventilated place to dry the surface moisture for later use.
[0062] (2) Use a water pump with a water pumping capacity of 15 L / min to pump rooting liquid onto the seedling bed to form a water body 3 mm below the bed surface formed by the stones. Then regularly pump rooting liquid once every 8 days to replenish one end of the seedling bed. Each pumping takes 24 minutes. The other end of the bed is opened to allow the rooting liquid to flow down to form a circulation system.
[0063] (3) After disinfection, place the scales end to end on the stone bed with the concave side facing upward.
[0064] (4) Keep the scales at 23°C and the relative humidity at 53%. During this period, observe the condition of the scales in real time and replace scales that are prone to mold immediately. After the scales have formed balls and grown roots (38 days), remove them and transplant them to a substrate suitable for their growth environment for further cultivation.
[0065] Comparative example, conventional propagation method of lily
[0066] A. Conventional matrix seedbed cutting propagation method: Use the method of step (1) of lily cultivation in Example 1 to obtain lily scales. The matrix is composed of 22 parts perlite, 18 parts fine sand, 16 parts peat, 14 parts mushroom residue, 10 parts vermiculite, and 8 parts fermented corn stalks by weight, and is sterilized for standby use. Spread the matrix into a 5 cm thick seedbed with a moisture content of 25%. Cut the scales with the base facing down on the wet bed and cultivate them at a temperature of 25°C. When (42 days) small bulbs sprout from the base of the scales and the roots grow to 1 cm, they can be planted in nutrient-containing matrix soil.
[0067] B. Hydroponic cutting propagation method: Use the method of step (1) of lily cultivation in Example 1 to obtain lily scales, and immerse one end of the cut part of the scale in a hydroponic nutrient solution containing 10 mg / L calcium nitrate, 100 mg / L potassium nitrate, 25 mg / L diammonium phosphate, 8 mg / L magnesium sulfate and 1 mg / LEDTA-2NaFe. Propagate at an ambient temperature of 25°C and a relative humidity of 60%. When (75 days) small bulbs sprout from the base of the scales and the roots grow to 1 cm, they can be planted in a nutrient-rich substrate.
[0068] On May 24, 2024, lilies were propagated using the two conventional propagation methods of Examples 1-5 and the comparative example, respectively. 1,000 lily scale samples were collected for each treatment. The results are shown in Table 1.
[0069] Table 1. Culture results of different propagation methods
[0070] deal with Number of rooting leaves Success rate (%) Cost (yuan / piece) Example 1 820 82% 1.48 Example 2 785 78.5% 1.43 Example 3 830 83% 1.45 Example 4 830 83% 1.42 Example 5 792 79.2% 1.4 Conventional seedbed cuttings 661 66.1% 1.65 Hydroponic cuttings 699 69.9 1.59
[0071] As shown in Table 1, the survival rate of lily conventional propagation methods is less than 70%, of which the survival rate of conventional seedbed cuttings is about 66% and that of hydroponics is 70%. The main reason is that it is difficult to thoroughly disinfect the soil (matrix) in the seedbed for cuttings. In addition, half of the lily scales are in the soil (matrix) and the other half are in the air, and it is not easy to control the temperature and humidity. Therefore, rotting often occurs during reproduction, resulting in a low success rate and an increase in the reproduction cost. The hydroponic method immerses the top of the scale, i.e., the cut part of the scale, in water, which can easily cause poor respiration of the scale cells, is not conducive to the formation of bulbs, and can also cause rot due to long-term immersion. It can be seen from the culture results of Examples 1-5 in Table 1 that, regardless of whether a single or multiple porous mixed stones are used, regardless of whether the stone bed is thick or thin, regardless of whether a nutrient solution type rooting solution or a nutrient solution and rare earth mixed solution is used, or even only agricultural rare earth rooting solution is used, the reproduction success rate of the seedling raising method of the present application is significantly improved compared with the prior art, with an average success rate of more than 80%, and a cost reduction of 10.3%-15.2%. The main reason is that the seedling bed of the present invention is relatively easy to disinfect, the temperature and humidity are easy to control, the porous infiltration self-circulating root-promoting liquid stimulates the growth of the root system, and coupled with the visual detection and replacement measures for rot, the comprehensive synergistic technology leads to a significant improvement in the breeding success rate and cost reduction and efficiency improvement compared with conventional breeding systems.
Claims
1. A shallow liquid flow self-circulating seedling raising method for lily propagation, characterized in that: The seedling raising method comprises the following steps: (1) Decontaminated microporous stones with a particle size of 3-6 mm are piled on the seedbed to form a bed surface with a height of 3-10 cm; (2) The rooting liquid forms a shallow liquid self-circulating water body in the seedling bed that is 3-8 mm below the bed surface formed by the stones; (3) After disinfecting and drying the surface moisture of the lily bulb scales, place them on the stone bed with the concave side facing upwards; (4) Cultivate at a temperature of 22-25°C and a relative humidity of 50%-60% until the scales grow into bulbs and roots.
2. The seedling raising method according to claim 1, characterized in that: In the step (1), the microporous stone is disinfected with a 300-fold dilution of carbendazim for 15 minutes and then rinsed with sterile water; and / or the microporous stone is selected from at least one of zeolite, volcanic rock and diatomaceous earth.
3. The seedling raising method according to claim 1, characterized in that: In the step (2), the rooting solution is a full-element nutrient solution, an agricultural rare earth nitrate solution, or a mixture of a full-element nutrient solution and agricultural rare earth nitrate.
4. The seedling raising method according to claim 3, characterized in that: Includes one or more of the following (1)-(4): (1) The full element nutrient solution contains 180 mg / L KH2PO4, 30 mg / L KNO3, 200 mg / L Ca(NO3)2, 187 mg / L K2SO4, 47 mg / L MgSO4, 800 mg / L NH4NO3, 14.31 mg / L chelated iron, 0.84 mg / L ZnSO4, 0.13 mg / LCuSO4, 2.54 mg / L H3BO3, 0.52 mg / L (NH4)6Mo7O 24 ·4H2O, 0.5mg / L MnSO4·H2O; (2) The concentration of the agricultural rare earth nitrate solution is 0.1-0.5 g / L; (3) The agricultural rare earth nitrate comprises at least one of lanthanum nitrate and cerium nitrate; (4) The rooting solution composed of a mixture of a full-element nutrient solution and agricultural rare earth nitrate is prepared by adding agricultural rare earth nitrate to the full-element nutrient solution at a concentration of 0.1-5 g / L.
5. The seedling raising method according to claim 1, characterized in that: In the step (2), rooting liquid is pumped into one end of the seedbed at a regular time, and the other end of the seedbed is opened for the rooting liquid to flow out and flow back into the container containing the rooting liquid, thereby forming the shallow liquid flow self-circulating water body.
6. The method for raising seedlings according to claim 5, characterized in that: The rooting solution should be circulated every 1-15 days, each time for 0.1-0.5 hours.
7. The seedling raising method according to claim 1, characterized in that: In the step (3), lily bulbs with a diameter of 3-5 cm that are healthy, free of pests and diseases, and have full scales are selected, and the scales of the lily bulbs are carefully peeled off with a sharp and sterilized knife, each scale having a portion of the bulb disc tissue; and / or, the bulb scales of the lily bulbs are disinfected with a 300-fold dilution of carbendazim for 15-20 minutes, and after the surface moisture is dried, the bulbs are placed on the bed with the concave surface facing upwards, end to end.
8. The seedling raising method according to claim 1, characterized in that: The method also includes replacing the scales promptly when signs of decay are observed.
9. The method for raising seedlings according to claim 1, characterized in that: The method also includes transplanting the scales into other nutrient media after the scales grow into bulbs and hair roots of more than 1 cm.
Citation Information
Patent Citations
Suspended Water Cultivation Seedling Method of Lily Scale
CN104106459B
A kind of method of hydroponic cutting of lily scale
CN104145797B
Scale cutting-seedling method for lilies
CN107889630A
Lily scale hydroponic seedling raising method and lily scale hydroponic seedling raising equipment
CN116098050A
Method for enlarging oriental lily scale cutting ball
CN102972198A
