Cell resuscitation instrument

Abstract

The invention discloses a cell resuscitation instrument which comprises a shell, a mounting frame is arranged in the shell, a plurality of resuscitation devices are arranged on the mounting frame at intervals, and each resuscitation device comprises a heating module and a heating cavity conducting heat through the heating module. The cell cryopreservation container is arranged in the heating cavity, and timing control and temperature control of cell resuscitation are realized through the main control unit; and a heat insulation device for blocking a heat source of the heated cell cryopreservation container is arranged in the heating cavity. According to the structure, a temperature control protection device is achieved through the temperature detection module and the main control unit, precise temperature control is achieved under the cooperation of the temperature detection module and the main control unit and the waste heat isolation device, multiple samples can be treated at the same time through the multiple heating modules arranged at intervals, the high-throughput experiment requirement is met, operation is simple in the whole cell recovery process, and the efficiency is high. The sample pollution is also avoided.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and particularly to a cell recovery instrument. Background Art

[0002] Cell recovery is an important step in the process of cell culture. Its purpose is to restore cryopreserved cells to a physiological state for subsequent experiments or applications. The traditional cell recovery method usually adopts the water bath method. Due to the high heat capacity ratio of water, it brings many inconveniences in the process of cell recovery, including slow heating and cooling speeds, inaccurate temperature control, and high energy consumption for heating. At the same time, the water bath method is cumbersome to operate and is also prone to contamination of the cell samples in the cryopreservation tubes due to improper operation. To solve the problems brought by the above water bath method in the process of cell recovery, the prior art also uses the hot air heating method. However, during the hot air heating process, due to the poor heat conduction ability of air, the heat transfer efficiency is low, the heating speed is slow, and the energy consumption is high. Moreover, the equipment has a large volume, and at the same time, using hot air heating is prone to noise pollution, etc. Whether it is the traditional water bath method or the hot air heating method adopted in the prior art, it is difficult to process multiple samples simultaneously, resulting in low experimental efficiency and unable to meet the requirements of high-throughput experiments. Summary of the Invention

[0003] To solve the above technical problems, the present invention provides a cell recovery instrument.

[0004] The technical solution of the present invention is: it includes a housing. A recovery device is provided in the upper space of the housing. The recovery device includes a heating module and a heating chamber that conducts heat through the heating module. A cell cryopreservation container is placed in the heating chamber, and the timing control and temperature control of cell recovery are realized through a main control unit. An insulating device for blocking the heat source of the cell cryopreservation container that has completed heating is provided in the heating chamber.

[0005] Its further technical solution is: the insulating device includes a top pin and a locking device. The top pin is driven to move through the elastic deformation of an elastic element. The bottom of the top pin passes through the bottom of the heating chamber and is connected to the locking device.

[0006] Its further technical solution is: the locking device includes a magnetic snap connected to the bottom of the top pin and an electromagnet spaced below the magnetic snap. When the magnetic snap moves down to close with the electromagnet, the main control unit controls the electromagnet to be energized and generate an electromagnetic force, and the electromagnet and the magnetic snap are firmly attracted to each other under the action of the electromagnetic force.

[0007] Its further technical solution is: a positioning sensor for detecting the position of the magnetic snap is provided on one side of the electromagnet, and the positioning sensor is electrically connected to the main control unit.

[0008] A further technical solution thereof is that: an installation rack is provided inside the housing, and a plurality of the resuscitation devices are arranged on the installation rack at intervals.

[0009] A further technical solution thereof is that: the installation rack includes an upper installation plate, a plurality of installation holes are provided on the upper installation plate, a vertically downward extending installation boss is provided at the lower end of the heating cavity, and the outer side wall of the installation boss is closely attached to the inner side wall of the installation hole.

[0010] A further technical solution thereof is that: a temperature detection module for real-time monitoring of the temperature inside the heating cavity is provided on the peripheral side of the heating cavity, and a plurality of the temperature detection modules are electrically connected to the main control unit.

[0011] A further technical solution thereof is that: the heating module includes a heating sheet, the heating cavity is located on the heating sheet, and the heating cavity is a cavity structure processed from a heat conduction block with a high thermal conductivity coefficient.

[0012] A further technical solution thereof is that: a heat dissipation device is provided in the lower space of the housing for timely dissipating the remaining heat inside the housing.

[0013] A further technical solution thereof is that: a plurality of test tube holes respectively matching each heating cavity are provided on the upper cover of the housing, and the cryopreservation tube passes through the test tube hole and is placed in the corresponding heating cavity.

[0014] The beneficial technical effects of the present invention are:

[0015] Precise temperature control: This structure controls the heating module through the main control unit to achieve timing control and temperature control for cell resuscitation; and realizes precise temperature control with the cooperation of the heat insulation of the heat insulation device.

[0016] High efficiency: Multiple independent resuscitation devices arranged at intervals can achieve simultaneous processing of multiple samples, meeting the requirements of high-throughput experiments.

[0017] Good stability: Through the temperature detection module on the peripheral side of the heating cavity, the samples in each heating cavity are monitored in real time; the temperature detection module is electrically connected to the main control unit to achieve a stable temperature control protection system.

[0018] Simple operation: Just put the cell cryopreservation container into the heating cavity, set the resuscitation time and temperature, and no manual intervention is required;

[0019] Avoid contamination: The cell resuscitation process is carried out in a closed heating cavity, effectively avoiding the contamination problem existing in the traditional water bath method. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a schematic diagram of the overall appearance of the present invention;

[0021] Figure 2It is a schematic diagram of the internal structure of the housing of the present invention;

[0022] Figure 3 It is a schematic cross-sectional view of the overall structure of the present invention;

[0023] Figure 4 It is a schematic diagram of the relevant structure of the mounting bracket of the present invention;

[0024] Wherein: 1. Housing; 11. Outer shell; 12. Upper cover; 121. Test tube hole; 2. Resuscitation device; 21. Heating module; 22. Heating chamber; 23. Temperature sensor; 3. Heat insulation device; 31. Top pin; 32. Magnetic snap; 33. Electromagnet; 34. Positioning sensor; 4. Mounting bracket; 41. Upper mounting plate; 411. Mounting hole; 42. Second mounting plate; 421. Air flow through hole; 43. Lower mounting plate; 5. Heat dissipation device; 6. Control panel. Detailed implementation manners

[0025] In order to be able to more clearly understand the technical means of the present invention and implement it in accordance with the content of the specification, the following combines the drawings and embodiments to further describe in detail the specific implementation manners of the present invention. The following embodiments are used to illustrate the present invention but are not used to limit the scope of the present invention.

[0026] As Figures 1 to 3 shown, the cell resuscitator of the present invention includes a housing 1, the housing 1 encloses an upper space and a lower space, and a plurality of spaced resuscitation devices 2 are provided in the upper space. The resuscitation device 2 realizes timing control and temperature control through a main control unit, including a heating module 21 and a heating chamber 22 that conducts heat through the heating module 21. A cell cryopreservation container can be placed in the heating chamber 22 to complete cell resuscitation.

[0027] Specifically, the heating chamber 22 is a cavity structure formed by processing a heat conduction block with a high thermal conductivity coefficient. In this embodiment, the heat conduction block is made of aluminum material. A temperature detection module for real-time monitoring of the temperature in the heating chamber 22 is provided on the periphery of the heating chamber. The temperature detection module can adopt a temperature sensor 23. A plurality of the temperature sensors 23 are embedded on the periphery of the heating chamber and are all electrically connected to the main control unit.

[0028] In this embodiment, the heating module 21 includes a heating sheet electrically connected to the main control unit. The heating chamber 22 is placed on the heating sheet, and the bottom of the heating chamber 22 matches the heating sheet. The heating chamber 22 is an aluminum heating chamber. The aluminum heating chamber has a high thermal conductivity coefficient, can quickly and effectively transfer heat to the cell cryopreservation container, enables the cells to be uniformly heated during the resuscitation process, reduces the temperature gradient, avoids local overheating or overcooling from damaging the cells, and is beneficial to improving the resuscitation survival rate of the cells.

[0029] In this embodiment, the cell cryopreservation container is a cryotube. The heating chamber 22 is a cylindrical cavity. The heating sheet is matched with the bottom of the heating chamber 22. The outer side wall of the cryotube is attached to the inner side wall of the heating chamber 22 to achieve effective heat conduction between the heating chamber 22 and the cryotube.

[0030] Furthermore, to better cooperate with the main control unit to achieve timing control and temperature control of the cell cryopreservation container, and to avoid temperature overshoot caused by the residual heat effect, thereby affecting the accuracy of temperature control. In this embodiment, a heat insulation device 3 is provided in the heating chamber 22. The temperature sensor 23 monitors the temperature of the heating chamber 22 in real time and transmits the signal to the main control unit. When the cell cryopreservation container is heated to the set temperature, the main control unit cuts off the heating power supply through the control circuit, and at the same time triggers the heat insulation device 3 to block the cryotube from the heat source.

[0031] In this embodiment, the heat insulation device 3 includes a top pin 31 and a locking device. One end of the top pin 31 is provided with an elastic element. The other end of the top pin 31 passes through the bottom of the heating chamber 22 and is connected to the locking device. The elastic element stores and releases energy through elastic deformation, and drives the top pin 31 to move when releasing energy. In this embodiment, the elastic element uses a spring, and the two ends of the spring are respectively in contact with the top boss of the top pin 31 and the bottom of the heating chamber 22.

[0032] The top pin 31 is made of heat-insulating material to effectively block the transfer of residual heat to the cell cryopreservation container and ensure precise temperature control.

[0033] The locking device includes a magnetic snap 32 connected to the bottom of the top pin 31 and an electromagnet 33 spaced below the magnetic snap 32. During use, the cryotube is placed in the heating chamber 22 and a downward pressure is applied to drive the magnetic snap 32 to move downward until the magnetic snap 32 is closed with the electromagnet 33. At this time, the elastic element is in a compressed state. The main control unit controls the electromagnet 33 to be energized to generate an electromagnetic force to maintain a stable attraction with the magnetic snap 32, and the top pin 31 is in a locked state. At the same time, the heating sheet starts to continuously heat the cryotube through the heating chamber 22. When the internal temperature of the heating chamber 22 reaches the preset value and the set working duration is completed, the main control unit immediately cuts off the power supply of the electromagnet 33, and the electromagnetic force also disappears. When the spring returns under the action of elastic potential energy, it drives the top pin 31 to move to eject the heated cryotube out of the heating chamber 22.

[0034] Furthermore, a positioning sensor 34 is provided on one side of the electromagnet 33 to detect whether the magnetic snap 32 reaches a specific position and feed back to the main control unit in a timely manner. In this embodiment, the positioning sensor 34 uses a groove switch. When the magnetic snap 32 moves downward and is closed with the electromagnet 33, at the same time, one end of the magnetic snap 32 is inserted into the groove switch. The main control unit controls the electromagnet 33 to be energized in a timely manner through the received electrical signal of the groove switch.

[0035] In this embodiment, an installation frame 4 is provided inside the housing 1. The installation frame 4 successively includes an upper mounting plate 41, a second mounting plate 42, and a lower mounting plate 43 from top to bottom.

[0036] A number of the resuscitation devices 2 are arranged in a matrix column on the upper mounting plate 41. A number of mounting holes 411 for the top pins 31 to pass through are provided on the upper mounting plate 41, as Figure 4 . In order to ensure the stable installation and precise positioning of the heating chamber 22, a downwardly extending mounting boss is provided at the lower end of the heating chamber 22. The mounting boss is cylindrical and integrally formed with the heating chamber 22. After the mounting boss passes through the mounting hole 411 and is installed, the outer side wall of the mounting boss is in close fit with the inner side wall of the mounting hole 411, thereby providing a solid and reliable mechanical support for the heating chamber 22 to prevent the heating chamber 22 from shaking and displacing during operation.

[0037] A number of the electromagnets 33 and the groove switches are provided on the second mounting plate 42.

[0038] A heat dissipation device 5 is provided in the lower space. When the cryopreservation tube pops out after heating, the heat dissipation device 5 is used to timely dissipate the residual heat inside the housing 1 to prevent excessive heat accumulation and ensure the stable operation of the internal components of the instrument and the continuous optimization of the overall performance. In this embodiment, the heat dissipation device 5 adopts a heat dissipation fan. The heat dissipation fan is provided on the lower mounting plate 43, and air circulation holes 421 for heat circulation are provided on the second mounting plate 42 to cooperate with the heat dissipation fan to discharge the heat.

[0039] The housing 1 includes an outer shell 11 and an upper cover 12. A ventilation port is provided on the side surface of the outer shell 11 corresponding to the heat dissipation fan. The upper cover 12 is provided with a number of test tube holes 121 respectively matching with the heating chambers 22. After passing through the test tube holes 121, the cryopreservation tubes are placed in the corresponding heating chambers 22.

[0040] A control panel 6 is further provided on the outer shell 11. The control panel 6 is electrically connected to the main control unit. Through the control panel 6, the cell resuscitation parameters of each cryopreservation tube can be understood and set.

[0041] During use, the cryopreservation tube is placed in the heating chamber 22. The resuscitation time and temperature are set through the control panel 6. The main control unit controls the heating sheet to work to heat the heating chamber 22. The temperature sensor 23 monitors the temperature inside the heating chamber 22 in real time and feeds back the temperature signal to the main control unit. The main control unit adjusts the power of the heating sheet according to the set temperature to keep the temperature inside the heating chamber 22 at the set value. When the set resuscitation time is reached, the main control unit triggers the operation of stopping heating. At the same time, the heat insulation device 3 acts, that is, the spring pushes the top pin 31 to eject the cryopreservation tube from the heating chamber 22 to timely block the heat source, ensuring the accuracy of the heating process and also facilitating the user to take it.

[0042] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. It should be noted that for those of ordinary skill in the art, several improvements and modifications can be made without departing from the technical principles of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A cell recovery instrument, characterized in that: The invention comprises a shell (1), wherein the upper space of the shell (1) is provided with a resuscitation device (2), wherein the resuscitation device (2) comprises a heating module (21) and a heating chamber (22) for heat conduction through the heating module (21), wherein a cell freezing container is placed in the heating chamber (22) and timing control and temperature control of cell resuscitation are achieved through a main control unit; and a heat insulation device (3) for blocking the heat source of the heated cell freezing container is provided in the heating chamber (22).

2. The cell recovery instrument according to claim 1, characterized in that: The heat insulation device (3) comprises a push pin (31) and a locking device. The push pin (31) is driven to move by the elastic deformation of the elastic element. The bottom of the push pin (31) passes through the bottom of the heating chamber (22) and is connected to the locking device.

3. The cell recovery instrument according to claim 2, characterized in that: The locking device comprises a magnetic buckle (32) connected to the bottom of the ejector pin (31) and an electromagnet (33) spaced below the magnetic buckle (32). When the magnetic buckle (32) moves downward to close with the electromagnet (33), the main control unit controls the electromagnet (33) to be energized and generate electromagnetic force, and the electromagnet (33) and the magnetic buckle (32) are kept firmly attracted under the action of the electromagnetic force.

4. The cell recovery instrument according to claim 3, characterized in that: A positioning sensor (34) for detecting the position of the magnetic buckle (32) is provided on one side of the electromagnet (33), and the positioning sensor (34) is electrically connected to the main control unit.

5. The cell recovery instrument according to claim 1, characterized in that: A mounting frame (4) is provided inside the housing (1), and a plurality of resuscitation devices (2) are arranged on the mounting frame (4) at intervals.

6. The cell recovery instrument according to claim 5, characterized in that: The mounting frame (4) comprises an upper mounting plate (41), the upper mounting plate (41) is provided with a plurality of mounting holes (411), and the lower end of the heating chamber (22) is provided with a mounting boss extending vertically downward, and the outer side wall of the mounting boss is tightly fitted with the inner side wall of the mounting hole (411).

7. The cell recovery instrument according to claim 1, characterized in that: A temperature detection module for real-time monitoring of the temperature in the heating chamber (22) is provided on the peripheral side of the heating chamber (22), and a plurality of the temperature detection modules are electrically connected to the main control unit.

8. The cell recovery instrument according to claim 2, characterized in that: The heating module (21) comprises a heating plate, the heating cavity (22) is located on the heating plate, and the heating cavity (22) is a cavity structure formed by processing a heat conduction block with a high thermal conductivity.

9. The cell recovery instrument according to claim 1, characterized in that: The lower space of the shell (1) is provided with a heat dissipation device (5) for dissipating the residual heat inside the shell (1) in a timely manner.

10. The cell recovery instrument according to claim 1, characterized in that: The upper cover (12) of the housing (1) is provided with a plurality of test tube holes (121) respectively matched with the heating chambers (22); the cryotubes are passed through the test tube holes (121) and then placed in the corresponding heating chambers (22).

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