Application of benzyl benzoate in repelling solenopsis invicta

Abstract

The invention discloses application of benzyl benzoate in preparation of a red imported fire ant repellent. Experiments prove that the feeding repellent rate of the red imported fire ant worker ant reaches 92.74% after the red imported fire ant worker ant is treated by benzyl benzoate with the concentration of more than 1 microliter / mL for 0.5 h; after treatment for 12 h, benzyl benzoate with the concentration of 10 microliters / mL or above still keeps a good repelling effect, and it is indicated that benzyl benzoate has lasting repelling activity on solenopsis invicta. And after being treated by benzyl benzoate with the concentration of more than 50 microliters per milliliter for 24 hours, the digging habit of the red imported fire ant worker ant can be obviously reduced. Benzyl benzoate with the concentration of 10 microliters / mL or above has a good repelling effect on solenopsis invicta and can be used as a solenopsis invicta protection agent in the future; benzyl benzoate with the concentration of more than 50 microliters per milliliter can be used for red imported fire ant soil repellents.

Description

Technical Field

[0001] The present invention belongs to the technical field of green prevention and control of Solenopsis invicta, and specifically relates to the application of benzyl benzoate in repelling Solenopsis invicta. Background Art

[0002] Solenopsis invicta Buren is listed as one of the 100 most harmful invasive species in the world, and it causes serious harm to public safety, ecological environment, agricultural and forestry production, and human health. Solenopsis invicta is extremely aggressive. When its ant nest is disturbed, it will launch a violent attack on the disturbance source, posing a great threat to the safety of humans and livestock. When Solenopsis invicta colonizes a certain area, it will prey on other animals and plants in the area indiscriminately, posing a great threat to the local ecological diversity. When a human body is stung by Solenopsis invicta, it will have a pain like being burned by fire, and about 24 hours after being stung, white blisters like burns will appear. People with mild allergies to the venom of Solenopsis invicta will experience itching and fever symptoms after being stung. A small number of people with moderate allergies to the venom of Solenopsis invicta will have reactions such as dizziness and swelling. In extremely rare cases, people with severe allergies to the venom of Solenopsis invicta will even experience shock symptoms, and in severe cases, it may even lead to death. Solenopsis invicta invades a wide range of habitats. In addition to occurring in wastelands and grasslands, it also occurs in agricultural lands, orchards, and ponds where human activities are frequent, and even in roadside green belts and park greenlands with a large number of people. In addition, Solenopsis invicta has the habit of liking warmth and electricity and will damage electrical facilities. The invasion of Solenopsis invicta has seriously affected people's work and life. Although ant-killing agents can effectively inhibit the spread and harm of Solenopsis invicta, they cannot play a role quickly. Therefore, screening substances with repellent activity against Solenopsis invicta has attracted more and more attention.

[0003] Insect repellents are substances that can interfere with the sensory organs of pests, making pests unable to recognize or lock their targets or stimulating insects to stay away from the targets, thereby protecting the targets from pest infestation. An ideal Solenopsis invicta repellent can reduce the occurrence of a large number of Solenopsis invicta safety incidents. So far, a variety of chemical components have been proven to have repellency against Solenopsis invicta. However, some ecologically sensitive areas such as nature reserves and organic agricultural operation areas, as well as public places with a large number of people, are not suitable for using existing irritating chemical repellents for prevention and control. In recent years, the concept of "green agriculture" has emerged more and more frequently, and the green prevention and control technology of Solenopsis invicta has also attracted more and more attention from researchers. More and more researchers focus on biological control directions such as natural plants and insects competing with Solenopsis invicta, in order to develop new, green, and environmentally friendly prevention and control methods. However, in China, the research on ant repellents started relatively late, and most of the existing insect repellents on the market are for sanitary pests such as mosquitoes, flies, mites, and ticks. At present, almost no repellent against Solenopsis invicta has been commercially put into use. Summary of the Invention

[0004] The object of the present invention is to overcome at least one deficiency of the prior art and provide an application of benzyl benzoate in repelling red imported fire ants.

[0005] The technical solution adopted by the present invention is as follows:

[0006] The present invention provides an application of benzyl benzoate in the preparation of a red imported fire ant repellent.

[0007] In some examples, the red imported fire ant repellent is used to repel the foraging of red imported fire ants.

[0008] In some examples, the red imported fire ant repellent is used to repel the excavation and nest building of red imported fire ants.

[0009] In some examples, the red imported fire ant repellent is used to repel the attack of red imported fire ants.

[0010] In some examples, the dosage of benzyl benzoate in the red imported fire ant repellent is not less than 50 μL / mL.

[0011] In some examples, the red imported fire ant repellent further comprises an acceptable carrier.

[0012] In some examples, the carrier is acetone.

[0013] The beneficial effects of the present invention are as follows:

[0014] After being treated with benzyl benzoate at a concentration above 1 μL / mL for 0.5 h, the feeding repellency rate of red imported fire ant workers reaches 92.74%; after being treated for 12 h, benzyl benzoate at a concentration above 10 μL / mL still maintains a good repellent effect (repellency rate 100%), indicating that benzyl benzoate has a persistent repellent activity against red imported fire ants. After being treated with benzyl benzoate at a concentration above 50 μL / mL for 24 h, the excavation habit of red imported fire ant workers can be significantly reduced. Benzyl benzoate at a concentration above 10 μL / mL has a good repellent effect on red imported fire ants and can be used as a red imported fire ant protection agent in the future; benzyl benzoate at a concentration above 50 μL / mL can be used as a red imported fire ant soil repellent. Description of the Drawings

[0015] Figure 1 It is the device used in the feeding repellency experiment in the examples.

[0016] Figure 2 It is the device used in the excavation repellency experiment in the examples.

[0017] Figure 3 It is the result graph of the feeding repellency experiment in the examples.

[0018] Figure 4 It is the result graph of the excavation repellency experiment in the examples. Detailed Embodiments

[0019] The following disclosure provides many different embodiments or examples for implementing different aspects of the present invention.

[0020] Examples

[0021] 1) Feeding repellency experiment

[0022] To determine the feeding repellency effect of benzyl benzoate on Solenopsis invicta, a self-made device 1 ( Figure 1 ) was used for testing. Two plastic boxes were taken as the living area and the feeding area respectively, which were connected by a connecting pipe. Teflon was coated on the edge of the boxes to prevent Solenopsis invicta from escaping.

[0023] Before the experiment, the worker ants of Solenopsis invicta used in the experiment (collected from Huadu District, Guangzhou City, Guangdong Province) were starved for 12 h. At the beginning of the experiment, 3 ± 0.1 g of the worker ants of Solenopsis invicta used in the experiment were weighed and placed in the living area of the device to adapt to the strange environment. Subsequently, 1 ± 0.1 g of ham sausage (Shuanghui, Henan Shuanghui Investment & Development Co., Ltd.) was placed in the center of a 7 cm filter paper, and 600 μL of the medicament was evenly dropped on the filter paper. The solvent of the medicament was acetone with a concentration ≥ 99.5%, and the solute was benzyl benzoate. The concentration gradient of benzyl benzoate in the medicament was set as 1 μL / mL, 10 μL / mL, and 100 μL / mL, and dropping acetone was used as a control. The filter paper moistened with the medicament at different concentration gradients was placed on an arc with the outlet of the feeding area end of the connecting pipe as the center of the circle at a distance of 10 cm from the outlet of the feeding area end of the connecting pipe. The position of the filter paper was adjusted so that the distance between adjacent filter papers was equal. At the beginning of the experiment, the small connecting pipe cap was opened and the time was started. The number of foraging worker ants on the filter paper was observed and recorded every 0.5 h for a total of 2 h, and finally recorded once after 12 h.

[0024] 2) Digging repellency experiment

[0025] To determine the digging repellency effect of benzyl benzoate on Solenopsis invicta, a self-made device 2 ( Figure 2 ) was used for testing. A culture dish with a diameter of 14 cm was taken, and Teflon was evenly smeared on the inner wall of the culture dish to prevent Solenopsis invicta from escaping. Four 5 mL sample bottles (purchased from Zhongrui Experimental Equipment Business Department, Haimen City) were fixed at a distance of 4 cm from the center of the circle along two perpendicular diameters at the bottom of the culture dish. A small hole (large enough for Solenopsis invicta to pass through) was drilled from the side of the culture dish to the side of the sample bottle using a small electric drill to connect the culture dish and the sample bottle.

[0026] The quartz sand (20 mesh - 40 mesh, Tianjin Huasheng Chemical Reagent Co., Ltd.) was dried to a constant weight. Subsequently, 5 mL of the reagent was poured into 50 g of quartz sand and stirred evenly for 5 min. Then, 5 mL of distilled water was added and stirred evenly for 5 min to make it fully mixed, and then left standing for 5 min for standby. Among them, the solvent of the reagent is acetone with a concentration ≥ 99.5%, and the solute is benzyl benzoate. The concentration gradient of benzyl benzoate was set as: 50 μL / mL, 100 μL / mL, 200 μL / mL, with the acetone solution as the control. Each sample bottle was filled with quartz sand treated with different reagents (50 μL / mL, 100 μL / mL, 200 μL / mL of benzyl benzoate, with the acetone solution as the control), and the weight of the quartz sand treated with the reagent added was 5 ± 0.1 g. Finally, 50 experimental red imported fire ant workers were placed in a petri dish, and after the device 2 was placed in the dark and left standing for 24 h, the sample bottle was removed, and the weight of the remaining quartz sand in each bottle was weighed and recorded.

[0027] 3) Data processing and analysis

[0028] In the feeding repellency experiment, R = [(C - T) / (C + T)] × 100%; R represents the repellency rate; C represents the number of insects staying on the control filter paper; T correspondingly represents the number of insects staying on the treated filter paper.

[0029] In the excavation repellency experiment, ΔD = Di - Dp; ΔD represents the excavation amount, Di represents the mass of the initial quartz sand, and Dp represents the weight of the weighed remaining quartz sand.

[0030] The Shapiro-Wilk test was used to perform a normal distribution test on the data of the number of red imported fire ant workers (T) in the treatment area and the excavation amount of quartz sand (ΔD). For those conforming to the normal distribution, a one-way analysis of variance was used for the homogeneity of variance test. For those with homogeneous variance, a one-way analysis of variance was used, and the Tukey test was used for multiple comparisons. For those not conforming to the normal distribution and non-homogeneous variance, the Kruskal-Wallis test was used for testing. All data analysis procedures were run on SPPSS 27.0.

[0031] 4) Conclusion analysis

[0032] Under indoor conditions, the feeding repellency activity and excavation repellency activity of different concentrations of benzyl benzoate on red imported fire ant workers were measured, and the experimental devices were respectively as Figure 1 , Figure 2 shown.

[0033] In the feeding repellency experiment (as Figure 3) At the same observation time, after 0.5 h of treatment, the number of foraging red imported fire ant workers in the treatment area was significantly lower than that in the control area (P < 0.001). The repellency rates of each treatment group were as follows: 92.74% for 1 μL / mL; both 10 μL / mL and 100 μL / mL reached 100%. After 2 h of treatment, the repellency rate of the 1 μL / mL treatment group decreased to 75.20%; the repellency rate of the 10 μL / mL treatment group decreased to 96.90%; while the repellency rate of the 100 μL / mL treatment group remained at 100%. After 12 h of treatment, it was observed that the number of foraging red imported fire ant workers increased only in the 1 μL / mL concentration area, and the repellency rate decreased to 35.60%. The 10 μL / mL and 100 μL / mL concentration areas still maintained a 100% repellency rate.

[0034] In the excavation repellency experiment (such as Figure 4 ), after 24 h, most of the quartz sand in the control group (CK) was excavated and transported out, while the amount of quartz sand excavated in the treatment groups (benzyl benzoate at 50 μL / mL, 100 μL / mL, and 200 μL / mL) was significantly lower than that in the control group (200 μL / mL, P = 0.007; 100 μL / mL, P = 0.005; 50 μL / mL, P = 0.033). There was no significant difference in the amount of excavation among the treatment groups.

[0035] The above is a further detailed description of the present invention, and it should not be regarded as a limitation to the specific implementation of the present invention. For those of ordinary skill in the technical field to which the present invention belongs, simple deductions or substitutions without departing from the concept of the present invention are within the protection scope of the present invention.

Claims

1. Application of benzyl benzoate in the preparation of red fire ant repellent.

2. The use according to claim 1, characterized in that: The red fire ant repellent is used for repelling the foraging of red fire ants.

3. The use according to claim 1, characterized in that: The red fire ant repellent is used for repelling the digging and nesting of red fire ants.

4. The use according to claim 1, characterized in that: The red fire ant repellent is used for repelling the attack of red fire ants.

5. The use according to claim 1, characterized in that: The amount of benzyl benzoate in the red fire ant repellent is not less than 50 μL / mL.

6. The use according to claim 1, characterized in that: The red fire ant repellent further comprises an acceptable carrier.

7. The use according to claim 6, characterized in that: The carrier is acetone.

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