Musk plaster for dispelling wind, resolving dampness and relieving pain and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine, and discloses a preparation method of musk paste for dispelling wind, resolving dampness and relieving pain, which comprises the following steps: (1) grinding and sieving traditional Chinese medicine raw materials to obtain powder, adding distilled water, uniformly mixing, adding compound enzyme for enzymolysis, and heating for enzyme deactivation to obtain enzymatic hydrolysate; (2) adding a liquid culture medium into the enzymatic hydrolysate, inoculating compound bacteria, and fermenting under an anaerobic condition to obtain fermentation liquor; (3) centrifugally separating the fermentation liquor, taking supernate, evaporating, concentrating, drying and crushing to obtain traditional Chinese medicine fermentation product powder; and (4) stirring and mixing the traditional Chinese medicine fermentation product powder with honey to obtain paste. The traditional Chinese medicine raw materials subjected to enzymolysis are fermented through compound bacteria composed of lactobacillus curvatus, bifidobacterium adolescentis and lactobacillus plantarum, the musk wind-dispelling dampness-resolving pain-relieving ointment prepared from the obtained traditional Chinese medicine fermentation product has a remarkable treatment effect, the preparation method is simple, and the prepared pain-relieving ointment is safe, free of irritation and free of toxic and side effects. Good clinical application values are realized.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and relates to a musk rheumatism-dispelling and pain-relieving plaster and a preparation method thereof. Background Art

[0002] With the increasing incidence of rheumatoid arthritis (RA) year by year, especially the elderly have been enduring the pain and suffering of rheumatoid-related diseases all year round, which has attracted people's attention. And how to treat RA has become a new hot topic and research object. For this chronic disease of RA, the great pain it brings to people's lives and spirits is self-evident. Traditional Chinese medicine has a unique perspective on RA. Traditional Chinese medicine links RA with the body's immunity and believes that it is caused by the decline of the human body, resulting in insufficient vital qi of the body and the accumulation of a large number of cold and painful symptoms, leading to the onset of the disease. Research has shown that the targets for treating RA include inhibiting the neovascularization of cartilage synovium, treating cytokines, immune cell surface antigens, etc. Traditional Chinese medicine has multiple effects in treating RA, with quick results, small side effects, and no dependence, and has unique advantages in treating RA. "External treatment of internal diseases" is an important therapy of traditional Chinese medicine. In recent years, with the gradual emergence of the side effects of oral medications, the research on external preparations has gradually increased. Summary of the Invention

[0003] The present invention belongs to the technical field of traditional Chinese medicine, and discloses a preparation method of a musk rheumatism-dispelling and pain-relieving plaster, including: (1) grinding traditional Chinese medicine raw materials, sieving to obtain powder, adding distilled water and mixing evenly, then adding a complex enzyme for enzymatic hydrolysis, and then heating to inactivate the enzyme to obtain an enzymatic hydrolysate; (2) adding a liquid medium to the enzymatic hydrolysate, sterilizing, inoculating a complex bacterium, and fermenting under anaerobic conditions to obtain a fermentation broth; (3) subjecting the fermentation broth to centrifugal separation, taking the supernatant, evaporating and concentrating, drying and pulverizing to obtain a traditional Chinese medicine fermentation product powder; (4) stirring and mixing the traditional Chinese medicine fermentation product powder with honey to make a plaster, thus obtaining the product. The musk rheumatism-dispelling and pain-relieving plaster prepared from the traditional Chinese medicine fermentation product obtained by fermenting the enzymatically hydrolyzed traditional Chinese medicine raw materials by a complex bacterium composed of Lactobacillus curvatus, Bifidobacterium adolescentis, and Lactobacillus plantarum has a significant therapeutic effect, and the preparation method is simple. The prepared pain-relieving plaster is safe and non-irritating, and has good clinical application value.

[0004] The object of the present invention can be achieved by the following technical solutions:

[0005] A preparation method of a musk rheumatism-dispelling and pain-relieving plaster, comprising the following steps:

[0006] (1) Grinding traditional Chinese medicine raw materials, sieving to obtain powder, adding distilled water and mixing evenly, then adding a complex enzyme for enzymatic hydrolysis, and after the enzymatic hydrolysis is completed, heating to inactivate the enzyme to obtain an enzymatic hydrolysate;

[0007] (2) Adding a liquid medium to the enzymatic hydrolysate, sterilizing, inoculating a complex bacterium, and fermenting under anaerobic conditions to obtain a fermentation broth;

[0008] (3) The fermentation broth is centrifuged and separated, and the supernatant is taken for evaporation and concentration, and after drying and pulverization, the traditional Chinese medicine fermentation product powder is obtained;

[0009] (4) The traditional Chinese medicine fermentation product powder is stirred and mixed with honey to make an ointment, and the Shexiang Qufeng Huashi Zhitong Ointment is obtained;

[0010] Among them, the composite bacteria in the step (2) are composed of Lactobacillus curvatus, Bifidobacterium adolescentis and Lactobacillus plantarum with a mass ratio of 1-1.5:1:1-3;

[0011] The traditional Chinese medicine raw materials in the step (1) include the following components in parts by weight: artificial musk 0.1-0.3 part, frankincense 10-15 parts, myrrh 10-15 parts, mint 20-25 parts, belladonna 10-15 parts, sinomenium acutum 10-15 parts and erigeron breviscapus 5-8 parts, and the mesh number of the sieving is 50-100 meshes.

[0012] Furthermore, the ratio among the powder, distilled water and composite enzyme in the step (1) is 1-5 g: 200-500 mL: 5-8 g, and the composite enzyme is composed of cellulase, pectinase and protease with a mass ratio of 2:2:1-1.5.

[0013] Furthermore, the temperature and time of enzymatic hydrolysis in the step (1) are 40-50 °C and 2-3 h respectively, and the temperature of enzyme inactivation by heating is 80-90 °C.

[0014] Furthermore, the mass ratio of the enzymatic hydrolysate to the liquid medium in the step (2) is 6-10:1-3, the addition amount of the composite bacteria is 2-4 wt% of the total mass of the system, and the temperature and time of fermentation are 37-42 °C and 36-52 h respectively.

[0015] Furthermore, the liquid medium in the step (2) is composed of honey, peanut cake powder, peptone, sodium acetate and distilled water with a mass ratio of 9-11:12-14:7-9:0.5-1:50-60.

[0016] Furthermore, the drying temperature in the step (3) is 40-50 °C.

[0017] The beneficial effects of the present invention:

[0018] The present invention ferments the enzymatically hydrolyzed traditional Chinese medicine raw materials with a compound bacterium composed of Lactobacillus curvatus, Bifidobacterium adolescentis, and Lactobacillus plantarum. Enzymatic hydrolysis helps release the active substances in traditional Chinese medicine, and during the fermentation process, there is also a good synergistic effect among probiotics. Using the compound bacterium for fermenting traditional Chinese medicine with a specific composition can effectively improve the medicinal efficacy of traditional Chinese medicine materials. The musk dispelling wind and dampness pain-relieving plaster obtained by preparing the obtained traditional Chinese medicine ferment has a significant therapeutic effect, and the preparation method is simple. The prepared pain-relieving plaster is safe and non-irritating, and has good clinical application value. Detailed implementation manners

[0019] To further elaborate on the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following, in combination with embodiments, details the specific implementation manners, structures, features, and their effects of the present invention as follows.

[0020] For the experimental methods without specific conditions noted in the following embodiments, they are usually carried out under conventional conditions or according to the conditions recommended by the manufacturer. The materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels.

[0021] The preservation number of Lactobacillus plantarum involved in the present invention is CGMCC No. 26774, the preservation number of Bifidobacterium adolescentis is CGMCC No. 1.2190; the preservation number of Lactobacillus curvatus is CCTCC NO: M 2016540, and the effective viable count of Lactobacillus plantarum, Lactobacillus curvatus, and Bifidobacterium adolescentis is all 1×10 6 CFU / mL.

[0022] Cellulase was purchased from Shanghai Yuanye Bio-Technology Co., Ltd., and the enzyme activity was 50 u / mg;

[0023] Pectinase was purchased from Shanghai Yuanye Bio-Technology Co., Ltd., and the enzyme activity was 50000 u / g;

[0024] Protease was purchased from Shanghai Yuanye Bio-Technology Co., Ltd., and the enzyme activity was 150 u / mg;

[0025] Peptone was purchased from Guangdong Mingtong Bio-Technology Co., Ltd., and the CAS number was 73049-73-7.

[0026] Example 1

[0027] A preparation method of a musk dispelling wind and dampness pain-relieving plaster includes the following steps:

[0028] (1) Grind the traditional Chinese medicine raw materials, sieve to obtain powder, add distilled water and mix evenly, then add a compound enzyme for enzymatic hydrolysis. After the enzymatic hydrolysis ends, heat up to inactivate the enzyme to obtain an enzymatic hydrolysate;

[0029] (2) Add a liquid medium to the enzymatic hydrolysate, sterilize it, inoculate with a complex bacterium, and ferment under anaerobic conditions to obtain a fermentation broth;

[0030] (3) The fermentation broth is centrifuged and separated, the supernatant is taken and evaporated and concentrated, and after drying and pulverizing, a traditional Chinese medicine fermentation product powder is obtained;

[0031] (4) Stir and mix the traditional Chinese medicine fermentation product powder with honey to make a paste, and obtain Shexiang Qufeng Huashi Zhitong Paste;

[0032] Among them, in the step (2), the complex bacterium is composed of Lactobacillus curvatus, Bifidobacterium adolescentis and Lactobacillus plantarum with a mass ratio of 1:1:1;

[0033] And the preservation number of Lactobacillus plantarum is CGMCC No. 26774, the preservation number of Bifidobacterium adolescentis is CGMCC No. 1.2190; the preservation number of Lactobacillus curvatus is CCTCC NO: M2016540;

[0034] In the step (1), the traditional Chinese medicine raw materials are in parts by weight: artificial musk 0.1 part, frankincense 10 parts, myrrh 10 parts, mint 20 parts, belladonna 10 parts, sinomenium acutum 10 parts, erigeron breviscapus 5 parts, and the mesh number of the sieving is 50 meshes.

[0035] In the step (1), the ratio between the powder, distilled water and complex enzyme is 1 g: 200 mL: 5 g, and the complex enzyme is composed of cellulase, pectinase and protease with a mass ratio of 2:2:1.

[0036] In the step (1), the temperature and time of enzymatic hydrolysis are 40 °C and 2 h respectively, and the temperature of heating to inactivate the enzyme is 80 °C.

[0037] In the step (2), the mass ratio of the enzymatic hydrolysate to the liquid medium is 6:1, the addition amount of the complex bacterium is 2 wt% of the total mass of the system, and the temperature and time of fermentation are 37 °C and 36 h respectively.

[0038] In the step (2), the liquid medium is composed of honey, peanut cake powder, peptone, sodium acetate and distilled water with a mass ratio of 9:12:7:0.5:50.

[0039] In the step (3), the drying temperature is 40 °C.

[0040] Example 2

[0041] A preparation method of Shexiang Qufeng Huashi Zhitong Paste, comprising the following steps:

[0042] (1) Grind the traditional Chinese medicine raw materials, sieve to obtain a powder, add distilled water and mix evenly, then add a complex enzyme for enzymatic hydrolysis, and after the enzymatic hydrolysis is completed, heat up to inactivate the enzyme to obtain an enzymatic hydrolysate;

[0043] (2) Add a liquid medium to the enzymatic hydrolysate, inoculate the complex bacteria after sterilization, and carry out fermentation under anaerobic conditions to obtain a fermentation broth;

[0044] (3) The fermentation broth is centrifuged and separated, the supernatant is taken and evaporated and concentrated, and after drying and pulverization, a traditional Chinese medicine fermentation product powder is obtained;

[0045] (4) Stir and mix the traditional Chinese medicine fermentation product powder with honey to make a paste, and obtain the Shexiang Qufeng Huashi Zhitong Paste;

[0046] Among them, the complex bacteria in the step (2) are composed of Lactobacillus curvatus, Bifidobacterium adolescentis and Lactobacillus plantarum with a mass ratio of 1.2:1:2;

[0047] And the preservation number of Lactobacillus plantarum is CGMCC No. 26774, the preservation number of Bifidobacterium adolescentis is CGMCC No. 1.2190; the preservation number of Lactobacillus curvatus is CCTCC NO: M2016540;

[0048] The traditional Chinese medicine raw materials in the step (1) are in parts by weight: artificial musk 0.2 part, frankincense 12 parts, myrrh 13 parts, mint 23 parts, belladonna 13 parts, sinomenium acutum 12 parts, erigeron breviscapus 6 parts, and the mesh number of the sieving is 80 meshes.

[0049] The ratio among the powder, distilled water and complex enzyme in the step (1) is 3 g: 350 mL: 6.5 g, and the complex enzyme is composed of cellulase, pectinase and protease with a mass ratio of 2:2:1.2.

[0050] The temperature and time of enzymatic hydrolysis in the step (1) are 45 °C and 2.5 h respectively, and the temperature of enzyme deactivation by heating is 85 °C.

[0051] The mass ratio of the enzymatic hydrolysate to the liquid medium in the step (2) is 8:2, the addition amount of the complex bacteria is 3 wt% of the total mass of the system, and the temperature and time of the fermentation are 40 °C and 46 h respectively.

[0052] The liquid medium in the step (2) is composed of honey, peanut cake powder, peptone, sodium acetate and distilled water with a mass ratio of 10:13:8:0.8:55.

[0053] The drying temperature in the step (3) is 45 °C.

[0054] Example 3

[0055] A preparation method of Shexiang Qufeng Huashi Zhitong Paste, comprising the following steps:

[0056] (1) Grind the traditional Chinese medicine raw materials, sieve to obtain powder, add distilled water and mix evenly, then add complex enzyme for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, raise the temperature to inactivate the enzyme to obtain an enzymatic hydrolysate;

[0057] (2) Add a liquid medium to the enzymatic hydrolysate, sterilize it, inoculate with complex bacteria and ferment under anaerobic conditions to obtain a fermentation broth;

[0058] (3) The fermentation broth is centrifuged and separated, and the supernatant is taken for evaporation and concentration, dried and pulverized to obtain the powder of the traditional Chinese medicine fermentation product;

[0059] (4) Stir and mix the powder of the traditional Chinese medicine fermentation product with honey to make an ointment, and obtain Shexiang Qufeng Huashi Zhitong Ointment;

[0060] Among them, in the step (2), the complex bacteria are composed of Lactobacillus curvatus, Bifidobacterium adolescentis and Lactobacillus plantarum with a mass ratio of 1.5:1:3;

[0061] And the preservation number of Lactobacillus plantarum is CGMCC No.26774, the preservation number of Bifidobacterium adolescentis is CGMCCNo.1.2190; the preservation number of Lactobacillus curvatus is CCTCC NO: M2016540;

[0062] In the step (1), the traditional Chinese medicine raw materials are in parts by weight: artificial musk 0.3 part, frankincense 15 parts, myrrh 15 parts, mint 25 parts, belladonna 15 parts, sinomenium acutum 15 parts, erigeron breviscapus 8 parts, and the mesh number of the sieving is 100 meshes.

[0063] In the step (1), the ratio among the powder, distilled water and complex enzyme is 5g:500mL:8g, and the complex enzyme is composed of cellulase, pectinase and protease with a mass ratio of 2:2:1.5.

[0064] In the step (1), the temperature and time of the enzymatic hydrolysis are 50°C and 3h respectively, and the temperature of the enzyme inactivation by heating is 90°C.

[0065] In the step (2), the mass ratio of the enzymatic hydrolysate to the liquid medium is 10:3, the addition amount of the complex bacteria is 4wt% of the total mass of the system, and the temperature and time of the fermentation are 42°C and 52h respectively.

[0066] In the step (2), the liquid medium is composed of honey, peanut cake powder, peptone, sodium acetate and distilled water with a mass ratio of 11:14:9:1:60.

[0067] In the step (3), the drying temperature is 50°C.

[0068] Comparative Example 1

[0069] Compared with Example 2, the only difference in Comparative Example 1 is that Lactobacillus curvatus is not contained in the compound bacteria, and the reduced mass is proportionally distributed to the masses of Bifidobacterium adolescentis and Lactobacillus plantarum. Other conditions are the same as those in Example 2.

[0070] Comparative Example 2

[0071] Compared with Example 2, the only difference in Comparative Example 2 is that Bifidobacterium adolescentis is not contained in the compound bacteria, and the reduced mass is proportionally distributed to the masses of Lactobacillus curvatus and Lactobacillus plantarum. Other conditions are the same as those in Example 2.

[0072] Comparative Example 3

[0073] Compared with Example 2, the only difference in Comparative Example 3 is that Lactobacillus plantarum is not contained in the compound bacteria, and the reduced mass is proportionally distributed to the masses of Lactobacillus curvatus and Bifidobacterium adolescentis. Other conditions are the same as those in Example 2.

[0074] Comparative Example 4

[0075] Compared with Example 2, the only difference is that the mass ratio of Lactobacillus curvatus, Bifidobacterium adolescentis and Lactobacillus plantarum is 2:1:4. Other conditions are the same as those in Example 2.

[0076] Comparative Example 5

[0077] Compared with Example 2, the only difference is that the mass ratio of Lactobacillus curvatus, Bifidobacterium adolescentis and Lactobacillus plantarum is 0.5:1:0.5. Other conditions are the same as those in Example 2.

[0078] Comparative Example 6

[0079] Based on Example 2, the only difference is that Lactobacillus plantarum with the preservation number of CCTCC M2010150 is used to replace Lactobacillus plantarum with the preservation number of CGMCC No.26774. Other conditions are the same as those in Example 2.

[0080] Performance Test

[0081] 1) Animal Toxicity Test:

[0082] Take the Shexiang Qufeng Huashi Zhitong Plasters prepared in Examples 1 - 3 and Comparative Examples 1 - 6 as samples, and use vaseline as the control group. Select 100 New Zealand white rabbits with a body weight of 2 kg, randomly divide them into 10 groups with 10 rabbits in each group. Shave the hair of the New Zealand white rabbits with an area of about 150 cm 2 , and apply the sample to the depilated area with an application amount of 5 g, then bandage and fix it. Administer the drug once every 12 hours for a total of 7 days, and observe the local and systemic reactions of the animals. The test results are shown in Table 1;

[0083] Skin irritation test: 100 skin-sensitive test subjects were selected and randomly divided into 10 groups of 10 people each. After applying the sample and petrolatum to the skin for 24 hours, observe whether erythema or edema appears, and evaluate the irritation intensity of the pain-relieving plaster prepared in this application on the skin (see Tables 2 and 3 for the evaluation criteria). The test results are shown in Table 4;

[0084] 2) Evaluation of the treatment effect of the pain-relieving plaster

[0085] Subjects: 180 patients with rheumatism, half male and half female, aged between 50 and 70 years old, were randomly divided into 9 groups of 20 people each. There were no significant differences in gender, age, and condition among the patients in each group. The sample was applied to the affected area once a day, with 1 month as a course of treatment, for a total of 2 courses. After the end of the course of treatment, the curative effect was statistically analyzed. The test results are shown in Table 5;

[0086] Table 1 Test results of animal toxicity testing

[0087]

[0088]

[0089] It can be seen from Table 1 that no local or systemic toxic reactions occurred in Examples 1-3, the control group, and Comparative Examples 1-6. There were no abnormal reactions in the diet, urination and defecation, eyes and mucous membranes, respiration, and limbs of the animals in each group, and there was no significant difference in the body weight growth rate.

[0090] Table 2 Skin irritation reaction and intensity scoring criteria

[0091]

[0092] Table 3 Skin irritation intensity scoring criteria

[0093] Average score Evaluation 0-0.49 No irritation 0.5-2.99 Mild irritation 3.0-5.99 Moderate irritation 6.0-8.0 Intense irritation

[0094] Table 4 Skin irritation test results

[0095] Specimen Total integral Average integral value Evaluation Example 1 2 0.2 No irritation Example 2 2 0.2 No irritation Example 3 2 0.2 No irritation Comparative example 1 4 0.4 No irritation Comparative example 2 3 0.3 No irritation Comparative example 3 2 0.2 No irritation Comparative example 4 3 0.3 No irritation Comparative example 5 2 0.2 No irritation Comparative example 6 2 0.2 No irritation Control group 1 0.1 No irritation

[0096] It can be seen from Table 4 that the examples, the control group, and the comparative examples do not cause erythema or edema on the skin of sensitive test subjects and have no irritation to the skin.

[0097] Table 5 Evaluation of the treatment effect of the pain-relieving plaster

[0098]

[0099]

[0100] Note: Marked effect (the joint function is basically restored, joint pain is significantly relieved, morning stiffness disappears, and normal work and labor can be participated in), effective (the main joint functions are basically restored, joint pain is somewhat reduced, the morning stiffness time is significantly shortened, and work and labor ability is partially restored), ineffective (the clinical symptoms have no obvious difference from those before applying the ointment, or even worsen).

[0101] Total curative effect - Marked effect (at least two of the first three items of the patient are evaluated as having a marked effect), Total curative effect - Effective (at least two of the first three items of the patient are evaluated as effective), Total curative effect - Ineffective (at least two of the first three items of the patient are evaluated as ineffective)

[0102] It can be concluded from the analysis in Table 5 that the Shexiang Qufeng Huashi Zhitong Ointment prepared in the examples of the present application has a significant curative effect on joint pain, limited flexion and extension, and morning stiffness. However, the number of people with significant effects in Comparative Examples 1-6 decreased significantly, and the number of ineffective people increased. Specifically: 1) Comparative Examples 1-3 lacked one of Lactobacillus curvatus, Bifidobacterium adolescentis, and Lactobacillus plantarum in the complex bacteria respectively. The interaction between strains during fermentation directly affects the active ingredients in the final product. Compared with the examples, the curative effect of the ointment prepared in the comparative examples was significantly weakened; 2) In Comparative Examples 4-5, the proportions of Lactobacillus curvatus, Bifidobacterium adolescentis, and Lactobacillus plantarum exceeded the range of the examples. Experiments showed that the above changes also had a greater impact on the release of active ingredients in traditional Chinese medicine raw materials; 3) In Comparative Example 6, the specific Lactobacillus plantarum strain was replaced. It can be seen that different specific strains will also have a certain impact on the effect of the ointment in dispelling wind, removing dampness, and relieving pain.

[0103] The above are only preferred embodiments of the present invention, and do not impose any form of limitation on the present invention. Although the present invention has been disclosed above with preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent embodiments by using the above-disclosed technical content within the scope of the technical solution of the present invention. However, as long as it does not depart from the content of the technical solution of the present invention, any brief modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A method for preparing a musk wind-clearing, dampness-removing and analgesic ointment, characterized in that: The following steps are involved: (1) grinding the Chinese medicine raw material, sieving to obtain powder, adding distilled water to mix, then adding a composite enzyme to perform enzymolysis, heating to inactivate the enzyme after the enzymolysis is completed, and obtaining an enzymolysis solution; (2) adding liquid culture medium to the enzymatic hydrolyzate, inoculating composite bacteria after sterilization, and fermenting under anaerobic conditions to obtain a fermentation liquid; (3) The fermentation liquid is centrifuged, the supernatant is evaporated and concentrated, and then dried and crushed to obtain a powder of the fermentation product of traditional Chinese medicine; (4) mixing the powder of the Chinese medicine fermentation product with honey to prepare an ointment, thereby obtaining a musk wind-clearing, dampness-removing and analgesic ointment; Wherein, in step (2), the composite bacteria are composed of Lactobacillus curvatum, Bifidobacterium adolescentis and Lactobacillus plantarum in a mass ratio of 1-1.5:1:1-3; The Chinese medicinal raw materials in step (1) are calculated by weight as follows: 0.1-0.3 parts of artificial musk, 10-15 parts of frankincense, 10-15 parts of myrrh, 20-25 parts of mint, 10-15 parts of belladonna, 10-15 parts of Sinomenium sinensis and 5-8 parts of Erigeron breviscapus, and the mesh number of the sieve is 50-100 mesh.

2. The method for preparing the musk wind-clearing, dampness-removing and analgesic ointment according to claim 1, characterized in that: In the step (1), the ratio of powder, distilled water and complex enzyme is 1-5g:200-500mL:5-8g, wherein the complex enzyme is composed of cellulase, pectinase and protease in a mass ratio of 2:2:1-1.

5.

3. The method for preparing the musk wind-clearing, dampness-removing and analgesic ointment according to claim 1, characterized in that: The temperature and time of enzymatic hydrolysis in step (1) are 40-50° C. and 2-3 h, respectively.

4. A method for preparing a musk wind-clearing, dampness-removing and analgesic ointment according to any one of claims 1 to 3, characterized in that: In the step (2), the mass ratio of the enzymatic hydrolysate to the liquid culture medium is 6-10:1-3, the amount of the composite bacteria added is 2-4wt% of the total mass of the system, and the fermentation temperature and time are 37-42°C and 36-52h, respectively.

5. The method for preparing the musk wind-clearing, dampness-removing and analgesic ointment according to claim 1 or 2, characterized in that: In the step (2), the liquid culture medium is composed of honey, peanut powder, peptone, sodium acetate and distilled water in a mass ratio of 9-11:12-14:7-9:0.5-1:50-60.

6. The method for preparing the musk wind-clearing, dampness-removing and analgesic ointment according to claim 1, characterized in that: The drying temperature in step (3) is 40-50°C.

7. The method for preparing the musk wind-clearing, dampness-removing and analgesic ointment according to claim 1, characterized in that: The temperature for heating and inactivating the enzyme in step (1) is 80-90°C.

8. Musk wind-clearing, dampness-removing and analgesic ointment prepared according to the preparation method according to any one of claims 1 to 7.

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