Acaricide and application thereof

Abstract

The invention belongs to the technical field of pesticide mite killing, and discloses an acaricide and application thereof.The acaricide comprises an active component A and an active component B. The active component A is a compound shown in the formula I: # imgabs0, the active component B is any one of etoxazole, lufenuron or flufenoxystrobin, and the mass ratio of the active component A to the active component B is 1: 50-45: 1. The acaricide disclosed by the invention has an excellent prevention and treatment effect on common harmful mites on crops and garden plants, and can effectively reduce the use amount of pesticides and reduce the harm to the environment.

Description

[0001] This divisional application of the present invention has an application number of 202311368221.X, a filing date of October 23, 2023, and an invention title of "A miticide and its application". Technical Field

[0002] The present invention belongs to the technical field of pesticide miticides and discloses a miticide and its application. Background Art

[0003] Etoxazole, CAS No.: 153233-91-1, chemical name: 4-(4-tert-butyl-2-ethoxyphenyl)-2-(2,6-difluorophenyl)-4,5-dihydro-1,3-oxazole, is an oxazole miticide belonging to diphenyl oxazoline derivatives. It mainly controls the normal molting process of mites and has ovicidal activity, so it can effectively control the entire juvenile stage of mites and also cause female adult mites to be infertile. Its control targets mainly include spider mites on apples and citrus, and it also has excellent control effects on mite species such as spider mites, Eotetranychus spp., Panonychus spp., Tetranychus urticae, and Tetranychus cinnabarinus on crops such as cotton, flowers, and vegetables.

[0004] Lufenuron, CAS No.: 103055-07-8, chemical name: N-[[2,5-dichloro-4-(1,1,2,3,3,3-hexafluoropropoxy)phenyl]carbamoyl]-2,6-difluorobenzamide, belongs to a chitin synthesis inhibitor. Its mechanism of action is to inhibit the enzyme that catalyzes chitin polymerization in pest mites / insects and interfere with the normal growth and development of insects.

[0005] Fluacrypyrim, CAS No.: 918162-02-4, chemical name: (2E)-2-(2-{[2-chloro-4-(trifluoromethyl)phenoxy]methyl}phenyl)-3-methoxyacrylate methyl ester, is a high-efficiency methoxyacrylate fungicide and miticide. It can be used for the control of diseases caused by Deuteromycetes, Ascomycetes, Basidiomycetes, and Zygomycetes such as wheat rust, powdery mildew, cucumber powdery mildew, anthracnose, scab, maize leaf spot, and rice sheath blight. It also has high activity against mite species such as Tetranychus cinnabarinus, such as spider mites on apples and citrus.

[0006] Mites are a type of phytophagous arthropods, belonging to the phylum Arthropoda, class Arachnida, order Acarina. They have few individuals, a wide range of activities, reproduce rapidly under high temperature and drought, have a short generation cycle, and strong drug resistance. Agricultural pest mites are one of the biological groups recognized worldwide as difficult to control. In China, there are more than 40 species of agricultural pest mites, which have extremely strong destructive ability to economic crops such as fruit trees, vegetables, and flowers, seriously affecting the normal physiological functions of crop leaves and even reducing yields. At present, the prevention and control of pest mites mainly adopt chemical control methods, and acaricides are the main means of modern agricultural control of pest mites. In agricultural production, the control effect of various acaricides on pest mites has been decreasing year by year. The applicant has found through research that the compounding of sulfiflumin and etoxazole, lufenuron, or flufenerim in a suitable mass ratio has an obvious synergistic effect on pest mites, effectively improving the control effect of field pest mites, being safe for plants, reducing the dosage of pesticides, and reducing environmental pollution. Summary of the Invention

[0007] Based on the above content, the object of the present invention is to provide an acaricide, and the acaricide has a significant synergistic effect on pest mites on agricultural and garden plants, can effectively reduce the usage amount of pesticides, and reduce the harm to the environment.

[0008] In order to achieve the above object, the present invention adopts the following technical solution: An acaricide, the acaricide contains active ingredient A and active ingredient B, and the active ingredient A is a compound of formula I: The active ingredient B is any one of etoxazole, lufenuron, or flufenerim;

[0009] Further, the mass ratio of active ingredient A to active ingredient B in the acaricide is 1:50 to 45:1.

[0010] Further, the mass ratio of active ingredient A to active ingredient B in the acaricide is 1:45 to 35:1;

[0011] Further, the active ingredient B is etoxazole, and the mass ratio of active ingredient A to active ingredient B is 1:45 to 35:1;

[0012] The active ingredient B is lufenuron, and the mass ratio of active ingredient A to active ingredient B is 1:35 to 35:1;

[0013] The active ingredient B is flufenerim, and the mass ratio of active ingredient A to active ingredient B is 1:30 to 15:1;

[0014] Further, based on the total weight of the acaricide being 100%, the total weight of active ingredient A and active ingredient B accounts for 0.1% to 80% of the total weight of the acaricidal composition;

[0015] Furthermore, the acaricide, in addition to the active ingredient, also contains auxiliary ingredients permitted in pesticides, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers.

[0016] Furthermore, the acaricide can be prepared into a pesticide-acceptable formulation dosage form, and the formulation dosage form is a solid preparation or a liquid preparation.

[0017] Furthermore, the solid preparation is a granule, a strip, a wettable powder, an oil-dispersible powder, a milk powder, a water-dispersible granule, a milk granule, a water-dispersible tablet, a soluble powder, a soluble tablet or a soluble granule, and the liquid preparation is a soluble solution, a sol, an oil, a film-forming oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, a water emulsion, an oil emulsion, a microemulsion, a fat agent, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension or a suspoemulsion;

[0018] Furthermore, the solid preparation is a wettable powder or a water-dispersible granule, and the liquid preparation is an emulsifiable concentrate, a water suspension or a water-dispersible granule;

[0019] The present invention also discloses the application of the acaricide as described above in controlling pest mites on crops and garden plants.

[0020] Furthermore, the pest mites on plants are Tetranychus cinnabarinus, Tetranychus urticae or Panonychus citri.

[0021] The present invention has the following advantages:

[0022] The acaricide of the present invention has a significant synergistic effect on common pest mites on agricultural and garden plants, especially has a good control effect on Tetranychus cinnabarinus, Tetranychus urticae and Panonychus citri, can slow down the development of pest mite resistance, reduce the amount of pesticide used, is safe for plants and friendly to the environment. Specific Embodiments

[0023] In order to make the technical solutions of the present invention clearer and more understandable, the technical solutions of the present invention are explained and illustrated in combination with specific preparation examples and examples.

[0024] Formulation Preparation Example:

[0025] Preparation Example 1: 27% sulfiflumin·etoxazole suspension (1:8)

[0026] Formulation composition: 3% sulfiflumin, 24% etoxazole, 1% fatty alcohol polyoxyethylene ether, 5% styrylphenol polyoxyethylene ether phosphate, 1% sodium lignosulfonate, 0.25% xanthan gum, 5% ethylene glycol, 0.1% sodium benzoate, 0.5% silicone oil, and deionized water is added to make up the balance;

[0027] Preparation method: According to the formula ratio, the active ingredient, surfactant and other functional auxiliaries are sequentially placed in a reaction kettle, mixed evenly with water, subjected to high-speed shearing and wet grinding, and finally homogenized and filtered to obtain the suspension product.

[0028] Preparation Example 2: 42% sulfiflumin·etoxazole water dispersible granule (5:1)

[0029] Formulation composition: 35% sulfiflumin, 7% etoxazole, 8% sodium lignosulfonate, 2% Nekal BX, 8% sodium polycarboxylate, 4% ammonium sulfate, kaolin to make up the balance;

[0030] Preparation method: According to the formula ratio of the example, the active ingredient is added to the carrier, and a surfactant and other functional auxiliaries are added thereto, mixed, 10 - 25% of water is added after air-flow pulverization, and then kneaded, granulated, dried and sieved to obtain the water dispersible granule product; or the pulverized powder is sprayed with water, granulated and dried in a fluidized bed granulator, and then sieved to obtain the product.

[0031] Preparation Example 3: 16% sulfiflumin·etoxazole emulsifiable concentrate (1:3)

[0032] Formulation composition: 4% sulfiflumin, 12% etoxazole, 15% EO / PO block copolymer, 12% acetophenone, 10% propylene glycol methyl ether, 1% calcium dodecylbenzenesulfonate, xylene to make up the balance;

[0033] Preparation method: According to the formula ratio of the example, the metered active ingredient, solvent and cosolvent are added to a blending kettle and stirred to dissolve, then an emulsifier is added, and the balance is made up with the remaining solvent, and stirred evenly in a stirring kettle, and filtered to obtain the emulsifiable concentrate required by the present invention.

[0034] Preparation Example 4: 12% sulfiflumin·lufenuron emulsifiable concentrate (1:2)

[0035] Formulation composition: 4% sulfiflumin, 8% lufenuron, 15% N-methylpyrrolidone, 14% styrylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 10% DMF, methyl oleate to make up the balance;

[0036] Preparation method: The same as Preparation Example 3.

[0037] Preparation Example 5: 21% sulfiflumin·lufenuron suspension (6:1)

[0038] Formulation composition: 18% sulfiflumin, 3% lufenuron, 2% Guerbet alcohol polyoxyethylene ether, 3% alkylaryl polyoxyethylene polyoxypropylene ether, 2% triphenylvinylphenol ethoxylate phosphate, 1% sodium polycarboxylate, 1.5% magnesium aluminum silicate, 0.2% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance;

[0039] Preparation method: same as Preparation Example 1.

[0040] Preparation Example 6: 30% sulfiflumin·lufenuron water dispersible granule (3:2)

[0041] Formulation composition: 18% sulfiflumin, 12% lufenuron, 10% dispersant NNO, 2% sodium dodecyl sulfate, 6% sodium lignosulfonate, 30% starch, light calcium carbonate to make up the balance;

[0042] Preparation method: same as Preparation Example 2.

[0043] Preparation Example 7: 27% sulfiflumin·fludioxonil suspension concentrate (4:5)

[0044] Formulation composition: 12% sulfiflumin, 15% fludioxonil, 2% isomeric tridecanol polyoxyethylene ether, 4% triphenylvinylphenol ethoxylate phosphate, 1% lignosulfonate, 3% alkylaryl polyoxyethylene polyoxypropylene ether, 0.25% xanthan gum, 5% glycerol, 0.1% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance;

[0045] Preparation method: same as Preparation Example 1.

[0046] Preparation Example 8: 39% sulfiflumin·fludioxonil water dispersible granule (1:12)

[0047] Formulation composition: 3% sulfiflumin, 36% fludioxonil, 8% sodium lignosulfonate, 2% Nekal BX, 8% naphthalene sulfonate formaldehyde condensate D425, 5% white sugar, kaolin to make up the balance;

[0048] Preparation method: same as Preparation Example 2.

[0049] Preparation Example 9: 16% sulfiflumin·fludioxonil emulsifiable concentrate (7:1)

[0050] Formulation composition: 14% sulfiflumin, 2% fludioxonil, 15% EO / PO block copolymer, 15% acetophenone, 10% N-octylpyrrolidone, 1% calcium dodecylbenzenesulfonate, mesitylene to make up the balance;

[0051] Preparation method: same as Preparation Example 3.

[0052] The above-prepared preparation examples all meet the quality and technical indicators required for the corresponding preparations after testing, and the prepared preparations are qualified preparations recognized in the art.

[0053] Example 1: Indoor bioactivity determination test

[0054] Test basis: The test refers to NY / T 1154.12-2008 "Guidelines for Pesticide Indoor Bioassay Tests Insecticides Part 12: Slide Immersion Method for Spider Mites".

[0055] Test targets: Tetranychus urticae Koch, Tetranychus cinnabarinus, and nymphs with consistent physiological states were selected.

[0056] Test agents: sulfiflumin technical, etoxazole technical, lufenuron technical, flufenerim technical, provided by the Group R & D Center.

[0057] Test preparation: Cut double-sided tape into 2 cm long pieces, stick one end to the slide, select healthy mites, stick their backs to the double-sided tape (do not stick to the legs, antennae, and mouthparts), 30 mites per slide, place them in a container lined with a wet sponge, cover with a lid, and place at 25 ± 1 °C. After 2 h, examine under a microscope, remove dead and injured individuals, and make up to 30 mites per slide.

[0058] Agent preparation: Dissolve the test agents in a suitable solvent, and prepare 5 series of mass concentration gradients for each single agent and each mixture ratio according to the mixing purpose and agent activity design.

[0059] Agent treatment: Immerse the slide in the liquid medicine and gently shake for 5 s, then take it out, blot the excess liquid medicine with filter paper, place it in a white porcelain dish lined with a wet sponge, and cover it with a plastic film with good light transmittance. Each treatment has 4 replicates, and a treatment without agent (including all organic solvents and emulsifiers) is set as a blank control.

[0060] Rearing and observation: Place the container with the test insects at (25 ± 1) °C and a photoperiod of L:D = (16:8) h for rearing and observation.

[0061] Test investigation: Examine under a microscope 48 h after treatment, check the death situation of the test insects, and record the total number of insects and the number of dead insects respectively.

[0062] Based on the investigation data, calculate the mortality rate of each treatment. Calculate according to the following formula:

[0063]

[0064] Where:

[0065] P—the mortality rate, in percentage (%);

[0066] K—the number of dead insects, in number;

[0067] N—the total number of insects in the treatment, in number.

[0068]

[0069] In the formula:

[0070] P1—the corrected mortality rate, in percentage (%);

[0071] P t —the mortality rate of the treatment, in percentage (%);

[0072] P0—the mortality rate of the blank control, in percentage (%).

[0073] If the mortality rate of the control < 5%, no correction is needed; if the mortality rate of the control is between 5% and 20%, correction should be made according to formula (2); if the mortality rate of the control > 20%, the test needs to be redone.

[0074] Analyze with the DPS statistical analysis system to obtain the LC 50 value and evaluate the activity of the test agent against the biological test material.

[0075] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formulas (3), (4), and (5):

[0076]

[0077] In the formula:

[0078] ATI—the measured toxicity index of the mixture;

[0079] S—the LC 50 , in milligrams per liter (mg / L);

[0080] M—the LC 50 , in milligrams per liter (mg / L).

[0081] TTI = TI A ×P A +TI B ×P B

[0082] In the formula:

[0083] TTI—the theoretical toxicity index of the mixture;

[0084] TI A——Virulence index of Agent A;

[0085] P A ——Percentage content of Agent A in the mixture, in percentage (%);

[0086] TI B ——Virulence index of Agent B;

[0087] P B ——Percentage content of Agent B in the mixture, in percentage (%).

[0088]

[0089] Where:

[0090] CTC - Co-toxicity coefficient;

[0091] ATI - Actual measured virulence index of the mixture;

[0092] TTI - Theoretical virulence index of the mixture.

[0093] When the co-toxicity coefficient CTC of the compounding is ≥ 120, it shows a synergistic effect; when CTC ≤ 80, it shows an antagonistic effect; when 80 < CTC < 120, it shows an additive effect.

[0094] The results of the indoor virulence test are shown in the following table:

[0095] Table 1 Test results of the indoor bioactivity of the mixture of sulfiflumin and etoxazole against Tetranychus urticae

[0096] Test agents Toxicity regression equation <![CDATA[LC 50 (mg / L)]]> Coefficient of co-toxicity Sulfiflumin(A) y = 5.4740 + 1.5962x 0.5047 / Etoxazole(B) y = 3.9338 + 1.5138x 5.0615 / A:B = 1:50 y = 4.0496 + 1.5161x 4.2352 101.535 A:B = 1:45 y = 4.2414 + 1.4151x 3.4358 123.146 A:B = 1:35 y = 4.2959 + 1.6452x 2.6789 151.055 A:B = 1:25 y = 4.4364 + 1.5463x 2.3144 162.327 A:B = 1:10 y = 4.6920 + 1.4960x 1.6065 173.037 A:B = 1:5 y = 4.9408 + 1.2350x 1.1167 180.955 A:B = 5:1 y = 5.8459 + 1.8599x 0.3509 169.221 A:B = 10:1 y = 5.8399 + 1.8213x 0.3458 158.961 A:B = 25:1 y = 5.6494 + 1.5785x 0.3878 134.812 A:B = 35:1 y = 5.5827 + 1.5207x 0.4138 125.096 A:B = 45:1 y = 5.5198 + 1.5875x 0.4705 109.410

[0097] From the results of the indoor biological test, it can be known that when sulfiflumin and etoxazole are compounded in a suitable mass ratio, it has excellent control effect against Tetranychus urticae. Among them, the mass ratio of sulfiflumin to etoxazole is 1:45 to 35:1, and the co-toxicity coefficient is greater than 120, showing a synergistic effect.

[0098] Table 2 Test results of the indoor bioactivity of the mixture of sulfiflumin and lufenuron against Tetranychus urticae

[0099]

[0100]

[0101] From the results of the indoor determination test, it can be known that when sulfiflumin and lufenuron are compounded, when the mass ratio of the two is 20:1 to 1:35, the co-toxicity coefficient against Tetranychus urticae is greater than 120, showing a synergistic effect.

[0102] Table 3 Results of indoor bioactivity determination test on Tetranychus urticae Koch by the mixture of sulfiflumin and fludioxonil

[0103] Test agents Toxicity regression equation <![CDATA[LC 50 (mg / L)]]> Coefficient of co-toxicity Sulfiflumin(A) y = 5.4740 + 1.5962x 0.5047 / Flufenerim(B) y = 3.8921 + 1.7595x 4.2626 / A:B = 1:45 y = 4.2510 + 1.4573x 3.2658 112.339 A:B = 1:25 y = 4.3735 + 1.5011x 2.6142 126.756 A:B = 1:15 y = 4.5345 + 1.5005x 2.0428 142.398 A:B = 1:7 y = 4.7835 + 1.4748x 1.4022 157.450 A:B = 1:3 y = 5.0596 + 1.5926x 0.9174 162.379 A:B = 1:1 y = 5.3465 + 1.4701x 0.5811 155.315 A:B = 3:1 y = 5.5544 + 1.6337x 0.4578 141.412 A:B = 7:1 y = 5.5391 + 1.4118x 0.4151 136.643 A:B = 15:1 y = 5.4453 + 1.1766x 0.4183 127.691 A:B = 25:1 y = 5.3968 + 1.2876x 0.4918 106.225 A:B = 45:1 y = 5.2517 + 0.9649x 0.5484 93.830

[0104] From the results of indoor bioassay, it can be known that when sulfiflumin is compounded with fludioxonil at a mass ratio of 1:25 to 15:1, it shows a synergistic effect on Tetranychus urticae Koch.

[0105] Table 4 Results of indoor bioactivity determination test on Tetranychus cinnabarinus Boisduval by the mixture of sulfiflumin and etoxazole

[0106] Test agents Toxicity regression equation <![CDATA[LC 50 (mg / L)]]> Coefficient of co-toxicity Sulfiflumin(A) y = 5.1145 + 1.3081x 0.8175 / Etoxazole(B) y = 3.7663 + 1.4953x 6.6842 / A:B = 1:50 y = 4.0485 + 1.4018x 4.7727 122.775 A:B = 1:45 y = 4.2043 + 1.3369x 3.9372 146.859 A:B = 1:35 y = 4.2168 + 1.3826x 3.6854 151.224 A:B = 1:25 y = 4.3367 + 1.3116x 3.2045 163.468 A:B = 1:8 y = 4.6126 + 1.3888x 1.9008 195.647 A:B = 1:5 y = 4.6697 + 1.4312x 1.7014 178.895 A:B = 5:1 y = 5.3287 + 1.4421x 0.5916 161.862 A:B = 8:1 y = 5.2649 + 1.1412x 0.5860 154.580 A:B = 25:1 y = 5.1907 + 1.1607x 0.6851 123.495 A:B = 35:1 y = 5.1921 + 1.3239x 0.7160 117.029 A:B = 45:1 y = 5.0652 + 1.0736x 0.8695 95.848

[0107] From the results of indoor bioassay, it can be known that when sulfiflumin is compounded with etoxazole at a mass ratio of 1:45 to 25:1, it shows a synergistic effect on Tetranychus cinnabarinus Boisduval.

[0108] Table 5 Results of indoor bioactivity determination test on Tetranychus cinnabarinus Boisduval by the mixture of sulfiflumin and lufenuron

[0109] Test agents Toxicity regression equation <![CDATA[LC 50 (mg / L)]]> Coefficient of co-toxicity sulfiflumin(A) y = 5.1145 + 1.3081x 0.8175 / lufenuron(B) y = 3.1179 + 1.6172x 14.5820 / A:B = 35:1 y = 5.2414 + 1.4879x 0.6883 121.969 A:B = 20:1 y = 5.3282 + 1.4803x 0.6002 142.615 A:B = 10:1 y = 5.4303 + 1.7083x 0.5599 159.714 A:B = 8:1 y = 5.3718 + 1.4601x 0.5563 164.172 A:B = 4:1 y = 5.4257 + 1.7504x 0.5712 176.427 A:B = 1:1 y = 5.0283 + 1.3805x 0.9538 162.320 A:B = 1:4 y = 4.5690 + 1.3264x 2.1132 157.996 A:B = 1:8 y = 4.1602 + 1.5130x 3.5897 141.499 A:B = 1:10 y = 4.1724 + 1.3251x 4.2127 136.780 A:B = 1:20 y = 3.8738 + 1.3203x 7.1290 113.524 A:B = 1:35 y = 3.7466 + 1.2794x 9.5425 104.116

[0110] From the results of indoor bioassay, it can be known that when sulfiflumin is compounded with lufenuron at a mass ratio of 1:10 to 35:1, it shows a synergistic effect on Tetranychus cinnabarinus Boisduval.

[0111] Table 6 Results of indoor bioactivity determination test on Tetranychus cinnabarinus Boisduval by the mixture of sulfiflumin and fludioxonil

[0112] Test agents Toxicity regression equation <![CDATA[LC 50 (mg / L)]]> Coefficient of co-toxicity sulfiflumin(A) y = 5.1145 + 1.3081x 0.8175 / fludioxonil(B) y = 3.5223 + 1.7130x 7.2884 / A:B = 1:40 y = 3.8874 + 1.5416x 5.2694 115.933 A:B = 1:30 y = 4.0599 + 1.5073x 4.2047 138.082 A:B = 1:15 y = 4.3127 + 1.3569x 3.2101 151.899 A:B = 1:7 y = 4.6180 + 1.4351x 1.8456 198.502 A:B = 1:3 y = 4.7781 + 1.4987x 1.4062 173.994 A:B = 1:1 y = 5.0109 + 1.4576x 0.9829 149.568 A:B = 3:1 y = 5.1906 + 1.4383x 0.7370 142.567 A:B = 7:1 y = 5.2521 + 1.4608x 0.6721 136.818 A:B = 15:1 y = 5.2297 + 1.5358x 0.7087 122.129 A:B = 30:1 y = 5.1192 + 1.5014x 0.8330 101.033 A:B = 40:1 y = 5.0074 + 1.2794x 0.9867 84.686

[0113] From the results of indoor bioassay, it can be known that when sulfiflumin is compounded with fludioxonil at a mass ratio of 1:30 to 15:1, it shows a synergistic effect on Tetranychus cinnabarinus Boisduval.

[0114] Example 2: Field efficacy test of acaricidal composition against Tetranychus urticae Koch

[0115] Test site: This test was carried out in Shaojiazhuang Village, Mancheng District, Baoding City, Hebei Province. The soil fertility of this test site is relatively high, and the cultivation and management conditions are good. The water and fertilizer management of all test plots is uniform and consistent, which conforms to the local scientific agricultural practice.

[0116] Test target: Tetranychus urticae Koch on strawberries.

[0117] Test crop: Strawberries.

[0118] Experimental design: A total of 8 treatments were set up in the experiment, with clear water as the blank control. Each treatment was replicated 4 times, and the area of each plot was 30 m 2 , and a randomized block arrangement was adopted, with two rows of strawberries spaced between each plot.

[0119] Experimental method: When applying the medicine, a 3WBD-20 knapsack electric sprayer was used to evenly spray the liquid medicine on both the front and back sides of the strawberry leaves. The medicine application time was March 22, 2023, and the medicine was applied only once in total.

[0120] Investigation method: The mite population base was investigated before applying the medicine, and the insect population density was investigated on the 3rd day and 10th day after applying the medicine. When investigating, a hand-held magnifying glass was used to observe and record the number of live mites on both the front and back sides of the leaves. Five-point sampling was carried out in each plot, one plant was fixed at each point, and five mite-infested leaves were fixed on each plant to investigate the mite population.

[0121] Pharmacodynamic calculation method:

[0122]

[0123] Results and analysis:

[0124] Table 7 Results of the field pharmacodynamic test of the acaricidal composition against Tetranychus urticae

[0125]

[0126] As can be seen from the field pharmacodynamic test in Table 7, the control effects of the 27% sulfiflumin·etoxazole suspension concentrate (1:8), 21% sulfiflumin·lufenuron suspension concentrate (6:1), and 16% sulfiflumin·flufenerim emulsifiable concentrate (7:1) against Tetranychus urticae were 85.53%, 81.00%, and 80.15% on the 3rd day after application, and 93.27%, 92.52%, and 89.44% on the 14th day after application, respectively.

[0127] Example 3: Field pharmacodynamic test of the acaricidal composition against Tetranychus cinnabarinus

[0128] Test site: The test was carried out in a vegetable greenhouse in Shouguang City, Weifang City, Shandong Province. The soil layer of the test site was deep, and the soil fertility was medium to high. All test plots conformed to local conventional agricultural practices.

[0129] Test target: Tetranychus cinnabarinus.

[0130] Test crop: Eggplant.

[0131] Experimental design: The area of each plot in the test field was 20 m 2, all experimental plots were arranged in a randomized complete block design, and each treatment was replicated 4 times. The experiment was carried out at the initial stage of the occurrence of Tetranychus cinnabarinus. When applying the medicine, each chemical treatment was diluted to a certain concentration, and a Gongnong-16 type sprayer was used to spray the entire eggplant plant, ensuring that both the front and back sides of each leaf were evenly covered with the medicine.

[0132] Investigation method: Fix 5 eggplant plants in each plot, and investigate 3 leaves on each eggplant plant. The base number of Tetranychus cinnabarinus was investigated before applying the medicine, and the number of surviving mites was investigated 3 days, 7 days, and 15 days after applying the medicine. The mite population reduction rate and control effect were calculated.

[0133] Calculation method of drug efficacy:

[0134]

[0135] Results and analysis of field efficacy trials:

[0136] Table 8 Results of field efficacy trials of acaricidal compositions against Tetranychus cinnabarinus

[0137]

[0138] It can be seen from the results of the field efficacy trials that the combination of sulfiflumin with etoxazole, lufenuron, or flufenerim has good control effects against Tetranychus cinnabarinus. The 42% sulfiflumin·etoxazole water dispersible granules (5:1), 30% sulfiflumin·lufenuron water dispersible granules (3:2), and 27% sulfiflumin·flufenerim suspension concentrate (4:5) showed good quick-acting effects against Tetranychus cinnabarinus 3 days after applying the medicine. 15 days after applying the medicine, the control effects of the 42% sulfiflumin·etoxazole water dispersible granules (5:1), 30% sulfiflumin·lufenuron water dispersible granules (3:2), and 27% sulfiflumin·flufenerim suspension concentrate (4:5) against Tetranychus cinnabarinus were 93.59%, 95.64%, and 92.85%, respectively, and the long-lasting period was relatively long.

[0139] Example 4: Field efficacy trial of acaricidal composition against Panonychus citri

[0140] Test site: A citrus orchard in Qingcao Village, Qingjiang Town, Zixing City, Hunan Province. The water and fertilizer conditions and cultivation management in this area are relatively consistent. The test crop is Dongjiang tangerine, and its growth is normal.

[0141] Experimental Design: In this experiment, 7 chemical treatments and 1 clear water control were set. The chemical treatments were as follows: 16% sulfiflumin·etoxazole EC (1:3), 16% sulfiflumin·fluacrypyrim EC (7:1), 12% sulfiflumin·lufenuron EC (1:2), 20% etoxazole SC, 22% lufenuron EC, 21% fluacrypyrim SC, 20% sulfiflumin WG.

[0142] Experimental Plot Setting: Each treatment had 3 trees, with 4 replicates arranged randomly. Protection rows were set around the experimental plots. During the experiment, no chemicals or clear water were applied to the protection rows, and they were managed conventionally.

[0143] Application Time: On May 13, 2023, the weather was cloudy with gentle breeze during application. Uniform foliar spraying was used. When spraying, the front and back sides of the leaves were evenly covered with the liquid medicine until it just started to drip.

[0144] Investigation Method: The mite population was investigated before application, and the number of live mites was investigated 3 days, 8 days, and 14 days after application. In each plot, young shoots were marked at five positions: east, south, west, north, and middle of each tree. The number of active mites on 25 leaves of each tree was investigated, and a hand-held magnifying glass was used to directly observe the leaf surface to count the number of mites.

[0145] Calculation Method of Control Efficacy:

[0146]

[0147] Results and Analysis of Field Control Efficacy Test:

[0148] Table 9 Results of Field Control Efficacy Test of Mite Control Composition against Panonychus citri

[0149]

[0150] As can be seen from Table 9, the mite control composition of the present invention has good control efficacy against Panonychus citri, and its quick-acting property and long-lasting period are significantly better than those of the single-agent control.

[0151] Although the present invention has been described in detail with general descriptions and specific embodiments above, based on the present invention, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of the present invention claimed.

Claims

1. A miticide, characterized in that, The acaricide described above contains active ingredient A and active ingredient B. The active ingredient A is a compound of formula I: The active ingredient B is lufenuron.

2. The acaricide according to claim 1, characterized in that, In the acaricide described, the mass ratio of active ingredient A to active ingredient B is 1:50 to 45:1; Preferably, the mass ratio of active ingredient A to active ingredient B is 1:45 to 35:

1.

3. The acaricide according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:35 to 20:1; Preferably, the mass ratio of active ingredient A to active ingredient B is 1:10 to 35:

1.

4. The acaricide according to claim 1, characterized in that, Based on the total weight of the acaricide being 100 wt%, the total weight of active ingredient A and active ingredient B accounts for 0.1 wt% to 80 wt% of the total weight of the bactericidal composition.

5. The acaricide according to claim 1, characterized in that, In addition to the active ingredients, the acaricide described further contains auxiliary ingredients permitted in pesticides, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers.

6. The acaricide according to claim 1, characterized in that, The acaricide described can be prepared into a pesticide-acceptable formulation dosage form, and the formulation dosage form is a solid preparation or a liquid preparation.

7. The acaricide according to claim 6, characterized in that, The solid preparation is a granule, wettable powder, oil-dispersible powder, milk powder, water-dispersible granule, milk granule, water-dispersible tablet, soluble powder, soluble tablet or soluble granule, and the liquid preparation is a soluble solution, sol, oil, film-forming oil, emulsifiable concentrate, latex, dispersible liquid, paste, water emulsion, oil emulsion, microemulsion, fat agent, suspension, microcapsule suspension, oil suspension, dispersible oil suspension or suspension emulsion.

8. The acaricide according to claim 7, characterized in that, The solid preparation is a wettable powder or water-dispersible granule, and the liquid preparation is an emulsifiable concentrate, water emulsion, microemulsion, suspension, dispersible oil suspension.

9. The application of the acaricide according to any one of claims 1-8 in controlling pest mites on crops and garden plants.

10. The use according to claim 9, characterized in that, The pest mites on plants are Tetranychus cinnabarinus, Tetranychus urticae, Panonychus citri.

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