Fermented traditional Chinese medicine feed additive as well as preparation method and application thereof

Abstract

The invention discloses a fermented traditional Chinese medicine feed additive as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese veterinary medicines. The additive is prepared by solid state fermentation and extraction of a traditional Chinese medicine composition and a complex microbial inoculant, the traditional Chinese medicine composition comprises poria cocos, astragalus membranaceus, codonopsis pilosula, pericarpium citri reticulatae and radix puerariae, and the complex microbial inoculant comprises bacillus subtilis, lactobacillus plantarum and aspergillus niger. Through a fermentation and extraction combined process, the effective components in the traditional Chinese medicines are higher in extraction rate and easier to absorb, the animal intestinal flora balance can be remarkably regulated, the immunity is enhanced, the growth performance is promoted, and the prospect is wide.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese veterinary medicines, and specifically relates to a fermented traditional Chinese medicine feed additive and a preparation method and application thereof. Background Art

[0002] With the intensive development of animal husbandry, the long-term addition of antibiotics in feed has led to serious bacterial resistance problems, and drug residues pose a potential threat to food safety and human health. In recent years, feed "antibiotic ban" policies have been introduced around the world. Announcement No. 194 of the Ministry of Agriculture and Rural Affairs of my country also clearly stipulates that the addition of growth-promoting antibiotics to feed will be completely banned from 2020. In this context, the development of safe and efficient alternatives to antibiotics has become a key issue that needs to be urgently addressed in the animal husbandry industry. Although alternative antibiotic products such as plant extracts, enzyme preparations, and microecological preparations have certain effects, they are still limited in stability and functionality, and it is difficult to completely replace the growth-promoting and disease-preventing effects of antibiotics.

[0003] The use of probiotics to ferment Chinese herbal medicines to prepare feed additives is a current research hotspot. Compared with traditional Chinese medicine additives, the fermentation process can decompose plant cell walls through the metabolism of microorganisms, release active ingredients such as polysaccharides and flavonoids, and significantly improve bioavailability. However, the existing technology mostly uses the method of drying and crushing after fermentation to make feed additives. Although this method can retain some active ingredients, it is easy to cause ingredient degradation due to environmental factors during storage and use, and it is difficult to accurately control the content of effective ingredients. The post-fermentation extraction process can break through the above limitations. Through targeted extraction technology, it can efficiently enrich the active substances produced during the fermentation process, such as polysaccharides, flavonoids, organic acids, etc., and remove impurities and ineffective ingredients, so that the product has a higher content of active ingredients, stronger stability, and is more conducive to accurate addition and quality control.

[0004] The intestinal tract is an important organ for animal nutrition absorption and immune defense, and its flora balance directly affects growth performance and health status. In current breeding, factors such as stress and feed conversion often lead to intestinal flora disorder, causing diarrhea, growth retardation and other problems. Conventional regulatory measures such as adding a single probiotic have limited effects, while the combination of Astragalus (replenishing qi and strengthening the exterior), Codonopsis (strengthening the spleen and promoting fluid), Poria (diuresis and infiltration), Tangerine peel (regulating qi and digestion), and Pueraria root (relieve fever and promote fluid) with compound probiotics, using solid-state fermentation technology to improve the cell wall breaking rate of plants. Then, through step-by-step extraction, the extraction rate of the main active ingredients is improved. The extract obtained by solid-state fermentation and extraction technology has high active ingredients, and works together with probiotic metabolites (organic acids, antimicrobial peptides, etc.), which can regulate the intestinal flora structure at multiple targets, enhance the mucosal immune barrier, and fundamentally improve the health level of animals. Therefore, the development of such fermented Chinese medicine feed additives has important application value. Summary of the invention

[0005] The present invention aims to solve the technical problems in the prior art, and provides a fermented traditional Chinese medicine feed additive, its preparation method and application. This additive can effectively regulate the balance of animal intestinal flora, enhance immunity, promote the growth and development of animals, and has no antibiotic residue, being safe and environmentally friendly.

[0006] To achieve the above object, the specific technical solutions of the present invention are as follows:

[0007] In the first aspect of the present invention, a fermented traditional Chinese medicine feed additive is provided. The additive is prepared by solid-state fermentation from a traditional Chinese medicine composition, wheat bran, soybean meal, a compound bacterial agent and purified water.

[0008] The raw material components in the additive are as follows by weight: 60 - 90 parts of the traditional Chinese medicine composition, 5 - 20 parts of wheat bran, 2.5 - 10 parts of soybean meal, 0.5 - 5 parts of the compound bacterial agent, and 20 - 40 parts of purified water.

[0009] Preferably, the ratio of the traditional Chinese medicine composition to wheat bran and soybean meal is 85:10:5.

[0010] The traditional Chinese medicine composition includes the following raw materials by weight: 20 - 40 parts of Astragalus membranaceus, 10 - 30 parts of Poria cocos, 10 - 30 parts of Codonopsis pilosula, 5 - 20 parts of Citrus reticulata Blanco, and 5 - 20 parts of Pueraria lobata.

[0011] Preferably, the traditional Chinese medicine composition includes the following raw materials by weight: 25 parts of Astragalus membranaceus, 18 parts of Poria cocos, 18 parts of Codonopsis pilosula, 12 parts of Citrus reticulata Blanco, and 12 parts of Pueraria lobata.

[0012] The weight ratio of Bacillus subtilis, Lactobacillus plantarum and Aspergillus niger in the compound bacterial agent is 1:(1 - 2):(0.5 - 1).

[0013] Preferably, the weight ratio of Bacillus subtilis, Lactobacillus plantarum and Aspergillus niger in the compound bacterial agent is 1:1.5:0.8.

[0014] The viable bacteria counts in the compound bacterial agent are respectively: Bacillus subtilis ≥ 1.0×10 9 CFU / g, Lactobacillus plantarum ≥ 1.0×10 9 CFU / g, Aspergillus niger ≥ 1.0×10 9 CFU / g.

[0015] The extract of the additive after fermentation, stepwise extraction and spray drying is in powder form, and its addition amount in animal diets is 0.5 - 2.0%.

[0016] In the second aspect of the present invention, a preparation method of the above-mentioned fermented traditional Chinese medicine feed additive is provided, including the following steps:

[0017] S1: Wash the prescribed amount of traditional Chinese medicine composition, dry it, crush it through a 40-mesh sieve, and mix it evenly with wheat bran and soybean meal as the fermentation substrate;

[0018] S2: Add deionized water, adjust the water content of the fermentation substrate to 30%-50%, and sterilize it;

[0019] S3: Mix each single strain according to a preset ratio, adjust the viable count of each strain ≥ 10 9 CFU / g, and obtain a compound microbial agent after activation;

[0020] S4: After the fermentation substrate is cooled, inoculate 1-10% (v / m) of the compound microbial agent and stir evenly;

[0021] S5: Ferment at 30-37 °C for 4-7 days;

[0022] S6: Add 70% ethanol with a volume 6-10 times that of the weight of the medicinal materials to the fermentation product, reflux and extract for 1-3 hours, filter, and reserve the filtrate; perform a second extraction on the medicinal residues, add water with a volume 6-10 times that of the total amount of the medicinal materials, boil and extract for 1-3 hours, filter, and reserve the filtrate; combine the two filtrates, concentrate under reduced pressure to recover ethanol, and continue to concentrate to a relative density of 1.10-1.15 at 70 ± 5 °C;

[0023] S7: Centrifuge the concentrated solution with a disk centrifuge and then spray-dry it to obtain a dry powder.

[0024] Preferably, the water content in S2 is 30%.

[0025] Preferably, the inoculation amount of the compound microbial agent in S4 is 4%.

[0026] Preferably, the fermentation temperature in S5 is 34 °C and the fermentation time is 5 days.

[0027] Preferably, the dosage of 70% ethanol in S6 is 8 times the volume, reflux and extract for 2 hours; add water with a volume 8 times that of the total amount of the medicinal materials for the second extraction, boil and extract for 2 hours; concentrate to a relative density of 1.13 at 70 ± 5 °C.

[0028] In the third aspect of the present invention, there is provided the application of the fermented traditional Chinese medicine feed additive in livestock and poultry breeding.

[0029] Preferably, the application of the feed additive in regulating the animal intestinal flora.

[0030] Preferably, the application of the feed additive in promoting animal growth.

[0031] Preferably, the application of the feed additive in preventing animal diarrhea.

[0032] Preferably, the additive is compounded with compound vitamins and trace elements for use in poultry or pigs.

[0033] For the first time, the present invention ferments and extracts five traditional Chinese medicinal materials, namely Astragalus membranaceus, Codonopsis pilosula, Poria cocos, Citrus reticulata Blanco and Pueraria lobata, in combination with Bacillus subtilis, Lactobacillus plantarum and Aspergillus niger in specific proportions to prepare a feed additive. Among them, Astragalus membranaceus can replenish qi and consolidate the exterior, and is the core of the compound prescription. Its components such as Astragalus polysaccharides, flavonoids and saponins can activate immune cells and enhance the disease resistance of the body; Codonopsis pilosula synergistically with Astragalus membranaceus to replenish qi in the spleen and lung, with an emphasis on "invigorating the spleen and promoting fluid production". Its polysaccharide components can promote the proliferation of intestinal probiotics, and at the same time contain active substances such as codonopside, which can inhibit inflammatory reactions and make up for the warm and dry nature of Astragalus membranaceus; Poria cocos can strengthen the spleen and promote diuresis to eliminate dampness, assisting Astragalus membranaceus and Codonopsis pilosula to supplement without stagnation. Its components such as Poria polysaccharides and triterpenoids have diuretic and anti-inflammatory effects, can regulate water metabolism, and improve the syndrome of spleen deficiency and dampness accumulation; Citrus reticulata Blanco can regulate qi and strengthen the spleen, dry dampness and resolve phlegm. Its volatile oil components can promote the secretion of digestive juices, and flavonoids can have antioxidant and anti-inflammatory effects, synergistically enhancing the overall medicinal effect; Pueraria lobata can relieve fever and promote fluid production, forming a dynamic balance of "tonifying with dredging" with the qi-elevating and yang-activating effect of Astragalus membranaceus. Its isoflavone components such as puerarin and daidzin can improve microcirculation.

[0034] In the compound bacterium agent, Bacillus subtilis can secrete various extracellular enzymes such as protease and amylase, which can effectively decompose the plant cell wall and promote the release of macromolecular active components such as Astragalus polysaccharides and codonopside; Lactobacillus plantarum produces β-glucosidase during fermentation, which can hydrolyze glycosidic bonds, accelerate the precipitation of isoflavone components such as puerarin and daidzin, and reduce the pH value of the fermentation system by metabolizing organic acids, enhancing the stability of Poria polysaccharides and flavonoids; Aspergillus niger, as a high-efficiency enzyme-producing strain, can secrete cellulase, pectinase, etc., which can damage the cell structure of traditional Chinese medicinal materials and significantly improve the dissolution efficiency of components such as volatile oil of Citrus reticulata Blanco and Astragalus saponins. The three work together to significantly increase the content and bioavailability of active components in the fermentation product.

[0035] Beneficial effects:

[0036] 1. After the five traditional Chinese medicines, namely Astragalus membranaceus, Codonopsis pilosula, Poria cocos, Citrus reticulata Blanco and Pueraria lobata, are fermented by a compound bacterium (a combination of Bacillus subtilis, Lactobacillus plantarum and Aspergillus niger in specific proportions), the proteins are degraded into small peptides and amino acids, and the polysaccharides are degraded into monosaccharides or oligosaccharides, which are more easily absorbed. Acting together with the metabolites of probiotics (organic acids, antibacterial peptides, etc.), it can improve the efficiency of immune activation and anti-inflammatory regulation, and at the same time enhance the intestinal barrier function and antioxidant activity.

[0037] 2. Multiple efficacy verification: Verified by animal tests, the feed additive of the present invention has multiple efficacies. In terms of growth performance, it can significantly increase the daily weight gain of broilers and reduce the feed-to-weight ratio; in terms of intestinal health, it can significantly increase the number of intestinal lactic acid bacteria, reduce the number of Escherichia coli, and reduce the incidence of diarrhea; in terms of immune regulation, it can significantly increase the serum IgG content, fully demonstrating the comprehensive effect advantages of the products of the present invention.

[0038] 3. The fermented traditional Chinese medicine feed additive provided by the present invention realizes multiple efficacies of regulating intestinal flora, enhancing immunity, and promoting growth through innovative formula design and optimized preparation process, has a significant antibiotic substitution effect, and provides a new solution for green and healthy breeding. Specific embodiments

[0039] The following will further elaborate on the above content through specific embodiments in the form of examples. However, this should not be construed as limiting the scope of the above subject matter to the following examples. All technologies implemented based on the content of the present invention fall within the scope.

[0040] Example 1

[0041] A fermented traditional Chinese medicine feed additive, the preparation method of which includes the following steps: Weigh astragalus membranaceus, poria cocos, codonopsis pilosula, citrus reticulata peel, and pueraria lobata according to the ratio of 25:18:18:12:12, respectively, crush them and pass through a 40-mesh sieve and mix to obtain a traditional Chinese medicine composition; then evenly mix the traditional Chinese medicine composition with wheat bran and soybean meal as the fermentation substrate, and the ratio of the traditional Chinese medicine composition to wheat bran and soybean meal is 85:10:5. Add deionized water to the fermentation substrate to make its water content 30%, and sterilize; mix each single strain according to a preset ratio, adjust the viable count of each strain ≥ 10 9 CFU / g, and obtain a compound bacterium agent after activation (the ratio of bacillus subtilis, lactobacillus plantarum, and aspergillus niger is 1:1.5:0.8); after the fermentation substrate is cooled, inoculate 4% of the compound bacterium agent, stir evenly, put it into a fermentation bag, and ferment at 34 °C for 5 days. Add 70% ethanol with a volume 8 times that of the medicinal material weight to the fermentation product, reflux and extract for 2 hours, filter, and reserve the filtrate; perform a second extraction on the medicinal residues, add water with a volume 8 times that of the total amount of the medicinal materials, boil and extract for 2 hours, filter, and reserve the filtrate. Combine the two filtrates, concentrate under reduced pressure to recover ethanol, and continue to concentrate to a relative density of 1.13 at 70 ± 5 °C. Centrifuge the concentrated solution with a disc centrifuge and then spray dry to obtain a dry powder.

[0042] Example 2

[0043] A fermented traditional Chinese medicine feed additive, and its preparation method comprises the following steps: Weigh astragalus membranaceus, poria cocos, codonopsis pilosula, citrus reticulata peel, and pueraria lobata in a ratio of 20:10:10:5:5, crush them respectively, sieve through a 40-mesh sieve and mix to obtain a traditional Chinese medicine composition; then mix the traditional Chinese medicine composition with wheat bran and soybean meal evenly as a fermentation substrate, and the ratio of traditional Chinese medicine to wheat bran and soybean meal is 85:10:5. Add deionized water to the fermentation substrate to make its water content 30%, and sterilize it; mix each single strain according to a preset ratio, adjust the viable count of each strain to be ≥ 10 9 CFU / g, and obtain a compound microbial agent after activation (the ratio of bacillus subtilis, lactobacillus plantarum, and aspergillus niger is 1:1.5:0.8); after the fermentation substrate is cooled, inoculate 4% of the compound microbial agent, stir evenly, put it into a fermentation bag, and ferment at 34°C for 5 days. Add 70% ethanol with a volume 8 times that of the weight of the medicinal materials to the fermentation product, reflux and extract for 2 hours, filter, and reserve the filtrate; perform a second extraction on the medicinal residues, add water with a volume 8 times that of the total amount of the medicinal materials, boil and extract for 2 hours, filter, and reserve the filtrate. Combine the two filtrates, concentrate under reduced pressure to recover ethanol, and continue to concentrate to a relative density of 1.13 at 70 ± 5°C. Centrifuge the concentrated solution with a disc centrifuge and then spray-dry to obtain a dry powder.

[0044] Example 3

[0045] A fermented traditional Chinese medicine feed additive, and its preparation method comprises the following steps: Weigh astragalus membranaceus, poria cocos, codonopsis pilosula, citrus reticulata peel, and pueraria lobata in a ratio of 40:30:30:20:20, crush them respectively, sieve through a 40-mesh sieve and mix to obtain a traditional Chinese medicine composition; then mix the traditional Chinese medicine composition with wheat bran and soybean meal evenly as a fermentation substrate, and the ratio of traditional Chinese medicine to wheat bran and soybean meal is 85:10:5. Add deionized water to the fermentation substrate to make its water content 30%, and sterilize it; mix each single strain according to a preset ratio, adjust the viable count of each strain to be ≥ 10 9 CFU / g, and obtain a compound microbial agent after activation (the ratio of bacillus subtilis, lactobacillus plantarum, and aspergillus niger is 1:1.5:0.8); after the fermentation substrate is cooled, inoculate 4% of the compound microbial agent, stir evenly, put it into a fermentation bag, and ferment at 34°C for 5 days. Add 70% ethanol with a volume 8 times that of the weight of the medicinal materials to the fermentation product, reflux and extract for 2 hours, filter, and reserve the filtrate; perform a second extraction on the medicinal residues, add water with a volume 8 times that of the total amount of the medicinal materials, boil and extract for 2 hours, filter, and reserve the filtrate. Combine the two filtrates, concentrate under reduced pressure to recover ethanol, and continue to concentrate to a relative density of 1.13 at 70 ± 5°C. Centrifuge the concentrated solution with a disc centrifuge and then spray-dry to obtain a dry powder.

[0046] Comparative Example 1

[0047] A fermented traditional Chinese medicine feed additive, and its preparation method includes the following steps: Weigh astragalus membranaceus, poria cocos, codonopsis pilosula, atractylodes macrocephala, and Dioscorea opposita in a ratio of 25:18:18:12:12, respectively. After pulverizing, sieve through a 40-mesh sieve and mix to obtain a traditional Chinese medicine composition. Then, uniformly mix the traditional Chinese medicine composition with wheat bran and soybean meal as a fermentation substrate, and the ratio of the traditional Chinese medicine composition to wheat bran and soybean meal is 85:10:5. Add deionized water to the fermentation substrate to make its water content 30%, and sterilize. Mix each single strain according to a preset ratio, adjust the viable count of each strain to ≥ 10 9 CFU / g, and obtain a compound bacterium agent after activation (the ratio of Bacillus subtilis, Lactobacillus plantarum, and Aspergillus niger is 1:1.5:0.8); After the fermentation substrate is cooled, inoculate 4% of the compound bacterium agent, stir evenly, put it into a fermentation bag, and ferment at 34 °C for 5 days. Add 70% ethanol with a volume 8 times that of the weight of the medicinal materials to the fermentation product, reflux and extract for 2 hours, filter, and reserve the filtrate; Perform a second extraction on the medicinal residues, add water with a volume 8 times that of the total amount of the medicinal materials, boil and extract for 2 hours, filter, and reserve the filtrate. Combine the two filtrates, concentrate under reduced pressure to recover ethanol, and continue to concentrate to a relative density of 1.13 at 70 ± 5 °C. Centrifuge the concentrated solution with a disc centrifuge and then spray dry to obtain a dry powder.

[0048] Comparative Example 2

[0049] A fermented traditional Chinese medicine feed additive, and its preparation method includes the following steps: Weigh astragalus membranaceus, poria cocos, codonopsis pilosula, citrus reticulata blanco, and pueraria lobata in a ratio of 25:18:18:12:12, respectively. After pulverizing, sieve through a 40-mesh sieve and mix to obtain a traditional Chinese medicine composition. Then, uniformly mix the traditional Chinese medicine composition with wheat bran and soybean meal as a fermentation substrate, and the ratio of the traditional Chinese medicine composition to wheat bran and soybean meal is 85:10:5. Add deionized water to the fermentation substrate to make its water content 30%, and sterilize. Mix each single strain according to a preset ratio, adjust the viable count of each strain to ≥ 10 9 CFU / g, and obtain a compound bacterium agent after activation (the ratio of Bacillus coagulans, Lactobacillus lactis, and Saccharomyces cerevisiae is 1:1.5:0.8); After the fermentation substrate is cooled, inoculate 4% of the compound bacterium agent, stir evenly, put it into a fermentation bag, and ferment at 34 °C for 5 days. Add 70% ethanol with a volume 8 times that of the weight of the medicinal materials to the fermentation product, reflux and extract for 2 hours, filter, and reserve the filtrate; Perform a second extraction on the medicinal residues, add water with a volume 8 times that of the total amount of the medicinal materials, boil and extract for 2 hours, filter, and reserve the filtrate. Combine the two filtrates, concentrate under reduced pressure to recover ethanol, and continue to concentrate to a relative density of 1.13 at 70 ± 5 °C. Centrifuge the concentrated solution with a disc centrifuge and then spray dry to obtain a dry powder.

[0050] Comparative Example 3

[0051] A fermented traditional Chinese medicine feed additive, and its preparation method includes the following steps: Weigh astragalus membranaceus, poria cocos, codonopsis pilosula, citrus reticulata blanco, and pueraria lobata in a ratio of 25:18:18:12:12, crush them separately, and sieve through a 40-mesh sieve and mix to obtain a traditional Chinese medicine composition; then mix the traditional Chinese medicine composition evenly with wheat bran and soybean meal as a fermentation substrate, and the ratio of the traditional Chinese medicine composition to wheat bran and soybean meal is 85:10:5. Add deionized water to the fermentation substrate to make its water content 30%, and sterilize; mix each single strain according to a preset ratio, adjust the viable count of each strain ≥ 10 9 CFU / g, and obtain a compound microbial agent after activation (the ratio of bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus plantarum, and lactobacillus acidophilus is 1:1:0.8:1:1:1); after the fermentation substrate is cooled, inoculate 4% of the compound microbial agent, stir evenly, put it into a fermentation bag, and ferment at 34°C for 5 days. Add 70% ethanol with a volume 8 times that of the medicinal materials to the fermentation product, reflux and extract for 2 hours, filter, and reserve the filtrate; perform a second extraction on the medicinal residues, add water with a volume 8 times that of the total amount of the medicinal materials, boil and extract for 2 hours, filter, and reserve the filtrate. Combine the two filtrates, concentrate under reduced pressure to recover ethanol, and continue to concentrate to a relative density of 1.13 at 70 ± 5°C. Centrifuge the concentrated solution with a disc centrifuge and then spray-dry to obtain a dry powder.

[0052] Control Example 4

[0053] A fermented traditional Chinese medicine feed additive, and its preparation method includes the following steps: Weigh astragalus membranaceus, poria cocos, codonopsis pilosula, citrus reticulata blanco, pueraria lobata, angelica sinensis, and ligusticum wallichii in a ratio of 25:12:12:10:10:8:8, crush them separately, and sieve through a 40-mesh sieve and mix to obtain a traditional Chinese medicine composition; then mix the traditional Chinese medicine composition evenly with wheat bran and soybean meal as a fermentation substrate, and the ratio of the traditional Chinese medicine composition to wheat bran and soybean meal is 85:10:5. Add sterile water to the fermentation substrate to make its water content 30%, and sterilize; mix each single strain according to a preset ratio, adjust the viable count of each strain ≥ 10 9 CFU / g, and obtain a compound microbial agent after activation (the ratio of bacillus subtilis, lactobacillus plantarum, and aspergillus niger is 1:1.5:0.8); after the fermentation substrate is cooled, inoculate 4% of the compound microbial agent, stir evenly, put it into a fermentation bag, and ferment at 34°C for 5 days. Add 70% ethanol with a volume 8 times that of the medicinal materials to the fermentation product, reflux and extract for 2 hours, filter, and reserve the filtrate; perform a second extraction on the medicinal residues, add water with a volume 8 times that of the total amount of the medicinal materials, boil and extract for 2 hours, filter, and reserve the filtrate. Combine the two filtrates, concentrate under reduced pressure to recover ethanol, and continue to concentrate to a relative density of 1.13 at 70 ± 5°C. Centrifuge the concentrated solution with a disc centrifuge and then spray-dry to obtain a dry powder.

[0054] Control Example 5

[0055] A fermented traditional Chinese medicine feed additive, and its preparation method includes the following steps: Weigh astragalus membranaceus, poria cocos, codonopsis pilosula, citrus reticulata blanco, and pueraria lobata in a ratio of 25:18:18:12:12, crush them respectively, and sieve through a 40-mesh sieve and mix to obtain a traditional Chinese medicine composition; then mix the traditional Chinese medicine composition with wheat bran and soybean meal evenly as a fermentation substrate, and the ratio of the traditional Chinese medicine composition to wheat bran and soybean meal is 85:10:5. Add sterile water to the fermentation substrate to make its water content 30%, and sterilize; mix each single strain according to a preset ratio, adjust the viable count of each strain ≥ 10 9 CFU / g, and obtain a compound microbial agent after activation (the ratio of bacillus subtilis, lactobacillus plantarum, and aspergillus niger is 1:1.5:0.8); after the fermentation substrate cools, inoculate 4% of the compound microbial agent, stir evenly, put it into a fermentation bag, ferment at 34°C for 5 days, and dry it with a blast dryer until the water content is 10% to obtain a fermentation product.

[0056] Control Example 6

[0057] A fermented traditional Chinese medicine feed additive, and its preparation method includes the following steps: Weigh astragalus membranaceus, poria cocos, codonopsis pilosula, citrus reticulata blanco, and pueraria lobata in a ratio of 25:18:18:12:12, crush them respectively, and sieve through a 40-mesh sieve and mix to obtain a traditional Chinese medicine composition; add 70% ethanol with a volume 8 times that of the medicinal materials, reflux and extract for 2 hours, filter, and reserve the filtrate; perform a second extraction on the medicinal residues, add water with a volume 8 times that of the total amount of the medicinal materials, boil and extract for 2 hours, filter, and reserve the filtrate. Combine the two filtrates, concentrate under reduced pressure to recover ethanol, and continue to concentrate to a relative density of 1.13 at 70 ± 5°C. Centrifuge the concentrated solution with a disc centrifuge and then spray dry to obtain a dry powder.

[0058] Test Example 1 Verification of the extraction effect of active ingredients fermented by different proportions of strains

[0059] In the present invention, a compound microbial agent is composed of three feed microorganisms, namely bacillus subtilis, lactobacillus plantarum, and aspergillus niger, in different proportions, with a total of 6 groups, as shown in Table 1. The formulation and dosage of the traditional Chinese medicine composition, wheat bran, and soybean meal, as well as the inoculation amount of the compound microbial agent and the fermentation and extraction methods, are the same as those in Example 1. Detect the total sugar and total flavonoid contents in the extract. The total polysaccharide content is determined by the phenol-sulfuric acid method, and the flavonoid content is determined by the sodium nitrite-aluminum nitrate colorimetric method. The results are shown in Table 2.

[0060] Table 1 Proportion grouping of strains in the compound microbial agent

[0061] 。

[0062] Table 2 Total amounts of polysaccharides and flavonoids obtained by fermenting strains in different proportions

[0063]

[0064] As can be seen from Table 2, the total polysaccharide content was the highest in Group 5 and was significantly higher than that in other groups; the total flavonoid content was the highest in Group 3 and the second highest in Group 5. In summary, solid fermentation with the compound bacterial agent in Group 5 had the best effect on improving the active ingredients of the traditional Chinese medicine compound.

[0065] Experimental Example 2 Extraction effect of the active ingredients of the product of the embodiment of the present invention

[0066] The total polysaccharide content was determined by the phenol-sulfuric acid method, and the flavonoid content was determined by the sodium nitrite-aluminum nitrate colorimetric method. The total sugar and total flavonoid contents in the extracts and fermentation products (Examples 1-3, Comparative Examples 1-6) were detected, and the results are shown in Table 3.

[0067] Table 3 Total polysaccharide and total flavonoid contents of products of different embodiments of the invention

[0068]

[0069] The experimental results showed that the comprehensive effect of the products in Examples 1-3 was the best, with relatively high contents of total polysaccharide and total flavonoid, and this was closely related to the traditional Chinese medicine formula and the composition of the compound bacterial agent.

[0070] Experimental Example 3 Effects of the product of the present invention on the weight gain performance and intestinal health of broilers

[0071] A total of 480 1-day-old white-feathered broilers were randomly selected in a chicken farm and randomly divided into 8 groups with 60 broilers in each group. The additives prepared in Examples 1-3 and Comparative Examples 1-4 were added to the basal diet at 1% of the feed weight, and the blank control group was fed with the basal diet. The test period was 42 days. The measurement results are shown in Tables 4 and 5.

[0072] Table 4 Effects of the product of the present invention on the growth performance and diarrhea of broilers

[0073]

[0074] Table 5 Effects of the product of the present invention on the intestinal health of broilers

[0075]

[0076] The above results show that the products of Examples 1-3 of the present invention have the best effects, especially Example 1 is more prominent. In terms of growth performance, the product of Example 1 performs the best, with the daily weight gain (65.1 g / d) increasing by 13.8% compared with the control group, and the feed-to-weight ratio decreasing by 10.4%. It is speculated that this is because the proportion of the traditional Chinese medicine compound is moderate, and the metabolites of probiotics and high-active components synergistically enhance digestion and absorption. In terms of intestinal health, the villus height in Example 1 increased significantly (+18.8%), and the proliferation of lactic acid bacteria increased (+18.1%), indicating that the extract optimized the intestinal microecology. In terms of enhancing immunity, the serum IgG in Example 1 increased by 66.7%, which is related to the immune activation effect of polysaccharides and flavonoid components. The product of the present invention has broad prospects as an industrialization formula.

Claims

1. A fermented traditional Chinese medicine feed additive, characterized in that, The additive is prepared by solid-state fermentation from the following raw materials in parts by mass: 60-90 parts of traditional Chinese medicine composition, 5-20 parts of wheat bran, 2.5-10 parts of soybean meal, 0.5-5 parts of compound bacterial agent, and 20-40 parts of purified water.

2. The additive according to claim 1, wherein The traditional Chinese medicine composition includes the following raw materials in parts by weight: 20-40 parts of Astragalus membranaceus, 10-30 parts of Poria cocos, 10-30 parts of Codonopsis pilosula, 5-20 parts of Citrus reticulata Blanco, and 5-20 parts of Pueraria lobata.

3. The additive according to claim 2, characterized in that, The traditional Chinese medicine composition includes the following raw materials in parts by weight: 25 parts of Astragalus membranaceus, 18 parts of Poria cocos, 18 parts of Codonopsis pilosula, 12 parts of Citrus reticulata Blanco, and 12 parts of Pueraria lobata.

4. The additive according to claim 1, characterized in that, In the compound bacterial agent, the weight ratio of Bacillus subtilis, Lactobacillus plantarum, and Aspergillus niger is 1:(1-2):(0.5-1).

5. The additive according to claim 4, characterized in that, In the compound bacterial agent, the weight ratio of Bacillus subtilis, Lactobacillus plantarum, and Aspergillus niger is 1:1.5:0.

8.

6. The additive according to claim 5, characterized in that, The viable counts of the live bacteria in the compound bacterial agent are respectively: Bacillus subtilis ≥ 1.0×10 9 CFU / g, Lactobacillus plantarum ≥ 1.0×10 9 CFU / g, Aspergillus niger ≥ 1.0×10 9 CFU / g.

7. The additive according to any one of claims 1-6, characterized in that, The extract after the additive is fermented, extracted step by step, and spray-dried is in powder form, and the addition amount in the animal diet is 0.5-2.0%.

8. The preparation method of a fermented traditional Chinese medicine feed additive according to any one of claims 1-6, comprising the following steps: S1: Wash, dry, and pulverize the prescription amount of traditional Chinese medicine composition through a 40-mesh sieve, and mix it evenly with wheat bran and soybean meal as the fermentation substrate; S2: Add deionized water to adjust the water content of the fermentation substrate to 30%-50%, and sterilize; S3: Mix each single strain according to a preset ratio, and adjust the viable count of each strain to be ≥ 10 9 CFU / g. After activation, a compound microbial agent is obtained; S4: After the fermentation substrate is cooled, inoculate 1-10% (v / m) of the compound bacterial agent and stir evenly; S5: Ferment at 30-37°C for 4-7 days; S6: Add 70% ethanol with a volume 6-10 times the weight of the medicinal materials to the fermentation product, reflux and extract for 1-3 hours, filter, and reserve the filtrate; perform a second extraction on the medicinal residues, add water with a volume 6-10 times the total amount of the medicinal materials, boil and extract for 1-3 hours, filter, and reserve the filtrate; combine the two filtrates, concentrate under reduced pressure to recover ethanol, and continue to concentrate to a relative density of 1.10-1.15 at 70±5°C; S7: Centrifuge the concentrated solution with a disc centrifuge and then spray-dry to obtain a dry powder.

9. The application of a fermented traditional Chinese medicine feed additive according to any one of claims 1-7 in the preparation of a feed for regulating animal intestinal flora, promoting animal growth, enhancing animal immunity, and preventing animal diarrhea.

10. The additive according to claim 9, characterized in that, The additive is compounded with compound vitamins and trace elements for use in poultry or pigs.

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