Compound essential oil with anti-aging effect and preparation method thereof

Abstract

The invention provides compound essential oil with an anti-aging effect and a preparation method thereof, and belongs to the technical field of essential oil. The preparation process comprises the following steps: preparing the compound essential oil; preparing a composite lipid nano inclusion compound; and preparing the compound essential oil. The pomegranate seeds and the leontopodium leontopodum are used for constructing a first defense line for light aging and environmental oxidation, the permanent flowers and the purple perilla are used for inhibiting inflammation and saccharification damage and preventing a corium layer structure from collapsing, and the ligusticum wallichii and the oriental cherry are used for improving skin color and texture from metabolism and microcirculation levels; through multi-target-point and multi-layer cooperation, the problems of color spots, looseness, dryness and inflammation-sensitive aging which are dominated by light aging can be obviously solved.

Description

Technical Field

[0001] The present invention relates to the technical field of essential oils, and particularly relates to a compound essential oil with anti-aging effects and a preparation method thereof. Background Art

[0002] With the continuous improvement of people's demands for skin health and beauty, the anti-aging skin care product market continues to grow. Among many skin care products, compound essential oils have attracted much attention due to their natural plant ingredients, good permeability, and unique skin care effects.

[0003] However, most of the current anti-aging essential oils on the market still have many limitations. On the one hand, in terms of the efficacy of ingredients, it is difficult for single or a few plant ingredients to comprehensively address the complex mechanisms of skin aging. Skin aging is often caused by the combined effects of multiple factors such as photoaging, oxidative stress, inflammatory response, glycation damage, and microcirculation disorders. Existing products cannot synergistically solve problems such as skin pigmentation, relaxation, dryness, and inflammatory sensitive aging from multiple targets and multiple levels, and it is difficult to achieve ideal anti-aging effects. On the other hand, at the technical level, traditional essential oil encapsulation technology cannot effectively protect thermosensitive ingredients, the inclusion complex has poor stability, and active ingredients are easily inactivated by oxygen and light during storage and use, and it is difficult to accurately penetrate the stratum corneum and target the delivery of active ingredients to the dermis to play a role, resulting in a significant reduction in efficacy.

[0004] Therefore, we propose a compound essential oil with anti-aging effects and a preparation method thereof to solve the above problems. Summary of the Invention

[0005] Aiming at the deficiencies of the existing technology, the purpose of the present invention is to provide a compound essential oil with anti-aging effects and a preparation method thereof.

[0006] A preparation method of a compound essential oil with anti-aging effects includes the following steps: S1: Preparation of compound plant essential oil Extract compound plant essential oil with pomegranate seeds, edelweiss, cherry blossoms, chuanxiong rhizome, perilla, and immortelle as raw materials; S2: Preparation of compound lipid nano-inclusion Add lecithin, tea saponin, and sophorolipid to deionized water to obtain a mixed solution, mix glyceryl distearate, caprylic / capric triglyceride, and compound plant essential oil to obtain an oil-phase solution, and mix and stir the oil-phase solution and the mixed solution, and ultrasonically solidify to obtain a compound lipid nano-inclusion; S3: Preparation of compound essential oil Mix phospholipids, glycerol, hydroxyethyl cellulose, polyglycerol monolaurate and trehalose to obtain Mixture I. After mixing an alcohol solvent, olive oil, jojoba oil, frankincense oil and a composite lipid nano-inclusion complex, add them to Mixture I to obtain Mixture II. Add deionized water to Mixture II and stir to mix to obtain a compound essential oil.

[0007] Further, the preparation of the composite plant essential oil in Step S1 specifically includes the following steps: S1.1: Weigh pomegranate seeds, edelweiss, cherry blossoms, chuanxiong, perilla and immortelle respectively and mix them. Perform freeze-drying treatment at a temperature of -20°C to -25°C for 15 - 20 h, and then perform low-temperature ultrafine pulverization treatment at -8°C to -10°C for 15 - 20 min to obtain a composite plant powder. S1.2: Place the composite plant powder in a round-bottom flask, add 10 - 12 times the amount of water, add cellulase to make the final concentration of cellulase 1 - 2%, then adjust the pH to 4.5 - 5, heat to 40 - 45°C, enzymatically hydrolyze for 30 - 40 min, then connect the distillation device and start distillation for 2 - 3 h, and collect the essential oil with an oil-water separator to obtain the composite plant essential oil.

[0008] Further, the preparation of the composite lipid nano-inclusion complex in Step S2 specifically includes the following steps: S2.1: Mix lecithin, tea saponin and sophorolipid and add them to deionized water, stir and mix for 20 - 30 min to obtain a mixed solution. S2.2: Mix 1 - 3 parts by weight of glyceryl distearate and 1 - 3 parts by weight of glyceryl caprylate at 73 - 75°C, then add 3 - 5 parts by weight of the composite plant essential oil and stir and mix at 200 - 300 rpm to obtain an oil-phase solution. S2.3: Under the condition of magnetic stirring at 1000 - 1200 r / min, drop the oil-phase solution into the mixed solution at 73 - 75°C, stir to form a primary emulsion, put the primary emulsion into an ultrasonic cell disruptor for ultrasonic dispersion, cool and solidify it in an ice-water bath for 30 - 40 min, and then filter it through a 0.45 μm microporous membrane to obtain the composite lipid nano-inclusion complex.

[0009] Further, the preparation of the compound essential oil in Step S3 specifically includes the following steps: S3.1: Mix 3 - 5 parts by weight of phospholipids, 1 - 3 parts by weight of glycerol, 1 - 3 parts by weight of hydroxyethyl cellulose, 3 - 9 parts by weight of polyglycerol monolaurate and 1 - 2 parts by weight of trehalose, heat to 65 - 70°C, and stir at 500 - 800 r / min for 8 - 10 min to obtain Mixture I. S3.2: Mix 2 - 4 parts by weight of alcohol solvent, 1 - 3 parts by weight of olive oil, 2 - 3 parts by weight of jojoba oil, 0.1 - 0.3 parts by weight of frankincense oil, and 25 - 30 parts by weight of composite lipid nanocapsules, then add them to Mixture Ⅰ. After stirring evenly, obtain Mixture Ⅱ; S3.3: Add 30 - 50 parts by weight of deionized water at 65 - 70 °C to Mixture Ⅱ, and then continue to stir at 20000 - 25000 r / min for 10 - 15 min to obtain the compound essential oil.

[0010] Furthermore, in step S1.1, the weight ratio of pomegranate seeds, leontopodium alpinum, cherry blossoms, ligusticum wallichii, perilla, and immortelle is (2 - 3):(1 - 2):(1 - 3):(2 - 3):(3 - 5):(2 - 3).

[0011] Furthermore, in the mixed solution of step S2.1, the mass fraction of lecithin is 2 - 3%, the mass fraction of tea saponin is 0.3 - 0.5%, and the mass fraction of sophorolipid is 0.2 - 0.3%.

[0012] Furthermore, in step S2.3, the volume ratio of the oil - phase solution to the mixed solution is 1:8 - 10.

[0013] Furthermore, in step S3.2, the alcohol solvent is propylene glycol.

[0014] A compound essential oil with anti - aging effect, which is prepared by the preparation method of a compound essential oil with anti - aging effect described in any one of the above.

[0015] Compared with the prior art, the present invention has at least the following beneficial effects: 1. In the present invention, pomegranate seeds enhance collagen synthesis by promoting the TGF - β pathway, and leontopodium alpinum reduces collagen degradation by inhibiting the generation of MMP - 1 and AGEs. The two work synergistically in a "synthesis - protection" two - way manner to form a closed - loop mechanism for delaying skin aging, significantly improving wrinkles and relaxation, and delaying skin aging caused by collagen loss. Immortelle is rich in fat - soluble antioxidant components, focusing on scavenging fat - soluble free radicals; perilla contains water - soluble components such as rosmarinic acid, which is more effective against water - soluble free radicals. The combination of the two covers oxidative damage inside and outside cells, can inhibit lipid peroxidation, reduce collagen decomposition caused by inflammation, and maintain the stability of the skin structure. Ligustrazine dilates capillaries, improves skin blood oxygen supply, and accelerates the clearance of metabolic wastes; cherry blossoms contain prunus serrulata flavonoids, which promote keratin renewal and reduce melanin deposition. The two work synergistically from the "microcirculation - keratin metabolism" level to improve dull skin color, age spots and other dull - type aging.

[0016] 2. The everlasting flower and perilla in the present invention cover lipid-soluble / water-soluble free radicals, the edelweiss protects mitochondria, blocks the source of oxidative stress, the pomegranate seeds promote synthesis, the edelweiss and everlasting flower inhibit degradation, the perilla assists in collagen production, maintains skin elasticity, the chuanxiong improves microcirculation, the cherry blossom accelerates cutin renewal, clears metabolic waste and pigment deposition, and each component works together to jointly solve the problems of age spots, relaxation, dryness and inflammatory sensitivity aging dominated by photoaging, achieving multi-dimensional anti-aging effects.

[0017] 3. The present invention encapsulates compound plant essential oils through a lipid bilayer. Lecithin and glycerides form a dense barrier, which can isolate oxygen and light, protect the thermosensitive components in the essential oils, and the nanoscale inclusion complex can penetrate the gaps of the stratum corneum and be targeted to the dermis layer to enhance the effects of chuanxiong in promoting microcirculation and edelweiss in repair. The saponin and sophorolipid added during the preparation of the inclusion complex can bind to lecithin and participate in the formation of the liposome membrane, making the nanostructure more compact and improving the stability of the inclusion complex. The synergistic effect of saponin and sophorolipid can improve the encapsulation ability of the inclusion complex for compound plant essential oils. On the one hand, the better emulsification and dispersion effects after adding saponin and sophorolipid enable more active ingredients to be encapsulated inside the nanostructure; on the other hand, saponin and sophorolipid act together on the surface of the nanoparticles, and optimizing the nanoparticle structure also helps to improve the encapsulation rate and reduce the leakage of active ingredients. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings incorporated herein and constituting a part of the specification illustrate embodiments of the present invention and, together with the specification, are further used to explain the principles of the present invention and enable those skilled in the relevant art to implement and use the present invention.

[0019] Figure 1 It is a flowchart of a preparation method of a compound essential oil with anti-aging effects adopted in an embodiment of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0020] The following describes in detail a preparation method of a compound essential oil with anti-aging effects provided by the present invention in combination with the drawings and specific embodiments. At the same time, it should be noted here that in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments, and those skilled in the art can also adopt other alternative methods for some well-known technologies; moreover, the drawing part is only for more specifically describing the embodiments and is not intended to specifically limit the present invention.

[0021] Example 1 A preparation method of a compound essential oil with anti-aging effects, as Figure 1 shown, includes the following steps: S1: Preparation of compound plant essential oils S1.1: Weigh 2 parts by weight of pomegranate seeds, 1 part by weight of Leontopodium alpinum, 1 part by weight of cherry blossoms, 2 parts by weight of Ligusticum wallichii, 3 parts by weight of Perilla frutescens, and 2 parts by weight of Helichrysum stoechas respectively, mix them, conduct freeze-drying treatment at a temperature of -20°C for 15 h, and then conduct low-temperature ultrafine grinding treatment at -8°C for 15 min to obtain a composite plant powder; S1.2: Place the composite plant powder in a round-bottom flask, add 10 times the amount of water, add cellulase to make the final concentration of cellulase 1%, then adjust the pH to 4.5, heat to 40°C, after enzymatic hydrolysis for 30 min, connect the distillation device and start distillation for 2 h, and collect the essential oil with an oil-water separator to obtain a composite plant essential oil; S2: Preparation of composite lipid nanocapsules S2.1: Mix lecithin, tea saponin, and sophorolipid and add them to deionized water, stir and mix for 20 min to obtain a mixed solution. In the mixed solution, the mass fraction of lecithin is 2%, the mass fraction of tea saponin is 0.3%, and the mass fraction of sophorolipid is 0.2%; S2.2: Mix 1 part by weight of glyceryl distearate and 1 part by weight of caprylic / capric triglyceride at 73°C, and then add 3 parts by weight of the composite plant essential oil, and stir and mix at 200 rpm to obtain an oil-phase solution; S2.3: Under the condition of magnetic stirring at 1000 r / min, drop the oil-phase solution into the mixed solution at 73°C. The volume ratio of the oil-phase solution to the mixed solution is 1:8, stir to form a primary emulsion, put the primary emulsion into an ultrasonic cell disruptor for ultrasonic dispersion, cool and solidify it in an ice-water bath for 30 min, and then filter it through a 0.45-μm microporous membrane to obtain a composite lipid nanocapsule; S3: Preparation of compound essential oil S3.1: Mix 3 parts by weight of phospholipids, 1 part by weight of glycerol, 1 part by weight of hydroxyethyl cellulose, 3 parts by weight of polyglycerol monolaurate, and 1 part by weight of trehalose, heat to 65°C, and stir at 500 r / min for 8 min to obtain mixture I; S3.2: Mix 2 parts by weight of propylene glycol, 1 part by weight of olive oil, 2 parts by weight of jojoba oil, 0.1 part by weight of frankincense oil, and 25 parts by weight of the composite lipid nanocapsule, and then add them to mixture I, stir evenly to obtain mixture II; S3.3: Add 30 parts by weight of deionized water at 65°C to mixture II, and then continue to stir at 20000 r / min for 10 min to obtain a compound essential oil.

[0022] Example 2 A preparation method of a compound essential oil with anti-aging efficacy, as Figure 1 shown, includes the following steps: S1: Preparation of composite plant essential oil S1.1: Weigh 3 parts by weight of pomegranate seeds, 2 parts by weight of Leontopodium leontopodioides, 3 parts by weight of cherry blossoms, 3 parts by weight of Ligusticum chuanxiong, 5 parts by weight of perilla, and 3 parts by weight of Helichrysum italicum respectively, mix them, conduct freeze-drying treatment at -20 °C for 15 h, and then conduct low-temperature ultrafine grinding treatment at -8 °C for 15 min to obtain a composite plant powder; S1.2: Place the composite plant powder in a round-bottom flask, add 12 times the amount of water, add cellulase to make the final concentration of cellulase 2%, then adjust the pH to 5, heat to 40 °C, after enzymatic hydrolysis for 30 min, connect the distillation device and start distillation for 2 h, and collect the essential oil with an oil-water separator to obtain a composite plant essential oil; S2: Preparation of composite lipid nanoparticles S2.1: Mix lecithin, tea saponin, and sophorolipid and add them to deionized water, stir and mix for 20 min to obtain a mixed solution. In the mixed solution, the mass fraction of lecithin is 3%, the mass fraction of tea saponin is 0.5%, and the mass fraction of sophorolipid is 0.3%; S2.2: Mix 3 parts by weight of glyceryl distearate and 3 parts by weight of caprylic / capric triglyceride at 73 °C, and then add 5 parts by weight of the composite plant essential oil, and stir and mix at 200 rpm to obtain an oil-phase solution; S2.3: Under the condition of magnetic stirring at 1000 r / min, drop the oil-phase solution into the mixed solution at 73 °C. The volume ratio of the oil-phase solution to the mixed solution is 1:10, stir to form a primary emulsion, put the primary emulsion into an ultrasonic cell disruptor for ultrasonic dispersion, cool and solidify in an ice-water bath for 30 min, and then filter through a 0.45 μm microporous filter membrane to obtain a composite lipid nanoparticle; S3: Preparation of compound essential oil S3.1: Mix 5 parts by weight of phospholipid, 3 parts by weight of glycerol, 3 parts by weight of hydroxyethyl cellulose, 9 parts by weight of polyglyceryl monolaurate, and 2 parts by weight of trehalose, heat to 65 °C, and stir at 500 r / min for 8 min to obtain mixture Ⅰ; S3.2: Mix 4 parts by weight of propylene glycol, 3 parts by weight of olive oil, 3 parts by weight of jojoba oil, 0.3 parts by weight of frankincense oil, and 30 parts by weight of the composite lipid nanoparticle, and then add them to mixture Ⅰ, stir evenly to obtain mixture Ⅱ; S3.3: Add 50 parts by weight of deionized water at 65 °C to mixture Ⅱ, and then continue to stir at 20000 r / min for 10 min to obtain a compound essential oil.

[0023] Example 3 A preparation method of a compound essential oil with anti-aging efficacy, as Figure 1 shown, includes the following steps: S1: Preparation of composite plant essential oil S1.1: Weigh 2 parts by weight of pomegranate seeds, 1 part by weight of Leontopodium leontopodioides, 1 part by weight of cherry blossoms, 2 parts by weight of Ligusticum chuanxiong, 3 parts by weight of perilla, and 2 parts by weight of Helichrysum italicum respectively, mix them, perform freeze-drying treatment at a temperature of -25°C for 20 h, and then perform low-temperature ultrafine grinding treatment at -10°C for 20 min to obtain a composite plant powder; S1.2: Place the composite plant powder in a round-bottom flask, add 10 times the amount of water, add cellulase to make the final concentration of cellulase 1%, then adjust the pH to 4.5, heat to 45°C, after enzymatic hydrolysis for 40 min, connect the distillation device and start distillation for 3 h, and collect the essential oil with an oil-water separator to obtain a composite plant essential oil; S2: Preparation of composite lipid nanocapsules S2.1: Mix lecithin, tea saponin, and sophorolipid and add them to deionized water, stir and mix for 30 min to obtain a mixed solution. The mass fraction of lecithin in the mixed solution is 2%, the mass fraction of tea saponin is 0.3%, and the mass fraction of sophorolipid is 0.2%; S2.2: Mix 1 part by weight of glyceryl distearate and 1 part by weight of caprylic / capric triglyceride at 75°C, then add 3 parts by weight of the composite plant essential oil, and stir and mix at 300 rpm to obtain an oil-phase solution; S2.3: Under the condition of magnetic stirring at 1200 r / min, drop the oil-phase solution into the mixed solution at 75°C. The volume ratio of the oil-phase solution to the mixed solution is 1:8, stir to form a primary emulsion, put the primary emulsion into an ultrasonic cell disruptor for ultrasonic dispersion, cool and solidify it under the condition of an ice-water bath for 40 min, and then filter it through a 0.45-μm microporous filter membrane to obtain a composite lipid nanocapsule; S3: Preparation of compound essential oil S3.1: Mix 3 parts by weight of phospholipids, 1 part by weight of glycerol, 1 part by weight of hydroxyethyl cellulose, 3 parts by weight of polyglyceryl monolaurate, and 1 part by weight of trehalose, heat to 70°C, and stir at 800 r / min for 10 min to obtain mixture I; S3.2: Mix 2 parts by weight of propylene glycol, 1 part by weight of olive oil, 2 parts by weight of jojoba oil, 0.1 part by weight of frankincense oil, and 25 parts by weight of the composite lipid nanocapsule, and then add them to mixture I, stir evenly to obtain mixture II; S3.3: Add 30 parts by weight of deionized water at 70°C to mixture II, and then continue to stir at 25000 r / min for 15 min to obtain a compound essential oil.

[0024] Comparative Example 1 Compared with Example 1, the difference in Comparative Example 1 is that in Comparative Example 1, the pomegranate seeds in step S1.1 are replaced with Leontopodium leontopodioides, and the compound essential oil is prepared with the remaining steps unchanged, denoted as Comparative Example 1.

[0025] Comparative Example 1 Compared with Example 1, the difference in Comparative Example 2 is that in Comparative Example 2, the edelweiss in step S1.1 is replaced with pomegranate seeds, and the remaining steps remain unchanged to prepare the compound essential oil, denoted as Comparative Example 2.

[0026] Comparative Example 3 Compared with Example 1, the difference in Comparative Example 3 is that in Comparative Example 3, the everlasting flower in step S1.1 is replaced with perilla, and the remaining steps remain unchanged to prepare the compound essential oil, denoted as Comparative Example 3.

[0027] Comparative Example 4 Compared with Example 1, the difference in Comparative Example 4 is that in Comparative Example 4, the perilla in step S1.1 is replaced with everlasting flower, and the remaining steps remain unchanged to prepare the compound essential oil, denoted as Comparative Example 4.

[0028] Comparative Example 5 Compared with Example 1, the difference in Comparative Example 5 is that in Comparative Example 5, the chuanxiong rhizome in step S1.1 is replaced with cherry blossom, and the remaining steps remain unchanged to prepare the compound essential oil, denoted as Comparative Example 5.

[0029] Comparative Example 6 Compared with Example 1, the difference in Comparative Example 6 is that in Comparative Example 6, the cherry blossom in step S1.1 is replaced with chuanxiong rhizome, and the remaining steps remain unchanged to prepare the compound essential oil, denoted as Comparative Example 6.

[0030] Comparative Example 7 Compared with Example 1, the difference in Comparative Example 7 is that in Comparative Example 7, the tea saponin in step S2.1 is replaced with sophorolipid, and the remaining steps remain unchanged to prepare the compound essential oil, denoted as Comparative Example 7.

[0031] Comparative Example 8 Compared with Example 1, the difference in Comparative Example 8 is that in Comparative Example 8, the sophorolipid in step S2.1 is replaced with tea saponin, and the remaining steps remain unchanged to prepare the compound essential oil, denoted as Comparative Example 8.

[0032] Eighty people aged 30 - 40 with facial skin problems such as age spots, wrinkles, and facial sagging were selected as volunteers. They were randomly divided into 8 groups and applied the compound essential oils prepared in Examples 1 - 3 and Comparative Examples 1 - 6 once each morning and evening after cleaning their faces every day. The skin of the volunteers was tested before and after use. After 30 days, the trial effects were statistically analyzed. An improvement in age spots, wrinkles, and facial sagging of > 30% was considered a significant effect; an improvement of 5 - 30% was considered effective; and an improvement of < 5% was considered ineffective.

[0033] Table 1. Trial Effects of Examples 1 - 3 and Comparative Examples 1 - 6 Number of people with significant effects Number of people with effects Number of people with no effects Example 1 8 2 0 Example 2 7 3 0 Example 3 8 2 0 Comparative Example 1 2 6 2 Comparative Example 2 3 4 3 Comparative Example 3 2 5 3 Comparative Example 4 2 4 4 Comparative Example 5 1 5 4 Comparative Example 6 1 6 3 As can be seen from the data in Table 1, the compound essential oil prepared by the present invention can significantly improve the symptoms of skin pigmentation, wrinkles, and facial relaxation. The improvement effects of Comparative Examples 1-2 on skin pigmentation, wrinkles, and facial relaxation are poorer than those of Examples 1-3, indicating that pomegranate seeds and leontopodium alpinum can achieve a synergistic effect in delaying and improving skin aging. As can be seen from the data in Comparative Examples 3-4, the combined use of perilla and helichrysum can improve the symptoms of skin pigmentation, wrinkles, and facial relaxation. As can be seen from the data in Comparative Examples 5-6, the synergistic effect of ligusticum wallichii and cherry blossoms can improve dull aging such as skin pigmentation, wrinkles, and facial relaxation.

[0034] The encapsulation efficiency of the composite lipid nanoparticles prepared in Examples 1-3 and Comparative Examples 7-8 was measured, and the measurement results are shown in Table 2 for reference.

[0035] Table 2. Encapsulation efficiency measurement results of Examples 1-3 and Comparative Examples 7-8 Entrapment efficiency (%) Example 1 95.2 Example 2 95.5 Example 3 94.9 Comparative Example 7 88.3 Comparative Example 8 87.2 As can be seen from the data in Table 2, the encapsulation efficiency of Examples 1-3 of the present invention is significantly higher than that of Comparative Examples 7-8, indicating that the addition of tea saponin and sophorolipid can improve the encapsulation ability of the inclusion complex for the compound plant essential oil, reduce the leakage of the compound plant essential oil, and improve the stability.

[0036] The above examples are only illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Any person familiar with this technology can modify or change the above examples without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or changes made by those with ordinary knowledge in the technical field without departing from the spirit and technical ideas disclosed by the present invention should still be covered by the claims of the present invention.

Claims

1. A preparation method of a compound essential oil with anti-aging efficacy, characterized in that, It includes the following steps: S1: Preparation of compound plant essential oil Taking pomegranate seeds, edelweiss, cherry blossoms, chuanxiong rhizome, perilla and immortelle as raw materials to extract compound plant essential oil; S2: Preparation of compound lipid nanocapsules Adding lecithin, tea saponin and sophorolipid into deionized water to obtain a mixed solution, mixing glyceryl distearate, caprylic / capric triglyceride and compound plant essential oil to obtain an oil-phase solution, and mixing and stirring the oil-phase solution and the mixed solution, followed by ultrasonic curing to obtain compound lipid nanocapsules; S3: Preparation of compound essential oil Mixing phospholipids, glycerol, hydroxyethyl cellulose, polyglyceryl monolaurate and trehalose to obtain mixture Ⅰ, mixing an alcohol solvent, olive oil, jojoba oil, frankincense oil and compound lipid nanocapsules, and then adding them to mixture Ⅰ to obtain mixture Ⅱ, adding deionized water to mixture Ⅱ, and stirring and mixing to obtain compound essential oil.

2. The preparation method of a compound essential oil with anti-aging efficacy according to claim 1, characterized in that, For the preparation of compound plant essential oil in step S1, it specifically includes the following steps: S1.1: Weigh pomegranate seeds, edelweiss, cherry blossoms, chuanxiong rhizome, perilla and immortelle respectively and mix them, perform freeze-drying treatment at a temperature of -20°C to -25°C for 15 - 20 h, and then perform low-temperature ultrafine grinding treatment at -8°C to -10°C for 15 - 20 min to obtain compound plant powder; S1.2: Place the compound plant powder in a round-bottom flask, add 10 - 12 times the amount of water, add cellulase to make the final concentration of cellulase 1 - 2%, then adjust the pH to 4.5 - 5, heat to 40 - 45°C, enzymatically hydrolyze for 30 - 40 min, then connect the distillation device and start distilling for 2 - 3 h, and collect the essential oil with an oil-water separator to obtain compound plant essential oil.

3. The preparation method of a compound essential oil with anti-aging effect according to claim 2, characterized in that, For the preparation of compound lipid nanocapsules in step S2, it specifically includes the following steps: S2.1: Mix lecithin, tea saponin and sophorolipid and add them to deionized water, stir and mix for 20 - 30 min to obtain a mixed solution; S2.2: Mix 1 - 3 parts by weight of glyceryl distearate and 1 - 3 parts by weight of caprylic / capric triglyceride at 73 - 75°C, and then add 3 - 5 parts by weight of compound plant essential oil, and stir and mix at 200 - 300 rpm to obtain an oil-phase solution; S2.3: Under the condition of magnetic stirring at 1000 - 1200 r / min, drop the oil-phase solution into the mixed solution at 73 - 75°C, stir to form a primary emulsion, put the primary emulsion into an ultrasonic cell disruptor for ultrasonic dispersion, cool and solidify in an ice-water bath for 30 - 40 min, and then filter through a 0.45 μm microporous membrane to obtain compound lipid nanocapsules.

4. The preparation method of a compound essential oil with anti-aging effect according to claim 3, characterized in that, For the preparation of compound essential oil in step S3, it specifically includes the following steps: S3.1: Mix 3 - 5 parts by weight of phospholipids, 1 - 3 parts by weight of glycerol, 1 - 3 parts by weight of hydroxyethyl cellulose, 3 - 9 parts by weight of polyglyceryl monolaurate and 1 - 2 parts by weight of trehalose, heat to 65 - 70°C, and stir at 500 - 800 r / min for 8 - 10 min to obtain mixture Ⅰ; S3.2: After mixing 2-4 parts by weight of an alcohol solvent, 1-3 parts by weight of olive oil, 2-3 parts by weight of jojoba oil, 0.1-0.3 parts by weight of frankincense oil, and 25-30 parts by weight of a composite lipid nano-inclusion, add them to Mixture I, and stir evenly to obtain Mixture II; S3.3: Add 30-50 parts by weight of deionized water at 65-70 °C to Mixture II, and then continue to stir at 20000-25000 r / min for 10-15 min to obtain a compound essential oil.

5. The preparation method of a compound essential oil with anti-aging efficacy according to claim 2, characterized in that, In step S1.1, the weight ratio of pomegranate seeds, leontopodium alpinum, cherry blossoms, ligusticum wallichii, perilla, and immortelle is (2-3):(1-2):(1-3):(2-3):(3-5):(2-3).

6. The preparation method of a compound essential oil with anti-aging efficacy according to claim 3, characterized in that, In the mixed solution of step S2.1, the mass fraction of lecithin is 2-3%, the mass fraction of tea saponin is 0.3-0.5%, and the mass fraction of sophorolipid is 0.2-0.3%.

7. The preparation method of a compound essential oil with anti-aging effect according to claim 3, characterized in that, In step S2.3, the volume ratio of the oil phase solution to the mixed solution is 1:8-10.

8. The preparation method of a compound essential oil with anti-aging effect according to claim 4, characterized in that, In step S3.2, the alcohol solvent is propylene glycol.

9. A compound essential oil with anti-aging efficacy, characterized in that, It is prepared by the preparation method of a compound essential oil with anti-aging efficacy according to any one of claims 1-8.

Images