Efficient water locking and repairing mask and preparation method thereof

Abstract

The invention discloses an efficient water-locking repair mask and a preparation method thereof, and belongs to the technical field of cosmetics, the efficient water-locking repair mask comprises the following components by mass: 0.5-2.0% of an astragalus membranacus extract fermentation broth, 0.1-0.5% of ceramide NP, 0.05-0.3% of a sodium hyaluronate composition, 0.1-0.3% of polyquaternium-51, 1-3% of a lactobacillus plantarum fermentation lysate, 1-5% of ethanol, and the balance of deionized water. The lactobacillus plantarum fermentation lysate comprises propionic acid and butyric acid. The Astragalus membranacus extract fermentation broth can remove allergic components and form a lipid-polysaccharide interpenetrating network with ceramide NP, and the Astragalus membranacus extract fermentation broth and ceramide NP synergistically simulate a natural barrier structure of skin, so that the order degree of cuticle lipid is improved, transdermal moisture loss is prevented, and the repairing and water locking functions are remarkably enhanced; butyric acid and propionic acid in the lactobacillus plantarum fermentation lysate promote penetration of ceramide NP by acidifying a cuticle microenvironment (pH 4.5), and the biological safety is high.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to an efficient water-locking and repairing facial mask and a preparation method thereof. Background Art

[0002] With the research and development of cosmetics science and the increasing demand of consumers for the efficacy of cosmetics, repairing damaged skin barriers and enhancing the resistance of the skin have become one of the main demands of the majority of consumers. Most of the traditional barrier-repairing facial masks on the market rely on single moisturizing ingredients (such as hyaluronic acid, ceramide NP), and cannot simulate the "brick wall structure" of the skin, resulting in low repair efficiency.

[0003] Moreover, conventional preparation processes (such as high-temperature emulsification) are prone to disadvantages such as polysaccharide degradation and lipid oxidation. Due to the difficulty of realizing the staged release of components in the prior art, there is a phenomenon that macromolecular components remain in the epidermis and small molecules have insufficient penetration; most facial masks on the market add synthetic thickeners (such as carbomer) and silicone oil matrices, resulting in a biodegradation rate of <40% (OECD301B), which does not conform to the trend of green cosmetics.

[0004] In view of the problems existing in the above-mentioned prior art, the present invention combines the design and use experience in related fields for many years, and designs an efficient water-locking and repairing facial mask and a preparation method thereof to overcome the above defects. Summary of the Invention

[0005] For the problems existing in the prior art, an efficient water-locking and repairing facial mask and a preparation method thereof provided by the present invention can simulate the lipid bilayer structure of the stratum corneum, enhance the ordered arrangement of intercellular lipids (ceramide NP, cholesterol), reduce transepidermal water loss (TEWL), improve the transdermal efficiency, and maintain the water content of the stratum corneum for a long time, meeting the requirements of medical-grade precise repair and daily protection.

[0006] To achieve the above object, in the first aspect of the present invention, an efficient water-locking and repairing facial mask is provided, which comprises the following components in mass percentages: Astragalus membranaceus extract fermentation broth 0.5-2.0%, ceramide NP 0.1-0.5%, sodium hyaluronate composition 0.05-0.3%, polyquaternium-51 0.1-0.3%, Lactobacillus plantarum fermentation lysate 1-3%, ethanol 1-5%, and the balance is deionized water; The Lactobacillus plantarum fermentation lysate includes propionic acid and butyric acid; The preparation method of the Astragalus membranaceus extract fermentation broth comprises the following steps: (1) Mix 5-15 g of Astragalus membranaceus extract with Aspergillus niger strain to obtain a mixture, and the inoculation amount of the Aspergillus niger strain is 5-10% of the mass of the Astragalus membranaceus extract; (2)Prepare a solid fermentation medium, which includes the following components by mass fraction: wheat bran 2-5%, potassium dihydrogen phosphate 0.1-0.3%, magnesium sulfate 0.02-0.07%, and the balance is water; (3)Add the mixture from step (1) to the solid fermentation medium in step (2) and place it in a fermentation container. Ferment at 20-40°C and pH = 5-7 for 50-70 hours to obtain a fermentation product. Stir for 1-10 minutes at 20-40 rpm every 5-7 hours during fermentation; (4)Subject the fermentation product to ultrasonic treatment, centrifugation, and ultrafiltration to obtain the Astragalus membranaceus extract fermentation broth; The mass ratio of the mixture in step (1) to the solid fermentation medium in step (2) is 1:1.5; The Aspergillus niger strain is Aspergillus niger ( Aspergillus niger ) CICC 2487, purchased from the China Center of Industrial Culture Collection (CICC).

[0007] Preferably, in step (4), the fermentation product is ultrasonically extracted at a frequency of 30-50 kHz and 40-60°C for 15-20 min; In step (4), ultrafiltration treatment is carried out using an ultrafiltration tube, and the cut-off molecular weight of the ultrafiltration tube is 5-50 KDa.

[0008] Preferably, the mass ratio of the ceramide NP to the Astragalus membranaceus extract fermentation broth is (1:2)-(1:5).

[0009] Preferably, the sodium hyaluronate composition is composed of sodium hyaluronate with molecular weights of 1 kDa, 100 kDa, and 1 MDa in a mass ratio of 1:2:1.

[0010] Preferably, the preparation method of the Lactobacillus plantarum fermentation lysate includes the following steps: S1. Add the Astragalus membranaceus extract and Lactobacillus plantarum to the MRS liquid medium and ferment at 30-40°C for 40-60 hours to obtain a Lactobacillus plantarum fermentation product. The Lactobacillus plantarum is Lactobacillus plantarum ( Lactobacillus plantarum ) CICC 25125, purchased from the China Center of Industrial Culture Collection; S2. Centrifuge and filter the Lactobacillus plantarum fermentation product to obtain the Lactobacillus plantarum fermentation lysate; The mass content of propionic acid in the Lactobacillus plantarum fermentation lysate is 0.5-1.5%, and the mass content of butyric acid is 0.3-1.2%; The mass ratio of the Astragalus membranaceus extract to the volume of the MRS liquid medium is (5-15%):1; The mass ratio of the Lactobacillus plantarum to the MRS liquid medium is (5 - 10%):1; In step S2, sterile filtration is carried out using a 0.22 μm microporous membrane.

[0011] The present invention also provides a method for preparing an efficient water - locking repair facial mask, comprising the following steps: A1. Premix the Astragalus membranaceus extract fermentation broth, sodium hyaluronate composition, polyquaternium - 51, Lactobacillus plantarum fermentation lysate and deionized water to obtain a premixed solution; A2. Introduce the premixed solution into a Y - type microfluidic chip to form a uniform colloid; A3. Dissolve ceramide NP in ethanol, add it to the colloid obtained in step A2, perform ultrasonic treatment at 40 - 50 °C for 5 - 15 minutes at 30 - 50 kHz, and then carry out penetration at a negative pressure of - 0.05~ - 1 MPa for 10 - 20 minutes to obtain a colloid loaded with ceramide NP; A4. Inject the colloid loaded with ceramide NP into a silica gel microporous mold, perform vacuum freeze - drying at - 20 °C for 1 - 3 hours until the moisture content ≤ 10%, package and sterilize by γ - ray irradiation to obtain the efficient water - locking repair facial mask; The mass ratio of the ceramide NP to ethanol is 1:10; The pore diameter of the silica gel microporous mold is 100 μm, and the dose of the γ - ray is 20 - 30 kGy; The system temperature of the Y - type microfluidic chip is 5 - 10 °C, and the pressure is 30 - 60 MPa.

[0012] Preferably, the Y - type microfluidic chip comprises a main channel and two branch channels, the main channel and the two branch channels are interconnected, and the included angle between the two branch channels is 10 - 150°; The diameter of the main channel is 500 μm, and the flow rate of the main channel is 0.5 - 2 mL / min; The diameter of the branch channel is 200 μm, and the flow rate of the branch channel is 0.25 - 1 mL / min.

[0013] The advantages of this invention are as follows: 1. The present invention uses a compound of the fermentation broth of Astragalus membranaceus extract and ceramide NP. After fermentation, the Astragalus membranaceus extract can effectively remove irritating components, such as allergenic saponins, retain high-molecular-weight active components, and increase the content of β-1,4-glucan, with the content of β-1,4-glucan reaching more than 56.1%. β-1,4-glucan can enhance the ordered arrangement of intercellular lipids (such as ceramide NP and cholesterol) by mimicking the lipid bilayer structure of the stratum corneum, reduce transepidermal water loss (TEWL), form a hydrogen bond network between the hydroxyl groups on the molecular chain and water molecules, and maintain the water content of the stratum corneum for a long time. Its linear chain structure can be used as a carrier to load ceramide NP, sodium hyaluronate composition and polyquaternium-51, and form a "lipid-polysaccharide interpenetrating network" with ceramide NP through electrostatic self-assembly. The two cooperate to mimic the natural skin barrier structure, improve the order degree of stratum corneum lipids, prevent transepidermal water loss, and significantly enhance the repair and water-locking function.

[0014] 2. In the present invention, butyric acid and propionic acid in the fermentation lysate of Lactobacillus plantarum promote the penetration of ceramide NP by acidifying the stratum corneum microenvironment (pH 4.5). The fermentation lysate of Lactobacillus plantarum simultaneously realizes the synergistic improvement of the transdermal efficiency of active components, barrier repair and microecological regulation, while exogenous acids only have a single antibacterial function. The fermentation lysate of Lactobacillus plantarum comprehensively surpasses exogenous butyric acid / propionic acid in terms of efficacy, safety and sustainability through the composite effect of natural components, precise microecological regulation and process compatibility optimization.

[0015] 3. In the present invention, a low-temperature (i.e., 5-10°C) microfluidic process is adopted, combined with a Y-shaped channel. Through precise shear force regulation and low-temperature protection, a uniform nanoscale colloidal structure is formed, providing a good carrier and laying the foundation for a high encapsulation rate. By optimizing the mass ratio of ceramide NP to the fermentation broth of Astragalus membranaceus extract, the intermolecular force between lipid and polysaccharide molecules is maximized, avoiding encapsulation failure caused by the separation of the "lipid-polysaccharide interpenetrating network". Through the synergistic treatment of ultrasound and negative pressure, the penetration of ceramide NP into the colloid is enhanced, and the encapsulation rate of ceramide NP is increased, making the encapsulation rate of ceramide NP greater than or equal to 78.4%. The low-temperature (5-10°C) microfluidic process combined with the freeze-drying process is used to inhibit the degradation of β-1,4-glucan, so that the retention rate of β-1,4-glucan is greater than or equal to 89.7%, enabling β-1,4-glucan in the highly efficient water-locking repair facial mask to fully play its role. Description of the Drawings

[0016] Figure 1 It is a schematic diagram of a Y-shaped microfluidic chip.

[0017] In the figure: 1 - branch channel, 2 - main channel. Detailed Embodiments

[0018] For the convenience of those skilled in the art to understand, the present invention will be further described below in conjunction with specific embodiments.

[0019] The first aspect of the present invention provides an efficient water-locking and repairing facial mask, which comprises the following components in mass fractions: fermented liquid of Astragalus membranaceus extract 0.5 - 2.0%, ceramide NP 0.1 - 0.5%, sodium hyaluronate composition 0.05 - 0.3%, polyquaternium-51 0.1 - 0.3%, fermented lysate of Lactobacillus plantarum 1 - 3%, ethanol 1 - 5%, and deionized water as the balance. The fermented lysate of Lactobacillus plantarum includes propionic acid and butyric acid. The mass ratio of ceramide NP to the fermented liquid of Astragalus membranaceus extract is (1:2) - (1:5), and the sodium hyaluronate composition is composed of sodium hyaluronate with molecular weights of 1 kDa, 100 kDa, and 1 MDa in a mass ratio of 1:2:1.

[0020] The present invention uses the fermented liquid of Astragalus membranaceus extract and ceramide NP for compounding. After fermentation, the Astragalus membranaceus extract can remove some impurities, including the irritating component sensitizing saponin, improve biocompatibility, reduce skin allergies, and at the same time retain the high-molecular-weight active ingredient β-1,4-glucan, which can reduce the damage of free radicals, alleviate the inflammatory response, and enhance the immunity of the skin. And after fermentation, the mass content of β-1,4-glucan reaches more than 56.1%. β-1,4-glucan can simulate the lipid bilayer structure of the stratum corneum, enhance the ordered arrangement of intercellular lipids (ceramide NP, cholesterol), reduce transepidermal water loss (TEWL), and the hydroxyl groups on the molecular chain form a hydrogen bond network with water molecules, which can maintain the water content of the stratum corneum for a long time; it also forms a "lipid-polysaccharide interpenetrating network" with ceramide NP through electrostatic self-assembly. The two work together to simulate the natural skin barrier structure, improve the order degree of the stratum corneum lipids, significantly enhance the water-locking and repairing function, and assist and strengthen the long-term water-locking ability through sodium hyaluronate with different molecular weights and polyquaternium-51.

[0021] The linear chain structure of β-1,4-glucan also serves as a carrier to load active ingredients such as ceramide NP, sodium hyaluronate composition, and polyquaternium-51, which is beneficial to the penetration of active ingredients. Butyric acid and propionic acid in the fermented lysate of Lactobacillus plantarum promote the penetration of ceramide NP into the skin by acidifying the microenvironment of the stratum corneum (pH 4.5), synergistically improving the transdermal efficiency of active ingredients. Butyric acid and propionic acid can also activate filaggrin-degrading enzymes to accelerate the generation of natural moisturizing factors (NMF). Sodium hyaluronate compositions with different molecular weights can moisturize the skin at different depths and prevent water loss, and achieve long-term water replenishment under the action of the β-1,4-glucan carrier. Polyquaternium-51 has strong hygroscopicity and synergistically improves the water-locking ability of the "lipid-polysaccharide interpenetrating network".

[0022] The preparation method of the fermented liquid of Astragalus membranaceus extract in the present invention includes the following steps: (1) Mix 5 - 15 g of Astragalus membranaceus extract with Aspergillus niger strain with the preservation number of CICC 2487 ( Aspergillus niger ), to obtain a mixture, wherein the inoculation amount of the Aspergillus niger strain is 5 - 10% of the mass of the Astragalus membranaceus extract; (2) Prepare a solid fermentation medium, which includes the following components by mass fraction: wheat bran 2 - 5%, potassium dihydrogen phosphate 0.1 - 0.3%, magnesium sulfate 0.02 - 0.07%, and the balance is water; (3) Add the mixture from step (1) to the solid fermentation medium in step (2) and place it in a fermentation container. Ferment at 20 - 40 °C and pH = 5 - 7 for 50 - 70 hours to obtain a fermentation product. During fermentation, at intervals of 5 - 7 hours, start the stirring device to run at a speed of 20 - 40 rpm for 1 - 10 minutes, and then let it stand until the next stirring cycle, wherein the mass ratio of the mixture in step (1) to the solid fermentation medium in step (2) is 1:1.5; (4) Place the fermentation product in an ultrasonic extraction device, perform ultrasonic-assisted extraction at 30 - 50 kHz and 40 - 60 °C for 15 - 20 minutes, then centrifuge at a speed of 6000 - 8000 rpm for 20 - 30 minutes, collect the first supernatant, and fractionate the first supernatant through an ultrafiltration tube to retain the fraction with a molecular weight cut-off of 5 - 50 kDa to obtain the Astragalus membranaceus extract fermentation broth.

[0023] The preparation method of the Lactobacillus plantarum fermentation lysate in the present invention includes the following steps: S1. Add Astragalus membranaceus extract and Lactobacillus plantarum with the preservation number of CICC 25125 to the MRS liquid medium ( Lactobacillus plantarum ), and ferment at 30 - 40 °C for 40 - 60 hours to obtain a Lactobacillus plantarum fermentation product; S2. Centrifuge the Lactobacillus plantarum fermentation product at 5000 - 6000 rpm for 8 - 12 minutes, collect the second supernatant, and perform sterile filtration of the second supernatant through a 0.22 μm pore size filter membrane. The collected filtrate is the Lactobacillus plantarum fermentation lysate.

[0024] Wherein the mass (gram) ratio of the Astragalus membranaceus extract to the volume (milliliter) of the MRS liquid medium is (5 - 15%):1, the mass ratio of the Lactobacillus plantarum to the MRS liquid medium is (5 - 10%):1, and the MRS liquid medium is a commonly used fermentation medium in the art. The Lactobacillus plantarum fermentation lysate includes propionic acid with a mass fraction of 0.5 - 1.5% and butyric acid with a mass fraction of 0.3 - 1.2%.

[0025] Butyric acid and propionic acid can promote moisturizing. However, directly adding propionic acid and butyric acid to the facial mask may lead to microecological imbalance and interfere with the hydration ability of sodium hyaluronate. In the present invention, Lactobacillus plantarum is used to ferment the extract of Astragalus membranaceus. During fermentation, precise microecological regulation and optimization of process compatibility are carried out to obtain a Lactobacillus plantarum fermentation lysate with excellent efficacy, safety and sustainability.

[0026] The present invention also provides a method for preparing a highly efficient water-locking and repairing facial mask, comprising the following steps: A1. Premix the fermentation broth of Astragalus membranaceus extract, sodium hyaluronate composition, polyquaternium-51, Lactobacillus plantarum fermentation lysate and deionized water to obtain a premixed solution; A2. Introduce the premixed solution into a Y-shaped microfluidic chip, and control the system temperature at 5-10 °C and the pressure at 30-60 MPa through a circulating cooling system. The premixed solution is uniformly mixed in the Y-shaped microfluidic chip to form a homogeneous colloid; A3. Dissolve ceramide NP in ethanol, add the above colloid, and perform ultrasonic treatment at 30-50 Hz and 40-50 °C for 5-15 minutes, and then permeate at a negative pressure of -0.05 to -1 MPa for 10-20 minutes to enable ceramide NP to be directionally embedded in the colloid network, obtaining a colloid loaded with ceramide NP, wherein the mass ratio of ceramide NP to ethanol is 1:10; A4. Inject the colloid loaded with ceramide NP into a silica microporous mold with a pore size of 100 μm, perform vacuum freeze-drying at -20 °C for 1-3 hours until the water content is ≤10%, then carry out sub-packaging, and after sub-packaging, sterilize with γ-ray irradiation to obtain a highly efficient water-locking and repairing facial mask, wherein the γ-ray dose is 20-30 kGy; In the present invention, the Y-shaped microfluidic chip and the circulating cooling system are both conventional devices, such as Figure 1As shown in the figure. The Y-shaped microfluidic chip includes a main channel 2 and two branch channels 1. The main channel 2 and the two branch channels 1 are interconnected. The included angle between the two branch channels 1 is 10 - 150°. Both the main channel 2 and the branch channels 1 are circular. The premixed liquid enters from the two branch channels 1, converges in the main channel 2 and flows out from the main channel. The diameter of the main channel 2 is 500 μm, and the flow rate of the main channel 2 is 0.5 - 2 mL / min; the diameter of the branch channel 1 is 200 μm, and the flow rate of the branch channel 1 is 0.25 - 1 mL / min. When the high-efficiency water-locking repair facial mask of the present invention is used, it is hydrated and activated by spraying sterile deionized water or physiological saline (0.5 - 1.0 mL / film) on the dry film for 20 - 30 seconds. The hydrated film is gently applied to the facial skin after cleaning, pressed and adjusted according to the facial contour, and is adhesively attached without glue by using its bionic adhesiveness to avoid mechanical friction and irritation. Then it is left standing for 15 - 20 minutes. During this period, the complex (ceramide NP-β-1,4-glucan) continuously releases active ingredients. The film is gently removed by peeling it off from bottom to top along the facial contour without secondary cleaning. The residual gel can be gently patted until absorbed, and its self-ablation property ensures that there is no residue of the film cloth. Finally, the subsequent skin care procedures (essence / cream) are directly carried out, and the bionic lipid layer formed by the film can enhance the product penetration.

[0027] The present invention adopts a low-temperature (5 - 10°C) microfluidic process, combined with a Y-shaped channel. Through the precise shear force regulation and low-temperature protection in the microfluidic process, a uniform nano-scale carrier structure is formed, laying the foundation for a high encapsulation rate; then through the synergistic treatment of ultrasound-negative pressure, the penetration of ceramide NP into the colloid and the adaptability of the colloid pores are strengthened, and the encapsulation rate of ceramide NP is increased. The present invention also continuously optimizes the mass ratio of ceramide NP to the fermentation broth of Astragalus membranaceus extract to ensure the maximization of the intermolecular force between lipid-polysaccharide molecules, so that the encapsulation rate of ceramide NP is greater than or equal to 78.4%. In the present invention, the low-temperature (5 - 10°C) microfluidic process and the vacuum freeze-drying process inhibit the degradation of β-1,4-glucan and the oxidation of lipids, so that the molecular weight retention rate of β-1,4-glucan is greater than or equal to 89.7%, and a larger "lipid-polysaccharide interpenetrating network" can be constructed. The high-efficiency water-locking repair facial mask of the present invention is suitable for skin barrier damage (such as atopic dermatitis, after laser surgery), sensitive skin and environmental pollution exposure scenarios, meeting the medical-grade precise repair and daily protection needs.

[0028] The specific embodiments are as follows The experimental methods used in the present invention are all conventional methods without special instructions. The materials, reagents, etc. used can be obtained from commercial channels without special instructions. The Aspergillus niger strain with the preservation number CICC 2487 ( Aspergillus niger ) and the Lactobacillus plantarum with the preservation number CICC 25125 ( Lactobacillus plantarum) All were purchased from the China Center for Industrial Culture Collection.

[0029] Among them, the Astragalus membranaceus extract was purchased from Shandong Dongfang Hongye Chemical Co., Ltd.; Test method and standard for the content of β-1,4-glucan in the Astragalus membranaceus extract fermentation broth in Preparation Examples 1-3: Standard reference: SN / T 4260-2015 Determination of crude polysaccharides in exported plant-derived foods Detection method: Phenol-sulfuric acid colorimetry Detection steps: (1) Sample pretreatment: Centrifugation to remove residues: Take the Astragalus membranaceus extract fermentation broth and centrifuge it at 10000 rpm for 15 min to collect the supernatant; (2) Protein removal: Use the Sevag method (Sevage reagent is chloroform: n-butanol = 4:1, v / v) to shake and remove protein from the above supernatant 3 times, 10 min each time, to remove free protein; (3) Dialysis purification: Dialyze using a dialysis bag (Spectra / Por®) with a molecular weight cut-off of 3.5 kDa for 48 h, and freeze-dry to obtain purified polysaccharide; (4) Color reaction: Dissolve the freeze-dried purified polysaccharide in ultrapure water to prepare a test solution of 0.5 mg / mL (need to vortex until completely dissolved); Take 0.5 mL of the test solution into a 10 mL stoppered colorimetric tube, add 1 mL of 5% (w / w) phenol aqueous solution, quickly add 5 mL of concentrated sulfuric acid, mix well and let stand for 30 min, and measure the absorbance at 490 nm (UV-Vis spectrophotometer).

[0030] Standard curve: Using glucose (CAS 50-99-7) as the standard product, prepare a series of solutions of 0, 20, 40, 60, 80, 100 μg / mL, and establish a linear equation (R²≥0.999).

[0031] Preparation Example 1 - Astragalus membranaceus extract fermentation broth This preparation example provides a preparation method for an Astragalus membranaceus extract fermentation broth, including the following steps: (1) Mix 10 g of the Astragalus membranaceus extract with the Aspergillus niger strain to obtain a mixture, and the inoculation amount of Aspergillus niger (preservation number CICC2487) is 5% of the mass of the Astragalus membranaceus extract; (2) Prepare 15.75 g of a solid fermentation medium. The solid fermentation medium includes the following components by mass fraction: wheat bran 3%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.05%, and the balance is water; (3) Add the mixture from step (1) to the solid fermentation medium in step (2) and place it in a fermentation vessel. Ferment at 30 °C and pH = 5.5 for 60 hours to obtain a fermentation product. Stir at a speed of 30 rpm for 5 minutes every 6 hours during fermentation; (4) Ultrasonically extract the fermentation product at 40 kHz and 50 °C for 15 minutes, then centrifuge at a speed of 8000 rpm for 20 minutes, and collect the first supernatant; Subject the first supernatant to molecular weight fractionation through an ultrafiltration tube, and retain the fraction with a molecular weight cut-off of 5 - 50 kDa to obtain the Astragalus membranaceus extract fermentation broth.

[0032] In this preparation example, the mass content of β-1,4-glucan in the Astragalus membranaceus extract fermentation broth is 68.3 ± 1.5%.

[0033] Preparation Example 2 - Astragalus membranaceus extract fermentation broth This preparation example provides a method for preparing an Astragalus membranaceus extract fermentation broth, which includes the following steps: (1) Mix 5 g of Astragalus membranaceus extract with Aspergillus niger strain to obtain a mixture. The inoculation amount of Aspergillus niger (preservation number CICC2487) is 8% of the mass of Astragalus membranaceus extract; (2) Prepare 8.1 g of solid fermentation medium. The solid fermentation medium includes the following components by mass fraction: wheat bran 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.02%, and the balance is water; (3) Add the mixture from step (1) to the solid fermentation medium in step (2) and place it in a fermentation vessel. Ferment at 20 °C and pH = 5 for 50 hours to obtain a fermentation product. Stir at a speed of 20 rpm for 1 minute every 5 hours during fermentation; (4) Ultrasonically extract the fermentation product at 30 kHz and 40 °C for 18 minutes, then centrifuge at a speed of 6000 rpm for 25 minutes, and collect the first supernatant; Subject the first supernatant to molecular weight fractionation through an ultrafiltration tube, and retain the fraction with a molecular weight cut-off of 5 - 50 kDa to obtain the Astragalus membranaceus extract fermentation broth.

[0034] In this preparation, the mass content of β-1,4-glucan in the Astragalus membranaceus extract fermentation broth is 56.1 ± 1.2%.

[0035] Preparation Example 3 - Astragalus membranaceus extract fermentation broth This preparation example provides a method for preparing an Astragalus membranaceus extract fermentation broth, which includes the following steps: (1) Mix 15 g of Astragalus membranaceus extract with Aspergillus niger strain to obtain a mixture. The inoculation amount of Aspergillus niger (preservation number CICC2487) is 10% of the mass of Astragalus membranaceus extract; (2) Prepare 24.75 g of solid fermentation medium. The solid fermentation medium includes the following components by mass fraction: wheat bran 5%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.07%, and the balance is water; (3) Add the mixture from step (1) to the solid fermentation medium in step (2) and place it in a fermentation container. Ferment at 40 °C and pH = 7 for 70 hours to obtain a fermentation product. During fermentation, stir at a speed of 40 rpm for 10 minutes every 7 hours; (4) Ultrasonically extract the fermentation product at 50 kHz and 60 °C for 20 minutes, then centrifuge at a speed of 5500 rpm for 30 minutes, and collect the first supernatant; Subject the first supernatant to molecular weight fractionation through an ultrafiltration tube, and retain the fraction with a molecular weight cut-off of 5 - 50 kDa to obtain the Astragalus membranaceus extract fermentation broth.

[0036] In this preparation, the mass content of β-1,4-glucan in the Astragalus membranaceus extract fermentation broth is 59.5 ± 1.1%.

[0037] Detection method and reference standard for the contents of butyric acid and propionic acid in the fermentation lysate of Lactobacillus plantarum in Preparation Examples 4 - 6: Test method: Quantitative determination of organic acids by high performance liquid chromatography Reference standard: Retention time of butyric acid: about 8.5 min, propionic acid: about 6.2 min (refer to General Principles 0512, Volume IV of Chinese Pharmacopoeia 2020 Edition).

[0038] Sample treatment: After centrifuging and filtering the fermentation lysate of Lactobacillus plantarum, take 1.00 mL of the filtrate and dilute it 10 times with ultrapure water. Pass it through a C18 solid phase extraction column to remove pigments and macromolecular interfering substances.

[0039] Chromatographic conditions: Chromatographic column: C18 column (250 mm × 4.6 mm, 5 μm); Mobile phase: 0.01 M potassium dihydrogen phosphate (pH 2.5) - acetonitrile (95:5, v / v); Flow rate: 1.0 mL / min, column temperature: 30 °C, injection volume 10 μL; Detection wavelength: 210 nm (ultraviolet detection).

[0040] Preparation Example 4 - Fermentation lysate of Lactobacillus plantarum The preparation method of the fermentation lysate of Lactobacillus plantarum includes the following steps: S1. Add 5% (m / v) of Astragalus membranaceus extract and 5% (w / w) of Lactobacillus plantarum (preservation number: CICC 25125) to the MRS liquid medium, and ferment at 35 °C for 50 hours to obtain a fermentation product of Lactobacillus plantarum; S2. Centrifuge the fermentation product of Lactobacillus plantarum at 6000 rpm for 10 minutes, collect the second supernatant, filter the second supernatant aseptically through a filter membrane with a pore size of 0.22 μm, and collect the filtrate to obtain the fermentation lysate of Lactobacillus plantarum, with the mass content of propionic acid being 1% and the mass content of butyric acid being 0.8%.

[0041] Preparation Example 5 - Fermentation Lysate of Lactobacillus plantarum The preparation method of the fermentation lysate of Lactobacillus plantarum includes the following steps: S1. Add 10% (m / v) of Astragalus membranaceus extract and 10% (w / w) of Lactobacillus plantarum (preservation number: CICC 25125) to the MRS liquid medium, and ferment at 35 °C for 50 hours to obtain the fermentation product of Lactobacillus plantarum; S2. Centrifuge the fermentation product of Lactobacillus plantarum at 5000 rpm for 8 minutes, collect the second supernatant, filter the second supernatant aseptically through a filter membrane with a pore size of 0.22 μm, and collect the filtrate to obtain the fermentation lysate of Lactobacillus plantarum, with the mass content of propionic acid being 0.8% and the mass content of butyric acid being 0.5%.

[0042] Preparation Example 6 - Fermentation Lysate of Lactobacillus plantarum The preparation method of the fermentation lysate of Lactobacillus plantarum includes the following steps: S1. Add 7% (m / v) of Astragalus membranaceus extract and 7% (w / w) of Lactobacillus plantarum (preservation number: CICC 25125) to the MRS liquid medium, and ferment at 30 °C for 40 hours to obtain the fermentation product of Lactobacillus plantarum; S2. Centrifuge the fermentation product of Lactobacillus plantarum at 5500 rpm for 12 minutes, collect the second supernatant, filter the second supernatant aseptically through a filter membrane with a pore size of 0.22 μm, and collect the filtrate to obtain the fermentation lysate of Lactobacillus plantarum, with the mass content of propionic acid being 0.6% and the mass content of butyric acid being 0.3%.

[0043] In the following examples and comparative examples, the sodium hyaluronate composition is composed of sodium hyaluronate with molecular weights of 1 kDa, 100 kDa, and 1 MDa in a mass ratio of 1:2:1. Example 1

[0044] In this example, the fermentation broth of Astragalus membranaceus extract was prepared according to the preparation method of Preparation Example 1, and the fermentation lysate of Lactobacillus plantarum was prepared according to the preparation method of Preparation Example 4; This embodiment provides an efficient water-locking repair facial mask, which comprises the following raw materials by mass fraction: 1.5% Astragalus membranaceus extract fermentation broth (1.5 g), 0.5% ceramide NP (0.5 g), 0.2% sodium hyaluronate composition (0.2 g), 0.3% polyquaternium-51 (0.3 g), 2% Lactobacillus plantarum fermentation lysate (2 g), 90.5% deionized water (90.5 g), 5% ethanol (5 g).

[0045] This embodiment also provides a preparation method of the efficient water-locking repair facial mask, which comprises the following steps: A1. Premix the Astragalus membranaceus extract fermentation broth, sodium hyaluronate composition, polyquaternium-51, Lactobacillus plantarum fermentation lysate and deionized water to obtain a premixed solution; A2. Introduce the premixed solution into a Y-shaped microfluidic chip from two branch channels 1 (diameter 200 μm), and flow out from the main channel 2 (diameter 500 μm) to form a uniform colloid; The included angle between the two branch channels 1 is 60°, control the system temperature at 5 °C, the pressure at 40 MPa, the flow rate of the main channel 2 at 1 mL / min, and the flow rate of the branch channel 1 at 0.5 mL / min; A3. Dissolve ceramide NP in ethanol, add it to the colloid obtained in step A2, and after ultrasonic treatment at 40 kHz and 45 °C for 10 minutes, carry out penetration at a negative pressure of -0.8 MPa for 15 minutes to obtain a colloid loaded with ceramide NP; A4. Inject the colloid loaded with ceramide NP into a silica gel microporous mold (pore diameter 100 μm), carry out vacuum drying at -20 °C (pressure ≤ 10 Pa) for 2 hours until the moisture content ≤ 5%, carry out sub-packaging and sterilize by γ-ray irradiation (γ-ray dose is 25 kGy) to obtain the efficient water-locking repair facial mask. Example Two

[0046] In this embodiment, the Astragalus membranaceus extract fermentation broth is prepared according to the preparation method of Preparation Example 1, and the Lactobacillus plantarum fermentation lysate is prepared according to the preparation method of Preparation Example 4; This embodiment provides an efficient water-locking repair facial mask, which comprises the following raw materials by mass fraction: 0.5% Astragalus membranaceus extract fermentation broth (0.5 g), 0.1% ceramide NP (0.1 g), 0.2% sodium hyaluronate composition (0.2 g), 0.3% polyquaternium-51 (0.3 g), 2% Lactobacillus plantarum fermentation lysate (2 g), 95.9% deionized water (95.9 g), 1% ethanol (1 g).

[0047] This embodiment also provides a preparation method of the efficient water-locking repair facial mask, which comprises the following steps: A1. Premix the Astragalus membranaceus extract fermentation broth, sodium hyaluronate composition, polyquaternium-51, Lactobacillus plantarum fermentation lysate and deionized water to obtain a premixed solution; A2. Introduce the premixed solution into the Y-shaped microfluidic chip from two branch channels 1 (with a diameter of 200 μm) and flow out from the main channel 2 (with a diameter of 500 μm) to form a uniform colloid. The included angle between the two branch channels 1 is 60°. Control the system temperature at 5 °C, the pressure at 40 MPa, the flow rate of the main channel 2 at 1 mL / min, and the flow rate of the branch channel 1 at 0.5 mL / min. A3. Dissolve ceramide NP in ethanol, add it to the colloid obtained in step A2, and ultrasonically treat it at 40 kHz and 45 °C for 10 minutes, and then infiltrate it at a negative pressure of -0.08 MPa for 15 minutes to obtain a colloid loaded with ceramide NP. A4. Inject the colloid loaded with ceramide NP into a silica microporous mold (with a pore diameter of 100 μm), and vacuum dry it at -20 °C (pressure ≤ 10 Pa) for 2 hours until the moisture content ≤ 5%; subpackage and sterilize it by γ-ray irradiation (γ-ray dose is 25 kGy) to obtain a high-efficiency water-locking and repairing facial mask. Example 3

[0048] In this example, the fermented liquid of Astragalus membranaceus extract is prepared according to the preparation method of Preparation Example 1, and the fermented lysate of Lactobacillus plantarum is prepared according to the preparation method of Preparation Example 4. This example provides a high-efficiency water-locking and repairing facial mask, including the following raw materials by mass fraction: 1.0% fermented liquid of Astragalus membranaceus extract (1.0 g), 0.5% ceramide NP (0.5 g), 0.2% sodium hyaluronate composition (0.2 g), 0.3% polyquaternium-51 (0.3 g), 2% fermented lysate of Lactobacillus plantarum (2 g), 91% deionized water (91 g), 5% ethanol (5 g).

[0049] This example also provides a preparation method of a high-efficiency water-locking and repairing facial mask, including the following steps: A1. Premix the fermented liquid of Astragalus membranaceus extract, sodium hyaluronate composition, polyquaternium-51, fermented lysate of Lactobacillus plantarum and deionized water to obtain a premixed solution. A2. Introduce the premixed solution into the Y-shaped microfluidic chip from two branch channels 1 (with a diameter of 200 μm) and flow out from the main channel 2 (with a diameter of 500 μm) to form a uniform colloid. The included angle between the two branch channels 1 is 60°. Control the system temperature at 5 °C, the pressure at 40 MPa, the flow rate of the main channel 2 at 1 mL / min, and the flow rate of the branch channel 1 at 0.5 mL / min. A3. Dissolve ceramide NP in ethanol, add it to the colloid obtained in step A2, and ultrasonically treat it at 40 kHz and 45 °C for 10 minutes, and then infiltrate it at a negative pressure of -0.08 MPa for 15 minutes to obtain a colloid loaded with ceramide NP. A4. Inject the colloidal solution loaded with ceramide NP into a silica microporous mold (pore diameter 100 μm), and dry it under vacuum at -20°C (pressure ≤ 10 Pa) for 2 hours until the moisture content ≤ 5%; divide it into packages and sterilize it by γ-ray irradiation (γ-ray dose is 25 kGy) to obtain a highly efficient water-locking and repairing facial mask. Example 4

[0050] In this example, the fermented liquid of Astragalus membranaceus extract is prepared according to the preparation method of Preparation Example 1, and the fermented and lysed liquid of Lactobacillus plantarum is prepared according to the preparation method of Preparation Example 4. This example provides a highly efficient water-locking and repairing facial mask, which includes the following raw materials by mass fraction: 2% fermented liquid of Astragalus membranaceus extract (2.0 g), 0.5% ceramide NP (0.5 g), 0.2% sodium hyaluronate composition (0.2 g), 0.3% polyquaternium-51 (0.3 g), 2% fermented and lysed liquid of Lactobacillus plantarum (2 g), 90% deionized water (90 g), 5% ethanol (5 g).

[0051] This example also provides a preparation method of a highly efficient water-locking and repairing facial mask, which includes the following steps: A1. Premix the fermented liquid of Astragalus membranaceus extract, sodium hyaluronate composition, polyquaternium-51, fermented and lysed liquid of Lactobacillus plantarum and deionized water to obtain a premixed solution. A2. Introduce the premixed solution into a Y-shaped microfluidic chip from two branch channels 1 (diameter 200 μm) and flow out from the main channel 2 (diameter 500 μm) to form a uniform colloid. The included angle between the two branch channels 1 is 60°, control the system temperature at 5°C, the pressure at 40 MPa, the flow rate of the main channel 2 at 1 mL / min, and the flow rate of the branch channel 1 at 0.5 mL / min. A3. Dissolve ceramide NP in ethanol, add it to the colloid obtained in step A2, ultrasonic treat it at 40 kHz and 45°C for 10 minutes, and then permeate it at a negative pressure of -0.08 MPa for 15 minutes to obtain a colloid loaded with ceramide NP. A4. Inject the colloidal solution loaded with ceramide NP into a silica microporous mold (pore diameter 100 μm), and dry it under vacuum at -20°C (pressure ≤ 10 Pa) for 2 hours until the moisture content ≤ 5%; divide it into packages and sterilize it by γ-ray irradiation (γ-ray dose is 25 kGy) to obtain a highly efficient water-locking and repairing facial mask. Example 5

[0052] In this example, the fermented liquid of Astragalus membranaceus extract is prepared according to the preparation method of Preparation Example 2, and the fermented and lysed liquid of Lactobacillus plantarum is prepared according to the preparation method of Preparation Example 5. This embodiment provides an efficient water-locking and repairing facial mask, which comprises the following raw materials by mass fraction: 0.5% Astragalus membranaceus extract fermentation broth (0.5 g), 0.2% ceramide NP (0.2 g), 0.05% sodium hyaluronate composition (0.05 g), 0.1% polyquaternium-51 (0.1 g), 1% Lactobacillus plantarum fermentation lysate (1 g), 96.15% deionized water (96.15 g), 2% ethanol (2 g).

[0053] This embodiment also provides a preparation method of the efficient water-locking and repairing facial mask, which comprises the following steps: A1. Premix the Astragalus membranaceus extract fermentation broth, sodium hyaluronate composition, polyquaternium-51, Lactobacillus plantarum fermentation lysate and deionized water to obtain a premixed solution; A2. Introduce the premixed solution into a Y-shaped microfluidic chip from two branch channels 1 (with a diameter of 200 μm) and flow out from the main channel 2 (with a diameter of 500 μm) to form a uniform colloid; The included angle between the two branch channels 1 is 10°, control the system temperature at 10°C, the pressure at 30 MPa, the flow rate of the main channel 2 at 0.5 mL / min, and the flow rate of the branch channel 1 at 0.25 mL / min; A3. Dissolve ceramide NP in ethanol, add the colloid obtained in step A2, ultrasonically treat it at 30 kHz and 40°C for 5 minutes, and then permeate it at a negative pressure of -0.05 MPa for 10 minutes to obtain a colloid loaded with ceramide NP; A4. Inject the colloid loaded with ceramide NP into a silica gel microporous mold (with a pore diameter of 100 μm), vacuum dry it at -20°C (pressure ≤ 10 Pa) for 1 hour until the moisture content ≤ 6%, package it and sterilize it by γ-ray irradiation (γ-ray dose is 20 kGy) to obtain the efficient water-locking and repairing facial mask. Example VI

[0054] In this embodiment, the Astragalus membranaceus extract fermentation broth is prepared according to the preparation method of Preparation Example 3, and the Lactobacillus plantarum fermentation lysate is prepared according to the preparation method of Preparation Example 6; This embodiment provides an efficient water-locking and repairing facial mask, which comprises the following raw materials by mass fraction: 1% Astragalus membranaceus extract fermentation broth (1 g), 0.3% ceramide NP (0.3 g), 0.3% sodium hyaluronate composition (0.3 g), 0.15% polyquaternium-51 (0.15 g), 3% Lactobacillus plantarum fermentation lysate (3 g), 92.25% deionized water (92.25 g), 3% ethanol (3 g).

[0055] This embodiment also provides a preparation method of the efficient water-locking and repairing facial mask, which comprises the following steps: A1. Premix the Astragalus membranaceus extract fermentation broth, sodium hyaluronate composition, polyquaternium-51, Lactobacillus plantarum fermentation lysate and deionized water to obtain a premixed solution; A2. Introduce the premixed solution into a Y-shaped microfluidic chip through two branch channels 1 (with a diameter of 200 μm) and flow out through the main channel 2 (with a diameter of 500 μm) to form a uniform colloid; The included angle between the two branch channels 1 is 150°. Control the system temperature at 8 °C, the pressure at 60 MPa, the flow rate of the main channel 2 at 2 mL / min, and the flow rate of the branch channel 1 at 1 mL / min; A3. Dissolve ceramide NP in ethanol, add the colloid obtained in step A2, and after ultrasonic treatment at 50 kHz and 50 °C for 15 minutes, infiltrate at a negative pressure of -1 MPa for 20 minutes to obtain a colloid loaded with ceramide NP; A4. Inject the colloid loaded with ceramide NP into a silica gel microporous mold (with a pore diameter of 100 μm), and vacuum dry at -20 °C (pressure ≤ 10 Pa) for 3 hours until the moisture content ≤ 5%; Subpackage and sterilize by γ-ray irradiation (γ-ray dose is 30 kGy) to obtain a highly efficient water-locking and repair facial mask.

[0056] Comparative Example 1 In this comparative example, the Astragalus membranaceus extract fermentation broth was prepared according to the preparation method of Preparation Example 1, and the Lactobacillus plantarum fermentation lysate was prepared according to the preparation method of Preparation Example 4; The raw material ratio of the facial mask in this comparative example is the same as that in Example 1; The preparation method of the facial mask in this comparative example includes the following steps: Add the Astragalus membranaceus extract fermentation broth, sodium hyaluronate composition, polyquaternium-51, Lactobacillus plantarum fermentation lysate to 80 °C deionized water, and magnetically stir at 800 rpm for 1 h until completely dissolved to obtain a colloid; Dissolve ceramide NP in ethanol, add the above colloid, stir at 10000 rpm in a homogenizer for 5 min, then naturally cool to 40 °C, and inject into a silica gel microporous mold (with a pore diameter of 100 μm) for room temperature curing.

[0057] Comparative Example 2 In this comparative example, the Astragalus membranaceus extract fermentation broth was prepared according to the preparation method of Preparation Example 1, and the Lactobacillus plantarum fermentation lysate was prepared according to the preparation method of Preparation Example 4; The raw material ratio of the facial mask in this comparative example is the same as that in Example 1, except that an equal amount of Astragalus membranaceus extract fermentation broth is used to replace ceramide NP in this comparative example; The facial mask in this comparative example was prepared according to the steps of Example 1, except that step A3 was omitted in this comparative example, and the colloid formed in step A2 was directly injected into the silica gel microporous mold.

[0058] Comparative Example 3 In this comparative example, the Astragalus membranaceus extract fermentation broth was prepared according to the preparation method of Preparation Example 1, and the Lactobacillus plantarum fermentation lysate was prepared according to the preparation method of Preparation Example 4; The raw material ratio of the facial mask in this comparative example was the same as that in Example 1, except that in this comparative example, the Astragalus membranaceus extract fermentation broth was 0.5% and ceramide NP was 0.5%; The facial mask in this comparative example was prepared according to the steps of Example 1.

[0059] Comparative Example 4 In this comparative example, the Astragalus membranaceus extract fermentation broth was prepared according to the preparation method of Preparation Example 1, and the Lactobacillus plantarum fermentation lysate was prepared according to the preparation method of Preparation Example 4; The raw material ratio of the facial mask in this comparative example was the same as that in Example 1; The preparation method of the facial mask in this comparative example: Mix the Astragalus membranaceus extract fermentation broth, ceramide NP, sodium hyaluronate composition, polyquaternium-51, Lactobacillus plantarum fermentation lysate, ethanol, and deionized water, stir at 500 rpm at 25 °C for 2 hours, and then directly inject into a silica gel microporous mold (pore size 100 μm) and cure at room temperature.

[0060] Comparative Example 5 In this comparative example, the Lactobacillus plantarum fermentation lysate was prepared according to the preparation method of Preparation Example 4; The raw material ratio of the facial mask in this comparative example was the same as that in Example 1, except that an equal amount of Astragalus membranaceus extract without Aspergillus niger fermentation was used instead of the Astragalus membranaceus extract fermentation broth; The facial mask in this comparative example was prepared according to the steps of Example 1, except that in step A1, the Astragalus membranaceus extract was used instead of the Astragalus membranaceus extract fermentation broth.

[0061] Comparative Example 6 In this comparative example, the Lactobacillus plantarum fermentation lysate was prepared according to the preparation method of Preparation Example 4; The raw material ratio of the facial mask in this comparative example was the same as that in Example 1, except that an equal amount of ceramide NP was used instead of the Astragalus membranaceus extract fermentation broth in this comparative example; The facial mask in this comparative example was prepared according to the steps of Example 1, except that there was no Astragalus membranaceus extract fermentation broth in step A1 of this comparative example.

[0062] Comparative Example 7 In the comparative example, the Astragalus membranaceus extract fermentation broth was prepared according to the preparation method of Preparation Example 1; The raw material ratio of the facial mask in this comparative example is the same as that in Example 1, except that an equal amount of exogenous propionic acid / butyric acid mixture is used to replace the plant lactic acid bacteria fermentation lysate. In the exogenous propionic acid / butyric acid mixture, the mass content of propionic acid is 1% and the mass content of butyric acid is 0.8%, and the balance is deionized water.

[0063] Performance tests were carried out on the facial masks of Examples 1 - 6 and Comparative Examples 1 - 7, and the data in Tables 1 and 2 were obtained respectively.

[0064] I. Determination of transepidermal water loss rate (TEWL) Test instrument: Tewameter® TM 300 (produced by CK Electronic GmbH, Germany, calibration certificate number CE - 2023 - 0456) Test standard: According to T / ZHCA 003 - 2018 "Test Method for Cosmetics Affecting Transepidermal Water Loss" Experimental conditions: Test environment: Thermostatic and humidified chamber (25.0 ± 0.5 °C, relative humidity 50 ± 5%RH). The subjects sat still for 30 minutes to adapt, and after cleaning the test area (flexor side of the forearm), they were left standing for 15 minutes.

[0065] Test population: 130 healthy female volunteers (aged 18 - 45 years old, Fitzpatrick skin type III - IV), divided into 13 groups for rotation testing, with 10 people in each group; Construction of damage model: The tape stripping method (3M™ Transpore™ 1527 - 1, continuously stripped 15 times) was used to simulate barrier damage, and the initial TEWL value was recorded as the baseline ( )

[0066] Operation process: (1) Moisten the facial mask with a small amount of water and apply it to the test area (dose 5 mg / cm²), with a coverage area of 4 × 4 cm² and an action time of 20 minutes; (2) Remove and gently wipe with deionized water, and let the residue dry naturally; (3) Continuously measure the TEWL value at 0.5h, 1h, 2h, 4h, 6h, 8h, and 24h after treatment, and record the time when it returns to 90% of the baseline ( )

[0067] Data recording: Each group of tests was repeated 3 times, and the arithmetic mean was taken, with the standard deviation ≤ 0.8 g / m² / h.

[0068] II. Detection of stratum corneum water content Testing instrument: Corneometer® CM 825 (CK Electronic GmbH, Germany, probe model MPA-580) Testing standard: Refer to Appendix B of QB / T 4256-2011 "Evaluation Guidelines for Moisturizing Efficacy of Cosmetics" Experimental procedures: Subject population: 130 healthy female volunteers (aged 18 - 45 years old, testing area is the cheek, without inflammation or damage) 24 hours before the test, divided into 13 groups for rotation testing, 10 people in each group; Pre-treatment of the subjects' faces Gently clean the face with a neutral detergent (pH 5.5 - 7.0), avoiding friction and damage to the stratum corneum; rinse with pure water at 25°C for 10 seconds, and dry the moisture with a sterile gauze; the subjects sit quietly for 30 minutes in a constant temperature and humidity environment (temperature 22 ± 2°C, humidity 50 ± 5%), exposing the test area to stabilize the skin condition.

[0069] After the subjects operate according to the pre-treatment requirements, take the test mask, moisten it with a small amount of water and apply it evenly on the face, avoiding the eye and lip areas; apply for 15 ± 2 minutes; remove the mask after use, and gently pat the remaining essence until absorbed; 8 hours after the mask is used, use the Corneometer® probe perpendicular to the skin surface, apply a constant pressure (3.5 N) for 5 seconds, read the capacitance value and convert it to the water content percentage (instrument built-in algorithm, range 0 - 120 a.u.); Collect 10 points for each group of data, and take the mean value after excluding the highest and lowest values.

[0070] III. Analysis of the order degree of stratum corneum lipids Testing equipment: LabRAM HR Evolution laser confocal Raman spectrometer (HORIBA Scientific, France, excitation wavelength 532 nm, grating 1800 lines / mm) Subject population: 130 healthy female volunteers (aged 18 - 45 years old, Fitzpatrick skin type III - IV), divided into 13 groups for rotation testing, 10 people in each group; Pre-treatment requirements: Gently clean the face with a neutral detergent (pH 5.5 - 7.0), avoiding friction and damage to the stratum corneum; rinse with pure water at 25°C for 10 seconds, and dry the moisture with a sterile gauze; the subjects sit quietly for 30 minutes in a constant temperature and humidity environment (temperature 22 ± 2°C, humidity 50 ± 5%), exposing the test area to stabilize the skin condition.

[0071] After the subjects meet the pre-treatment requirements, they take the test facial mask, moisten it with a small amount of water and apply it evenly on the face, avoiding the eye and lip areas; apply for 15 ± 2 minutes; after use, remove the facial mask and gently pat the remaining essence until absorbed, without performing secondary cleansing; Test method: (1) Sampling is carried out by the method of Tape Stripping. Use 3M™ D-Squame® standard adhesive tape and paste it on the skin surface of the subject to obtain a sample containing stratum corneum lipids, and place the sample containing stratum corneum lipids on a quartz slide; Spectral detection: Use a confocal Raman spectrometer to collect the characteristic spectrum of stratum corneum lipids, and the focusing depth ≤ 20μm; (3) Use Gaussian fitting to analyze the characteristic peak of the lipid chain order at 2920 cm -1 , and calculate the full width at half maximum (FWHM). The smaller the value, the more orderly the lipid arrangement.

[0072] Data verification: Test 5 different regions for each group of samples, take the mean value after removing outliers, and the instrument resolution ≤ 1 cm -1 .

[0073] IV. Clinical stinging rate (%) Reference standard: ISO 10993-10:2021 "Biological evaluation of medical devices - Part 10: Tests for irritation and skin sensitization" Test method (1) Subject screening Select 140 healthy female volunteers (aged 18 - 45 years old, Fitzpatrick skin type III - IV), excluding those with a history of skin sensitivity, allergic constitution, and recent use of immunosuppressants. Sign an informed consent form before the test; (2) Test plan Adopt a single-blind controlled trial. Moisten the facial mask with a small amount of water and apply it on the inner forearm of the subject (dose 5 mg / cm², area 2×2 cm²), once a day, 15 minutes each time, for 7 consecutive days. The control group uses physiological saline for wet compress; (3) Assessment of stinging reaction Assessment time points: 0.5h, 1h, 24h after application; Score according to Table 1 Table 1 Scoring criteria

[0074] Calculation of stinging rate: The proportion of subjects with a score ≥ 1.

[0075] Table 2 Barrier repair performance

[0076] Comparing Comparative Example 2, Comparative Example 6 with Example 1, it can be seen that when Astragalus membranaceus extract fermentation broth or ceramide NP exists alone, in terms of barrier repair, moisturizing, and lipid layer order, it is inferior to Example 1, indicating that the Astragalus membranaceus extract fermentation broth and ceramide NP in the present invention play a synergistic role. The hydrophobic chain of ceramide NP forms a biomimetic arrangement with the polar groups of β-1,4-glucan, and the lipid order is close to that of healthy skin ( ). The absence of ceramide NP leads to disorder of the lipid layer, indirectly proving the synergistic effect of ceramide and β-1,4-glucan. Ceramide NP fills the lipid gap, and β-1,4-glucan strengthens the network skeleton, and neither of them can be absent. The β-1,4-glucan in the Astragalus membranaceus extract fermentation broth and ceramide NP form a "lipid-polysaccharide interpenetrating network" through electrostatic self-assembly, synergistically simulating the natural skin barrier structure, significantly shortening the time required for TEWL recovery, improving the lipid order of the stratum corneum, enhancing the water-locking ability, and having low irritation. Comparing Comparative Example 1, Comparative Example 4 with Example 1, it is found that high temperature (80 °C) causes partial oxidation of ceramide NP and degradation of β-1,4-glucan, while conventional stirring reduces the uniformity of the raw material distribution in the system, resulting in the components not being able to fully play their roles, indicating that the low-temperature microfluidic process and vacuum freeze-drying in the present invention can greatly retain the active ingredients, improve the uniformity of the system, and maximize the synergistic effect.

[0077] Comparing Comparative Example 3 with Example 1, it can be seen that when the mass ratio of Astragalus membranaceus extract fermentation broth to ceramide NP is 1:1, the excessive amount of ceramide NP leads to excessive lipid accumulation and forms a disordered structure.

[0078] Comparing Comparative Example 5 with Example 1, it can be seen that the sensitizing saponins in the unfermented Astragalus membranaceus extract increase the clinical stinging rate (%) to 5.2%, and the macromolecular impurities in it interfere with the formation of the "lipid-polysaccharide interpenetrating network", resulting in the active ingredients not being able to fully contact the skin and play their roles.

[0079] Comparing Comparative Example 7 with Example 1, it can be seen that the clinical stinging rate of the externally added butyric acid / propionic acid mixture is relatively high, at 4.5%, and long-term use may lead to microecological imbalance. The exogenous organic acid also interferes with the hydration ability of sodium hyaluronate, and the water content is significantly lower than that of Example 1, indicating that the plant lactobacillus fermentation lysate of the present invention has higher safety. From the data in Table 1, it can be seen that the plant lactobacillus fermentation lysate also plays a role in repairing the barrier and locking water.

[0080] In summary, in the present invention, the time for TEWL to recover to 90% of the baseline is less than or equal to 10.5 ± 1.1 h, the water content of the stratum corneum (%) at 8 hours is greater than or equal to 39.4 ± 1.2, and the lipid order (full width at half maximum, cm -1Less than or equal to 21.5 ± 0.6, and the clinical stinging rate is below 3.8%. When the mass ratio of ceramide NP to the fermented liquid of Astragalus membranaceus extract is 1:3, the synergistic effect is the greatest. The time for TEWL to recover to 90% of the baseline is only 6.2 ± 0.5 h, the water content of the stratum corneum (%) at 8 hours is 58.3 ± 2.1, and the lipid order parameter (full width at half maximum, cm -1 is 14.8 ± 0.3, close to that of healthy skin (12 - 14 cm -1 ), and the clinical stinging rate (%) is 1.2.

[0081] V. Molecular weight retention rate of β-1,4-glucan in the fermented liquid of Astragalus membranaceus extract (1) High-performance liquid chromatography was used in the experiment. Chromatographic conditions: TSKgel GMPWXL chromatographic column (7.8 × 300 mm), with 0.1 M phosphate buffer (pH 6.8) as the mobile phase (0.5 mL / min, 35 °C), flow rate: 1.0 mL / min, column temperature: 30 °C, injection volume 10 μL, coupled with MALLS (658 nm, 18 angles) and RID detector; (2) Sample pretreatment: Mask sample: The mask was wetted with deionized water (mass ratio of mask to deionized water 1:10), centrifuged at 10,000 rpm for 10 min, and the supernatant was taken and filtered through a 0.22 μm filter membrane; Control sample: The fermented liquid of Astragalus membranaceus extract corresponding to the same batch of each mask was filtered through a 0.22 μm filter membrane; (3) Inject the mask sample and the control sample respectively, and record the molecular weight distribution curve; (4) Calculate the proportion of the peak area in the target molecular weight range (5 - 50 kDa, β-1,4-glucan); Calculation of retention rate: Molecular weight retention rate (%) = (peak area of the target range of the mask sample / peak area of the target range of the control sample) × 100%; VI. Determination of ceramide NP encapsulation rate Detection instrument: Agilent 1260 Infinity II HPLC system (Agilent Technologies, USA, C18 chromatographic column, 4.6 × 250 mm, 5 μm) Chromatographic conditions: Mobile phase: acetonitrile (containing 0.1% trifluoroacetic acid) - water = 70:30 (v / v) Flow rate: 1.0 mL / min, column temperature: 30 °C, injection volume 10 μL, detection wavelength: 205 nm; Sample treatment: Total content sample: (1) Cut 1 g of the facial mask into pieces and extract it in 5 mL of methanol. Sonicate for 30 minutes, add 10 mL of a methanol-chloroform mixture (2:1, v / v), and vortex for 5 minutes. (2) Centrifuge at 10000 rpm for 15 minutes. Take the supernatant and filter it through a 0.22 μm organic filter membrane for later use. Free content sample: Take 1 g of the facial mask and extract it in 5 mL of sterile water. Directly add it to an ultrafiltration centrifugal tube (with a cut-off molecular weight of 3 kDa) and centrifuge at 4000 rpm for 20 minutes. Collect the lower filtrate (containing free ceramide NP) and filter it through a 0.22 μm aqueous filter membrane. Standard curve plotting Weigh accurately the ceramide NP standard product, dissolve it with acetonitrile-water (volume ratio 70:30), and prepare gradient concentration solutions of 0.1 - 100 μg / mL. Inject for analysis, and perform linear regression with the peak area (Y) against the concentration (X) (R²≥0.999). Sample detection Inject the total content sample (filtrate after destroying the facial mask) and the free content sample (ultrafiltration filtrate) respectively. Calculate the concentration based on the peak area, and calculate the encapsulation efficiency according to the following formula: Encapsulation efficiency (%) = [(total content - free content) / total content] × 100%.

[0082] VII. Biodegradability assessment Test standard: OECD 301B "Ready Biodegradability: Modified Sturm Test" Experimental device: Closed bottle method, constant temperature shaker (25±1°C, protected from light) Operation process: (1) Prepare the test solution: Take 1.0 g of the facial mask (dry weight), make up the volume to 100 mL with water. Take an appropriate amount of the supernatant and dilute it with the OECD301B standard mineral salt solution to adjust the organic carbon concentration of the test solution to 80 mg / L; adjust the initial DOC (dissolved organic carbon) concentration to 25 mg / L. (2) Inoculate with activated sludge (collected from a municipal sewage treatment plant, MLSS = 3 g / L), set up a blank control and a reference substance (sodium benzoate). Blank control: containing only activated sludge and culture medium. (3) Take samples on days 0, 7, 14, 21, and 28, and measure the DOC removal rate through a TOC analyzer (Shimadzu TOC-L CPH).

[0083] Judgment criterion: If the DOC removal rate ≥ 60% within 28 days, it is considered "readily biodegradable".

[0084] VIII. Viscosity stability (1)Test using a Brookfield DV2T rotational viscometer (LV-3 rotor, 60 rpm) in a constant temperature water bath at 25.0 ± 0.1 °C; (2)Take 10.0 ± 0.1 g of the freeze-dried mask, let it stand at 25 °C for 24 hours, and measure the apparent viscosity at time points of 0, 7, 15, and 30 days; (3)The viscosity change rate is calculated according to the formula Initial viscosity (η0): The viscosity at day 0, the average value is determined by three parallel experiments (RSD ≤ 2%).

[0085] Final viscosity (η t ): The viscosity at day 30, the average value is determined by three parallel experiments (RSD ≤ 2%).

[0086] Viscosity change rate formula: Viscosity change rate = |( ) / η0| × 100% Data validity verification: The viscosity change rate of the blank control (pure water) within 30 days ≤ 0.5%, and the test system meets the requirements.

[0087] Table 3 Process stability and active ingredient retention

[0088] Comparing Comparative Example 1 with Example 1 in Table 3 shows that the low-temperature microfluidic process of the present invention combined with the vacuum freeze-drying process effectively inhibits the thermal degradation of β-1,4-glucan and is significantly superior to Comparative Example 1 (68.4%) in terms of improving the retention rate of β-1,4-glucan in the highly efficient water-locking and repairing mask.

[0089] Moreover, the encapsulation rate of ceramide NP and the viscosity change rate of Example 1 of the present invention are also superior to those of Comparative Example 4. The encapsulation rate of ceramide NP is increased by 41.7% compared with Comparative Example 4 (65.7%), indicating that the laminar shear of the microfluidic process in the present invention makes the raw materials form a uniform nano-scale colloid, providing a good carrier for loading ceramide NP. At the same time, through the synergistic action of ultrasound-negative pressure, transient channels are formed in the colloid, promoting the penetration of ceramide NP molecules through the pores of the colloid to construct a repair barrier with β-1,4-glucan, weakening the solvation layer of ceramide NP and improving the encapsulation rate.

[0090] In addition, compared with the high-temperature emulsification in Comparative Example 1 and the normal-pressure stirring in Comparative Example 4, the viscosity change rate of the uniform nanofiber network formed by the microfluidic process of the present invention is lower and the stability is better. In terms of biodegradation, the low encapsulation rate in Comparative Example 1 and Comparative Example 4 results in the exposure of ceramide NP on the surface, directly contacting microbial lipase and accelerating degradation. Moreover, the mask structure is disordered and porous, and microorganisms can quickly invade and secrete extracellular enzymes, leading to rapid degradation.

[0091] Comparison between Example 3 and Example 2 shows that the ratio of the fermented liquid of Astragalus membranaceus extract to ceramide NP in Comparative Example 3 is unbalanced, resulting in the separation of ceramide NP-β-1,4-glucan, which destroys the stability of the structure. Comparative Example 5 is compared with Example 1. The unfermented Astragalus membranaceus extract in Comparative Example 5 contains endogenous enzymes, which cause the β-1,4-glucan molecular chain to break, thereby affecting the encapsulation rate of ceramide NP and the construction of the "lipid-polysaccharide interpenetrating network", and reducing the skin barrier repair and water lock ability.

[0092] In summary, in the high-efficiency water-locking and repairing mask prepared by the present invention, the molecular weight retention rate of the fermentation broth of Astragalus membranaceus extract is greater than or equal to 89.7±1.8%, the ceramide NP encapsulation rate (%) is greater than or equal to 78.4±2.3, the viscosity change rate is less than or equal to 12%, and the biodegradation rate (28 days, %) is between 70.1±2.3 and 85.6±3.1, indicating that the survival rate of the effective ingredients in the present invention is high, and a stable nanonetwork is formed by microfluidic technology to improve the ceramide NP encapsulation rate and maintain the sustained release of the active ingredients; ceramide NP is encapsulated in the core of the nanofiber network, and microorganisms need to decompose the outer layer of β-1,4-glucan before they can contact the ceramide NP lipids, which delays the overall degradation process, which can not only ensure effective degradation within 28 days (in line with the ISO 14855 "compostable material" standard), but also maintain shelf stability for at least 12 months (viscosity change ≤12%).

[0093] It should be understood that the purpose of these embodiments is only to illustrate the present invention and is not intended to limit the protection scope of the present invention. In addition, it should also be understood that after reading the technical content of the present invention, those skilled in the art can make various changes, modifications and / or variations to the present invention, and all of these equivalent forms also fall within the protection scope defined by the claims attached to this application.

Claims

1. An efficient water-locking repair facial mask, characterized in that, Components containing the following mass percentages: Astragalus membranaceus extract fermentation broth 0.5 - 2.0%, ceramide NP 0.1 - 0.5%, sodium hyaluronate composition 0.05 - 0.3%, polyquaternium - 51 0.1 - 0.3%, Lactobacillus plantarum fermentation lysate 1 - 3%, ethanol 1 - 5%, and the balance being deionized water; The Lactobacillus plantarum fermentation lysate includes propionic acid and butyric acid; The preparation method of the Astragalus membranaceus extract fermentation broth includes the following steps: (1) Mix 5 - 15 g of Astragalus membranaceus extract with the Aspergillus niger strain with the preservation number of CICC 2487 ( Aspergillus niger ) to obtain a mixture, and the inoculation amount of the Aspergillus niger strain is 5 - 10% of the mass of the Astragalus membranaceus extract; (2) Prepare a solid fermentation medium, which includes the following components by mass fraction: wheat bran 2 - 5%, potassium dihydrogen phosphate 0.1 - 0.3%, magnesium sulfate 0.02 - 0.07%, and the balance being water; (3) Add the mixture from step (1) to the solid fermentation medium in step (2) and place it in a fermentation container. Ferment at 20 - 40 °C and pH = 5 - 7 for 50 - 70 hours to obtain a fermentation product. Stir at 20 - 40 rpm for 1 - 10 minutes every 5 - 7 hours during fermentation; (4) Subject the fermentation product to ultrasonic treatment, centrifugation, and ultrafiltration to obtain the Astragalus membranaceus extract fermentation broth; The mass ratio of the mixture in step (1) to the solid fermentation medium in step (2) is 1:1.

5.

2. The highly efficient water-locking repair facial mask according to claim 1, wherein In step (4), the fermentation product is ultrasonically extracted at 30 - 50 kHz and 40 - 60 °C for 15 - 20 min; In step (4), ultrafiltration treatment is carried out using an ultrafiltration tube, and the cut - off molecular weight of the ultrafiltration tube is 5 - 50 kDa.

3. The highly efficient water-locking repair facial mask according to claim 1, characterized in that, The mass ratio of the ceramide NP to the Astragalus membranaceus extract fermentation broth is (1:2) - (1:5).

4. The highly efficient water-locking repair facial mask according to claim 1, characterized in that The sodium hyaluronate composition is composed of sodium hyaluronate with molecular weights of 1 kDa, 100 kDa, and 1 MDa compounded according to a mass ratio of 1:2:

1.

5. The high-efficiency water-locking repair facial mask according to claim 1, characterized in that, The preparation method of the Lactobacillus plantarum fermentation lysate includes the following steps: S1. Add the extract of Astragalus membranaceus and Lactobacillus plantarum with the preservation number of CICC 25125 ( Lactobacillus plantarum ) to the MRS liquid medium, and ferment at 30 - 40 °C for 40 - 60 hours to obtain the fermentation product of Lactobacillus plantarum; S2. Subject the Lactobacillus plantarum fermentation product to centrifugation and filtration to obtain the Lactobacillus plantarum fermentation lysate; In the Lactobacillus plantarum fermentation lysate, the mass content of propionic acid is 0.5 - 1.5%, and the mass content of butyric acid is 0.3 - 1.2%; The mass - to - volume ratio of the Astragalus membranaceus extract to the MRS liquid medium is (5 - 15%):1; The mass ratio of the Lactobacillus plantarum to the MRS liquid medium is (5 - 10%):1; In step S2, sterile filtration is carried out using a 0.22 - um microporous membrane.

6. A method for preparing an efficient water-locking repair facial mask according to any one of claims 1-5, characterized in that, Include the following steps: A1. Premix the Astragalus membranaceus extract fermentation broth, sodium hyaluronate composition, polyquaternium - 51, Lactobacillus plantarum fermentation lysate, and deionized water to obtain a premixed solution; A2. Introduce the premixed solution into a Y - type microfluidic chip to form a uniform colloid; A3. Dissolve the ceramide NP in ethanol, add it to the colloid in step A2, carry out ultrasonic treatment at 40 - 50 °C and 30 - 50 kHz for 5 - 15 minutes, and then carry out permeation at a negative pressure of - 0.05 to - 1 MPa for 10 - 20 minutes to obtain a colloid loaded with ceramide NP; A4. Inject the colloid loaded with ceramide NP into a silica microporous mold, freeze-dry it under vacuum at -20°C for 1 - 3 hours until the moisture content ≤ 10%, divide it into portions and sterilize it by gamma-ray irradiation to obtain a highly efficient water-locking repair facial mask; The mass ratio of the ceramide NP to ethanol is 1:10; The pore diameter of the silica microporous mold is 100 μm, and the dose of the gamma-ray is 20 - 30 kGy; The system temperature of the Y-shaped microfluidic chip is 5 - 10°C, and the pressure is 30 - 60 MPa.

7. The preparation method of the high-efficiency water-locking repair facial mask according to claim 6, characterized in that, The Y-shaped microfluidic chip includes a main channel and two branch channels. The main channel and the two branch channels are interconnected, and the included angle between the two branch channels is 10 - 150°; The diameter of the main channel is 500 μm, and the flow rate of the main channel is 0.5 - 2 mL / min; The diameter of the branch channel is 200 μm, and the flow rate of the branch channel is 0.25 - 1 mL / min.

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