Preparation method and application of novel stomach and intestinal tract passing sustained-release tannic acid

Abstract

The invention belongs to the technical field of materials, discloses a preparation method and application of novel stomach and intestinal tract passing sustained-release tannic acid, and particularly discloses a stomach and intestinal tract passing enteric coating material which comprises a surfactant, sugar alcohol, hydrogenated grease, polyacrylic resin and a thickening agent. The stomach-passing enteric coating material does not use expensive commercially available enteric coating materials, but adopts common surfactants, sugar alcohol substances and other substances, so that the grease and the water can be better fused, and the grease can be better coated outside in the coating process. The stomach-passing enteric coating material can ensure that tannic acid is not damaged by gastric acid, reduces the influence on animal digestive enzymes, and can be slowly released in intestinal tracts to be absorbed and utilized.

Description

Technical Field

[0001] The present invention belongs to the technical field of materials, and particularly relates to a preparation method and application of a novel gastric- and intestinal-passable sustained-release tannic acid. Background Art

[0002] Tannic acid is a natural polyphenolic compound, soluble in water, with a relative molecular mass of 500 - 3000, and is mainly present in plants such as Chinese gallnut, wattle, and chestnut wood. Research shows that adding tannic acid to the diet has a significant positive effect on the growth and disease resistance of livestock and poultry, such as antibacterial, anti-inflammatory, anti-diarrheal, promoting intestinal health, and effectively eliminating internal parasites.

[0003] There have been many studies on tannic acid in piglets. Most studies show that adding 0.7 - 1.5 kg / t of tannic acid to the diet of weaned piglets can enhance their digestive ability, reduce the diarrhea rate, improve production performance, and reduce the use of antibiotics, especially zinc oxide. However, the production cost of adding 0.7 - 1.5 kg / t of hydrolyzed tannic acid is much higher than that of adding 2.0 kg / t of zinc oxide. Therefore, according to the addition amount of the research data, it is difficult for breeding companies or farmers to bear the cost of tannic acid. In addition, some studies have found that directly adding the original powder of tannic acid without processing and coating treatment to the diet of piglets will significantly reduce the feed intake of piglets and thus affect production performance. The reason may be that tannic acid, as an antinutritional factor in feed raw materials, will combine with proteins in the feed to form insoluble complexes, thereby affecting digestion. Moreover, after tannic acid enters the digestive tract of animals, it will also combine with proteins in the gastrointestinal mucosa to form insoluble complexes on the surface of the intestinal mucosa, damaging the gastrointestinal morphology and reducing the animal's ability to digest and absorb nutrients. Therefore, it is necessary to subject tannic acid to gastric-pass treatment to enable tannic acid to be concentrated and released at the end of the small intestine and the large intestine, reducing the usage amount of tannic acid and its adverse effects on animals.

[0004] Regarding the research data of tannic acid, the high addition amount has too high a cost and is difficult to replace antibiotics for application in the breeding industry. However, by coating tannic acid, the loss of tannic acid can be reduced before absorption, which can reduce the tannin content of tannic acid products and effectively reduce the usage cost. The prior art discloses coating tannic acid with enteric-coated tablets. Due to material reasons, it can pass through the stomach, but the materials used are complex and expensive, the intestinal sustained-release effect is poor, and the production process is complex, so it has not been effectively applied in the market. Summary of the Invention

[0005] The purpose of the first aspect of the present invention is to provide a gastric- and enteric-coated material.

[0006] The purpose of the second aspect of the present invention is to provide a coated tannic acid.

[0007] The object of the third aspect of the present invention is to provide a preparation method of tannic acid-coated of the second aspect of the present invention.

[0008] The object of the fourth aspect of the present invention is to provide an application of the tannic acid-coated of the second aspect of the present invention in the preparation of feed or feed additives.

[0009] The object of the fifth aspect of the present invention is to provide a feed or feed additive.

[0010] In order to achieve the above object, the technical solutions adopted by the present invention are as follows:

[0011] The first aspect of the present invention provides a stomach-passage and enteric-coated material, including a surfactant, a sugar alcohol, a hydrogenated oil, a polyacrylic resin and a thickener.

[0012] In some embodiments of the present invention, the surfactant includes at least one of ammonium phospholipid, mono / diglycerol fatty acid ester, glycerol succinate, polyglycerol ricinoleate, polyglycerol fatty acid ester, polyoxyethylene xylitan monostearate, Tween (Tween 20, Tween 40, Tween 60, Tween 80), xylitan monostearate, citric acid glycerol fatty acid ester, hydrogenated rosin glycerol ester, lactic acid glycerol fatty acid ester, Span (Span 20, Span 40, Span 60, Span 65, Span 80), diacetyl tartaric acid mono / diglyceride, sulfosuccinate salt, fatty acid sulfonate and sodium stearoyl lactate.

[0013] In some embodiments of the present invention, the thickener includes at least one of octenyl succinic anhydride distarch phosphate, propylene glycol ester, antacid carboxymethyl cellulose sodium, hydroxypropyl starch, pectin, xanthan gum, alginate, carrageenan, agar, modified starch, guar gum, locust bean gum, konjac gum, pectin, low-fat pectin, arabic gum and sodium polyacrylate.

[0014] In some embodiments of the present invention, the sugar alcohol includes at least one of propylene glycol alginate, mannitol, pectin, soluble soybean polysaccharide, maltitol, lactitol and sorbitol.

[0015] In some embodiments of the present invention, the hydrogenated oil includes at least one of hydrogenated palm oil, hydrogenated rapeseed oil, hydrogenated soybean oil, hydrogenated coconut oil, hydrogenated castor oil, palm stearin, hydrogenated peanut oil and hydrogenated corn oil.

[0016] In some embodiments of the present invention, by mass, the stomach-passage and enteric-coated material includes 100-300 parts of surfactant, 80-150 parts of sugar alcohol, 1000-1500 parts of hydrogenated oil, 80-130 parts of polyacrylic resin and 10-30 parts of thickener.

[0017] In some embodiments of the present invention, by mass parts, the gastric-passage and enteric-coated material comprises 100-250 parts of surfactant, 100-150 parts of sugar alcohol, 1200-1500 parts of hydrogenated oil, 100-130 parts of polyacrylic resin, and 15-30 parts of thickener.

[0018] In some embodiments of the present invention, by mass parts, the gastric-passage and enteric-coated material comprises 200-250 parts of surfactant, 100-120 parts of sugar alcohol, 1200-1300 parts of hydrogenated oil, 100-110 parts of polyacrylic resin, and 15-25 parts of thickener.

[0019] In some embodiments of the present invention, the gastric-passage and enteric-coated material further comprises water.

[0020] In some embodiments of the present invention, the mass ratio of water to the total mass of other components in the gastric-passage and enteric-coated material is 1:1-2.

[0021] In some embodiments of the present invention, the preparation method of the gastric-passage and enteric-coated material comprises the following steps:

[0022] Mix the surfactant, sugar alcohol, polyacrylate, thickener with water, stir and dissolve for 20-60 min until complete dissolution to obtain mixed solution 1;

[0023] Mix the melted hydrogenated palm oil with mixed solution 1 and emulsify to obtain the gastric-passage and enteric-coated material (kept at 60-70 °C).

[0024] In some embodiments of the present invention, the conditions for emulsification are emulsifying at 5000-11000 r / min for 30-80 min.

[0025] In some embodiments of the present invention, an emulsifying tank is selected for emulsification.

[0026] In the second aspect of the present invention, there is provided a coated tannic acid, comprising the gastric-passage and enteric-coated material of the first aspect of the present invention and tannic acid particles.

[0027] In some embodiments of the present invention, the mass ratio of the gastric-passage and enteric-coated material to tannic acid particles is 1:1-3.

[0028] In some embodiments of the present invention, in the coated tannic acid, the mass percentage of tannic acid in the finished coated tannic acid product is 40%-55%.

[0029] In some embodiments of the present invention, in the coated tannic acid, the mass percentage of tannic acid in the finished coated tannic acid product is 45%-55%.

[0030] In some embodiments of the present invention, in the coated tannic acid, the mass percentage of tannic acid in the finished product of the coated tannic acid is 50%.

[0031] In some embodiments of the present invention, the tannic acid particles include tannic acid and a binder.

[0032] In some embodiments of the present invention, the tannic acid particles include 70% - 80% tannic acid and 20% - 30% binder.

[0033] In some embodiments of the present invention, the binder includes mineral materials, starch, and microcrystalline cellulose.

[0034] In some embodiments of the present invention, the mineral materials include: a mixture composed of one or more of stone powder, talc powder, bentonite, montmorillonite, kaolin, titanium dioxide, light calcium carbonate, calcium stearate, or zinc oxide in any proportion.

[0035] In some embodiments of the present invention, the starch includes: a mixture composed of one or several of corn starch, wheat starch, rice starch, tapioca starch, waxy corn starch, potato starch, millet starch, pregelatinized tapioca starch, hydroxypropyl phosphate starch, acetate - modified starch, cross - linked starch, or sodium octenyl succinate starch in any proportion.

[0036] In some embodiments of the present invention, the preparation method of the tannic acid particles includes mixing tannic acid with a binder and granulating to obtain the tannic acid particles.

[0037] In some embodiments of the present invention, the preparation method of the tannic acid particles includes placing tannic acid and a binder in the hopper of a high - efficiency wet mixing granulator, extruding the material at a rotational speed of 30 - 50 rpm and an aperture of 0.5 - 0.8 mm by a low - temperature extruder, and then rounding the tannic acid particles at a rotational speed of 200 - 300 r / min by a rounding machine with a rolling disc diameter of 600 - 700 mm to form the tannic acid particles.

[0038] This gastric - passing and enteric - coated material can ensure that tannic acid is not destroyed by gastric acid, reduce the impact on animal digestive enzymes, and at the same time can be slowly released and absorbed in the intestine, that is, the effect of gastric - passing and intestinal slow - release can be achieved.

[0039] In the third aspect of the present invention, a preparation method of the coated tannic acid of the second aspect of the present invention is provided, including the following steps: mixing the gastric - passing and enteric - coated material and the tannic acid particles, and performing coating in a coating fluidized bed to obtain the coated tannic acid.

[0040] In some embodiments of the present invention, the inlet air temperature of the fluidized bed coater is controlled at 50 - 60 °C, the outlet air temperature is below 40 °C, the wind speed is 25 - 50 Hz, and the coating time is 1 - 2 hours.

[0041] In some embodiments of the present invention, the preparation method further includes sieving.

[0042] In some embodiments of the present invention, 100% of the coated tannic acid passes through a 20-mesh sieve, and less than 5% passes through an 80-mesh sieve.

[0043] A fourth aspect of the present invention provides the use of the coated tannic acid of the second aspect of the present invention in the preparation of feed or feed additives.

[0044] A fifth aspect of the present invention provides a feed or feed additive comprising the coated tannic acid of the second aspect of the present invention.

[0045] In some embodiments of the present invention, the feed further comprises a basal diet.

[0046] In some embodiments of the present invention, the basal diet comprises corn, soybean meal, whey powder, fish meal, acidifier, amino acids, and a multi-vitamin and multi-mineral premix.

[0047] In some embodiments of the present invention, the amino acids include at least one of lysine, methionine, threonine, tryptophan, and valine.

[0048] In some embodiments of the present invention, the multi-vitamin and multi-mineral premix comprises vitamin A, vitamin B2, vitamin B1, pantothenic acid, vitamin B 12 , vitamin D3, vitamin C, vitamin E, vitamin K, biotin, niacin, copper, magnesium, manganese, zinc, and cobalt.

[0049] In some embodiments of the present invention, the feed contains 0.1 w / w% to 0.5 w / w% of the coated tannic acid.

[0050] The beneficial effects of the present invention are as follows:

[0051] The present invention provides a gastric-passage and enteric-coated material. This gastric-passage and enteric-coated material does not use relatively expensive commercially available enteric coating materials, but rather uses relatively common substances such as surfactants and sugar alcohols. These substances can better fuse oil and water, enabling the oil to be better coated on the outside during the coating process. In the stomach, since there is no lipase, the entire coating layer will not be consumed. Under the action of lipase in the intestine, the hydrophilic groups of the surfactant will be exposed, resulting in rapid release, thereby releasing tannic acid. This allows for the efficient utilization of tannic acid. Adding polypropylene resin and thickener can not only enable the gastric-passage and enteric-coated material to pass through the stomach smoothly. The polyacrylic resin will form a dense film after drying, reducing digestion by gastric acid. At the same time, the thickener will make the entire system more stable.

[0052] The present invention provides a coated tannic acid, which can not only reduce the production cost of tannic acid products, but also enable a higher dose of tannic acid to reach the intestine to exert its effect, significantly improving the production performance of weaned piglets (increasing weight gain, improving feed conversion ratio, and reducing diarrhea rate), and reducing the breeding cost.

[0053] For the coated tannic acid provided by the present invention, in a solution with a pH of 1 - 5.5, the dissolution range of tannic acid is controlled within 0% - 30%, in an aqueous solution with a pH of 2 - 4, the dissolution rate range of tannic acid is 10% - 40%; in an aqueous solution with a pH of 6 - 7.5, the release rate range of tannic acid is 50% - 100%, and in an aqueous solution with a pH of 6.2 - 7.0, the release rate range of tannic acid is 80% - 100%.

[0054] The production raw materials of the coated tannic acid provided by the present invention are few, the production process is simple, and the cost is relatively low. Detailed Embodiments

[0055] The content of the present invention will be further described in detail below through specific embodiments.

[0056] It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention.

[0057] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be described clearly and completely below. Those not specified in the embodiments are carried out according to conventional conditions or conditions recommended by the manufacturer. Those reagents or instruments not specified by the manufacturer can be obtained as conventional products through commercial purchase.

[0058] The tannic acid is dark red or brown granules, odorless, slightly having a special smell, extremely astringent in taste, soluble in water and ethanol, easily soluble in glycerol, and almost insoluble in ether, chloroform, and its benzene molecular formula is C 76 H 52 O 46 .

[0059] The features and properties of the present invention will be further described in detail below in combination with the embodiments.

[0060] Example 1

[0061] A preparation method of a novel stomach - passing and intestine - sustained - release tannic acid includes the following steps:

[0062] (1) Preparation of the coating solution: Add 100 g of glyceryl succinate, 100 g of sodium stearoyl lactate, 100 g of D-mannitol, 20 g of hydroxypropyl starch, and 100 g of polyacrylic resin to 1600 g of water and stir to dissolve completely; add 1200 g of melted hydrogenated palm oil and stir for 10 minutes to disperse evenly; then emulsify for 45 minutes to obtain the coating solution (keep it at 60 - 70 °C for standby. The same below).

[0063] (2) Feed the coating solution prepared in step (1) and 3800 g of tannic acid particles simultaneously into the FLP 5 type coating fluidized bed of Changzhou Jiafa Granulation and Drying Equipment Co., Ltd., maintain the inlet air temperature at 50 ± 5 °C, the outlet air temperature at 35 ± 5 °C; the wind speed at 30 Hz, coat for 2 hours, then discharge and screen to obtain a product that passes through a 20-mesh sieve by 100% and a 80-mesh sieve by less than 5%, that is, tannic acid capable of passing through the stomach and intestines with slow release (denoted as coated tannic acid).

[0064] The preparation method of the tannic acid particles in step (2) includes the following steps:

[0065] First, weigh 70 kg of tannic acid and 30 kg of binder (composed of 80 w / w% corn starch, 10 w / w% microcrystalline cellulose, and 10 w / w% talc powder), put them into the hopper of the HWMG200 type high-efficiency wet granulation machine, extrude the material with a low-temperature extruder at a rotation speed of 40 rpm and an aperture of 0.6 mm, and then round the tannic acid particles with a rounding machine with a rolling disc diameter of 700 mm at a rotation speed of 300 r / min to make tannic acid particles.

[0066] Example 2

[0067] A preparation method of a new type of tannic acid with slow release through the stomach and intestines, including the following steps:

[0068] (1) Preparation of the coating solution: Add 100 g of glyceryl mono / dilaurate, 100 g of sorbitan monostearate, 100 g of maltitol, 20 g of octenyl succinate distarch phosphate, and 100 g of polyacrylic resin to 1600 g of water and stir to dissolve completely; add 1200 g of melted hydrogenated palm oil and stir for 10 minutes to disperse evenly, then emulsify for 60 minutes to obtain the coating solution;

[0069] (2) The coating solution prepared in step (1) and 3,800 grams of tannic acid particles (prepared in the same way as in Example 1, the same below) are simultaneously fed into the FLP 5 type coating fluidized bed of Changzhou Jiafa Granulation and Drying Equipment Co., Ltd. The inlet air temperature is maintained at 50 ± 5 °C, and the outlet air temperature is at 35 ± 5 °C; the wind speed is at 36 Hz. Coating is carried out for 2 hours, then the material is discharged and sieved to obtain a product with 100% passing through a 20-mesh sieve and less than 5% passing through an 80-mesh sieve, that is, tannic acid capable of passing through the stomach and intestines with sustained release is obtained (denoted as coated tannic acid).

[0070] Example 3

[0071] A preparation method of a novel tannic acid capable of passing through the stomach and intestines with sustained release, comprising the following steps:

[0072] (1) Preparation of the coating solution: 100 grams of glycerol mono / dilaurate, 100 grams of ammonium phosphatide, 120 grams of soluble soybean polysaccharide, 17 grams of acid-resistant carboxymethyl cellulose sodium, and 150 grams of polyacrylic resin are added to 1,700 grams of water and stirred until dissolved completely; 1,200 grams of melted hydrogenated palm oil is added and stirred for 10 minutes to make it disperse evenly, and then emulsified for 60 minutes to prepare the coating solution;

[0073] (2) The coating solution prepared in step (1) and 3,900 grams of tannic acid particles are simultaneously fed into the FLP 5 type coating fluidized bed of Changzhou Jiafa Granulation and Drying Equipment Co., Ltd. The inlet air temperature is maintained at 50 ± 5 °C, and the outlet air temperature is at 35 ± 5 °C; the wind speed is at 36 Hz. Coating is carried out for 2 hours, then the material is discharged and sieved to obtain a product with 100% passing through a 20-mesh sieve and less than 5% passing through an 80-mesh sieve, that is, tannic acid capable of passing through the stomach and intestines with sustained release is obtained (denoted as coated tannic acid).

[0074] Example 4

[0075] A preparation method of a novel tannic acid capable of passing through the stomach and intestines with sustained release, comprising the following steps:

[0076] (1) Preparation of the coating solution: 100 grams of D-mannitol, 20 grams of hydroxypropyl starch, and 100 grams of polyacrylic resin are added to 1,600 grams of water and stirred until dissolved completely; 1,200 grams of melted hydrogenated palm oil is added and stirred for 10 minutes to make it disperse evenly, and then emulsified for 45 minutes to prepare the coating solution;

[0077] (2) Feed the coating solution prepared in step (1) and 800 g of tannic acid particles into the FLP 5 type coating fluidized bed of Changzhou Jiafa Granulation and Drying Equipment Co., Ltd. simultaneously, maintain the inlet air temperature at 50 ± 5 °C, the outlet air temperature at 35 ± 5 °C; the wind speed at 30 Hz, coat for 2 hours, then discharge and screen to obtain a product that passes through a 20-mesh sieve by 100% and a product that passes through an 80-mesh sieve by less than 5%, that is, obtain tannic acid that can be slowly released in the stomach and intestine (denoted as coated tannic acid).

[0078] Example 5

[0079] A preparation method of a novel tannic acid with slow release in the stomach and intestine, comprising the following steps:

[0080] (1) Preparation of the coating solution: Add 100 g of glyceryl succinate, 100 g of sodium stearoyl lactate, 20 g of hydroxypropyl starch and 100 g of polyacrylic resin to 1600 g of water and stir to dissolve completely; add 1200 g of melted hydrogenated palm oil and stir for 10 minutes to disperse evenly, then emulsify for 45 minutes to prepare the coating solution;

[0081] (2) Feed the coating solution prepared in step (1) and 3800 g of tannic acid particles into the FLP 5 type coating fluidized bed of Changzhou Jiafa Granulation and Drying Equipment Co., Ltd. simultaneously, maintain the inlet air temperature at 50 ± 5 °C, the outlet air temperature at 35 ± 5 °C; the wind speed at 30 Hz, coat for 2 hours, then discharge and screen to obtain a product that passes through a 20-mesh sieve by 100% and a product that passes through an 80-mesh sieve by less than 5%, that is, obtain tannic acid that can be slowly released in the stomach and intestine (denoted as coated tannic acid).

[0082] Example 6

[0083] A preparation method of a novel tannic acid with slow release in the stomach and intestine, comprising the following steps:

[0084] (1) Preparation of the coating solution: Add 100 g of maltitol, 20 g of octenyl succinate distarch phosphate and 100 g of polyacrylic resin to 1600 g of water and stir to dissolve completely. After complete dissolution, add 1200 g of melted hydrogenated palm oil and stir for 10 minutes to disperse evenly, then emulsify for 60 minutes to prepare the coating solution;

[0085] (2) Feed the coating solution prepared in step (1) and 3800 g of tannic acid particles into the FLP 5 type coating fluidized bed of Changzhou Jiafa Granulation and Drying Equipment Co., Ltd. simultaneously, maintain the inlet air temperature at 50 ± 5 °C, the outlet air temperature at 35 ± 5 °C; the wind speed at 36 Hz, coat for 2 hours, then discharge and screen to obtain a product that passes through a 20-mesh sieve by 100% and a product that passes through an 80-mesh sieve by less than 5%, that is, obtain tannic acid that can be slowly released in the stomach and intestine (denoted as coated tannic acid).

[0086] Example 7

[0087] A preparation method of a novel gastric- and intestinal-passable sustained-release tannic acid, comprising the following steps:

[0088] (1) Preparation of the coating solution: Add 100 g of glycerol monolaurate / dilaurate, 100 g of sorbitan monostearate, 20 g of octenyl succinic acid distarch phosphate, and 100 g of polyacrylic resin into 1600 g of water, stir to dissolve completely; add 1200 g of melted hydrogenated palm oil, stir for 10 minutes to disperse evenly, and then emulsify for 60 minutes to obtain the coating solution;

[0089] (2) Feed the coating solution prepared in step (1) and 3800 g of tannic acid particles into the FLP 5 type coating fluidized bed of Changzhou Jiafa Granulation and Drying Equipment Co., Ltd. simultaneously, maintain the inlet air temperature at 50 ± 5 °C, the outlet air temperature at 35 ± 5 °C; the wind speed at 36 Hz, coat for 2 hours, then discharge and screen to obtain a product with 100% passing through a 20-mesh sieve and less than 5% passing through an 80-mesh sieve, that is, the tannic acid capable of gastric- and intestinal-passable sustained release (denoted as coated tannic acid).

[0090] Example 8

[0091] A preparation method of a novel gastric- and intestinal-passable sustained-release tannic acid, comprising the following steps:

[0092] (1) Preparation of the coating solution: Add 120 g of soluble soybean polysaccharide, 17 g of acid-resistant carboxymethylcellulose sodium, and 150 g of polyacrylic resin into 1700 g of water, stir to dissolve completely; add 1200 g of melted hydrogenated palm oil, stir for 10 minutes to disperse evenly, and then emulsify for 60 minutes to obtain the coating solution;

[0093] (2) Feed the coating solution prepared in step (1) and 3900 g of tannic acid particles into the FLP 5 type coating fluidized bed of Changzhou Jiafa Granulation and Drying Equipment Co., Ltd. simultaneously, maintain the inlet air temperature at 50 ± 5 °C, the outlet air temperature at 35 ± 5 °C; the wind speed at 36 Hz, coat for 2 hours, then discharge and screen to obtain a product with 100% passing through a 20-mesh sieve and less than 5% passing through an 80-mesh sieve, that is, the tannic acid capable of gastric- and intestinal-passable sustained release (denoted as coated tannic acid).

[0094] Example 9

[0095] A preparation method of a novel gastric- and intestinal-passable sustained-release tannic acid, comprising the following steps:

[0096] (1) Preparation of the coating solution: Add 100 g of glycerol monolaurate / dilaurate, 100 g of ammonium phosphatide, 17 g of acid-resistant carboxymethyl cellulose sodium, and 150 g of polyacrylic resin into 1700 g of water, stir to dissolve completely; add 1200 g of hydrogenated palm oil melted by heating, stir for 10 minutes to disperse evenly, and then emulsify for 60 minutes to prepare the coating solution;

[0097] (2) Feed the coating solution prepared in step (1) and 3900 g of tannic acid particles into the FLP 5 type coating fluidized bed of Changzhou Jiafa Granulation and Drying Equipment Co., Ltd. simultaneously, maintain the inlet air temperature at 50 ± 5 °C, the outlet air temperature at 35 ± 5 °C; the wind speed at 36 Hz, coat for 2 hours, then discharge and screen to obtain a product that passes through a 20-mesh sieve by 100% and a product that passes through an 80-mesh sieve by less than 5%, that is, obtain tannic acid that can be slowly released through the stomach and intestines (denoted as coated tannic acid).

[0098] Example 10

[0099] A preparation method of a novel tannic acid that can be slowly released through the stomach and intestines, comprising the following steps:

[0100] (1) Preparation of the coating solution: Add 20 g of hydroxypropyl starch and 100 g of polyacrylic resin into 1600 g of water, stir to dissolve completely; add 1200 g of hydrogenated palm oil melted by heating, stir for 10 minutes to disperse evenly, and then emulsify for 45 minutes to prepare the coating solution;

[0101] (2) Feed the coating solution prepared in step (1) and 3800 g of tannic acid particles into the FLP 5 type coating fluidized bed of Changzhou Jiafa Granulation and Drying Equipment Co., Ltd. simultaneously, maintain the inlet air temperature at 50 ± 5 °C, the outlet air temperature at 35 ± 5 °C; the wind speed at 30 Hz, coat for 2 hours, then discharge and screen to obtain a product that passes through a 20-mesh sieve by 100% and a product that passes through an 80-mesh sieve by less than 5%, that is, obtain tannic acid that can be slowly released through the stomach and intestines (denoted as coated tannic acid).

[0102] Example 11

[0103] A preparation method of a novel tannic acid that can be slowly released through the stomach and intestines, comprising the following steps:

[0104] (1) Preparation of the coating solution: Add 20 g of octenyl succinic acid distarch phosphate and 100 g of polyacrylic resin into 1600 g of water, stir to dissolve completely; add 1200 g of hydrogenated palm oil melted by heating, stir for 10 minutes to disperse evenly, and then emulsify for 60 minutes to prepare the coating solution;

[0105] (2) The coating solution prepared in step (1) and 3,800 grams of tannic acid particles are simultaneously fed into the FLP 5 type coating fluidized bed of Changzhou Jiafa Granulation and Drying Equipment Co., Ltd. The inlet air temperature is maintained at 50 ± 5 °C, and the outlet air temperature is at 35 ± 5 °C; the wind speed is at 36 Hz. Coating is carried out for 2 hours, and then the material is discharged and sieved to obtain a product that passes 100% through a 20-mesh sieve and less than 5% through an 80-mesh sieve, that is, tannic acid capable of passing through the stomach and intestines with slow release (denoted as coated tannic acid) is obtained.

[0106] Example 12

[0107] A preparation method of a novel tannic acid with slow release through the stomach and intestines, comprising the following steps:

[0108] (1) Preparation of the coating solution: Add 17 grams of acid-resistant carboxymethylcellulose sodium and 150 grams of polyacrylic resin to 1,700 grams of water, stir to dissolve completely; add 1,200 grams of melted hydrogenated palm oil, stir for 10 minutes to make it disperse evenly, and then emulsify for 60 minutes to prepare the coating solution;

[0109] (2) The coating solution prepared in step (1) and 3,900 grams of tannic acid particles are simultaneously fed into the FLP 5 type coating fluidized bed of Changzhou Jiafa Granulation and Drying Equipment Co., Ltd. The inlet air temperature is maintained at 50 ± 5 °C, and the outlet air temperature is at 35 ± 5 °C; the wind speed is at 36 Hz. Coating is carried out for 2 hours, and then the material is discharged and sieved to obtain a product that passes 100% through a 20-mesh sieve and less than 5% through an 80-mesh sieve, that is, tannic acid capable of passing through the stomach and intestines with slow release (denoted as coated tannic acid) is obtained.

[0110] Effect Example

[0111] 1. Test on the release and absorption effects of coated tannic acid in the animal intestine

[0112] The test process is as follows:

[0113] (1) Preparation of simulated gastric juice and intestinal juice: Prepared according to the pharmacopoeia method.

[0114] (2) Experimental methods for the simulated gastric juice and simulated intestinal juice experimental groups: Take 1 g of each sample (coated tannic acid prepared in Examples 1 to 3 and tannic acid in Example 1 of Chinese Invention Patent CN 107125440A) and add them to 100 mL of simulated gastric juice with a pH of 3.2 and simulated intestinal juice with a pH of 6.2 respectively, dissolve fully, place them in a 37 °C, 150 r / min incubator and oscillate respectively. According to the test design time, take out the liquid and measure the tannic acid content therein (measurement method: NYSL-1006-2022), and calculate the dissolution rate of tannic acid. Repeat the test three times and take the average value.

[0115] The results are shown in Table 1. The coated tannic acid samples prepared in Examples 1-3 of the present invention have a better intestinal sustained-release effect compared with the blank group and CN107125540A, and can better pass through the stomach without being destroyed.

[0116] Table 1 Release rate (%) of each sample in artificial gastric and intestinal fluids

[0117]

[0118] 2. High-temperature resistance test of coated tannic acid

[0119] The test process is as follows:

[0120] (1) Take samples (coated tannic acid prepared in Examples 4-6, tannic acid in Example 1 of Chinese Patent CN 107125440 A, and tannin powder) and compare the release rates after 5 minutes at 60 °C, 70 °C, 80 °C, and 90 °C.

[0121] (2) Test design: Weigh 1 g each of the coated tannic acid prepared in Examples 4-6, tannic acid in Chinese Patent CN107125540A, and tannin powder, place them in 10 mL aqueous solutions at different temperatures, wait for 5 minutes, stir for 10 seconds, and measure the release rate of tannin in the aqueous solution to determine the tolerance of the tannin products of the examples to high-temperature hot water.

[0122] The results are shown in Table 2. The coated tannic acid samples prepared in Examples 4-6 of the present invention have the characteristic of high-temperature resistance and a lower release rate at high temperatures compared with the tannin powder group and CN107125540A.

[0123] Table 2 Tannin release rates (%) of Examples 4, 5, and 6 at different temperatures

[0124]

[0125]

[0126] 3. Antioxidant test of coated tannic acid products

[0127] The test process is as follows:

[0128] (1) Tannic acid has antioxidant properties, but it will also be oxidized when in contact with air. The coating material can isolate the contact between tannic acid and air, maintain its antioxidant properties, and effectively maintaining the antioxidant properties of tannic acid is beneficial for the coated tannin products to play corresponding roles in the animal digestive tract.

[0129] (2) Experimental design: Weigh 10 g each of the coated tannic acid, tannin powder prepared in Examples 7 - 9, and the coated tannic acid in Chinese Patent CN107125540A, expose them to air for 3, 5, 7, 10, and 30 days to allow the products to fully contact with air, and measure the effective content of tannin at 3, 5, 7, 10, and 30 days to confirm the isolation effect of the coating material on tannic acid. The antioxidant performance of the coating material is ultimately reflected in the effective content of tannic acid.

[0130] As shown in Table 3, compared with CN107125540A, the coated tannic acid samples prepared in Examples 7 - 9 of the present invention have better antioxidant performance and can better maintain the effective content of the coated tannic acid.

[0131] Table 3 Effective content of tannin at different time points exposed to air

[0132]

[0133] 4. Experimental test on the growth performance of weaned piglets

[0134] Experimental design and grouping: Select 135 weaned piglets with similar ages and good health, randomly divide them into 3 treatments, with 3 replicates for each treatment and 15 piglets for each replicate. According to the experimental design (see Table 4), each group uses the corresponding experimental diet for a period of 19 days, and each group is weighed before and after the experiment. The basal diet is a corn - soybean meal - based basal diet, and its composition and nutritional value are shown in Table 5.

[0135] Table 4 Experimental design and grouping

[0136] Group Addition level in basal diet Treatment group 1 2000 g / t conventional zinc oxide Treatment group 2 1500 g / t coated tannic acid of Example 1 Treatment group 3 1500 g / t coated tannic acid of Example 2

[0137] Table 5 Basal diet

[0138]

[0139] Note: The premix provides per kg of diet: V A 1200 IU; V D3 4800 IU; V C 200 mg; V E 205 mg; V K 3.6 mg; V B1 3.6 mg; V B2 12 mg; V B6 7.2 mg; V B120.048 mg; pantothenic acid 30.0 mg; niacin 48.0 mg; folic acid 8.6 mg; biotin 0.6 mg; copper 10.0 mg; magnesium 60 mg; manganese 45 mg; cobalt 0.1 mg; wheat bran 2.28 kg. (Composition of the premix) The nutritional value was calculated based on the nutrient concentration of the diet calculated from the nutrient composition of the raw materials in the NRC (2012) database.

[0140] Test site and time: The test was conducted in a test pig farm in Guangdong.

[0141] Feeding and management: During the test period, the daily management of the test pig house was carried out according to the regular procedures of the pig farm. The feeding method of free access to feed and water was adopted. Keep the pig house clean and hygienic, and the ventilation condition is good. During the test period, the diseases, deaths, etc. of the piglets were observed and recorded regularly every day.

[0142] Determination of test indicators:

[0143] 1) Growth performance: Record the feed intake, the number and weight of dead and culled pigs every day, and calculate the average daily gain, daily feed consumption, feed-to-gain ratio and culling rate at each stage during the test period.

[0144] Daily feed intake = total daily addition - remaining feed in the feeder trough per day;

[0145] Average daily feed intake = (total addition - remaining feed in the feeder trough) / (remaining number of pigs in the pen × total number of test days + number of dead and culled pigs × number of test days);

[0146] Average daily gain = (weight at the end of the pen - weight at the beginning of the pen + weight at the end of dead and culled pigs) / (remaining number of pigs in the pen × total number of test days + number of dead and culled pigs × number of test days);

[0147] Feed-to-gain ratio = total feed intake / total weight gain;

[0148] Culling rate = (total number of dead and culled pigs × 100%) / total number of piglets in each group.

[0149] 2) Diarrhea rate

[0150] According to the diarrhea scoring standard (Table 6), record the diarrhea situation of the piglets every day.

[0151] Diarrhea rate = total number of diarrhea cases × 100% / (total number of test pigs × total number of test days)

[0152] Table 6 Diarrhea scoring standard

[0153] Score Degree Fecal appearance Moisture content in feces (%) 0 Normal (no diarrhea) Striped or granular <70 1 Mild diarrhea Soft feces, can form 70～75 2 Moderate diarrhea Thick, unformed, feces and water not separated 75～80 3 Severe diarrhea Liquid, unformed, feces and water separated >80

[0154] Data statistics and analysis: The test data were sorted out using Excel, and one-way analysis of variance was performed using SPSS statistical software. Among them, P > 0.05 indicates no significant difference, P < 0.05 indicates a significant difference, and P < 0.01 indicates a highly significant difference.

[0155] Results and analysis: The experimental results are shown in Table 7. The final weights, total weight gains, average daily weight gains, and feed intakes of the piglets in Treatment Group 2 and Treatment Group 3 were significantly better than those of the other two treatment groups (P < 0.05); the feed-to-gain ratios of the piglets in Treatment Group 2 and Treatment Group 3 were significantly lower than that in Treatment Group 1 (P < 0.05); the diarrhea rates of the piglets in Treatment Group 2 and Treatment Group 3 were significantly lower than that in Treatment Group 1.

[0156] Results description: In the basal diet at the weaning stage, compared with 2000 g / t of conventional zinc oxide, adding 1500 g / t of the coated tannic acid prepared in Examples 1-2 could improve weight gain, improve the feed-to-gain ratio, reduce the diarrhea rate, and significantly improve the production performance of weaned piglets. Therefore, replacing part of the zinc oxide with coated tannic acid could significantly improve the production performance of weaned piglets, increase weight gain, reduce the feed-to-gain ratio, and reduce diarrhea.

[0157] Table 7 Effects of Different Types of Tannic Acid Products on the Growth Performance of Weaned Piglets

[0158] Item Treatment group 1 Treatment group 2 Treatment group 3 Initial weight (kg) 7.78±0.37 7.78±0.30 7.78±0.11 Final weight (kg) 13.03±0.97b 14.88±0.44a 14.35±0.38b Total weight gain (kg) 5.35±0.80b 7.10±0.34a 6.57±0.39b Average daily weight gain (g) 282±42b 374±18a 345±19b Total feed intake (kg) 10.52±1.36 10.46±0.46 10.43±0.73 Average daily feed intake (g) 553±71 551±24 548±39 Feed to meat ratio 1.92±0.004b 1.48±0.007a 1.58±0.002b Diarrhea rate (%) 3.97 2.78 2.55 Fecal score 1.06 1 1.25

[0159] Note: Data with the same superscript letter in the same column / row indicate no significant difference (P > 0.05), and different lowercase letters indicate significant difference (P < 0.05).

[0160] The above has described the embodiments of the present invention in detail. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those of ordinary skill in the art, various changes can be made without departing from the purpose of the present invention. In addition, the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

Claims

1. A gastric-passage and enteric-coated material, comprising a surfactant, a sugar alcohol, a hydrogenated oil, a polyacrylic resin, and a thickener.

2. The gastric-passage and enteric-coated material according to claim 1, wherein, The surfactant includes at least one of ammonium phospholipid, mono / diglycerol fatty acid ester, glycerol succinate, polyglycerol ricinoleate, polyglycerol fatty acid ester, polyoxyethylene xylitan monostearate, Tween, xylitan monostearate, citric acid fatty acid glycerol ester, hydrogenated rosin glycerol ester, lactic acid fatty acid glycerol ester, Span, diacetyl tartaric acid mono / diglyceride, sulfosuccinate, fatty acid sulfonate, and sodium stearoyl lactate; and / or, the thickener includes at least one of octenyl succinic acid distarch phosphate, propylene glycol ester, antacid carboxymethyl cellulose sodium, hydroxypropyl starch, pectin, xanthan gum, alginate, carrageenan, agar, modified starch, guar gum, locust bean gum, konjac gum, pectin, low-fat pectin, gum arabic, and sodium polyacrylate.

3. The stomach-passing and enteric-coated material according to claim 1, characterized in that, The sugar alcohol includes at least one of propylene glycol alginate, mannitol, pectin, soluble soybean polysaccharide, maltitol, lactitol, and sorbitol; and / or, the hydrogenated oil includes at least one of hydrogenated palm oil, hydrogenated rapeseed oil, hydrogenated soybean oil, hydrogenated coconut oil, hydrogenated castor oil, palm stearin, hydrogenated peanut oil, and hydrogenated corn oil.

4. The gastric-passage and enteric-coated material according to any one of claims 1 to 3, characterized in that, By mass, the gastric-passage and enteric-coated material includes 100 - 300 parts of surfactant, 80 - 150 parts of sugar alcohol, 1000 - 1500 parts of hydrogenated oil, 80 - 130 parts of polyacrylic resin, and 10 - 30 parts of thickener.

5. A coated tannic acid, comprising the gastric-passage and enteric-coated material according to any one of claims 1 - 4 and tannic acid particles.

6. The encapsulated tannic acid according to claim 5, wherein The mass ratio of the gastric-passage and enteric-coated material to the tannic acid particles in the coated tannic acid is 1:1 - 3.

7. The coated tannic acid according to claim 6, characterized in that, The tannic acid particles include tannic acid and a binder; Preferably, the method for preparing the tannic acid particles includes mixing tannic acid with a binder and granulating to obtain the tannic acid particles.

8. The preparation method of the tannic acid-coated according to any one of claims 5 to 7, comprising the following steps: The gastric-passage and enteric-coated material and the tannic acid particles are mixed and coated in a coating fluidized bed to obtain the coated tannic acid.

9. Use of the coated tannic acid according to any one of claims 5 - 7 in the preparation of a feed or a feed additive.

10. A feed or a feed additive, comprising the coated tannic acid according to any one of claims 5 - 7.

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