A ceramide composition for promoting cell repair and a preparation method and application thereof

Abstract

The application discloses a ceramide composition capable of promoting cell repair and a preparation method and application thereof, and belongs to the technical field of food, wherein the ceramide composition is composed of the following raw materials in parts by weight: 0.5-2 parts of rice bran oil powder, 0.5-2 parts of salvia extract and 5-32 parts of bioactive peptides; the bioactive peptides are fish collagen peptide, bird's nest peptide and sea cucumber peptide, and the weight ratio of the fish collagen peptide, the bird's nest peptide and the sea cucumber peptide is 30-55:1:1-10. Through the synergistic effect among the rice bran oil powder, the salvia extract and the bioactive peptides, the application promotes cell repair together, and realizes efficient repair of a skin barrier.

Description

Technical Field

[0001] The present invention belongs to the field of food technology, and specifically relates to a ceramide composition capable of promoting cell repair, and a preparation method and application thereof. Background Art

[0002] As the largest organ in the human body, the skin possesses a barrier function and multiple immune functions. The skin barrier plays a crucial role in preventing water loss, resisting external stimuli, and protecting the skin from infection. In a narrow sense, the skin barrier refers to a physiological structure in the surface layer of the skin, primarily composed of keratinocytes, intercellular lipids, desmosomes, and tight junctions. An intact skin barrier structure is fundamental to the body's physiological responses. When the skin barrier is damaged, the skin's protective function is weakened, making it more susceptible to external factors and potentially inducing and exacerbating a range of skin problems.

[0003] As a key active ingredient in intercellular lipids, ceramide plays an irreplaceable role in promoting cell repair. It not only participates in the construction of intercellular lipids and maintains the tightness of the skin barrier, but also regulates the proliferation, differentiation, and apoptosis of keratinocytes. When the skin barrier is damaged, ceramide promotes the repair of damaged cells, accelerates cell metabolism, and helps cells restore normal physiological functions, thereby repairing and strengthening the skin barrier at the cellular level.

[0004] Patent CN120000561A discloses a barrier-repairing composition containing red pine leaf extract and olive ceramide NPs, while patent CN117860631A discloses a skincare composition containing avocado resin, ceramide NPs, hyaluronic acid, and bisabolol. However, both technologies are intended for topical application only. However, topical ceramides have poor transdermal absorption, and the dense structure of the skin's stratum corneum limits ceramide penetration, making it difficult for a single ceramide subtype to achieve a repairing effect. Furthermore, topical ceramides are mostly short-term repairs, lacking mechanisms for sustained release or stimulation of endogenous ceramide synthesis, making it difficult to maintain homeostasis in the skin's barrier structure and function.

[0005] Most solutions for repairing the skin barrier have highly homogenized ingredients and lack proven comprehensive synergistic effects. For example, patent CN117659118A discloses a collagen tripeptide that promotes the proliferation of skin keratinocytes. This invention promotes skin barrier repair to a certain extent by preparing collagen peptides with special peptide segments, but lacks a multi-target synergistic design. Summary of the Invention

[0006] To solve the above technical problems, the present invention provides a ceramide composition that can promote cell repair, as well as its preparation method and application. The ceramide composition promotes cell repair through the synergistic effect between the raw materials, thereby achieving effective repair of the skin barrier.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a ceramide composition capable of promoting cell repair, which is composed of the following raw materials in parts by weight: 0.5-2 parts of rice bran oil powder, 0.5-2 parts of sage extract, and 5-32 parts of bioactive peptides.

[0009] The rice bran oil powder in this invention contains oryzanol, phytosterols, and ceramide analogs, which can strengthen cell membrane structure, protect against free radical damage, and provide cellular nutrition. Sage extract, with its components like rosmarinic acid, potently scavenges reactive oxygen species, inhibits inflammatory signaling pathways, and creates a stable cellular microenvironment. Bioactive peptides can reshape the extracellular matrix and activate cell proliferation and repair signaling pathways. These three ingredients work synergistically across multiple targets to promote cell repair.

[0010] In some embodiments, the ceramide composition is composed of the following raw materials in parts by weight: 1-1.5 parts of rice bran oil powder, 0.5-1 part of sage extract, and 10-27 parts of bioactive peptides.

[0011] In some embodiments, the ceramide composition is composed of the following raw materials in parts by weight: 1.2 parts of rice bran oil powder, 0.8 parts of sage extract, and 15 parts of bioactive peptides.

[0012] In some embodiments, the bioactive peptides are fish collagen peptides, bird's nest peptides, and sea cucumber peptides. Preferably, the weight ratio of the fish collagen peptides, bird's nest peptides, and sea cucumber peptides is 30-55:1:1-10; more preferably 35-50:1:3-8; and most preferably 40:1:5.

[0013] In some embodiments, the mass percentage of ceramide in the rice bran oil powder is 1%-3%.

[0014] In some embodiments, the fish collagen peptide has a molecular weight of 1000-5000 Da.

[0015] In a second aspect, the present invention provides a method for preparing the above-mentioned ceramide composition, comprising the following steps: mixing rice bran oil powder, sage extract and bioactive peptides.

[0016] The beneficial effects of the present invention are:

[0017] 1. This invention promotes cell repair through the synergistic effects of rice bran oil powder, sage extract, and bioactive peptides, achieving efficient repair of the skin barrier. Rice bran oil powder contains oryzanol, phytosterols, and ceramide analogs, which can strengthen cell membrane structure, protect against free radical damage, and provide nutrition to cells. Sage extract, with its components such as rosmarinic acid, potently scavenges reactive oxygen species, inhibits inflammatory signaling pathways, and creates a stable cellular microenvironment. Bioactive peptides reshape the extracellular matrix and activate cell proliferation and repair signaling pathways. The three ingredients work synergistically at multiple targets to promote cell repair.

[0018] 2. The plant-derived ceramides contained in the ceramide composition of this invention are greener, safer, and more bioavailable due to their natural properties and skin affinity. Compared to animal-derived or biosynthetic ceramides, they are milder and less irritating, making them more suitable for repairing sensitive skin or damaged skin barrier.

[0019] 3. The ceramide used in this invention is derived from rice bran oil, a byproduct of rice processing. Due to its rich fatty acid content, it has become a high-quality oil resource in my country. By integrating and efficiently utilizing resources, raw material costs can be reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 These are scratch images of the Hacat cell surfaces in different groups.

[0021] Figure 2 The figure shows the statistical graph of Hacat cell migration rate after treatment with different groups. Compared with the blank control group, ***P<0.001, ****P<0.0001, ns means there is no significant difference compared with the blank control group, P>0.05. DETAILED DESCRIPTION

[0022] The description of the following embodiments is only intended to help understand the method of the present invention and its core ideas. It should be noted that for those skilled in the art, without departing from the principles of the present invention, several improvements and modifications may be made to the present invention, and these improvements and modifications also fall within the scope of protection of the claims of the present invention. The following description of the disclosed embodiments enables professionals in this field to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but may be applied to a wider range consistent with the principles and novel features disclosed herein. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present invention belongs.

[0023] The following are examples and comparative examples of the present invention to describe the liquid beverage of the present invention in more detail, but the present invention is not limited thereto. Unless otherwise specified, all percentages in the present invention are by mass.

[0024] The present invention does not limit the sources of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are common commercial products in the technical field.

[0025] The rice bran oil powder described in the present invention is a conventional food in the art and can be prepared by conventional methods in the art. For example, rice bran oil is extracted, filtered, diluted with dextrin, pasteurized, and spray-dried to obtain rice bran oil powder. The fish collagen peptide meets the requirements of SB / T 10634-2011 freshwater fish collagen peptide powder and can be prepared by conventional methods in the art. For example, fish scales / fish skin are cleaned, enzymatically hydrolyzed, filtered, decolorized and deodorized, concentrated, sterilized, and spray-dried to obtain fish collagen peptide powder.

[0026] The following sources of raw materials are illustrative: rice bran oil powder, purchased from Hongcheng (Shanghai) Health Technology Co., Ltd.; sage extract, purchased from Guangzhou Kanglai Food Additive Co., Ltd.; fish collagen peptide, purchased from Hubei Ruibang Biotechnology Co., Ltd., with an average molecular weight of approximately 1000 Da; bird's nest peptide, purchased from Hubei Ruibang Biotechnology Co., Ltd.; sea cucumber peptide, purchased from Zhejiang Nuo Derivative Technology Co., Ltd.

[0027] Examples 1-5 and Comparative Examples 1-3

[0028] The ceramide composition is prepared by uniformly mixing the raw materials in the formula amounts shown in Table 1.

[0029] Table 1

[0030]

[0031] Note: 1. The units of dosage in the table are parts by weight; 2. A:B:C in the table is the weight ratio of fish collagen peptide, bird's nest peptide and sea cucumber peptide; 3. " / " in the table means the dosage is 0.

[0032] Hacat cell scratch migration assay

[0033] a) Seed Hacat cell suspension in a 12-well plate and culture in a 37°C, 5% CO2 incubator until the cell density reaches 95%-100%.

[0034] b) starting scarification and administering the drug after the cell density reaches 95%-100%. Before scarification, the compositions of the Examples and Comparative Examples were prepared using serum-free MEM medium, and microbial filtration was performed to remove the microorganisms in the sample to avoid contamination.

[0035] c) Discard the old culture medium and use the tip of a 200 μL pipette to create three vertical scratches perpendicular to the cell wells.

[0036] d) Wash with PBS 1-2 times to remove cell debris generated by scratching;

[0037] e) Place the cell plate under a microscope and observe and photograph the initial scratch;

[0038] f) The experiment was set up with a blank control group, a positive control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group;

[0039] g) 1 mL of serum-free MEM medium was added to the first well of the first column of a 12-well plate to serve as a blank control group; 1 mL of serum-containing MEM medium was added to the second well of the second column to serve as a positive control group; and 1 mL of the prepared composition of each Example and Comparative Example was added to each of the remaining wells to serve as each Example group and Comparative Example group;

[0040] h) After drug addition, the cells were placed in a 37°C, 5% CO2 incubator for 24 hours;

[0041] i) Aspirate the culture medium from each well and observe and photograph the scratch marks under a microscope 24 hours after drug addition.

[0042] Image J software was used to analyze the width of each scratch and calculate the scratch migration rate. Scratch migration rate = (S0H-S24H) / S0H*100%, where S0H is the scratch length at 0 hours and S24H is the scratch length at 24 hours.

[0043] By scratching the cell surface to create a wound, the changes in the scratch width after 24 hours were compared to explore the effects of each treatment group on the migration ability of Hacat cells. The results are as follows Figure 1 and Figure 2 shown.

[0044] The results showed that compared with the blank control group, the migration rates of Hacat cells in Example 1 to Example 5 groups were significantly different (P < 0.05), indicating that the ceramide compositions prepared in Examples 1 to 5 can significantly increase the migration rate of Hacat cells and promote cell repair.

[0045] Compared with the blank control group, there was no significant difference in the migration rate of Hacat cells in groups 1 to 3 (P>0.05), indicating that the ceramide compositions prepared in Comparative Examples 1 to 3 were unable to promote Hacat cell migration and cell repair. It can be seen that at the same dosage concentration, the combination of rice bran oil powder and sage extract or the bioactive peptide alone was unable to promote Hacat cell migration. At the same time, the combination of rice bran oil powder, sage extract, and bioactive peptides in a conventional dosage relationship also failed to promote Hacat cell migration. However, the present invention, using a combination of rice bran oil powder, sage extract, and bioactive peptides in a specific dosage relationship, can significantly promote the migration and fusion of Hacat cells, have a good skin barrier repair effect, and achieve unexpected technical results.

[0046] The above further describes the present invention in conjunction with specific embodiments. However, these embodiments are merely exemplary and do not constitute any limitation on the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solutions of the present invention may be modified or replaced without departing from the spirit and scope of the present invention, and such modifications and replacements shall fall within the scope of protection of the present invention.

Claims

1. A ceramide composition that can promote cell repair, characterized in that: The invention is composed of the following raw materials in parts by weight: 0.5-2 parts of rice bran oil powder, 0.5-2 parts of sage extract and 5-32 parts of bioactive peptides; the bioactive peptides are fish collagen peptides, bird's nest peptides and sea cucumber peptides, and the weight ratio of the fish collagen peptides, bird's nest peptides and sea cucumber peptides is 30-55:1:1-10.

2. The ceramide composition according to claim 1, characterized in that The invention is composed of the following raw materials in parts by weight: 1-1.5 parts of rice bran oil powder, 0.5-1 part of sage extract and 10-27 parts of bioactive peptide.

3. The ceramide composition according to claim 1, characterized in that The invention is composed of the following raw materials in parts by weight: 1.2 parts of rice bran oil powder, 0.8 parts of sage extract and 15 parts of bioactive peptides.

4. The ceramide composition according to claim 1, characterized in that The weight ratio of the fish collagen peptide, bird's nest peptide and sea cucumber peptide is 35-50:1:3-8.

5. The ceramide composition according to claim 1, characterized in that The weight ratio of the fish collagen peptide, bird's nest peptide and sea cucumber peptide is 40:1:

5.

6. The ceramide composition according to claim 1, characterized in that The mass percentage of ceramide in the rice bran oil powder is 1%-3%.

7. The ceramide composition according to claim 1, characterized in that The molecular weight of the fish collagen peptide is 1000-5000 Da.

8. The method for preparing the ceramide composition according to any one of claims 1 to 7, characterized in that: The method comprises the following steps: mixing rice bran oil powder, sage extract and bioactive peptides.

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