A biological combination agent for the control of crop pests and its application

Abstract

This invention discloses a biological combination preparation for controlling crop pests and its application, belonging to the field of agricultural biotechnology. The biological combination preparation includes the plant inducer alpha-linolenic acid (ALA) and the pesticide chlorantraniliprole. For application, each 1g of dry seeds is thoroughly mixed with 1mL of an ALA solution at a concentration of 4-400mg / L and 2.5-5mg of a 20% chlorantraniliprole suspension before sowing. This invention is the first to utilize ALA in synergistic seed treatment with chemical pesticides, achieving a high lethality against pests while reducing the amount of chemical pesticides used. The treatment method of this invention does not affect seed germination rate or crop growth and development; it reduces the amount of chemical pesticides used by half compared to conventional seed treatment methods; and it is an intensive, simple, and labor-saving operation with significant social and ecological benefits.

Description

Technical Field

[0001] This invention belongs to the field of agricultural biotechnology, specifically relating to a biological combination preparation for controlling crop pests and its application. Background Art

[0002] Crops inevitably suffer from various pests and diseases during their growth process, and untimely control can lead to losses or even total crop failure. Effective control of crop pests and diseases is crucial for high and stable yields. Currently, crop pest and disease control still relies mainly on foliar spraying, which requires a large labor force. While family farms, large-scale growers, and new-type farmer cooperatives are constantly developing and expanding, the increasing aging population and ongoing urbanization are exacerbating the shortage of rural labor. During crop pest and disease control periods, growers often struggle to find workers; furthermore, frequent extreme heatwaves make spraying operations extremely difficult. Therefore, growers urgently need new technologies for crop pest and disease control that can reduce the number of applications while achieving better control effects. In addition, foliar spraying of pesticides also has disadvantages such as low pesticide utilization, droplet drift posing a safety hazard to humans and livestock, significant non-point source pollution, and substantial impacts on non-target organisms. Developing new pest and disease control technologies that can achieve better control effects while minimizing or avoiding the adverse effects of current foliar spraying methods is a major requirement for the green development of agricultural production.

[0003] Seed treatment can, on the one hand, restore seed viability to a certain extent, thereby increasing germination rate, promoting uniform seedling emergence, and ensuring strong seedlings; on the other hand, it can prevent early crop diseases and pests, thus promoting healthy crop growth and development and increasing yield (Invention Patent Publication No. CN107771811A, Publication Date March 9, 2018; Invention Patent Publication No. CN114903041A, Publication Date August 16, 2022). Disease and pest control technology based on seed treatment with pesticides has already been applied in production. Research by Yu Julong et al. found that treating 1g of dry rice seeds with 5-10mg of 20% chlorantraniliprole suspension resulted in a control effect against rice leaf folder for up to 110 days, reducing the need for two applications. However, the control effect significantly decreased when the dosage was below 5mg (2019, *Journal of Pesticide Science*, paper title "Effect and Safety Evaluation of Chlorantraniliprole Seed Treatment against Rice Leaf Folder"). Developing new seed treatment methods or technologies that can reduce the use of chemical pesticides while still achieving good pest and disease control is crucial for protecting the farmland ecological environment and promoting the green development of agricultural production. However, screening and developing new seed treatment agents and technologies requires extensive exploration and experimentation.

[0004] There are reports on the use of substances in plant defense hormone pathways for the control of crop diseases and pests. Methyl jasmonate is an important substance in plant defense hormone pathways, and a patent (publication number CN111436453A, publication date July 24, 2020) discloses "a compound insecticide for controlling green mirid bugs." This patent mixes the chemical pesticide imidacloprid, methyl jasmonate, and Alternaria alternata protein to control green mirid bugs. Based on this technology, the amount of chemical pesticides used is reduced without reducing the control effect on green mirid bugs, achieving good social and ecological benefits.

[0005] Seed-based crop pest and disease control technology effectively implements the plant protection policy of "prevention first, integrated management." Simultaneously, this technology is simple to operate and intensive, achieving good control results while reducing the number of pesticide applications, making it popular among growers. Furthermore, seed treatment technology largely avoids the problems of low pesticide utilization, significant pesticide runoff leading to environmental pollution, and poisoning of humans and animals caused by traditional pesticide spraying methods. In recent years, there has been a continuous push to reduce the use of chemical pesticides in crop production. For seed treatment technology, how to achieve good control of crop pests and diseases while reducing the amount of chemical pesticides used has always been a focus of attention in the field of plant protection. For pest control in crops such as rice, corn, and cabbage, there is an urgent need to develop green, efficient, simplified, and intensive control technologies. Summary of the Invention

[0006] Objective: To address the problems of high chemical pesticide usage and labor shortages in crop application, this invention provides a biological combination formulation for controlling crop pests, offering important reference for developing green, efficient, simplified, and intensive integrated pest management technologies; significantly reducing the amount of chemical pesticides used for crop pest control; alleviating the labor shortage problem in pesticide application; and reducing non-point source pollution from pesticides in the field. This invention also provides an application method for controlling crop pests.

[0007] Technical solution: In order to achieve the above objectives, the present invention provides a biological combination preparation for controlling crop pests, characterized in that the biological combination preparation includes the plant inducer α-linolenic acid and chlorantraniliprole, wherein the mass ratio of the two is 0.004-0.4:0.5-5.

[0008] The biological combination formulation includes the plant resistance inducer α-linolenic acid and 20% chlorantraniliprole suspension, wherein the mass ratio of the two is 0.004-0.4:2.5-5.

[0009] Preferably, the biological combination formulation comprises the plant resistance inducer α-linolenic acid and 20% chlorantraniliprole suspension, wherein the mass ratio of the two is 0.004-0.4:2.5.

[0010] The crops mentioned are rice, corn, or cabbage, etc.

[0011] The pests mentioned include rice stem borer, rice leaf roller, fall armyworm, corn borer, cotton bollworm, armyworm, diamondback moth, or cabbage caterpillar, etc.

[0012] The application of the biological combination preparation for controlling crop pests described in this invention in the control of crop pests.

[0013] The biological combination agent used to control crop pests is applied to control crop pests through seed dressing.

[0014] The specific process of the application is as follows: seeds are treated with a mixture of plant inducer α-linolenic acid and chlorantraniliprole, and then sown after thorough mixing.

[0015] For each 1g of dry seeds, mix thoroughly with 1mL of α-linolenic acid solution at a concentration of 4-400mg / L and 2.5-5mg of 20% chlorantraniliprole suspension.

[0016] After thorough mixing and seed dressing, the seeds should be placed in a cool place for 12-24 hours before sowing.

[0017] The sowing process involves directly scattering seeds into the field through direct sowing, or first raising seedlings in seedling trays and then transplanting them.

[0018] This invention is the first to propose using α-linolenic acid seed dressing for crop pest and disease control, and further, the first to utilize α-linolenic acid in conjunction with chemical agents for seed dressing to control crop pests and diseases.

[0019] Numerous substances exist in the plant defense hormone pathway, but screening for substances that can enhance crop resistance to pests requires extensive experimental verification; and the usual screening method is pesticide spraying. Currently, it is difficult to obtain substances suitable for seed treatment with good insecticidal effects. This invention has screened a plant-induced resistance substance, "α-linolenic acid," and for the first time clearly demonstrated that this substance can improve crop insect resistance through seed treatment. After treatment, it does not affect seed germination rate, crop growth and development, and can significantly improve crop resistance to pests. Furthermore, this invention is the first to synergistically treat seeds with the resistance inducer α-linolenic acid and chemical pesticides. After treatment, it does not affect seed germination rate, crop growth and development, and still has a good control effect on crop pests while reducing the amount of chemical pesticides used.

[0020] Beneficial effects: Compared with the prior art, the present invention has the following advantages:

[0021] This invention proposes a biological combination formulation for controlling crop pests by mixing alpha-linolenic acid with chemical pesticides. This invention is the first to utilize alpha-linolenic acid and chemical pesticides in synergistic seed treatment, which can significantly reduce the amount of chemical pesticides used (by at least half) while still having a high lethal effect on pests. Furthermore, alpha-linolenic acid is environmentally friendly and human-friendly, significantly reducing the pollution of the environment caused by chemical pesticides.

[0022] This invention utilizes a mixture of a specific concentration of α-linolenic acid and a reduced amount of 20% chlorantraniliprole suspension for seed treatment, achieving good crop pest control while reducing the amount of chemical pesticides used. The biological combination formulation of this invention does not affect seed germination rate or crop growth and development; it reduces the amount of chemical agents used by half compared to conventional seed treatment methods; and it is an intensive, simple, and labor-saving operation with significant social and ecological benefits. Attached Figure Description

[0023] Figure 1 The seedling height of rice seedlings treated with α-linolenic acid in Nanjing 9108 rice seeds was determined by different lowercase letters, which represent significant differences at the P<0.05 level.

[0024] Figure 2 The plant height of rice seedlings after seed treatment with α-linolenic acid in Shenliangyou No. 1 rice seed is determined by different lowercase letters, which represent significant differences at the P<0.05 level.

[0025] Figure 3 The plant height of Nanjing 9108 varietal seed was determined after seed treatment with a mixture of α-linolenic acid and chlorantraniliprole suspension. Different lowercase letters represent significant differences at the P<0.05 level. CK: water control; T1: seed treatment with a mixture of 4 mg / L α-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T2: seed treatment with a mixture of 40 mg / L α-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T3: seed treatment with a mixture of 400 mg / L α-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension.

[0026] Figure 4 To measure the growth of rice stem borer after seed dressing, different lowercase letters represent significant differences at the P<0.05 level. CK: water control;

[0027] Figure 5To test the susceptibility of rice stem borer after seed dressing, (A) Nanjing 9108 rice variety and (B) Shenliangyou 1 rice variety were used. Different lowercase letters represent significant differences at the P<0.05 level. CK: Water control; T1: Seed dressing with a mixture of 4 mg / L α-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T2: Seed dressing with a mixture of 40 mg / L α-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T3: Seed dressing with a mixture of 400 mg / L α-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T4: Seed dressing with 5 mg 20% ​​chlorantraniliprole suspension.

[0028] Figure 6 The seed height of maize after seed treatment is shown. Different lowercase letters represent significant differences at the P<0.05 level. CK: water control; T1: seed treatment with a mixture of 4 mg / L alpha-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T2: seed treatment with a mixture of 40 mg / L alpha-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T3: seed treatment with a mixture of 400 mg / L alpha-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T4: seed treatment with 5 mg 20% ​​chlorantraniliprole suspension.

[0029] Figure 7 To assess the effectiveness of seed treatment for fall armyworm in maize, different lowercase letters represent significant differences at the P<0.05 level. CK: water control; T1: seed treatment with a mixture of 4 mg / L alpha-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T2: seed treatment with a mixture of 40 mg / L alpha-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T3: seed treatment with a mixture of 400 mg / L alpha-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T4: seed treatment with 5 mg 20% ​​chlorantraniliprole suspension.

[0030] Figure 8 The seed height of cabbage after seed treatment was determined by different lowercase letters, with P < 0.05 indicating significant differences. CK: water control; T1: seed treatment with a mixture of 4 mg / L alpha-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T2: seed treatment with a mixture of 40 mg / L alpha-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T3: seed treatment with a mixture of 400 mg / L alpha-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T4: seed treatment with 5 mg 20% ​​chlorantraniliprole suspension.

[0031] Figure 9To assess the effectiveness of seed treatment for diamondback moth in cabbage, different lowercase letters represent significant differences at the P<0.05 level. CK: water control; T1: seed treatment with 40 mg / L alpha-linolenic acid; T2: seed treatment with a mixture of 40 mg / L alpha-linolenic acid and 2.5 mg 20% ​​chlorantraniliprole suspension; T3: seed treatment with 5 mg 20% ​​chlorantraniliprole suspension. Detailed Implementation

[0032] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0033] Unless otherwise specified, all materials and reagents used in the following examples are commercially available. Experimental methods not specifically described in the examples are generally performed under standard conditions or as recommended by the manufacturer.

[0034] The 20% chlorantraniliprole suspension (trade name: Concan, DuPont) was purchased from the market. Alpha-linolenic acid was commercially available and could be used in any form.

[0035] Example 1

[0036] Three concentrations of alpha-linolenic acid (ALA) were set: 4 mg / L, 40 mg / L, and 400 mg / L. Nanjing 9108 (japonica rice) and Shenliangyou 1 (indica rice) were used for seed treatment. Each 1 g of dry seed was treated with 1 mL of different concentrations of ALA solution (4-400 mg / L). The weighed rice seeds were placed in a sealed plastic bag (smaller quantities were used in indoor experiments), and the ALA solution was added. The bag was then vigorously shaken for about 5 minutes to ensure thorough mixing. After being placed in a cool, shaded place for 24 hours, the seeds were sown in petri dishes lined with absorbent paper to maintain moisture. A water treatment was used as a control (CK). Germination rate and germination time were then statistically analyzed.

[0037] The results of the germination rate and germination time measurements of rice seeds showed that the germination rate and germination time of Nanjing 9108 seeds treated with different concentrations of α-linolenic acid fluctuated somewhat compared with the control (CK), but there were no statistically significant differences (Table 1). The germination rate of Shenliangyou 1 seeds treated with different concentrations of α-linolenic acid showed an increasing trend, but there was no significant difference compared with the control (CK); the germination time was significantly shorter at a concentration of 4 mg / L than the control, and slightly longer at concentrations of 40 mg / L and 400 mg / L, but there were no statistically significant differences (Table 2).

[0038] Table 1. Germination rate and germination time of Nanjing 9108 seeds

[0039]

[0040] Note: Different lowercase letters indicate significant differences at the P<0.05 level.

[0041] Table 2 Germination rate and germination time of Shenliangyou No. 1 seeds

[0042]

[0043] Note: Different lowercase letters indicate significant differences at the P<0.05 level.

[0044] The results show that rice seeds treated with different concentrations of alpha-linolenic acid (ALA) generally had no significant adverse effects. In fact, some treatment concentrations showed certain beneficial effects, including increased germination rate and shortened germination time. However, further increases in the concentration of ALA would affect seed germination.

[0045] Example 2

[0046] Three concentration gradients of α-linolenic acid solution were set up: 4 mg / L, 40 mg / L, and 400 mg / L. Nanjing 9108 (japonica rice) and Shenliangyou 1 (indica rice) were used for seed treatment. Water treatment served as the control (CK). The seed dressing treatment method was the same as in Example 1. After treatment, the seeds were sown into disposable plastic cups (750 mL volume), with 4 rice seeds sown in each cup. Routine management followed sowing. The soil in the plastic cups was a mixture of plant growth substrate, vermiculite, and perlite in a 4:3:3 (V / V / V) ratio. The plant height of the rice seedlings in the plastic cups was measured on days 3, 6, and 9. Twenty seedlings were randomly selected from each group.

[0047] The measurement results showed that after seed dressing treatment with different concentrations of α-linolenic acid, the height of rice seedlings of Nanjing 9108 fluctuated at different time points compared with the control, but the overall height of seedlings treated with the seed dressing was higher than that of the control, with some concentrations even being significantly higher than the control. Figure 1 After seeds of Shenliangyou No. 1 were treated with different concentrations of α-linolenic acid, the seedling height of the rice seedlings in the early treatment group was lower than that of the control group. However, as time went on, the seedling height of the treatment group gradually caught up with and exceeded that of the control group. Figure 2 ).

[0048] The above results indicate that rice seeds treated with different concentrations of α-linolenic acid did not have any significant adverse effects overall, and the plant height of rice was even significantly higher than the control at some treatment concentrations.

[0049] Example 3

[0050] Seed treatment involved mixing alpha-linolenic acid (ALA) with 20% chlorantraniliprole suspension. Three concentrations of ALA were set: 4 mg / L, 40 mg / L, and 400 mg / L; a fixed amount of 2.5 mg of 20% chlorantraniliprole suspension was used. Nanjing 9108 grape variety was selected for seed treatment. Each 1 g of dry seed was treated with a mixture of 1 mL of different concentrations of ALA solution (4-400 mg / L) and 2.5 mg of 20% chlorantraniliprole suspension. A water treatment was used as a control (CK). The seed treatment method was the same as in Example 1. After being placed in a cool, shaded place for 24 hours, the seeds were sown in petri dishes lined with absorbent paper to maintain moisture. Germination rate and average germination time were then statistically analyzed.

[0051] The results of the determination of germination rate and germination time of rice seeds showed that after the seeds of Nanjing 9108 were treated with a mixture of different concentrations of α-linolenic acid and chlorantraniliprole suspension, there was no significant difference in germination rate and germination time compared with the water control (CK) (Table 3).

[0052] Table 3. Seed germination rate and germination time after seed treatment with a mixture of α-linolenic acid and chlorantraniliprole suspension.

[0053]

[0054]

[0055] Note: Different lowercase letters indicate significant differences at the P<0.05 level.

[0056] Example 4

[0057] Seed treatment involved mixing alpha-linolenic acid (ALA) with 20% chlorantraniliprole suspension. Three concentrations of ALA were set: 4 mg / L, 40 mg / L, and 400 mg / L; a fixed amount of 2.5 mg of 20% chlorantraniliprole suspension was used. Nanjing 9108 rice variety was selected for seed treatment. Each 1 g of dry seed was treated with a mixture of 1 mL of different concentrations of ALA solution (4-400 mg / L) and 2.5 mg of 20% chlorantraniliprole suspension. A water treatment was used as a control (CK). The seed treatment method was the same as in Example 1. After treatment, the seeds were sown in disposable plastic cups (750 mL volume), with 4 rice seeds sown in each cup. Post-sowing management was standard. The soil in the plastic cups consisted of a mixture of plant growth substrate, vermiculite, and perlite in a 4:3:3 (V / V / V) ratio. The plant height of the rice seedlings in the plastic cups was measured on days 3, 6, and 9. Twenty plants were randomly measured in each group.

[0058] The measurement results showed that after seed treatment with a mixture of α-linolenic acid and chlorantraniliprole suspension at different concentrations, the height of rice seedlings of Nanjing 9108 fluctuated at different measurement time points compared with the control. However, the overall height of the seedlings treated with seed treatment was higher than that of the water control, and some concentrations were even significantly higher than the control. Figure 3 The above results indicate that seed dressing with different concentrations of α-linolenic acid and chlorantraniliprole suspension did not have a significant adverse effect on the overall growth of rice, and the plant height of rice was significantly higher than that of the control at certain treatment concentrations.

[0059] Example 5

[0060] Three concentrations of alpha-linolenic acid (ALA) were set: 4 mg / L, 40 mg / L, and 400 mg / L. Nanjing 9108 (japonica rice) and Shenliangyou 1 (indica rice) were used for seed treatment. Water treatment served as the control (CK). Each 1 g of dry seed was treated with 1 mL of different concentrations of ALA solution (4-400 mg / L). The seed treatment method was the same as in Example 1. After treatment, the seeds were sown in disposable plastic cups. Each plastic cup had a volume of 750 mL, and 4 rice seeds were sown in each cup. The soil in the plastic cups was a mixture of plant growth substrate and vermiculite in a 6:4 (V / V) ratio. On the 30th day after sowing, newly hatched rice stem borer larvae were inoculated into the rice seedlings in the plastic cups. Ten test larvae were inoculated into each cup, with 6 replicates per group. The number of live larvae was checked on the 6th day after inoculation, and the survival rate of the test larvae was statistically analyzed.

[0061] Data analysis shows that after rice seeds were treated with different concentrations of alpha-linolenic acid, the survival rate of rice stem borer gradually decreased with increasing alpha-linolenic acid concentration. Figure 4 The results of this experiment indicate that exogenous application of α-linolenic acid through seed dressing can significantly improve the resistance of rice to rice stem borer.

[0062] Example 6

[0063] Seed treatment involved mixing alpha-linolenic acid (ALA) with 20% chlorantraniliprole suspension. Three concentrations of ALA were set: 4 mg / L, 40 mg / L, and 400 mg / L; a fixed amount of 20% chlorantraniliprole suspension was used (2.5 mg). Additionally, a treatment using 20% ​​chlorantraniliprole suspension alone at a dosage of 5 mg was included (existing reports clearly indicate that the control effect of 20% chlorantraniliprole suspension significantly weakens below 5 mg / g of rice seed). Seeds of Nanjing 9108 (japonica rice) and Shenliangyou 1 (indica rice) were used for treatment. Each 1 g of dry seed was treated with a mixture of 1 mL of different concentrations of ALA solution (4-400 mg / L) and 2.5 mg of 20% chlorantraniliprole suspension, as well as a treatment using 5 mg of 20% chlorantraniliprole suspension alone. A water treatment served as a control (CK). After treatment, the seeds were sown in disposable plastic cups. The plastic cups had a volume of 750 mL, and each cup was used to sow 4 rice seeds. The soil in the plastic cups was a mixture of plant growth substrate and vermiculite in a 6:4 (V / V) ratio. The rice seedlings in the plastic cups were inoculated with newly hatched rice stem borer larvae at different stages after sowing. Ten test larvae were inoculated into each cup, with 10 replicates per group. The number of live larvae was checked 5-7 days after inoculation, and the survival rate of the test larvae was statistically analyzed.

[0064] Bioassay results on Nanjing 9108 rice plants showed that at days 22, 30, and 40, the survival rate of insects in each treatment group was below 10%, significantly different from the control (CK). Furthermore, there was no significant difference in insect survival rate between the synergistic seed treatment group (α-linolenic acid and chlorantraniliprole suspension) and the group treated with chlorantraniliprole suspension alone. At day 52, the insect survival rate in the synergistic seed treatment group increased slightly but remained significantly different from the control. Figure 5 A). Regarding seed treatment with 5 mg of 20% chlorantraniliprole suspension alone, in most cases, the lethal effect on rice stem borers was not significantly different when used in combination with α-linolenic acid and a reduced dose of chlorantraniliprole (2.5 mg of 20% chlorantraniliprole suspension). Figure 5 A).

[0065] Bioassay results on Shenliangyou No. 1 rice plants showed that, on days 23, 35, and 45 after seed dressing treatment, the survival rate of the control (CK) group was between 60-70%, while the survival rate of each treatment group was below 10%, showing extremely significant differences from the control group and no significant differences among the treatment groups. On day 62, the survival rate of the test insects in each treatment group increased, but it was still extremely significant compared with the control group, and there were no significant differences among the different treatment groups. Figure 5B). For seed treatment with 5 mg of 20% chlorantraniliprole suspension alone, there was no significant difference in lethality against rice stem borer at all test points (from 23 to 62 days) between treatments with α-linolenic acid and synergistic treatments with reduced doses of chlorantraniliprole (2.5 mg of 20% chlorantraniliprole suspension). Figure 5 B).

[0066] The above experimental results show that when using seed-mixing treatment technology to control pests on rice, the addition of exogenous α-linolenic acid can maintain a good control effect on pests while reducing the amount of chemical pesticides used.

[0067] Example 7

[0068] Seed treatment involved mixing alpha-linolenic acid (ALA) with 20% chlorantraniliprole suspension. Three concentrations of ALA were used: 4 mg / L, 40 mg / L, and 400 mg / L. A fixed amount of 20% chlorantraniliprole suspension was used: 2.5 mg. Additionally, a treatment using 5 mg of 20% chlorantraniliprole suspension alone was included. Corn (variety: Stada 221) seeds were used for seed treatment. Each 1 g of dry seed was treated with a mixture of 1 mL of different concentrations of ALA (4-400 mg / L) and 2.5 mg of 20% chlorantraniliprole suspension, as well as a treatment using 5 mg of 20% chlorantraniliprole suspension alone. A water treatment was used as a control (CK). The seed treatment method was the same as in Example 1. After being placed in a cool place for 24 hours, seeds were sown into disposable plastic cups (750 mL capacity), with 3 seeds sown in each cup. Post-sowing management was standard. The soil for the plastic cups was a mixture of plant growth substrate, vermiculite, and perlite in a 4:3:3 (V / V / V) ratio. The cabbage plants in the plastic cups had their height measured on days 3, 6, and 9. Twenty plants were randomly selected from each group.

[0069] Measurements of maize plant height after seed treatment showed that on day 3, there was no significant difference in plant height between the treatment groups and the water control (CK), and there were also no significant differences among the treatment groups. On day 6, the plant height of all treatment groups was higher than that of the CK group. The treatment group treated with a mixture of 40 mg / L alpha-linolenic acid and 2.5 mg of 20% chlorantraniliprole suspension (T2) showed a significant difference from the CK group, while the other treatment groups showed no significant difference from the CK group. On day 9, the treatment group treated with a mixture of 400 mg / L alpha-linolenic acid and 2.5 mg of 20% chlorantraniliprole suspension (T3) showed a significant difference from the T2 group, but there was no significant difference between any of the treatment groups and the CK group. Figure 6The results of this experiment show that, within a certain dosage range, seed dressing treatment with different concentrations of α-linolenic acid and 20% chlorantraniliprole suspension has no significant adverse effects on the overall growth of maize. In fact, at some treatment concentrations, the plant height of maize was even significantly higher than that of the control. These results are basically consistent with the test results in rice (Example 4).

[0070] Example 8

[0071] Seed treatment involved mixing alpha-linolenic acid (ALA) with 20% chlorantraniliprole suspension. Three concentrations of ALA were used: 4 mg / L, 40 mg / L, and 400 mg / L. A fixed dose of 2.5 mg of 20% chlorantraniliprole suspension was used. Additionally, a treatment using only 5 mg of 20% chlorantraniliprole suspension was included. Corn (variety: Stada 221) seeds were used for seed treatment. Each 1 g of dry seeds was treated with a mixture of 1 mL of different concentrations of ALA (4-400 mg / L) and 2.5 mg of 20% chlorantraniliprole suspension, as well as a treatment using only 5 mg of 20% chlorantraniliprole suspension. A water treatment was used as a control (CK). The seed treatment method was the same as in Example 1. After being placed in a cool, shaded place for 24 hours, seeds were sown into disposable plastic cups (750 mL capacity), with 3 seeds sown in each cup. Post-sowing management followed standard procedures. The soil for planting in plastic cups was a mixture of plant growth substrate and vermiculite in a 6:4 (V / V) ratio. Newly hatched fall armyworm larvae were inoculated 30 days after sowing. Ten larvae were inoculated per cup of rice seedling, with 10 replicates per group. The number of live larvae was checked 5 days after inoculation, and the survival rate was statistically analyzed.

[0072] Bioassay results showed that seed treatment significantly increased the lethality of corn against fall armyworm. Specifically, when the amount of chemical pesticide (chlorantraniliprole) was halved, synergistic seed treatments of α-linolenic acid and 20% chlorantraniliprole suspension (T1, T2, T3) also achieved good insecticidal effects, comparable to the insecticidal effect of seed treatment with a high dose of 20% chlorantraniliprole suspension alone (T4). Figure 7 The results of this experiment further demonstrate that when using seed dressing for pest control on corn, the exogenous addition of α-linolenic acid can maintain good pest control effects while reducing the amount of chemical pesticides used. These results are consistent with those obtained from tests on rice (Example 6).

[0073] Example 9

[0074] Seed treatment involved mixing alpha-linolenic acid (ALA) with 20% chlorantraniliprole suspension. Three concentrations of ALA were used: 4 mg / L, 40 mg / L, and 400 mg / L. A fixed amount of 20% chlorantraniliprole suspension was used. Additionally, a treatment using only 5 mg of 20% chlorantraniliprole suspension was included. Cabbage (variety: Jingfeng No. 1) seeds were used for seed treatment. Each 1 g of dry seeds was treated with a mixture of 1 mL of different concentrations of ALA (4-400 mg / L) and 2.5 mg of 20% chlorantraniliprole suspension, as well as a treatment using only 5 mg of 20% chlorantraniliprole suspension. A water treatment was used as a control (CK). The seed treatment method was the same as in Example 1. After being placed in a cool place for 24 hours, seeds were sown into disposable plastic cups (750 mL capacity), with 3 seeds sown in each cup. Post-sowing management followed standard procedures. The soil for the plastic cups was a mixture of plant growth substrate, vermiculite, and perlite in a 4:3:3 (V / V / V) ratio. The cabbage plants in the plastic cups had their height measured on days 3, 6, and 9. Twenty plants were randomly selected from each group.

[0075] The measurement results showed that on day 3, the plant height of treatment groups T2 and T4 was significantly smaller than that of the control group (CK), while the plant height of the other two treatment groups was not significantly different from that of the control group; on day 6, there was no significant difference in plant height between the treatment groups and the control group, and there was also no significant difference between the treatment groups; on day 9, the plant height of treatment group T3 was significantly higher than that of the control group, while the plant height of the other treatment groups was not significantly different from that of the control group. Figure 8 The results of this experiment indicate that seed treatment with pesticides had some adverse effects on the growth of cabbage in the early stages, but these adverse effects gradually disappeared over time, and the seed treatment also had a certain promoting effect on the growth of cabbage.

[0076] Example 10

[0077] Seed treatment involved mixing alpha-linolenic acid (ALA) with 20% chlorantraniliprole suspension. Three concentrations of ALA were used: 4 mg / L, 40 mg / L, and 400 mg / L. A fixed amount of 20% chlorantraniliprole suspension was used. Additionally, a treatment using only 5 mg of 20% chlorantraniliprole suspension was included. Cabbage (variety: Jingfeng No. 1) seeds were used for seed treatment. Each 1 g of dry seeds was treated with a mixture of 1 mL of different concentrations of ALA (4-400 mg / L) and 2.5 mg of 20% chlorantraniliprole suspension, as well as a treatment using only 5 mg of 20% chlorantraniliprole suspension. A water treatment was used as a control (CK). The seed treatment method was the same as in Example 1. After being placed in a cool place for 24 hours, seeds were sown into disposable plastic cups (750 mL capacity), with 3 seeds sown in each cup. Post-sowing management followed standard procedures. The soil for planting in plastic cups was a mixture of plant growth substrate and vermiculite in a 6:4 (V / V) ratio. Newly hatched diamondback moth larvae were inoculated 30 days after sowing. Ten test larvae were inoculated per cup of rice seedling, with 10 replicates per group. The number of live larvae was checked 5 days after inoculation, and the survival rate was statistically analyzed.

[0078] Bioassay results for the diamondback moth showed that seed treatment with alpha-linolenic acid alone could enhance the resistance of cabbage to the diamondback moth; exogenous addition of alpha-linolenic acid could maintain a good lethal effect on the diamondback moth while reducing the amount of chemical pesticides used. Figure 9 This result is consistent with the test results on rice (Example 6) and corn (Example 8).

[0079] In summary, this invention proposes a biological combination agent for controlling crop pests. Seed treatment using this biological combination agent has no significant adverse effects on the germination and growth of crops (rice, corn, cabbage), and even shows a significant promoting effect at certain treatment concentrations. Furthermore, it still exhibits good lethality against relevant pests even with significant reductions in the use of chemical pesticides. This invention provides important guidance for the development of greener and more efficient crop seed treatment technologies for pest control.

Claims

1. A biological combination agent for controlling crop pests, characterized in that, The biological combination formulation includes the plant resistance inducer α-linolenic acid and the pesticide chlorantraniliprole, with a mass ratio of 0.004-0.4:0.5-5.

2. The biological combination agent for controlling crop pests according to claim 1, characterized in that, The biological combination formulation includes the plant resistance inducer α-linolenic acid and 20% chlorantraniliprole suspension, with a mass ratio of 0.004-0.4:2.5-5.

3. The biological combination agent for controlling crop pests according to claim 1, characterized in that, The crop mentioned is rice, corn, or cabbage.

4. The biological combination agent for controlling crop pests according to claim 1, characterized in that, The pests mentioned are rice stem borer, rice leaf roller, fall armyworm, corn borer, cotton bollworm, armyworm, diamondback moth, or cabbage caterpillar.

5. The application of the biological combination preparation for controlling crop pests as described in claim 1 in the control of crop pests.

6. The application according to claim 5, characterized in that, The biological combination preparations used to control crop pests are applied to the control of crop pests through seed dressing.

7. The application according to claim 5, characterized in that, The specific process of the application is as follows: seeds are treated with a mixture of plant inducer α-linolenic acid and chlorantraniliprole, and then sown after thorough mixing.

8. The application according to claim 7, characterized in that, For every 1 g of dry seeds, mix thoroughly with 1 mL of α-linolenic acid solution at a concentration of 4-400 mg / L and 2.5-5 mg of 20% chlorantraniliprole suspension.

9. The application according to claim 8, characterized in that, After thoroughly mixing, the seed dressing process is complete. Place the seeds in a cool, shaded place for 12-24 hours before sowing.

10. The application according to claim 7, characterized in that, The sowing refers to the direct sowing of seeds into the field by direct sowing, or the raising of seedlings in seedling trays followed by transplanting.

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