Feed additive for improving superovulation effect of cows and preparation method thereof

Abstract

The invention provides a feed additive for improving the superovulation effect of cows and a preparation method of the feed additive, and the feed additive comprises the following components in parts by mass: 4-6 parts of astragaloside, 4-6 parts of apigenin, 4-6 parts of kaempferol, 0.4-0.6 part of luteolin, 0.4-0.6 part of isoquercitrin, 0.2-0.4 part of formononetin and 0.2-0.4 part of quercetin. By using the feed additive, the ovulation number and the anti-oxidation and anti-inflammatory capacity of cows can be increased, meanwhile, the natural active ingredients in the feed additive do not need complex chemical synthesis steps, the breeding cost is reduced, animal health is improved, green and safe breeding is guaranteed, and a basis is provided for green development of modern animal husbandry.

Description

Technical Field

[0001] The present invention relates to the technical field of feed additives, and in particular to a feed additive for improving the superovulation effect of cows and a preparation method thereof. Background Art

[0002] Low reproductive efficiency in cows has become a major constraint on improving the quality and efficiency of the modern beef cattle industry. Unstable superovulation results, oxidative stress, and inflammatory responses are key factors affecting embryo transfer success and cow health. Current superovulation techniques rely primarily on exogenous hormones to regulate follicular development. This method is subject to significant individual variability, and long-term or excessive use can easily lead to reproductive disorders such as ovarian cysts and ovulation disorders. Furthermore, drug residues may be transmitted through the food chain, which is inconsistent with the trend of sustainable farming.

[0003] Furthermore, oxidative stress and chronic inflammation are common pathological conditions in cows during the peripartum period. Excessive free radical accumulation can damage oocytes, interfering with follicular development and embryo implantation, while persistent inflammation can disrupt the estrous cycle by inhibiting gonadotropin-releasing hormone secretion.

[0004] Flavonoids are a class of plant secondary metabolites with a basic C6-C3-C6 skeleton. Based on their structural differences, they are generally divided into subclasses such as flavonoids, flavonols, flavanols, flavanones, isoflavones, chalcones, and anthocyanins. While preliminary research has examined the effects of flavonoids on the reproductive and growth performance of monogastric animals, no studies have yet demonstrated their effects on the reproductive performance of ruminants. Therefore, the development of novel feed additives with combined superovulation enhancement, antioxidant, and anti-inflammatory properties is crucial for overcoming existing technological bottlenecks and achieving green development in the beef cattle industry.

[0005] In view of this, the present invention is proposed. Summary of the Invention

[0006] The present invention provides a feed additive for improving the superovulation effect of cows and a preparation method. The present invention is mainly intended to solve the problems in the prior art such as low reproductive efficiency of cows, low embryo transplantation success rate due to unstable ovulation, oxidative stress and inflammatory response, and the inability to ensure the health of cows, which in turn leads to a significant reduction in the reproductive capacity of cows and restricts the development of the modern beef cattle industry.

[0007] In order to solve the above problems, the present invention adopts the following technical solutions:

[0008] The present invention provides a feed additive for improving the superovulation effect of cows, characterized by comprising the following components, calculated in parts by mass:

[0009] 4-6 parts astragaloside, 4-6 parts apigenin, 4-6 parts kaempferol, 0.4-0.6 parts luteolin, 0.4-0.6 parts isoquercetin, 0.2-0.4 parts formononetin and 0.2-0.4 parts quercetin.

[0010] In the present invention, astragaloside, apigenin, kaempferol, luteolin, isoquercetin, formononetin and quercetin are used to prepare feed additives. Astragaloside, apigenin, kaempferol, luteolin, isoquercetin, formononetin and quercetin are all flavonoids or their derivatives, and all have a typical core skeleton of flavonoids. Due to different substitution groups, ring connection modes or glycosylation modifications, diverse structures are formed, thereby exhibiting different biological activities such as antioxidant, anti-inflammatory, and metabolic regulation.

[0011] Astragaloside is an active ingredient extracted from Astragalus root, primarily comprising saponins and flavonoids. It regulates follicular development, regulates secretion balance, and improves ovarian function. It can enhance follicular quality and ovulation by promoting the secretion of follicular growth hormone or directly stimulating follicular development. It also has antioxidant properties, reducing oxidative stress damage to follicles and indirectly protecting germ cell function. It can also regulate estrogen levels, improve reproductive cycle abnormalities, create favorable conditions for follicle maturation and ovulation, enhance energy metabolism in ovarian tissue, and improve ovarian microcirculation, thereby supporting follicular development and ovulation.

[0012] The apigenin used is a flavonoid compound that can scavenge free radicals, relieve oxidative stress, and protect cells from damage. It can also inhibit inflammatory factors and has antioxidant and anti-inflammatory functions. At the same time, apigenin, as an antioxidant ingredient, can also indirectly promote follicle development by improving the endocrine environment. Combined with astragaloside, it can greatly improve the ovulation quality of cows.

[0013] Kaempferol is also a flavonoid compound, which also has antioxidant and anti-inflammatory functions. Since apigenin and kaempferol are both flavonoid compounds, they can scavenge free radicals and regulate inflammatory pathways through different pathways. Apigenin can regulate receptors and relieve inflammation, while kaempferol inhibits metabolism-related inflammation. Their combined use forms a complementary effect, which can greatly enhance the overall antioxidant and anti-inflammatory effects.

[0014] Luteolin is also a flavonoid compound, which also has antioxidant and anti-inflammatory effects. It can be used as a natural antioxidant in combination with apigenin to scavenge free radicals, reduce the damage of oxidative stress to intestinal epithelial cells, regulate the intestinal metabolism of cows, and reduce the incidence of cows. It can regulate the rumen microbial composition of cows, inhibit the metabolic activity of methanogens, and reduce methane production. Its antibacterial properties can inhibit microbial degradation of cellulose and reduce the activity of key enzymes in methane synthesis. At the same time, cows can reduce their dependence on antibiotics after consumption, maintain the balance of rumen flora by inhibiting pathogenic bacteria, promote mitochondrial autophagy, repair tight junction proteins in the cow intestine, and reduce intestinal damage. Luteolin has inhibitory effects on both Gram-negative bacteria (such as Escherichia coli) and Gram-positive bacteria (such as Staphylococcus aureus). Combined with astragaloside, it can produce a synergistic antibacterial effect, reduce the risk of drug-resistant bacteria, and further ensure the health of cows.

[0015] The present invention also uses isoquercetin and quercetin, wherein quercetin belongs to the flavonol compound, is poorly soluble in water, and is passively diffused and absorbed, while isoquercetin is a glucoside derivative of quercetin, belongs to the flavonoid glycosides, has good water solubility, higher bioavailability, and faster absorption. The two are used in synergistic use. Quercetin, as an aglycone form, needs to rely on intestinal flora to hydrolyze the glycoside before it can be absorbed, and has low bioavailability, while isoquercetin is in the glycoside form, and its water solubility and intestinal absorption efficiency are significantly improved, which can be used in the present invention. It is quickly converted into quercetin by β-glucosidase, improving the overall utilization rate. The combination of the two can increase the absorption efficiency to more than forty times that of single quercetin, shortening the onset time. At the same time, quercetin directly eliminates free radicals, while isoquercetin activates the Nrf2 pathway to enhance the activity of endogenous antioxidant enzymes and exert antioxidant effects. Quercetin inhibits inflammatory factors, and isoquercetin blocks the NF-kB signaling pathway, covering a wider inflammatory regulatory network, which can greatly enhance the antioxidant and anti-inflammatory capabilities of cows.

[0016] Finally, the present invention also uses formononetin, which also belongs to the flavonoids. It scavenges free radicals, enhances the activity of antioxidant enzymes, alleviates oxidative stress damage, inhibits inflammatory signal pathways, and reduces the release of inflammatory factors, thereby achieving good antioxidant and anti-inflammatory effects. At the same time, it can also bind to estrogen receptors, improve bone quality, and has a weak stimulating effect on the mammary gland and uterus, with high safety. While improving the antioxidant and anti-inflammatory capabilities of cows, it will not affect the ovulation function of cows.

[0017] Preferably, the feed additive includes the following components, calculated by weight:

[0018] 4.5-5.5 parts astragaloside, 4.5-5.5 parts apigenin, 4.5-5.5 parts kaempferol, 0.45-0.55 parts luteolin, 0.45-0.55 parts isoquercetin, 0.25-0.35 parts formononetin and 0.25-0.35 parts quercetin.

[0019] Preferably, the feed additive includes the following components, calculated by weight:

[0020] 5 parts astragaloside, 5 parts apigenin, 5 parts kaempferol, 0.5 parts luteolin, 0.5 parts isoquercetin, 0.3 parts formononetin and 0.3 parts quercetin.

[0021] The present invention also provides a method for preparing the above-mentioned feed additive for improving the superovulation effect of cows, which comprises the following steps:

[0022] The powders of astragaloside, apigenin, kaempferol, luteolin, isoquercetin, formononetin and quercetin were respectively passed through a 60-mesh sieve;

[0023] Astragaloside, apigenin, kaempferol, luteolin, isoquercetin, formononetin, and quercetin were weighed;

[0024] and mixing to obtain a feed additive.

[0025] Preferably, the mixing comprises the steps of:

[0026] Astragaloside, apigenin, kaempferol, luteolin, isoquercetin, formononetin and quercetin were mixed by the equal-increment method: 1 / 2 of the powder of any two components was mixed for 2 minutes, and then the remaining 1 / 2 was added and the mixture was repeated until uniform;

[0027] Continue adding the third and fourth ingredients to the mixture according to the above method until the powders of all the components remain. The amount added each time should not exceed 1 times the current total amount. Ensure that there is no visible difference after each step of mixing.

[0028] Preferably, the mixing speed is 20-30 rpm.

[0029] Preferably, the method further comprises the following steps after obtaining the feed additive:

[0030] Store feed additives in a sealed container away from light.

[0031] The present invention also provides a cow feed, which comprises the above-mentioned feed additive and / or the feed additive prepared by the above-mentioned preparation method, and a complete mixed diet.

[0032] Preferably, the mass ratio of the feed additive to the complete mixed feed is 1:(700-800).

[0033] Preferably, the present invention also provides a method for preparing the above-mentioned cow feed, which comprises the following steps:

[0034] Stir the feed additive and premix until they are initially mixed evenly;

[0035] Add the concentrate and stir until it is evenly mixed;

[0036] Add the complete mixed ration and continue stirring for 5-10 minutes until it is evenly mixed to obtain cow feed.

[0037] The present invention uses the above components to prepare cow feed, which can greatly improve the ovulation number, antioxidant and anti-inflammatory capabilities of cows, and provides a huge guarantee for the development of my country's beef cattle industry.

[0038] Compared with the prior art, the present invention has at least the following advantages:

[0039] 1. The present invention is the first to combine astragaloside, apigenin, kaempferol, luteolin, isoquercetin, formononetin and quercetin to prepare a feed additive, which is applied to the superovulation effect, can effectively improve the superovulation effect of cows, and can be promoted and applied in farms.

[0040] 2. The feed additive proposed by the present invention can effectively improve the number and quality of cow embryos, reduce oxidative damage, and lower the level of inflammatory factors.

[0041] 3. This invention offers significant advantages over traditional antibiotics, hormones, and other chemical substances. Flavonoid raw materials are low-cost and the extraction process is simple, eliminating the need for complex chemical synthesis steps, effectively reducing breeding costs. Compared to traditional antibiotics, this invention utilizes natural active ingredients to regulate reproductive performance, improve animal health, and ensure green and safe breeding, providing a foundation for the green development of modern animal husbandry.

[0042] 4. The preparation method of the feed additive provided by the present invention has simple steps and is easy to operate. After training, the user can quickly get started and realize industrial production.

[0043] 5. The implementation method of the present invention is simple to operate. It only requires adding the feed additive directly to the complete mixed diet according to the additive dosage, without relying on complex equipment or professional technical support.

[0044] 6. The feed additive provided by this invention can effectively increase the number and quality of embryos in cows, reduce oxidative damage, and lower the levels of inflammatory factors. Flavonoids offer significant cost advantages and ease of use compared to traditional reproductive hormones. They can mitigate reproductive system risks such as ovarian abnormalities caused by long-term hormone intervention while maintaining the green and healthy nature of the breeding process, providing technical support for the sustainable development of the industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Various other advantages and benefits will become apparent to those skilled in the art upon reading the detailed description of the preferred embodiment below. The accompanying drawings are for illustration purposes only and are not to be considered as limiting the present invention. The same reference symbols are used throughout the drawings to represent the same components. In the drawings:

[0046] Figure 1 A schematic diagram of the feed additive configuration provided by an embodiment of the present invention;

[0047] Figure 2 A schematic diagram of a feed additive provided by an embodiment of the present invention;

[0048] Figure 3 Schematic diagram of adding the feed additive provided by an embodiment of the present invention to a total mixed ration;

[0049] Figure 4 Schematic diagram of feeding and testing of feed additives provided by the embodiment of the present invention Figure 1 ;

[0050] Figure 5 Schematic diagram of feeding and testing of feed additives provided by the embodiment of the present invention Figure 2 . DETAILED DESCRIPTION

[0051] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention more clearly understood, the embodiments of the present invention are further described in detail below in conjunction with the embodiments and drawings. Here, the illustrative embodiments of the present invention and their descriptions are used to explain the present invention, but are not intended to limit the present invention.

[0052] It should be understood that the terms "comprises / comprising," "consisting of," or any other variations thereof are intended to encompass non-exclusive inclusion, such that a product, apparatus, process, or method that includes a list of elements includes not only those elements but also, if necessary, other elements not explicitly listed, or elements inherent to such product, apparatus, process, or method. In the absence of further limitations, elements defined by the phrases "comprises / comprising," "consisting of," do not preclude the presence of additional identical elements in the product, apparatus, process, or method that includes the elements.

[0053] Finally, if the specific conditions in the examples are not specified, the experiments were carried out according to conventional conditions or those recommended by the manufacturer. The reagents or instruments used, if the manufacturer is not specified, are all commercially available conventional products.

[0054] Example 1

[0055] This embodiment provides a cow feed, which mainly includes a complete mixed diet and feed additives, and is mainly intended to solve the problems of unstable ovulation of cows in the prior art and the easy occurrence of oxidative stress and inflammation.

[0056] This embodiment also provides a method for preparing the above-mentioned cow feed, which comprises the following steps:

[0057] 1. Preparation of feed additives

[0058] a. Sieve astragaloside, apigenin, kaempferol, luteolin, isoquercetin, formononetin, and quercetin through a 60-mesh sieve to remove lumps or large particles to ensure consistent particle size and reduce stratification caused by particle size differences;

[0059] b. Weigh 5 parts of astragaloside, 5 parts of apigenin, 5 parts of kaempferol, 0.5 parts of luteolin, 0.5 parts of isoquercetin, 0.3 parts of formononetin and 0.3 parts of quercetin

[0060] c. The weighed powders of astragaloside, apigenin, kaempferol, luteolin, isoquercetin, formononetin and quercetin were mixed in equal parts:

[0061] First, spray the inner wall of the small mixer with 75% ethanol to perform antistatic treatment. No residue will remain after the ethanol evaporates.

[0062] Then take out 1 / 2 of each of astragaloside and apigenin and put them into a small mixer, mix at a speed of 20-30 rpm for 2 minutes, then add the remaining amount, and repeat mixing until uniform;

[0063] Then, the mixed astragaloside and apigenin are mixed with the remaining components in sequence according to the above method, and the amount added each time does not exceed twice the current total amount, ensuring that there is no visible difference after each mixing step;

[0064] d. After mixing, the feed additive is obtained and stored in a sealed container away from light. Figure 2 As shown, the feed additive is in the form of light yellow powder.

[0065] 2. Prepare cow feed

[0066] Stir the feed additive and premix until they are preliminarily mixed;

[0067] Add the concentrate and stir until it is evenly mixed;

[0068] Weigh the total mixed ration at a ratio of 750:1, put it into the TMR mixer, and stir for 5-10 minutes until it is evenly mixed to obtain the cow feed. Figure 3 As shown, Figure 2Add the feed additives shown in the table to the complete mixed ration and mix well before feeding.

[0069] In this embodiment, in order to ensure the use effect of the cow feed, the mixing speed in step 1 is selected to be 15 revolutions per minute, and the stirring time in step 2 is selected to be 5-10 minutes.

[0070] Of course, in actual operation, the stirring speed can be adjusted according to actual conditions to ensure the mixing effect.

[0071] In addition, when using a small blender, it is necessary to ensure that the powder loading does not exceed 50% of the volume.

[0072] It should be noted that in this embodiment, all components are expressed in parts by mass and in mass ratios.

[0073] Examples 2-6

[0074] The specific implementation is consistent with that of Example 1, except for the differences shown in Table 1 below:

[0075] Table 1 Feed additive ingredients

[0076]

[0077] Experimental Example 1

[0078] In this experiment, 60 donor cows (cows) were randomly divided into 6 experimental groups, each of which contained 10 donor cows. The cows were fed the cow feeds prepared in Examples 1-6 in the order of experimental groups 1-6. Figure 4 After 40 days of feeding, the donor cows were superovulated and serum was collected. Figure 5 As shown, the total number of flushed embryos, unfertilized embryos, degenerated embryos, and available embryos of the donor cattle were observed and recorded, as shown in Table 2; the number of grade A embryos and grade B embryos of the donor cattle were observed and recorded, as shown in Table 3; the number of blastocysts, early blastocysts, and morulas of the donor cattle were observed and recorded, as shown in Table 4. At the same time, the antioxidant capacity indicators (tested by the kit) catalase (CAT), superoxide dismutase (SOD) activity, malondialdehyde (MDA), reactive oxygen species (ROS), glutathione peroxidase (GSH-Px) content, and total antioxidant capacity (T-AOC) were detected, as shown in Table 5; the inflammatory factor indicators (tested by the kit) interleukin 6 (IL-6), interleukin 10 (IL-10), and tumor necrosis factor-α (TNF-α) content were detected, as shown in Table 6.

[0079] Table 2 Effects of feed additives on the number of donor bovine embryos

[0080]

[0081] Table 3 Effects of feed additives on the quality of donor bovine embryos

[0082]

[0083] Table 4 Effects of feed additives on the developmental stages of donor bovine embryos

[0084]

[0085]

[0086] Table 5 Effects of feed additives on antioxidant activity of donor bovine serum

[0087]

[0088] Table 6 Effects of feed additives on immunity of donor cattle

[0089] Donor cattle feed source IL-6 / (ng / L) IL-10 / (ng / L) TNF-α / (ng / L) Experimental Group 1 Example 1 <![CDATA[8.61 e ]]> <![CDATA[41.68 a ]]> <![CDATA[131.30 e ]]> Experimental Group 2 Example 2 <![CDATA[12.30 bc ]]> <![CDATA[35.20 b ]]> <![CDATA[158.40 b ]]> Experimental Group 3 Example 3 <![CDATA[15.20 a ]]> <![CDATA[28.50 c ]]> <![CDATA[180.20 a ]]> Experimental Group 4 Example 4 <![CDATA[10.50 d ]]> <![CDATA[38.60 ab ]]> <![CDATA[142.50 d ]]> Experimental Group 5 Example 5 <![CDATA[11.80 c ]]> <![CDATA[36.80 ab ]]> <![CDATA[150.60 c ]]> Experimental Group 6 Example 6 <![CDATA[13.50 b ]]> <![CDATA[32.40 b ]]> <![CDATA[165.80 b ]]>

[0090] It can be seen from the data in Tables 2 to 6 above that experimental group 1 has the best effect, and exhibits the best superovulation, antioxidant and anti-inflammatory effects due to the synergistic effect of the ingredients. The feed additive of the present invention has the best effect when using 5 parts of astragaloside, 5 parts of apigenin, 5 parts of kaempferol, 0.5 parts of luteolin, 0.5 parts of isoquercetin, 0.3 parts of formononetin and 0.3 parts of quercetin.

[0091] Comparative Example 1

[0092] The specific implementation method is the same as that of Example 1, the only difference being that no feed additive is used in the feed additive.

[0093] Comparative Example 2

[0094] The specific implementation method is the same as that of Example 1, the only difference being that astragaloside is not added to the feed additive.

[0095] Comparative Example 3

[0096] The specific implementation method is consistent with Example 1, the only difference being that apigenin is not added to the feed additive.

[0097] Comparative Example 4

[0098] The specific implementation method is consistent with Example 1, the only difference being that kaempferol is not added to the feed additive.

[0099] Comparative Example 5

[0100] The specific implementation method is consistent with Example 1, the only difference being that the mass ratio of feed additive to complete mixed diet is 1:400.

[0101] Comparative Example 6

[0102] The specific implementation method is consistent with Example 1, the only difference being that the mass ratio of the feed additive to the complete mixed diet is 1:1000.

[0103] Experimental Example 2

[0104] In this experiment, 60 donor cows (cows) were randomly divided into 6 experimental groups, each containing 10 donor cows. The cows were fed the cow feed prepared in Comparative Examples 1-6 in the order of experimental groups 7-12, and compared with experimental group 1. After 40 days of feeding, the donor cows were superovulated and serum was collected. The total number of embryos, unfertilized embryos, degenerated embryos, and usable embryos of the donor cows were observed and recorded, as shown in Table 7; the number of grade A embryos and grade B embryos of the donor cows were observed and recorded, as shown in Table 8; the number of blastocysts, early blastocysts, and morulas of the donor cows were observed and recorded. The number of mulberry embryos is shown in Table 9. At the same time, the antioxidant capacity indicators (tested by the kit) catalase (CAT), superoxide dismutase (SOD) activity, malondialdehyde (MDA), reactive oxygen species (ROS), glutathione peroxidase (GSH-Px) content, and total antioxidant capacity (T-AOC) are tested, as shown in Table 10; the inflammatory factor indicators (tested by the kit) interleukin 6 (IL-6), interleukin 10 (IL-10) and tumor necrosis factor-α (TNF-α) content are tested, as shown in Table 11.

[0105] Table 7 Comparison of the effects of feed additives (ingredients) on the number of donor bovine embryos

[0106]

[0107] Table 8 Comparison of the effects of feed additives (ingredients) on the quality of donor bovine embryos

[0108]

[0109] Table 9 Comparison of the effects of feed additives (ingredients) on the developmental stages of donor bovine embryos

[0110]

[0111] Table 10 Comparison of the effects of feed additives (ingredients) on the antioxidant activity of donor bovine serum

[0112]

[0113] Table 11 Comparison of the effects of feed additives (ingredients) on the immunity of donor cattle

[0114]

[0115]

[0116] The data in Tables 7 to 11 above show that experimental groups 7 to 10 have poor effects due to the lack of key ingredients (all ingredients are missing in experimental group 7, astragaloside is absent in experimental group 8, apigenin is absent in experimental group 9, and kaempferol is absent in experimental group 10). In particular, although the antioxidant capacity of experimental groups 8, 9, and 10 is partially maintained, it cannot compensate for the increased follicular atresia rate caused by the lack of astragaloside, apigenin, and kaempferol, further verifying the irreplaceable nature of the formula of the present invention.

[0117] Although the formula ratios of experimental groups 11 and 12 were consistent with those of experimental group 1, the bioavailability decreased significantly due to the deviation of the mixing ratio from the threshold of 1: (700-800). In experimental group 11, due to the excessively high concentration of additives (1:400), some flavonoid components aggregated and precipitated in TMR, resulting in the loss of active ingredients. At the same time, the high concentration induced mild cellular stress. In experimental group 12, due to the excessive dilution of the mixing ratio (1:1000), the active ingredients were adsorbed by TMR fibers, the antioxidant capacity was further weakened, and pro-inflammatory factors increased. The unbalanced mixing ratios of the two groups resulted in their effects being weaker than those of experimental groups 1-6, verifying the key role of the mixing ratio of 1:750 in maintaining the stability of flavonoids and the dose effect.

[0118] The cow feed provided by the present invention has at least the following advantages:

[0119] 1. The present invention is the first to apply the combination of astragaloside, apigenin, kaempferol, luteolin, isoquercetin, formononetin and quercetin in the field of superovulation, which can significantly improve the reproductive capacity of cows and can be promoted and applied in farms.

[0120] 2. The feed additive provided by the present invention can increase the total number of embryos produced, the number of usable embryos, the number of grade A embryos, and the morula rate of cows. It also improves the health of cows and significantly enhances their antioxidant and anti-inflammatory abilities.

[0121] 3. This invention offers significant advantages over traditional antibiotics, hormones, and other chemical substances. Flavonoid raw materials are low-cost and the extraction process is simple, eliminating the need for complex chemical synthesis steps, effectively reducing breeding costs. Compared to traditional antibiotics, this invention utilizes natural active ingredients to regulate reproductive performance, improve animal health, and ensure green and safe breeding, providing a foundation for the green development of modern animal husbandry.

[0122] 4. The implementation method of the present invention is simple to operate. Functional regulation can be achieved by directly adding the feed additives according to the added amount to the complete mixed diet without relying on complex equipment or professional technical support.

[0123] Finally, it should be understood that the above embodiments are merely exemplary embodiments for illustrating the principles of the present invention, and the present invention is not limited thereto. Those skilled in the art will be able to make various modifications and improvements without departing from the principles and essence of the present invention, and such modifications and improvements are also considered to be within the scope of protection of the present invention.

Claims

1. A feed additive for improving the superovulation effect of cows, characterized in that: It includes the following components, calculated by mass: 4-6 parts astragaloside, 4-6 parts apigenin, 4-6 parts kaempferol, 0.4-0.6 parts luteolin, 0.4-0.6 parts isoquercetin, 0.2-0.4 parts formononetin and 0.2-0.4 parts quercetin.

2. The feed additive according to claim 1, characterized in that It includes the following components, calculated by mass: 4.5-5.5 parts astragaloside, 4.5-5.5 parts apigenin, 4.5-5.5 parts kaempferol, 0.45-0.55 parts luteolin, 0.45-0.55 parts isoquercetin, 0.25-0.35 parts formononetin and 0.25-0.35 parts quercetin.

3. The feed additive according to claim 1, characterized in that It includes the following components, calculated by mass: 5 parts astragaloside, 5 parts apigenin, 5 parts kaempferol, 0.5 parts luteolin, 0.5 parts isoquercetin, 0.3 parts formononetin and 0.3 parts quercetin.

4. A method for preparing a feed additive for improving superovulation effect in cows according to any one of claims 1 to 3, characterized in that: The steps include: The powders of astragaloside, apigenin, kaempferol, luteolin, isoquercetin, formononetin and quercetin were respectively passed through a 60-mesh sieve; Astragaloside, apigenin, kaempferol, luteolin, isoquercetin, formononetin, and quercetin were weighed; and mixing to obtain a feed additive.

5. The preparation method according to claim 4, characterized in that The mixing comprises the steps of: Astragaloside, apigenin, kaempferol, luteolin, isoquercetin, formononetin and quercetin were mixed by the equal-increment method: 1 / 2 of the powder of any two components was mixed for 2 minutes, and then the remaining 1 / 2 was added and the mixture was repeated until uniform; Continue adding the third and fourth ingredients to the mixture according to the above method until the powders of all the components remain. The amount added each time should not exceed 1 times the current total amount. Ensure that there is no visible difference after each step of mixing.

6. The preparation method according to claim 5, characterized in that The mixing speed is 20-30 rpm.

7. The preparation method according to claim 4, characterized in that After obtaining the feed additive, the following steps are also included: Store feed additives in a sealed container away from light.

8. A cow feed, characterized in that: The invention comprises the feed additive according to any one of claims 1 to 3 and / or the feed additive prepared by the preparation method according to any one of claims 2 to 7, and a complete mixed diet.

9. The cow feed according to claim 8, characterized in that The mass ratio of the feed additive to the complete mixed feed is 1:(700-800).

10. The method for preparing the cow feed according to any one of claims 8 to 9, characterized in that: The steps include: Mix the feed additive with the premix, then add the concentrate and mix well, and finally add the complete mixed ration and stir for 5-10 minutes until the mixture is evenly mixed to obtain the cow feed.

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