Application of saccharomyces cerevisiae strain in preparation of crop early-stage growth promoter

Abstract

The invention discloses an application of a vichenille yeast strain in preparation of a crop early-stage growth promoter, and belongs to the technical field of microbial fertilizers. The vignich yeast strain is dissolved in purified water and mixed uniformly to be applied directly, and after the strain preparation is applied, the indoor experiment results prove that the yeast can remarkably promote the fresh weight growth of stems and leaves of wheat, lettuce and white radish, and particularly, the effect is optimal at the concentration of 1: 2000. In field experiments, the saccharomycetes agent has stable effects on wheat yield increase (12.6%), lettuce fresh weight increase (35%) and white radish root development (23% fresh weight increase), and can indirectly enhance crop yield and quality by improving photosynthetic efficiency (lettuce case) and stress resistance (wheat case).

Description

Technical Field

[0001] The invention belongs to the technical field of microbial fertilizers, and particularly relates to an application of a Vishnyk yeast strain in the preparation of an early growth promoter for crops. Background Art

[0002] In agricultural production, fertilizer plays a vital role in improving food yield and quality. To meet food demand, traditional agriculture relies heavily on chemical fertilizers and pesticides. However, long-term excessive use of chemical fertilizers and pesticides not only leads to soil degradation and environmental pollution, but also raises food safety concerns. Soil compaction, acidification, and a decrease in organic matter are becoming increasingly serious problems.

[0003] As a new type of biofertilizer, bacterial fertilizer has gradually become a research hotspot in the agricultural field due to its environmentally friendly, efficient, and sustainable characteristics. Bacterial fertilizer, with beneficial microorganisms as its core ingredients, improves the soil environment and promotes early crop growth through the microbial activities. It can convert insoluble nutrients in the soil into forms that can be absorbed by crops through nitrogen fixation, phosphorus solubilization, and potassium solubilization. It also secretes plant growth hormones, enhancing crop resistance to stress. The use of bacterial fertilizer not only reduces the use of chemical fertilizers but also improves soil structure and fertility, making it an agricultural input that aligns with the concepts of green agriculture and sustainable development.

[0004] In the context of green agricultural transformation, the industrialization of microbial fertilizers, as environmentally friendly biological agents, has significantly accelerated. However, their development still faces technical challenges such as insufficient functional diversity of strains and low product standardization. Summary of the Invention

[0005] The present invention provides a method for preparing an early crop growth promoter using a yeast strain of Vishnyk. This yeast strain can be dissolved in pure water and mixed thoroughly for direct application. Laboratory experiments have shown that the yeast significantly promotes the fresh weight growth of stems and leaves of wheat, lettuce, and white radish, with the most pronounced effect at a concentration of 1:2000. In field experiments, the yeast agent demonstrated a consistent effect on increasing wheat yield (12.6%), lettuce fresh weight (35%), and white radish root development (23% fresh weight increase). Furthermore, the yeast agent indirectly enhances crop yield and quality by improving photosynthetic efficiency (in the case of lettuce) and stress resistance (in the case of wheat).

[0006] The present invention is achieved through the following technical solutions:

[0007] A use of a Vishnyk yeast strain in preparing an early crop growth promoter, wherein the early crop growth promoter is prepared by dissolving the cultured Vishnyk yeast strain in pure water and mixing the mixture;

[0008] The mass concentration of the mixture of the Vishnyk yeast strain and purified water is 1:500 to 4000.

[0009] Preferably, the mass concentration of the mixture of the Vishnyk yeast strain and purified water is 1:2000.

[0010] Preferably, the crop early growth promoter is used to promote the growth of crop roots, stems and leaves and increase crop yield.

[0011] Preferably, the crop includes any one of wheat, lettuce and white radish.

[0012] Preferably, the crop early growth promoter is used to promote the growth of wheat roots / stems / leafs, improve the water holding capacity of wheat roots, increase the fresh weight of wheat grains and enhance wheat yield.

[0013] Preferably, the crop early growth promoter is used to promote the fresh weight of lettuce stems / leaves, enhance the water absorption capacity of the root system, and enhance the net photosynthetic rate of the plant.

[0014] Preferably, the crop early growth promoter is used to promote the fresh weight of white radish stems / leaves, increase the length of roots, and increase the width of roots.

[0015] A crop early growth promoter contains the Vishnyk yeast species.

[0016] Compared with the prior art, the present invention has at least the following technical effects:

[0017] (1) The present invention provides a use of a Vishnyk yeast strain in the preparation of an early crop growth promoter. The Vishnyk yeast strain can be dissolved in pure water and mixed thoroughly before direct application. Indoor experimental results show that the yeast significantly promotes the fresh weight growth of stems and leaves of wheat, lettuce, and white radish after application, with the best effect achieved at a concentration of 1:2000. In field experiments, the yeast agent has a stable effect on increasing wheat yield (12.6%), increasing lettuce fresh weight (35%), and white radish root development (23% fresh weight increase), and can indirectly enhance crop yield and quality by improving photosynthetic efficiency (in the case of lettuce) and stress resistance (in the case of wheat).

[0018] (2) This Vishnyk yeast strain promoted the stems and leaves of three crops to a certain extent in the indoor controlled experiment, indicating that Vishnyk yeast may promote the early growth of crops by improving water absorption or regulating plant hormone levels.

[0019] In outdoor field experiments, the presence of Vishnyk yeast significantly increased not only wheat biomass but also grain yield, demonstrating a dual benefit, not only by promoting plant growth but also increasing fruit yield. Both fresh and dry matter mass of lettuce were significantly improved. Combined with the net photosynthetic efficiency of its leaves, it is speculated that Vishnyk yeast may be promoting crop growth by altering the photosynthetic efficiency of its leaves, thereby increasing crop biomass accumulation. The root length and fresh weight of white radish were significantly increased, with the average fresh weight of the experimental group increasing by 23% compared to the control group. The inoculum in natural soil environments is more conducive to root expansion and nutrient absorption. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a comparison chart of wheat fresh matter and dry matter;

[0021] Figure 2 This is a comparison chart of the weight of every 200 wheat plants and the weight of wheat kernels;

[0022] Figure 3 This is a comparison chart of fresh matter and dry matter of lettuce in indoor experiments;

[0023] Figure 4 This is a comparison chart of fresh matter and dry matter of lettuce in field experiments;

[0024] Figure 5 is the net photosynthetic rate of lettuce;

[0025] Figure 6 This is a comparison chart of fresh matter and dry matter of white radish in indoor experiments;

[0026] Figure 7 This is a comparison chart of fresh matter and dry matter of white radish in field experiments;

[0027] Figure 8 This is a comparison chart of the root growth of white radish. DETAILED DESCRIPTION

[0028] The embodiments of the present invention will be described in detail below with reference to the examples. However, those skilled in the art will understand that the following examples are only used to illustrate the present invention and should not be construed as limiting the scope of the present invention. Specific conditions not specified in the examples are carried out according to conventional conditions or conditions recommended by the manufacturer. Reagents or instruments used without indicating the manufacturer are all conventional products that can be purchased commercially.

[0029] Experimental example:

[0030] 1. Experimental Content

[0031] 1. Indoor Experiment

[0032] Wheat, lettuce and white radish were selected as research objects, and the cultured and collected strains were dissolved in pure water to set up five different concentration gradients, namely CK (no bacterial agent added), 1:500 group, 1:1000 group, 1:2000 group and 1:4000 group.

[0033] The wheat was cultured in soil for 14 days, and all the planted wheat samples were harvested after 14 days.

[0034] Lettuce and radish were hydroponically grown for 7 days, and all hydroponic lettuce and radish samples were harvested after 7 days.

[0035] The samples collected from each group were statistically analyzed and compared for biomass, and the differences in biomass of crops in each group relative to the CK group after adding the fungicide were studied.

[0036] 2. Field trials

[0037] Wheat, lettuce and white radish were selected as research objects. The most suitable concentration of microbial agent was selected according to the research results of indoor experiments. After each crop matured, it was harvested and statistically compared with the CK group to study the effect of microbial agent on the early growth of crops in a normal growth environment.

[0038] 2. Research Methods

[0039] 1. Indoor research

[0040] 1.1 Cultivation of Vishnyk yeast strains:

[0041] The solid culture process of Vishnyk yeast generally includes the following steps:

[0042] (1) Culture medium preparation: Choose a commonly used solid culture medium: yeast extract peptone dextrose (YPD) medium for culturing Vishnyk yeast. The formula of this solid culture medium is 10g yeast extract, 20g peptone, 20g glucose, and 15-20g agar powder. Add 1L of agar powder. Mix all the ingredients evenly, heat and stir to completely dissolve the agar, and then sterilize. High-pressure steam sterilization is generally used at 121℃ for 15-20 minutes.

[0043] (2) Inoculation: Under sterile conditions, inoculate the Vishnyk yeast strain onto a solid culture medium. Use an inoculation loop or pipette to evenly spread or streak the yeast cells on the surface of the culture medium.

[0044] (3) Cultivation: Place the inoculated culture medium in a constant temperature incubator for cultivation. The growth temperature of Vishnyk yeast is generally around 25-30°C. The incubation time depends on the specific situation and is usually 2-5 days, until obvious yeast colonies appear on the culture medium. During the incubation process, pay attention to maintaining humidity and ventilation in the incubator to prevent the culture medium from drying out.

[0045] (4) Observation and recording: During the culture process, regularly observe the growth of the yeast colonies, including the morphology, size, color and other characteristics of the colonies, and keep records.

[0046] (5) Storage: The solid culture of Vishnykia yeast obtained by culture should be stored in a refrigerator at about 4°C for a short period of time so that it can be taken out at any time.

[0047] The culture medium prepared above is sterilized in an autoclave at high temperature and high pressure to prepare a solid plate, the extracted strain is evenly spread on the solid plate, and the strain is cultured using a solid culture method.

[0048] Observe the solid culture medium. When the bacterial colony grows evenly and in good condition, collect the bacterial colony on the culture medium into a 50 ml sterile centrifuge tube for subsequent experiments.

[0049] 1.1.1 The wheat soil culture experiment was set up as follows: CK group (only pure water was added), 1:500 concentration group, 1:1000 concentration group, 1:2000 concentration group, and 1:4000 concentration group. Eight parallel experimental groups were set up in each concentration group to reduce the experimental error caused by accidental occurrence of the experiment.

[0050] Take equal amounts of sandy soil and put them into flower pots for early growth of crops. Add equal amounts of wheat seeds into each flower pot and water it with fungal agents of different concentrations. After the first watering, the next watering should be done every five days.

[0051] When the crops reach the 14th day of early growth, they are photographed and harvested. The harvested wheat plants are divided into three parts: roots, stems, and leaves, and the fresh weight is weighed. The roots, stems, and leaves are then placed in a drying box for drying. After 24 hours, they are taken out and the dry weight is weighed.

[0052] 1.1.2 The hydroponic experiments of lettuce and white radish were set up in the same way, namely CK group (only pure water), 1:500 concentration group, 1:1000 concentration group, 1:2000 concentration group, and 1:4000 concentration group. Two parallel experimental groups were set up for each concentration. 2L of pure water was added to each hydroponic CK group, and 2L of bacterial agent was added to the experimental group. Samples were harvested after seven days.

[0053] The samples were divided into roots and stems, and the fresh weight was weighed. After drying, the dry weight was calculated. The obtained data was collated and statistically analyzed for comparison between different groups to analyze the differences in early crop growth between different groups.

[0054] 2. Field research

[0055] The 1:2000 concentration group, which had the most obvious effect in the indoor experiment, was selected as the experimental concentration for this field study to explore the effect of the fungal agent on the early growth of crops in a normal environment.

[0056] Before conducting the field experiment, sufficient bacterial strains were first cultured in the laboratory for use in the field experiment. The crops used in the field experiment were wheat, lettuce, and white radish. The watering method was drip irrigation. The experiment was divided into a control group and an experimental group. The control group added clean water during the drip irrigation process, and the experimental group added 1:2000 bacterial agent during the drip irrigation process.

[0057] After all three crops were planted, they were watered for the first time, and then watered every other week until they were fully mature. During the planting process, the net photosynthesis rate of the crop leaves was measured using the LI-6800 portable photosynthesis meter to explore whether the photosynthetic rate of the crops changed under the action of the microbial agent.

[0058] When the crops are mature, they are harvested. Each crop is dug out from the field completely, and the roots, stems and leaves of the crops are kept intact as much as possible. After all the crops are harvested, the various indicators of the crops are counted separately.

[0059] The fresh weight of the lettuce roots and leaves is calculated, and then the lettuce is placed in a drying box for drying. After it is completely dried, the dry weight of the lettuce roots and leaves is calculated;

[0060] The root length, root weight, maximum root circumference, and leaf weight of the white radish were calculated, and then the white radish was dried and the dry weight of its roots and leaves was calculated;

[0061] The wheat was grouped into groups of 200, and the total weight of the wheat and the weight of the wheat kernels produced were counted separately.

[0062] 3. Analysis of experimental results

[0063] 3.1 Analysis of wheat biomass impact

[0064] 3.1.1 Indoor research and analysis

[0065] The concentration gradient mentioned above is 1:4000 (2×10 8 viable bacteria / ml) as T1 group, 1:2000 (4×10 8 viable bacteria / ml) was recorded as T2, 1:1000 (8×10 8 viable bacteria / ml) as T3, 1:500 (1.6×10 9 viable bacteria / ml) was recorded as T4.

[0066] like Figure 1The following is a comparison chart of wheat fresh matter and dry matter. A is a comparison chart of wheat fresh matter weight; B is a comparison chart of wheat dry matter weight.

[0067] Combining the results Figure 1 As shown in A, among all experimental groups, the fresh weight of wheat roots in all experimental groups was higher than that in the control group.

[0068] The root fresh weight of the T2 treatment group showed a significant advantage compared with the control group CK.

[0069] In terms of the fresh weight of stems and leaves, except for the T4 experimental group, the fresh weight of stems and leaves of the other experimental groups were significantly higher than those of the control group.

[0070] Combining the results Figure 1 As shown in Figure B, the dry matter weight of wheat roots and stems in the experimental group did not show a significant increase compared to the control group. However, the dry matter weight of leaves in the experimental groups T1 and T2 was significantly higher than that in the control group CK.

[0071] comprehensive Figure 1 Based on the data presented in Figures A and B, we can conclude that Vishnyk can effectively promote the growth of wheat roots, stems, and leaves to a certain extent. In particular, it can significantly increase the water-holding capacity of wheat roots.

[0072] Further analysis found that the growth-promoting ability of Vishny yeast was most significant when the T2 group was treated with a concentration of 1:2000.

[0073] 3.1.2 Field research analysis

[0074] In previous laboratory experiments, comparing various treatment groups revealed that the T2 group demonstrated the most significant improvements in various indicators compared to the control group. In particular, in indicators closely related to wheat growth, given the excellent results demonstrated by the T2 group in laboratory experiments, to further explore the potential of this treatment in actual production environments, it was decided to conduct field experiments using the corresponding 1:2000 concentration of the T2 group. In the field experiments, the area treated with this concentration of the inoculant was designated as the experimental group, along with a corresponding control group.

[0075] like Figure 2 The following is a diagram showing the weight of 200 wheat plants and the weight of wheat grains. A is a diagram showing the weight of 200 wheat plants; B is a diagram showing the weight of 200 wheat grains.

[0076] Depend on Figure 2It can be found from A that in the field experiment, the fresh weight of wheat in the experimental group with the addition of 1:2000 concentration of microbial agent was still significantly higher than that in the control group. It can be seen that the growth-promoting effect of microbial agent on wheat is not only applicable to the controlled experiment in the indoor stage, but can also play a certain growth-promoting role in the normal natural environment. The microbial agent shows a certain adaptability to the environment.

[0077] Depend on Figure 2 As can be clearly seen in Figure B, the wheat grain yield in the experimental group was significantly higher than that in the control group. This indicates that the inoculant's effect on wheat is not limited to the wheat plants themselves, but also increases the fruit yield to a certain extent. This indicates that Vishnyk yeast not only promotes wheat growth but also increases crop yield, reflecting the diversity of the inoculant's effects on crops.

[0078] 3.2 Analysis of the impact of lettuce biomass

[0079] 3.2.1 Indoor research analysis

[0080] like Figure 3 The following is a comparison chart of the fresh matter and dry matter of lettuce in an indoor experiment. In the figure, A is a comparison chart of the fresh matter weight of lettuce; B is a comparison chart of the dry matter weight of lettuce.

[0081] Depend on Figure 3 As shown in A, after 7 days of hydroponic experiment, the fresh weight of roots in the experimental group increased compared with the control group but not significantly, while the fresh weight of stems and leaves was significantly higher than that of the experimental group, among which the T2 experimental group was the most significant.

[0082] Depend on Figure 3 As shown in Figure B, the dry weight of lettuce, whether roots or stems and leaves, did not change significantly compared with the experimental group. This may be due to the fact that the cultivation time was too short and the dry matter was not accumulated.

[0083] Combine Figure 3 From A and B, we can infer that the addition of fungicides may play an important role in the early growth stage of crops, especially in promoting the absorption of water by crops.

[0084] In hydroponic environments, efficient water absorption during the early stages of crop growth is crucial for overall plant growth and development. The presence of microbial inoculations may improve the physiological state of crop roots and enhance their affinity for water, thereby enhancing the crop's ability to absorb water during these early stages, thus laying a good foundation for subsequent growth. Although a significant improvement in dry weight has not yet been demonstrated, the significant increase in fresh weight, particularly stem and leaf fresh weight, indirectly confirms the positive impact of microbial inoculations during the early stages of crop growth.

[0085] 3.2.2 Field research analysis

[0086] like Figure 4 The following is a comparison of the fresh matter and dry matter of lettuce in a field experiment. A is a comparison of the fresh matter weight of lettuce; B is a comparison of the dry matter weight of lettuce.

[0087] like Figure 5 Shown is a comparison chart of the net photosynthetic rate of lettuce.

[0088] Depend on Figure 4 It can be seen that compared with the control group, the lettuce in the experimental group with added Vishnu yeast achieved significant growth results. Figure 4 In Figure A, the lettuce in the experimental group not only achieved a significant increase in fresh matter weight; in Figure B, its dry matter weight also increased significantly.

[0089] Recombination Figure 5 This suggests that Vishnyk yeast may have significantly improved the efficiency of physiological processes such as carbohydrate synthesis and accumulation by increasing the net photosynthetic rate of lettuce.

[0090] This dual growth trend in both fresh and dry matter weight comprehensively and fully demonstrates the remarkable effectiveness of Vishnyk yeast as a microbial inoculant in promoting lettuce growth. It is highly likely that this synergistically promotes lettuce growth and development through a complex mechanism, including regulating nutrient absorption efficiency by the lettuce roots, improving the rhizosphere microenvironment, and stimulating hormonal balance within the plant, resulting in biomass accumulation and biomass synthesis exceeding those of the naturally grown control group.

[0091] Table 1 Statistics of fresh lettuce weight

[0092] Maximum weight (g) Minimum weight (g) Average weight (g) control group 509.53 66.1 263.87 Experimental group 727.6 94.67 356.26

[0093] Table 2 Statistics of dry matter weight of lettuce

[0094] Maximum weight (g) Minimum weight (g) Average weight (g) control group 27.6 3.32 14.68 Experimental group 40.4 2.18 17.98

[0095] According to the detailed data analysis in Tables 1 and 2 above, it can be clearly seen that in the experimental group with the addition of bacterial agents, the fresh matter weight of lettuce showed significant superiority.

[0096] The fresh weight of lettuce in the experimental group not only far exceeded that of the control group without added microbial agents in terms of its maximum value, but also showed a significant advantage in terms of the minimum value, which shows that the introduction of microbial agents has a universal and stable effect in promoting the growth of lettuce.

[0097] Furthermore, calculations of average fresh weight revealed a 35% increase in the experimental group compared to the control group. This significant increase not only demonstrates the direct effect of the inoculant on lettuce growth but also suggests potential positive impacts on nutrient absorption and stress resistance. The increase in lettuce dry matter further demonstrates the significant role of the inoculant in promoting biomass accumulation in the lettuce.

[0098] 3.3 Analysis of the impact of white radish biomass

[0099] 3.3.1 Indoor research analysis

[0100] like Figure 6 The following is a comparison chart of fresh matter and dry matter of white radish in an indoor experiment. A is a comparison chart of fresh matter weight of white radish; B is a comparison chart of dry matter weight of white radish.

[0101] Combine Figure 6 It can be seen from A and B in the figure that the bacterial agent did not significantly improve the roots and dry matter accumulation of white radish in the early growth stage, but showed a significant promoting effect on the growth of its stems and leaves, and promoted its roots and stems to obtain water. Its effect showed a certain similarity with that of hydroponic lettuce.

[0102] 3.3.2 Field research analysis

[0103] like Figure 7 The following is a comparison chart of fresh matter and dry matter of white radish in field experiments. A is a comparison chart of fresh matter weight of white radish; B is a comparison chart of dry matter weight of white radish.

[0104] Depend on Figure 7 As shown in Figures A and B, the inoculum also had an effect on the growth of white radish, with both its fresh and dry matter weights increasing to varying degrees. The increase in fresh matter weight directly indicates that the inoculum enabled the radish to better absorb and utilize water and nutrients from the soil, promoting rapid cell expansion and tissue growth. The increase in dry matter weight further demonstrates the inoculum's unique role in boosting the radish's photosynthetic efficiency and enhancing its ability to synthesize and accumulate substances.

[0105] Table 3 Statistics of white radish roots

[0106] Maximum root circumference Maximum root length Minimum root length Average root length control group 29.4 51 23 32.45 Experimental group 29.2 74.6 26 36.67

[0107] like Figure 8 Shown is a comparison chart of the root growth of white radish.

[0108] Depend on Figure 8As shown in Table 3, the effect of the inoculant on white radish root growth is not limited to a single aspect. It is reflected in both a significant extension of root length and an effective increase in root width. The combined effect of these two factors has a positive effect on the growth of white radish.

[0109] Table 4 Statistics of fresh weight of white radish

[0110] Maximum weight (g) Minimum weight (g) Average weight (g) control group 1493.79 213.52 952.45 Experimental group 2124.72 321.34 1176.21

[0111] Combined with Table 4, it can be seen that the fresh weight of white radish in the experimental group not only far exceeded that of the control group without the addition of the microbial agent in its maximum value, but also showed a greater advantage in the minimum value, which shows that the introduction of the microbial agent has a universal and stable effect on the growth of white radish.

[0112] After calculation, the weight of the white radish in the experimental group with added bacterial agent increased by about 23% compared with the white radish in the experimental group.

[0113] 4. Experimental Analysis and Summary

[0114] 4.1 Indoor research

[0115] In indoor controlled experiments, Vishny yeast showed different degrees of promoting effect on the growth of wheat, lettuce and white radish. The different promoting effects may be caused by the differences between crops.

[0116] In summary, Vishny's yeast had a certain degree of promoting effect on the stems and leaves of the three crops in the indoor controlled experiment, indicating that Vishny's yeast may promote the early growth of crops by improving water absorption or regulating plant hormone levels.

[0117] However, there was no significant change in the accumulation of dry matter in crops. The reason may be that the cultivation time in the indoor controlled experiment was too short, and the crops did not have enough time to grow. In addition, in the indoor environment, the crops could not get enough light, photosynthesis was restricted, and dry matter could not be fully accumulated, which had a certain impact on the results of the indoor experiment. However, Vishnyk yeast did show that it had a certain promoting effect on the growth of wheat, lettuce, and white radish.

[0118] 4.2 Field research

[0119] In field experiments, under the action of Vishnicki yeast, not only the biomass of wheat was significantly increased, but its grain yield was also significantly increased. This shows that it can not only promote plant growth, but also increase fruit yield, which has dual benefits.

[0120] The fresh matter and dry matter quality of lettuce were significantly improved. Combined with the net photosynthetic efficiency diagram of its leaves, it can be inferred that Vishnyk yeast may promote crop growth by changing the photosynthetic efficiency of its leaves to achieve the accumulation of crop biomass.

[0121] The root length and fresh weight of white radish were significantly improved. The average fresh weight of the experimental group increased by 23% compared with the control group. The bacterial agent was more conducive to root expansion and nutrient absorption in the natural soil environment.

[0122] In summary, the above-mentioned microbial agent's effect on crops continued to be significant beyond the stable, controlled environment of indoor experiments and into the more complex environment of the field, demonstrating its good stress resistance. Furthermore, its effect on three completely different types of crops demonstrated its considerable applicability.

[0123] 5. Experimental Conclusions

[0124] 5.1 Environmental adaptability

[0125] Laboratory experiments showed that the yeast significantly promoted the fresh weight growth of wheat, lettuce, and white radish stems and leaves (with the best effect at a concentration of 1:2000). However, its effect on dry matter accumulation was limited by the experimental period and light conditions. In field experiments, the yeast inoculant had a stable effect on increasing wheat yield (12.6%), increasing lettuce fresh weight (35%), and white radish root development (23% fresh weight increase). It also indirectly enhanced crop yield and quality by improving photosynthetic efficiency (in the case of lettuce) and stress resistance (in the case of wheat).

[0126] 5.2 Output Value Added

[0127] Field experiments showed that the grain yield of the experimental group increased by 12.6% compared with the control group ( Figure 2 B). Based on the 2023 wheat market price of 2.5 yuan / kg, assuming a per-acre yield of 400 kg, the experimental group saw a 50.4 kg yield increase, with an income increase of 126 yuan / mu. The lettuce experimental group saw an average 35% increase in fresh weight (Table 1), calculated at a market price of 3 yuan / kg. If the per-acre yield of fresh vegetables reached 800 kg (the control group), the experimental group saw a 280 kg yield increase, with an income increase of 840 yuan / mu. The experimental group saw an average 23% increase in fresh weight (Table 4), calculated at a market price of 2 yuan / kg. If the per-acre yield of fresh radishes reached 1,200 kg (the control group), the experimental group saw a 276 kg yield increase, with an income increase of 552 yuan / mu.

[0128] This study demonstrates the great potential of Vishny yeast in promoting early crop growth, improving economic benefits and ecological protection, and can serve as a powerful tool for green agricultural transformation.

[0129] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the scope of protection of the present invention.

Claims

1. A use of a Vishnyk yeast strain in preparing an early growth promoter for crops, characterized in that: The crop early growth promoter is prepared by dissolving the cultured Vishnyk yeast in pure water and mixing it evenly. The mass concentration of the mixture of the Vishnyk yeast strain and purified water is 1:500 to 4000.

2. The use of a Vishnyk yeast strain in the preparation of a crop early growth promoter according to claim 1, characterized in that: The mass concentration of the mixture of the Vishnyk yeast strain and purified water is 1:2000.

3. The use of a Vishnyk yeast strain in the preparation of a crop early growth promoter according to claim 1, characterized in that: The crop early growth promoter is used to promote the growth of crop roots, stems and leaves and increase crop yield.

4. The use of a Vishnyk yeast strain in preparing an early crop growth promoter according to claim 1, characterized in that: The crop includes any one of wheat, lettuce and white radish.

5. The use of a Vishnyk yeast strain in preparing an early crop growth promoter according to claim 4, characterized in that: The crop early growth promoter is used in promoting the growth of wheat roots / stems / leaves, improving the water holding capacity of wheat roots, increasing the fresh weight of wheat grains and improving wheat yield.

6. The use of a Vishnyk yeast strain in preparing an early crop growth promoter according to claim 4, characterized in that: The crop early growth promoter is used in promoting the fresh weight of lettuce stems / leaves, improving the water absorption capacity of the root system, and improving the net photosynthetic rate of the plant.

7. The use of a Vishnyk yeast strain in preparing an early crop growth promoter according to claim 4, characterized in that: The crop early growth promoter is used in promoting the fresh weight of white radish stems / leaves, increasing the extension of root length, and increasing the root width.

8. A crop early growth promoter, characterized in that: The crop early growth promoter contains the Vishnyk yeast species according to claim 1.

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