Plant polypeptide beverage with functions of dispelling effects of alcohol and protecting liver as well as preparation method and application of plant polypeptide beverage

Abstract

The invention provides a plant polypeptide beverage with functions of dispelling effects of alcohol and protecting liver as well as a preparation method and application of the plant polypeptide beverage. By compounding the corn oligopeptide powder, the wheat oligopeptide powder, the concentrated apple clear juice, the radix puerariae powder, the hovenia dulcis thunb powder and other natural components, a synergistic interaction system is constructed; the corn / wheat oligopeptide provides amino acid complementation and activates ADH and ALDH of the liver; the radix puerariae powder and the semen hoveniae powder inhibit acetaldehyde accumulation; the concentrated apple clear juice, the acerola cherry powder and the wolfberry powder enhance the antioxidant activity. During preparation, high-pressure homogenization and UHT instantaneous sterilization processes are adopted, and polydextrose is combined to form a stable colloid. Experiments show that the beverage can improve the ethanol metabolism rate and reduce the levels of ALT and AST in serum. In addition, the levels of SOD, GSH-Px and GSH in the liver are increased, the antioxidant capacity of the liver is improved, damage of ethyl alcohol to the liver is relieved, and the beverage has taste palatability and component stability and is suitable for dispelling the effects of alcohol and preventing alcoholic liver injury.

Description

Technical Field

[0001] The present invention belongs to the technical field of functional foods and relates to a hangover-relieving beverage, and more specifically to a plant polypeptide beverage with hangover-relieving and liver-protecting functions, a preparation method thereof, and the application of the beverage in hangover-relieving and preventing alcoholic liver damage. Background Art

[0002] With increasing social interaction, health issues related to alcohol consumption are becoming increasingly prominent. Acetaldehyde and reactive oxygen species (ROS) produced during ethanol metabolism can trigger oxidative stress in the liver, manifesting as significant increases in liver damage markers such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST), along with decreased activity of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), thus causing liver damage.

[0003] Current hangover remedies rely on chemically synthesized ingredients or single plant extracts, which pose challenges such as low absorption rates and the risk of side effects. They can only alleviate the feeling of intoxication but fail to balance the oxidative stress caused by excessive alcohol intake. Furthermore, most alcohol remedies currently available in China are traditional Chinese medicine (TCM) compound formulas, which have complex ingredients, making it difficult to identify the specific active components and controlling the effective dosage.

[0004] Research has shown that corn oligopeptides (rich in alanine and glutamic acid) and wheat oligopeptides (high in branched-chain amino acids) can accelerate ethanol metabolism by activating the liver's alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). For example, application CN 114468046 A combines corn oligopeptide powder and wheat oligopeptide powder with mixed probiotics to create a hangover-relief, liver-protecting, and stomach-protecting preparation. While this product accelerates ethanol metabolism, it lacks antioxidant capacity and fails to effectively protect the liver. Furthermore, this product lacks attention to flavor, retaining the inherent bitterness of the peptide extract, making it difficult for intoxicated individuals to accept. Summary of the Invention

[0005] In order to solve the problems of the existing hangover products lacking antioxidant stress resistance and poor taste, the present invention provides a plant polypeptide beverage with hangover and liver protection functions, as well as a preparation method and application thereof. By compounding multiple natural ingredients to construct a synergistic enhancement system and combining it with a specific processing technology, the problem of efficacy limitations in the existing technology is solved.

[0006] The technical solution adopted by the present invention is: a plant polypeptide beverage with the function of sobering up and protecting the liver, comprising the following raw materials in parts by weight: 50-75 parts of water, 30-43 parts of plant oligopeptide powder, 15-35 parts of antioxidant components, 4-10 parts of polydextrose, and 0.1-0.5 parts of food-grade pH regulator; the plant oligopeptide powder comprises at least one of corn oligopeptide powder and wheat oligopeptide powder; the antioxidant component comprises at least one of concentrated apple juice, kudzu root powder, hovenia dulcis powder, acerola cherry powder, and wolfberry powder.

[0007] Preferably, the plant oligopeptide powder includes 20-25 parts of corn oligopeptide powder and 10-18 parts of wheat oligopeptide powder in parts by weight.

[0008] Preferably, the antioxidant component comprises, by weight, 8-15 parts of concentrated apple juice, 3-8 parts of kudzu root powder, 2-6 parts of hovenia dulcis powder, 1-3 parts of acerola cherry powder, and 1-3 parts of wolfberry powder.

[0009] Preferably, the mass ratio of the plant oligopeptide powder to the antioxidant component is (1.2-1.4):1, more preferably 1.3:1.

[0010] Preferably, the mass ratio of the corn oligopeptide powder to the wheat oligopeptide powder is (1.1-2.5):1, more preferably 1.2-1.5:1.

[0011] Preferably, the grade pH regulator includes at least one of citric acid or malic acid.

[0012] The present invention also provides a method for preparing the plant polypeptide beverage, comprising the following steps: Step 1: Dry mix corn oligopeptide powder, wheat oligopeptide powder and polydextrose, add water to dissolve, and stir evenly; Step 2: Add kudzu root powder, Hovenia dulcis powder, acerola cherry powder, and wolfberry powder and homogenize under high pressure; Step 3: Add concentrated apple juice, adjust pH, and sterilize by UHT; Step 4: Aseptic filling and cooling to obtain the plant polypeptide beverage.

[0013] Preferably, in step 2, the high-pressure homogenization condition is 30-50 MPa for 2-4 times.

[0014] Preferably, in step 3, the pH is adjusted to 3.5-4.2.

[0015] Preferably, in step 3, the UHT instantaneous sterilization condition is 135-140° C. for 5-10 seconds.

[0016] The present invention also provides the use of the plant polypeptide beverage in sobering up and / or preventing alcoholic liver damage.

[0017] Beneficial effects of the present invention: The plant-based peptide beverage provided by the present invention promotes ethanol metabolism, enhancing liver detoxification and antioxidant capacity, thereby achieving alcohol detoxification and liver protection. Corn oligopeptides (rich in alanine and glutamic acid) and wheat oligopeptides (highly branched-chain amino acids) form complementary amino acid profiles, activating the liver's ADH and ALDH, accelerating ethanol metabolism and reducing blood alcohol concentration, thereby alleviating liver burden. Kudzu root powder (rich in puerarin) and Hovenia dulcis (rich in dihydromyricetin) powder inhibit acetaldehyde accumulation and alleviate liver damage. Concentrated apple juice (rich in apple polyphenols) not only improves the product's flavor and reduces the bitterness of peptides, but also synergizes with acerola cherry powder (rich in vitamin C) and wolfberry powder (rich in wolfberry polysaccharides) to exert a powerful antioxidant effect, boosting liver SOD and GSH-PX activity, enhancing liver antioxidant capacity, and alleviating liver damage caused by alcohol consumption. These ingredients synergistically enhance alcohol detoxification and liver protection, while simultaneously enhancing metabolism and antioxidant levels, reducing damage. This product has the effects of sobering up and protecting the liver, and can be used as a health food with good effects in preventing or treating alcoholic liver damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Are the levels of ethanol, AST and ALT in mouse serum; ### compared with the normal group, p < 0.001; ## compared with the normal group, p < 0.01; **** compared with the model group, p < 0.001; compared with the model group, p < 0.01.

[0019] Figure 2 Are the levels of ADH and ALDH in mouse liver; ### compared with the normal group, p < 0.001; # compared with the normal group, p < 0.05; ** compared with the model group, p < 0.01; * compared with the model group, p < 0.05.

[0020] Figure 3 Statistical results of SOD, GSH and GSH-PX levels in the liver of mice in each group; ### compared with the normal group, p < 0.001; ## compared with the normal group, p < 0.01; *** compared with the model group, p < 0.001; ** compared with the model group, p < 0.01. DETAILED DESCRIPTION

[0021] The following describes the embodiments of the present invention by specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments. The various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that, in the case of no conflict, the features in the following examples and embodiments can be combined with each other. In the embodiments of the present invention, unless otherwise specified, the methods used are all conventional methods, and the reagents used can be obtained from commercial sources.

[0022] Example 1 This embodiment provides a plant polypeptide drink comprising the following components: 55 parts of purified water, 34 parts of wheat oligopeptide powder, 12 parts of concentrated apple juice, 5 parts of kudzu root powder, 4 parts of hovenia dulcis powder, 2.5 parts of acerola cherry powder, 2 parts of wolfberry powder, 8 parts of polydextrose, and 0.5 parts of malic acid.

[0023] The preparation method of the above-mentioned beverage includes the following steps: dry-mixing corn oligopeptide powder, wheat oligopeptide powder and polydextrose, adding 50-60°C purified water to dissolve, and stirring until uniform; adding kudzu root powder, hovenia dulcis powder, acerola cherry powder, and wolfberry powder in sequence, and homogenizing at 40MPa high pressure for 3 times; adding concentrated apple juice, adjusting the pH to 3.5-4.2, and UHT instantaneous sterilization (135°C, 5 seconds); aseptically filling 50 mL / bottle, cooling to below 25°C, and obtaining the final product, which is a light orange transparent liquid with a pH of 3.8 and a sweet and sour taste.

[0024] Example 2 The present embodiment provides a plant polypeptide beverage comprising the following components: 55 parts of purified water, 34 parts of corn oligopeptide powder, 12 parts of concentrated apple juice, 5 parts of kudzu root powder, 4 parts of hovenia dulcis powder, 2.5 parts of acerola cherry powder, 2 parts of wolfberry powder, 8 parts of polydextrose, and 0.5 parts of malic acid.

[0025] The preparation method of the above beverage is the same as that of Example 1. The finished product is a light orange transparent liquid with a pH of 3.8 and a sweet and sour taste.

[0026] Example 3 The present embodiment provides a plant polypeptide beverage comprising the following components: 55 parts of purified water, 20 parts of corn oligopeptide powder, 14 parts of wheat oligopeptide powder, 12 parts of concentrated apple juice, 5 parts of kudzu root powder, 4 parts of hovenia dulcis powder, 2.5 parts of acerola cherry powder, 2 parts of wolfberry powder, 8 parts of polydextrose, and 0.5 parts of malic acid.

[0027] The preparation method of the above beverage is the same as that of Example 1. The finished product is a light orange transparent liquid with a pH of 3.8 and a sweet and sour taste.

[0028] Comparative Example 1 This comparative example provides a plant polypeptide drink comprising the following components: 55 parts of purified water, 20 parts of corn oligopeptide powder, and 34 parts of wheat oligopeptide powder.

[0029] The preparation method of the above beverage is the same as that of Example 1. The finished product is a transparent liquid with a pH of 6.0, and has a bitter taste that is difficult to accept.

[0030] Application performance of alcohol detoxification and liver protection 1. Experimental Plan Fifty male ICR mice weighing 18±3g were randomly divided into five groups: a normal group, a model group, and Example 1, Comparative Example 1, and Comparative Example 2. The laboratory temperature was 22±2°C, the relative humidity was 55±5%, and the light-dark cycle was 12 h. Water and food were freely available. Starting on day 1, mice in the normal and model groups were gavaged daily with normal saline. The other three groups were gavaged daily with 10 mL / kg of Examples 1-3 or Comparative Example 1 for 12 consecutive days. After the last dose on day 13, mice in the normal group were gavaged with normal saline. The other five groups were gavaged with 15 mL / kg of white wine (56% ethanol by volume) with the exception of the normal group, which received normal saline. Two hours after gavage, mice were sacrificed, and serum ethanol, AST, and ALT levels were measured. Liver homogenates were prepared with normal saline to prepare a 10% liver homogenate, and the supernatant was assayed for ADH and ALDH activities, as well as SOD, GSH, and GSH-PX levels according to the kit instructions.

[0031] 2. Experimental Results This experiment measured the ethanol content and liver enzyme levels (ALT, AST) in mouse serum. Figure 1 As shown, compared with the normal group, the serum ethanol content in the model group was extremely significantly increased (p < 0.0001), while the ethanol concentration in the groups treated with the products of Example 1 and Example 3 was significantly reduced compared with the model group (p < 0.001). The serum AST and ALT levels in the model group mice were significantly increased compared with the normal group (p < 0.001), indicating the occurrence of liver damage. Compared with the model group, the ALT and AST levels in the group treated with the product of Example 3 were significantly reduced (p < 0.01); the AST level in the group treated with Example 2 was significantly reduced (p < 0.01); and there was no significant difference in AST and ALT levels in the group treated with Comparative Example 1 compared with the model group. These results indicate that the product of Example 3 has a more pronounced effect in soothing alcohol and protecting against alcoholic liver damage than the product of Comparative Example 1.

[0032] Furthermore, this experiment detected the activity of liver alcohol metabolism enzymes (ADH, ALDH). Figure 2It can be seen that compared with the normal group, the ADH and ALDH activities of the mice in the model group were significantly reduced (p<0.0001; p<0.05); compared with the model group, the ADH activity of the control group 1 and the Example 2-3 treatment groups was significantly increased (p<0.001; p<0.05); compared with the model group, the ALDH activity of the control group 1 and the Example 1-3 treatment groups was significantly increased (p<0.05); the above results indicate that the products of Example 2-3 have a more obvious promoting effect on the activity of alcohol metabolism enzymes than the product of Example 1.

[0033] Furthermore, this experiment detected the levels of liver antioxidant indicators SOD, GSH-PX and GSH. Figure 3 As shown, compared with the normal group, the model group showed significantly lower levels of SOD, GSH, and GSH-PX (p < 0.001), indicating that alcohol consumption significantly impairs the liver's antioxidant capacity. Treatment with the product of Example 3 significantly increased SOD, GSH, and GSH-PX levels (p < 0.001, p < 0.01), demonstrating optimal protection against alcoholic liver damage. In contrast, treatment with the products of Examples 1 and 2 significantly restored only SOD levels compared to the model group (p < 0.01, p < 0.001), with no significant effect on GSH and GSH-PX. Comparative Example 1 also showed no significant antioxidant effect.

[0034] In summary, the product of the present invention can significantly promote alcohol metabolism, increase antioxidant levels in the liver, restore the body's balance between oxidation and antioxidant activity, and alleviate liver damage caused by alcohol intake. These results demonstrate that the plant polypeptide beverage of the present invention has significant effects in sobering up and protecting against alcoholic liver damage, and can be used as a health food with excellent sobering and protective effects against alcoholic liver damage.

[0035] The embodiments described above are merely descriptions of preferred implementations of the present invention and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the scope of protection of the present invention.

Claims

1. A plant polypeptide drink with alcohol sobering and liver protecting functions, characterized in that: The invention comprises the following raw materials in parts by weight: 50-75 parts of water, 30-43 parts of plant oligopeptide powder, 15-35 parts of antioxidant components, 4-10 parts of polydextrose, and 0.1-0.5 parts of food-grade pH regulator; The plant oligopeptide powder includes at least one of corn oligopeptide powder and wheat oligopeptide powder; The antioxidant component includes at least one of concentrated apple juice, kudzu root powder, hovenia dulcis powder, acerola cherry powder, and wolfberry powder.

2. The plant polypeptide beverage according to claim 1, wherein: In parts by weight, the plant oligopeptide powder includes 20-25 parts of corn oligopeptide powder and 10-18 parts of wheat oligopeptide powder.

3. The plant polypeptide beverage according to claim 1, wherein: By weight, the antioxidant component includes 8-15 parts of concentrated apple juice, 3-8 parts of kudzu root powder, 2-6 parts of hovenia dulcis powder, 1-3 parts of acerola cherry powder, and 1-3 parts of wolfberry powder.

4. The plant polypeptide beverage according to claim 1, wherein: The mass ratio of the plant oligopeptide powder to the antioxidant component is (1.2-1.4):

1.

5. The plant polypeptide beverage according to claim 1, wherein: The mass ratio of the corn oligopeptide powder to the wheat oligopeptide powder is (1.1-2.5):

1.

6. A method for preparing the plant polypeptide beverage according to any one of claims 1 to 5, characterized in that: The steps include: Step 1: Dry mix corn oligopeptide powder, wheat oligopeptide powder and polydextrose, add water to dissolve, and stir evenly; Step 2: Add kudzu root powder, Hovenia dulcis powder, acerola cherry powder, and wolfberry powder and homogenize under high pressure; Step 3: Add concentrated apple juice, adjust pH, and sterilize by UHT; Step 4: Aseptic filling and cooling to obtain the plant polypeptide beverage.

7. The preparation method according to claim 6, wherein In step 2, the high-pressure homogenization condition is 30-50 MPa for 2-4 times.

8. The preparation method according to claim 6, wherein In step 3, the pH is adjusted to 3.5-4.

2.

9. The preparation method according to claim 6, wherein In step 3, the UHT instantaneous sterilization conditions are 135-140° C. sterilization for 5-10 seconds.

10. Use of the plant polypeptide beverage according to any one of claims 1 to 5 or the plant polypeptide beverage prepared by the method according to any one of claims 6 to 9 in sobering up and / or preventing alcoholic liver damage.

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