Composition with effect of repairing skin barrier as well as preparation method and application of composition

Abstract

The invention discloses a composition with a skin barrier repairing effect as well as a preparation method and application thereof. The composition comprises carboxymethyl chitosan, ceramide and a centella asiatica extract, the mass ratio of the carboxymethyl chitosan to the ceramide to the centella asiatica extract is (0.01-5): (0.01-5): (0.01-5). The centella asiatica extract comprises one or a combination of more of madecassic acid, a centella asiatica root extract, asiaticoside and madecassoside, and the ceramide comprises one or a combination of more of ceramide NP, ceramide EOP, ceramide AS, ceramide AP, ceramide NS and ceramide NG. By implementing the invention, the skin barrier can be effectively repaired, the sensitivity can be relieved, and the moisturizing and nourishing effects can be realized.

Description

Technical Field

[0001] The present invention relates to the technical field, and in particular to a composition capable of repairing skin barrier function, and a preparation method and application thereof. Background Art

[0002] The skin can protect the human body from physical and chemical damage caused by harmful external factors, but ultraviolet radiation, hormonal changes in the body, improper cleaning and other factors can cause damage to the skin barrier. A damaged skin barrier can not only lead to skin dehydration, dryness, itching, sensitivity, etc., but can even cause skin diseases such as eczema, psoriasis, and acne.

[0003] Skin care products are a good way to repair the skin's barrier function. Currently available moisturizing skin care products usually add natural moisturizing factors such as amino acids, urea, lactate, or lipids such as triglycerides, squalene, ceramide, etc., combined with moisturizers such as sorbitol, lanolin, and vaseline to provide a good moisturizing environment for the skin. However, skin with a damaged barrier is more fragile than normal skin. In addition, the preservatives and fragrances added to skin care products aggravate adverse reactions such as burning, redness, and pain during application. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a composition with the function of repairing the skin barrier, effectively repairing the skin barrier, soothing sensitivity, and moisturizing and nourishing.

[0005] Another technical problem to be solved by the present invention is to provide a method for preparing a composition having the function of repairing the skin barrier.

[0006] Another technical problem to be solved by the present invention is to provide an application of a composition having the function of repairing the skin barrier.

[0007] In order to solve the above problems, the present invention discloses a composition for repairing the skin barrier, which comprises carboxymethyl chitosan, ceramide and Centella asiatica extract; the mass ratio of the carboxymethyl chitosan, ceramide and Centella asiatica extract is (0.01~5):(0.01~5):(0.01~5).

[0008] As an improvement of the above technical solution, the mass ratio of the carboxymethyl chitosan, ceramide and Centella asiatica extract is (0.08-4):(0.05-3):(0.05-3).

[0009] As an improvement to the above technical solution, the Centella asiatica extract is extracted by the following method:

[0010] Reflux extraction is performed on Centella asiatica powder, and filtering is performed to obtain a preliminary extract and a filter residue;

[0011] The preliminary extract is concentrated, freeze-dried, sealed and refrigerated for storage;

[0012] drying and crushing the filter residue, spraying the fermentation liquid to ferment, and filtering to obtain a fermentation product;

[0013] adding a complex enzyme to the fermentation product for enzymolysis, and filtering to obtain a filtrate;

[0014] The filtrate is subjected to enzyme inactivation treatment, crushed and dried to obtain an enzyme inactivation mixture;

[0015] The enzyme-inactivated mixture is mixed with the preliminary extract that is sealed and refrigerated to obtain the product.

[0016] As an improvement to the above technical solution, the solvent used in the reflux extraction is a 60wt% to 80wt% ethanol aqueous solution;

[0017] The mass ratio of the Centella asiatica powder to the solvent is 1:(15-30).

[0018] As an improvement of the above technical solution, the fermentation broth is composed of Mucor and Bifidobacterium;

[0019] The concentration of the fermentation broth is 0.6 wt% to 5 wt%;

[0020] The mass ratio of the Mucor to Bifidobacterium is 1:(0.4-0.7);

[0021] The fermentation time is 24h~72h, and the fermentation temperature is 25℃~35℃.

[0022] As an improvement of the above technical solution, the mass ratio of the fermentation product to the complex enzyme is 1:(0.08-0.15);

[0023] The complex enzyme includes one or more of amylase, acid protease, cellulase, beta-glucosidase and pectinase.

[0024] As an improvement of the above technical solution, the complex enzyme includes cellulase, β-glucosidase and pectinase, and the mass ratio of the cellulase, β-glucosidase and pectinase is 1:(0.3-0.8):(0.5-1);

[0025] The enzymatic hydrolysis time is 1 h to 3 h, the enzymatic hydrolysis temperature is 30° C. to 40° C., and the enzymatic hydrolysis pH is 6 to 7.

[0026] Accordingly, the present invention also discloses a method for preparing the above-mentioned composition, comprising the following steps:

[0027] The Centella asiatica extract and ceramide are uniformly mixed, ultrasonically stirred, and then centrifuged to prepare prefabricated microspheres;

[0028] The prefabricated microspheres are mixed with carboxymethyl chitosan and allowed to stand to obtain the microspheres.

[0029] Correspondingly, the present invention also discloses the use of the above composition in preparing cosmetics with barrier repair and anti-inflammatory and soothing effects.

[0030] Correspondingly, the present invention also discloses a cosmetic containing 0.01 wt% to 15 wt% of the above composition.

[0031] The implementation of the present invention has the following beneficial effects:

[0032] 1. The composition for repairing the skin barrier provided by this invention combines carboxymethyl chitosan to enhance barrier function, ceramides to replenish lipids, and Centella asiatica extract to promote repair. Together, these three ingredients strengthen the skin barrier. Carboxymethyl chitosan locks in moisture, ceramides prevent moisture loss, and Centella asiatica extract provides nutrients. These three ingredients synergistically repair the skin barrier while providing moisturizing and nourishment. Furthermore, carboxymethyl chitosan and Centella asiatica extract possess anti-inflammatory and soothing properties, alleviating skin irritation and sensitivity.

[0033] 2. The present invention first performs reflux extraction on Centella asiatica powder to extract volatile components in flavonoids and polyphenols, then ferments the filter residue, and then uses a complex enzyme to perform enzymatic hydrolysis to convert the glycoside active ingredients into aglycones. Finally, the enzyme-inactivated mixture is mixed with the preliminary extract to obtain the Centella asiatica extract, which retains the active substances in the Centella asiatica to the greatest extent, has high extraction efficiency and stable components of the obtained extract. DETAILED DESCRIPTION

[0034] In order to make the objectives, technical solutions and advantages of the present invention more clear, the present invention is described in further detail below.

[0035] The present invention provides a composition having a skin barrier repairing effect, the composition comprising carboxymethyl chitosan, ceramide and Centella asiatica extract; the mass ratio of the carboxymethyl chitosan, ceramide and Centella asiatica extract is (0.01-5):(0.01-5):(0.01-5).

[0036] In a preferred embodiment, the mass ratio of the carboxymethyl chitosan, ceramide and Centella asiatica extract is (0.08-4):(0.05-3):(0.05-3).

[0037] Carboxymethyl chitosan, a derivative of the alkaline aminopolysaccharide chitin, can be used as a non-hormonal bioactive additive. It is highly safe and has benefits such as immune regulation, anti-inflammatory effects, and wound healing. Compared to chitosan, carboxymethyl chitosan has lower crystallinity and higher water solubility, and its hygroscopicity and moisturizing properties rival those of natural polysaccharides such as hyaluronic acid.

[0038] Ceramide, a major component of intercellular lipids in the skin's stratum corneum, not only acts as a second messenger molecule in the sphingomyelin pathway but also plays a crucial role in the formation of the epidermal stratum corneum. It maintains the skin barrier, provides moisturizing, anti-aging, whitening, and treats diseases. Ceramides include combinations of one or more of ceramide NP, ceramide EOP, ceramide AS, ceramide AP, ceramide NS, and ceramide NG. Ceramide EOP and ceramide NP can repair the sebum membrane and help renew the skin's natural protective layer, enhancing the skin's surface barrier function. Ceramide NS can repair the sebum membrane while inhibiting sebaceous gland secretion, maintaining the skin's water-oil balance. Ceramide AS, ceramide AP, and ceramide NG also have the ability to repair the skin barrier.

[0039] Centella asiatica extract comprises a combination of one or more of madecassic acid, Centella asiatica root extract, asiaticoside, and madecassoside. Triterpenoid saponins such as asiaticoside and madecassoside moisturize and firm the skin. The aglycone in madecassoside, in particular, protects cells from bacterial, fungal, and yeast attacks in natural environments. Furthermore, the polyphenols in Centella asiatica extract possess antioxidant and anti-free radical properties, while the flavonoids possess anti-inflammatory and anti-allergic properties.

[0040] In the composition provided by this invention, carboxymethyl chitosan enhances barrier function, ceramide replenishes lipids, and Centella asiatica extract promotes repair, all three working together to strengthen the skin barrier. Carboxymethyl chitosan locks in moisture, ceramide prevents water loss, and Centella asiatica extract provides nutrients. Together, these three ingredients repair the skin barrier while providing moisturizing and nourishing benefits. Furthermore, carboxymethyl chitosan and Centella asiatica extract have anti-inflammatory and soothing effects, alleviating skin irritation and sensitivity.

[0041] Plant extract skin care products using Centella asiatica extract and other ingredients as raw materials are natural, safe, multifunctional, and environmentally friendly. Among them, the extraction efficiency of plant active ingredients and the stability of active ingredients are crucial to the efficacy of skin care products.

[0042] In a preferred embodiment, the Centella asiatica extract is extracted by the following method:

[0043] S110, performing reflux extraction on the Centella asiatica powder, and filtering to obtain a preliminary extract and filter residue.

[0044] In one embodiment, the solvent used in the reflux extraction is a 60 wt% to 80 wt% ethanol-water solution. Specifically, the mass ratio of the Centella asiatica powder to the solvent is 1:(15-30), exemplified by, but not limited to, 1:18, 1:20, 1:22, 1:24, 1:26, or 1:28. The volatile components of the flavonoids and polyphenols in the Centella asiatica are extracted by reflux, and the triterpenoid saponins in the Centella asiatica are preliminarily extracted.

[0045] S120, concentrating and freeze-drying the preliminary extract, and sealing and refrigerating for storage.

[0046] S130, drying and crushing the filter residue, spraying the fermentation liquid to ferment, and filtering to obtain a fermentation product.

[0047] In one embodiment, the fermentation broth is composed of Mucor and Bifidobacterium, wherein the mass ratio of Mucor to Bifidobacterium is 1: (0.4-0.7), exemplified by 1: 0.42, 1: 0.45, 1: 0.5, 1: 0.55, 1: 0.6 or 1: 0.65, but not limited thereto. Mucor can convert saponin components such as asiaticoside, madecassoside, and asiaticoside B into aglycones, and the short-chain fatty acids (such as butyric acid) produced by bifidobacterium fermentation can enhance the balance of skin microecology, and are suitable for sensitive skin formulas, and are mild and non-irritating. It is understandable that the fermentation of the strain is carried out using MRS culture medium in the art.

[0048] In one embodiment, the concentration of the fermentation broth is 0.6 wt% to 5 wt%, exemplified by, but not limited to, 0.8 wt%, 1 wt%, 1.5 wt%, 2 wt%, 3 wt%, or 4 wt%. A high concentration of the fermentation broth can inhibit bacterial growth and thus affect fermentation efficiency; a low concentration can result in a low yield of the fermentation product.

[0049] Specifically, the fermentation time is 24 hours to 72 hours, the fermentation temperature is 25° C. to 35° C., and the pH is natural.

[0050] S140, adding a complex enzyme to the fermentation product for enzymatic hydrolysis, and filtering to obtain a filtrate.

[0051] In one embodiment, the mass ratio of the complex enzyme to the fermentation product is 1:(0.08-0.15), exemplified by 1:0.09, 1:0.1, 1:0.11, 1:0.12, 1:0.13 or 1:0.14. Too much or too little addition of the complex enzyme will result in reduced enzymatic efficiency.

[0052] Optionally, the complex enzyme includes one or more of amylase, acid protease, cellulase, β-glucosidase, pectinase, papain, and trypsin.

[0053] In a preferred embodiment, the complex enzyme comprises cellulase, β-glucosidase and pectinase, and the mass ratio of the cellulase, β-glucosidase and pectinase is 1:(0.3-0.8):(0.5-1).

[0054] More preferably, the mass ratio of the cellulase, β-glucosidase and pectinase is 1:(0.5-0.7):(0.6-0.8).

[0055] Cellulase can destroy the cell wall skeleton, weaken the rigid structure of the cell wall by decomposing the cellulose network, release active ingredients such as triterpenoids and polysaccharides encapsulated in the cell, and increase the dissolution rate and total amount of the target ingredients.

[0056] β-glucosidase can remove the bottleneck of cellulose decomposition, and at the same time can partially hydrolyze glycosidic bonds, releasing more easily absorbed aglycone forms, thereby enhancing the biological activity of Centella asiatica extract.

[0057] Pectinase can break down the intercellular layer. After pectinase breaks down pectin, the adhesion between cells decreases, promoting cell separation and making it easier to release intracellular components.

[0058] The fermentation product is treated with a complex enzyme of cellulase, β-glucosidase and pectinase, which destroys the cell wall and converts the glycoside components to enhance the activity.

[0059] Specifically, the enzymatic hydrolysis time is 1 to 3 hours, the enzymatic hydrolysis temperature is 30° C. to 40° C., and the enzymatic hydrolysis pH is 6 to 7. The temperature, pH, and time suitable for the complex enzyme reaction are selected to optimize the activity of the complex enzyme and better exert the enzymatic hydrolysis effect of the complex enzyme.

[0060] S150, performing enzyme inactivation treatment on the filtrate, crushing and drying to obtain an enzyme inactivation mixture.

[0061] Specifically, the enzyme inactivation treatment can be performed by heat treatment, pH adjustment, organic solvent precipitation, etc. To maximize the retention of the active ingredients of Centella asiatica, the present invention preferably uses heat treatment at 80°C to 90°C for 8 to 12 minutes. Ultrasonic crushing can be used for crushing for 8 to 15 minutes.

[0062] S160, mixing the enzyme-inactivated mixture with the sealed and refrigerated preliminary extract to obtain.

[0063] The present invention first performs reflux extraction on Centella asiatica powder to extract volatile components in flavonoids and polyphenols, then ferments the filter residue, and then uses a complex enzyme to perform enzymatic hydrolysis to convert cellulose into sugars. Finally, the enzyme-inactivated mixture is mixed with the preliminary extract to obtain the Centella asiatica extract. The active substances in the Centella asiatica are retained to the greatest extent, the extraction efficiency is high, and the components of the extracted extract are stable.

[0064] The present invention also provides a method for preparing the composition, comprising the following steps:

[0065] S210, uniformly mixing the Centella asiatica extract and ceramide, stirring with ultrasound, and then centrifuging to prepare prefabricated microspheres.

[0066] Specifically, the Centella asiatica extract can be extracted using a single alcohol solvent such as isopropyl alcohol or propylene glycol, or an ethanol-water mixed solvent or an ethanol-glycerol mixed solvent, or by supercritical CO2 extraction. In a preferred embodiment, the extraction method of the Centella asiatica extract is as described above.

[0067] S220, mixing the prefabricated microspheres with carboxymethyl chitosan, and allowing the mixture to stand to obtain the microspheres.

[0068] The present invention first uses ceramide to coat the Centella asiatica extract to protect the damaged skin barrier. The carboxymethyl chitosan has active groups such as hydroxyl and amino groups at the end, which form hydrogen bonds with the components of the Centella asiatica extract. The resulting composition has a high skin penetration rate and a stable repair effect.

[0069] Accordingly, the present invention also provides the use of the above composition in preparing a cosmetic having barrier-repairing and anti-inflammatory soothing effects. It is understood that the cosmetic can be a moisturizing cream, a moisturizing lotion, a cream-like face mask, a sheet mask, a facial cleanser, a hand soap, a bath spray, a massage oil, a lip balm, a comfrey ointment, a comfrey stick, a scented powder, a talcum powder, or a prickly heat powder.

[0070] In one embodiment, the cosmetic contains 0.01 wt% to 15 wt% of the above composition. Preferably, the cosmetic contains 1 wt% to 8 wt% of the above composition. The remaining ingredients are excipients, which are conventional commercially available excipients in the art.

[0071] All raw materials used in the present invention are commercially available products in the art.

[0072] The present invention will be further described below with specific embodiments:

[0073] Example 1

[0074] This embodiment provides a composition having the function of repairing the skin barrier, comprising carboxymethyl chitosan, ceramide and Centella asiatica extract, wherein the mass ratio of carboxymethyl chitosan, ceramide and Centella asiatica extract is 5:1:1.

[0075] Carboxymethyl chitosan, ceramide and Centella asiatica extract are mixed evenly according to weight percentage to obtain a composition for repairing the skin barrier.

[0076] Among them, 80wt% ethanol aqueous solution is used to extract Centella asiatica powder, the mass ratio of Centella asiatica powder to solvent is 1:15, after ultrasonication for 1h, the supernatant is filtered and the residue is recycled and extracted once, and then concentrated and dried to obtain Centella asiatica extract.

[0077] Example 2

[0078] This embodiment provides a composition having the function of repairing the skin barrier. The difference between this embodiment and embodiment 1 is that the Centella asiatica extract and ceramide are uniformly mixed according to weight percentage, ultrasonically stirred and then centrifuged to prepare prefabricated microspheres; the prefabricated microspheres are mixed with carboxymethyl deacetylated chitosan and allowed to stand to obtain a composition for repairing the skin barrier.

[0079] The rest are the same as in Example 1.

[0080] Example 3

[0081] This embodiment provides a composition having the function of repairing the skin barrier. The difference between this embodiment and embodiment 2 is that:

[0082] Centella asiatica extract is obtained by the following method:

[0083] S1. Reflux extraction is performed on Centella asiatica powder, and the preliminary extract and filter residue are obtained by filtration.

[0084] A 60 wt % ethanol aqueous solution was used for reflux extraction, and the mass ratio of Centella asiatica powder to the 60 wt % ethanol aqueous solution was 1:30.

[0085] S2. Concentrate and freeze-dry the preliminary extract, seal it and store it in a refrigerator.

[0086] S3, drying and crushing the filter residue, spraying the fermentation liquid to ferment, filtering to obtain a fermentation product, crushing and drying the fermentation product.

[0087] The concentration of the fermentation liquid was 0.6 wt %, and the fermentation liquid consisted of Mucor and Bifidobacterium in a mass ratio of 1:0.4. The fermentation time was 36 h, and the fermentation temperature was 30° C.

[0088] S4. Mixing the crushed and dried fermentation product with the sealed and refrigerated preliminary extract to obtain the product.

[0089] The rest are the same as in Example 2.

[0090] Example 4

[0091] This embodiment provides a composition having the function of repairing the skin barrier. The difference between this embodiment and embodiment 2 is that:

[0092] Centella asiatica extract is obtained by the following method:

[0093] S1. Reflux extraction is performed on Centella asiatica powder, and the preliminary extract and filter residue are obtained by filtration.

[0094] A 60 wt % ethanol aqueous solution was used for reflux extraction, and the mass ratio of Centella asiatica powder to the 60 wt % ethanol aqueous solution was 1:30.

[0095] S2. Concentrate and freeze-dry the preliminary extract, seal it and store it in a refrigerator.

[0096] S3. Adding a complex enzyme to the filter residue for enzymatic hydrolysis, and filtering to obtain a filtrate.

[0097] The mass ratio of the filter residue to the complex enzyme was 1:0.08. The complex enzyme included cellulase, β-glucosidase and pectinase in a mass ratio of 1:0.3:0.5. The enzymolysis time was 1.5 h, the enzymolysis temperature was 35° C., and the enzymolysis pH was 6.5.

[0098] S4, performing enzyme inactivation treatment on the filtrate, crushing and drying to obtain an enzyme inactivation mixture.

[0099] S5. Mix the enzyme-inactivated mixture with the preliminary extract that has been sealed and refrigerated to obtain the product.

[0100] The rest are the same as in Example 2.

[0101] Example 5

[0102] This embodiment provides a composition having the function of repairing the skin barrier. The difference between this embodiment and embodiment 2 is that:

[0103] Centella asiatica extract is obtained by the following method:

[0104] S100, performing reflux extraction on the Centella asiatica powder, and filtering to obtain a preliminary extract and filter residue.

[0105] A 60 wt % ethanol aqueous solution was used for reflux extraction, and the mass ratio of Centella asiatica powder to the 60 wt % ethanol aqueous solution was 1:30.

[0106] S120. Concentrate and freeze-dry the preliminary extract, and store it in a sealed container under refrigeration.

[0107] S130, drying and crushing the filter residue, spraying the fermentation liquid to ferment, and filtering to obtain a fermentation product.

[0108] The concentration of the fermentation liquid is 2 wt %, and the fermentation liquid consists of Mucor and Bifidobacterium in a mass ratio of 1:1. The fermentation time is 36 h and the fermentation temperature is 30° C.

[0109] S140, adding a complex enzyme to the fermentation product for enzymatic hydrolysis, and filtering to obtain a filtrate.

[0110] The mass ratio of fermentation product to complex enzyme is 1:0.15, the complex enzyme includes cellulase, β-glucosidase and pectinase, the mass ratio is 1:1:1, the enzymolysis time is 1.5h, the enzymolysis temperature is 35℃, and the enzymolysis pH is 6.5.

[0111] S150, performing enzyme inactivation treatment on the filtrate, crushing and drying to obtain an enzyme inactivation mixture.

[0112] S160, mixing the enzyme-inactivated mixture with the preliminary extract that is sealed and refrigerated to obtain the product.

[0113] The rest are the same as in Example 2.

[0114] Example 6

[0115] This embodiment provides a composition having the function of repairing the skin barrier. The difference between this embodiment and embodiment 2 is that:

[0116] Centella asiatica extract is obtained by the following method:

[0117] S100, performing reflux extraction on the Centella asiatica powder, and filtering to obtain a preliminary extract and filter residue.

[0118] Reflux extraction was performed using an 80 wt % ethanol aqueous solution, and the mass ratio of Centella asiatica powder to the 80 wt % ethanol aqueous solution was 1:15.

[0119] S120. Concentrate and freeze-dry the preliminary extract, and store it in a sealed container under refrigeration.

[0120] S130, drying and crushing the filter residue, spraying the fermentation liquid to ferment, and filtering to obtain a fermentation product.

[0121] The concentration of the fermentation liquid is 4 wt %, and the fermentation liquid consists of Mucor and Bifidobacterium in a mass ratio of 1:0.5. The fermentation time is 36 h and the fermentation temperature is 30° C.

[0122] S140, adding a complex enzyme to the fermentation product for enzymatic hydrolysis, and filtering to obtain a filtrate.

[0123] The mass ratio of fermentation product to complex enzyme is 1:0.1, the complex enzyme includes cellulase, β-glucosidase and pectinase, the mass ratio is 1:0.5:1, the enzymolysis time is 1.5h, the enzymolysis temperature is 35℃, and the enzymolysis pH is 6.5.

[0124] S150, performing enzyme inactivation treatment on the filtrate, crushing and drying to obtain an enzyme inactivation mixture.

[0125] S160, mixing the enzyme-inactivated mixture with the preliminary extract that is sealed and refrigerated to obtain the product.

[0126] The rest are the same as in Example 2.

[0127] Example 7

[0128] This embodiment provides a composition having the function of repairing the skin barrier. The difference between this embodiment and Example 6 is that the mass ratio of carboxymethyl chitosan, ceramide and Centella asiatica extract is 1:1:1.

[0129] The rest are the same as in Example 6.

[0130] Example 8

[0131] This embodiment provides a composition having the function of repairing the skin barrier. The difference between this embodiment and Example 6 is that the mass ratio of carboxymethyl chitosan, ceramide and Centella asiatica extract is 2:1:1.

[0132] The rest are the same as in Example 6.

[0133] Example 9

[0134] This embodiment provides a composition having the function of repairing the skin barrier. The difference between this embodiment and Example 6 is that the mass ratio of carboxymethyl chitosan, ceramide and Centella asiatica extract is 4:3:3.

[0135] The rest are the same as in Example 6.

[0136] Example 10

[0137] This embodiment provides a composition having the function of repairing the skin barrier. The difference between this embodiment and Example 6 is that the mass ratio of carboxymethyl chitosan, ceramide and Centella asiatica extract is 5:2:2.

[0138] The rest are the same as in Example 6.

[0139] Comparative Example 1

[0140] This comparative example provides a composition having the function of repairing the skin barrier, which is different from Example 1 in that it comprises carboxymethyl chitosan and Centella asiatica extract, and the mass ratio of carboxymethyl chitosan to Centella asiatica extract is 5:1.

[0141] Carboxymethyl chitosan, ceramide and Centella asiatica extract are mixed evenly according to weight percentage to obtain a composition for repairing the skin barrier.

[0142] The rest are the same as in Example 1.

[0143] Comparative Example 2

[0144] This comparative example provides a composition having the function of repairing the skin barrier, which is different from Example 1 in that it comprises ceramide and Centella asiatica extract, and the mass ratio of ceramide to Centella asiatica extract is 1:1.

[0145] Carboxymethyl chitosan, ceramide and Centella asiatica extract are mixed evenly according to weight percentage to obtain a composition for repairing the skin barrier.

[0146] The rest are the same as in Example 1.

[0147] The compositions provided in Example 8 and Comparative Example 2 were mixed with excipients to prepare cosmetics, wherein the mass percentage of the compositions was 1%, thereby obtaining Application Examples 1 and 2. The compositions provided in Example 8 and Comparative Example 2 were mixed with excipients to prepare cosmetics, wherein the mass percentage of the compositions was 5%, thereby obtaining Application Examples 3 and 4.

[0148] Performance testing:

[0149] (1) Evaluation of the soothing effect of the composition

[0150] Hyaluronidase activity inhibition test: prepare four 10 mL stoppered test tubes, named A, B, C, and D, take 0.5 mL of sample solution and add it to A and B respectively, take 0.5 mL of deionized water and add it to C and D respectively, then add 0.5 mL of hyaluronidase solution to A and C respectively, add 0.5 mL of acetate buffer to B and D respectively, then place A, B, C, and D together in an oven at 37°C for 20 min; add 0.25 mmol·L -1 After adding 0.1 mL of sodium chloride solution, place it in an oven and keep it at 37°C for 20 min. Then, add 0.5 mg·mL to A and C respectively. -12mL of sodium hyaluronate was added, and 2mL of acetate buffer was added to each of B and D. The mixture was placed in an oven and kept at 37°C for 20 minutes. After being removed and allowed to stand at room temperature for 10 minutes, 1mL of acetylacetone solution was added to each of A, B, C, and D. The mixture was reacted in boiling water in a water bath for 15 minutes, and then immediately removed and placed in an ice water bath for 15 minutes. After being allowed to stand at room temperature for 10 minutes, Ehrlich's color developer was added to each of A, B, C, and D, and the mixture was thoroughly shaken. 3.0mL of anhydrous ethanol was added for dilution. Finally, the mixture was allowed to stand at room temperature for 40 minutes. The absorbance was measured at 520nm using a UV-visible spectrophotometer. The hyaluronidase activity inhibition rate was calculated according to the following formula:

[0151]

[0152] Where, OD A ,OD B ,OD C ,OD D The absorbance of the solutions in test tubes A, B, C, and D at 520 nm is shown in the table below:

[0153] Hyaluronidase activity inhibition rate Example 1 60.82％ Example 2 62.17％ Example 3 65.46％ Example 4 64.15％ Example 5 71.25％ Example 6 74.26％ Example 7 72.68％ Example 8 74.91％ Example 9 73.96％ Example 10 74.02％ Comparative Example 1 44.81％ Comparative Example 2 45.94％

[0154] (2) Evaluation of moisturizing efficacy of cosmetics

[0155] 30 subjects with normal skin, 15 men and 15 women, aged between 18 and 50 years old, were tested in an environment of 25±2℃ and 60% humidity. All subjects washed their arms with clean water and waited for 30 minutes. Five 4cm*4cm test areas were selected on the inner forearms of both hands of the subjects and marked. Use latex finger cots to evenly apply the samples of Application Example 1 to Application Example 4 to the test areas. The test areas without any samples are blank groups. Use a moisture tester to record the initial value before application, and the water content of the skin stratum corneum 30min, 1h, 2h, 3h, and 4h after application, and calculate the average water content. The specific results are shown in the following table:

[0156]

[0157] (3) Evaluation of cosmetic repair efficacy

[0158] 30 subjects with normal skin, 15 men and 15 women, aged between 18 and 50 years old, were tested in an environment of 25±2℃ and 60% humidity. All subjects washed their arms with clean water and waited for 30 minutes. Five test areas were selected on the inner forearms of both hands of the subjects and marked. 20μL of 1% histamine solution was applied to the patch sticker and stuck to the test area. The sticker was removed after 30 minutes of sticking. The samples corresponding to Application Examples 1 to 4 were evenly applied to the test area using latex finger cots. The test area without any sample was the blank group. The time it took for the erythema and itching to subside at the patch location was recorded. The specific results are shown in the following table:

[0159] Number of people effectively removing redness Number of people whose itching was relieved Number of people feeling stimulated Application Example 1 30 30 0 Application Example 2 24 20 0 Application Example 3 30 30 0 Application Example 4 27 23 0 Blank group 0 0 -

[0160] The above is a preferred embodiment of the invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the invention. These improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. A composition having a skin barrier repairing effect, characterized in that: The composition comprises carboxymethyl chitosan, ceramide and Centella asiatica extract; the mass ratio of the carboxymethyl chitosan, ceramide and Centella asiatica extract is (0.01-5):(0.01-5):(0.01-5).

2. The composition having the effect of repairing the skin barrier according to claim 1, wherein: The mass ratio of the carboxymethyl chitosan, ceramide and Centella asiatica extract is (0.08-4):(0.05-3):(0.05-3).

3. The composition having the effect of repairing the skin barrier according to claim 1, wherein: The Centella asiatica extract is obtained by extraction using the following method: Reflux extraction is performed on Centella asiatica powder, and filtering is performed to obtain a preliminary extract and a filter residue; The preliminary extract is concentrated, freeze-dried, sealed and refrigerated for storage; drying and crushing the filter residue, spraying the fermentation liquid to ferment, and filtering to obtain a fermentation product; adding a complex enzyme to the fermentation product for enzymolysis, and filtering to obtain a filtrate; The filtrate is subjected to enzyme inactivation treatment, crushed and dried to obtain an enzyme inactivation mixture; The enzyme-inactivated mixture is mixed with the preliminary extract that is sealed and refrigerated to obtain the product.

4. The composition having the effect of repairing the skin barrier according to claim 1, wherein: The solvent used in the reflux extraction is 60wt% to 80wt% ethanol aqueous solution; The mass ratio of the Centella asiatica powder to the solvent is 1:(15-30).

5. The composition having the effect of repairing the skin barrier according to claim 1, wherein: The fermentation liquid consists of Mucor and Bifidobacterium; The concentration of the fermentation broth is 0.6 wt% to 5 wt%; The mass ratio of the Mucor to Bifidobacterium is 1:(0.4-0.7); The fermentation time is 24h~72h, and the fermentation temperature is 25℃~35℃.

6. The composition having the effect of repairing the skin barrier according to claim 1, wherein: The mass ratio of the fermentation product to the complex enzyme is 1:(0.08-0.15); The complex enzyme includes one or more of amylase, acid protease, cellulase, beta-glucosidase and pectinase.

7. The composition having the effect of repairing the skin barrier according to claim 1, wherein: The complex enzyme comprises cellulase, β-glucosidase and pectinase, and the mass ratio of the cellulase, β-glucosidase and pectinase is 1:(0.3-0.8):(0.5-1); The enzymatic hydrolysis time is 1 h to 3 h, the enzymatic hydrolysis temperature is 30° C. to 40° C., and the enzymatic hydrolysis pH is 6 to 7.

8. A method for preparing the composition according to any one of claims 1 to 7, characterized in that: The following steps are involved: The Centella asiatica extract and ceramide are uniformly mixed, ultrasonically stirred, and then centrifuged to prepare prefabricated microspheres; The prefabricated microspheres are mixed with carboxymethyl chitosan and allowed to stand to obtain the microspheres.

9. Use of the composition according to any one of claims 1 to 7 in the preparation of cosmetics having barrier repair and anti-inflammatory and soothing effects.

10. A cosmetic, characterized in that: Contains 0.01 wt% to 15 wt% of the composition according to any one of claims 1 to 7.

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