Poison drawing-out and tissue regeneration promoting gel and preparation method thereof

Abstract

The invention discloses a poison drawing-out and tissue regeneration promoting gel and a preparation method thereof, and belongs to the field of pharmaceutics. The gel is prepared from poison drawing-out and tissue regeneration promoting powder, carbomer 940, glycerol, a pH regulator and water, and the stable gel is formed by regulating the pH to 6.0-7.4. The preparation method comprises the steps of gel matrix swelling, medicine dispersing, humectant adding, pH adjusting and homogenizing treatment. By improving the gel dosage form, the diffusion risk of mercury in traditional powder is remarkably reduced, and the drug stability and wound adhesion are improved. Experiments show that the gel can effectively inhibit bacterial biofilm formation and promote collagen synthesis and angiogenesis, and is especially suitable for repairing chronic ulcerative wounds such as burns and scalds.

Description

Technical Field

[0001] The invention belongs to the field of pharmacy and relates to a toxin-eliminating and tissue-promoting gel and a preparation method thereof. Background Art

[0002] Badu Shengji San is a commonly used Chinese medicine for external use. It is composed of borneol, calamine (calcined), dragon bone (calcined), red powder, yellow ochre, calomel, white wax, and gypsum (calcined). It belongs to the category of surgical medicines for removing dead tissue and promoting tissue regeneration. It has the effect of removing toxins and promoting tissue regeneration. It is mainly used for sores with positive symptoms that have ulcerated but the pus and rot have not been cleared and the tissue regeneration has not lasted for a long time. Modern pharmacological research results show that Badu Shengji San has multiple effects such as antibacterial and anti-inflammatory, clearing necrotic tissue, and repairing tissue damage. It can be used to treat chronic ulcerative diseases such as diabetic foot, pressure sores, perianal abscesses, chronic sinus tracts on the body surface, post-traumatic infections, and other ulcers, carbuncles, erysipelas, furuncles, and furuncles. However, this product contains mercury, which has certain safety risks. The dosage form currently used in clinical practice is a powder. When used, the drug is directly sprinkled on the affected area, further increasing the risk of mercury diffusion. For this reason, the present invention improves the dosage form of Badu Shengji San to reduce its safety risks. Summary of the Invention

[0003] The invention aims to provide a detoxification and muscle-stimulating gel, aiming to reduce the safety risks of detoxification and muscle-stimulating powder.

[0004] The present invention provides a detoxifying and tissue-stimulating gel, comprising the following components and their mass percentages: Badu Shengji San: 0.5% to 2%; Gel matrix: 1% to 5%; Moisturizer: 2% to 10%; pH regulator to maintain the pH value of the gel at 6.0 to 7.4; The balance is water, The gel matrix is Carbomer 940.

[0005] Preferably, the humectant is glycerin.

[0006] Preferably, the pH adjuster is triethanolamine.

[0007] Further preferably, the mass percentage of the gel matrix is 5%, the mass percentage of the Badu Shengji San is 2%, the mass percentage of the moisturizer is 5%, and the pH value of the gel is 7.4.

[0008] Furthermore, the gel also includes a preservative, which is methyl parahydroxybenzoate, with a mass percentage of 0.1% to 0.5%.

[0009] The present invention further provides a method for preparing the detoxifying and granulating gel, comprising the following steps: 1) Disperse Carbomer 940 in water and allow it to swell to form a gel matrix; 2) Evenly disperse the Badu Shengji Powder in the gel matrix prepared in step 1); 3) Add glycerin and stir until uniform; 4) Adjust the pH value of the gel to 6.0-7.4; 5) The drug is evenly distributed through homogenization.

[0010] Wherein, the swelling time of Carbomer 940 in step 1) is 24 hours.

[0011] The homogenization treatment in step 5) is performed using a vortex mixer with a rotation speed of 2000 rpm and a mixing time of 10 minutes.

[0012] Experiments have shown that the prepared detoxifying and tissue-regenerating gel has a strong destructive effect on bacterial biofilms, can reduce wound infection, delay skin tissue necrosis and cell apoptosis on the wound surface, promote collagen synthesis and angiogenesis, and has a particularly good promoting effect on the repair of burns and scalds.

[0013] The beneficial effects of the present invention are: 1) It overcomes the risk of drug diffusion in traditional powders and reduces the safety hazards caused by mercury diffusion.

[0014] 2) It improves the efficacy of removing toxins and promoting tissue regeneration, and can better promote wound repair.

[0015] 3) Compared with traditional powders, it is more convenient to use, the dosage is more controllable, and the therapeutic effect is more lasting. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 : Flow behavior of different gel matrices in a horizontal state. From left to right: 1% carbomer; 10% sodium alginate; 5% CMC-Na.

[0017] Figure 2 : Drug dispersion state of sodium alginate gel loaded with Badu Shengji San.

[0018] Figure 3 : Changes in the appearance of 3% Carbomer 940 gel loaded with Badu Shengji San before and after centrifugation. Left: Blank Carbomer 940 gel; Right: Carbomer 940 gel loaded with Badu Shengji San.

[0019] Figure 4 : Changes in properties of drug-loaded hydrogel after being placed in an oven for 48 h.

[0020] Figure 5 : The states of each group of drug-loaded gels prepared according to the orthogonal test table.

[0021] Figure 6 : Changes of the state of detoxification and muscle-generating gel before and after centrifugation.

[0022] Figure 7 : Changes in the state of detoxification and skin regeneration gel before and after high temperature.

[0023] Figure 8 : Changes in the state of detoxification and muscle regeneration gel before and after freezing.

[0024] Figure 9 : Crystal violet staining of bacterial biofilm.

[0025] Figure 10 : SEM observation of the effects of Badushengji gel and Badushengji powder on bacterial biofilm.

[0026] Figure 11 : Photos of rat scald wounds at 0h, 4D, 8D, 14D, and 21D after scald.

[0027] Figure 12 : Hematoxylin and eosin (HE) staining of rat skin after scald.

[0028] Figure 13 : Immunohistochemical staining of rat skin after scald.

[0029] Figure 14 : Sirius red staining of rat skin after scald.

[0030] In the above figures, BDS stands for Badu Shengji San, and BDN stands for Badu Shengji Gel. DETAILED DESCRIPTION

[0031] The present invention will be described in detail below with reference to specific examples. It should be noted that the specific examples are merely for the purpose of explaining the present invention and are not intended to limit the present invention. In addition, the various experimental procedures involved in the examples are conventional in the art unless otherwise stated. For any part not specifically described herein, those skilled in the art can refer to various commonly used reference books, scientific literature, or relevant specifications, manuals, etc. before the filing date of the present invention for implementation.

[0032] The raw materials for Badu Shengji Powder are provided by Yekai Thailand Pharmaceutical (Suizhou) Co., Ltd. of Jianmin Group. It contains 30g of borneol, 72g of calamine (calcined), 72g of dragon bone (calcined), 72g of red powder, 72g of yellow ochre, 72g of calomel, 15g of white wax, and 600g of gypsum (calcined). For the above eight ingredients, except for the borneol, mix the white wax, dragon bone, and gypsum into a very fine powder. The remaining four ingredients, including the red powder, are pulverized into a very fine powder. Grind the borneol finely, mix with the above powders, sieve, mix thoroughly, and package.

[0033] Example 1. Screening of gel formulations 1. Initial Screening of Gel Matrix Ultrapure water was used to prepare 1% and 3% Carbomer 940 blank matrices, 5% and 10% sodium alginate gel matrices, and 3% and 5% CMC-Na gel matrices, respectively. After swelling with water for 24 hours, the properties, centrifugal stability, homogeneity, and fluidity of the blank gel matrices were compared.

[0034] The results showed that Carbomer 940 has a stronger ability to form a gel, forming a uniform and delicate gel at a concentration of 1% (w / v), and the gel prepared at this concentration has moderate fluidity and viscosity. Other gel matrices, on the other hand, have poor formability, fluidity, and viscosity. Even the higher concentrations of 10% sodium alginate and 5% CMC-Na have high fluidity, which is not conducive to effective adhesion and retention on the wound surface. Figure 1 ).

[0035] 2. Gel Matrix Rescreening Badu Shengji Powder contains substances such as mercury, which may interact with the matrix excipients. Alternatively, because Badu Shengji Powder is a hypertonic system, it may cause water to leak out of the gel matrix network, leading to irreversible damage to the gel structure. Therefore, in this study, we conducted a mixed-molding experiment with Badu Shengji Powder gels. A Badu Shengji Powder suspension was prepared using ultrapure water. Subsequently, 1% Carbopol 940 gels, 10% Sodium Alginate gels, and 5% CMC-Na gels were prepared, all with a drug loading of 1 mg / mL. The gel state changes and drug dispersion within the gels were observed.

[0036] Table 1 Gel morphology after adding drug solution

[0037] The experimental results showed that although the Carbomer 940 gel matrix could form a uniform gel in the initial screening test, the addition of Badu Shengji Powder caused the gel to become severely hydrated and the consistency to decrease, but the drug could be mixed evenly in it; although the 10% sodium alginate drug-loaded gel did not hydrate, the powder was difficult to mix evenly in the matrix, and obvious drug precipitation was observed after centrifugation ( Figure 2 ); 5% CMC-Na is difficult to form a gel state with a certain adhesion effect before and after adding the drug.

[0038] 3. Screening of gel matrix dosage According to the results of the matrix initial screening and re-screening, only Carbomer 940 can mix with the drug and form a certain gel state. Therefore, this experiment excluded the other two gel matrices and considered increasing the concentration of Carbomer 940 to alleviate the impact of hydration. Figure 3As shown in the data, increasing the carbomer concentration to 3% can effectively alleviate the hydration caused by the addition of the raw material drug. The drug-loaded gel prepared with carbomer as the matrix is evenly and finely dispersed. The gel changes from the original white transparent state to the light pink of Badu Shengji San. No obvious drug precipitation is observed after centrifugation. Therefore, increasing the proportion of carbomer 940 in the drug-loaded gel is an effective means to improve the gel morphology.

[0039] The experiment also found that while increasing the concentration of Carbomer 940 alleviated the hydration of the drug-loaded gel, the inherent pale pink color of Badu Shengji Powder faded further after the gel was left to stand overnight, and the gel still thinned. We speculate that this may be due to the large number of carboxyl groups in the Carbomer 940 structure, which leads to an acidic gel matrix. This may cause acid hydrolysis of components such as gypsum, calamine, and yellow lead in Badu Shengji Powder, subsequently releasing Ca2+, Hg2+, and Zn2+, which disrupt the gel network and lead to gel hydration. The pale pink color in the gel is likely derived primarily from the yellow lead and red lead components in Badu Shengji Powder. The color loss also indirectly confirms the degradation of yellow lead and red lead. Therefore, it is speculated that adjusting the gel pH may be an effective means of improving the stability of the drug-loaded gel. Therefore, pH adjustment will be considered during the subsequent gel formulation optimization process.

[0040] 4. Screening of moisturizers Using the endpoint moisturizing rate as an evaluation indicator, the type of moisturizer was optimized. Two portions of the preferred drug-loaded gel were prepared, and equal amounts of 5% glycerol and propylene glycol were added to each. The gels were stirred evenly and transferred to a constant-weight petri dish. After weighing, the gels were placed in an oven and dried at 37°C. The petri dishes were removed and weighed at 2, 4, 8, 24, and 48 hours, respectively. The water loss rate (%) was calculated, and changes in the sample appearance at 48 hours were recorded.

[0041] Table 2 Effects of different moisturizers on the water loss rate of carbomer-loaded gel

[0042] As shown in Table 2, under the same time and temperature conditions, glycerol has slightly better moisturizing ability than propylene glycol. After 48 hours in the oven, the endpoint moisturizing rate of the group using glycerol as the moisturizer was 7.2%, while the endpoint moisturizing rate of the group using propylene glycol as the moisturizer was 5.5%. Moreover, compared with propylene glycol, the drug-loaded gel prepared using glycerol as the moisturizer had a smoother and more delicate surface and fewer bubbles ( Figure 4 ), so glycerin is preferably used as the moisturizing agent for preparing the detoxification and muscle-building gel.

[0043] Example 2. Optimization of the formulation of the gel Taking the comprehensive sensory score as the indicator, orthogonal design was used to further optimize the carbomer content, the amount of Badu Shengji Powder added, the pH value and the glycerol content in the gel.

[0044] Table 3 Comprehensive sensory scoring method

[0045] Table 4 Influencing factors and levels of orthogonal experiment

[0046] Table 5 Results of orthogonal test on comprehensive sensory evaluation

[0047] According to the prescription ratio of Table 5, the orthogonal test of detoxification and tissue regeneration gel was prepared, and the prepared gel ( Figure 5 ) were scored, and the orthogonal test results were calculated according to the orthogonal test table. The results are shown in Table 5: Sample No. 9 had the highest overall score, while samples Nos. 1-3 had the lowest overall scores. The range (R) of the orthogonal test results indicates that the carbomer content had the greatest impact on the effect in this experiment, followed by the amount of Badu Shengji San added, followed by the pH value, and the least impacted by the amount of glycerol added. The optimal formula for Badu Shengji Gel is A3B3C1D2, with a Carbomer 940 dosage of 5%, a Badu Shengji San dosage of 2%, a gel pH of 7.40, and a glycerol dosage of 5%.

[0048] Based on the above experiments, the production process of the detoxification and tissue regeneration gel was finally determined as follows: 1. Recipe Badu Shengji San: 2%; Carbomer 940: 5%; Glycerol: 5%; Triethanolamine: to make the pH value of the gel 7.4; Methylparaben: 0.1% The balance is water.

[0049] 2. Preparation method: 1) Matrix preparation: Weigh 50 mg of Carbomer 940 and disperse it in 1 mL of ultrapure water. Let it swell for 24 hours to form a gel matrix. 2) Drug dispersion: Weigh 20 mg of Badu Shengji San and evenly disperse it in the above matrix using a homogenizer; 3) Add excipients: Add 50 mg of glycerin and 1 mg of methyl paraben (preservative) in sequence and stir until uniform; 4) pH adjustment: add triethanolamine to adjust the gel pH to 7.40; 5) Homogenization: Vortex mixer at 2000 rpm for 10 minutes to ensure uniform distribution of the drug.

[0050] Example 3 Stability Test 1. Centrifugation Test Take 1 g of gel sample and centrifuge at 2000 rpm for 30 minutes to observe whether there is any stratification, hydration or structural damage ( Figure 6 ).

[0051] 2. High temperature test The sample was placed in a 60°C oven for 24 hours. After returning to room temperature, there was no precipitation or stratification, and the spreadability and pH value did not change significantly ( Figure 7 ).

[0052] 3. Low temperature test The sample was frozen at -20℃ for 24 hours and after returning to room temperature, the gel morphology was intact without crystallization or phase separation ( Figure 8 ).

[0053] Example 4: Destructive Effect of the Toxin-Removing and Muscle-Regenerating Gel on Bacterial Biofilms 1. Crystal violet staining to measure the effect of Badu Shengji San (gel) on bacterial biofilm (1) Use sterile tweezers to pick up a Staphylococcus aureus colony on the blood agar plate with the pipette tip, place the pipette tip into a centrifuge tube containing 10 mL of TSB medium, and place the centrifuge tube containing the colony in a shaker for 18 h.

[0054] (2) Adjust the concentration of the bacterial solution until the value measured by the turbidimeter is 0.5 McFarland units, which means the concentration is 10 8 CFU / mL of bacterial solution, dilute the bacterial solution with TSB broth to a concentration of 10 6 CFU / mL and inoculate 100 μL into each well of a 96-well plate.

[0055] (3) After culturing in a 37°C biochemical incubator for 24 h, the samples were taken out and bacterial biofilm formation was observed.

[0056] (4) Weigh 51.2 mg of Badu Shengji San (gel) and dissolve it in 50 mL of TSB culture medium to prepare a 1024 μg / mL drug solution. Use the two-fold dilution method to dilute the solution to the following concentrations: 512 μg / mL, 256 μg / mL, 128 μg / mL, 64 μg / mL, 32 μg / mL, 16 μg / mL, 8 μg / mL, 4 μg / mL, 2 μg / mL, 1 μg / mL, and 0.5 μg / mL.

[0057] (5) Aspirate the bacterial suspension above the biofilm in each well and add 200 μL of the above-mentioned gradient drug solution to each well. The control wells are left untreated. Continue to incubate them in a 37°C biochemical incubator for 24 h.

[0058] (6) Take out the well plate and observe the biofilm structure in each well from low concentration to high concentration with the naked eye.

[0059] (7) Add 50 μL of 4% paraformaldehyde fixative to each well and fix for 10 min. Aspirate the fixative and add 200 μL of PBS to rinse gently and then aspirate.

[0060] (8) Add 100 μL of crystal violet staining solution to each well. After staining for 10 min, aspirate the staining solution and add 200 μL of PBS to each well.

[0061] (9) Rinse gently several times until the supernatant is colorless.

[0062] (10) Place the plate in a ventilated place to dry, and add 200 μL of 33% acetic acid solution to each well to dissolve crystal violet for about 5 minutes.

[0063] (11) Take another well plate and transfer 150 μL of the solution from each well of the original well plate to the corresponding well plate using a pipette. Measure the OD value of each well at λ = 595 nm in a microplate reader.

[0064] The test results are as follows Figure 9 As shown in the figure, it was found that the biofilm stained with crystal violet decreased successively as the drug concentration increased from 0.5 μg / mL to 512 μg / mL. At higher drug concentrations (>64 μg / mL), both the Badu Shengji San and Badu Shengji Gel groups had a certain degree of destructive effect on bacterial biofilm.

[0065] 2. Scanning electron microscopy observation of the effect of Badu Shengji San (gel) on bacterial biofilm (1) In a biosafety cabinet, place a sterilized 9 mm diameter coverslip into the bottom of a 24-well plate.

[0066] (2) Pipette 800 μL of a solution with a concentration of approximately 10 6 The Staphylococcus aureus liquid diluted with TSB medium was placed in the well plate and incubated at 37 °C in a biochemical incubator for 24 h.

[0067] (3) Remove the 24-well plate, discard the old TSB medium, and add 1 mL of 128 μg / mL and 64 μg / mL Badu Shengji San (gel) to each well. For the blank control, add 1 mL of TSB medium.

[0068] (4) After 24 hours, aspirate the solution in the well plate, add PBS to the well plate and wash the plate once.

[0069] (5) Add 1 mL of 2.5% glutaraldehyde to the wall of each well and place in a 4°C refrigerator for fixation for 24 h.

[0070] (6) Aspirate the fixative and rinse the sample three times with 0.1 M, pH 7.0 PBS, adhering to the plate, for 15 minutes each time.

[0071] (7) In a biosafety cabinet, fix the sample with 1% osmium hydroxide solution for 1 h.

[0072] (8) Carefully aspirate the osmium acid solution and rinse the sample three times with 0.1 M, pH 7.0 PBS, for 15 min each time.

[0073] (9) Dehydrate the samples with ethanol solutions of gradient concentrations (30%, 50%, 70%, 80%, 90% and 95%) for 15 min at each concentration, then treat with 100% ethanol for 20 min. Finally, replace with new 100% ethanol and store the samples in 100% ethanol.

[0074] (10) The coverslips were removed from the 24-well plate and dried in a Hitachi HCP-2 critical point dryer.

[0075] (11) The films were deposited in a Hitachi E-1010 ion plating instrument, and the treated samples were observed in a Hitachi SU-8010 scanning electron microscope.

[0076] The test results are as follows Figure 10As shown, in the blank control group, individual S. aureus bacteria exhibited small spherical structures, connected to other bacteria through the extracellular matrix secreted by the bacteria, forming aggregated grape-like structures. Irregular structures, representing extracellular matrix, were exposed at the edges of the spherules. In the bacterial biofilm treated with 128 μg / mL Badu Shengji Powder, a large number of S. aureus bacteria detached from the biofilm, exposing a complex, interwoven, filamentous extracellular matrix structure, as well as a small number of S. aureus bacteria that remained encapsulated within the extracellular matrix. In the 128 μg / mL Badu Shengji Powder group, surface S. aureus bacteria detached, but a large number of bacteria remained embedded within the extracellular matrix. The density of bacteria encapsulated within the extracellular matrix was greater in the 128 μg / mL Badu Shengji Powder group, indicating that Badu Shengji Powder had a less destructive effect on the bacterial biofilm than Badu Shengji Powder. As the drug concentration decreased, the complex and interwoven filamentous extracellular matrix structure in the photos of the 64 μg / mL Badu Shengji Gel and Badu Shengji Powder groups gradually transformed into a soil-buried structure, suggesting that the damage to the bacterial biofilm structure was further reduced, while the density of bacteria in the extracellular matrix also increased significantly, indicating that as the drug concentration decreased, Staphylococcus aureus reduced its shedding from the extracellular matrix.

[0077] Example 5 The therapeutic effect of the toxin-eliminating and tissue-promoting gel on burns Rats were anesthetized with an intraperitoneal injection of 2% sodium pentobarbital. The backs of the rats were physically shaved and then chemically depilated. A custom-made rectangular brass comb, approximately 20 mm × 10 mm, was boiled in 100°C water for 5 min. The weight of the comb was then used to scald the rats' backs for 25 s, resulting in characteristic lesions of deep second-degree burns, including epidermal thinning, epithelial cell nuclear elongation, dermal collagen changes, and inflammatory cell infiltration.

[0078] Apply 1mg / cm2 to the burned skin of rats 2 / time dose of the drug was given, while the blank gel group was given an equal amount of gel without Badu Shengji Powder. The wound was covered with 4 layers of vaseline oil sand as the inner dressing and 4 layers of gauze as the outer dressing. The elastic bandage was used to apply slight pressure and the dressing was changed every 2 days.

[0079] 1. Photography of rat burn wounds Photos were taken at 0h, 4D, 8D, 14D, and 21D after burn ( Figure 11At 0 hours, the deep second-degree burns created using a brass comb took on a comb-like appearance, demonstrating successful surface modeling. By day 4, necrosis began to appear on the burned skin of rats in the Badu Shengji Powder and blank control groups, while necrosis did not develop until day 8 in the Badu Shengji Gel and blank gel groups. This suggests that the gel formulation can, to a certain extent, delay skin tissue necrosis and skin cell apoptosis in the early stages of deep second-degree burn wounds. From day 8 to day 14, the necrosis at the burn site of the rats solidified into a dense scab, visibly blocking drug transdermal absorption. Trimming the scabs at the edges revealed abundant bleeding beneath the scabs in the Badu Shengji Gel group. By day 21, significant wound infection developed in the blank gel group, while infection occurred in a small portion of the wounds in the blank control group. The wound area in the Badu Shengji Gel group was significantly smaller than that in the other three groups, and no infection occurred.

[0080] 2. Hematoxylin and eosin (HE) staining H&E staining of paraffin sections of the skin from the four groups of rats at 0h revealed characteristic deep II degree lesions on the burn wound surface, with thinning of the epidermis, elongated epithelial cell nuclei, hyalinization of the dermis, and infiltration of inflammatory cells. No significant differences were found in the H&E staining images between 12h and 72h. Starting on the seventh day, the number of new blood vessels in the Badu Shengji Gel group was greater than that in the Badu Shengji Powder group, and the number of new blood vessels in the Badu Shengji Powder group was significantly greater than that in the blank gel and blank control groups ( Figure 12 ).

[0081] 3. Cytokeratin 10 Immunohistochemical Staining On 21 days, paraffin sections of the skin of the four groups of rats were taken for immunohistochemical staining, and cytokeratin 10 (as a marker of the epithelial basal layer and granular cell layer) was used. The results showed that the thickness of the new epidermis in the Badu Shengji Gel group and the Badu Shengji Powder group was significantly thicker than that in the blank gel group and the blank control group, and the thickness of the Badu Shengji Gel group was thicker than that in the Badu Shengji Powder group ( Figure 13 ).

[0082] 4. Modified Sirius Red Staining Paraffin sections of rat skin were taken at 3, 7, 14, and 21 days and stained with modified Sirius red to characterize the effects of Badu Shengji Gel and powder on deep grade II wounds. Type I collagen is a relatively large type of collagen synthesized by adult dermal fibroblasts, which supports skin hardness and firmness. Type III collagen is the main component of reticular fibers in the skin. During wound repair, the proportion of type III collagen increases. The area ratio of type I and type III collagen fibers in the Badu Shengji Gel and Badu Shengji Powder groups was significantly different from that in the blank gel and control groups ( Figure 14 ).

Claims

1. A gel for removing toxins and promoting tissue regeneration, characterized in that: Includes the following ingredients and their mass percentages: Badu Shengji San: 0.5% to 2%; Gel matrix: 1% to 5%; Moisturizer: 2% to 10%; pH regulator to maintain the pH value of the gel at 6.0 to 7.4; The balance is water, The gel matrix is Carbomer 940.

2. The gel for removing toxins and promoting tissue regeneration as claimed in claim 1, wherein: The moisturizing agent is glycerin.

3. The toxin-eliminating and granulation-promoting gel according to claim 1, wherein: The pH regulator is triethanolamine.

4. The toxin-eliminating and granulation-promoting gel according to any one of claims 1 to 3, wherein: The mass percentage of the gel matrix is 5%, the mass percentage of the Badu Shengji San is 2%, the mass percentage of the moisturizer is 5%, and the pH value of the gel is 7.

4.

5. The toxin-eliminating and granulation-promoting gel according to claim 1, wherein The invention also includes a preservative, which is methyl parahydroxybenzoate, and the mass percentage of the preservative is 0.1% to 0.5%.

6. The preparation method of the toxin-eliminating and granulation-promoting gel according to claim 1, wherein The following steps are involved: 1) Disperse Carbomer 940 in water and allow it to swell to form a gel matrix; 2) Evenly disperse the Badu Shengji Powder in the gel matrix prepared in step 1); 3) Add glycerin and stir until uniform; 4) Adjust the pH value of the gel to 6.0-7.4; 5) The drug is evenly distributed through homogenization.

7. The preparation method according to claim 6, wherein The swelling time of Carbomer 940 in step 1) is 24 hours.

8. The preparation method according to claim 6, wherein The homogenization treatment in step 5) was performed using a vortex mixer at a rotation speed of 2000 rpm for 10 minutes.

9. Use of the toxin-eliminating and tissue-promoting gel of claim 1 in the preparation of a medicament for treating chronic ulcerative diseases, wherein the chronic ulcerative diseases include burns and scalds.

10. The use according to claim 9, characterized in that: The toxin-removing and tissue-regenerating gel can repair burn and scald wounds by inhibiting bacterial biofilm formation and promoting collagen synthesis.

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