Culture medium for promoting growth of edible mushroom mycelia and preparation method and application thereof

Abstract

The invention belongs to the technical field of edible mushroom cultivation, and discloses a culture medium for promoting growth of edible mushroom hyphae and a preparation method and application of the culture medium. The culture medium comprises the following raw materials in parts by weight: 50-90 parts of cottonseed hulls, 10-30 parts of bran, 3-15 parts of corn flour, 2-10 parts of ferula asafetida root powder, 1-3 parts of ginkgo biloba extract, 4-8 parts of humic acid, 3-5 parts of perlite, 1-3 parts of sodium alginate, 1-3 parts of lime and 0.5-2 parts of chitosan. The raw materials are reasonable in compatibility, the obtained culture medium can remarkably promote rapid growth of edible mushroom hyphae and enhance stress resistance, the hyphae are dense and high in survival rate, and infectious microbes are avoided. The ferula asafetida root powder and the ginkgo leaf extract have a synergistic effect, so that the absorption and utilization efficiency of the hyphae on nutrient substances is further enhanced, and the ferula asafetida root powder and the ginkgo leaf extract jointly promote rapid growth and branching of the hyphae.

Description

Technical Field

[0001] The invention belongs to the technical field of edible fungus cultivation, and more particularly relates to a culture medium for promoting the growth of edible fungus mycelium, and a preparation method and application thereof. Background Art

[0002] Among known edible fungi, various developmental challenges exist. Only about 40 known species can be mass-produced, and many rare wild mushrooms, such as Ganba mushrooms, are difficult to cultivate artificially. Even when successfully cultivated, they often suffer from a lack of flavor. This is primarily due to the fact that edible fungi are mostly saprophytic or parasitic, living in symbiosis with tree roots. For example, the Xinjiang ferula mushrooms are saprophytic or parasitic on the roots of ferula, the Xinjiang willow mushrooms grow in willow trunks, and the Ganba mushrooms grow in the pine needles beneath pine trees. These complex growing environments present significant challenges for those involved in the edible fungus industry. Cultivating edible fungus stock is a critical step in production, and the performance of the culture medium directly impacts the growth rate, robustness, and stress resistance of the mycelium. Traditional culture media, typically consisting of basic ingredients such as cottonseed hulls and bran, can only meet the basic growth requirements of the mycelium, but lack synergistic benefits among the ingredients, resulting in inadequate growth and enhanced stress resistance.

[0003] Asafoetida, also known as stinking asafoetida, is an herbaceous plant of the genus Ferula in the Apiaceae family. The plant is about 1 meter tall and has a pungent odor. This plant is mainly distributed in Russia and Xinjiang, my country, and grows on salinized grasslands. There are few studies on artificial introduction and cultivation methods. Asafoetida root is a traditional Chinese medicine with a unique odor obtained by air-drying asafoetida. It has therapeutic effects on meat stagnation, abdominal lumps, etc., and is often used to treat cold pain in the abdomen, food stagnation, worm accumulation and other diseases. Asafoetida mushroom, also known as Pleurotus ferulae, is an edible fungus belonging to the Agaricales order and the Agaricus family. Asafoetida mushroom is a cultivable edible fungus that is loved by people for its unique flavor and nutritional value comparable to that of "porcini". Asafoetida mushroom parasitizes on the rhizomes of Asafoetida in spring, growing alone or nearly in clusters. At present, there is no research on combining asafoetida root with other raw materials to affect the growth of mycelium of different edible fungi.

[0004] Therefore, how to obtain a culture medium that promotes the growth of mycelia of different edible fungi is a technical problem that those skilled in the art urgently need to solve. Summary of the Invention

[0005] In order to overcome the shortcomings and deficiencies in the prior art, the present invention provides a culture medium for promoting the growth of edible fungus mycelium, and a preparation method and application thereof.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A culture medium for promoting the growth of edible fungus mycelium comprises the following raw materials, measured by weight: 50-90 parts of cottonseed hulls, 10-30 parts of bran, 3-15 parts of corn flour, 2-10 parts of ferula root powder, 1-3 parts of ginkgo leaf extract, 4-8 parts of humic acid, 3-5 parts of perlite, 1-3 parts of sodium alginate, 1-3 parts of lime, and 0.5-2 parts of chitosan.

[0008] As the same inventive concept as the above technical solution, the present invention also claims protection for a method for preparing the above culture medium, comprising the following steps:

[0009] (1) Washing, drying, and crushing ginkgo leaves into powder, adding ethanol solution, and performing reflux extraction 1 to 3 times, combining the extracts, centrifuging, and concentrating the obtained supernatant under reduced pressure, and freeze-drying to obtain ginkgo leaf extract;

[0010] (2) pre-treating the cottonseed hulls and passing them through a 20-mesh sieve to remove impurities;

[0011] (3) The pretreated cottonseed hulls, bran, and corn flour are put into a three-dimensional mixer and mixed, and then the root powder of Ferula foetida, Ginkgo biloba extract, humic acid, perlite, sodium alginate, lime, and chitosan are added in sequence, the speed is increased, and the mixture is mixed again;

[0012] (4) Continue to add deionized water and perform microwave sterilization to obtain culture medium.

[0013] Preferably, the mass volume ratio of the powder and the ethanol solution in step (1) is 1:8-15 g / mL, the volume fraction of the ethanol solution is 60-70%, the temperature of the reflux extraction is 60-70° C., and the time for each extraction is 1 to 2 hours.

[0014] Preferably, the temperature of the reduced pressure concentration in step (1) is 50 to 60°C; the temperature of the freeze-drying is -60 to -40°C, and the pressure is 90 to 100 Pa.

[0015] Preferably, the mixing speed in step (3) is 40-50 rpm and the mixing time is 10-15 min.

[0016] Preferably, in step (3), the rotation speed is increased to 80-100 rpm, and the mixing time is again 20-30 min.

[0017] Preferably, the mass ratio of the deionized water to the total amount of the raw materials in step (4) is 1.2-1.5:1.

[0018] Preferably, the frequency of the microwave sterilization in step (4) is 915 MHz.

[0019] As an inventive concept identical to the above technical solution, the present invention also seeks to protect the use of the above culture medium in cultivating edible fungi.

[0020] Preferably, the edible fungi include any one of Pleurotus eryngii, Pleurotus ostreatus, Coprinus comatus, Pleurotus ostreatus, and Pleurotus ferulae.

[0021] It can be seen from the above technical solutions that, compared with the prior art, the present invention provides a culture medium for promoting the growth of edible fungi mycelium, and a preparation method and application thereof, which have the following beneficial effects:

[0022] 1. The raw material compatibility of the present invention is reasonable, and the obtained culture medium can significantly promote the rapid growth of edible fungi mycelia and enhance stress resistance. The mycelia are dense and have a high survival rate, which avoids the generation of miscellaneous bacteria. The flavonoids in the ginkgo leaf extract can regulate the hormone balance in the mycelial cells, promote the synthesis of cytokinins, and simultaneously activate the activity of related enzymes, thereby accelerating the growth metabolism of mycelia. The active ingredients such as terpenoids in the root of Ferula foetida powder and the flavonoids act synergistically to further enhance the absorption and utilization efficiency of mycelia to nutrients, and the two jointly promote the rapid growth and branching of mycelia. At the same time, the ginkgo leaf extract has a strong antioxidant capacity, can remove the free radicals produced by mycelia during growth, protect the integrity of cell structure and biofilm, and improve the stress resistance of mycelia. The components such as alkaloids in the root of Ferula foetida powder can enhance the strength and toughness of mycelial cell walls, and the two jointly improve stress resistance.

[0023] 2. The chitosan of the present invention, as a cationic antimicrobial agent, forms a positively and negatively charged adsorption layer with the alkaloids in the root powder of Ferula foetida, disrupting the integrity of bacterial cell membranes. The two synergistically enhance the antimicrobial effect and reduce the rate of bacterial contamination. Perlite provides a porous and breathable structure, which, together with sodium alginate and humic acid, creates a physical structure that enhances the oxygen diffusion and moisture retention capacity of the culture medium, creating a favorable physical environment for mycelial growth and promoting mycelial colonization and expansion.

[0024] 3. The culture medium of the present invention uses microwave sterilization to retain more active ingredients in the ginkgo leaf extract. The porous structure of perlite can effectively disperse heat during the sterilization process, preventing local high temperature points from damaging the extract. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are merely embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying any creative work.

[0026] Figure 1These are the results of the mushroom fruiting experiment in Experiment 5 in which the group with added Ferula root powder was added.

[0027] Figure 2 These are the fruiting results of the control group in Experiment 5. DETAILED DESCRIPTION

[0028] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of them. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0029] Example 1

[0030] A culture medium for promoting the growth of edible fungus mycelia comprises the following raw materials, measured by weight: 80 parts of cottonseed hulls, 15 parts of bran, 5 parts of corn flour, 8 parts of asafoetida root powder, 2 parts of ginkgo leaf extract, 5 parts of humic acid, 5 parts of perlite, 2 parts of sodium alginate, 2 parts of lime, and 1 part of chitosan.

[0031] The method for preparing the above-mentioned culture medium comprises the following steps:

[0032] (1) Washing, drying, and crushing ginkgo leaves into powder, adding ethanol solution, and performing reflux extraction twice, combining the extracts, centrifuging, and concentrating the obtained supernatant under reduced pressure, and freeze-drying to obtain ginkgo leaf extract;

[0033] (2) pre-treating the cottonseed hulls and passing them through a 20-mesh sieve to remove impurities;

[0034] (3) The pretreated cottonseed hulls, bran, and corn flour were put into a three-dimensional mixer and mixed evenly. Then, ginkgo leaf extract, humic acid, perlite, sodium alginate, lime, and chitosan were added in sequence, the speed was increased, and the mixture was mixed evenly again.

[0035] (4) Continue to add deionized water and perform microwave sterilization to obtain culture medium.

[0036] In step (1), the mass volume ratio of the powder to the ethanol solution is 1:10 g / mL, the volume fraction of the ethanol solution is 70%, the temperature of the reflux extraction is 70°C, and the time of each extraction is 2 hours. The temperature of the reduced pressure concentration is 60°C; the temperature of the freeze drying is -60°C, and the pressure is 100 Pa.

[0037] In step (3), the mixing speed is 50 rpm and the mixing time is 10 min. The speed is increased to 100 rpm and the mixing time is again 30 min.

[0038] The mass ratio of the deionized water to the total amount of the raw materials in step (4) is 1.3: 1. The frequency of the microwave sterilization is 915 MHz.

[0039] Experiment 1

[0040] According to the method of Example 1, the effects of different dosages of Ferula root powder on the growth of Pleurotus ostreatus mycelium were detected.

[0041] The specific steps are as follows:

[0042] 1.1 Preparation of stock culture medium

[0043] Six groups were established, each differing in the addition of varying amounts of Ferula root powder. A control (CK) was used without Ferula root powder. The materials were weighed according to the recipe, piled for 2 hours to achieve uniform moisture, and then bottled. Each group consisted of 10 culture bottles. After filling, the bottles were numbered in batches and sterilized before being transferred to a clean bench in the inoculation room.

[0044] 1.2 Mycelium culture and observation

[0045] After washing hands, spray them evenly with alcohol. Then, evenly spray the bottles of various mushroom cultures used in the experiment with alcohol and transfer them to the clean bench for inoculation. Once inoculation is complete, place the inoculated bottles in the incubation room. During the incubation period, measure the mycelium every four days until growth stabilizes and observe its density.

[0046] 1.3 Primordial Observation

[0047] After the mycelium is cultured to full size in the bottle, observe the growth and quantity of the primordia.

[0048] 1.4 Data Processing

[0049] The mycelial growth data were processed using a single-factor ANOVA. The results are shown in Table 1.

[0050] Table 1 Effect of Ferula root powder on the growth of Pleurotus ostreatus mycelium

[0051] Root of Asafoetida powder parts by weight Mycelial growth rate cm / d Whiteness Full bottle formation time Number of primordia 0 0.57±0.03c +++ 37d A cluster, very sparse 2 0.65±0.08bc +++ 32d A cluster, sparse 4 0.69±0.03abc +++ 30d A cluster, sparse 6 0.70±0.04abc ++++ 28d Two clusters, very thriving 8 0.74±0.02ab ++++ 25d Two clusters, more vigorous 10 0.76±0.02a ++++ 24d Two clusters, more vigorous

[0052] Note: "+" indicates sparse mycelia with weak growth, "++" indicates dense mycelia with strong growth, "+++" indicates dense mycelia with strong growth, and "++++" indicates very dense mycelia with very strong growth. The values ​​in the table are the average values ​​of the measurements, the same below.

[0053] As can be seen from Table 1, in terms of mycelial growth rate, within the experimental addition range (0-10%), there was a significant promoting effect, and no inhibition was observed, with the 10% treatment having the strongest promoting effect. In terms of primordium formation time and primordium quantity, the addition of ferula root powder had a good promoting effect on both primordium formation time and primordium quantity, with the 6% treatment showing the most vigorous primordium growth. Pleurotus ostreatus has a strong adaptability to sawdust-based cultivation materials and can utilize a wide range of sawdust. The active substances in ferula root powder also promote the growth of pleurotus ostreatus. Therefore, it is shown that within a certain range, the promotion effect is significant with the addition of ferula root powder.

[0054] Experiment 2

[0055] The same method as in Experiment 1 was used to test the effects of different dosages of Ferula root powder on the mycelial growth of Pleurotus eryngii. The results are shown in Table 2.

[0056] Table 2 Effects of Ferula root powder on the mycelial growth of Pleurotus eryngii

[0057] Root of Asafoetida powder parts by weight Mycelial growth rate cm / d Whiteness Full bottle time 0 0.31±0.02b ++ 50d 2 0.40±0.03ab ++ 42d 4 0.47±0.02ab +++ 40d 6 0.51±0.06ab +++ 35d 8 0.52±0.05a +++ 34d 10 0.54±0.02a +++ 31d

[0058] Table 2 shows that mycelial growth rate was promoted within the experimental addition range (0-10%), with no inhibition observed. The growth rate and bottle-fill time showed the same trend. The significance trend showed a significant promoting effect within the experimental range. King Oyster Mushroom belongs to the larger family of Oyster Mushrooms and shares similar growth characteristics with them, demonstrating good adaptability to sawdust. The active substances in Ferula root powder also promote the growth of King Oyster Mushroom.

[0059] Experiment 3

[0060] The same method as in Experiment 1 was used to test the effects of different dosages of Ferula root powder on the mycelial growth of Agaricus terrestris. The results are shown in Table 3.

[0061] Table 3 Effect of Ferula root powder on the mycelial growth of Agaricus terrestris

[0062] Root of Asafoetida powder parts by weight Mycelium growth rate cm / d Whiteness Full bottle time 0 0.52±0.04b ++++ 37d 2 0.61±0.02ab ++++ 29d 4 0.68±0.07a ++++ 26d 6 0.63±0.02ab ++++ 28d 8 0.60±0.03ab ++++ 29d 10 0.58±0.02ab ++++ 32d

[0063] Table 3 shows that, in terms of average growth rate, within the experimental addition range (0-10%), the overall trend showed a promoting effect, with no significant inhibitory effect observed. The 4% treatment showed the fastest growth rate, demonstrating a significant promoting effect. The growth rate and time to full bottle showed a similar trend. Overall, a promoting effect was observed within the 2%-10% addition range, with the 4% treatment being the most effective.

[0064] Experiment 4

[0065] The same method as in Experiment 1 was used to test the effects of different dosages of Ferula root powder on the mycelial growth of Coprinus comatus. The results are shown in Table 4.

[0066] Table 4 Effects of Ferula root powder on mycelial growth of Coprinus comatus

[0067] Root of Asafoetida powder parts by weight Mycelial growth rate cm / d Whiteness Full bottle time 0 0.62±0.02b + 31d 2 0.69±0.04ab + 28d 4 0.75±0.02ab ++ 23d 6 0.77±0.04ab ++ 22d 8 0.81±0.02a ++ 20d 10 0.71±0.04ab ++ 26d

[0068] As can be seen from Table 4, from the perspective of average growth rate, within the experimental addition range (0-10%), the overall trend showed a promoting trend, and no obvious inhibitory effect was observed. Among them, the growth rate of the 8% treatment was the fastest, and the promoting effect was obvious; from the full bottle time, it can be seen that the growth rate and the full bottle time trend are basically the same. Overall, there is a promoting effect in the range of 2%-10%, and the 8% treatment is the best. Pheasant leg mushroom has a strong ability to utilize cellulose and lignin. In this experiment, the addition of ferula root powder acted as sawdust. As the addition amount increased, the promoting effect became more obvious.

[0069] Experiment 5

[0070] The same method as in Experiment 1 was used to test the effects of different dosages of Ferula root powder on the mycelial growth of Pleurotus ferulae. The results are shown in Table 5.

[0071] Table 5 Effect of Ferula root powder on the mycelial growth of Pleurotus ferulae

[0072] Root of Asafoetida powder parts by weight Mycelial growth rate cm / d Whiteness Full bottle time 0 0.38±0.03b ++++ 52d 2 0.43±0.01ab ++++ 48d 4 0.44±0.03ab ++++ 46d 6 0.48±0.02ab ++++ 40d 8 0.51±0.06a ++++ 37d 10 0.47±0.03ab ++++ 42d

[0073] As can be seen from Table 5, in terms of average growth rate, within the experimental addition range (0-10%), the overall trend showed a promoting effect, with no significant inhibitory effect observed. Among them, the 8% treatment had the fastest growth rate, showing a significant promoting effect; as can be seen from the bottle-full time, the growth rate and bottle-full time trends were essentially the same. The root of Ferula contains trace substances that promote the growth of Ferula mushrooms, helping them to play a role in mycelial growth, primordium formation, inhibiting mushroom flies and maggots, and improving fruiting body quality. Overall, a promoting effect was observed within the 2%-10% range, with the 8% treatment being the best.

[0074] Carry out mushroom fruiting experiment: the formula of cultivation material is the same as the formula of original seed culture medium in Example 1, and is divided into a group with added root powder of Asafoetida and a control group. The only difference is whether the root powder of Asafoetida is added. Each group has 30 bags, and a total of 60 treatments. The fungus bags are moved into a constant temperature culture room and cultured in a dark condition with a temperature of 20-25°C and a humidity of 60%. After the bags are full, they are moved to the fruiting room for fruiting. The number of mushrooms produced is determined by observing the number of fungus bags with added root powder of Asafoetida and the control group at the same time. When carrying out the fruiting test, the ratio of the number of fruiting fungus bags to the total number of fungus bags in the same period of time is the fruiting rate. The results are shown in Table 6 and Figure 1 、 Figure 2 shown.

[0075] Table 6 Mushroom fruiting experimental results

[0076]

[0077]

[0078] From Table 6 and Figure 1 、 Figure 2 It can be seen that the addition of ferula root powder has a promoting effect on Pleurotus ferulae. During the recording process of the fruiting experiment on Pleurotus ferulae, the group with ferula root powder added had earlier primordium formation and larger fruiting bodies than the control group.

[0079] Example 2

[0080] A culture medium for promoting the growth of edible fungus mycelia comprises the following raw materials, measured by weight: 50 parts of cotton seeds, 10 parts of bran, 3 parts of corn flour, 2 parts of asafoetida root powder, 1 part of ginkgo leaf extract, 4 parts of humic acid, 3 parts of perlite, 1 part of sodium alginate, 1 part of lime, and 0.5 part of chitosan.

[0081] The preparation method is the same as that of Example 1.

[0082] Example 3

[0083] A culture medium for promoting the growth of edible fungus mycelia comprises the following raw materials, measured by weight: 90 parts of cottonseed hulls, 30 parts of bran, 15 parts of corn flour, 10 parts of asafoetida root powder, 3 parts of ginkgo leaf extract, 8 parts of humic acid, 5 parts of perlite, 3 parts of sodium alginate, 3 parts of lime, and 2 parts of chitosan.

[0084] The preparation method is the same as that of Example 1.

[0085] Comparative Example 1

[0086] A culture medium, which differs from Example 1 in that no Ferula root powder is added.

[0087] Comparative Example 2

[0088] A culture medium, which differs from Example 1 in that no ginkgo leaf extract is added.

[0089] Comparative Example 3

[0090] A culture medium, which is different from that of Example 1 in that the weight portion of the asafoetida root powder is 20 parts.

[0091] Comparative Example 4

[0092] A culture medium, which differs from Example 1 in that, in step (4) of the preparation method, high-pressure sterilization is performed at 121° C. and 0.015 MPa for 2.5 h, and microwave sterilization is not performed.

[0093] A culture medium was prepared from each example and comparative example. The effect of the culture medium on the growth of Pleurotus ostreatus mycelium was studied using the method of Experiment 1. During the culture process, the culture medium was treated at a low temperature of 4°C for 7 days and at a high temperature of 30°C for 24 hours. The results are shown in Table 7.

[0094] Table 7

[0095]

[0096] Analysis shows that the root of Ferula powder of the present invention and Ginkgo biloba extract are indispensable, and the two have promoted mycelial growth and stress resistance in a coordinated manner, can cope with different harsh environments, and have a high survival rate. After the lack of key components, the comparative example has a significantly reduced effect. And when the root of Ferula powder is used in too much amount, mycelial growth can be suppressed, which is unfavorable for mycelial survival. Microwave sterilization of the present invention can retain the extract activity to the greatest extent, and comparative example 4 carries out high temperature and high pressure sterilization, destroys some active substances of Ginkgo biloba extract, and the flavonoid content is reduced, which weakens the synergistic effect with the root of Ferula powder.

[0097] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A culture medium for promoting the growth of edible fungi mycelium, characterized in that: The invention comprises the following raw materials in parts by weight: 50-90 parts of cottonseed hulls, 10-30 parts of bran, 3-15 parts of corn flour, 2-10 parts of asafoetida root powder, 1-3 parts of ginkgo leaf extract, 4-8 parts of humic acid, 3-5 parts of perlite, 1-3 parts of sodium alginate, 1-3 parts of lime and 0.5-2 parts of chitosan.

2. The method for preparing a culture medium according to claim 1, wherein The following steps are involved: (1) Washing, drying, and crushing ginkgo leaves into powder, adding ethanol solution, and performing reflux extraction 1 to 3 times, combining the extracts, centrifuging, and concentrating the obtained supernatant under reduced pressure, and freeze-drying to obtain ginkgo leaf extract; (2) pre-treating the cottonseed hulls and passing them through a 20-mesh sieve; (3) Mix the pretreated cottonseed hulls, bran, and corn flour, then add ferula root powder, ginkgo leaf extract, humic acid, perlite, sodium alginate, lime, and chitosan in sequence, and mix again; (4) Continue to add deionized water and sterilize to obtain the culture medium.

3. The method for preparing the culture medium according to claim 2, wherein The mass volume ratio of the powder and the ethanol solution in step (1) is 1:8-15 g / mL, the volume fraction of the ethanol solution is 60-70%, the temperature of the reflux extraction is 60-70° C., and the time of each extraction is 1 to 2 hours.

4. The method for preparing the culture medium according to claim 2, wherein The temperature of the reduced pressure concentration in step (1) is 50 to 60° C.; the temperature of the freeze drying is -60 to -40° C., and the pressure is 90 to 100 Pa.

5. The method for preparing the culture medium according to claim 2, wherein During the mixing in step (3), stirring is performed at a speed of 40-50 rpm for 10 to 15 minutes.

6. The method for preparing the culture medium according to claim 2, wherein In step (3), when the mixture is mixed evenly again, stirring is performed at a rotation speed of 80-100 rpm and a mixing time of 20 to 30 minutes.

7. The method for preparing a culture medium according to claim 2, wherein The mass ratio of the deionized water to the total amount of the raw materials in step (4) is 1.2-1.5:

1.

8. The method for preparing a culture medium according to claim 2, wherein The sterilization in step (4) is microwave sterilization, and the frequency of the microwave sterilization is 915 MHz.

9. Use of the culture medium according to claim 1 in cultivating edible fungi.

10. Use of the culture medium according to claim 9 in cultivating edible fungi, characterized in that: The edible fungi include any one of Pleurotus eryngii, Pleurotus ostreatus, Coprinus comatus, Pleurotus ostreatus and Pleurotus ferulae.

Images