Paenibacillus mucilaginosus and its application in synthesis of manganese oxide

Abstract

The present application provides a bacillus mycoides and its application in synthesizing manganese oxide, and the bacillus mycoides (Bacillus mycoides) CP203 with a preservation number of CGMCC No. 24208 is provided. Bacillus mycoides The present application further provides the application of the aforementioned bacillus mycoides in biosynthesis of manganese oxide, which can oxidize divalent manganese ions in a solution into manganese oxide without contact, the synthesized manganese oxide product does not contain organisms such as bacteria and spores, and the divalent manganese ions and other harmful substances in raw materials will not adversely affect the growth of microorganisms, which can significantly reduce the purification cost of biosynthesis of manganese oxide, expand the types and range of biologically treated manganese ion wastewater, and therefore the present application has important applications in the fields of biosynthesis of manganese oxide and hazardous waste water treatment.

Description

Technical Field

[0001] This invention relates to the field of biosynthesis of manganese oxide, and more particularly to a fungal Bacillus and its application in the synthesis of manganese oxide. Background Technology

[0002] Manganese is widely distributed in nature, ranking tenth among the elements in the Earth's crust and second among metallic elements. Manganese oxides are widely used for the removal of heavy metals and organic pollutants from water bodies due to their strong adsorption, catalytic, and oxidation capabilities.

[0003] In the fields of pollutant removal and ecological restoration, bio-manganese oxide derived from microorganisms is gradually attracting attention as a novel nanomaterial. Microorganisms capable of synthesizing bio-manganese oxide include bacteria and fungi. Compared to traditional chemical manganese oxide, bio-manganese oxide has a larger specific surface area and stronger adsorption, catalytic, and oxidation activities, playing a crucial role in the remediation of environmental pollution caused by heavy metals and organic matter.

[0004] Current technologies primarily employ a mixed culture method of microorganisms and divalent manganese ions (MnCl2 or MnSO4) to treat wastewater containing divalent manganese ions or to synthesize manganese oxide. This is known as the direct contact method, which involves directly introducing manganese-oxidizing microbial agents into water containing divalent manganese to remove divalent manganese ions, or immobilizing manganese-oxidizing microorganisms to synthesize manganese oxide through contact with water containing divalent manganese ions, which is then used for the adsorption of heavy metal ions or the oxidation of organic matter.

[0005] The problem with the aforementioned direct contact method is that the synthesized manganese oxide, whether in suspension or powder form, is a mixture of manganese oxide and organisms, making it impossible to obtain manganese oxide without organisms. Furthermore, high concentrations of manganese are detrimental to microbial growth, making it impossible to treat solutions with high manganese ion concentrations or solutions containing other toxic or harmful substances that affect microbial growth. Summary of the Invention

[0006] This invention provides a Bacillus fungi and its application in the synthesis of manganese oxide, which can synthesize manganese oxide from divalent manganese ions without contact. The product does not contain biological components, and the microorganisms in the synthesis process are not affected by the concentration of manganese ions or harmful substances.

[0007] In a first aspect, the present invention provides a Bacillus mycoides ( Bacillus mycoides CP203, the Bacillus mycoides ( Bacillus mycoides CP203 was deposited on December 28, 2021, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 24208, and is classified as follows: Bacillus mycoidesThe depositary address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing, China.

[0008] The Bacillus mycoides provided by this invention ( Bacillus mycoides CP203 can synthesize manganese oxide without biological organisms in a non-contact manner. Its product has a wider range of applications than manganese oxide synthesized by existing technologies, and it has significant advantages, especially in application scenarios where it is not suitable to introduce biological organisms.

[0009] Meanwhile, the non-contact method for synthesizing manganese oxide can disregard the tolerance of bacteria to manganese ions and can also process manganese ion solutions containing other metal ions or organic matter, depending on the application scenario.

[0010] Secondly, the present invention provides a microbial inoculant comprising Bacillus mycoides (B. mycoides). Bacillus mycoides CP203 or its freeze-dried cells, or its bacterial suspension, or the supernatant after centrifugation of the bacterial suspension, or the precipitate resuspension after centrifugation of the bacterial suspension;

[0011] The microbial agent Bacillus mycoides ( Bacillus mycoides CP203 viable bacteria content ≥1×10 8 cfu / mL.

[0012] Thirdly, the present invention provides a product comprising Bacillus mycoides (… Bacillus mycoides CP203 or its freeze-dried cells, or its bacterial suspension, or the supernatant after centrifugation of the bacterial suspension, or the precipitate resuspension after centrifugation of the bacterial suspension, or microbial agents;

[0013] The product is Bacillus mycoides ( Bacillus mycoides CP203 viable bacteria content ≥1×10 8 cfu / mL;

[0014] The product is a biocatalyst or a wastewater treatment agent.

[0015] Fourthly, the present invention provides the above-mentioned Bacillus mycoides ( Bacillus mycoides The application of CP203 or its freeze-dried cells, or its bacterial suspension, or the supernatant after centrifugation of the bacterial suspension, or the precipitate resuspension after centrifugation of the bacterial suspension, or the above-mentioned microbial agents or the above-mentioned products in the biosynthesis of manganese oxide.

[0016] The present invention further provides the above-mentioned Bacillus mycoides ( Bacillus mycoides The application of CP203 or its freeze-dried cells, or its bacterial solution, or the supernatant after centrifugation of the bacterial solution, or the precipitate resuspension after centrifugation of the bacterial solution, or the above-mentioned microbial agents or products in the treatment of wastewater containing divalent manganese ions.

[0017] Fifthly, the present invention provides a method for synthesizing manganese oxide, using the aforementioned Bacillus mycoides (… Bacillus mycoides Manganese oxide is synthesized from a solution containing divalent manganese ions using CP203 or its freeze-dried cells, its bacterial solution, the supernatant after centrifugation of the bacterial solution, the precipitate resuspension after centrifugation of the bacterial solution, or the above-mentioned microbial agents or the above-mentioned products in a contact or non-contact manner.

[0018] Preferably, in the method described above, the method for contact synthesis of manganese oxide is as follows: [The text abruptly ends here, likely due to an incomplete sentence or missing information.] Bacillus mycoides CP203 or its freeze-dried cells, or its bacterial solution, or the supernatant after centrifugation of the bacterial solution, or the precipitate resuspension after centrifugation of the bacterial solution, or the above-mentioned microbial agents or the above-mentioned products are added to a solution containing divalent manganese ions.

[0019] Preferably, in the above method, the non-contact synthesis of manganese oxide is performed by: taking the above-mentioned Bacillus mycoides (… Bacillus mycoides CP203 or its freeze-dried cells, or its bacterial suspension, or the supernatant after centrifugation of the bacterial suspension, or the precipitate resuspension after centrifugation of the bacterial suspension, or the above-mentioned microbial agents, or the above-mentioned products, and solutions containing divalent manganese ions are each open and not sealed, and are in the same space.

[0020] The temperature in the space is 25-30℃, and the non-contact synthesis of manganese oxide is placed for 3-10 days.

[0021] Through the above method, the Bacillus mycoides (Bacillus) provided by the present invention Bacillus mycoides CP203 can oxidize manganese ions to manganese oxide without contact with the divalent manganese ion solution. The bacterial culture medium is completely separated from the manganese oxide solution, which will not cause biological contamination of manganese oxide, and also avoid the adverse effects of substances present in the divalent manganese ion solution on bacterial growth.

[0022] More preferably, in the above method, the concentration of manganese ions in the solution containing divalent manganese ions is 1.0-20.0 mmol / L, preferably 5.0-20.0 mmol / L.

[0023] Sixthly, the present invention provides a method for treating divalent manganese ions in wastewater, wherein the above-mentioned Bacillus mycoides ( Bacillus mycoides CP203 or its freeze-dried cells, or its bacterial solution, or the supernatant after centrifugation of the bacterial solution, or the precipitate resuspension after centrifugation of the bacterial solution, or the above-mentioned microbial agents or the above-mentioned products, and wastewater containing divalent manganese ions are each open and not sealed, and are in the same space.

[0024] The temperature in the space is 25-30℃, and the non-contact oxidation culture time is 3-10 days.

[0025] Preferably, in the above method, the source of divalent manganese ions in the solution or wastewater is MnCl2 or MnSO4.

[0026] The technical effects achieved by this invention are as follows:

[0027] This invention isolated a strain of Bacillus mycoides from the soil of a former manganese mine site. Bacillus mycoides CP203, with accession number CGMCC No. 24208. This strain can oxidize divalent manganese ions in solution to manganese oxide without contact. The manganese oxide product synthesized through this non-contact method does not contain bacterial cells, spores, or other organisms, and the divalent manganese ions and other potentially harmful substances in the raw materials will not adversely affect microbial growth. The *Bacillus mycosisvirus* strain of this invention (…) Bacillus mycoides The non-contact synthesis of manganese oxide using CP203 is a novel, efficient, flexible, and green method for synthesizing manganese oxide. It is of great significance for improving the removal efficiency of divalent manganese ions, flexibly preparing manganese oxide, and expanding the application scenarios of manganese oxide. Attached Figure Description

[0028] To more clearly illustrate the technical solutions in this invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0029] Figure 1 This invention is a phylogenetic tree constructed based on the 16S rRNA gene of Bacillus mycoides CP203.

[0030] Figure 2 This is a graph showing the yield of manganese oxide synthesized by direct contact of Bacillus mycoides CP203 at different Mn(II) concentrations.

[0031] Figure 3 This is an experimental result of Bacillus mycoides CP203 synthesizing manganese oxide via a non-contact method.

[0032] Figure 4 This is a graph showing the yield of manganese oxide synthesized by Bacillus mycoides CP203 in a non-contact manner at different Mn(II) concentrations.

[0033] Figure 5 These are scanning electron microscope results, among which... Figure 5 A is a pure strain of Bacillus mycoides CP203. Figure 5 B is a mixture of Bacillus mycoides CP203 and manganese oxide products synthesized through direct contact. Figure 5C is a manganese oxide product synthesized by Bacillus mycoides CP203 via a non-contact process.

[0034] Figure 6 The results are transmission electron microscopy and elemental analysis of manganese oxide synthesized by Bacillus mycosis fungoides CP203 via a non-contact method.

[0035] Figure 7 The effect of Cd(II) on the yield of manganese oxide synthesized by Bacillus mycosis fungi CP203 via a non-contact method. Detailed Implementation

[0036] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0037] Example 1: Screening, isolation, and identification of CP203

[0038] Bacillus mycosisviridae ( Bacillus mycoides CP203 is a Gram-positive bacterium capable of synthesizing manganese oxide, obtained from soil samples of a former manganese mine site in Changping District, Beijing, through screening, isolation, and purification. This strain can grow in both solid and liquid media containing divalent manganese ions and synthesize manganese oxide. The synthesis of manganese oxide was detected using the LBB method; strains that turned colorless LBB (Leduco Berbelin Blue) solution blue were identified as positive strains and purified for preservation.

[0039] Bacillus mycosis fungoides CP203 appears white on solid culture medium with a rough surface. Under scanning electron microscopy, it appears as short, elliptical rods, approximately 1.3–1.8 μm long and 0.6–0.9 μm wide. Figure 5 A. Among them, Gram staining (+), kinetic test (-), D-ribose hydrolysis test (+), starch hydrolysis test (+), glucose hydrolysis test (+), galactose hydrolysis (+), sucrose hydrolysis test (-), oxidase test (+), catalase test (+), mannitol acid production test (-), root growth (+), protein toxin crystals (-).

[0040] The specific steps for 16S rRNA gene analysis of Bacillus mycosis fungoides CP203 are as follows:

[0041] PCR amplification was performed using universal primers for the 16S rRNA gene, with the following PCR conditions: 98℃ for 2 min; 98℃ for 10 s, 57℃ for 30 min, 72℃ for 40 s, 32 cycles; 72℃ for 10 min. The PCR products were recovered and sequenced (performed by Shanghai Meiji Biotechnology Co., Ltd.). The 16S rRNA gene sequence of CP203 is shown in SEQ ID NO 1. Sequencing results were compared using NCBI BLAST. The phylogenetic tree constructed based on the 16S rRNA gene of CP203 is shown below. Figure 1 Based on 16S rRNA gene identification and physiological and biochemical characteristics, CP203 was identified as *Bacillus mycosis* and named *Bacillus mycosis*. Bacillus mycoides CP203.

[0042] The above-isolated strain of Bacillus mycosis fungoides ( Bacillus mycoides CP203 was deposited on December 28, 2021, at the China General Microbiological Culture Collection Center (CGMCC, Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, 100101, China), with accession number CGMCC No. 24208, and classified as follows: Bacillus mycoides .

[0043] Example 2: Synthesis of manganese oxide by contact oxidation of Bacillus mycosiscin CP203

[0044] Manganese oxide was synthesized by Bacillus CP203 under different MnCl2 concentrations.

[0045] Bacillus cereus CP203 cultured on plates was inoculated into liquid culture medium containing different concentrations of MnCl2. Each 1L of liquid culture medium contained: 2.0 g peptone, 0.5 g yeast extract, 0.15 mol NaCl, 0.005 mol KCl, 0.025 mol MgSO4, 0.005 mol CaCl2, and 1.0-20.0 mmol MnCl2, with a pH of 7.5. 100 ml of culture medium was added to each 250 ml Erlenmeyer flask. A control group was included, which was not inoculated with bacteria. Both experimental and control groups were cultured in the same shaking incubator at 150 rpm and 25℃ for 10 days. After cultivation, the manganese oxide concentration in the culture medium was quantitatively determined using the LBB method. The results are shown below. Figure 2 .

[0046] Depend on Figure 2The results showed that as the MnCl2 concentration increased, the yield of manganese oxide initially increased and then slightly decreased, indicating that high concentrations of Mn(II) inhibited the growth of Bacillus mycoides CP203. The optimal MnCl2 concentration was 5.0-10.0 mmol / L.

[0047] Example 3: Synthesis of manganese oxide by Bacillus mycosis fungoides CP203 via a non-contact method

[0048] Experiment on the oxidation of divalent manganese ions in solid medium by Bacillus mycoides CP203.

[0049] Add Mn(II)-free solid culture medium I to one side of a petri dish and inoculate with Bacillus mycoides CP203. Each 1L of solid culture medium contains: 2.0 g peptone, 0.5 g yeast extract, 0.15 mol NaCl, 0.005 mol KCl, 0.025 mol MgSO4, 0.005 mol CaCl2, and 1.5 g agar. The pH of the culture medium is 7.2. Add MnCl2-containing solid culture medium II to the blank position of the petri dish, with 10.0 mmol of MnCl2 added to each 1L of medium II compared to medium I. Culture media I and II do not come into contact. A control group was included in the experiment. The control group's medium I was not inoculated with any bacteria, and medium II was the same as the experimental group.

[0050] The experimental and control groups were cultured in the same incubator for 10 days at 25°C. From the third day of culture, the experimental group's culture medium II showed a light brown color that gradually deepened. The control group's culture medium II showed no change. After culture, manganese oxide was detected using the LBB method. The experimental group's culture medium II turned blue, while the control group's culture medium II remained colorless, confirming that *Bacillus mycoides* CP203 synthesized manganese oxide via a non-contact method. Experimental results are shown below. Figure 3 The results showed that although Bacillus mycoides CP203 and Mn(II) did not come into contact, manganese oxide was synthesized under the action of Bacillus mycoides CP203.

[0051] Experiment on non-contact oxidation of Mn(II) in liquid medium by Bacillus mycoides CP203.

[0052] Manganese oxide was synthesized non-contactly using a six-well plate. Solid culture medium was added to the two wells on the left and inoculated with *Bacillus mycoides* CP203. Each 1L of solid culture medium contained: 10.0 g peptone; 5.0 g yeast extract; 10.0 g NaCl; and 1.5 g agar. The pH of the culture medium was 7.2. The two wells in the middle were left empty. A solution containing MnCl2 was added to the two wells on the right. Each 1L of the solution contained: 0.15 mol NaCl; 0.005 mol KCl; 0.025 mol MgSO4; 0.005 mol CaCl2; and 1.0–20.0 mmol MnCl2. The pH of the solution was 7.2. A control group was included, in which the solid culture medium was not inoculated with *Bacillus mycoides* CP203, and the MnCl2 concentration was the same as in the experimental group.

[0053] The experimental group and the control group were placed in the same incubator and incubated statically for 10 days at a temperature of 25℃. The results are as follows: Figure 3 As shown in Figure B. From the 4th day of cultivation, the MnCl2 solution showed a brown or black turbidity. After the experiment, the content of manganese oxide produced was determined using the LBB method, and the results are shown in Figure B. Figure 4 As shown, the amount of manganese oxide synthesized increases rapidly with increasing MnCl2 concentration, and then tends to level off. The amount of manganese oxide synthesized is relatively high when MnCl2 concentration is between 5.0 and 20.0 mmol / L.

[0054] Scanning electron micrographs of manganese oxide synthesized by Bacillus mycosis fungoides CP203 via direct and indirect contact methods are shown below. Figure 5 As shown. According to scanning electron microscopy results, the manganese oxide synthesized by the non-contact method has a single component and is not mixed with any bacterial cells. Figure 5 The C). However, the manganese oxide products synthesized through direct contact are tightly bound to the bacterial cells and cannot be separated (C). Figure 5 B).

[0055] Transmission electron microscopy-elemental analysis of manganese oxide synthesized by Bacillus mycosis fungoides CP203 via a non-contact method is shown in [link to image]. Figure 6 According to transmission electron microscopy-elemental analysis, the main components of manganese oxide synthesized by non-contact oxidation are Mn and O, with small amounts of Ca, K, Na, and Mg also included.

[0056] The above results demonstrate that *Bacillus mycosis fungoides* CP203 successfully synthesized manganese oxide via a non-contact method without any contamination by bacterial cells. Compared to direct contact synthesis of manganese oxide, the non-contact synthesis by *Bacillus mycosis fungoides* CP203 is less inhibited by MnCl2 concentration. Furthermore, the non-contact oxidation process only requires isolating the growth of *Bacillus mycosis fungoides* CP203 in the culture medium, eliminating the need to add nutrients directly to the solution containing divalent manganese ions, thus reducing the cost of subsequent microbial sterilization. Additionally, it eliminates the need for bacterial inoculation into the solution, making it particularly suitable for applications requiring the oxidation of divalent manganese ions but where introducing additional bacteria is not advisable.

[0057] Example 4: Effect of Cd(II) on non-contact synthesis of manganese oxide by Bacillus mycosis fungoides CP203

[0058] Cd(II) is a common heavy metal pollutant in the environment and is toxic to microorganisms. Microorganisms can hardly grow in a culture medium containing Cd(II). Therefore, this embodiment did not conduct an experiment on the synthesis of manganese oxide in contact with Cd(II), but only tested the effect of Cd(II) on the synthesis of manganese oxide in non-contact conditions.

[0059] The effect of Cd(II) on the non-contact synthesis of manganese oxide by Bacillus mycosis fungoides CP203 was investigated using a six-well plate. Solid culture medium was added to the two wells on the left and inoculated with Bacillus mycosis fungoides CP203. Each 1L of solid culture medium contained: 10.0 g peptone; 5.0 g yeast extract; 10.0 g NaCl; and 1.5 g agar. The pH of the culture medium was 7.2. The two wells in the middle were left empty. A solution containing MnCl2 and Cd(NO3)2 was added to the two wells on the right. Each 1L of the solution contained: 0.15 mol NaCl; 0.005 mol KCl; 0.025 mol MgSO4; 0.005 mol CaCl2; 10.0 mmol MnCl2; and 0.01–0.1 mmol Cd(NO3)2. The pH of the solution was 7.2. The experiment included a control group, in which Cd(NO3)2 was not added to the MnCl2 solution, and the Bacillus mycoides CP203 inoculated was the same as that in the experimental group.

[0060] The experimental and control groups were placed in the same incubator and incubated statically for 10 days at 25℃. After the experiment, the manganese oxide content was determined using the LBB method, and the results are as follows: Figure 7 As shown.

[0061] Figure 7The results showed that Cd(II) at low concentrations of 0.01–0.05 mmol / L had almost no effect on the non-contact synthesis of manganese oxide by Bacillus mycosis fungi CP203. At higher concentrations of 0.1 mmol / L, the manganese oxide synthesis decreased slightly, by 24.2% compared to the absence of Cd(II). These results indicate that the non-contact synthesis of manganese oxide by Bacillus mycosis fungi CP203 is less affected by other metal ions.

[0062] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A type of fungal spore-forming bacillus ( Bacillus mycoides CP203, characterized in that, The Bacillus mycoides CP203 has the accession number CGMCC No. 24208.

2. A microbial inoculant, characterized in that, It contains Bacillus mycoides CP203 as described in claim 1, or its freeze-dried cells, or its bacterial suspension, or the precipitate resuspension after centrifugation of the bacterial suspension; The microbial agent, Bacillus mycoides CP203, has a viable bacterial content ≥1×10⁻⁶. 8 cfu / mL.

3. A product characterized in that, The product comprises Bacillus mycoides CP203 as described in claim 1, or its freeze-dried cells, or its bacterial suspension, or the precipitate resuspension after centrifugation of the bacterial suspension, or the microbial agent as described in claim 2; The product contains ≥1×10⁻⁶ live Bacillus CP203 bacteria. 8 cfu / mL; The product is a biocatalyst or a wastewater treatment agent.

4. The use of Bacillus mycoides CP203 of claim 1 or its freeze-dried cells or its bacterial solution, or the precipitate resuspension after centrifugation of the bacterial solution, or the microbial agent of claim 2, or the product of claim 3, in the preparation or synthesis of manganese oxide.

5. The application of Bacillus mycoides CP203 of claim 1 or its freeze-dried cells, or its bacterial solution, or the precipitate resuspension after centrifugation of the bacterial solution, or the microbial agent of claim 2, or the product of claim 3, in the treatment of wastewater containing divalent manganese ions.

6. A method for synthesizing manganese oxide, characterized in that, Manganese oxide is synthesized from a solution containing divalent manganese ions using Bacillus mycoides CP203 as described in claim 1, its freeze-dried cells, its bacterial solution, or the precipitate resuspension after centrifugation of the bacterial solution, or the microbial agent as described in claim 2, or the product as described in claim 3, in a direct contact or non-contact manner.

7. The method according to claim 6, characterized in that, The method for directly synthesizing manganese oxide is as follows: adding the Bacillus CP203 of claim 1 or its freeze-dried cells or its bacterial solution, or the precipitate resuspension after centrifugation of the bacterial solution, or the microbial agent of claim 2, or the product of claim 3, into a solution containing divalent manganese ions.

8. The method according to claim 6, characterized in that, The non-contact synthesis method for manganese oxide is as follows: The *Bacillus mycoides* CP203 of claim 1, or its freeze-dried cells, or its bacterial solution, or the precipitate resuspension after centrifugation of the bacterial solution, or the microbial agent of claim 2, or the product of claim 3, are placed in a solution containing divalent manganese ions without contact. This non-contact placement means that the *Bacillus mycoides* CP203 of claim 1, or its freeze-dried cells, or its bacterial solution, or the precipitate resuspension after centrifugation of the bacterial solution, or the microbial agent of claim 2, or the product of claim 3, and the solution containing divalent manganese ions are each open and not sealed, and are in the same space. The temperature of the space is 25-30℃, and the non-contact placement time is 3-10 days.

9. The method according to claim 8, characterized in that, The concentration of manganese ions in the solution containing divalent manganese ions is 1.0-20.0 mmol / L.

10. The method according to claim 9, characterized in that, The concentration of manganese ions in the solution containing divalent manganese ions is 5.0-20.0 mmol / L.

11. A method for treating divalent manganese ions in wastewater, characterized in that, The Bacillus CP203 of claim 1 or its freeze-dried cells or its bacterial solution, or the precipitate resuspension after centrifugation of the bacterial solution, or the microbial agent of claim 2, or the product of claim 3, shall be placed in a non-contact environment with a solution containing divalent manganese ions; wherein, non-contact environment means that the Bacillus CP203 of claim 1 or its freeze-dried cells or its bacterial solution, or the precipitate resuspension after centrifugation of the bacterial solution, or the microbial agent of claim 2, or the product of claim 3, and the wastewater containing divalent manganese ions shall each be placed in an open, non-sealed environment, and in the same space; The temperature of the space is 25-30℃, and the non-contact placement time is 3-10 days.

Images