Method for detecting core bioactive component group entering blood of Wantongkang and verification

Abstract

The invention discloses a method for detecting a Wantongkang in-blood core bioactive component group and verification, serum, urine and excrement are collected as an experimental group after a preset time, and serum, urine and excrement of animals not given with Wantongkang are used as a control group; and detecting the experimental group and the control group by adopting liquid chromatography-mass spectrometry. The invention systematically analyzes the original components and metabolites of Wantongkang in blood plasma, urine and excrement of rats. 38 primordial components are detected in plasma, 48 urine and 89 excrement. The terpenoids may form a core pharmacodynamic material basis of WTYK for treating pharyngitis. A metabolic conversion path of picria fel-terrae glycoside IA, rosmarinic acid, isofraxidin, caffeic acid and apigenin-7-O-glucuronide is constructed through metabolic conversion analysis, and active metabolites of picria fel-terrae glycoside IA, rosmarinic acid, isofraxidin, caffeic acid and The network pharmacological research based on the in-blood component reveals that the WTYK plays a therapeutic role in treating the pharyngitis mainly by regulating key signal channels such as PI3K-Akt, MAPK, TNF, JAK-STAT and NF-kB, wherein the NF-kB signal channel is closely related to the occurrence and development of the pharyngitis.

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