Pet intestinal health and excrement deodorization composite additive and preparation method thereof

Abstract

The invention discloses a pet intestinal health and excrement deodorization composite additive and a preparation method thereof, and belongs to the technical field of pet nutritional supplements.The preparation method comprises the steps that lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis are embedded and then mixed with pericarpium citri reticulatae, radix aucklandiae, cortex magnoliae officinalis, a jerusalem artichoke extract, zeolite and montmorillonite, and then the composite additive is prepared; through formula optimization, integrated application and scientific proportioning of daily ration functional additives, the health of intestinal tracts of cats can be regulated, the oxidation resistance and immune function of animal bodies can be remarkably improved, and convenience is provided for scientific and healthy cat breeding; the beneficial bacteria are subjected to embedding treatment, so that the beneficial bacteria are not influenced by the environment and are not easy to inactivate, and the effective components are better protected and play a better role; functional components capable of covering the odor of pet excrement are added, so that the generation of odor substances can be reduced from the source, and the pet excrement deodorant is suitable for maintaining the intestinal health of pets and deodorizing excrement.

Description

Technical Field

[0001] The present application belongs to the technical field of pet nutritional supplements, and in particular relates to a pet intestinal health and feces deodorization composite additive and a preparation method thereof. Background Art

[0002] The intestines are crucial for nutrient digestion and absorption in dogs and cats, and their health plays a crucial role in overall pet health. The intestinal microbiome is also a crucial determinant of its composition. The intestinal microbiome primarily refers to the composition of the intestinal flora and the stability of the intestinal environment. Over 10 bacterial phyla have been identified in the cat's intestinal microbiome, with Firmicutes, Bacteroidetes, Fusobacteria, Proteobacteria, and Actinobacteria as the dominant phyla. The intestinal microbiome is closely linked to a cat's overall health. Microorganisms convert carbohydrates into short-chain fatty acids, which provide energy for endothelial cells, induce anti-inflammatory effects in regulatory T cells, regulate intestinal motility, and reduce the adhesion of harmful bacteria to the intestine. Gastrointestinal diseases are common in cats, often accompanied by symptoms such as vomiting, diarrhea, anorexia, and weight loss. Improper diet is a major cause of gastrointestinal disease in cats. In diseased states, intestinal antimicrobial peptide and mucus secretion decreases, leading to increased endothelial permeability and bacterial translocation. Toll-like receptors on macrophages and other cells recognize substances such as lipopolysaccharides, triggering an inflammatory response, leading to oxidative stress, and ultimately, an imbalance in the intestinal microbiome. Intestinal flora imbalance in cats can cause diseases such as inflammatory bowel disease, constipation, metabolic syndrome and irritable bowel syndrome.

[0003] The nutrient composition and additives used in pet food are important factors influencing intestinal health. Furthermore, during digestion, cats' intestines produce odorous substances such as hydrogen sulfide, indole, and skatole. Cats also secrete irritating liquid secretions into their feces to mark their territory, and these secretions may contain information about gender, reproductive status, and individual identification. The odor caused by these odorous substances and signaling molecules is one of the main issues plaguing cat owners. Currently, there is limited research on pet intestinal health and fecal deodorization, and the few that exist primarily focus on the use of single additives, rather than integrated applications. Summary of the Invention

[0004] The purpose of the present application is to provide a pet intestinal health and feces deodorization compound additive and its preparation method, so as to solve the technical problems existing in the prior art of pet food additives such as single function, low integration, easy inactivation of effective ingredients and poor effect.

[0005] To achieve the above objectives, the technical solution adopted in this application is to provide a method for preparing a pet intestinal health and feces deodorizing composite additive, which specifically includes the following steps: (1) Component encapsulation: encapsulating Lactobacillus plantarum, Saccharomyces cerevisiae and Bacillus subtilis to obtain encapsulated Lactobacillus plantarum, encapsulated Saccharomyces cerevisiae and encapsulated Bacillus subtilis; (2) Mixing: Preliminarily mix dried tangerine peel, costus root, magnolia bark, Jerusalem artichoke extract, zeolite and montmorillonite, and then add the embedded lactobacillus plantarum, the embedded saccharomyces cerevisiae and the embedded bacillus subtilis to prepare a composite additive.

[0006] In one embodiment, The specific steps of step (1) embedding are: S1. Lactobacillus plantarum, Saccharomyces cerevisiae, and Bacillus subtilis are mixed in a ratio of 1:2:1 and suspended in a composite protective agent solution (15% whey protein concentrate + 5% trehalose + 5% glycerol); S2. Homogenization: Slightly vortex or homogenize under sterile conditions to evenly disperse the bacteria to obtain a mixed bacterial solution; S3. Pre-freeze: Aliquot the mixed bacterial solution into sterile cryopreservation tubes with a thickness of 0.5-1 cm.

[0007] In one embodiment, The specific steps of pre-freezing are: first freezing at -20 °C for 2 h for preliminary solidification, then transferring to a -80 °C ultra-low temperature freezer and freezing for 4 h to form fine ice crystals; the composite protective agent solution is 15% concentrated whey protein, 5% trehalose, and 5% glycerol.

[0008] In one embodiment, The particle size of tangerine peel, costus root, magnolia bark, Jerusalem artichoke extract, zeolite, and montmorillonite is 150 μm.

[0009] In one embodiment, The rotation speed of the preliminary mixing in step (ii) is 800 rpm.

[0010] In one embodiment, In step (ii), the embedded Lactobacillus plantarum, the embedded Saccharomyces cerevisiae and the embedded Bacillus subtilis were added and mixed at a speed of 300 rpm.

[0011] In one embodiment, The number of viable cells of the embedded Lactobacillus plantarum was 1×10 9 CFU / g; the number of live bacteria of the embedded Saccharomyces cerevisiae is 1×10 10 CFU / g; the number of viable bacteria of the embedded Bacillus subtilis is 1×10 8 CFU / g.

[0012] In one embodiment, The growth performance, apparent digestibility, fecal indole, skatole and short-chain fatty acid contents and blood parameters of the composite additive were determined.

[0013] The present application also provides a pet intestinal health and feces deodorization composite additive, which includes the following components by weight: 50-55 parts of tangerine peel, 50-55 parts of costus root, 50-55 parts of magnolia bark, 350-365 parts of Jerusalem artichoke extract, 50-55 parts of embedded Lactobacillus plantarum, 100-110 parts of embedded brewer's yeast, 50-55 parts of embedded Bacillus subtilis, 200-210 parts of zeolite powder, and 100-110 parts of montmorillonite powder.

[0014] Tangerine peel, costus root, and magnolia bark contain aromatic substances, which can play a certain role in masking odors; they are rich in organic acids, and their active group carboxyl can directly adsorb odor components such as ammonia and organic amines. The olefin bonds, carbonyl groups, aldehydes, ketones, and alkylene oxide groups therein can eliminate odors through polymerization and addition reactions; studies have shown that these three medicines have the function of regulating the activity of digestive enzymes, and can significantly affect the digestion and absorption of nitrogen-containing compounds and sulfur-containing compounds by animal bodies, thereby reducing the generation of odorous substances from the source.

[0015] Jerusalem artichoke is a perennial plant of the Asteraceae family, which contains active ingredients such as inulin. It has the effects of improving animals' resistance to stress, increasing digestibility, increasing the number of beneficial intestinal bacteria (such as Lactobacillus) and reducing the number of Escherichia coli, improving intestinal health, and reducing diarrhea rates.

[0016] Lactobacillus plantarum is one of the most extensively studied lactic acid bacteria. It is a Gram-positive lactobacillus that is facultatively heterofermentative. Its metabolites include natural antibacterial substances such as organic acids, bacteriocins, hydrogen peroxide, and diacetyl. These substances inhibit the growth of intestinal pathogens and protect the intestinal microecological balance. It colonizes the adsorption sites of gastrointestinal epithelial cells, occupying an ecological niche and preventing the adsorption of pathogens, thereby protecting the intestine from pathogen invasion. Lactobacillus plantarum can significantly enhance the antioxidant capacity and immune function of animals. Furthermore, Lactobacillus plantarum can increase the apparent digestibility of nitrogenous compounds such as protein and reduce the production of ammonia and hydrogen sulfide in feces. Studies have shown that adding Lactobacillus plantarum to cat diets can significantly reduce the levels of indole and 3-methylindole in feces, lower triglyceride levels in blood, and enhance immunity.

[0017] Saccharomyces cerevisiae is a simple, single-celled microorganism belonging to the fungi family and is facultatively anaerobic. Yeast is rich in nutrients such as protein, B vitamins, nucleic acids, and carbohydrates. Numerous studies have shown that using Saccharomyces cerevisiae as a probiotic can positively impact the intestinal and microbial functions of animals, consistently reducing the incidence of digestive tract diseases. Saccharomyces cerevisiae can exert its effects through its metabolites (antioxidants and B vitamins). Furthermore, the mannan oligosaccharides and β-glucans contained in its cell walls directly exert probiotic effects on intestinal microbes. Numerous studies have confirmed that the addition of Saccharomyces cerevisiae to diets can significantly improve intestinal health in dogs and cats, while also significantly increasing the apparent digestibility of nutrients.

[0018] Bacillus subtilis is a species of the genus Bacillus. During its growth, it produces active substances such as subtilisin, polymyxin, zymogenin, and gramicidin. These substances have significant inhibitory effects on pathogenic bacteria or opportunistic pathogens of endogenous infections. Bacillus subtilis rapidly consumes oxygen from the environment, reducing oxygen levels in the intestine and supporting the growth of beneficial anaerobic bacteria. It also produces organic acids such as lactic acid, which lowers intestinal pH and indirectly inhibits the growth of other pathogens. It can also enhance immune function. It can also synthesize digestive enzymes such as amylase, lipase, protease, and cellulase, which enhance the apparent digestibility of animals in the digestive tract. Studies have shown that the use of Bacillus subtilis can significantly improve fecal scores and reduce the concentration of nitrogen fermentation products (ammonia and phenol) in dog feces.

[0019] Zeolite powder is a hydrous aluminum silicate mineral, with clinoptilolite used in feed. Zeolite protects the gastrointestinal tract, maintains the intestinal mucosal barrier function, enhances digestive enzyme activity, promotes bone development, and improves immunity and antioxidant capacity. Studies have shown that the use of zeolite can significantly reduce odor in dog feces and significantly improve their immune function.

[0020] Montmorillonite is a naturally occurring hydrous layered aluminosilicate mineral with a lamellar structure and non-uniform charge distribution. It forms a protective colloidal film in the digestive tract, exhibiting excellent adsorption for various viruses, bacteria, and the toxins they produce. The mechanisms of interaction between montmorillonite and bacteria can be categorized into two types: adsorption, where the two come into contact and bacteria adhere to the montmorillonite surface; and adsorption of certain chemical substances or groups on the bacterial surface between the montmorillonite layers, resulting in a tighter adhesion of bacterial cells. Therefore, montmorillonite can be used as a carrier for probiotics. Montmorillonite also has numerous other uses, including immobilizing mycotoxins, adsorbing heavy metals, and repairing and protecting the digestive tract mucosa.

[0021] The composite additive prepared by the method provided in this application, through optimized formula, integrated application, and scientific proportioning of functional additives in the diet, can regulate the intestinal health of cats, significantly improve the antioxidant capacity and immune function of the animal body, and provide convenience for scientific and healthy cat breeding; the beneficial bacteria are encapsulated, and the beneficial bacteria are not affected by the environment and are not easily inactivated, so that the effective ingredients are better protected and can better play their role; the addition of functional ingredients that can mask the odor of pet excrement can reduce the generation of odorous substances from the source, and is suitable for maintaining pet intestinal health and deodorizing feces. DETAILED DESCRIPTION

[0022] In order to make the technical problems, technical solutions and beneficial effects to be solved by this application more clear and understandable, this application is further described in detail. It should be understood that the specific embodiments described herein are only used to explain this application and are not used to limit this application.

[0023] Example 1 A method for preparing a pet intestinal health and feces deodorization composite additive comprises the following steps: (I) Component encapsulation: Lactobacillus plantarum, Saccharomyces cerevisiae and Bacillus subtilis were encapsulated to obtain encapsulated Lactobacillus plantarum, encapsulated Saccharomyces cerevisiae and encapsulated Bacillus subtilis; the number of viable cells of the encapsulated Lactobacillus plantarum was 1×10 9 CFU / g; the number of live bacteria of the embedded Saccharomyces cerevisiae is 1×10 10 CFU / g; the number of viable bacteria of the embedded Bacillus subtilis is 1×10 8 CFU / g; The specific steps of embedding are: S1, Lactobacillus plantarum, Saccharomyces cerevisiae and Bacillus subtilis were mixed in a ratio of 1:2:1 and suspended in 100 ml of a composite protective agent solution; the composite protective agent solution was 15% whey protein concentrate, 5% trehalose and 5% glycerol; S2. Homogenization: Slightly vortex or homogenize under sterile conditions (avoid foaming) to evenly disperse the bacteria to obtain a mixed bacterial solution; S3. Prefreeze: Aliquot the mixed bacterial solution into sterile cryovials with a thickness of 0.5-1 cm. Prefreeze as follows: First, freeze at -20°C for 2 hours for initial solidification, then transfer to a -80°C ultra-low temperature freezer and freeze for 4 hours to form fine ice crystals. S4, freeze drying: cold trap temperature: -50 ° C, vacuum degree: 0.1 mbar, primary drying: -40 to -20 ° C, 24 h sublimation to remove free water, secondary drying: 20 ° C, 12 h to remove bound water, final moisture ≤ 5%; (2) Mixing: Tangerine peel with a particle size of 150 μm, costus root, magnolia bark, Jerusalem artichoke extract, zeolite, and montmorillonite are preliminarily mixed in a ratio of 1:1:7:4:2 at a mixing speed of 800 rpm; then, the embedded Lactobacillus plantarum, the embedded Saccharomyces cerevisiae, and the embedded Bacillus subtilis are added and mixed at a mixing speed of 300 rpm to obtain a powdered composite additive.

[0024] Example 2 A pet intestinal health and feces deodorization composite additive comprises the following components, measured by weight: 52 parts of dried tangerine peel, 53 parts of costus root, 52 parts of magnolia bark, 357 parts of Jerusalem artichoke extract, 52 parts of embedded lactobacillus plantarum, 105 parts of embedded saccharomyces cerevisiae, 52 parts of embedded Bacillus subtilis, 205 parts of zeolite powder, and 105 parts of montmorillonite powder.

[0025] Example 3 A pet intestinal health and feces deodorization composite additive comprises the following components, measured by weight: 50 parts of dried tangerine peel, 50 parts of costus root, 50 parts of magnolia bark, 350 parts of Jerusalem artichoke extract, 50 parts of embedded lactobacillus plantarum, 100 parts of embedded saccharomyces cerevisiae, 50 parts of embedded bacillus subtilis, 200 parts of zeolite powder, and 100 parts of montmorillonite powder.

[0026] Example 4 A pet intestinal health and feces deodorization composite additive comprises the following components, measured by weight: 55 parts of dried tangerine peel, 55 parts of costus root, 55 parts of magnolia bark, 365 parts of Jerusalem artichoke extract, 55 parts of embedded lactobacillus plantarum, 110 parts of embedded saccharomyces cerevisiae, 55 parts of embedded bacillus subtilis, 210 parts of zeolite powder, and 110 parts of montmorillonite powder.

[0027] Experimental example Twenty healthy adult cats of similar weight and age were randomly divided into two groups and housed individually with free access to water. The blank group was fed a basal diet, while the experimental group was fed a diet containing the composite additive prepared in Example 1. The experimental period was 60 days.

[0028] 1. Measurement indicators 1-1. Determination of growth performance In the morning of the 1st, 30th and 60th day of the formal experiment, the cats were weighed on an empty stomach, and the daily feed intake and leftover feed were recorded to calculate the average daily feed intake and average daily water intake. The results are shown in Table 1.

[0029] Table 1 Effects of compound additives on cat growth performance

[0030] 1-2. Determination of apparent digestibility Feces were collected on the 30th and 60th days of the formal experiment, once in the morning and once in the afternoon, and the consumed diet was also collected. The apparent digestibility of dry matter, crude protein, and crude fat was determined using the acid-insoluble ash method. The results are shown in Table 2.

[0031] Table 2 Effects of compound additives on apparent digestibility in cats

[0032] 1-3. Feces collection and index measurement During the trial period, stool characteristics were monitored and scored according to Table 3. The scoring results are shown in Table 4.

[0033] Table 3 Stool characteristics scoring table

[0034] Table 4 Effects of compound additives on cat feces score index

[0035] Fresh feces were collected on the 1st, 20th, 40th and 60th days of the formal period, and the contents of indole, skatole and short-chain fatty acids in the feces were measured by liquid chromatography to determine its deodorizing effect. The results are shown in Table 5.

[0036] Table 5 Effects of compound additives on indole, skatole, and short-chain fatty acids in cat feces

[0037] 1-4. Determination of blood indicators In the early morning of the 1st, 30th and 60th days, venous blood was collected from the experimental cats for the determination of blood biochemical and physiological indices. The results are shown in Table 6.

[0038] Table 6 Effects of compound additives on cat blood parameters

[0039] The results showed that the compound additive used in this experiment can significantly improve the feces score of pets, improve their digestion and absorption of nutrients, reduce the content of indole, skatole and short-chain fatty acids in feces, achieve a deodorizing effect, and is highly safe and will not cause harm to the pet's body.

[0040] The present application provides a preparation method of a composite additive for pet intestinal health and feces deodorization, comprising the following steps: component encapsulation: encapsulating Lactobacillus plantarum, Saccharomyces cerevisiae and Bacillus subtilis to obtain embedded Lactobacillus plantarum, embedded Saccharomyces cerevisiae and embedded Bacillus subtilis; mixing: preliminarily mixing dried tangerine peel, costus root, Magnolia bark, Jerusalem artichoke extract, zeolite and montmorillonite, and then adding the embedded Lactobacillus plantarum, embedded Saccharomyces cerevisiae and embedded Bacillus subtilis to prepare a composite additive; the composite additive prepared by the above method, through optimizing the formula and integrated application, scientifically proportioning the functional additives of the diet, can regulate the intestinal health of cats, significantly improve the antioxidant capacity and immune function of the animal body, and provide convenience for scientific and healthy cat breeding; the beneficial bacteria are encapsulated, so that the beneficial bacteria are not affected by the environment and are not easily inactivated, so that the effective ingredients are better protected and play a better role; adding functional ingredients that can mask the odor of pet excrement can reduce the generation of odorous substances from the source, and is suitable for maintaining pet intestinal health and feces deodorization.

[0041] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. Throughout the description of this application, "plurality" means two or more, unless otherwise specifically defined.

[0042] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present application should be included in the scope of protection of the present application.

Claims

1. A method for preparing a pet intestinal health and feces deodorization composite additive, characterized in that: The specific steps include: (1) Component encapsulation: encapsulating Lactobacillus plantarum, Saccharomyces cerevisiae and Bacillus subtilis to obtain encapsulated Lactobacillus plantarum, encapsulated Saccharomyces cerevisiae and encapsulated Bacillus subtilis; (2) Mixing: Preliminarily mix dried tangerine peel, costus root, magnolia bark, Jerusalem artichoke extract, zeolite and montmorillonite, and then add the embedded Lactobacillus plantarum, embedded Saccharomyces cerevisiae and embedded Bacillus subtilis to prepare a composite additive.

2. The method for preparing a pet intestinal health and feces deodorization composite additive according to claim 1, characterized in that: The specific steps of embedding in step (1) are: S1. Mix Lactobacillus plantarum, Saccharomyces cerevisiae and Bacillus subtilis and suspend them in a composite protective agent solution; S2. Homogenization: Slightly vortex or homogenize under sterile conditions to evenly disperse the bacteria to obtain a mixed bacterial solution; S3. Pre-freezing: The mixed bacterial solution is divided into sterile freezing tubes.

3. The method for preparing a pet intestinal health and feces deodorization composite additive according to claim 2, characterized in that: The specific steps of the pre-freezing are: first freezing at -20 ° C for 2 hours for preliminary solidification, then transferring to a -80 ° C ultra-low temperature freezer and freezing for 4 hours to form fine ice crystals; the composite protective agent solution is 15% concentrated whey protein, 5% trehalose, and 5% glycerol; in S3, the thickness of the packaging is 0.5-1 cm.

4. The method for preparing a pet intestinal health and feces deodorization composite additive according to claim 1, characterized in that: The mixing ratio of the plant lactobacillus, saccharomyces cerevisiae and bacillus subtilis is 1:2:

1.

5. The method for preparing a pet intestinal health and feces deodorization composite additive according to claim 1, characterized in that: The particle size of the tangerine peel, costus root, magnolia bark, Jerusalem artichoke extract, zeolite, and montmorillonite is 150 μm, and the mixing ratio of the tangerine peel, costus root, magnolia bark, Jerusalem artichoke extract, zeolite, and montmorillonite is 1:1:7:4:

2.

6. The method for preparing a pet intestinal health and feces deodorization composite additive according to claim 1, characterized in that: The rotation speed of the preliminary mixing in step (2) is 800 rpm.

7. The method for preparing a pet intestinal health and feces deodorization composite additive according to claim 1, characterized in that: In step (ii), the embedded Lactobacillus plantarum, the embedded Saccharomyces cerevisiae and the embedded Bacillus subtilis were added and mixed at a speed of 300 rpm.

8. The method for preparing a pet intestinal health and feces deodorization composite additive according to claim 1, characterized in that: The number of live bacteria of the embedded Lactobacillus plantarum is 1×10 9 CFU / g; the number of live bacteria of the embedded Saccharomyces cerevisiae is 1×10 10 CFU / g; the number of viable bacteria of the embedded Bacillus subtilis is 1×10 8 CFU / g.

9. The method for preparing a pet intestinal health and feces deodorization composite additive according to claim 1, characterized in that: The growth performance, apparent digestibility, contents of indole, skatole and short-chain fatty acids in feces and blood indices of the composite additive were measured.

10. A pet intestinal health and feces deodorization composite additive, characterized in that: The composite additive includes the following components by weight: 50-55 parts of dried tangerine peel, 50-55 parts of costus root, 50-55 parts of magnolia bark, 350-365 parts of Jerusalem artichoke extract, 50-55 parts of embedded plant lactobacillus, 100-110 parts of embedded brewer's yeast, 50-55 parts of embedded bacillus subtilis, 200-210 parts of zeolite powder, and 100-110 parts of montmorillonite powder.

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