Lung clearing granule and preparation method thereof

Abstract

The invention discloses lung heat clearing granules and a preparation method thereof, and relates to the technical field of pharmaceutical preparations. The lung heat clearing granule is prepared from the following components in parts by weight: 30 to 50 parts of traditional Chinese medicine preparation, 10 to 15 parts of hesperidin, 5 to 10 parts of flos farfarae polysaccharide, 2 to 6 parts of oridonin, 1 to 3 parts of breviscapine, 1 to 3 parts of maltodextrin and 100 to 200 parts of water, the traditional Chinese medicine preparation comprises the following components in parts by weight: 10-15 parts of Chinese dates, 10-15 parts of scutellaria baicalensis, 5-10 parts of platycodon grandiflorum, 5-10 parts of poria cocos, 5-10 parts of exocarpium citri rubrum, 5-10 parts of thunberg fritillary bulb, 5-10 parts of white mulberry root-bark, 3-8 parts of angelica sinensis, 3-8 parts of asparagus fern, 3-8 parts of gardenia, 3-8 parts of scalded bitter apricot kernels, 3-8 parts of radix ophiopogonis, 3-8 parts of ginger, 2-6 parts of liquorice and 1-4 parts of schisandra chinensis. The prepared lung-heat-clearing granules are free of toxic and side effects, good in medicine effect, simple in preparation process and suitable for large-scale popularization.

Description

Technical Field

[0001] The present invention relates to the technical field of pharmaceutical preparations, and in particular to lung-clearing granules and a preparation method thereof. Background Art

[0002] The lungs are the organ most in contact with the external environment and are most susceptible to accumulating toxins. People breathe approximately 10,000 liters of air daily, and various harmful substances in the air enter the lungs through the throat, leading to various respiratory and lung diseases. With urbanization and increasing population density, and increasing personal work pressure, many people suffer from sub-health and weakened immune systems. Air pollution caused by industrial development has made respiratory diseases increasingly common and severe, especially with epidemic pneumonia, which primarily targets the lung's immune system. Smoking, mining, and allergies to industrial waste gas and dust can also cause lung damage, necessitating lung care. Qingfei refers to a method for treating lung heat and yin deficiency. It is commonly used for symptoms such as sore throat and diphtheria caused by yin deficiency, cough due to exertion, dry cough with little sputum, occasional blood streaks, low-grade fever in the afternoon, chest tightness and dull pain, and dry mouth and red tongue. Although there are many traditional Chinese medicines and Western medicines for lung clearing on the market, they generally have problems such as ineffective lung clearing, delayed effects, recurrent attacks after discontinuation of the medicine, and difficulty in long-term use. Therefore, it is urgent to develop a lung clearing granule with a simple preparation process, significant therapeutic effect, and no toxic side effects, and its preparation method. Summary of the Invention

[0003] In view of the above-mentioned defects of the prior art, the present invention proposes Qingfei granules and a preparation method thereof to solve the problems raised by the above-mentioned background technology.

[0004] In order to achieve the above object, the present invention provides the following technical solutions:

[0005] A lung-clearing granule comprising the following ingredients by weight: 30-50 parts of a traditional Chinese medicine, 10-15 parts of hesperidin, 5-10 parts of coltsfoot flower polysaccharide (the flower buds of Tussilago farfara L., a plant of the Asteraceae family), 2-6 parts of rubescens acetoin (the dried aerial parts of Rabdosia rubescens (Hemsl.) Hara, a plant of the Lamiaceae family), 1-3 parts of breviscapine, 1-3 parts of maltodextrin, and 100-200 parts of water;

[0006] The Chinese medicine comprises the following ingredients by weight: 10 to 15 parts of jujube, 10 to 15 parts of scutellaria, 5 to 10 parts of platycodon, 5 to 10 parts of poria, 5 to 10 parts of tangerine peel, 5 to 10 parts of thunbergia bulb, 5 to 10 parts of mulberry bark, 3 to 8 parts of angelica, 3 to 8 parts of asparagus, 3 to 8 parts of gardenia, 3 to 8 parts of stewed bitter almonds, 3 to 8 parts of ophiopogon, 3 to 8 parts of ginger, 2 to 6 parts of liquorice and 1 to 4 parts of schisandra.

[0007] Preferably, the Qingfei granules comprise the following ingredients by weight: 45 parts of traditional Chinese medicine, 12 parts of hesperidin, 9 parts of coltsfoot polysaccharide, 5 parts of oridonin, 2 parts of breviscapine, 2 parts of maltodextrin and 180 parts of water;

[0008] The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra.

[0009] Preferably, the bitter almonds are stewed by putting the bitter almonds into boiling water and boiling for 5 to 10 minutes, quickly taking them out and rubbing off the seed coat, and then drying them for later use.

[0010] Preferably, the above-mentioned ingredients are all purchased from regular channels, can be taken by humans, and are non-toxic.

[0011] Preferably, the preparation method of the traditional Chinese medicine comprises the following steps:

[0012] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0013] (2) adding water to the Chinese medicine mixture, soaking for 25 to 45 minutes, extracting at a temperature of 95 to 100° C. for 50 to 80 minutes, and filtering to obtain a first filtrate and medicinal residues;

[0014] (3) adding water to the medicinal residue obtained in step (2), extracting at a temperature of 95-100° C. for 30-50 min, filtering to obtain a second filtrate for later use;

[0015] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.15 to 1.23, which is a traditional Chinese medicine.

[0016] Preferably, the mass volume ratio of the traditional Chinese medicine mixture and water in step (2) is 1g: (8-15)mL.

[0017] Preferably, the mass volume ratio of the medicinal residue and water in step (3) is 1g:(5-8)mL.

[0018] Preferably, the temperature during the reduced pressure concentration in step (4) is 60-80°C

[0019] Preferably, the method for preparing the coltsfoot flower polysaccharide comprises the following steps:

[0020] 1) drying and crushing coltsfoot flowers, adding a complex enzyme of 2-5% by weight of coltsfoot pollen and water 50-60 times by weight of the coltsfoot pollen, stirring evenly, and performing enzymolysis for 40-50 minutes, then inactivating the enzyme to obtain an enzymolyzate;

[0021] 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 20 to 40 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution;

[0022] 3) adding 4 to 6 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), performing alcohol precipitation at 2 to 4° C. for 20 to 30 hours, and then centrifuging, removing the supernatant and obtaining the precipitate, and freeze-drying the precipitate to obtain coltsfoot flower polysaccharide.

[0023] Preferably, the complex enzyme in step 1) consists of neutral protease and cellulase in an equal mass ratio.

[0024] Preferably, the power of the ultrasonic extraction in step 2) is 150-200W and the temperature is 30-50°C.

[0025] Preferably, the preparation method of Qingfei granules comprises the following steps:

[0026] S1. Mix the Chinese medicinal agent, hesperidin, coltsfoot flower polysaccharide and water, heat to 60-70° C. while stirring, and then cool to obtain a premix for later use;

[0027] S2. Rubescensine A and breviscapine are first added to the premix obtained in step S1 and stirred and mixed, and then maltodextrin is added and stirred to obtain Qingfei granules.

[0028] Preferably, the Qingfei granules are prepared into wet granules by belt drying at 60°C for 1.5 hours, and the Qingfei granules are prepared into dry granules by screening with an 18-mesh sieve, packaging, sampling and full inspection, and storage.

[0029] The present application relates to the use of Qingfei Granules in the preparation of products for promoting lung metabolism, reducing inflammatory factors or relieving asthma.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] (1) The present application selects hesperidin, coltsfoot polysaccharide oridonin and breviscapine as ingredients, and by regulating the dosage of each ingredient and combining them rationally and scientifically, they can synergistically further enhance the lung-clearing effect of the traditional Chinese medicine itself, while having the effects of reducing the content of inflammatory factors and relieving asthma.

[0032] (2) The Qingfei granules prepared in this application have no toxic side effects, good efficacy, simple preparation process, and are suitable for large-scale promotion. DETAILED DESCRIPTION

[0033] The technical solution of the present invention will be further described in detail below in conjunction with specific implementation methods.

[0034] Example 1

[0035] A lung-clearing granule, comprising the following ingredients by weight: 30 parts of a traditional Chinese medicine, 10 parts of hesperidin, 5 parts of coltsfoot flower polysaccharide, 2 parts of oridonin, 1 part of breviscapine, 1 part of maltodextrin, and 100 parts of water;

[0036] The Chinese medicine comprises the following ingredients by weight: 10 parts of jujube, 10 parts of scutellaria, 5 parts of platycodon, 5 parts of poria, 5 parts of tangerine peel, 5 parts of thunbergia bulb, 5 parts of mulberry bark, 3 parts of angelica, 3 parts of asparagus, 3 parts of gardenia, 3 parts of stewed bitter almond, 3 parts of ophiopogon, 3 parts of ginger, 2 parts of liquorice and 1 part of schisandra chinensis.

[0037] The preparation method of the Chinese medicine comprises the following steps:

[0038] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0039] (2) adding water to the Chinese herbal medicine mixture, soaking for 25 minutes, extracting at 95° C. for 50 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal medicine mixture to water is 1 g:8 mL;

[0040] (3) adding water to the medicinal residue obtained in step (2), extracting at 95° C. for 30 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:5 mL;

[0041] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.15, which is a traditional Chinese medicine.

[0042] The preparation method of the coltsfoot flower polysaccharide comprises the following steps:

[0043] 1) drying and crushing coltsfoot flowers, adding a composite enzyme (2% by weight of coltsfoot pollen) and water (50 times the weight of the coltsfoot pollen), stirring evenly, performing enzymolysis for 40 minutes, and then killing the enzyme to obtain an enzymolyzate; the composite enzyme comprises a neutral protease and a cellulase in an equal weight ratio;

[0044] 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 20 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution; the ultrasonic extraction power is 150W and the temperature is 30°C;

[0045] 3) adding 4 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), subjecting the mixture to alcohol precipitation at 2° C. for 20 h, and then centrifuging the mixture, removing the supernatant and obtaining the precipitate, and freeze-drying the precipitate to obtain coltsfoot flower polysaccharide.

[0046] The preparation method of the Qingfei granules comprises the following steps:

[0047] S1. Mix the Chinese medicinal agent, hesperidin, coltsfoot flower polysaccharide and water, heat to 60° C. while stirring, and then cool to obtain a premix for later use;

[0048] S2. Rubescensine A and breviscapine are first added to the premix obtained in step S1 and stirred and mixed, and then maltodextrin is added and stirred to obtain Qingfei granules.

[0049] Example 2

[0050] A lung-clearing granule, comprising the following ingredients by weight: 50 parts of a traditional Chinese medicine, 15 parts of hesperidin, 10 parts of coltsfoot flower polysaccharide, 6 parts of oridonin, 3 parts of breviscapine, 3 parts of maltodextrin, and 200 parts of water;

[0051] The Chinese medicine comprises the following ingredients by weight: 15 parts of jujube, 15 parts of scutellaria, 10 parts of platycodon, 10 parts of poria, 10 parts of tangerine peel, 10 parts of thunbergia bulb, 10 parts of mulberry bark, 8 parts of angelica, 8 parts of asparagus, 8 parts of gardenia, 8 parts of stewed bitter almond, 8 parts of ophiopogon, 8 parts of ginger, 6 parts of liquorice and 4 parts of schisandra.

[0052] The preparation method of the Chinese medicine comprises the following steps:

[0053] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0054] (2) adding water to the Chinese herbal mixture, soaking for 45 minutes, extracting at 100° C. for 80 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal mixture to water is 1 g:15 mL;

[0055] (3) adding water to the medicinal residue obtained in step (2), extracting at a temperature of 100° C. for 50 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:8 mL;

[0056] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.23, which is a traditional Chinese medicine.

[0057] The preparation method of the coltsfoot flower polysaccharide comprises the following steps:

[0058] 1) drying and crushing coltsfoot flowers, adding a composite enzyme (5% by weight of the coltsfoot pollen) and water (60 times the weight of the coltsfoot pollen), stirring evenly, performing enzymatic hydrolysis for 50 minutes, and then inactivating the enzyme to obtain an enzymolyzate; the composite enzyme comprises a neutral protease and a cellulase in an equal weight ratio;

[0059] 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 40 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution; the ultrasonic extraction power is 200 W and the temperature is 50° C.;

[0060] 3) adding 6 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), subjecting the mixture to alcohol precipitation at 4° C. for 30 h, and then centrifuging the mixture, removing the supernatant and obtaining the precipitate, and freeze-drying the precipitate to obtain coltsfoot flower polysaccharide.

[0061] The preparation method of Qingfei granules comprises the following steps:

[0062] S1. Mix the Chinese medicinal agent, hesperidin, coltsfoot flower polysaccharide and water, heat to 70° C. while stirring, and then cool to obtain a premix for later use;

[0063] S2. Rubescensine A and breviscapine are first added to the premix obtained in step S1 and stirred and mixed, and then maltodextrin is added and stirred to obtain Qingfei granules.

[0064] Example 3

[0065] A lung-clearing granule, comprising the following ingredients by weight: 45 parts of a traditional Chinese medicine, 12 parts of hesperidin, 9 parts of coltsfoot flower polysaccharide, 5 parts of oridonin, 2 parts of breviscapine, 2 parts of maltodextrin, and 180 parts of water;

[0066] The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra chinensis.

[0067] The preparation method of the Chinese medicine comprises the following steps:

[0068] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0069] (2) adding water to the Chinese herbal mixture, soaking for 30 minutes, extracting at 98° C. for 60 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal mixture to water is 1 g:10 mL;

[0070] (3) adding water to the medicinal residue obtained in step (2), extracting at 98° C. for 40 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:6 mL;

[0071] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.20, which is a traditional Chinese medicine.

[0072] The preparation method of the coltsfoot flower polysaccharide comprises the following steps:

[0073] 1) drying and crushing coltsfoot flowers, adding a composite enzyme (4% by weight of coltsfoot pollen) and water (55 times the weight of the coltsfoot pollen), stirring evenly, performing enzymolysis for 45 minutes, and then inactivating the enzyme to obtain an enzymolyzate; the composite enzyme comprises a neutral protease and a cellulase in an equal weight ratio;

[0074] 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 30 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution; the ultrasonic extraction power is 180W and the temperature is 40°C;

[0075] 3) adding 5 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), performing alcohol precipitation at 3° C. for 25 h, and then centrifuging, removing the supernatant and obtaining the precipitate, and freeze-drying the precipitate to obtain coltsfoot flower polysaccharide.

[0076] The preparation method of Qingfei granules comprises the following steps:

[0077] S1. Mix the Chinese medicinal agent, hesperidin, coltsfoot flower polysaccharide and water, heat to 65° C. while stirring, and then cool to obtain a premix for later use;

[0078] S2. Rubescensine A and breviscapine are first added to the premix obtained in step S1 and stirred and mixed, and then maltodextrin is added and stirred to obtain Qingfei granules.

[0079] Comparative Example 1

[0080] This comparative example differs from Example 3 in that the amount of the Chinese medicinal preparation is increased, while the other ingredients and steps remain unchanged.

[0081] A lung-clearing granule, comprising the following ingredients by weight: 100 parts of a traditional Chinese medicine, 12 parts of hesperidin, 9 parts of coltsfoot flower polysaccharide, 5 parts of oridonin, 2 parts of breviscapine, 2 parts of maltodextrin, and 180 parts of water;

[0082] The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra chinensis.

[0083] The preparation method of the Chinese medicine comprises the following steps:

[0084] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0085] (2) adding water to the Chinese herbal mixture, soaking for 30 minutes, extracting at 98° C. for 60 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal mixture to water is 1 g:10 mL;

[0086] (3) adding water to the medicinal residue obtained in step (2), extracting at 98° C. for 40 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:6 mL;

[0087] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.20, which is a traditional Chinese medicine.

[0088] The preparation method of the coltsfoot flower polysaccharide comprises the following steps:

[0089] 1) drying and crushing coltsfoot flowers, adding a composite enzyme (4% by weight of coltsfoot pollen) and water (55 times the weight of the coltsfoot pollen), stirring evenly, performing enzymolysis for 45 minutes, and then inactivating the enzyme to obtain an enzymolyzate; the composite enzyme comprises a neutral protease and a cellulase in an equal weight ratio;

[0090] 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 30 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution; the ultrasonic extraction power is 180W and the temperature is 40°C;

[0091] 3) adding 5 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), performing alcohol precipitation at 3° C. for 25 h, and then centrifuging, removing the supernatant and obtaining the precipitate, and freeze-drying the precipitate to obtain coltsfoot flower polysaccharide.

[0092] The preparation method of Qingfei granules comprises the following steps:

[0093] S1. Mix the Chinese medicinal agent, hesperidin, coltsfoot flower polysaccharide and water, heat to 65° C. while stirring, and then cool to obtain a premix for later use;

[0094] S2. Rubescensine A and breviscapine are first added to the premix obtained in step S1 and stirred and mixed, and then maltodextrin is added and stirred to obtain Qingfei granules.

[0095] Comparative Example 2

[0096] The difference between this comparative example and Example 3 is that the Qingfei granules are only a traditional Chinese medicine, and the other ingredients and steps remain unchanged.

[0097] A lung-clearing granule, comprising the following ingredients by weight: 45 parts of a traditional Chinese medicine, 2 parts of maltodextrin, and 180 parts of water;

[0098] The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra.

[0099] The preparation method of the Chinese medicine comprises the following steps:

[0100] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0101] (2) adding water to the Chinese herbal mixture, soaking for 30 minutes, extracting at 98° C. for 60 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal mixture to water is 1 g:10 mL;

[0102] (3) adding water to the medicinal residue obtained in step (2), extracting at 98° C. for 40 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:6 mL;

[0103] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.20, which is a traditional Chinese medicine.

[0104] The preparation method of Qingfei granules comprises the following steps:

[0105] S1. Mix the Chinese medicine and water, heat to 65°C while stirring, and then cool to obtain a premix for later use;

[0106] S2. Add maltodextrin to the premix obtained in step S1, stir, and granulate to obtain Qingfei granules.

[0107] Comparative Example 3

[0108] This comparative example differs from Example 3 in that hesperidin is replaced with Momordica grosvenori extract, while the other ingredients and steps remain unchanged.

[0109] A lung-clearing granule, comprising the following ingredients by weight: 45 parts of a traditional Chinese medicine, 12 parts of Momordica grosvenori extract, 9 parts of coltsfoot flower polysaccharide, 5 parts of oridonin, 2 parts of breviscapine, 2 parts of maltodextrin, and 180 parts of water;

[0110] The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra.

[0111] The preparation method of the Chinese medicine comprises the following steps:

[0112] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0113] (2) adding water to the Chinese herbal mixture, soaking for 30 minutes, extracting at 98° C. for 60 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal mixture to water is 1 g:10 mL;

[0114] (3) adding water to the medicinal residue obtained in step (2), extracting at 98° C. for 40 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:6 mL;

[0115] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.20, which is a traditional Chinese medicine.

[0116] The preparation method of the coltsfoot flower polysaccharide comprises the following steps:

[0117] 1) drying and crushing coltsfoot flowers, adding a composite enzyme (4% by weight of coltsfoot pollen) and water (55 times the weight of the coltsfoot pollen), stirring evenly, performing enzymolysis for 45 minutes, and then inactivating the enzyme to obtain an enzymolyzate; the composite enzyme comprises a neutral protease and a cellulase in an equal weight ratio;

[0118] 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 30 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution; the ultrasonic extraction power is 180W and the temperature is 40°C;

[0119] 3) adding 5 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), performing alcohol precipitation at 3° C. for 25 h, and then centrifuging, removing the supernatant and obtaining the precipitate, and freeze-drying the precipitate to obtain coltsfoot flower polysaccharide.

[0120] The preparation method of Qingfei granules comprises the following steps:

[0121] S1. Mix the Chinese medicinal preparation, Momordica grosvenori extract, coltsfoot flower polysaccharide and water, heat to 65° C. while stirring, and then cool to obtain a premix for later use;

[0122] S2. Rubescensine A and breviscapine are first added to the premix obtained in step S1 and stirred and mixed, and then maltodextrin is added and stirred to obtain Qingfei granules.

[0123] Comparative Example 4

[0124] This comparative example differs from Example 3 in that the composition does not contain hesperidin, and the other ingredients and steps remain unchanged.

[0125] A lung-clearing granule, comprising the following ingredients by weight: 45 parts of a traditional Chinese medicine, 9 parts of coltsfoot flower polysaccharide, 5 parts of oridonin, 2 parts of breviscapine, 2 parts of maltodextrin, and 180 parts of water;

[0126] The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra chinensis.

[0127] The preparation method of the Chinese medicine comprises the following steps:

[0128] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0129] (2) adding water to the Chinese herbal mixture, soaking for 30 minutes, extracting at 98° C. for 60 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal mixture to water is 1 g:10 mL;

[0130] (3) adding water to the medicinal residue obtained in step (2), extracting at 98° C. for 40 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:6 mL;

[0131] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.20, which is a traditional Chinese medicine.

[0132] The preparation method of the coltsfoot flower polysaccharide comprises the following steps:

[0133] 1) drying and crushing coltsfoot flowers, adding a composite enzyme (4% by weight of coltsfoot pollen) and water (55 times the weight of the coltsfoot pollen), stirring evenly, performing enzymolysis for 45 minutes, and then inactivating the enzyme to obtain an enzymolyzate; the composite enzyme comprises a neutral protease and a cellulase in an equal weight ratio;

[0134] 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 30 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution; the ultrasonic extraction power is 180W and the temperature is 40°C;

[0135] 3) adding 5 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), performing alcohol precipitation at 3° C. for 25 h, and then centrifuging, removing the supernatant and obtaining the precipitate, and freeze-drying the precipitate to obtain coltsfoot flower polysaccharide.

[0136] The preparation method of Qingfei granules comprises the following steps:

[0137] S1. Mix the Chinese medicinal agent, coltsfoot flower polysaccharide and water, heat to 65° C. while stirring, and then cool to obtain a premix for later use;

[0138] S2. Rubescensine A and breviscapine are first added to the premix obtained in step S1 and stirred and mixed, and then maltodextrin is added and stirred to obtain Qingfei granules.

[0139] Comparative Example 5

[0140] This comparative example differs from Example 3 in that coltsfoot flower polysaccharide is replaced with honeysuckle flower polysaccharide, and the other ingredients and steps remain unchanged.

[0141] A lung-clearing granule, comprising the following ingredients by weight: 45 parts of a traditional Chinese medicine, 12 parts of hesperidin, 9 parts of honeysuckle polysaccharide, 5 parts of oridonin, 2 parts of breviscapine, 2 parts of maltodextrin, and 180 parts of water;

[0142] The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra chinensis.

[0143] The preparation method of the Chinese medicine comprises the following steps:

[0144] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0145] (2) adding water to the Chinese herbal mixture, soaking for 30 minutes, extracting at 98° C. for 60 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal mixture to water is 1 g:10 mL;

[0146] (3) adding water to the medicinal residue obtained in step (2), extracting at 98° C. for 40 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:6 mL;

[0147] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.20, which is a traditional Chinese medicine.

[0148] The preparation method of the honeysuckle polysaccharide comprises the following steps:

[0149] 1) drying and crushing honeysuckle, adding a composite enzyme (4% by weight of honeysuckle powder) and 55 times the weight of water of the honeysuckle powder, stirring evenly, performing enzymolysis for 45 minutes, and then inactivating the enzyme to obtain an enzymolyzate; the composite enzyme comprises a neutral protease and a cellulase in an equal weight ratio;

[0150] 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 30 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution; the ultrasonic extraction power is 180W and the temperature is 40°C;

[0151] 3) adding 5 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), precipitating the solution with alcohol at 3° C. for 25 h, and then centrifuging the solution. The supernatant was removed to obtain the precipitate, and the precipitate was freeze-dried to obtain honeysuckle polysaccharide.

[0152] The preparation method of Qingfei granules comprises the following steps:

[0153] S1. Mix the Chinese medicinal agent, hesperidin, honeysuckle polysaccharide and water, heat to 65° C. while stirring, and then cool to obtain a premix for later use;

[0154] S2. Rubescensine A and breviscapine are first added to the premix obtained in step S1 and stirred and mixed, and then maltodextrin is added and stirred to obtain Qingfei granules.

[0155] Comparative Example 6

[0156] This comparative example differs from Example 3 in that: the ingredients do not contain coltsfoot flower polysaccharide, and the other ingredients and steps remain unchanged.

[0157] A lung-clearing granule, comprising the following ingredients by weight: 45 parts of a traditional Chinese medicine, 12 parts of hesperidin, 5 parts of oridonin, 2 parts of breviscapine, 2 parts of maltodextrin, and 180 parts of water;

[0158] The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra chinensis.

[0159] The preparation method of the Chinese medicine comprises the following steps:

[0160] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0161] (2) adding water to the Chinese herbal mixture, soaking for 30 minutes, extracting at 98° C. for 60 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal mixture to water is 1 g:10 mL;

[0162] (3) adding water to the medicinal residue obtained in step (2), extracting at 98° C. for 40 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:6 mL;

[0163] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.20, which is a traditional Chinese medicine.

[0164] The preparation method of Qingfei granules comprises the following steps:

[0165] S1. Mix the Chinese medicinal preparation, hesperidin and water, heat to 65° C. while stirring, and then cool to obtain a premix for later use;

[0166] S2. Rubescensine A and breviscapine are first added to the premix obtained in step S1 and stirred and mixed, and then maltodextrin is added and stirred to obtain Qingfei granules.

[0167] Comparative Example 7

[0168] This comparative example differs from Example 3 in that oridonin is replaced with loquat leaf extract, and the other ingredients and steps remain unchanged. A lung-clearing granule, comprising the following ingredients by weight: 45 parts of a traditional Chinese medicine, 12 parts of hesperidin, 9 parts of coltsfoot flower polysaccharide, 5 parts of loquat leaf extract, 2 parts of breviscapine, 2 parts of maltodextrin, and 180 parts of water;

[0169] The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra chinensis.

[0170] The preparation method of the Chinese medicine comprises the following steps:

[0171] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0172] (2) adding water to the Chinese herbal mixture, soaking for 30 minutes, extracting at 98° C. for 60 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal mixture to water is 1 g:10 mL;

[0173] (3) adding water to the medicinal residue obtained in step (2), extracting at 98° C. for 40 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:6 mL;

[0174] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.20, which is a traditional Chinese medicine.

[0175] The preparation method of the coltsfoot flower polysaccharide comprises the following steps:

[0176] 1) drying and crushing coltsfoot flowers, adding a composite enzyme (4% by weight of coltsfoot pollen) and water (55 times the weight of the coltsfoot pollen), stirring evenly, performing enzymolysis for 45 minutes, and then inactivating the enzyme to obtain an enzymolyzate; the composite enzyme comprises a neutral protease and a cellulase in an equal weight ratio;

[0177] 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 30 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution; the ultrasonic extraction power is 180W and the temperature is 40°C;

[0178] 3) adding 5 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), performing alcohol precipitation at 3° C. for 25 h, and then centrifuging, removing the supernatant and obtaining the precipitate, and freeze-drying the precipitate to obtain coltsfoot flower polysaccharide.

[0179] The preparation method of Qingfei granules comprises the following steps:

[0180] S1. Mix the Chinese medicinal agent, hesperidin, coltsfoot flower polysaccharide and water, heat to 65° C. while stirring, and then cool to obtain a premix for later use;

[0181] S2. Add loquat leaf extract and breviscapine to the premix obtained in step S1, stir and mix, then add maltodextrin, stir, and granulate to obtain Qingfei granules.

[0182] Comparative Example 8

[0183] This comparative example differs from Example 3 in that the ingredient is Rubescensine A, and the other ingredients and steps remain unchanged.

[0184] A lung-clearing granule, comprising the following ingredients by weight: 45 parts of a traditional Chinese medicine, 12 parts of hesperidin, 9 parts of coltsfoot flower polysaccharide, 2 parts of breviscapine, 2 parts of maltodextrin, and 180 parts of water;

[0185] The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra.

[0186] The preparation method of the Chinese medicine comprises the following steps:

[0187] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0188] (2) adding water to the Chinese herbal mixture, soaking for 30 minutes, extracting at 98° C. for 60 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal mixture to water is 1 g:10 mL;

[0189] (3) adding water to the medicinal residue obtained in step (2), extracting at 98° C. for 40 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:6 mL;

[0190] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.20, which is a traditional Chinese medicine.

[0191] The preparation method of the coltsfoot flower polysaccharide comprises the following steps:

[0192] 1) drying and crushing coltsfoot flowers, adding a composite enzyme (4% by weight of coltsfoot pollen) and water (55 times the weight of the coltsfoot pollen), stirring evenly, performing enzymolysis for 45 minutes, and then inactivating the enzyme to obtain an enzymolyzate; the composite enzyme comprises a neutral protease and a cellulase in an equal weight ratio;

[0193] 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 30 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution; the ultrasonic extraction power is 180W and the temperature is 40°C;

[0194] 3) adding 5 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), performing alcohol precipitation at 3° C. for 25 h, and then centrifuging, removing the supernatant and obtaining the precipitate, and freeze-drying the precipitate to obtain coltsfoot flower polysaccharide.

[0195] The preparation method of Qingfei granules comprises the following steps:

[0196] S1. Mix the Chinese medicinal agent, hesperidin, coltsfoot flower polysaccharide and water, heat to 65° C. while stirring, and then cool to obtain a premix for later use;

[0197] S2. First, add breviscapine to the premix obtained in step S1 and stir to mix, then add maltodextrin and stir to granulate to obtain Qingfei granules.

[0198] Comparative Example 9

[0199] This comparative example differs from Example 3 in that breviscapine is replaced with lily extract, and the other ingredients and steps remain unchanged.

[0200] A lung-clearing granule, comprising the following ingredients by weight: 45 parts of a traditional Chinese medicine, 12 parts of hesperidin, 9 parts of coltsfoot flower polysaccharide, 5 parts of oridonin, 2 parts of lily extract, 2 parts of maltodextrin, and 180 parts of water;

[0201] The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra.

[0202] The preparation method of the Chinese medicine comprises the following steps:

[0203] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0204] (2) adding water to the Chinese herbal mixture, soaking for 30 minutes, extracting at 98° C. for 60 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal mixture to water is 1 g:10 mL;

[0205] (3) adding water to the medicinal residue obtained in step (2), extracting at 98° C. for 40 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:6 mL;

[0206] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.20, which is a traditional Chinese medicine.

[0207] The preparation method of the coltsfoot flower polysaccharide comprises the following steps:

[0208] 1) drying and crushing coltsfoot flowers, adding a composite enzyme (4% by weight of coltsfoot pollen) and water (55 times the weight of the coltsfoot pollen), stirring evenly, performing enzymolysis for 45 minutes, and then inactivating the enzyme to obtain an enzymolyzate; the composite enzyme comprises a neutral protease and a cellulase in an equal weight ratio;

[0209] 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 30 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution; the ultrasonic extraction power is 180W and the temperature is 40°C;

[0210] 3) adding 5 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), performing alcohol precipitation at 3° C. for 25 h, and then centrifuging, removing the supernatant and obtaining the precipitate, and freeze-drying the precipitate to obtain coltsfoot flower polysaccharide.

[0211] The preparation method of Qingfei granules comprises the following steps:

[0212] S1. Mix the Chinese medicinal agent, hesperidin, coltsfoot flower polysaccharide and water, heat to 65° C. while stirring, and then cool to obtain a premix for later use;

[0213] S2. First, add Rubescensine A and lily extract to the premix obtained in step S1, stir and mix, then add maltodextrin, stir, and granulate to obtain Qingfei granules.

[0214] Comparative Example 10

[0215] This comparative example differs from Example 3 in that there is no breviscapine in the ingredients, and the other ingredients and steps remain unchanged.

[0216] A lung-clearing granule, comprising the following ingredients by weight: 45 parts of a traditional Chinese medicine, 12 parts of hesperidin, 9 parts of coltsfoot flower polysaccharide, 5 parts of oridonin, 2 parts of maltodextrin, and 180 parts of water;

[0217] The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra.

[0218] The preparation method of the Chinese medicine comprises the following steps:

[0219] (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture;

[0220] (2) adding water to the Chinese herbal mixture, soaking for 30 minutes, extracting at 98° C. for 60 minutes, and filtering to obtain a first filtrate and a medicinal residue; the mass volume ratio of the Chinese herbal mixture to water is 1 g:10 mL;

[0221] (3) adding water to the medicinal residue obtained in step (2), extracting at 98° C. for 40 min, filtering to obtain a second filtrate for standby use; the mass volume ratio of the medicinal residue to water is 1 g:6 mL;

[0222] (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.20, which is a traditional Chinese medicine.

[0223] The preparation method of the coltsfoot flower polysaccharide comprises the following steps:

[0224] 1) drying and crushing coltsfoot flowers, adding a composite enzyme (4% by weight of coltsfoot pollen) and water (55 times the weight of the coltsfoot pollen), stirring evenly, performing enzymolysis for 45 minutes, and then inactivating the enzyme to obtain an enzymolyzate; the composite enzyme comprises a neutral protease and a cellulase in an equal weight ratio;

[0225] 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 30 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution; the ultrasonic extraction power is 180W and the temperature is 40°C;

[0226] 3) adding 5 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), performing alcohol precipitation at 3° C. for 25 h, and then centrifuging, removing the supernatant and obtaining the precipitate, and freeze-drying the precipitate to obtain coltsfoot flower polysaccharide.

[0227] The preparation method of Qingfei granules comprises the following steps:

[0228] S1. Mix the Chinese medicinal agent, hesperidin, coltsfoot flower polysaccharide and water, heat to 65° C. while stirring, and then cool to obtain a premix for later use;

[0229] S2. Rubescensine A is first added to the premix obtained in step S1 and stirred, and then maltodextrin is added and stirred to obtain Qingfei granules.

[0230] Experimental Example 1 Acute toxicity test

[0231] 1.1 Test Method

[0232] 130 ICR mice, half male and half female, were randomly divided into 13 groups of 10 mice each. Qingfei granules prepared in Examples 1-3 and Comparative Examples 1-10 were prepared into a 0.8g / ml solution, the maximum concentration for mice, to ensure that each solution could pass through a 16-gauge mouse gavage needle. All groups of mice were fasted for 18 hours prior to the experiment and administered a 0.5ml / 10g solution. Immediately after administration, the animals were observed and recorded for symptoms of poisoning (e.g., mental state, motor function, etc.) and mortality. The observation period was 14 days.

[0233] 1.2 Test Results

[0234] The results are shown in Table 1:

[0235] Table 1 Acute toxicity determination

[0236]

[0237]

[0238] As shown in Table 1, the Qingfei granules prepared in the present application are safe and non-toxic and do not affect the growth of mice.

[0239] Experimental Example 2 Detection of inflammatory factors in mouse serum

[0240] 2.1 Detection method

[0241] A total of 150 ICR mice were randomly divided into 15 groups, with 10 mice in each group. 140 of these mice were treated with 20 mg / kg of PM2.5 inhalation exposure (PM2.5 particles were dissolved in sterile saline, mixed, and evenly mixed to a 0.02 mg / μL PM2.5 suspension, which was then administered intranasally using a pipette, with a volume of 20 μL per mouse). Ten other mice were treated with the same dose of saline for 1 hour. The saline-treated mice served as a blank control group, while the remaining PM2.5 inhalation-exposed mice were divided into a model control group, experimental groups 1-3, and comparative groups 1-10. On the day of modeling, mice in the model control group, experimental groups 1-3, and comparative groups 1-10 were gavaged with 100 mg / kg of 0.8 g / ml Qingfei Granule solution. The blank and model control groups were gavaged with an equal volume of saline. All mice received a normal diet and water intake. The animal experiment lasted 14 days. After the experiment, the mice were fasted for 10 hours but not water, and their eyeballs were removed to collect blood. Serum was obtained by centrifugation and the average concentration of inflammatory factors in the serum was detected using ELISA kits.

[0242] 2.2 Test results

[0243] As shown in Table 2:

[0244] Table 2 Contents of inflammatory factors in mouse serum

[0245]

[0246]

[0247] As can be seen from Table 2, compared with the model control group, the serum inflammatory factor content of mice in Experimental Examples 1 to 3 and Comparative Examples 1 to 10 groups was reduced, and compared with the blank control group, the serum inflammatory factor content of mice in Experimental Examples 1 to 3 and Comparative Examples 1 to 10 groups was increased, indicating that the Qingfei granules prepared in this application have the effect of inhibiting the inflammation caused by PM2.5 particles, especially Example 3 has the best inhibitory effect. Comparison of Example 3 with Comparative Examples 1 and 2 shows that the number of components affects the inhibitory effect of the final Qingfei granules on inflammation; Comparison of Example 3 with Comparative Examples 3 to 10 shows that the Qingfei granules contain hesperidin, coltsfoot polysaccharide, oridonin and breviscapine, which can improve the inhibitory effect on inflammation, among which hesperidin can reduce the release of inflammatory mediators and enhance antioxidant capacity, coltsfoot polysaccharide can activate immune cells (such as T lymphocytes and phagocytes), enhance the body's ability to regulate inflammatory responses, and the polyphenol components it contains can scavenge free radicals and reduce the damage of oxidative stress to lung tissue, oridonin can reduce the secretion of chemokines, reduce the level of oxidative stress and reduce ROS generation, breviscapine can scavenge free radicals and enhance the activity of endogenous antioxidant enzymes such as superoxide dismutase (SOD), and the above four components achieve the effect of reducing inflammation by exerting their own active substances.

[0248] Experimental Example 3 Antiasthma Test

[0249] 3.1 Test Method

[0250] Guinea pigs sensitive to wheezing (within two minutes), weighing 150-200g each, were randomly divided into four groups, each consisting of 10 animals. The four groups were experimental groups 1-3 (administered the Qingfei Granules prepared in Examples 1-3, dissolved in a 0.8g / ml Qingfei Granule solution) and a blank control group (administered normal saline). The animals were administered orally once daily at a dose of 3g / kg for three consecutive days. One hour after the last dose, the animals in each group were placed in a glass fogging chamber to observe the incubation period of wheezing in the guinea pigs.

[0251] 3.2 Test Results

[0252] As shown in Table 3:

[0253] Table 3 Antiasthmatic effect

[0254] Group Predrug latency (s) Post-drug latency period (s) Example 1 group 36±14 294±73 Example 2 group 34±10 285±61 Example 3 group 34±15 313±86 Blank control group 32±12 —

[0255] As can be seen from Table 3, taking the Qingfei granules of Examples 1 to 3 of the present application can prolong the latent period of asthma induction and has an antiasthmatic effect.

[0256] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A lung-clearing granule, characterized in that: The Qingfei granules include the following ingredients by weight: 30 to 50 parts of traditional Chinese medicine, 10 to 15 parts of hesperidin, 5 to 10 parts of coltsfoot polysaccharide, 2 to 6 parts of oridonin, 1 to 3 parts of breviscapine, 1 to 3 parts of maltodextrin and 100 to 200 parts of water; The Chinese medicine comprises the following ingredients by weight: 10 to 15 parts of jujube, 10 to 15 parts of scutellaria, 5 to 10 parts of platycodon, 5 to 10 parts of poria, 5 to 10 parts of tangerine peel, 5 to 10 parts of thunbergia bulb, 5 to 10 parts of mulberry bark, 3 to 8 parts of angelica, 3 to 8 parts of asparagus, 3 to 8 parts of gardenia, 3 to 8 parts of stewed bitter almonds, 3 to 8 parts of ophiopogon, 3 to 8 parts of ginger, 2 to 6 parts of liquorice and 1 to 4 parts of schisandra.

2. The Qingfei granules according to claim 1, characterized in that The Qingfei granules include the following ingredients by weight: 45 parts of traditional Chinese medicine, 12 parts of hesperidin, 9 parts of coltsfoot polysaccharide, 5 parts of oridonin, 2 parts of breviscapine, 2 parts of maltodextrin and 180 parts of water; The Chinese medicine comprises the following ingredients by weight: 12.5 parts of jujube, 12 parts of scutellaria, 8 parts of platycodon, 8 parts of poria, 8 parts of tangerine peel, 8 parts of thunbergia bulb, 8 parts of mulberry bark, 5.5 parts of angelica, 5.5 parts of asparagus, 5.5 parts of gardenia, 5.5 parts of stewed bitter almond, 5.5 parts of ophiopogon, 4 parts of ginger, 2.5 parts of liquorice and 2 parts of schisandra.

3. The Qingfei granules according to claim 1, characterized in that The preparation method of the Chinese medicine comprises the following steps: (1) mixing jujube, scutellaria, platycodon, poria, tangerine peel, thunbergia thunbergii, mulberry bark, angelica, asparagus cochinchinensis, gardenia jasminoides, boiled bitter almond, ophiopogon japonicus, ginger, liquorice and schisandra chinensis to obtain a Chinese medicine mixture; (2) adding water to the Chinese medicine mixture, soaking for 25 to 45 minutes, extracting at a temperature of 95 to 100° C. for 50 to 80 minutes, and filtering to obtain a first filtrate and medicinal residues; (3) adding water to the medicinal residue obtained in step (2), extracting at a temperature of 95-100° C. for 30-50 min, filtering to obtain a second filtrate for later use; (4) The first filtrate obtained in step (2) and the second filtrate obtained in step (3) are mixed, and concentrated under reduced pressure by supercritical spray to obtain an extract with a relative density of 1.15 to 1.23, which is a traditional Chinese medicine.

4. The Qingfei granules according to claim 3, characterized in that The mass volume ratio of the traditional Chinese medicine mixture and water in step (2) is 1g: (8-15)mL.

5. The Qingfei granules according to claim 3, characterized in that The mass volume ratio of the medicinal residue and water in step (3) is 1g:(5-8)mL.

6. The Qingfei granules according to claim 1, characterized in that The preparation method of the coltsfoot flower polysaccharide comprises the following steps: 1) drying and crushing the coltsfoot flower, adding a complex enzyme of 2-5% by weight of the coltsfoot flower pollen and water 50-60 times by weight of the coltsfoot flower pollen, stirring evenly, performing enzymolysis for 40-50 minutes, and then inactivating the enzyme to obtain an enzymolyzate; 2) subjecting the enzymatic hydrolysate obtained in step 1) to ultrasonic extraction for 20 to 40 minutes, followed by filtration and centrifugation to obtain an ultrasonic solution; 3) adding 4 to 6 times the volume of anhydrous ethanol to the ultrasonic solution obtained in step 2), performing alcohol precipitation at 2 to 4° C. for 20 to 30 hours, and then centrifuging, removing the supernatant and obtaining the precipitate, and freeze-drying the precipitate to obtain coltsfoot flower polysaccharide.

7. The Qingfei granules according to claim 6, characterized in that The complex enzyme in step 1) is composed of neutral protease and cellulase in equal weight ratio.

8. The Qingfei granules according to claim 6, characterized in that The power of the ultrasonic extraction in step 2) is 150-200W and the temperature is 30-50°C.

9. The method for preparing Qingfei granules according to claim 1, characterized in that: The following steps are involved: S1. Mix the Chinese medicinal agent, hesperidin, coltsfoot flower polysaccharide and water, heat to 60-70° C. while stirring, and then cool to obtain a premix for later use; S2. Rubescensine A and breviscapine are first added to the premix obtained in step S1 and stirred and mixed, and then maltodextrin is added and stirred to obtain Qingfei granules.

10. Use of the Qingfei granules according to any one of claims 1 to 8 or the Qingfei granules prepared by the preparation method according to claim 9 in preparing products for promoting lung metabolism, reducing inflammatory factors or relieving asthma.

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