Plasma dose calibration method and device

Abstract

The invention provides a plasma dose calibration method and device, and the device comprises a biological reaction module which is used for carrying a biological sample and providing a stable processing environment, and guaranteeing that plasma energy is transmitted to the biological sample; the plasma generation module is connected with the biological reaction module and is used for generating plasmas to the biological reaction module, controlling a discharge mode and energy output according to the discharge parameters and monitoring physical characteristic data of the plasmas; the biological verification module is used for carrying out quantitative detection on biophysical reactions under different plasma release doses to obtain biological effect data; the control center is connected with the biological verification module and the plasma generation module and used for obtaining biological effect data and physical property data of the plasma so as to adjust discharge parameters of the plasma generation module and control the plasma dosage. A closed-loop control system of'parameter regulation and control-biological treatment-effect feedback 'is constructed.

Description

Technical Field

[0001] The present invention relates to the field of plasma technology, and in particular to a plasma dose calibration method and device. Background Art

[0002] Atmospheric pressure cold plasma (CAP), due to its remarkable nonequilibrium properties, has been widely applied in fields such as biomedicine, agriculture, and material modification. Plasma produces reactive oxygen / nitrogen species (RONS) and other physical energy carriers (such as ultraviolet light and electric fields), which can produce significant biophysical effects on biological samples. In recent years, significant progress has been made in research on the application of plasma in areas such as sterilization and disinfection, wound healing, and cancer treatment, laying the theoretical foundation for its practical application.

[0003] Despite the enormous potential of plasma technology in various fields, existing methods for plasma dose assessment still have significant limitations. Currently, most studies rely on physical parameters (such as discharge voltage, power, and treatment time) to define plasma dose, but these parameters cannot directly reflect the actual effect of plasma on biological samples. Furthermore, while dose assessment methods based on equivalent total oxidation potential (ETOP) have theoretical scientific value, their complexity (e.g., requiring precise measurement of active particle concentration and oxidation potential) makes them difficult to implement in practical applications.

[0004] Existing methods fail to fully consider the heterogeneity of biological samples (such as cell density and differences in culture medium composition) and the impact of environmental factors (such as temperature and humidity) on plasma dose, resulting in insufficient accuracy and repeatability of dose assessment. Summary of the Invention

[0005] The present invention provides a plasma dose calibration method and device to address the problem in the prior art that the heterogeneity of biological samples (such as cell density and differences in culture medium composition) and the impact of environmental factors (such as temperature and humidity) on plasma dose are not fully considered, resulting in insufficient accuracy and repeatability of dose assessment.

[0006] The present invention provides a plasma dose calibration device, comprising: A bioreactor module, configured to carry biological samples and provide a stable processing environment to ensure that plasma energy is transferred to the biological samples; a plasma generating module connected to the bioreactor module, for generating plasma into the bioreactor module, controlling the discharge mode and energy output according to discharge parameters, and monitoring the physical property data of the plasma; Biological verification module, used to quantitatively detect biophysical reactions under different plasma release doses and obtain biological effect data; The control center is connected to the biological verification module and the plasma generation module respectively, and is used to obtain the biological effect data and the physical characteristic data of the plasma to adjust the discharge parameters of the plasma generation module and control the plasma dose.

[0007] According to the plasma dose calibration device provided by the present invention, the control center predicts and adjusts the discharge parameters of the plasma generating module based on a preloaded dynamic mapping model of discharge parameters and biological effect data, and obtains the biological effect data and the physical property data of the plasma; The physical property data of the plasma include the active species concentration and energy flux of the plasma, and the active species include OH·, O3, and NOx; The biological effect data include: cell survival rate, gene expression level, and sterilization efficiency; The discharge parameters include at least one of: voltage, frequency, gas ratio and processing time.

[0008] According to the plasma dose calibration device provided by the present invention, the plasma generating module includes: a discharge unit for providing multiple discharge modes, including a dielectric barrier discharge mode, a corona discharge mode, a floating electrode discharge mode or a jet mode; A power control unit, used to control the current, voltage, and frequency of the high-voltage power supply to coordinately control the energy output of the plasma; the current range is 0.1-50 mA, the voltage range is 1-30 kV, and the frequency range is 50 Hz-100 kHz; The gas proportioning unit is used to proportion inert gas, reactive gas and their mixed gas.

[0009] According to the plasma dose calibration device provided by the present invention, the plasma generation module includes: a spectrometer, an electrical probe array and a microwave interferometer; The spectrometer is used to monitor the intensity of characteristic spectral lines of active particles in the plasma and calculate the concentration of active particles through spectral analysis; The electrical probe array is used to measure the electron density and energy distribution in the plasma and provide relevant data of the energy flux; The microwave interferometer is used to measure the electron density and temperature of the plasma to assist in calculating the energy flux.

[0010] According to the plasma dose calibration device provided by the present invention, the bioreaction module includes: Sealed reaction chamber, used to isolate external environmental interference; Environmental maintenance unit, used to control O2, CO2, humidity and temperature in the cabin; A mechanical positioning unit, including a multi-degree-of-freedom robotic arm or an electronically controlled platform, is used to align with the plasma action area; Safety protection unit, including ozone decomposition catalyst and negative pressure protection device.

[0011] According to the plasma dose calibration device provided by the present invention, the biological verification module includes: Microscopic imaging unit, including a motorized focusing fluorescence microscope for capturing changes in cell membrane integrity and organelle morphology; Biochemical analysis unit, including a multispectral CCD camera and a miniaturized biochemical sensor array for activity detection and medium component analysis; The data output interface is used to feed back the generated bio-effect data to the control center.

[0012] The present invention also provides a plasma dose calibration method, comprising: Generate plasma through the plasma generation module and send it to the bioreactor module to act on the untreated biological sample placed in the bioreactor module, and control the discharge mode and energy output according to the discharge parameters, and monitor the physical property data of the plasma; The biophysical response under different plasma release doses is quantitatively detected through the biological verification module to obtain biological effect data; The biological effect data and the physical characteristic data of the plasma are acquired through a control center to adjust the discharge parameters of the plasma generating module and control the plasma dose.

[0013] According to the plasma dose calibration method provided by the present invention, the step of generating plasma by the plasma generating module specifically includes: Selecting a suitable discharge method according to the physical state of the sample and the processing requirements, wherein the discharge method includes dielectric barrier discharge, corona discharge, floating electrode discharge or jet discharge; The energy output of the plasma is coordinated and regulated through the current, voltage and frequency of the high-voltage power supply; The chemical properties of the plasma are optimized by matching the inert gas, reactive gas and their mixture.

[0014] According to the plasma dose calibration method provided by the present invention, the biophysical reactions under different plasma release doses are quantitatively detected by the biological verification module to obtain biological effect data, which specifically includes: Capture changes in cell membrane integrity and organelle morphology through microscopic imaging units; Activity detection and medium component analysis are performed through the biochemical analysis unit; The generated bioeffect data is fed back to the control center through the data output interface.

[0015] According to the plasma dose calibration method provided by the present invention, the biological effect data and the physical property data of the plasma are obtained by a control center to adjust the discharge parameters of the plasma generating module, which specifically includes: Real-time collection of plasma physical property data and biological effect data; The discharge parameters of the plasma generating module are predicted and adjusted by using a dynamic mapping model between discharge parameters and bio-effect data established based on a reinforcement learning algorithm, the bio-effect data and the physical property data of the plasma.

[0016] The plasma dose calibration method and device provided by the present invention accurately control the discharge parameters and monitor the physical property data of the plasma through the plasma generation module, and the biological verification module quantitatively detects the processed samples to obtain biological effect data. The control center combines the two types of data and dynamically adjusts the discharge parameters based on the reinforcement learning algorithm, thereby achieving precise control of the plasma dose. By constructing a closed-loop control system of "parameter regulation-biological treatment-effect feedback", it solves the problem in the existing technology that the heterogeneity of biological samples and environmental factors are not fully considered, resulting in inaccurate and non-repeatable dose assessment. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the present invention or the prior art, a brief introduction will be given below to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0018] Figure 1 It is a structural schematic diagram of the plasma dose calibration device provided by the present invention.

[0019] Figure 2 It is a flow chart of the plasma dose calibration method provided by the present invention. DETAILED DESCRIPTION

[0020] To make the objectives, technical solutions, and advantages of the present invention more clear, the technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0021] First, the terms involved in the embodiments of the present invention are schematically explained.

[0022] Plasma: Plasma is a state of matter composed of particles such as ions, electrons, atoms, and molecules. It is electrically neutral but conductive. Due to its non-equilibrium properties, plasma is widely used in biomedicine, agriculture, and materials modification.

[0023] Spectrometer: An instrument used to measure properties of light, such as wavelength and intensity. In plasma monitoring, a spectrometer analyzes the spectrum emitted by a plasma to determine the type and concentration of active species.

[0024] Discharge parameters: refers to the parameters that control the operation of the plasma generator, including voltage, current, frequency, gas ratio, processing time, etc. These parameters directly affect the characteristics and energy output of the plasma.

[0025] Biological effect data: refers to the response data of biological samples after plasma treatment, including cell survival rate, gene expression, metabolic substance changes, sterilization efficiency, etc., which are used to evaluate the biophysical effects of plasma.

[0026] Reactive species: These are particles with high reactivity in plasma, such as reactive oxygen species (OH·, O3) and reactive nitrogen species (NOx). These particles play a key role in the interaction between plasma and biological samples.

[0027] Energy flux: refers to the energy passing through a unit area per unit time. It is usually used to describe the energy output intensity of a plasma and is measured in J / cm².

[0028] Cell viability: refers to the proportion of cells that remain active after plasma treatment, and is an important indicator for evaluating the biological effects of plasma.

[0029] Gene expression: This refers to the expression level of a specific gene in a cell, typically assessed by measuring mRNA or protein levels. Changes in gene expression can reflect the effects of plasma on cellular function.

[0030] Sterilization efficiency: refers to the ability of plasma to kill microorganisms (such as bacteria and viruses), usually expressed as the ratio of reduction in the number of microorganisms before and after treatment.

[0031] In biomedical research, plasma technology is widely used in fields such as cell processing, sterilization and disinfection, and tissue repair. However, existing technologies have significant limitations in plasma dose assessment. On the one hand, the discretization of equipment requires frequent transfer of samples between different devices, which not only increases the complexity of operation, but may also introduce contamination and mechanical damage, especially when dealing with delicate structures such as 3D organoids. On the other hand, dose control strategies usually rely on preset physical parameters (such as voltage, frequency, time, etc.) and lack accurate feedback on the actual biological effect dose. This open-loop control mode makes it difficult to repeat the treatment effect, especially under different experimental conditions. In addition, the existing technology has insufficient control over environmental factors (such as temperature and humidity), resulting in an unstable processing environment, which further affects the reliability of experimental data.

[0032] In order to solve the above problems, the present application proposes a plasma dose calibration method and device. This method realizes the precise control of plasma dose by constructing a closed-loop control system of "parameter control-biological treatment-effect feedback". Specifically, the plasma generation module can accurately control the discharge parameters (such as voltage, frequency, gas ratio, treatment time) and monitor the physical property data of the plasma (such as active particle concentration, energy flux). The biological verification module performs quantitative detection on the treated biological samples to obtain biological effect data (such as cell survival rate, gene expression level, sterilization efficiency). The control center combines these two types of data and uses the reinforcement learning algorithm to dynamically adjust the discharge parameters to ensure the accuracy of the plasma dose and the stability of the treatment effect. In addition, the biological reaction module provides a stable processing environment to ensure that the sample is not disturbed by the outside world during the processing process.

[0033] Through this innovative design, the embodiments of the present invention significantly improve the accuracy and repeatability of plasma dose assessment, providing scientific support for the application of plasma technology in fields such as biomedicine.

[0034] Specifically, see Figure 1 , the plasma dose calibration device of an embodiment of the present invention includes: The device achieves precise control of plasma dose and stable evaluation of biological effects through the coordinated work of plasma generation module, biological reaction module, biological verification module and control center.

[0035] The plasma generation module is the core of the entire device, responsible for generating the plasma and precisely controlling discharge parameters. It supports multiple discharge modes, including dielectric barrier discharge, corona discharge, floating electrode discharge, and jet discharge. Researchers can select the most appropriate discharge mode based on the sample's physical state and processing requirements. For example, dielectric barrier discharge is suitable for flat samples requiring uniform processing, while jet discharge is ideal for localized, precise processing. The power control unit precisely regulates the current (0.1-50 mA), voltage (1-30 kV), and frequency (50 Hz-100 kHz) of the high-voltage power supply to optimize the plasma's energy output. The gas proportioning unit precisely adjusts the composition of inert gases (such as He and Ar) and reactive gases (such as N₂ and O₂), as well as their mixtures, to tailor the plasma's chemical properties. Furthermore, the plasma generation module integrates monitoring instruments such as a spectrometer, an electrical probe array, and a microwave interferometer to provide real-time monitoring of plasma physical properties, such as reactive species concentrations (OH·, O₃, NOx) and energy flux (0.1-10 J / cm²), providing crucial information for subsequent dose control.

[0036] The bioreactor module provides a stable processing environment for biological samples, ensuring efficient delivery of plasma energy to the samples. It features a sealed reaction chamber design with a built-in adapter rack for multiple formats, compatible with standard 96-well, 16-well, and 6-well plates, as well as customized culture vessels (10-100mm diameter). This supports the processing of cells, bacteria, fungi, viruses, and other organisms, including their culture media, while effectively preventing interference from the external environment. The environmental maintenance unit precisely controls the chamber's O2 (1-21%), CO2 (0-20%), humidity (30-95% RH), and temperature (4-45°C ± 0.1°C), ensuring ideal sample conditions during processing. The mechanical positioning unit, comprised of a multi-degree-of-freedom robotic arm or electronically controlled stage, precisely targets the plasma action zone, ensuring uniform plasma energy delivery to the sample. The safety protection unit, equipped with an ozone decomposition catalyst and a negative pressure protection device, effectively prevents the accumulation of ozone and other byproducts, ensuring operational safety.

[0037] The bioverification module is responsible for quantitatively monitoring biophysical responses to different plasma doses. The motorized focusing fluorescence microscope in the microscopic imaging unit captures microscopic features such as cell membrane integrity and changes in organelle morphology, providing researchers with intuitive information on cell morphological changes. The biochemical analysis unit, which includes a multispectral CCD camera and a miniaturized biochemical sensor array, is capable of performing activity assays (such as the MTT assay and calcein / PI double staining) and medium composition analysis (pH, conductivity, and ROS / RNS concentrations). These analytical methods provide quantitative data for evaluating the biophysical effects of the plasma. A data output interface feeds the bioverification module's test results back to the control center, forming a closed-loop control system. This real-time feedback mechanism enables the device to adjust plasma discharge parameters based on bioeffect data, thereby achieving precise dose control.

[0038] The control center is the intelligent core of the device, responsible for data processing and dynamic adjustment of discharge parameters. It uses the data acquisition unit to collect real-time data on the plasma's physical properties and bioeffects. It then establishes a mapping relationship between discharge parameters and bioeffects using a dynamic mapping model based on a reinforcement learning algorithm. The feedback optimization unit dynamically adjusts discharge parameters based on real-time monitoring data to ensure precise control of plasma dose and stable treatment results. The database unit stores the media characteristics, discharge parameters, and bioeffects data for each treatment, providing data support for subsequent intelligent parameter recommendations.

[0039] The plasma generation module and the bioreactor module are physically connected to achieve plasma transfer. The plasma generated by the plasma generation module is transferred to the sealed reaction chamber of the bioreactor module via a specially designed interface or pipeline, ensuring that the plasma acts evenly on the biological sample. This connection method not only ensures effective plasma transfer but also prevents the external environment from affecting the plasma's properties.

[0040] A connection exists between the bio-verification module and the bio-reactor module for sample transfer and data feedback. After plasma treatment, the biological sample is processed in the bio-reactor module and then transferred to the bio-verification module for testing via a mechanical positioning unit or sample transfer device. The test results from the bio-verification module are fed back to the control center in real time via a data output interface, forming a closed-loop control system. This connection ensures that the processed sample can be tested promptly and the test results can be quickly used to optimize subsequent processing.

[0041] The control center is connected to the plasma generation module and the bio-verification module via data cables or wireless communications. The control center collects real-time monitoring data from the plasma generation module and test results from the bio-verification module, adjusting the plasma generation module's discharge parameters using a dynamic mapping model and feedback optimization unit. This data connection ensures that the control center can dynamically adjust the plasma generation and processing process based on real-time data, achieving precise dose control.

[0042] Through this innovative design, the plasma dose calibration device of the embodiment of the present invention significantly improves the accuracy and repeatability of plasma dose assessment, and provides scientific support for the application of plasma technology in biomedicine and other fields.

[0043] During the specific use phase, the operator first sets the target biological effect type on the controller, such as cell inactivation, proliferation, seed activation, or material modification. The system then automatically recommends an initial parameter combination for the plasma generation module based on a built-in database, including discharge mode, voltage, frequency, gas ratio, and treatment time. The plasma generation module generates plasma based on these parameters and collects real-time data such as active particle concentration and energy flux through a monitoring interface to ensure the rationality of the initial settings.

[0044] At the same time, the bioreactor module activates its environmental maintenance system, precisely controlling the chamber's oxygen, carbon dioxide, humidity, and temperature to provide a stable processing environment for the samples. A mechanical positioning unit precisely aligns the culture container with the plasma action zone, ensuring uniform plasma energy delivery to the samples. A safety protection unit activates to prevent the accumulation of byproducts such as ozone, ensuring safe operation.

[0045] An untreated biological sample is placed in the sealed reaction chamber of the bioreactor module, and the environmental maintenance system is activated to ensure a stable processing environment. After the target biological effect type is set on the control center, the system automatically recommends an initial parameter combination for the plasma generation module. Plasma treatment then begins.

[0046] The plasma generation module generates plasma according to preset discharge patterns and parameters. The discharge mode is selected based on the sample type and experimental requirements, such as dielectric barrier discharge for flat samples and jet mode for localized treatment. The power control unit coordinates current, voltage, and frequency to optimize plasma energy output. The gas proportioning unit precisely mixes inert and reactive gases to further adjust the plasma's chemical properties. The monitoring interface provides real-time monitoring of plasma physical properties, such as active species concentration and energy flux, to ensure accurate plasma dosing.

[0047] After the plasma treatment is complete, the bio-verification module tests the sample. The microscopic imaging unit and biochemical analysis unit evaluate the treatment effect from a morphological and biochemical perspective, respectively, and feed the data back to the control center.

[0048] The control center collects real-time data on the plasma's physical properties and biological effect data from the bio-verification module. Based on a dynamic mapping model using a reinforcement learning algorithm, the control center establishes a mapping relationship between discharge parameters and biological effects. The feedback optimization unit dynamically adjusts discharge parameters based on real-time monitoring data, such as increasing discharge power or extending treatment time, to ensure precise control of plasma dose and stable treatment results. The database unit stores detailed data from each treatment, providing a reference and optimization basis for subsequent processing.

[0049] The plasma dose calibration method provided by the present invention is described below. The plasma dose calibration device described below and the plasma dose calibration method described above can be referred to in correspondence with each other.

[0050] Figure 2 This is one of the flow charts of the plasma dose calibration method provided by the present invention, such as Figure 2 As shown, the method includes the following: Step 201: Generate plasma from a plasma generating module to a bioreactor module to act on an untreated biological sample placed in the bioreactor module, control the discharge mode and energy output according to discharge parameters, and monitor the physical property data of the plasma.

[0051] The plasma generation module generates plasma according to the set discharge parameters and transfers it to the bioreactor module. Specifically, the operator first selects the appropriate discharge mode, such as dielectric barrier discharge, corona discharge, floating electrode discharge, or jet mode, through the control center based on the physical state of the sample and the processing requirements. The plasma generation module uses the power control unit to precisely set the current (0.1-50 mA), voltage (1-30 kV), and frequency (50 Hz-100 kHz) of the high-voltage power supply to optimize the energy output of the plasma. The gas proportioning unit accurately proportions inert gases (such as He, Ar) and reactive gases (such as N2, O2) and their mixtures according to experimental requirements to further adjust the chemical properties of the plasma. The generated plasma is transferred to the closed reaction chamber of the bioreactor module through specially designed interfaces or pipelines to act on untreated biological samples. During this process, the plasma generation module uses monitoring instruments such as spectrometers, electrical probe arrays, and microwave interferometers to monitor the physical properties of the plasma in real time, such as active particle concentrations (OH·, O3, NOx) and energy flux (0.1-10 J / cm²), to ensure the accuracy of the plasma dose.

[0052] Step 202: Quantitatively detect the biophysical reactions under different plasma release doses through a biological verification module to obtain biological effect data.

[0053] In step 202, the bioverification module quantitatively measures biophysical responses to different plasma doses. The motorized focusing fluorescence microscope in the microscopic imaging unit captures microscopic features such as cell membrane integrity and changes in organelle morphology, providing researchers with intuitive information on cell morphological changes. The biochemical analysis unit utilizes a multispectral CCD camera and a miniaturized biochemical sensor array to perform activity assays (such as the MTT assay and calcein / PI double staining) and medium composition analysis (pH, conductivity, and ROS / RNS concentrations). These analytical methods assess the biophysical effects of the plasma from a biochemical perspective, and the test results are fed back to the control center via a data output interface, forming a closed-loop control system. The test results from the bioverification module not only provide direct evidence of the plasma treatment effect but also provide an important basis for subsequent dose optimization.

[0054] Step 203: Acquire the biological effect data and the physical property data of the plasma through a control center to adjust the discharge parameters of the plasma generating module and control the plasma dose.

[0055] In step 203, the control center obtains the physical property data and biological effect data of the plasma in real time through the data acquisition unit. Based on the dynamic mapping model of the reinforcement learning algorithm, the control center establishes a mapping relationship between the discharge parameters and the biological effects. The feedback optimization unit dynamically adjusts the discharge parameters according to the real-time monitoring data, such as increasing the discharge power or extending the treatment time, to ensure the precise control of the plasma dose and the stability of the treatment effect. The database unit stores detailed data of each treatment, including medium characteristics, discharge parameters and biological effect data, to provide a reference and optimization basis for subsequent treatment. Through this closed-loop control and real-time feedback optimization, the device significantly improves the accuracy and repeatability of plasma dose assessment, and provides scientific support for the application of plasma technology in biomedicine and other fields.

[0056] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A plasma dose calibration device, characterized in that: include: A bioreactor module, configured to carry biological samples and provide a stable processing environment to ensure that plasma energy is transferred to the biological samples; a plasma generating module connected to the bioreactor module, for generating plasma into the bioreactor module, controlling the discharge mode and energy output according to discharge parameters, and monitoring the physical property data of the plasma; Biological verification module, used to quantitatively detect biophysical reactions under different plasma release doses and obtain biological effect data; The control center is connected to the biological verification module and the plasma generation module respectively, and is used to obtain the biological effect data and the physical characteristic data of the plasma to adjust the discharge parameters of the plasma generation module and control the plasma dose.

2. The plasma dose calibration device according to claim 1, characterized in that: The control center predicts and adjusts the discharge parameters of the plasma generation module based on a preloaded dynamic mapping model of discharge parameters and bio-effect data, and obtains the bio-effect data and the physical property data of the plasma; The physical property data of the plasma include the active species concentration and energy flux of the plasma, and the active species include OH·, O3, and NOx; The biological effect data include: cell survival rate, gene expression level, and sterilization efficiency; The discharge parameters include at least one of: voltage, frequency, gas ratio and processing time.

3. The plasma dose calibration device according to claim 1, characterized in that: The plasma generating module comprises: A discharge unit, configured to provide a variety of discharge modes, including dielectric barrier discharge, corona discharge, floating electrode discharge, or jet discharge; A power control unit, used to control the current, voltage, and frequency of the high-voltage power supply to coordinately control the energy output of the plasma; the current range is 0.1-50 mA, the voltage range is 1-30 kV, and the frequency range is 50 Hz-100 kHz; The gas proportioning unit is used to proportion inert gas, reactive gas and their mixed gas.

4. The plasma dose calibration device according to claim 1 or 3, characterized in that: The plasma generation module includes: a spectrometer, an electrical probe array and a microwave interferometer; The spectrometer is used to monitor the intensity of characteristic spectral lines of active particles in the plasma and calculate the concentration of active particles through spectral analysis; The electrical probe array is used to measure the electron density and energy distribution in the plasma and provide relevant data of the energy flux; The microwave interferometer is used to measure the electron density and temperature of the plasma to assist in calculating the energy flux.

5. The plasma dose calibration device according to claim 1, characterized in that: The bioreactor module comprises: Sealed reaction chamber, used to isolate external environmental interference; Environmental maintenance unit, used to control O2, CO2, humidity and temperature in the cabin; A mechanical positioning unit, including a multi-degree-of-freedom robotic arm or an electronically controlled platform, is used to align with the plasma action area; Safety protection unit, including ozone decomposition catalyst and negative pressure protection device.

6. The plasma dose calibration device according to claim 1, characterized in that: The biometric verification module includes: Microscopic imaging unit, including a motorized focusing fluorescence microscope for capturing changes in cell membrane integrity and organelle morphology; Biochemical analysis unit, including a multispectral CCD camera and a miniaturized biochemical sensor array for activity detection and medium component analysis; The data output interface is used to feed back the generated bio-effect data to the control center.

7. A plasma dose calibration method, characterized in that: include: Generate plasma from the plasma generating module to the bioreactor module, act on an untreated biological sample placed in the bioreactor module, control the discharge mode and energy output according to discharge parameters, and monitor physical property data of the plasma; The biophysical response under different plasma release doses is quantitatively detected through the biological verification module to obtain biological effect data; The biological effect data and the physical characteristic data of the plasma are acquired through a control center to adjust the discharge parameters of the plasma generating module and control the plasma dose.

8. The plasma dose calibration method according to claim 7, characterized in that: The step of generating plasma by the plasma generating module specifically includes: Selecting a suitable discharge method according to the physical state of the sample and the processing requirements, wherein the discharge method includes dielectric barrier discharge, corona discharge, floating electrode discharge or jet discharge; The energy output of the plasma is coordinated and regulated through the current, voltage and frequency of the high-voltage power supply; The chemical properties of the plasma are optimized by matching the inert gas, reactive gas and their mixture.

9. The plasma dose calibration method according to claim 7, characterized in that: The bio-calibration module quantitatively detects biophysical reactions under different plasma release doses to obtain biological effect data, including: Capture changes in cell membrane integrity and organelle morphology through microscopic imaging units; Activity detection and medium component analysis are performed through the biochemical analysis unit; The generated bioeffect data is fed back to the control center through the data output interface.

10. The plasma dose calibration method according to claim 7, characterized in that: The biological effect data and the physical property data of the plasma are acquired through a control center to adjust the discharge parameters of the plasma generation module, specifically including: Real-time collection of plasma physical property data and biological effect data; The discharge parameters of the plasma generating module are predicted and adjusted by using a dynamic mapping model between discharge parameters and bio-effect data established based on a reinforcement learning algorithm, the bio-effect data and the physical property data of the plasma.

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