Pre-assembly structure, pre-assembly method and implantation method of microwire electrodes

Abstract

This application discloses a pre-assembled structure, pre-assembled method, and implantation method for a microfilament electrode. The pre-assembled structure includes: a microfilament electrode having a substrate and multiple microfilament structures attached to the substrate; an organic thin film with the microfilament structures arranged on it; and a rigid implantation tool fixedly connected to the microfilament structures. The rigid implantation tool is used to implant the electrode recording sites on the microfilament structures into a target object. The technical solution of this application effectively solves the problems in the prior art where microfilament electrodes easily adhere to the substrate during drying, leading to separation difficulties, and where dried microfilament electrodes are unstable in air, easily drifting and entangled, causing operational difficulties in subsequent implantation steps.

Description

Technical Field

[0001] This application relates to the field of electrode technology, and more specifically, to a pre-assembled structure, pre-assembled method, and implantation method for a microwire electrode. Background Technology

[0002] In the biomedical field, flexible microfilament electrodes have attracted considerable attention due to their excellent biocompatibility and high-throughput neural signal acquisition performance. The fabrication of flexible microfilament electrodes typically involves micro / nano fabrication techniques, where electrode microfilaments are shaped and fabricated on a silicon substrate. However, existing microfilament electrode fabrication and implantation techniques face several key challenges that limit their widespread application in neuroscience and clinical settings.

[0003] First, traditional drying methods for microfilament electrodes, such as critical point drying, can effectively separate flexible microfilament electrodes from silicon substrates. However, due to the lack of stability of the electrode microfilaments in air, they are prone to large-scale swaying and even tangling. This not only increases the complexity of subsequent pre-assembly operations but may also damage the microfilaments, affecting implantation results and electrode performance.

[0004] Secondly, when using current implantation methods for microfilament electrode implantation, especially when multiple electrodes need to be implanted at different depths to cover specific neural networks or brain regions, the implantation depth of each electrode microfilament cannot be independently controlled, making it difficult to accurately implant multiple microfilament electrodes.

[0005] Furthermore, while existing locating layer designs are used to fix electrode wires, their fabrication requires additional micro / nano fabrication steps, increasing process complexity and cost. Simultaneously, the surface morphology of the locating layer in contact with the electrode wire is difficult to control, affecting the adhesion performance of the electrode wire and the reliability of subsequent operations. The selection of locating layer materials is also limited by the compatibility with micro / nano fabrication, restricting the diversity of its performance and functions.

[0006] In summary, existing technologies have shortcomings in the drying of flexible microwire electrodes and the precise control of implantation depth. A more stable, efficient, and controllable drying and implantation method is needed to overcome these technical deficiencies. Summary of the Invention

[0007] This application provides a pre-assembled structure, pre-assembled method, and implantation method for a microfilament electrode to solve the problem in related technologies where the drying process of flexible microfilament electrodes easily leads to damage to the microfilaments, thereby affecting the implantation effect and electrode performance.

[0008] According to one aspect of this application, a pre-assembled structure of a microfilament electrode is provided, comprising: a microfilament electrode having a substrate and a plurality of microfilament structures attached to the substrate; an organic thin film having the microfilament structures arranged thereon; and a rigid implantation tool fixedly connected to the microfilament structures, the rigid implantation tool being used to implant electrode recording sites on the microfilament structures into a target object.

[0009] Optionally, the material of the organic film includes at least one selected from parylene, polyimide, polydimethylsiloxane, polytetrafluoroethylene, polyethylene terephthalate, polyethylene, and polypropylene.

[0010] Optionally, one side of the organic film has a surface microstructure: the surface microstructure is selected from at least one of a grid structure and a stripe structure, and the microfilament structure is in contact with at least a portion of the surface microstructure; or the surface microstructure includes a plurality of groove structures, with a plurality of microfilament structures embedded in the groove structures, and each microfilament structure is embedded in a different groove structure.

[0011] Optionally, the first surface of the organic film is hydrophobic, and the microfilament structure is in contact with at least a portion of the first surface.

[0012] Optionally, the pre-assembled structure further includes: an electrode housing having a plurality of receiving slots equal in number to the microfilament structures, and the electrode housing being fixedly connected to the substrate so that the microfilament structures correspond one-to-one with and are aligned with the receiving slots, and the second surface of the substrate of the microfilament electrode being parallel to the third surface of the electrode housing, wherein the second surface is the side surface of the substrate on which the microfilament structures are formed, and the third surface is the side surface of the electrode housing on which the receiving slots are formed; the rigid implantation tool is a plurality of implantation needles equal in number to the microfilament structures, the end of the implantation needle being fixedly connected to the end of each microfilament structure, and the implantation needles being disposed one-to-one in the receiving slots.

[0013] Optionally, the pre-assembled structure further includes a support tube, in which the implantation needle is partially disposed, and the end of the implantation needle is located outside the support tube, and the support tube containing the implantation needle is disposed in the receiving groove.

[0014] Optionally, the plurality of receiving grooves of the electrode housing are tubular through holes, the implantation needle is partially disposed in the through holes, and the end of the implantation needle is located outside the through holes.

[0015] According to another aspect of this application, a pre-assembly method for a microfilament electrode is provided, comprising the following steps: providing a microfilament electrode having a substrate and multiple microfilament structures attached to the substrate; placing the microfilament electrode in a container containing an organic solvent for drying, wherein an organic film is disposed inside the container, and the dried microfilament structures are arranged on the organic film; and pre-assembling the microfilament structures of the microfilament electrode with a rigid implantation tool to fix the microfilament structures to the rigid implantation tool, thereby obtaining the pre-assembled structure of the microfilament electrode described above.

[0016] Optionally, during the process of placing the microfilament electrode in the container, the step of changing the microfilament structure from an attached state to a separated state, so that the microfilament structure is arranged on the organic film, includes: dicing the substrate to remove a first target region in the substrate, wherein the first target region includes at least a portion of the substrate in the attached state where the microfilament structure is attached; and disposing at least a portion of the organic film between the microfilament structure and the substrate so that the dried multiple microfilament structures are arranged on the organic film.

[0017] Optionally, the step of arranging multiple microfilament structures on the organic film further includes: after the step of disposing at least a portion of the organic film between the microfilament structure and the substrate, aspirating the organic solvent in a second target region, wherein the second target region is a local area in the container having the microfilament structure.

[0018] Optionally, the rigid implantation tool comprises multiple implantation needles, the same number as the microfilament structures. The step of pre-assembling the microfilament structures with the rigid implantation tool includes: providing an electrode housing having multiple receiving slots, the same number as the microfilament structures; fixing the electrode housing to the substrate so that the microfilament structures correspond one-to-one with and are aligned with the receiving slots, and the second surface of the substrate of the microfilament electrode is parallel to the third surface of the electrode housing, wherein the second surface is the side surface of the substrate on which the microfilament structures are formed, and the third surface is the side surface of the electrode housing on which the receiving slots are formed; fixing the end of the implantation needle to the end of each microfilament structure, and positioning the implantation needles one-to-one in the receiving slots.

[0019] Optionally, the step of pre-assembling the microfilament structure with the rigid implantation tool further includes: before the step of fixing the end of the implantation needle to the end of the microfilament structure, partially placing the implantation needle in the support tube, with the end of the implantation needle located outside the support tube; and in the step of placing the implantation needle in the receiving groove, placing the support tube containing the implantation needle in the receiving groove.

[0020] According to another aspect of this application, a method for implanting a microfilament electrode is also provided, comprising the following steps: providing a pre-assembled structure of the microfilament electrode as described above; using the rigid implantation tool to implant the electrode recording site of the microfilament structure into a target object, wherein during the implantation of the electrode recording site, the microfilament structure and the organic film change from an attached state to a separated state.

[0021] Optionally, each of the microfilament structures includes a connected stretching portion and an implantation end, with the electrode recording site located on the implantation end. The step of implanting the electrode recording site of the microfilament structure into the target object using the rigid implantation tool includes: placing the stretching portion in a stretched state under the action of the rigid implantation tool to implant the electrode recording site into the target object.

[0022] Optionally, the rigid implantation tool is a plurality of implantation needles, the same number as the number of microfilament structures. The step of implanting the electrode recording site into the target object using the rigid implantation tool includes: the rigid implantation tool is a plurality of implantation needles, the same number as the number of microfilament structures. The step of implanting the electrode recording site into the target object using the rigid implantation tool includes: using the fixing module to control the movement of the implantation needles to implant the electrode recording site into the target object at a target depth.

[0023] This application provides a pre-assembled structure for a microfilament electrode. The microfilament electrode has a substrate and multiple microfilament structures attached to the substrate. The pre-assembled structure also includes an organic film and a rigid implantation tool. The microfilament structures are arranged on the organic film, and the rigid implantation tool is fixedly connected to the microfilament structures for implanting the electrode recording sites on the microfilament structures into the target object. During the drying process of separating the flexible microfilament electrode from the silicon substrate, the organic film prevents the microfilament structures from drifting, tangling, and breaking after drying, ensuring the spatial stability of the microfilament structure and facilitating subsequent implantation steps. This improves the implantation effect and performance of the pre-assembled microfilament electrode. Furthermore, the pre-assembled rigid implantation tool significantly shortens the surgical implantation time, improves implantation efficiency, and reduces surgical difficulty and risk. Simultaneously, the implantation depth of each microfilament structure can be independently controlled, facilitating precise implantation into the target brain region and improving the accuracy and reliability of the experiment. Attached Figure Description

[0024] The accompanying drawings, which form part of this application, are used to provide a further understanding of this application. The illustrative embodiments and descriptions of this application are used to explain this application and do not constitute an undue limitation of this application. In the drawings:

[0025] Figure 1 This is a top view schematic diagram of a pre-assembled structure of a microwire electrode according to an embodiment of this application;

[0026] Figure 2 This is a top view schematic diagram of another pre-assembled structure of a microfilament electrode provided according to an embodiment of this application;

[0027] Figure 3 This is a process flow diagram of a pre-assembly method for a microwire electrode according to an embodiment of this application;

[0028] Figure 4 This is a top view schematic diagram of the microfilament electrode provided in a pre-assembly method for a microfilament electrode according to an embodiment of this application.

[0029] Figure 5 Yes Figure 4 The diagram shows a top view of the substrate after the microwire electrode substrate has been diced.

[0030] Figure 6 It is Figure 1 The diagram shows a top view of the substrate after the dried microfilament electrode has been fixedly connected to the electrode shell.

[0031] Figure 7 This is a process flow diagram of a microwire electrode implantation method provided according to an embodiment of this application;

[0032] Figure 8 This is a schematic diagram of a method for implanting a microfilament electrode according to an embodiment of this application, in which the end of the implantation needle is fixedly connected to the end of the microfilament structure.

[0033] Figure 9 It is control Figure 8 The diagram shows a schematic of the structure in which the implantation needle moves to implant the electrode recording site into the target object.

[0034] Figure 10 It is Figure 9 The diagram shows the structure after the implanted needle has been removed;

[0035] Figure 11 A schematic diagram of a structure that uses a fixing module to simultaneously fix the tail of multiple implantation needles is shown.

[0036] The above figures include the following reference numerals:

[0037] 10. Substrate; 20. Microfilament structure; 201. Implantation end; 202. Tensioning part; 30. Transport part; 40. Organic thin film; 50. Electrode shell; 60. Receiving groove; 70. Implantation needle; 80. Target object; 90. Fixation module. Detailed Implementation

[0038] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0039] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort should fall within the scope of protection of the present application.

[0040] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate for the embodiments of this application described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0041] As described in the background section, existing technologies have shortcomings in the precise control of drying and implantation depth of flexible microfilament electrodes, necessitating a more stable, efficient, and controllable drying and implantation method to overcome these technical deficiencies. To address these technical problems, embodiments of this application provide a pre-assembled structure, a pre-assembled method, and an implantation method for a microfilament electrode.

[0042] According to embodiments of this application, a pre-assembled structure for a microfilament electrode is provided, such as... Figure 1 and Figure 2 As shown, it includes: a microfilament electrode having a substrate 10 and multiple microfilament structures 20 attached to the substrate 10; an organic thin film 40 having microfilament structures 20 arranged on the organic thin film 40; and a rigid implantation tool fixedly connected to the microfilament structures 20, the rigid implantation tool being used to implant electrode recording sites on the microfilament structures 20 into a target object.

[0043] In the pre-assembled structure provided in this application embodiment, the microfilament electrode has a substrate and multiple microfilament structures attached to the substrate. The pre-assembled structure also includes an organic film and a rigid implantation tool. The microfilament structures are arranged on the organic film, and the rigid implantation tool is fixedly connected to the microfilament structures for implanting the electrode recording sites on the microfilament structures into the target object. Thus, during the drying process after the flexible microfilament electrode is separated from the silicon substrate, the aforementioned organic film prevents the microfilament structures from drifting, tangling, and breaking after drying, ensuring the spatial stability of the microfilament structures and facilitating subsequent implantation steps. This improves the implantation effect and performance of the pre-assembled microfilament electrode. Furthermore, the pre-assembled rigid implantation tool in the pre-assembled structure significantly shortens the surgical implantation time, improves implantation efficiency, and reduces surgical difficulty and risk. Simultaneously, the implantation depth of each microfilament structure can be independently controlled, facilitating precise implantation into the target brain region and improving the accuracy and reliability of the experiment.

[0044] Specifically, the substrate 10 of the microwire electrode is typically a planar or microstructured platform made of silicon, quartz, ceramic, or other rigid materials. The role of the substrate 10 is to provide stable support and carrier, enabling the microwire electrode to maintain its structural integrity and stability during fabrication, processing, and implantation.

[0045] Specifically, the aforementioned microfilament structure 20 is the core component of the microfilament electrode, made of conductive materials and flexible or stretchable insulating materials, typically possessing high tensile strength and biocompatibility. The diameter and length of the electrode microfilaments vary depending on the application requirements, and their surfaces may undergo special treatments, such as nanostructuring or coating, to enhance interfacial properties with the target object (e.g., neural tissue). The front end of the microfilament structure 20 serves as the implantation tip of the microfilament electrode, used for implantation into the target object. For example, the implantation tip can be inserted into neural tissue and directly contact bioelectrically active units such as neurons or cells. The implantation tip is typically very small, with a diameter possibly on the micrometer scale, to minimize damage to the target object and improve signal acquisition resolution. The implantation tip may contain one or more electrode sites, which can be exposed metal regions (e.g., gold, platinum, iridium), used to capture neural electrical activity signals or apply stimulation.

[0046] The microfilament electrode described in this application embodiment may further include a transmission section 30, such as... Figure 1 As shown, the transmission unit 30 connects the microfilament structure 20 to external circuitry and external devices, and is used to transmit the signals collected by the microfilament structure 20.

[0047] Specifically, in the drying process that separates the flexible microfilament electrode from the silicon substrate, the microfilament structure 20 of the microfilament electrode gradually detaches from the substrate 10 and forms a stable arrangement on the organic thin film 40 through the evaporation of the organic solvent. Drying the microfilament electrode on the organic thin film 40 reduces the influence of airflow on the microfilaments, avoiding irregular movement and spatial distribution uncertainties during the drying process. The organic thin film 40 provides support, preventing deformation and damage to the microfilament structure 20 during drying. This helps the microfilament electrode maintain a straight posture or a predetermined shape, ensuring the dried microfilament structure 20 retains a good morphology and improving electrode integrity before implantation, thus facilitating precise control of subsequent implantation depth. The application scenario of this embodiment can be in the field of neuroscience research, particularly in electrophysiological recording and stimulation experiments, where high-quality microfilament electrodes are required for precise implantation.

[0048] In some optional embodiments, the organic film 40 is made of at least one of parylene, polyimide, polydimethylsiloxane, polytetrafluoroethylene, polyethylene terephthalate, polyethylene, and polypropylene. These materials possess good chemical stability and mechanical properties, providing necessary support for the microfilament electrode during drying without affecting its biocompatibility. This improves the stability and safety of the microfilament electrode during drying and implantation, ensuring good contact between the electrode and the target object. The organic film 40 used in the embodiments of this application is not limited to the above-mentioned types, and this application does not impose specific limitations.

[0049] In this embodiment, microstructures can also be introduced onto the surface of the organic thin film 40. These microstructures can alter the actual contact area between the microfilament structure 20 and the film. For example, a grid or stripe structure effectively reduces the area of ​​completely flat contact between the two, decreasing the total area of ​​action of van der Waals forces and electrostatic forces, thereby weakening the adhesion. Conversely, a groove structure may embed microfilament electrodes, increasing the actual contact area and resulting in stronger adhesion. Thus, by optimizing the microstructure, an ideal adhesion effect can be achieved. It should be noted that the organic thin film 40 may also be without surface microstructures; this embodiment does not impose a specific limitation.

[0050] For example, the surface microstructure of the organic thin film 40 is selected from at least one of a mesh structure and a stripe structure. In the step of arranging multiple microfilament structures 20 on the organic thin film 40, the microfilament structures 20 are in contact with at least a portion of the surface microstructure. The aforementioned surface microstructure can increase the surface roughness of the organic thin film 40, thereby improving the adhesion properties between the microfilament structures 20 and the film.

[0051] Specifically, the mesh or stripe structure can provide more contact points, allowing the microfilament structure 20 to better adhere to the film during drying, preventing it from easily slipping or drifting. Simultaneously, the microfilament structure 20 can be more easily separated from the organic film 40 when needed without damaging it. Furthermore, the microstructure can serve as a template for the arrangement of the microfilament structure 20, guiding it to align according to a specific pattern. The mesh structure can promote the orderly arrangement of the microfilament structure 20 according to the grid's row and column layout, while the stripe structure may guide the microfilament structure 20 to be neatly distributed along the stripe direction. This orderly arrangement is beneficial for subsequent assembly and implantation.

[0052] In another example, the surface microstructure of the organic thin film 40 includes multiple groove structures. During the step of arranging multiple microfilament structures 20 on the organic thin film 40, the multiple microfilament structures 20 are embedded in the groove structures, and each microfilament structure 20 is embedded in a different groove structure. The groove structures can interact with the shape of the microfilament structures 20 to form a mechanical lock, thereby ensuring that the microfilament electrodes are not easily detached from the film during drying and handling. However, before implantation, the microfilament structures 20 can be easily separated from the film structure by specific external forces (such as heating, solvent action, or physical stretching).

[0053] Specifically, the groove structure in the aforementioned surface microstructure serves as a precise guide groove for the microfilament structure 20. Each microfilament structure 20 is embedded in a different groove, allowing them to be precisely arranged on the organic film 40 according to a predetermined spatial layout. This design helps ensure the relative positional relationship between the microfilament structures 20, facilitating subsequent assembly and implantation operations. Furthermore, the groove structure provides a clear interface when the microfilament structure 20 separates from the organic film 40, making the separation process easier to control and preventing damage to the microfilament electrodes.

[0054] In some alternative embodiments, the first surface of the organic thin film 40 is hydrophobic, and during the step of arranging the multiple microfilament structures 20 on the organic thin film 40, the microfilament structures 20 are in contact with at least a portion of the first surface. Utilizing the hydrophobic surface property of the organic thin film 40, moisture residue between the microfilament electrode and the organic thin film 40 can be reduced, thereby reducing the risk of adhesion of the microfilament structures 20 during the drying process, and thus improving the drying quality and efficiency of the microfilament electrode.

[0055] To make the surface of the organic thin film 40 hydrophobic, hydrophobic groups, such as alkyl chains, fluorides, or silane compounds, can be introduced onto the surface through chemical reactions, thereby improving the surface hydrophobicity. For example, surface modification with hexamethyldisilazane (HMDS) or perfluorooctyltriethoxysilane (FOTS) can establish a hydrophobic silanized layer that effectively repels water molecules. Alternatively, a hydrophobic compound, such as a fluorinated polymer or silica nanoparticles, can be deposited on the surface of the organic thin film 40 using physical vapor deposition (PVD) or chemical vapor deposition (CVD) techniques to achieve the same hydrophobic effect.

[0056] In some alternative implementations, such as Figure 1 As shown, each microfilament structure 20 includes a connected stretching portion 202 and an implantation end 201. The electrode recording site is located on the implantation end 201. During the process of implanting the electrode recording site of the microfilament structure 20 into the target object using a rigid implantation tool, the stretching portion 202 is in a stretched state under the action of the rigid implantation tool, thereby implanting the electrode recording site into the target object.

[0057] The rigid implantation tool described in this application refers to any elongated component capable of providing mechanical stability and support for assisting the implantation of flexible microfilament electrodes. Such tools should possess sufficient rigidity to maintain their shape during implantation, thereby guiding the microfilament electrode to the designated location. Simultaneously, the size and shape design of the assistive implantation tool should match the characteristics of the microfilament electrode to facilitate connection, pre-assembly, and separation. For example, the rigid implantation tool described above is an implantation needle. Such tools have a sharp tip for penetration into the target object, and their material can be made of metals (such as stainless steel, tungsten, gold, silver, etc.) or non-metallic materials (such as ceramics, glass fiber, carbon fiber, plastics, etc.), as long as these materials possess the required rigidity and are biocompatible.

[0058] In some alternative implementations, such as Figure 2 As shown, the rigid implantation tool described above is an implantation needle 70. Such tools have a sharp tip to facilitate penetration into the target object, and their materials can be made of metals (such as stainless steel, tungsten, gold, silver, etc.) or non-metallic materials (such as ceramics, glass fiber, carbon fiber, plastic, etc.), as long as these materials have the required rigidity and are biocompatible.

[0059] In some alternative implementations, such as Figure 2As shown, the pre-assembled structure in this embodiment further includes: an electrode housing 50, which has a plurality of receiving grooves 60, the same number as the microfilament structures 20, and the electrode housing 50 is fixedly connected to the substrate 10 so that the microfilament structures 20 correspond one-to-one with and are aligned with the receiving grooves 60, and the second surface of the substrate 10 of the microfilament electrode is parallel to the third surface of the electrode housing 50, wherein the second surface is the side surface of the substrate 10 on which the microfilament structures 20 are formed, and the third surface is the side surface of the electrode housing 50 on which the receiving grooves 60 are formed; the rigid implantation tool is a plurality of implantation needles 70, the same number as the microfilament structures, the end of the implantation needle 70 is fixedly connected to the end of each microfilament structure 20, and the implantation needles 70 are disposed one-to-one in the receiving grooves 60.

[0060] In the above optional embodiments, the electrode housing 50 may have the same number of parallel grooves (i.e., the aforementioned receiving grooves 60) as the microfilament structures 20. The spacing of these grooves is consistent with that of the electrode microfilaments, ensuring that the microfilament structures 20 can be precisely positioned and supported during assembly. The parallel grooves of the electrode housing 50 provide a position for each microfilament structure 20, preventing them from unnecessary movement or deformation during assembly and subsequent operations.

[0061] Specifically, the correspondence between the grooves on the electrode shell 50 and the electrode microfilaments allows for indirect control of the implantation depth of each microfilament structure 20's implantation end 201 by controlling the movement of the implantation needle 70 during implantation, facilitating precise control of the implantation depth. Before implantation, the pre-assembly of the grooves on the electrode shell 50 and the microfilament structure 20 within the electrode microfilaments makes the implantation needle 70 and the microfilament structure 20 a single unit, allowing for easier movement and fixation onto the positioning arm of the stereotaxic instrument. This method avoids the complexity of individually aligning each microfilament structure 20 and the implantation needle 70 during implantation, significantly improving implantation efficiency and accuracy. Furthermore, the electrode shell 50 also protects the electrode microfilaments during implantation, preventing damage during operation. Simultaneously, the shell isolates the microfilament structures 20, preventing mutual interference, especially during the implantation stage, helping to maintain the independence and functional integrity of each microfilament structure 20.

[0062] In the above optional embodiments, the implantation needle 70 serves as a rigid auxiliary tool, providing a stable guide for the implantation end 201 of the electrode microfilament. By bonding and fixing the implantation needle 70 to the electrode housing 50, it can be ensured that no relative displacement occurs between the implantation needle 70 and the electrode microfilament during the implantation process, thereby maintaining the accuracy of implantation guidance.

[0063] In the above optional embodiments, by making the second surface of the substrate 10 of the microfilament electrode parallel to the third surface of the electrode housing 50, it can be ensured that the grooves on the electrode housing 50 can be precisely aligned with the microfilament structure 20 in subsequent assembly steps, thereby achieving effective pre-assembly of the microfilament structure 20 with the implantation needle 70 (such as a tungsten needle).

[0064] Specifically, the surface of the electrode housing 50 is parallel to the substrate 10 (such as a silicon wafer), which means that the plane of the electrode housing 50 is consistent with the plane of the microfilament structure 20 of the electrode microfilament (i.e., the bottom surface of the trench). This ensures that when the electrode housing 50 is bonded and fixed to the substrate 10, the microfilament structure 20 can accurately fall into or fit into the trench of the housing without deviation.

[0065] In the above-described optional embodiments, the microfilament electrode and the rigid auxiliary implantation needle 70 can be considered to form a temporary "fixed connection" during the pre-assembly stage. However, this connection is intended for ease of operation during implantation and is not a permanent fixed connection. After implantation, the implantation needle 70 needs to be safely detached from the microfilament structure 20 so that the implantation end 201 of the microfilament structure 20 can remain in the tissue for long-term monitoring.

[0066] Specifically, the implantation end 201 of the microfilament structure 20 may have a circular hole. During the pre-assembly stage, the implantation end 201 can be connected to the implantation needle 70 through the circular hole. Alternatively, the implantation end 201 of the microfilament structure 20 can be connected to the implantation needle 70 using other methods, such as polyethylene glycol (PEG) adhesive. This connection ensures that the relative position between the electrode and the implantation needle 70 is fixed before implantation, facilitating control of the implantation needle 70 to guide the microfilament electrode to the target depth and position. When the electrode recording site on the implantation end 201 of the microfilament electrode is implanted into the target object, the implantation needle 70 plays a guiding and supporting role. The rigidity of the implantation needle 70 ensures that the electrode can stably penetrate the target object 80 and reach the specified depth.

[0067] For example, the implantation needle 70 is a tungsten needle. After the electrode shell 50 is fixed to the substrate 10 with epoxy glue, the groove on the electrode shell becomes a platform for the subsequent connection between the tungsten needle and the electrode microfilament. After the micropillar of the tungsten needle passes through the round hole at the tip of the electrode microfilament, the tungsten needle is placed and fixed in the groove of the electrode shell.

[0068] In some optional embodiments, the pre-assembled structure further includes a support tube, in which the implantation needle is partially disposed, with its end located outside the support tube. The support tube containing the implantation needle is disposed in a receiving groove. Introducing the support tube not only helps improve the pre-assembly process of the microfilament structure 20 and the implantation needle 70, but also helps optimize the implantation effect and the stability of the electrode after implantation. The main function of the support tube is to provide a certain vertical frictional force, so that the implantation needle 70 will not slip after pre-assembly, while also being able to move up and down under a certain force, i.e., implanting and withdrawing the implantation needle 70. After pre-assembly, the support tube fixes the implantation needle 70 and the implantation end of the microfilament structure 20. Therefore, the material of the support tube is not specifically limited in this embodiment, as long as it can provide a certain frictional force.

[0069] Specifically, during implantation into nerve tissue, the microfilament structure 20 of the microfilament electrode, due to its extremely small size and flexibility, requires rigid auxiliary tools such as the implantation needle 70 to guide it to the designated location. To prevent slight bending or displacement of the implantation needle 70 during implantation due to its elasticity or tissue resistance, which could affect the accuracy of implantation, the implantation needle 70 is passed through a support tube. The support tube provides additional rigid support along the length of the implantation needle 70, reducing elastic deformation of the implantation needle 70 and improving guidance and stability during implantation.

[0070] It should be noted that if the support tube is too long, it will affect the implantation depth and prevent the microfilament structure 20 from being implanted deeper. Therefore, the length of the support tube should be as short as possible while still being greater than the length of the receiving groove 60.

[0071] For example, the support tube is a polyimide tube (PI tube). The support tube can also be made of various insulating materials, such as polytetrafluoroethylene (PTFE), polycarbonate (PC), silicone rubber, etc., and the embodiments of this application do not specifically limit it.

[0072] It should be noted that the pre-assembled structure in this application embodiment does not require the introduction of an additional support tube. By setting multiple receiving grooves of the electrode shell as tubular through holes, the implantation needle is partially disposed in the through holes, and the end of the implantation needle is located outside the through holes. In this way, the through holes in the electrode shell provide a certain vertical friction force for the implantation needle. This can also improve the pre-assembly process of the microfilament structure and the implantation needle, and improve the implantation effect and the stability of the electrode after implantation.

[0073] According to embodiments of this application, a pre-assembly method for microfilament electrodes is also provided. Figure 3 This is a flowchart based on an embodiment of this application. For example... Figure 3 As shown, the drying method includes:

[0074] Step S1: Provide a microfilament electrode, which has a substrate and a microfilament structure attached to the substrate;

[0075] Step S2: Place the microfilament electrode in a container filled with organic solvent for drying. An organic film is placed inside the container so that the dried microfilament structure is arranged on the organic film.

[0076] Step S3: Pre-assemble the microfilament structure of the microfilament electrode with the rigid implantation tool to fix the microfilament structure to the rigid implantation tool, thereby obtaining the pre-assembled structure of the microfilament electrode described above.

[0077] In the pre-assembly method of the microfilament electrode provided in this application embodiment, the microfilament electrode is placed in a container filled with organic solvent for drying. An organic film is provided inside the container, and multiple dried microfilament structures are arranged on the organic film. This not only improves the drying efficiency of the microfilament electrode by reasonably selecting the type of organic solvent, but also prevents the microfilament structures from floating, tangling, and breaking after drying through the organic film, ensuring the spatial arrangement of the microfilament structures is stable. This provides convenience for subsequent implantation steps, thereby improving the implantation effect and performance of the pre-assembled microfilament electrode.

[0078] Exemplary embodiments of the pre-assembly method for the microfilament electrode according to this application will now be described in more detail with reference to the accompanying drawings. However, these exemplary embodiments may be implemented in many different forms and should be construed as limited to the embodiments set forth herein. It should be understood that these embodiments are provided so that the disclosure of this application is thorough and complete, and that the concept of these exemplary embodiments is fully conveyed to those skilled in the art.

[0079] First, proceed to step S1: as follows Figure 4 As shown, a microfilament electrode is provided, the microfilament electrode having a substrate 10 and a microfilament structure 20 attached to the substrate 10.

[0080] Specifically, the substrate 10 of the microwire electrode is typically a planar or microstructured platform made of silicon, quartz, ceramic, or other rigid materials. The role of the substrate 10 is to provide stable support and carrier, enabling the microwire electrode to maintain its structural integrity and stability during fabrication, processing, and implantation.

[0081] Specifically, the aforementioned microfilament structure 20 is the core component of the microfilament electrode, made of conductive materials and flexible or stretchable insulating materials, typically possessing high tensile strength and biocompatibility. The diameter and length of the electrode microfilaments vary depending on the application requirements, and their surfaces may undergo special treatments, such as nanostructuring or coating, to enhance interfacial properties with the target object (e.g., neural tissue). The front end of the microfilament structure 20 serves as the implantation tip of the microfilament electrode, used for implantation into the target object. For example, the implantation tip can be inserted into neural tissue and directly contact bioelectrically active units such as neurons or cells. The implantation tip is typically very small, with a diameter possibly on the micrometer scale, to minimize damage to the target object and improve signal acquisition resolution. The implantation tip may contain one or more electrode sites, which can be exposed metal regions (e.g., gold, platinum, iridium), used to capture neural electrical activity signals or apply stimulation.

[0082] The microfilament electrode described in this application embodiment may further include a transmission section 30, such as... Figure 4 As shown, the transmission unit 30 connects the microfilament structure 20 to external circuitry and external devices, and is used to transmit the signals collected by the microfilament structure 20.

[0083] After providing the aforementioned microfilament electrode, proceed to step S2: as follows Figure 1 As shown, the microfilament electrode is placed in a container filled with organic solvent for drying. An organic thin film 40 is placed inside the container, so that the dried microfilament structure 20 is arranged on the organic thin film 40.

[0084] Specifically, through the evaporation of the organic solvent, the microfilament structure 20 of the microfilament electrode gradually detaches from the substrate 10 and forms a stable arrangement on the organic film 40. Drying the microfilament electrode on the organic film 40 reduces the influence of airflow on the microfilaments, avoiding irregular movement and spatial distribution uncertainties during the drying process. The organic film 40 provides support, preventing deformation and damage to the microfilament structure 20 during drying. This helps the microfilament electrode maintain a straight posture or a predetermined shape, ensuring the dried microfilament structure 20 retains a good morphology and improving electrode integrity before implantation. This facilitates precise control of the subsequent implantation depth. The application scenario of this embodiment can be in the field of neuroscience research, particularly in electrophysiological recording and stimulation experiments, where high-quality microfilament electrodes are required for precise implantation.

[0085] For example, the aforementioned organic solvent includes anhydrous ethanol. The low boiling point (approximately 78°C) and high volatility of anhydrous ethanol allow it to evaporate rapidly, thereby accelerating the drying process and reducing the residence time of the microfilament electrode during processing. Furthermore, compared to water, anhydrous ethanol has a lower surface tension, which helps prevent the microfilament electrode from sticking or deforming during drying, maintaining its structural integrity. In addition, anhydrous ethanol has good compatibility with a variety of materials and will not corrode or damage the main components of the electrode microfilament (such as the metal conductor and polymer insulating layer).

[0086] It should be noted that the organic solvents used in the embodiments of this application are not limited to anhydrous ethanol. For example, the organic solvents mentioned above can also be selected from isopropanol, acetone, n-hexane and xylene, and the embodiments of this application do not make specific limitations.

[0087] In some optional embodiments, the organic film 40 is made of at least one of parylene, polyimide, polydimethylsiloxane, polytetrafluoroethylene, polyethylene terephthalate, polyethylene, and polypropylene. These materials possess good chemical stability and mechanical properties, providing necessary support for the microfilament electrode during drying without affecting its biocompatibility. This improves the stability and safety of the microfilament electrode during drying and implantation, ensuring good contact between the electrode and the target object. The organic film 40 used in the embodiments of this application is not limited to the above-mentioned types, and this application does not impose specific limitations.

[0088] In some alternative embodiments, during the process of placing the microfilament electrode in the container, the step of changing the microfilament structure 20 from an attached state to a separated state from the substrate 10, so that multiple microfilament structures 20 are arranged on the organic thin film 40, includes: as follows Figure 5 As shown, the substrate 10 is diced to remove a first target region from the substrate 10, wherein the first target region includes at least a portion of the substrate 10 in an attached state where the microfilament structure 20 is attached; as Figure 1 As shown, at least a portion of the organic film 40 is disposed between the microfilament structure 20 and the substrate 10, so that the dried microfilament structure 20 is arranged on the organic film 40, and the implantation end 201 of the microfilament structure 20 can partially extend beyond the organic film 40.

[0089] In the above optional embodiments, the contact state between the microfilament structure 20 and the substrate 10 is changed by physical means, so that it is naturally transferred to the organic film 40 during the drying process and forms an orderly arrangement, thereby improving the separation efficiency and stability of the microfilament structure 20, and also avoiding the adhesion of the microfilament electrode to other objects during the drying process, creating favorable conditions for subsequent assembly and implantation.

[0090] Specifically, the dicing process aims to remove the first target region in the substrate 10 through physical cutting, thereby releasing the microfilament implantation end attached to that region. This operation allows the portion of the microfilament structure 20 with the implantation end 201, which was originally tightly attached to the substrate 10, to detach and suspend, thus providing sufficient space for electrode implantation. After removing the first target region in the substrate 10, the microfilament structure 20 is supported and fixed by the organic film 40, enabling the microfilament structure 20 to be stably arranged on the organic film 40, preventing entanglement and breakage, and simplifying the preparation work before implantation; furthermore, during electrode implantation, the organic film 40 can be smoothly separated from the microfilament structure 20, solving the problem of difficulty in separating the microfilament structure from the substrate during implantation when directly drying without dicing.

[0091] In the above optional embodiments, the step of arranging multiple microfilament structures 20 on the organic film 40 may further include: after the step of disposing at least a portion of the organic film 40 between the microfilament structure 20 and the substrate 10, aspirating the organic solvent in a second target region, wherein the second target region is a local area in the container having the microfilament structure 20.

[0092] Specifically, as the microfilament structure 20 comes into contact with the organic film 40 and gradually attaches to the film during the drying process, absorbing the organic solvent helps maintain the morphology of the microfilament structure 20. The microfilament structure 20 may exhibit a certain degree of flexibility and fluidity in the solvent; once the solvent is absorbed, the microfilament structure 20 will be fixed in its current position, forming a stable layout. This fixed layout is crucial for subsequent assembly and implantation operations, ensuring the position and orientation of each microfilament structure 20. Furthermore, during the drying process of the microfilament structure 20, if the solvent evaporates naturally, solvent residue may cause adhesion between microfilaments or between microfilaments and the container wall, especially when the microfilament structures 20 are densely packed. Absorbing the solvent reduces this risk of adhesion.

[0093] After arranging the dried multifilament structure on the organic film, step S3 is performed: the microfilament structure of the microfilament electrode is pre-assembled with the rigid implantation tool to fix the microfilament structure to the rigid implantation tool, resulting in... Figure 2 The pre-assembled structure of the microwire electrode is shown.

[0094] In some alternative embodiments, the step of pre-assembling the microfilament structure 20 with the rigid implantation tool includes: as follows Figure 6As shown, an electrode housing 50 is provided, having a plurality of receiving grooves 60, the same number as the microfilament structures 20. The electrode housing 50 is fixedly connected to the substrate 10 so that the microfilament structures 20 correspond one-to-one with and are aligned with the receiving grooves 60, and the second surface of the substrate 10 of the microfilament electrode is parallel to the third surface of the electrode housing 50, wherein the second surface is the side surface of the substrate 10 on which the microfilament structures 20 are formed, and the third surface is the side surface of the electrode housing 50 on which the receiving grooves 60 are formed; Figure 2 As shown, the end of the implantation needle 70 is fixedly connected to the end of each microfilament structure 20, and the implantation needles 70 are arranged one by one in the receiving groove 60.

[0095] In the above-described optional embodiments, the electrode housing 50 has the same number of parallel grooves (i.e., the aforementioned receiving grooves 60) as the microfilament structures 20. The spacing of these grooves is consistent with that of the electrode microfilaments, ensuring that the microfilament structures 20 can be precisely positioned and supported during assembly. The parallel grooves of the electrode housing 50 provide a position for each microfilament structure 20, preventing them from unnecessary movement or deformation during assembly and subsequent operations.

[0096] In the above optional embodiments, the implantation needle 70 serves as a rigid auxiliary tool, providing a stable guide for the implantation end 201 of the electrode microfilament. By bonding and fixing the implantation needle 70 to the electrode housing 50, it can be ensured that no relative displacement occurs between the implantation needle 70 and the electrode microfilament during the implantation process, thereby maintaining the accuracy of implantation guidance.

[0097] Specifically, before implantation, the implantation needle 70 is fixed within the groove of the electrode housing 50 and secured, providing additional mechanical stability to the microfilament structure 20 and ensuring that the position and orientation of the microfilament structure 20 remain unchanged even if slight disturbances occur during surgical procedures. During the actual implantation procedure, the implantation needle 70 is not permanently fixed to the electrode housing 50. Although they are temporarily bonded together during the pre-assembly stage, the purpose of bonding the implantation needle 70 and the electrode housing 50 is to maintain the relative position of the microfilament structure 20 and the implantation needle 70 during operation, transfer, and preparation for implantation, ensuring accuracy and stability during implantation. However, during the implantation stage, the adhesive originally used to bond and fix the implantation needle 70 and the electrode housing 50 dissolves, thereby releasing the bond between the implantation needle 70 and the electrode housing 50, allowing the implantation needle 70 to separate from the implantation end 201 of the electrode microfilament after implantation to the predetermined depth.

[0098] In the above optional embodiments, by making the second surface of the substrate 10 of the microfilament electrode parallel to the third surface of the electrode housing 50, it can be ensured that the grooves on the electrode housing 50 can be precisely aligned with the microfilament structure 20 in subsequent assembly steps, thereby achieving effective pre-assembly of the microfilament structure 20 with the implantation needle 70 (such as a tungsten needle).

[0099] Specifically, the surface of the electrode housing 50 is parallel to the substrate 10 (such as a silicon wafer), which means that the plane of the electrode housing 50 is consistent with the plane of the microfilament structure 20 of the electrode microfilament (i.e., the bottom surface of the trench). This ensures that when the electrode housing 50 is bonded and fixed to the substrate 10, the microfilament structure 20 can accurately fall into or fit into the trench of the housing without deviation.

[0100] In the above-described optional embodiments, the microfilament electrode and the rigid auxiliary implantation needle 70 can be considered to form a temporary "fixed connection" during the pre-assembly stage. However, this connection is intended for ease of operation during implantation and is not a permanent fixed connection. After implantation, the implantation needle 70 needs to be safely detached from the microfilament structure 20 so that the implantation end 201 of the microfilament structure 20 can remain in the tissue for long-term monitoring.

[0101] Specifically, the implantation end 201 of the microfilament structure 20 may have a circular hole. During the pre-assembly stage, the implantation end 201 can be connected to the implantation needle 70 through the circular hole. Alternatively, the implantation end 201 of the microfilament structure 20 can be connected to the implantation needle 70 using other methods, such as polyethylene glycol (PEG) adhesive. These connections ensure that the relative position between the electrode and the implantation needle 70 is fixed before implantation, facilitating control of the implantation needle 70 to guide the microfilament electrode to the target depth and position.

[0102] For example, the implantation needle is a tungsten needle. After the substrate of the microfilament electrode is fixed to the electrode shell with epoxy glue, the groove on the electrode shell becomes a platform for the subsequent connection between the tungsten needle and the electrode microfilament. After the micropillar of the tungsten needle passes through the round hole at the tip of the electrode microfilament, the tungsten needle is placed and fixed in the groove of the electrode shell.

[0103] In the above optional embodiments, the step of pre-assembling the microfilament structure 20 of the microfilament electrode with the rigid implantation tool may further include: before the step of fixing the end of the implantation needle 70 to the end of the microfilament structure 20, partially placing the implantation needle 70 in the support tube, with the end of the implantation needle 70 located outside the support tube; in the step of placing the implantation needle 70 in the receiving groove 60, placing the support tube containing the implantation needle 70 in the receiving groove 60. Introducing the support tube not only helps improve the pre-assembly process of the microfilament structure 20 and the implantation needle 70, but also helps optimize the implantation effect and the stability of the electrode after implantation. The main function of the support tube is to provide a certain vertical frictional force, so that the implantation needle 70 will not slip after pre-assembly, while also being able to move up and down under a certain force, i.e., implanting and withdrawing the implantation needle 70. After pre-assembly, the support tube fixes the implantation needle 70 and the implantation end of the microfilament structure 20. Therefore, the material of the support tube is not specifically limited in this application embodiment, as long as it can provide a certain frictional force.

[0104] Specifically, during implantation into nerve tissue, the microfilament structure 20 of the microfilament electrode, due to its extremely small size and flexibility, requires rigid auxiliary tools such as the implantation needle 70 to guide it to the designated location. To prevent slight bending or displacement of the implantation needle 70 during implantation due to its elasticity or tissue resistance, which could affect the accuracy of implantation, the implantation needle 70 is passed through a support tube. The support tube provides additional rigid support along the length of the implantation needle 70, reducing elastic deformation of the implantation needle 70 and improving guidance and stability during implantation.

[0105] It should be noted that if the support tube is too long, it will affect the implantation depth and prevent the microfilament structure 20 from being implanted deeper. Therefore, the length of the support tube should be as short as possible while still being greater than the length of the receiving groove 60.

[0106] For example, the support tube is a polyimide tube (PI tube). The support tube can also be made of various insulating materials, such as polytetrafluoroethylene (PTFE), polycarbonate (PC), silicone rubber, etc., and the embodiments of this application do not specifically limit it.

[0107] It is worth noting that in the pre-assembly step of the microfilament structure with the rigid implantation tool, it is not necessary to introduce an additional support tube. By setting multiple receiving slots of the electrode shell as tubular through holes, the implantation needle is partially placed in the through holes, with the end of the implantation needle located outside the through holes. In this way, the through holes in the electrode shell can be used to provide a certain vertical friction force for the implantation needle. This can also improve the pre-assembly process of the microfilament structure and the implantation needle, and improve the implantation effect and the stability of the electrode after implantation.

[0108] According to embodiments of this application, a method for implanting a microwire electrode is also provided. Figure 7This is a flowchart based on an embodiment of this application. For example... Figure 7 As shown, the implantation method includes the following steps:

[0109] Step S10: Provide a pre-assembled structure for the microfilament electrode in the above embodiments;

[0110] Step S20: The electrode recording sites of the microfilament structure are implanted into the target object using a rigid implantation tool. During the implantation of the electrode recording sites, the microfilament structure and the organic film change from an attached state to a separated state.

[0111] The implantation method provided in this application significantly shortens the surgical implantation time, improves implantation efficiency, and reduces surgical difficulty and risk by using a pre-assembled rigid implantation tool. Simultaneously, the implantation depth of each microfilament structure can be independently controlled, facilitating precise implantation into the target brain region and improving the accuracy and reliability of the experiment.

[0112] Exemplary embodiments of the microfilament electrode implantation method according to this application will now be described in more detail with reference to the accompanying drawings. However, these exemplary embodiments may be implemented in many different forms and should be construed as being limited to the embodiments set forth herein. It should be understood that these embodiments are provided so that the disclosure of this application is thorough and complete, and that the concept of these exemplary embodiments is fully conveyed to those skilled in the art.

[0113] First, proceed to step S10: (e.g.) Figure 1 and Figure 2 As shown, a pre-assembled structure for a microfilament electrode is provided.

[0114] Specifically, the pre-assembled structure of the microfilament electrode provided in this application embodiment can ensure the stable spatial arrangement of the microfilament structure 20. Then, after the microfilament structure 20 is pre-assembled with the rigid implantation tool, the guiding effect of the rigid implantation tool can achieve precise implantation of the implantation end in the microfilament electrode, which improves the accuracy and efficiency of implantation and reduces damage and risks during the implantation process.

[0115] After providing the pre-assembled structure of the microfilament electrode, step S20 is performed: as follows Figures 8 to 10 As shown, the electrode recording sites of the microfilament structure 20 are implanted into the target object 80 using a rigid implantation tool. During the implantation of the electrode recording sites, the microfilament structure 20 and the organic film 40 change from an attached state to a separated state.

[0116] Specifically, such as Figure 8 and Figure 9As shown, each microfilament structure 20 includes a connected stretching portion 202 and an implantation end 201. The electrode recording site is located on the implantation end 201. The electrode recording site of the microfilament structure 20 is implanted into the target object 80 using a rigid implantation tool, including: making the stretching portion 202 in a stretched state under the action of the rigid implantation tool to implant the electrode recording site into the target object 80.

[0117] The rigid implantation tool described in this application refers to any elongated component capable of providing mechanical stability and support for assisting the implantation of flexible microfilament electrodes. Such tools should possess sufficient rigidity to maintain their shape during implantation, thereby guiding the microfilament structure 20 to the designated location. Simultaneously, the size and shape design of the assistive implantation tool should match the characteristics of the microfilament structure 20 to facilitate connection, pre-assembly, and separation. For example, the rigid implantation tool described above is an implantation needle. Such tools have a sharp tip for penetration into the target object, and their material can be made of metals (such as stainless steel, tungsten, gold, silver, etc.) or non-metallic materials (such as ceramics, glass fiber, carbon fiber, plastic, etc.), provided that these materials possess the required rigidity and are biocompatible.

[0118] The following describes the implantation method of the microfilament electrode in the embodiments of this application using a rigid implantation tool as the implantation target. That is, the rigid implantation tool is multiple implantation needles with the same number as the microfilament structure. However, it should be noted that the rigid implantation tool used in the embodiments of this application is not limited to the above-mentioned implantation needles, and can also be other rigid tools.

[0119] In some optional embodiments, the pre-assembled structure of the microfilament electrode is obtained using the pre-assembly method of the microfilament electrode described in the above embodiments. Specifically, the step of pre-assembling the microfilament structure 20 with the rigid implantation tool includes: as follows Figure 6 As shown, an electrode housing 50 is provided, having a plurality of receiving grooves 60, the same number as the microfilament structures 20. The electrode housing 50 is fixedly connected to the substrate 10 so that the microfilament structures 20 correspond one-to-one with and are aligned with the receiving grooves 60, and the second surface of the substrate 10 of the microfilament electrode is parallel to the third surface of the electrode housing 50, wherein the second surface is the side surface of the substrate 10 on which the microfilament structures 20 are formed, and the third surface is the side surface of the electrode housing 50 on which the receiving grooves 60 are formed; Figure 2 As shown, the end of the implantation needle 70 is fixedly connected to the end of each microfilament structure 20, and the implantation needles 70 are correspondingly placed in the receiving grooves 60; as Figure 9 As shown, in the step of implanting the electrode recording site on the implantation end 201 into the target object 80, a positioning device is used to control the movement of the implantation needle 70 so as to implant the electrode recording site on the implantation end 201 into the target depth in the target object 80.

[0120] In the above optional embodiments, the electrode housing 50 has the same number of parallel grooves (i.e., the aforementioned receiving grooves 60) as the microfilament structures 20. The spacing of these grooves is consistent with that of the electrode microfilaments, ensuring that the microfilament structures 20 can be precisely positioned and supported during assembly. The parallel grooves of the electrode housing 50 provide a position for each microfilament structure 20, preventing them from unnecessary movement or deformation during assembly and subsequent operations.

[0121] Specifically, the correspondence between the grooves on the electrode shell 50 and the electrode microfilaments allows for indirect control of the implantation depth of each microfilament structure 20's implantation end 201 by controlling the movement of the implantation needle 70 during implantation, facilitating precise control of the implantation depth. Before implantation, the pre-assembly of the grooves on the electrode shell 50 and the microfilament structure 20 within the electrode microfilaments makes the implantation needle 70 and the microfilament structure 20 a single unit, allowing for easier movement and fixation onto the positioning arm of the stereotaxic instrument. This method avoids the complexity of individually aligning each microfilament structure 20 and the implantation needle 70 during implantation, significantly improving implantation efficiency and accuracy. Furthermore, the electrode shell 50 also protects the electrode microfilaments during implantation, preventing damage during operation. Simultaneously, the shell isolates the microfilament structures 20, preventing mutual interference, especially during the implantation stage, helping to maintain the independence and functional integrity of each microfilament structure 20.

[0122] In the above optional embodiments, the implantation needle 70 serves as a rigid auxiliary tool, providing a stable guide for the implantation end 201 of the electrode microfilament. By bonding and fixing the implantation needle 70 to the electrode housing 50, it can be ensured that no relative displacement occurs between the implantation needle 70 and the electrode microfilament during the implantation process, thereby maintaining the accuracy of implantation guidance.

[0123] Specifically, before implantation, the implantation needle 70 is fixed within the groove of the electrode housing 50 and secured, providing additional mechanical stability to the microfilament structure 20 and ensuring that the position and orientation of the microfilament structure 20 remain unchanged even if slight disturbances occur during surgical procedures. During the actual implantation procedure, the implantation needle 70 is not permanently fixed to the electrode housing 50. Although they are temporarily bonded together during the pre-assembly stage, the purpose of bonding the implantation needle 70 and the electrode housing 50 is to maintain the relative position of the microfilament structure 20 and the implantation needle 70 during operation, transfer, and preparation for implantation, ensuring accuracy and stability during implantation. However, during the implantation stage, the adhesive originally used to bond and fix the implantation needle 70 and the electrode housing 50 dissolves, thereby releasing the bond between the implantation needle 70 and the electrode housing 50, allowing the implantation needle 70 to separate from the implantation end 201 of the electrode microfilament after implantation to the predetermined depth.

[0124] like Figure 9As shown, when the electrode recording site on the implantation end 201 of the microfilament electrode is implanted into the target object 80, the implantation needle 70 plays a guiding and supporting role. The rigidity of the implantation needle 70 ensures that the electrode can stably penetrate into the target object 80 and reach the specified depth. For example, in the step of implanting the electrode recording site into the target object 80, a positioning device is used to control the movement of the implantation needle 70 to implant the electrode recording site on the implantation end 201 into the target depth in the target object 80.

[0125] like Figure 10 As shown, once the implantation end of the microfilament electrode reaches the target depth, the implantation needle will withdraw from the circular hole at the electrode tip or separate from the microfilament electrode by dissolving the fixing material, allowing the implantation end to remain independently in the target object 80. This separation process typically does not damage the electrode or the implantation needle, ensuring both reusability and the electrode's independent operational capability after implantation. It should be noted that the method of dissolving the fixing material described above is applicable to situations where the implantation end 201 of the PEG-adhesive microfilament structure 20 is connected to the implantation needle 70. The PEG adhesive will automatically dissolve and separate after implantation, thus requiring no additional processing.

[0126] In some alternative implementations, such as Figures 9 to 11 As shown, the steps of implanting the electrode recording site on the implantation end 201 into the target object 80 using a rigid implantation tool include: simultaneously fixing the tails of multiple implantation needles 70 using a fixing module 90, wherein the fixing module 90 has multiple fixing parts corresponding one-to-one with the implantation needles 70, and the fixing parts are used to make the portions of any two implantation needles 70 outside the fixing module 90 have the same or different lengths; and controlling the movement of the implantation needles 70 using the fixing module 90 to implant the electrode recording site on the implantation end 201 into the target depth in the target object 80.

[0127] In the above optional embodiments, the simultaneous and precise implantation of multiple implantation needles 70 is achieved through the control function of the fixing module 90, thereby improving the efficiency and accuracy of implantation. In particular, for microwire electrodes that need to be implanted at different depths, one-time precise implantation can be achieved, reducing surgical time and complexity.

[0128] Specifically, the aforementioned fixation module 90 is a specially designed component intended to simultaneously fix the tails of all implantation needles 70 during implantation, ensuring their precise positioning and control as a whole. The fixation module 90 has multiple slots or holes that match the shape and size of the tails of the implantation needles 70, allowing for the stable insertion of each needle's tail. Furthermore, since the implantation needles 70 are not directly fixed to the electrode housing 50, the process of separating them from the tissue after implantation is simpler. After the implantation operation is completed, the fixation module 90 can be removed as a whole, along with the implantation needles 70, while the implanted end 201 of the electrode microfilament remains in the tissue. This reduces the risk of damage to the electrode microfilament and facilitates the recovery and reuse of the implantation needles 70.

[0129] Furthermore, each slot or hole of the fixing module 90 can correspond to an implantation needle 70. Their design can take into account the difference in implantation depth of the electrode microfilaments. That is, each slot or hole of the fixing module 90 can be designed to a different depth. This means that even if all implantation needles 70 move at the same time, the implantation depth of each microfilament structure 20 can still be adjusted as needed.

[0130] Specifically, the fixing module 90 has multiple slots of varying depths. These slots are used to simultaneously fix the tails of multiple implantation needles. The depth of the slots corresponds to the final implantation depth of the microfilament electrode, with deeper slots actually assigned to implantation needles requiring shallower implantation depths. When the fixing module 90 descends as a whole, all implantation needles move downwards synchronously. However, due to the differences in slot depths within the fixing module 90, different implantation needles can move different distances. For the implantation needles with deeper slots, because their "extra" length on the fixing module 90 is occupied by the slots, the actual implantation depth of these needles increases less when the fixing module 90 descends the same distance. Therefore, after the fixing module 90 descends a certain distance, implantation needles with shallower slots, due to their smaller "extra" length, will be implanted deeper; while implantation needles with deeper slots, due to their larger "extra" length, will be implanted at a relatively shallower depth. In this way, by adjusting the depth of each slot on the fixing module 90, precise control of the implantation depth of different microfilament electrodes can be achieved while maintaining the same needle length.

[0131] In the embodiments of this application, the methods for removing the organic film by external force include, but are not limited to: directly applying physical tension or pressure to peel the microfilament structure from the film; if the adhesion between the organic film and the microfilament structure is temperature-dependent, the adhesion can be weakened by heating to separate the two; using a solvent that can dissolve or soften the film material can destroy the adhesion between the film and the electrode, making separation easier; for organic films made of special materials, the adhesion between the two can be weakened by triggering electrochemical or chemical reactions.

[0132] It should also be noted that the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element.

[0133] The above are merely embodiments of this application and are not intended to limit the scope of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of the claims of this application.

Claims

1. A method for pre-assembling a microwire electrode, characterized in that, Includes the following steps: A microfilament electrode is provided, the microfilament electrode having a substrate and a multifilament structure attached to the substrate; The microfilament electrode is placed in a container filled with organic solvent for drying. An organic film is disposed inside the container. During the process of placing the microfilament electrode in the container, the microfilament structure changes from an attached state to a separated state with the substrate. The substrate is then diced to remove a first target region from the substrate. The first target region includes at least a portion of the substrate in the attached state where the microfilament structure is attached. At least a portion of the organic film is disposed between the microfilament structure and the substrate, such that the dried microfilament structure is arranged on the organic film; The organic solvent in the second target region is absorbed, wherein the second target region is a local region in the container having the microfilament structure; The microfilament structure of the microfilament electrode is pre-assembled with a rigid implantation tool to fix the microfilament structure to the rigid implantation tool, thereby obtaining the pre-assembled structure of the microfilament electrode.

2. The pre-assembly method according to claim 1, characterized in that, The rigid implantation tool consists of multiple implantation needles, the same number as the microfilament structure. The step of pre-assembling the microfilament structure with the rigid implantation tool includes: An electrode housing is provided, the electrode housing having a plurality of receiving slots in the same number as the microfilament structure; The electrode shell is fixedly connected to the substrate so that the microfilament structure corresponds to and is aligned with the receiving groove one by one, and the second surface of the substrate of the microfilament electrode is parallel to the third surface of the electrode shell, wherein the second surface is the side surface of the substrate on which the microfilament structure is formed, and the third surface is the side surface of the electrode shell on which the receiving groove is located. The end of the implantation needle is fixedly connected to the end of each of the microfilament structures, and the implantation needles are placed one by one in the receiving groove.

3. The pre-assembly method according to claim 2, characterized in that, The step of pre-assembling the microfilament structure with the rigid implant tool further includes: Before the step of fixing the end of the implantation needle to the end of the microfilament structure, the implantation needle is partially disposed in the support tube, with the end of the implantation needle located outside the support tube; In the step of placing the implantation needle in the receiving groove, the support tube with the implantation needle is placed in the receiving groove.

4. A pre-assembled structure for a microwire electrode, characterized in that, The pre-assembled structure is obtained by the pre-assembly method according to any one of claims 1 to 3, and the pre-assembled structure includes: A microfilament electrode having a substrate and multiple microfilaments attached to the substrate; An organic thin film, wherein the microfilament structure is arranged on the organic thin film; A rigid implantation tool is fixedly connected to the microfilament structure, and the rigid implantation tool is used to implant electrode recording sites on the microfilament structure into a target object.

5. The pre-assembled structure of the microfilament electrode according to claim 4, characterized in that, The organic film is made of at least one of the following materials: parylene, polyimide, polydimethylsiloxane, polytetrafluoroethylene, polyethylene terephthalate, polyethylene, and polypropylene.

6. The pre-assembled structure of the microfilament electrode according to claim 4, characterized in that, One side of the organic thin film has a surface microstructure: The surface microstructure is selected from at least one of mesh structure and stripe structure, and the microfilament structure is in contact with at least a portion of the surface microstructure; or The surface microstructure includes multiple groove structures, and multiple microfilament structures are embedded in the groove structures, with each microfilament structure embedded in a different groove structure.

7. The pre-assembled structure of the microfilament electrode according to claim 4, characterized in that, The first surface of the organic film is hydrophobic, and the microfilament structure is in contact with at least a portion of the first surface.

8. The pre-assembled structure of the microfilament electrode according to any one of claims 4 to 7, characterized in that, The pre-assembled structure also includes: An electrode housing has a plurality of receiving slots, the same number as the microfilament structure, and the electrode housing is fixedly connected to the substrate so that the microfilament structure corresponds to and is aligned with the receiving slots one by one. The second surface of the substrate of the microfilament electrode is parallel to the third surface of the electrode housing, wherein the second surface is the side surface of the substrate on which the microfilament structure is formed, and the third surface is the side surface of the electrode housing on which the receiving slots are located. The rigid implantation tool consists of multiple implantation needles, the same number as the microfilament structure. The ends of the implantation needles are fixedly connected to the ends of each microfilament structure, and the implantation needles are arranged one-to-one in the receiving groove.

9. The pre-assembled structure of the microfilament electrode according to claim 8, characterized in that, It also includes a support tube, in which the implantation needle is partially disposed, and the end of the implantation needle is located outside the support tube. The support tube containing the implantation needle is disposed in the receiving groove.

10. The pre-assembled structure of the microfilament electrode according to claim 8, characterized in that, The electrode housing has multiple receiving slots that are tubular through holes. The implantation needle is partially disposed in the through holes, and the end of the implantation needle is located outside the through holes.

11. The pre-assembled structure of the microfilament electrode according to claim 4, characterized in that, Each of the microfilament structures includes a connected stretching portion and an implantation end, with the electrode recording site located on the implantation end. The stretching portion is used to be stretched under the action of the rigid implantation tool to implant the electrode recording site into the target object.

12. The pre-assembled structure of the microfilament electrode according to claim 4, characterized in that, The rigid implantation tool consists of multiple implantation needles, the same number as the microfilament structure, and the pre-assembled structure further includes: A fixing module is used to simultaneously fix the tails of multiple implanted needles. The fixing module has multiple fixing parts corresponding one-to-one with each implanted needle. The fixing parts are used to make the portions of any two implanted needles outside the fixing module have the same or different lengths.

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