Stichopus japonicus fry cultivation method

Abstract

The invention discloses a stichopus japonicus fry cultivation method, and belongs to the technical field of stichopus japonicus fry cultivation. Comprising the following steps: splashing a fermented feed containing a bacillus subtilis fermentation liquid and a bacterial liquid containing an enterococcus faecalis fermentation liquid and a lactobacillus casei fermentation liquid into a culture pond of which the pond bottom is paved with a polyethylene corrugated plate substratum; the fermented feed and the bacterial liquid are continuously splashed until the water quality in the culture pond is clear and transparent, the feed on the corrugated plate is fluffy and is in a short villus shape, and a stable beneficial bacterium phase is formed; peeling off the juvenile sea cucumbers on the 40th day after metamorphosis and attachment, distributing the juvenile sea cucumbers into a cultivation pond for cultivation, and feeding fermented feed and bacterial liquid every day. The method is applied to the aspect of stichopus japonicus fry cultivation, the problems that in existing stichopus japonicus fry production, diseases occur frequently, most fry breeding fields face poor fry quality, and the yield is low are solved, a stable cultivation environment dominated by beneficial bacteria is built, external multi-dimensional disease sources are buffered, blocked and eliminated continuously, and the survival rate of the stichopus japonicus fry is increased. The stichopus japonicus seedlings can grow in the environment without stress.

Description

Technical Field

[0001] The invention belongs to the technical field of sea cucumber seedling cultivation, and in particular relates to a sea cucumber seedling cultivation method. Background Art

[0002] The sea cucumber, scientifically known as Apostichopus japonicus, is a species of echinoderm belonging to the family Apostichopidae in the order Apostachidae. It is large, cylindrical or square-prism-shaped, with a thick, soft body wall and well-developed papillae on its dorsal surface. Rich in 18 amino acids, sea cucumber saponins, polysaccharides, taurine, various minerals, and active ingredients, it is economically valuable and a key aquaculture species in my country. Its optimal temperature range is 8°C-18°C and its optimal salinity range is 25‰-35‰. Its primary diet consists of organic matter such as seafloor detritus, benthic diatoms, and bacteria. After two to three years of growth, sea cucumbers reach an average weight of approximately 300 grams. Sea cucumber aquaculture began in northern my country in the 1990s. Due to the high prevalence of shrimp diseases in ponds, sea cucumbers quickly replaced shrimp as the primary aquaculture target in northern marine ponds. Today, sea cucumbers have become a pillar of pond and marine aquaculture in northern my country. Two major aquaculture areas have emerged in Liaoning and Shandong provinces, with expansion into Hebei and Fujian provinces.

[0003] In 1973, my country's Yellow Sea Fisheries Research Institute undertook a research project and national public relations project on artificial sea cucumber seedling cultivation technology. Breakthroughs in seedling cultivation and northern sea cucumber farming techniques quickly made this species a valuable single-species industry. Beginning in the 1990s, Chinese scientists began developing selective breeding programs based on populations imported from Japan and Russia. These programs have resulted in the development of new sea cucumber varieties, including "Shuiyuan No. 1," "Anyuan No. 1," and "Dongke No. 1," making significant contributions to the development of my country's sea cucumber aquaculture industry.

[0004] However, in recent years, with the continuous changes in the environment of aquaculture sea areas and irregular operations in the industry, diseases have occurred frequently in the production of sea cucumber seedlings. Most hatcheries are faced with problems such as poor seedling quality and low yield, which have seriously affected the healthy and sustainable development of the industry. Summary of the Invention

[0005] In view of the shortcomings of the existing technology, the technical problem to be solved by the present invention is to overcome the problems of frequent occurrence of diseases in the existing sea cucumber seedling production, poor seedling quality and low yield faced by most nurseries, which seriously affect the healthy and sustainable development of the industry. A sea cucumber seedling cultivation method is proposed, which is conducive to constructing a stable breeding environment dominated by beneficial bacteria, has a sustainable buffer barrier and eliminates external multi-dimensional pathogens, and allows sea cucumber seedlings to grow without stress in this environment.

[0006] In order to solve the technical problem, the technical solution adopted by the present invention is:

[0007] The present invention provides a method for cultivating sea cucumber seedlings, comprising:

[0008] Sprinkling fermented feed containing Bacillus subtilis fermentation liquid and bacterial liquid containing Enterococcus faecalis fermentation liquid and Lactobacillus casei fermentation liquid into a cultivation pool whose bottom is covered with a polyethylene corrugated board attachment base;

[0009] Continuously sprinkle fermented feed and bacterial liquid until the water in the cultivation pool is clear and bright, the feed on the corrugated plate is fluffy and short and thick, and a stable beneficial bacteria phase is formed. Change the water to reduce nitrite to less than 0.02mg / L;

[0010] The young ginseng on the 40th day of metamorphosis was peeled off and placed in the cultivation pond for cultivation, and was fed with fermented feed and bacterial liquid every day.

[0011] Preferably, the strain of Bacillus subtilis is bio-67384, with a preservation number of CMCC(B)63501; the strain of Enterococcus faecalis is bio-53874, with a preservation number of CMCC(B)32482; and the strain of Lactobacillus casei is bio-67414, with a preservation number of CGMCC NO.15409.

[0012] Preferably, the dosage of Bacillus subtilis fermentation broth is 2-2.5 ml / m 3 The dosage of Enterococcus faecalis fermentation liquid is 30-50ml / m 3 .

[0013] Preferably, the carbon-nitrogen ratio of the fermented feed is 10:1;

[0014] The fermented feed is prepared by the following method: adding 1% of brewer's yeast and 10% of lactobacillus casei fermentation liquid to the feed weight, performing semi-solid anaerobic fermentation, and then uniformly mixing with 3 times the weight of dry sea mud to obtain the fermented feed.

[0015] Preferably, the 40th day after the metamorphosis, the young ginseng is peeled off and planted in the cultivation pond for cultivation. The seedling specification is 0.025g / head and the seedling density is 150-200g / m 3 .

[0016] Preferably, feeding the fermented feed and bacterial liquid to the target weight every day includes:

[0017] Change the water to control the nitrite content in the culture tank within 0.5mg / L, feed the fermented feed and bacterial solution once a day, and the initial feeding amount is 50-60g / m 3 , increase by 10g / m every 7 days 3 , increased to 220g / m 3 No more increase after that.

[0018] Preferably, Bacillus subtilis is aerobic fermented for 72 hours in clean seawater disinfected at a salinity of 25 to obtain a Bacillus subtilis fermentation liquid; and Enterococcus faecalis and Lactobacillus casei are anaerobic fermented for 72 hours in clean seawater disinfected at a salinity of 25 to obtain Enterococcus faecalis fermentation liquid and Lactobacillus casei fermentation liquid, respectively.

[0019] Preferably, the fermented feed and bacterial liquid are continuously sprayed for 15 days until the water in the cultivation pool is clear and transparent, the feed on the corrugated plate is fluffy and short and thick, and a stable beneficial bacteria phase is formed, and the water is changed to reduce nitrite to within 0.02 mg / L.

[0020] Preferably, the bottom of the sea cucumber cement culture pond is covered with a polyethylene corrugated board attachment base used for sea cucumber seedlings, and the corrugated board is placed parallel to the pond bottom. After placement, the cement pond is filled with clean seawater and aerated, and the water temperature is controlled at 22°C.

[0021] Preferably, the method of peeling off the young ginseng that has been attached abnormally for 40 days and distributing them into the cultivation pond comprises: stopping the air flow to evenly distribute the young ginseng that has been attached abnormally for 40 days into the cultivation pond, and resuming the inflation after 10 minutes.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The present invention provides a sea cucumber seedling cultivation method. After first constructing a stable beneficial bacterial phase in a cultivation pond, young sea cucumbers that have undergone metamorphosis and attachment for 40 days are peeled off and placed in the cultivation pond. This method can effectively prevent the sea cucumber seedlings from being infected by harmful bacteria, reduce the use of antibiotics and disinfectants, and achieve zero antibiotics and zero disinfectants in the entire process. The final weight of the sea cucumber seedlings is about 25 times that of the initial weight, thereby ensuring the smooth implementation of the seedling production plan and promoting the healthy and sustainable development of the sea cucumber breeding industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The present invention provides a flow chart of a method for cultivating sea cucumber seedlings. DETAILED DESCRIPTION

[0025] The following is a detailed and complete description of the technical solutions in specific embodiments of the present invention, with reference to the accompanying drawings. It should be understood that the described embodiments are merely partial implementations of the overall technical solution of the present invention, and are not exhaustive. All other embodiments derived by those skilled in the art based on the overall concept of the present invention are intended to fall within the scope of protection of the present invention.

[0026] The present invention provides a method for cultivating sea cucumber seedlings, comprising: pouring a fermented feed containing Bacillus subtilis fermentation liquid and a bacterial liquid containing Enterococcus faecalis fermentation liquid and Lactobacillus casei fermentation liquid into a cultivation pool whose bottom is covered with a polyethylene corrugated board attachment base. This technical solution uses Bacillus subtilis fermentation liquid, Enterococcus faecalis fermentation liquid, and Lactobacillus casei fermentation liquid to construct a stable beneficial bacterial phase. The ecological occupation of the three beneficial bacteria can inhibit the reproduction of harmful bacteria. In this process, the fermented feed and the bacterial liquid are poured into the cultivation pool whose bottom is covered with a polyethylene corrugated board attachment base. The corrugated board serves as a carrier for the fermented feed, which can prevent the feed from directly falling on the bottom of the pool and deteriorating the environment. At the same time, the corrugated board also serves as an attachment base for the sea cucumber seedlings.

[0027] The present invention provides a method for cultivating sea cucumber seedlings, comprising: continuously spraying fermented feed and bacterial liquid into a cultivation pool so that the water quality is clear and transparent, the feed on the corrugated plate is fluffy and short and thick and hairy, a stable beneficial bacteria phase is formed, and changing the water to reduce nitrite to within 0.02 mg / L. The technical solution stipulates that the water quality is clear and transparent when the fermented feed and bacterial liquid are continuously sprayed into a cultivation pool so that the water quality is clear and transparent, the feed on the corrugated plate is fluffy and short and thick and hairy, and a stable beneficial bacteria phase is formed. At this time, the growth and reproduction of harmful bacteria are inhibited, and the sea cucumber seedlings grow rapidly. In addition, the technical solution also stipulates that changing the water to reduce nitrite to within 0.02 mg / L is because a higher concentration of nitrite will inhibit the growth of sea cucumber seedlings. Further, the absence of Vibrio when monitoring with TCBS culture medium indicates that a stable beneficial bacteria phase has been formed.

[0028] The present invention provides a method for cultivating sea cucumber seedlings, comprising: removing young sea cucumbers on the 40th day of metamorphosis and placing them in a cultivation pond for cultivation, feeding them with fermented feed and bacterial liquid daily. The technical solution specifically limits the transfer of the young sea cucumbers to a cultivation pond containing a stable beneficial bacterial phase to the 40th day of metamorphosis, as the original cultivation pond environment deteriorates beyond 40 days, making it less conducive to the growth of the sea cucumber seedlings.

[0029] In a preferred embodiment, the strain of Bacillus subtilis is bio-67384, with a deposit number of CMCC(B)63501; the strain of Enterococcus faecalis is bio-53874, with a deposit number of CMCC(B)32482; and the strain of Lactobacillus casei is bio-67414, with a deposit number of CGMCC NO.15409.

[0030] In a preferred embodiment, the amount of Bacillus subtilis fermentation broth is 2-2.5 ml / m 3 The dosage of Enterococcus faecalis fermentation liquid is 30-50ml / m 3 The technical solution limits the dosage of Bacillus subtilis fermentation liquid and Enterococcus faecalis fermentation liquid because the dosage can quickly form a beneficial bacterial phase. The dosage of Bacillus subtilis fermentation liquid and Enterococcus faecalis fermentation liquid is based on the volume of Bacillus subtilis fermentation liquid per cubic meter of the cultivation tank. It is understandable that the dosage of Bacillus subtilis fermentation liquid can also be 2.1 ml / m 3 , 2.2ml / m 3 , 2.3ml / m 3 , 2.4ml / m 3 At any point within the range, the dosage of Enterococcus faecalis fermentation broth can also be 35ml / m 3 40ml / m 3 45ml / m 3 and any point value within its range.

[0031] In a preferred embodiment, the carbon-nitrogen ratio of the fermented feed is 10:1, which can meet the nutritional needs of sea cucumber seedlings under this ratio. The fermented feed is prepared by the following method: adding 1% brewer's yeast and 10% Lactobacillus casei fermentation liquid by weight to the feed, performing semi-solid anaerobic fermentation, and then mixing it with 3 times the weight of dry sea mud to obtain the fermented feed. Specifically, at a temperature of 30°C, adding 1% brewer's yeast and 10% Lactobacillus casei fermentation liquid by weight to the feed, performing semi-solid anaerobic fermentation for 12 hours, and then mixing it with 3 times the weight of dry sea mud to obtain the sea cucumber fermented feed. Among them, the feed is a complete sea cucumber compound feed.

[0032] In a preferred embodiment, the 40th day after the metamorphosis, the young ginseng is peeled off and placed in the cultivation pond for cultivation. The seedling specification is 0.025g / head and the seedling density is 150-200g / m 3 This technical solution limits the seedling density because too high a density will result in smaller seedlings on average, while too low a density will not yield sufficient production capacity. It is understandable that the seedling density can also be 160g / m 3 , 170g / m 3 , 180g / m 3 , 190g / m 3 and any point value within its range.

[0033] In a preferred embodiment, daily feeding of fermented feed and bacterial solution to the target weight includes: changing the water to control the nitrite content in the culture tank to within 0.5 mg / L, feeding the fermented feed and bacterial solution once a day, and the initial feeding amount is 50-60 g / m 3, increase by 10g / m every 7 days 3 , increased to 220g / m 3 No more increase after that.

[0034] In a preferred embodiment, aerobic fermentation of Bacillus subtilis is carried out for 72 hours in clean seawater disinfected at a salinity of 25 to expand to obtain a Bacillus subtilis fermentation liquid; and anaerobic fermentation of Enterococcus faecalis and Lactobacillus casei is carried out for 72 hours in clean seawater disinfected at a salinity of 25 to expand to obtain an Enterococcus faecalis fermentation liquid and a Lactobacillus casei fermentation liquid.

[0035] In a preferred embodiment, the fermented feed and bacterial liquid are continuously sprayed for 15 days until the water in the cultivation tank is clear and transparent, the feed on the corrugated plate is fluffy and short and thick, and a stable beneficial bacteria phase is formed. The water is changed to reduce nitrite to within 0.02 mg / L.

[0036] In a preferred embodiment, the bottom of the sea cucumber cement cultivation pond is covered with a polyethylene corrugated board attachment base used for sea cucumber seedlings, and the corrugated board is placed parallel to the pond bottom. After placement, the cement pond is filled with clean seawater and inflated, and the water temperature is controlled at 22°C.

[0037] In a preferred embodiment, the step of peeling off the young ginseng that has been deformed and attached for 40 days and distributing them into the cultivation pond comprises: stopping the air flow to evenly distribute the young ginseng that has been deformed and attached for 40 days into the cultivation pond, and resuming the inflation after 10 minutes.

[0038] The present invention provides a method for constructing a beneficial bacterial phase for cultivating sea cucumber seedlings, specifically a method for cultivating sea cucumber seedlings in a seawater cultivation pool at a certain temperature, wherein a stable beneficial bacterial phase is formed by using specific organic matter and screened beneficial bacteria to replace antibiotics to inhibit harmful bacteria. Figure 1 As shown, first the sea cucumber cultivation pond after disinfection is filled with polyethylene corrugated plates as sea cucumber seedling attachment base and substrate carrier, the clean seawater after sedimentation and filtration is heated up and filled with the cultivation pond and opened for inflation, then the specific formula feed is subjected to anaerobically fermented, the fermented feed is mixed with the good beneficial bacteria screened every day and fed, after about 15 days of continuous feeding, the feed accumulated on the corrugated plates in the pond is progressively enzymolyzed under the action of bacteria, has become the substrate of beneficial bacteria, the water is also full of a large amount of beneficial bacteria, so far the beneficial bacteria phase is constructed and completed, now put into healthy sea cucumber seedlings and normally manage, and do not need any disinfectant or antibiotic to control harmful bacteria in the process. In this environment, there are sufficient substrates to supply beneficial bacteria for breeding, and the sea cucumber seedlings can grow rapidly and uninterruptedly before the substrate is consumed. This method can effectively prevent the sea cucumber seedlings from being infected by harmful bacteria, reduce the use of antibiotics and disinfectants, ensure the smooth implementation of the seedling production plan, and promote the healthy and sustainable development of the sea cucumber breeding industry.

[0039] The present invention provides a scientific and efficient method for constructing a green and antibiotic-free rapid growth environment for sea cucumber seedlings, which can achieve high-speed growth in a relatively short period of time, effectively solves the benign relationship between feed and beneficial bacteria, establishes a stable beneficial bacteria phase water system, does not use any antibiotics and disinfectants, and promotes the healthy and sustainable development of the sea cucumber seedling industry.

[0040] In order to more clearly and in detail introduce the sea cucumber seedling cultivation method provided by the embodiment of the present invention, it will be described below in conjunction with specific embodiments.

[0041] Example 1

[0042] According to the production plan, fertilized sea cucumber eggs were purchased in April and seedling cultivation began. Preparation of the beneficial bacteria fermentation broth began 22 days after the larvae metamorphosed and settled. This was expanded in disinfected, clean seawater at a salinity of 25°C. The fermentation method used was aerobic fermentation with Bacillus subtilis and anaerobic fermentation with Enterococcus faecalis and Lactobacillus casei. The fermentation temperature and nutrients were provided as needed, and the fermentation lasted for 72 hours. On the morning of the 25th day after the larvae metamorphosed and settled, 600 grams of complete sea cucumber feed was weighed, added to 6 grams of brewer's yeast powder, 6 grams of glucose, 60 milliliters of Lactobacillus casei fermentation broth, and 2000 milliliters of clean seawater. The mixture was stirred thoroughly, then sealed and fermented at 30°C for 12 hours.

[0043] The cement cultivation pond at the nursery in this example measured 3m*6m*1m. On the 24th day after the juveniles had metamorphosed and settled, the bottom of the cultivation pond was covered with 120 sets of polyethylene corrugated sheets for sea cucumber seedlings. Each set contained 20 sheets, for a total of 2,400 sheets. The sheets were placed parallel to the pond bottom, with no gaps between each set. After placement, the cement pond was filled with clean seawater and aerated to a suitable temperature of approximately 22°C.

[0044] On the evening of the 25th day after the larvae metamorphose and settle, mix the 12-hour-fermented feed with 1800 grams of dry sea mud, add 40 ml of Bacillus subtilis fermentation broth and 900 ml of Enterococcus faecalis fermentation broth, and stir thoroughly with an appropriate amount of clean seawater. Sprinkle the mixture evenly throughout the prepared cement culture tank. Apply the mixture daily for 15 consecutive days without changing the water. After 15 days, the water will be clear and translucent, with a uniform layer of short, coarse, and fluffy feed covering the corrugated plate. Monitoring with TCBS medium will reveal the absence of Vibrio, indicating the establishment of a stable beneficial bacterial population. Change the water as needed to reduce nitrite levels to below 0.02 mg / l.

[0045] Forty days after metamorphosis, healthy juvenile ginseng are separated and evenly distributed in a cultivation tank that has been primed with beneficial bacteria and a basic feed. The average seedling size at this point is 0.025 grams per head, and the total weight of the seedlings in this cement cultivation tank is 3,000 grams. When placing the seedlings, turn off the aeration system and evenly distribute the seedlings. Restore aeration 10 minutes after distribution. Disinfection, medicated baths, or antibiotics are not required. Thereafter, the water temperature for the ginseng seedlings is maintained at around 22°C, and the water is changed every five days. The amount of water changed is determined by nitrite levels, keeping it below 0.5 mg / L. Feed the above mixture once daily, starting with 1,000 grams of feed. Increase by 200 grams every seven days until 4,000 grams are reached and no more increases are made. Allow the seedlings to consume the feed on the corrugated plate.

[0046] After about 30 days of management, the feed on the corrugated plate has been consumed by the sea cucumber seedlings, or very little remains. At this point, the average size of the sea cucumber seedlings has reached approximately 0.625 grams per head, and their weight has grown to 75,000 grams, with a 100% survival rate. At this point, the seedlings have reached marketable size and are ready for stocking in small cages or ponds for further cultivation. This process maintains stable water quality, eliminates harmful bacteria, and uses no antibiotics, resulting in uninterrupted and rapid growth. Ultimately, the seedlings weigh approximately 25 times their initial stocking weight.

[0047] Comparative Example 1

[0048] According to the production plan, fertilized sea cucumber eggs were purchased in April to start seedling cultivation, and preparations for peeling and dividing the seedlings began 38 days after the larvae metamorphosed and attached.

[0049] The cement cultivation pond at the nursery of this embodiment is 3m*6m*1m in size. On the 24th day after the juveniles metamorphosed and attached, the bottom of the cultivation pond was covered with a polyethylene corrugated board substrate used for the sea cucumber seedlings. The number was 120 sets, each containing 20 corrugated boards, totaling 2400 corrugated boards. The corrugated boards were placed parallel to the pond bottom, and placed closely together without leaving a gap between each two sets. After placement, the cement pond was filled with clean seawater, sprinkled with 10ppm5% chlorine dioxide disinfectant, and appropriately aerated. The water temperature was controlled at about 22°C.

[0050] Forty days after metamorphosis, healthy juvenile ginseng are removed and evenly distributed in a cement-lined cultivation tank. The average seedling size at this point is 0.025 grams per head, and the total weight of the seedlings in this cement cultivation tank is 3,000 grams. When distributing the seedlings, the aeration system is turned off and the seedlings are evenly distributed. After 10 minutes, aeration is resumed and 90 grams of 30% florfenicol is applied as a disease prevention solution. Thereafter, the water temperature for the seedling cultivation is maintained at around 22°C, and the water is changed every five days. The amount of water changed is determined by nitrite levels, which are kept below 0.5 mg / L. Feed is administered once daily, starting with 1,000 grams of feed, adjusted based on actual feeding habits, and 90 grams of oxytetracycline hydrochloride is mixed into the feed with each feeding. After about 30 days of feeding, the feed on the corrugated plate has been consumed by the sea cucumbers, or very little remains. The average size of the sea cucumbers has reached approximately 0.465 grams per head, and their weight has grown to 46,500 grams, with a survival rate of 83.3%. The sea cucumbers have reached marketable size and are ready for stocking in small cages or ponds for further culture. During this process, 90 grams of florfenicol and 2,700 grams of oxytetracycline hydrochloride are added, resulting in a final weight of approximately 15.5 times the initial weight.

[0051] Comparative Example 2

[0052] The method is the same as Example 1, except that on the 35th day after the juveniles metamorphose and attach, the healthy juvenile ginseng are peeled off and evenly placed in a cultivation pond where a beneficial bacteria phase and a bait base have been formed.

[0053] After about 30 days of management since the seedlings were released, the feed on the corrugated board had been eaten by the sea cucumber seedlings or very little remained. At this time, the average size of the sea cucumber seedlings had reached about 0.411 grams per head, the weight had increased to 41,100 grams, and the survival rate was 81.5%.

[0054] Comparative Example 3

[0055] The method is the same as Example 1, except that on the 45th day after the juveniles metamorphose and attach, the healthy juvenile ginseng are peeled off and evenly placed in a cultivation pond where a beneficial bacteria phase and a bait base have been formed.

[0056] After about 30 days of management since the seedlings were released, the feed on the corrugated board had been eaten by the sea cucumber seedlings or very little remained. At this time, the average size of the sea cucumber seedlings had reached about 0.726 grams per head, the weight had increased to 72,600 grams, and the survival rate was 85.2%.

[0057] Comparative Example 4

[0058] According to the production plan, fertilized sea cucumber eggs were purchased in April and seedling cultivation began. Preparation of the beneficial bacteria fermentation broth began 22 days after the larvae metamorphosed and settled. This was expanded in disinfected, clean seawater at a salinity of 25°C. The fermentation method used was aerobic fermentation with Bacillus subtilis and anaerobic fermentation with Enterococcus faecalis and Lactobacillus casei. The fermentation temperature and nutrients were provided as needed, and the fermentation lasted for 72 hours. On the morning of the 25th day after the larvae metamorphosed and settled, 600 grams of complete sea cucumber feed was weighed, added to 6 grams of brewer's yeast powder, 6 grams of glucose, 60 milliliters of Lactobacillus casei fermentation broth, and 2000 milliliters of clean seawater. The mixture was stirred thoroughly, then sealed and fermented at 30°C for 12 hours.

[0059] The cement cultivation pond at the nursery in this example measured 3m*6m*1m. On the 24th day after the juveniles had metamorphosed and settled, the bottom of the cultivation pond was covered with 120 sets of polyethylene corrugated sheets for sea cucumber seedlings. Each set contained 20 sheets, for a total of 2,400 sheets. The sheets were placed parallel to the pond bottom, with no gaps between each set. After placement, the cement pond was filled with clean seawater and aerated to a suitable temperature of approximately 22°C.

[0060] On the 40th day after the juveniles metamorphosed and attached, the healthy juvenile ginseng were peeled off and evenly placed in the cultivation pond. At this time, the average size of the seedlings was 0.025 grams per head. The total weight of the ginseng seedlings in this cement cultivation pond was 3,000 grams. When placing them, stop the inflation first, sprinkle the ginseng seedlings evenly, and resume inflation 10 minutes after sprinkling.

[0061] Mix the 12-hour fermented feed with 1800 grams of dry sea mud and add an appropriate amount of clean seawater to create a mixture. Maintain the water temperature for ginseng seedling cultivation at around 22°C, changing the water every five days. The amount of water changed depends on the nitrite content, keeping it below 0.5 mg / L. Feed the mixture once daily, starting with 1000 grams. Increase by 200 grams every seven days until you reach 4000 grams, and then stop increasing. Wait until the feed on the corrugated plate is consumed.

[0062] After about 30 days of management since the seedlings were released, the feed on the corrugated board had been eaten by the sea cucumber seedlings or very little remained. At this time, the average size of the sea cucumber seedlings had reached about 0.256 grams per head, the weight had increased to 12,500 grams, and the survival rate was 50.6%.

Claims

1. A method for cultivating sea cucumber seedlings, characterized in that: include: Sprinkling fermented feed containing Bacillus subtilis fermentation liquid and bacterial liquid containing Enterococcus faecalis fermentation liquid and Lactobacillus casei fermentation liquid into a cultivation pool whose bottom is covered with a polyethylene corrugated board attachment base; Continuously sprinkle fermented feed and bacterial liquid until the water in the cultivation pool is clear and bright, the feed on the corrugated plate is fluffy and short and thick, and a stable beneficial bacteria phase is formed. Change the water to reduce nitrite to less than 0.02mg / L; The young ginseng on the 40th day of metamorphosis was peeled off and placed in the cultivation pond for cultivation, and was fed with fermented feed and bacterial liquid every day.

2. The method for cultivating sea cucumber seedlings according to claim 1, wherein The strain of Bacillus subtilis is bio-67384, with a deposit number of CMCC(B)63501; the strain of Enterococcus faecalis is bio-53874, with a deposit number of CMCC(B)32482; and the strain of Lactobacillus casei is bio-67414, with a deposit number of CGMCC NO.15409.

3. The method for cultivating sea cucumber seedlings according to claim 1, wherein The dosage of Bacillus subtilis fermentation broth is 2-2.5ml / m 3 The dosage of Enterococcus faecalis fermentation liquid is 30-50ml / m 3 .

4. The method for cultivating sea cucumber seedlings according to claim 1, wherein The carbon-nitrogen ratio of the fermented feed is 10:1; The fermented feed is prepared by the following method: adding 1% of brewer's yeast and 10% of lactobacillus casei fermentation liquid to the feed weight, performing semi-solid anaerobic fermentation, and then uniformly mixing with 3 times the weight of dry sea mud to obtain the fermented feed.

5. The method for cultivating sea cucumber seedlings according to claim 1, wherein The young ginseng on the 40th day of metamorphosis was peeled off and placed in the cultivation pond for cultivation. The seedling specification was 0.025g / head and the seedling density was 150-200g / m 3 .

6. The method for cultivating sea cucumber seedlings according to claim 1, wherein Feeding fermented feed and bacterial liquid daily to target body weight includes: Change the water to control the nitrite content in the culture tank within 0.5mg / L, feed the fermented feed and bacterial solution once a day, and the initial feeding amount is 50-60g / m 3 , increase by 10g / m every 7 days 3 , increased to 220g / m 3 No more increase after that.

7. The method for cultivating sea cucumber seedlings according to claim 1, wherein Bacillus subtilis was aerobic fermented for 72 hours in clean seawater after disinfection at a salinity of 25, and Bacillus subtilis fermentation liquid was obtained by expansion; Enterococcus faecalis and Lactobacillus casei were anaerobic fermented for 72 hours in clean seawater after disinfection at a salinity of 25, and Enterococcus faecalis fermentation liquid and Lactobacillus casei fermentation liquid were obtained by expansion.

8. The method for cultivating sea cucumber seedlings according to claim 1, wherein Sprinkle the fermented feed and bacterial liquid continuously for 15 days until the water in the culture pond is clear and transparent, the feed on the corrugated plate is fluffy and short and thick, and a stable beneficial bacteria phase is formed. Change the water to reduce nitrite to less than 0.02 mg / L.

9. The method for cultivating sea cucumber seedlings according to claim 1, wherein The bottom of the sea cucumber cement culture pond is covered with polyethylene corrugated board attachment base used for sea cucumber seedlings. The corrugated board is placed parallel to the pond bottom. After placement, the cement pond is filled with clean seawater and inflated. The water temperature is controlled at 22°C.

10. The method for cultivating sea cucumber seedlings according to claim 1, wherein The method of peeling off the young ginseng that has been attached to the culture pond for 40 days after metamorphosis and distributing them into the culture pond includes: stopping the air flow to evenly distribute the young ginseng that has been attached to the culture pond for 40 days after metamorphosis, and resuming the air flow after 10 minutes.

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