Microbial floc ectopic culture-based shrimp seed thickening device and method

Abstract

The invention belongs to the technical field of aquaculture, and particularly relates to a shrimp seed thickness marking device and method based on microbial floc ectopic culture, the device comprises a seed marking pond, a heterotrophic microorganism purification pond and a carbon source adding device, the interior of a porous escape-proof vertical pipe is communicated with a gravity self-flowing drainage pipe; the water flows into the floc anti-escape screen frame of the heterotrophic microorganism purification tank through the gravity self-flowing drainage pipe, the bottom between the purification tank body and the inner floc anti-escape screen frame is communicated with a circulating pump water inlet pipe, the water flows into the standard seedling tank body through a circulating pump, and the carbon source adding device is communicated with the interior of the floc anti-escape screen frame and adds a carbon source solution into the floc anti-escape screen frame. According to the method, automatic culture and management of cenobium biomass can be realized according to daily feeding conditions and brush cleaning frequency, and the water quality of a standard seedling pond is controlled at a safe concentration.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture, and in particular relates to a shrimp seed marking device and method based on ectopic culture of microbial flocs. Background Art

[0002] The coarsening process for shrimp seedlings mainly uses different techniques such as algal culture, floc culture (mixed phase), bacterial culture, and water exchange culture. If the first three culture techniques are properly controlled, water consumption can be greatly reduced, and the coarsening process can even be completed without water changes. In recent years, the development of artificial seawater aquaculture of marine shrimp in inland areas of my country has increased, and production problems have also arisen. Especially in inland plateau areas such as Yunnan and Guizhou, due to the low oxygen content in the air and the high cost of preparing artificial seawater, how to efficiently cultivate shrimp seedlings with little or no water changes and complete the coarsening stage of aquaculture is a great challenge.

[0003] Cultivating shrimp seedlings using algal or mixed-bacteria-algal flocculent cultures is susceptible to climatic conditions such as cloudy days. Continuous cloudy days can easily cause algae death, which in turn produces algal toxins that affect shrimp survival. Water exchange aquaculture not only consumes high heating energy and water costs, but also increases water costs. Therefore, using a bacterial culture model for shrimp seedling standardization has become the preferred method for reducing costs and increasing efficiency.

[0004] The traditional bacterial biofloc culture method is to add a variety of ingredients such as carbon sources, heterotrophic bacteria, feed, vitamins, etc. in a reasonable ratio to the water body in which the shrimp fry are cultivated, so as to maintain the nutrients and ammonia nitrogen, nitrite, etc. in the water body at a healthy level. The risk of the traditional bacterial biofloc culture method is that with the rapid growth of heterotrophic microbial flocs, the oxygen consumption of the water body will increase rapidly, making it difficult for the dissolved oxygen level in the water bodies in plateau areas with low dissolved oxygen levels (saturated dissolved oxygen <5.5mg / L) to remain in the range suitable for the growth of shrimp fry. As the biomass increases, the oxygen-consuming organic matter in the water also increases, which limits the density of shrimp fry coarse stocking and forces the coarse stocking process to be terminated prematurely, affecting the overall production efficiency of the aquaculture. Summary of the Invention

[0005] In order to solve the above-mentioned problems existing in the traditional microbial floc culture method in the shrimp seed standardization process, the purpose of the present invention is to provide a shrimp seed standardization device and method based on the ectopic culture of microbial flocs.

[0006] The object of the present invention is achieved through the following technical solutions:

[0007] The shrimp seed marking device of the present invention comprises a seed marking pool, a heterotrophic microorganism purification pool and a carbon source adding device;

[0008] The standard seedling pond includes an automatic feeding machine, a pressure water pump, a self-movable flushing pipe, an anti-escape net A, a porous anti-escape riser, an aeration plate A, a standard seedling pond body, a sewage valve and a gravity self-flowing drainage pipe. The porous anti-escape riser is installed in the standard seedling pond body, the anti-escape net A is coated on the outside of the porous anti-escape riser, and the porous anti-escape riser coated with the anti-escape net A is provided with a self-movable flushing pipe that can be relatively lifted and lowered to flush the anti-escape net A and the porous anti-escape riser, and the self-movable flushing pipe is connected to the water source through a pressure water pump; an aeration plate A is provided at the bottom of the standard seedling pond body, located on the periphery of the porous anti-escape riser; an automatic feeding machine is provided above the standard seedling pond body, and the interior of the porous anti-escape riser is connected to the gravity self-flowing drainage pipe;

[0009] The heterotrophic microbial purification tank includes a circulation pump, a variable frequency motor, a scrubbing device, a floc collection pipe, an aeration plate B, a floc anti-escape net frame and a purification tank body. The floc anti-escape net frame is arranged in the purification tank body. The floc anti-escape net frame is provided with an anti-escape net B for intercepting flocs in the aquaculture water body. The interior of the porous anti-escape riser is connected to the interior of the floc anti-escape net frame through a gravity self-flow drainage pipe. The purification tank body is installed with a variable frequency motor. The output end of the variable frequency motor is connected to the scrubbing device located in the floc anti-escape net frame to drive the scrubbing device to scrub the floc anti-escape net frame; the bottom of the floc anti-escape net frame is provided with an aeration plate B, and excess biological flocs are collected through the floc collection pipe connected to the bottom of the floc anti-escape net frame; the circulation pump water inlet pipe of the circulation pump is connected to the interior of the purification tank body, and the circulation pump water outlet pipe of the circulation pump is connected to the interior of the standard seedling tank body;

[0010] The carbon source adding device is connected to the interior of the floc anti-escape net frame, and adds the carbon source solution into the floc anti-escape net frame.

[0011] Among them: the self-movable flushing pipe is divided into two groups, an upper and lower group, which are connected by connecting pipes. Each group includes a flushing pipe, a stainless steel ring and a ring-shaped brush A. The outer ring of the flushing pipe is connected to the stainless steel ring serving as a counterweight, and the inner ring of the flushing pipe is connected to the brush A. The upper and lower groups of flushing pipes are connected by multiple connecting pipes; the bottom surface of the flushing pipe is evenly provided with multiple water outlet holes along the circumferential direction, and the axial center line of the water outlet is inclined to the axial center line of the flushing pipe, and the flushing pipe located above is connected to the pressure water pump.

[0012] The carbon source adding device includes a carbon source solution adding pump, a carbon source adding device and a carbon source storage tank. The carbon source storage tank is filled with seawater. The carbon source adding device is provided above the carbon source storage tank. The carbon source solution adding pump water inlet pipe of the carbon source solution adding pump is connected to the interior of the carbon source storage tank. The carbon source solution adding pump water outlet pipe of the carbon source solution adding pump is connected to the interior of the floc escape prevention net frame.

[0013] An aeration plate C is provided at the bottom of the carbon source storage tank to promote the dissolution of the carbon source. A liquid level sensor is also provided in the carbon source storage tank. The liquid level sensor, carbon source adder, carbon source solution return pump and automatic feeding machine are respectively connected to the controller; a carbon source storage tank water supply pipe is provided on the carbon source storage tank for replenishing seawater into the interior.

[0014] A pressure water pump level switch connected to the pressure water pump is provided in the seedling standard pool. The pressure water pump has two modes: timed start and pressure water pump level switch controlled start.

[0015] A liquid level switch is provided in the purification tank body. The liquid level switch and the variable frequency motor are respectively connected to a controller. The variable frequency motor has two modes: timed start and liquid level switch controlled start.

[0016] The brushing device includes a rotating shaft and a brush B. The rotating shaft is connected to the output end of the frequency conversion motor. Multiple brushes B are axially connected to the rotating shaft. The frequency conversion motor drives the rotating shaft to rotate, thereby driving the multiple brushes B to brush the anti-escape net B of the floc anti-escape net frame.

[0017] One end of the floc collection pipe is connected to the bottom of the floc escape prevention net frame, and the other end of the floc collection pipe is connected to a solenoid valve; the circulating pump water inlet pipe is connected to the bottom of the space between the purification tank body and the floc escape prevention net frame.

[0018] The bottom of the seedling pool body is tilted downward from the outside to the inside, and the gravity drainage pipe is divided into three routes. The first route is connected to the lowest point of the bottom of the seedling pool body, the second route is connected to the inside of the floc escape prevention net frame, and the third route is provided with a sewage valve; the circulating pump outlet pipe is connected to a position near the top of the seedling pool body, and a water supply pipe is also connected to a position near the top of the seedling pool body.

[0019] The method for using the shrimp seed marking device based on the ectopic culture of microbial flocs of the present invention comprises the following steps:

[0020] Step A, before the seedlings are introduced, water is added to the purification tank to a set water level, probiotics and a carbon source are added to the floc escape prevention net frame, and aeration is performed through the aeration plate B to allow the flocs to grow and form granular flocs;

[0021] Step B, after the shrimp fry are placed in the standard seedling pond, the shrimp fry are fed by the automatic feeding machine, the circulation pump is turned on, the water between the floc escape prevention net frame and the purification tank body is pumped into the standard seedling pond body, and the water in the porous escape prevention standpipe flows into the floc escape prevention net frame through the gravity drainage pipe;

[0022] Step C, when the anti-escape net A on the porous anti-escape standpipe is blocked and causes the water level in the standard seedling pool to rise, the pressure water pump is manually or automatically started to pump water into a self-movable flushing pipe connected to the pressure water pump, and the anti-escape net A and the porous anti-escape standpipe are cleaned of dirt by the up and down movement of the self-movable flushing pipe;

[0023] Step D, according to the daily feeding amount of the automatic feeding machine, turning on the scrubbing device by the variable frequency motor at a set time interval;

[0024] Step E, adding a carbon source solution into the floc escape prevention net frame according to the daily feeding amount of the automatic feeding machine;

[0025] Step F, controlling the biomass of the flocs, evaluating the biomass of the flocs by manual inspection or the frequency of cleaning the floc escape prevention net frame, collecting excess bioflocs using the floc collection pipe, and collecting the discharged bioflocs by settling and collecting them by filter pressing for use as feed additives or biofertilizers.

[0026] The advantages and positive effects of the present invention are:

[0027] 1. The present invention has a high degree of automation and realizes the recycling of water through a circulating pump, which saves more than 90% of water compared with the traditional seedling standard method and greatly reduces heating energy consumption.

[0028] 2. The aquaculture tail water of the present invention is purified in situ and no aquaculture tail water is discharged.

[0029] 3. The biofloc produced by the present invention is a good probiotic and can be used for regulating the aquaculture water environment or as an auxiliary material for adding protein to feed. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a schematic diagram of the overall structure of the shrimp fry marking device of the present invention;

[0031] Figure 2 It is a bottom view of the structure of the self-movable flushing pipe and the porous escape-proof riser in the shrimp fry marking device of the present invention;

[0032] Figure 3 This is a structural front view of the self-movable flushing pipe and the porous escape-proof riser in the shrimp fry marking device of the present invention;

[0033] Wherein: 1 is the standard seedling pond, 101 is the automatic feeding machine, 102 is the pressure water pump, 103 is the pressure water pump inlet pipe, 104 is the self-moving flushing pipe, 1041 is the flushing pipe, 1042 is the water outlet, 1043 is the stainless steel ring, 1044 is the brush, 1045 is the rubber bottom of the brush, 1046 is the connecting pipe, 105 is the water supply pipe, 106 is the escape prevention net A, 107 is the multi-hole escape prevention standpipe, 108 is the point-distributed aeration disk A, 109 is the standard seedling pond body, 110 is the sewage valve, 111 is the gravity self-flowing drainage pipe, 112 is the pressure water pump level switch, 113 is the inflation pipe A;

[0034] 2 is a heterotrophic microbial purification tank, 201 is a circulation pump, 202 is a circulation pump water inlet pipe, 203 is a circulation pump water outlet pipe, 204 is a frequency conversion motor, 205 is a liquid level switch, 206 is a fixing bracket, 207 is a brush B, 208 is a solenoid valve, 209 is a rotating shaft, 210 is a floc collection pipe, 211 is an aeration pipe B, 212 is a point-distributed aeration disk B, 213 is a floc escape prevention net frame, and 214 is a purification tank body;

[0035] 3 is a carbon source adding device, 301 is a controller, 302 is a carbon source solution adding pump, 303 is a carbon source adding device, 304 is a water inlet pipe of the carbon source solution adding pump, 305 is a liquid level sensor, 306 is a carbon source solution adding pipe, 307 is a water supply pipe for the carbon source storage tank, 308 is a carbon source storage tank, and 309 is a point-distributed aeration plate C. DETAILED DESCRIPTION

[0036] The present invention will be further described below in conjunction with the accompanying drawings.

[0037] like Figure 1 As shown, the shrimp seed marking device of the present invention includes a seed marking pool 1, a heterotrophic microorganism purification pool 2 and a carbon source adding device 3.

[0038] The seedling pond 1 includes an automatic feeding machine 101, a pressure water pump 102, a self-moving flushing pipe 104, an anti-escape net A106, a porous anti-escape standpipe 107, an aeration plate A, a seedling pond body 109, a sewage valve 110 and a gravity self-flowing drainage pipe 111. The porous anti-escape standpipe 107 is installed in the seedling pond body 109, the anti-escape net A106 is coated on the porous anti-escape standpipe 107, and the anti-escape net A106 is coated on the porous anti-escape standpipe 107. It is equipped with a self-moving flushing pipe 104 which can be raised and lowered relative to each other to flush the anti-escape net A106 and the porous anti-escape riser 107. The self-moving flushing pipe 104 is connected to the water source through a pressure water pump 102; an aeration plate A is provided at the bottom of the seedling pool body 109, located on the periphery of the porous anti-escape riser 107; an automatic feeding machine 101 is provided above the seedling pool body 109, and a gravity drainage pipe 111 is connected to the interior of the porous anti-escape riser 107.

[0039] The heterotrophic microbial purification tank 2 includes a circulation pump 201, a variable frequency motor 204, a scrubbing device, a floc collection pipe 210, an aeration plate B, a floc escape prevention net frame 213 and a purification tank body 214. The floc escape prevention net frame 213 is arranged in the purification tank body 214. The floc escape prevention net frame 213 is provided with an escape prevention net B for intercepting flocs in the aquaculture water body. The interior of the porous escape prevention standpipe 107 is connected to the interior of the floc escape prevention net frame 213 through a gravity self-flow drainage pipe 111. The purification tank body 214 is equipped with a variable frequency motor 204. The output end of the variable frequency motor 204 is connected to the floc escape prevention net frame 213. The brushing device inside is connected to the brushing device and drives the brushing device to brush the floc anti-escape frame 213; an aeration plate B is provided at the bottom of the floc anti-escape frame 213, and excess biological flocs are collected through a floc collection pipe 210 connected to the bottom of the floc anti-escape frame 213; the circulating pump water inlet pipe 202 of the circulating pump 201 is connected to the inside of the purification tank body 214, specifically, it is connected to the bottom of the space between the purification tank body 214 and the floc anti-escape frame 213, and the circulating pump water outlet pipe 203 of the circulating pump 201 is connected to the inside of the standard seedling tank body 109, and water flows through the circulating pump 201 and is sent into the standard seedling tank body 109.

[0040] The carbon source adding device 3 includes a carbon source solution adding pump 302, a carbon source adding device 303 and a carbon source storage tank 308. The carbon source storage tank 308 is filled with seawater. The carbon source adding device 303 is provided above the carbon source storage tank 308. The carbon source solution adding pump water inlet pipe 304 of the carbon source solution adding pump 302 is connected to the interior of the carbon source storage tank 308. The carbon source solution adding pump water outlet pipe 306 of the carbon source solution adding pump 302 is connected to the interior of the floc anti-escape net frame 213, and the carbon source solution is added to the floc anti-escape net frame 213.

[0041] The porous anti-escape riser 107 of this embodiment is a hollow circular tube with openings at both ends, and a plurality of hollow holes are provided on the circular tube along the circumferential direction; the outer side of the hollow holes is covered with an anti-escape net A106, and the mesh number of the anti-escape net A06 of this embodiment is 20 meshes.

[0042] like Figures 1 to 3As shown, the self-propelled flushing pipe 104 of this embodiment is divided into two upper and lower groups connected by a connecting pipe 1046. Each group includes a flushing pipe 1041, a stainless steel ring 1043, and a brush A1044. The flushing pipe 1041 is a circular tube made of PVC. The outer ring of the flushing pipe 1041 is connected to the stainless steel ring 1043, which serves as a counterweight. The inner ring of the flushing pipe 1041 is connected to the brush A1044. The brush A1044 of this embodiment is annular. The outer ring of the brush A1044 is a brush bottom rubber 1045, which is fixed to the inner ring of the flushing pipe 1041. The inner ring of the brush A1044 is bristle. The stainless steel ring 1043 of this embodiment weighs 500g. The bottom surface of the flushing pipe 1041 is uniformly circumferentially formed with multiple water outlet holes 1042. The axial centerline of each water outlet hole 1042 is inclined relative to the axial centerline of the flushing pipe 1041. In this embodiment, the angle of inclination is 45°. The diameter of each water outlet hole 1042 is 2 mm, and the spacing between adjacent water outlet holes 1042 is 2-3 mm. The upper flushing pipe 1041 is connected to the pressure water pump 102 for automatic cleaning and smooth water flow. The upper and lower flushing pipes 1041 are connected by multiple connecting pipes 1046.

[0043] In this embodiment, the bottom of the seedling tank body 109 is tilted downward from the outside to the inside, and the gravity drainage pipe 111 is divided into three routes. The first route is connected to the lowest point of the bottom of the seedling tank body 109, the second route is connected to the inside of the floc escape prevention net frame 213, and the third route is provided with a sewage valve 110. The circulating pump outlet pipe 203 is connected to a position near the top of the seedling tank body 109, and the water supply pipe 105 is also connected to the position near the top of the seedling tank body 109.

[0044] In this embodiment, the water inlet of the pressure water pump 102 is connected to the water source through the pressure water pump inlet pipe 103, and the water outlet of the pressure water pump 102 is connected to the flushing pipe 1041 located above through a hose; a pressure water pump liquid level switch 112 connected to the pressure water pump 102 is provided in the seedling pool body 109, and the pressure water pump liquid level switch 112 can be fixed on the seedling pool body 109. The pressure water pump 102 has two modes: timed start and pressure water pump liquid level switch 112 control start. After the pressure water pump 102 is turned on, the self-movable flushing pipe 104 rises under the reaction force of the water outflowing from the water outlet 1042, and cleans the anti-escape net A106 and the porous anti-escape vertical net 107 during the rising process. A cleaning is completed by adjusting the single opening time of the pressure water pump 102. After the cleaning is completed, the self-movable flushing pipe 104 will return to the bottom of the porous anti-escape vertical net 107 under the action of gravity.

[0045] The scrubbing device of this embodiment includes a rotating shaft 209 and brushes B207. A fixed bracket 206 is installed on the top of the purification tank body 214. The variable frequency motor 204 is fixed to the fixed bracket 206. The rotating shaft 209 is connected to the output end of the variable frequency motor 204. Multiple brushes B207 are axially connected to the rotating shaft 209. The variable frequency motor 204 drives the rotating shaft 209 to rotate, thereby driving the multiple brushes B207 to scrub the anti-escape net B of the floc anti-escape net frame 213. The brushes B207 of this embodiment are arranged in a horizontal comb-like shape, that is, each brush B207 is perpendicular to the rotating shaft 209 and has a comb-like outer edge.

[0046] In this embodiment, the anti-escape net B has a mesh size of 100. The flocs in the aquaculture water are trapped by the floc anti-escape net frame 213 and the anti-escape net B, enabling ex situ cultivation of biological flocs. A variable frequency motor 204 drives the rotation of the rotating shaft 209, which in turn drives the rotation of the brushes B207. The brushes B207 clean the floc anti-escape net frame 213 and the anti-escape net B, ensuring smooth water circulation.

[0047] A liquid level switch 205 fixed on a fixed bracket 206 is provided in the purification tank body 214 of this embodiment. The liquid level switch 205 and the frequency conversion motor 204 are respectively connected to the controller 301. The frequency conversion motor 204 has two modes: timed start and liquid level switch 205 controlled start. According to the daily feeding amount of bait of the automatic feeding machine 101, the frequency conversion motor 204 can be turned on at a scheduled time or controlled by the liquid level switch 205 to realize automatic cleaning of the anti-escape net B on the floc anti-escape net frame 213.

[0048] In this embodiment, one end of the floc collection pipe 210 is connected to the bottom of the floc escape prevention net frame 213, and the other end of the floc collection pipe 210 is connected to the solenoid valve 208. Automatic control of floc biomass is achieved by estimating biomass by the activation frequency of the liquid level switch 205 and combining it with the activation of the solenoid valve 208.

[0049] In this embodiment, the bottom of the carbon source storage tank 308 is equipped with an aeration plate C to promote carbon source dissolution. The carbon source storage tank 308 is also equipped with a liquid level sensor 305. The liquid level sensor 305, carbon source feeder 303, carbon source solution refill pump 302, and automatic feeding machine 101 are each connected to the controller 301. A carbon source storage tank refill pipe 307 is provided on the carbon source storage tank 308 for replenishing seawater. Automatic allocation of the carbon source solution is achieved by sensing the remaining water level through the liquid level sensor 205 and automatically replenishing water through the refill pipe 307, in conjunction with the carbon source feeder 303. Automatic replenishment of the carbon source solution is achieved by estimating the floc biomass by the activation frequency of the variable frequency motor 204 and, in combination with the daily feeding amount recorded by the automatic feeding machine 101, controlling the carbon source solution refill pump 302 via the controller 301 to replenish the carbon source solution to the purification tank 214. The carbon source solution is automatically replenished by adding 50-80% of the daily feed mass of the automatic feeding machine 101. Under the same feeding conditions, the amount of carbon source solution added increases as the frequency of the variable frequency motor 204 increases. In this embodiment, the carbon source can be glucose, sucrose, or molasses. The carbon source storage tank 308 contains 400 L of seawater, and the carbon source mass ratio is 100 g of carbon source / L of water.

[0050] In this embodiment, aeration plate A is a point-distributed aeration plate A108, aeration plate B is a point-distributed aeration plate B212, and aeration plate C is a point-distributed aeration plate C309. The carbon source feeder 303 in this embodiment is conventional technology and can be a motor and an auger to add carbon source to the carbon source storage tank 308.

[0051] The method for using the shrimp seed marking device based on the ectopic culture of microbial flocs of the present invention comprises the following steps:

[0052] Step A: Three days before seedlings are introduced, water is added to the purification tank 214 to a set water level, probiotics and a carbon source are added to the floc escape prevention net frame 213, and aeration is performed through the aeration plate B to rapidly grow the flocs to form granular flocs; the probiotics can be Bacillus or EM bacteria, 30 to 50 g of probiotics are added each time, and 50 to 80% of the daily feeding mass of the carbon source is added to the automatic feeding machine 101;

[0053] Step B: After the shrimp fry are placed in the seedling standard pond body 109, the shrimp fry are fed by the automatic feeding machine 101, the circulation pump 201 is turned on, and the water between the floc anti-escape net frame 213 and the purification tank body 214 is pumped into the seedling standard pond body 109. The water in the porous anti-escape standpipe 107 flows into the floc anti-escape net frame 213 through the gravity drainage pipe 111;

[0054] Step C, when the anti-escape net A106 on the porous anti-escape standpipe 107 is blocked and the water level in the standard seedling pond body 109 rises, the pressure water pump 102 is manually or automatically started to pump water into the self-movable flushing pipe 104 connected to the pressure water pump 102, and the dirt on the anti-escape net A106 and the porous anti-escape standpipe 107 is cleaned by the up and down movement of the self-movable flushing pipe 104;

[0055] Step D, according to the daily feeding amount of the automatic feeding machine 101, the brush B207 is turned on by the frequency conversion motor 204 at a set time interval; the timed opening principle of the frequency conversion motor 204 is based on the working condition of the automatic feeding machine 101, the daily feeding amount is 0-300g, and it is turned on once every 6 hours, the daily feeding amount is 300-600g, and it is turned on once every 3 hours, the daily feeding amount is 600-900g, and it is turned on once every 1 hour, the daily feeding amount is 900-1200g, and it is turned on once every 30 minutes, the daily feeding amount is 1200-1500g, and it is turned on once every 20 minutes, the daily feeding amount is 1500-1800g, and it is turned on once every 10 minutes, and the single opening time is not less than 5 seconds;

[0056] Step E: Adding a carbon source solution to the floc escape prevention net frame 213 according to 50-80% of the daily feeding mass of the automatic feeding machine 101. Under the condition of the same feeding amount, the mass of the carbon source added is increased as the frequency of the variable frequency motor 204 increases;

[0057] Step F, controlling the biomass of the flocs, assessing the biomass of the flocs by manual inspection or by cleaning the floc escape prevention frame 213, collecting excess bioflocs using the floc collection pipe 210, and collecting the discharged bioflocs by settling and collecting them by filter pressing for use as feed additives or biofertilizers.

Claims

1. A shrimp seed marking device based on ectopic culture of microbial flocs, characterized by: It includes a standard seedling pool (1), a heterotrophic microorganism purification pool (2) and a carbon source adding device (3); The seedling pond (1) comprises an automatic feeding machine (101), a pressure water pump (102), a self-moving flushing pipe (104), an anti-escape net A (106), a porous anti-escape standpipe (107), an aeration plate A, a seedling pond body (109), a sewage valve (110) and a gravity self-flow drainage pipe (111), wherein the porous anti-escape standpipe (107) is installed in the seedling pond body (109), the anti-escape net A (106) is coated on the outside of the porous anti-escape standpipe (107), and the porous anti-escape standpipe (107) coated with the anti-escape net A (106) is provided with a drainage valve (110). 7) A self-movable flushing pipe (104) is provided on the upper sleeve and can be raised and lowered relative to flush the anti-escape net A (106) and the porous anti-escape standpipe (107), and the self-movable flushing pipe (104) is connected to a water source via a pressure water pump (102); an aeration plate A is provided at the bottom of the seedling pool body (109) and located on the periphery of the porous anti-escape standpipe (107); an automatic feeding machine (101) is provided above the seedling pool body (109), and a gravity drainage pipe (111) is connected to the interior of the porous anti-escape standpipe (107); The heterotrophic microorganism purification tank (2) comprises a circulation pump (201), a variable frequency motor (204), a scrubbing device, a flocculent collecting pipe (210), an aeration plate B, a flocculent escape prevention net frame (213) and a purification tank body (214). The flocculent escape prevention net frame (213) is arranged in the purification tank body (214). The flocculent escape prevention net frame (213) is provided with an escape prevention net B for intercepting floccules in the aquaculture water body. The interior of the porous escape prevention standpipe (107) is connected to the interior of the flocculent escape prevention net frame (213) through a gravity self-flowing drainage pipe (111). The frequency conversion motor (204) is installed on the purification tank body (214). 04), the output end of the variable frequency motor (204) is connected to a scrubbing device located in a flocculent escape prevention net frame (213), driving the scrubbing device to scrub the flocculent escape prevention net frame (213); an aeration plate B is provided at the bottom of the flocculent escape prevention net frame (213), and excess biological flocs are collected through a floc collection pipe (210) connected to the bottom of the flocculent escape prevention net frame (213); a circulating pump water inlet pipe (202) of the circulating pump (201) is connected to the interior of the purification tank body (214), and a circulating pump water outlet pipe (203) of the circulating pump (201) is connected to the interior of the seedling tank body (109); The carbon source adding device (3) is connected to the interior of the floc escape prevention net frame (213) and adds a carbon source solution into the floc escape prevention net frame (213).

2. The shrimp seed marking device based on ectopic culture of microbial flocs according to claim 1 is characterized in that: The self-movable flushing pipe (104) is divided into two groups, an upper group and an lower group, which are connected by a connecting pipe (1046). Each group includes a flushing pipe (1041), a stainless steel ring (1043) and an annular brush A (1044). The outer ring of the flushing pipe (1041) is connected to the stainless steel ring (1043) serving as a counterweight, and the inner ring of the flushing pipe (1041) is connected to the brush A (1044). The upper and lower groups of flushing pipes (1041) are connected via a plurality of connecting pipes (1046). The bottom surface of the flushing pipe (1041) is evenly provided with a plurality of water outlet holes (1042) along the circumferential direction. The axial center line of the water outlet hole (1042) is inclined with the axial center line of the flushing pipe (1041). The flushing pipe (1041) located at the top is connected to the pressure water pump (102).

3. The shrimp seed marking device based on ectopic culture of microbial flocs according to claim 1 is characterized in that: The carbon source adding device (3) comprises a carbon source solution adding pump (302), a carbon source adding device (303) and a carbon source storage tank (308), wherein the carbon source storage tank (308) is filled with seawater, and a carbon source adding device (303) is provided above the carbon source storage tank (308), a carbon source solution adding pump water inlet pipe (304) of the carbon source solution adding pump (302) is connected to the interior of the carbon source storage tank (308), and a carbon source solution adding pump water outlet pipe (306) of the carbon source solution adding pump (302) is connected to the interior of the floc escape prevention net frame (213).

4. The shrimp seed marking device based on ectopic culture of microbial flocs according to claim 3 is characterized in that: An aeration plate C for promoting carbon source dissolution is provided at the bottom of the carbon source storage tank (308). A liquid level sensor (305) is also provided in the carbon source storage tank (308). The liquid level sensor (305), the carbon source additive (303), the carbon source solution return pump (302) and the automatic feeding machine (101) are respectively connected to the controller (301). The carbon source storage tank (308) is provided with a carbon source storage tank water supply pipe (307) for replenishing seawater into the interior.

5. The shrimp seed marking device based on ectopic culture of microbial flocs according to claim 1 is characterized in that: A pressure water pump level switch (112) connected to the pressure water pump (102) is provided in the seedling marking pool body (109). The pressure water pump (102) has two modes: timed start and start controlled by the pressure water pump level switch (112).

6. The shrimp seed marking device based on ectopic culture of microbial flocs according to claim 1 is characterized in that: A liquid level switch (205) is provided in the purification tank body (214). The liquid level switch (205) and the variable frequency motor (204) are respectively connected to the controller (301). The variable frequency motor (204) has two modes: timed start and liquid level switch (205) controlled start.

7. The shrimp seed marking device based on ectopic culture of microbial flocs according to claim 1 is characterized in that: The scrubbing device comprises a rotating shaft (209) and a brush B (207). The rotating shaft (209) is connected to the output end of the variable frequency motor (204). A plurality of brushes B (207) are axially connected to the rotating shaft (209). The variable frequency motor (204) drives the rotating shaft (209) to rotate, thereby driving the plurality of brushes B (207) to scrub the anti-escape net B of the floc anti-escape net frame (213).

8. The shrimp seed marking device based on ectopic culture of microbial flocs according to claim 1, characterized in that: One end of the floc collection pipe (210) is connected to the bottom of the floc escape prevention net frame (213), and the other end of the floc collection pipe (210) is connected to a solenoid valve (208); the circulating pump water inlet pipe (202) is connected to the bottom of the space between the purification tank body (214) and the floc escape prevention net frame (213).

9. The shrimp seed marking device based on ectopic culture of microbial flocs according to claim 1, characterized in that: The bottom of the seedling pool (109) is tilted downward from the outside to the inside, and the gravity drainage pipe (111) is divided into three routes, the first route is connected to the lowest point of the bottom of the seedling pool (109), the second route is connected to the inside of the floc escape prevention net frame (213), and the third route is provided with a sewage valve (110); the circulating pump outlet pipe (203) is connected to a position near the top of the seedling pool (109), and a water supply pipe (105) is also connected to a position near the top of the seedling pool (109).

10. A method for using the shrimp seed marking device based on ex situ culture of microbial flocs according to any one of claims 1 to 9, characterized in that: Includes the following steps Step A, before the seedlings are introduced, water is added to the purification tank (214) to a set water level, probiotics and a carbon source are added to the floc escape prevention net frame (213), and aeration is performed through the aeration plate B to allow the flocs to grow and form granular flocs; Step B, after the shrimp fry are placed in the seedling standard pond body (109), the shrimp fry are fed by the automatic feeding machine (101), the circulation pump (201) is turned on, and the water between the flocculent escape prevention net frame (213) and the purification tank body (214) is pumped into the seedling standard pond body (109), and the water in the porous escape prevention standpipe (107) flows into the flocculent escape prevention net frame (213) through the gravity drainage pipe (111); Step C, when the anti-escape net A (106) on the porous anti-escape standpipe (107) is blocked and causes the water level in the seedling pool (109) to rise, the pressure water pump (102) is manually or automatically turned on, and water is pumped into a self-movable flushing pipe (104) connected to the pressure water pump (102), and the anti-escape net A (106) and the porous anti-escape standpipe (107) are cleaned by moving the self-movable flushing pipe (104) up and down; Step D, according to the daily feeding amount of the automatic feeding machine (101), the brushing device is turned on by the variable frequency motor (204) at a set time interval; Step E, adding a carbon source solution into the floc escape prevention net frame (213) according to the daily feeding amount of the automatic feeding machine (101); Step F, controlling the biomass of the flocs, evaluating the biomass of the flocs by manual inspection or the frequency of cleaning the floc escape prevention net frame (213), collecting excess bioflocs using the floc collection pipe (210), and collecting the discharged bioflocs by settling and collecting them, and then collecting them by filter pressing for use as feed additives or biofertilizers.

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