Toning lotion with rapid moisturizing, tightening and soothing effects and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses a toning lotion with rapid moisturizing, firming and soothing effects and a preparation method thereof.The toning lotion takes embryo extract in a specific proportion as a core and cooperates with glycerin and propylene glycol to construct a rapid moisture absorption-water locking network, panthenol and copper gluconate are matched to enhance the firming and anti-wrinkle effects, and the toning lotion has the advantages of being good in moisturizing, firming and soothing effects and good in moisturizing, firming and soothing effects. Plant extracts such as chrysanthellum indicum are combined, so that the soothing effect is achieved; the embryo extract is subjected to enzymolysis purification to obtain small-molecule active peptide and amino acid, the moisturizing timeliness and transdermal efficiency are remarkably improved, a step-by-step low-temperature mixing process is adopted in the preparation process, the stability of active ingredients is reserved to the maximum extent, and the product overcomes the technical defects that traditional toning lotion is short in moisturizing time and single in effect.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a lotion with rapid moisturizing, firming and soothing effects and a preparation method thereof. Background Art

[0002] Current lotions commonly face three technical bottlenecks: First, they rely on a single moisturizing ingredient, such as glycerin, which holds moisture for a short time and fails to meet long-term hydration requirements; second, firming ingredients are poorly compatible with moisturizing systems, leading to delamination or active loss; and third, high-efficacy products often contain irritating ingredients, such as alcohol, making it difficult to achieve a soothing effect. Although embryo extract is rich in bioactive substances, its application in lotions has yet to achieve breakthroughs, particularly in its synergistic mechanisms with polyols and metal ions.

[0003] Existing technologies attempt to improve efficacy by combining plant extracts or synthetic peptides, but these efforts have failed to establish a synergistic multi-system network. Some products utilize high-temperature emulsification processes, which inactivate heat-sensitive ingredients. Therefore, there is an urgent need to develop a toner formula and supporting low-temperature preparation process that simultaneously achieves rapid moisturizing, long-lasting firming, and gentle soothing effects. Summary of the Invention

[0004] The purpose of this section is to summarize some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the abstract and title of this application to avoid obscuring the purpose of this section, the abstract and the title of the invention, and such simplifications or omissions should not be used to limit the scope of the present invention.

[0005] In view of the above problems in the prior art, the inventors proposed the present invention.

[0006] Therefore, the purpose of the present invention is to overcome the deficiencies in the prior art and provide a lotion with rapid moisturizing, firming and soothing effects and a preparation method thereof.

[0007] To solve the above technical problems, the present invention provides the following technical solution: a lotion with rapid moisturizing, firming and soothing effects, comprising, by mass percentage: 0.095% embryo extract, 6% glycerin, 6% propylene glycol, 1.5% panthenol, 0.2% copper gluconate, 0.1% golden chamomile extract, 0.08% purslane extract, 0.1% hydrolyzed sodium hyaluronate, 0.36% 1,2-hexanediol, 0.06% caprylhydroxamic acid, and the balance being deionized water.

[0008] As a preferred embodiment of the toner with rapid moisturizing, firming and soothing effects of the present invention, the embryo extract is prepared by the following steps:

[0009] a) Sheep embryonic tissue was removed from the viscera, homogenized, and deionized water was added at a solid-to-liquid ratio of 1:10, and the pH was adjusted to 6.8-7.2;

[0010] b) Add 2% papain by weight of the tissue and perform enzymatic hydrolysis at 37°C for 3 h, followed by enzyme inactivation at 70°C for 15 min;

[0011] c) After centrifugation, the product was filtered through a 30,000 Da ultrafiltration membrane and freeze-dried at -50°C for 20 h.

[0012] As a preferred embodiment of the toner with rapid moisturizing, firming and soothing effects described in the present invention, it further comprises 0.01% of Sophora flavescens root extract, 0.6875% of glycerol glucoside and 0.78% of 1,3-propylene glycol.

[0013] As a preferred embodiment of the toner with rapid moisturizing, firming and soothing effects described in the present invention, the pH value of the toner is 5.0-6.0.

[0014] As a preferred embodiment of the toner with rapid moisturizing, firming and soothing effects described in the present invention, its 1-hour skin moisture retention rate is ≥68%, and its 4-hour moisture retention rate is ≥49.6%.

[0015] As a preferred embodiment of the toner with rapid moisturizing, firming and soothing effects of the present invention, the elastase inhibition rate at a concentration of 10% is ≥82.4%.

[0016] As a preferred embodiment of the toner with rapid moisturizing, firming and soothing effects described in the present invention, the hyaluronidase inhibition rate at the stock solution concentration is ≥79.4%.

[0017] A preferred method for preparing a toner with rapid moisturizing, firming and soothing effects comprises:

[0018] a) Preparation of aqueous phase: Dissolve deionized water, glycerol, and propylene glycol at 60-65°C with stirring;

[0019] b) Preparation of the active phase: Dissolve the embryo extract in 10% butylene glycol solution at 40°C, add panthenol and hydrolyzed sodium hyaluronate;

[0020] c) Mixing: Add the active phase to the aqueous phase, stir at 3000 rpm for 8 min, cool to 45°C, add golden chamomile extract and purslane extract, and discharge at 30°C.

[0021] As a preferred solution of the method for preparing the toner with rapid moisturizing, firming and soothing effects of the present invention, the dissolution temperature of the active phase is ≤45°C.

[0022] As a preferred embodiment of the method for preparing a lotion with rapid moisturizing, firming and soothing effects according to the present invention, 1,2-hexanediol and caprylhydroxamic acid are added in step c).

[0023] Beneficial effects of the present invention: The embryo extract leads the synergistic effect of multiple systems: its small molecule peptides and amino acids cooperate with glycerin and propylene glycol to form a three-dimensional moisturizing network, which makes the 1-hour moisturizing rate exceed 68%, and the 4-hour moisturizing rate is still higher than 49.6%; the extract is combined with panthenol and copper ions to significantly enhance the elastase inhibition ability to 82.4%; at the same time, it is combined with plant ingredients such as golden chamomile to achieve a 79.4% hyaluronidase inhibition rate, achieving anti-inflammatory and soothing effects; step-by-step mixing below 40°C avoids denaturation of active peptides, so that the ingredient retention rate exceeds 90% within 6 months; the mild preservative system (1,2-hexanediol + caprylhydroxamic acid) has been confirmed by zebrafish experiments to have an irritation rate of only 3%, breaking through the industry problem of high efficacy and high irritation. This solution is the first to apply embryo extract to lotion and achieve multi-effect synergy. DETAILED DESCRIPTION

[0024] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0025] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0026] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it constitute a separate or selective embodiment that is mutually exclusive with other embodiments.

[0027] Example 1

[0028] This embodiment provides a lotion with rapid moisturizing, firming and soothing effects and a preparation method thereof. The components and mass percentages thereof are as follows:

[0029]

[0030]

[0031] The preparation process of this embodiment adopts a step-by-step low-temperature mixing process, which specifically includes the following steps:

[0032] (1) Preparation of aqueous phase: Deionized water (76.93%), glycerol (6.0%), propylene glycol (6.0%), and 1,3-propylene glycol (0.78%) were added to a reactor and stirred at 300 rpm for 15 min at 62 ± 1°C until they were completely dissolved and formed a homogeneous phase.

[0033] (2) Preparation of active phase: embryo extract (0.095%) and 10% butylene glycol solution (0.95 g) were mixed in a constant temperature water bath at 40 ± 1°C, preferably at a stirring rate of 400 r / min. After dissolution, panthenol (1.5%) and hydrolyzed sodium hyaluronate (0.1%) were added and stirred for 10 min.

[0034] (3) Preparation of functional phase: Golden chamomile extract (0.1%), Portulaca oleracea extract (0.08%), and Sophora flavescens root extract (0.01%) were pre-dispersed with 5% deionized water, and then glycerol glucoside (0.6875%) was added and stirred at 300 r / min for 5 min;

[0035] (4) Mixing: The active phase was slowly added to the aqueous phase, and high-speed shear stirring was performed at 3000 r / min for 8 min; then the temperature was lowered to 45±1°C, and the functional phase, 1,2-hexanediol (0.36%), and octanoylhydroxamic acid (0.06%) were added, and gentle stirring was performed at 200 r / min for 10 min;

[0036] (5) Discharging: Continue to cool to 30±1℃, detect the pH value to be 5.6, filter through 0.22μm microporous membrane and then fill.

[0037] Furthermore, the small molecule peptides (molecular weight <3000Da) in the embryo extract form a hydrogen bond network with glycerol and 1,3-propylene glycol, achieving the three-level effect of "rapid moisture absorption-deep penetration-long-term water lock"; panthenol and the collagen tripeptide in the embryo extract co-activate fibroblasts, and copper gluconate promotes collagen cross-linking by stabilizing the LOXL enzyme domain; bisabolol in golden chamomile and the heat shock protein (HSP70) in the embryo extract jointly inhibit the activity of TRPV1 receptors and block the release pathway of inflammatory factors.

[0038] The effect verification results are as follows:

[0039]

[0040]

[0041] Example 2

[0042] This embodiment provides a lotion with rapid moisturizing, firming and soothing effects and a preparation method thereof.

[0043] Based on Example 1, this example further optimizes the moisturizing system and makes the following changes:

[0044] Increase glycerin to 8.0% and reduce propylene glycol to 4.0%;

[0045] Add 0.08% sodium hyaluronate (molecular weight 80-100kDa) to form a gradient molecular weight permeation network with hydrolyzed sodium hyaluronate;

[0046] The embryo extract concentration was maintained at 0.095%, but the dissolving vehicle was changed to a 5% glycerol-butylene glycol mixture (mass ratio 1:1).

[0047] To accommodate high viscosity components, the following adjustments are made:

[0048] (1) The aqueous phase dissolution temperature is raised to 65±1°C, and the swelling time is extended to 25 minutes to ensure that the sodium hyaluronate is fully hydrated;

[0049] (2) After the active phase is dissolved, let it stand for 5 minutes and then add it dropwise into the aqueous phase to avoid phase separation caused by excessive local concentration;

[0050] (3) An ultrasonic homogenization step (40 kHz, 5 min, power 200 W) was added before discharging.

[0051] Furthermore, the water-locking film was strengthened, and sodium hyaluronate formed a dense hydration film on the epidermis, and the 4-hour moisturizing rate was increased to 51.8% (vs. 49.6% in Example 1);

[0052] Penetration synergy: Small molecule hydrolyzed sodium hyaluronate (molecular weight 5kDa) carries active peptides from embryo extract to penetrate the stratum corneum barrier. As measured by Franz diffusion cell, the transdermal rate increased by 22.7%;

[0053] Stability verification: No delamination after 3 months of accelerated testing at 40°C, pH fluctuation ≤ 0.2 (initial pH = 5.7).

[0054] Example 3

[0055] This embodiment provides a lotion with rapid moisturizing, firming and soothing effects and a preparation method thereof. Targeting sensitive skin, this embodiment focuses on reducing the risk of irritation:

[0056] Strengthened soothing ingredients: golden chamomile extract increased to 0.15%, purslane extract increased to 0.12%;

[0057] Adjustment of the anti-corrosion system: 1,2-hexanediol was reduced to 0.25%, and octanoylhydroxamic acid was reduced to 0.05%;

[0058] New pH buffer: citric acid-sodium citrate 0.05% (maintain pH = 5.8-6.0).

[0059] (1) Plant extract pretreatment: Golden chamomile and purslane extracts were pre-dispersed with 5% glycerol and aged at 40°C for 30 min;

[0060] (2) Low temperature mixing: the temperature of the functional phase is reduced to 40 ± 1 °C and the stirring rate is limited to 150 r / min;

[0061] (3) Light-proof filling: Use amber glass bottles and fill with nitrogen for protection.

[0062] Mildness verification

[0063] Acute toxicity to zebrafish: mortality rate ≤ 3% at 0.25% concentration (P>0.05);

[0064] Human patch test: 30 subjects with sensitive skin showed a 41.2% decrease in redness index after 14 days of use (compared to a 18.7% decrease in the control group).

[0065] Soothing effect: The hyaluronidase inhibition rate was increased to 83.1% (vs 79.4% in Example 1).

[0066] The present invention uses embryo extract as the technical hub to construct a three-dimensional interaction network of "active peptides-polyols-metal ions". The small molecule active peptides (molecular weight <3000Da) and amino acids (accounting for ≥50%) retained in the extract after specific enzymatic purification not only have excellent transdermal ability, but also become a bridge connecting the three major effects of moisturizing, firming and soothing. In the moisturizing dimension, the hydrophilic groups on the peptide chain form a dynamic hydrogen bond network with glycerol and propylene glycol, achieving progressive moisturizing from instantaneous hydration of the stratum corneum to long-term water lock in the granular layer; in the firming dimension, the active peptides activate the fibroblast collagen synthesis pathway together with panthenol by competitively inhibiting the active site of elastase, while trace copper gluconate stabilizes the spatial conformation of the LOXL enzyme. The three form an "inhibition-activation-crosslinking" cascade reaction in the dermis; in the soothing dimension, the heat shock protein (HSP70) in the extract and the bisabolol of golden chamomile synergistically downregulate the expression of TRPV1 receptors and block the conduction of inflammatory signals. This design of using a single ingredient to drive multi-pathway synergy breaks through the limitations of simply stacking functional ingredients in traditional formulas.

[0067] The step-by-step low-temperature mixing process used in the embodiment is essentially a precise regulation of the mass transfer kinetics of active substances. When the embryo extract is dissolved in butanediol at 40°C, its α-helical structure is completely retained, avoiding the unfolding and aggregation of the peptide chains caused by high temperature; and the aqueous phase dissolves the polyol at 62°C, which can fully destroy the hydrogen bond clusters between glycerol molecules and improve its binding efficiency with peptides. The key step of mixing the two phases at 45°C is just above the glass transition temperature (Tg) of the embryo extract and below the denaturation temperature (Td). At this time, the molecular chain segments have moderate mobility, which can not only promote their directional assembly with panthenol and hydrolyzed sodium hyaluronate, but also avoid irreversible aggregation caused by high temperature. The strategy of adding the functional phase last avoids the interaction between plant polyphenols and metal ions (such as Cu 2 The high-temperature chelation reaction ensures the bioavailability of copper gluconate. This process design, based on phase transition behavior, enables an active ingredient retention rate exceeding 90%, far exceeding the industry average of 70%.

[0068] In summary, traditional products focus solely on physical moisturizing of the epidermis or intervention in a single signaling pathway (e.g., peptide anti-wrinkle). However, the small molecule composition (amino acids / peptides / proteins) of embryonic extracts can mimic extracellular matrix signals, activating the dialogue mechanism between keratinocytes and fibroblasts through integrin receptors. Specifically, glycine / proline promotes the expression of the aquaporin AQP3, achieving bidirectional dermal-epidermal water circulation; specific tripeptide sequences (e.g., GHK) act as copper ion carriers, precisely delivering copper ions to the active center of the SOD enzyme; and the rich laminin fragments in the extract can repair the skin barrier. This systematic design, from microscopic cellular communication to macroscopic efficacy expression, addresses the industry's pain points of short moisturizing duration and fragmented efficacy.

[0069] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A lotion with rapid moisturizing, firming and soothing effects, characterized by: The composition comprises, by mass percentage, 0.095% embryo extract, 6% glycerol, 6% propylene glycol, 1.5% panthenol, 0.2% copper gluconate, 0.1% golden chamomile extract, 0.08% purslane extract, 0.1% hydrolyzed sodium hyaluronate, 0.36% 1,2-hexanediol, 0.06% caprylhydroxamic acid, and the balance is deionized water.

2. The lotion with rapid moisturizing, firming and soothing effects according to claim 1, characterized in that: The embryo extract is prepared by the following steps: a) Sheep embryonic tissue was removed from the viscera, homogenized, and deionized water was added at a solid-to-liquid ratio of 1:10, and the pH was adjusted to 6.8-7.2; b) Add 2% papain by weight of the tissue and perform enzymatic hydrolysis at 37°C for 3 h, followed by enzyme inactivation at 70°C for 15 min; c) After centrifugation, the product was filtered through a 30,000 Da ultrafiltration membrane and freeze-dried at -50°C for 20 h.

3. The lotion with rapid moisturizing, firming and soothing effects according to claim 1, characterized in that: It also contains 0.01% of Sophora flavescens root extract, 0.6875% of glyceryl glucoside, and 0.78% of 1,3-propylene glycol.

4. The lotion with rapid moisturizing, firming and soothing effects according to claim 1, characterized in that: The pH value of the lotion is 5.0-6.

0.

5. The lotion with rapid moisturizing, firming and soothing effects according to claim 1, characterized in that: Its 1-hour skin moisture retention rate is ≥68%, and 4-hour moisture retention rate is ≥49.6%.

6. The lotion with rapid moisturizing, firming and soothing effects according to claim 1, characterized in that: The elastase inhibition rate at a concentration of 10% was ≥82.4%.

7. The lotion with rapid moisturizing, firming and soothing effects according to claim 1, characterized in that: The hyaluronidase inhibition rate at the stock solution concentration is ≥79.4%.

8. A method for preparing a lotion with rapid moisturizing, firming and soothing effects according to any one of claims 1 to 7, characterized in that: include: a) Preparation of aqueous phase: Dissolve deionized water, glycerol, and propylene glycol at 60-65°C with stirring; b) Preparation of the active phase: Dissolve the embryo extract in 10% butylene glycol solution at 40°C, add panthenol and hydrolyzed sodium hyaluronate; c) Mixing: Add the active phase to the aqueous phase, stir at 3000 rpm for 8 min, cool to 45°C, add golden chamomile extract and purslane extract, and discharge at 30°C.

9. The method for preparing a lotion with rapid moisturizing, firming and soothing effects according to claim 8, characterized in that: The active phase dissolution temperature is ≤45°C.

10. The method for preparing a lotion with rapid moisturizing, firming and soothing effects according to claim 8, characterized in that: In step c), 1,2-hexanediol and octanoylhydroxamic acid are added.

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