Natural plant composite hoof bath lotion and preparation method thereof

Abstract

The invention relates to the field of veterinary cow hoof care, in particular to natural plant composite hoof bath lotion and a preparation method thereof. The natural plant composite hoof bath lotion comprises a coptis chinensis extract, a yucca extract, an astragalus membranaceus extract, a surfactant and a thickener. Compared with the prior art, the technical scheme of the invention has the following beneficial effects: (1) the natural plant composite hoof bath lotion does not contain alcohols, aldehydes, antibiotics, copper sulfate and other components, so that threats to biological food safety and pollution to the environment are avoided; and (2) the natural plant composite hoof bath lotion not only can effectively kill and inhibit pathogenic microorganisms at lesions of the hoof of the dairy cow, but also is rich in various natural plant components, and can promote hemostasis recovery of wounds of the hoof. (3) the components of the natural plant composite hoof bath lotion disclosed by the invention have synergism: coptis chinensis (antibacterial), bletilla striata (repairing) and astragalus mongholicus (immunological enhancement) are synergic and are obviously superior to single or double components.

Description

Technical Field

[0001] The present application relates to the field of veterinary dairy cow hoof care, and in particular to a natural plant composite hoof bath and a preparation method thereof. Background Art

[0002] Hoof diseases are common in ruminant farming, with incidence rates exceeding 30% in some areas, significantly impacting farm profitability. Summer is the peak season for hoof diseases. There are numerous types of hoof diseases, including sole ulcers, toe ulcers, white line disease, dermatitis, laminitis, and foot rot. Hoof diseases are often caused by infection with various pathogens, including Treponema, Fusobacterium, Porphyromonas, and Bacteroides. Hoof diseases are caused by a variety of factors, including genetic defects in the animal breed itself; excessively damp and dirty barns with hard floors; an imbalanced calcium-phosphorus ratio in the feed, deficiencies in trace elements such as zinc and selenium; and the lack of regular hoof trimming or excessive hoof trimming. Hoof diseases often result from a combination of genetic, environmental, nutritional, and management factors.

[0003] Regardless of the specific hoof disease, ruminants often experience lameness, decreased feed intake, reduced production performance, reproductive failure, and high culling rates, resulting in significant economic losses for farms. Common treatments for hoof disease on cattle farms include hoof trimming and hoof baths. In the past, hoof baths often contained high levels of alcohol and aldehydes, which often resulted in significant environmental pollution and posed a significant risk to animal food safety.

[0004] The prior art CN115300538A uses chemical antibacterial agents such as thymol and glutaraldehyde to compound with Chinese herbal extracts, but the synergistic mechanism between the plant components is not clear.

[0005] The existing technology patent CN115869353A mainly contains glutaraldehyde and quaternary ammonium salts, supplemented by traditional Chinese medicine ingredients, and has poor adaptability to complex environments.

[0006] However, as consumers' food safety standards continue to rise, traditional hoof bath products are increasingly unable to meet consumer demand.

[0007] Therefore, there is an urgent need to develop an environmentally friendly hoof bath based on the synergistic effect of multiple plants. Summary of the Invention

[0008] The purpose of the present application is to provide a natural plant compound hoof bath solution, comprising coptis root extract, yucca extract, astragalus extract, a surfactant and a thickener.

[0009] In certain embodiments, the Coptidis Rhizoma extract is extracted by reflux extraction with 70% ethanol.

[0010] In certain embodiments, the yucca extract is prepared by alkaline extraction and acid precipitation.

[0011] In certain embodiments, the Astragalus extract is prepared by water extraction and alcohol precipitation.

[0012] In certain embodiments, by weight, the ingredients include 5-10 parts of Coptis chinensis extract, 5-10 parts of Yucca schidigera extract, and 5-10 parts of Astragalus membranaceus extract.

[0013] In certain embodiments, by weight, 8 parts of Coptis chinensis extract, 8 parts of Yucca schidigera extract, and 8 parts of Astragalus membranaceus extract are used.

[0014] In certain embodiments, the surfactant is sodium lauryl sulfate and the thickener is sodium carboxymethyl cellulose.

[0015] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0016] (1) The natural plant compound hoof bath solution of the present application does not contain ingredients such as alcohols, aldehydes, antibiotics, and copper sulfate, thereby avoiding threats to biological food safety and pollution to the environment.

[0017] (2) The natural plant compound hoof bath liquid of the present application can not only effectively kill and inhibit pathogenic microorganisms in lesions on the hooves of dairy cows, but is also rich in a variety of natural plant ingredients, which can promote hemostasis and recovery of hoof wounds.

[0018] (3) Synergy of the components of the natural plant composite hoof bath solution of the present application: Coptis chinensis (antibacterial), Bletilla striata (repair), and Astragalus membranaceus (immune enhancement) work synergistically, which is significantly better than single or double components. DETAILED DESCRIPTION

[0019] The exemplary embodiments of the present disclosure will be described in more detail below. It should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. Instead, these embodiments are provided to enable a more thorough understanding of the present disclosure and to fully convey the scope of the present disclosure to those skilled in the art.

[0020] Example 1: Preparation of plant extracts

[0021] 1. Coptis chinensis extract:

[0022] Take 100 g of Coptis chinensis powder, extract it with 70% ethanol at a ratio of 1:8 under reflux twice (1 h / time), combine the filtrates, and concentrate them to an extract, which is the Coptis chinensis extract.

[0023] 2. Yucca Extract:

[0024] 100 g of Bletilla striata tuber slices were cold-soaked in 0.1% NaOH solution for 48 hours, filtered, and then 1 M HCl was added to adjust the pH to 4. The precipitate was precipitated and dried to obtain the yucca extract.

[0025] 3. Astragalus Extract:

[0026] 100 g of Astragalus slices were extracted with water (60°C, 2 h) and concentrated, and ethanol was added to 60% for precipitation. The ethanol was recovered to obtain the Astragalus extract.

[0027] Example 2: Preparation of composite hoof bath solution

[0028] Formula: 8g of Coptis chinensis extract, 8g of Yucca schidigera extract, 8g of Astragalus membranaceus extract, 1.2g of sodium lauryl sulfate, 0.3g of sodium carboxymethyl cellulose, and deionized water to make up to 100g.

[0029] Procedure: Mix the extract and excipients, homogenize (20 MPa, 5 min), and sterilize at 85°C for 30 min.

[0030] Example 3: Preparation of composite hoof bath solution

[0031] Formula: 10g of Coptis chinensis extract, 6g of Yucca schidigera extract, 8g of Astragalus membranaceus extract, and the rest are the same as in Example 2.

[0032] Example 4: Preparation of composite hoof bath solution

[0033] Formula: 6g of Coptis chinensis extract, 8g of Yucca schidigera extract, 10g of Astragalus membranaceus extract, and the rest are the same as in Example 2.

[0034] Example 5: Preparation of composite hoof bath solution

[0035] Formula: 8g of Coptis chinensis extract, 10g of Yucca schidigera extract, 6g of Astragalus membranaceus extract, and the rest are the same as in Example 2.

[0036] Comparative Example 1

[0037] Formula: 12 g of Yucca schidigera extract, 12 g of Astragalus membranaceus extract, omitting the Coptis chinensis extract, and the rest is the same as in Example 2.

[0038] Comparative Example 2

[0039] Formula: 12 g of coptis root extract, 12 g of astragalus root extract, omitting the yucca extract, and the rest is the same as in Example 2.

[0040] Comparative Example 3

[0041] Formula: 12 g of coptis root extract, 12 g of yucca extract, omitting the astragalus root extract, and the rest is the same as in Example 2.

[0042] Comparative Example 4

[0043] Formula: 24 g of Astragalus extract, omitting Coptis chinensis extract and Yucca schidigera extract, and the rest is the same as in Example 2.

[0044] Comparative Example 5

[0045] Formula: 24 g of Coptis chinensis extract, omitting Astragalus membranaceus extract and Yucca schidigera extract, and the rest is the same as in Example 2.

[0046] Comparative Example 6

[0047] Formula: 24 g of Yucca schidigera extract, omitting the Coptis chinensis extract and Astragalus membranaceus extract, and the rest is the same as in Example 2.

[0048] Example 6, Antibacterial Rate Test

[0049] Detailed steps:

[0050] Experimental design

[0051] 1. Strains and culture medium:

[0052] Strains: Salmonella (ATCC 14028), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 6538), Fusobacterium nucleatum (ATCC 25586).

[0053] Culture medium: Nutrient agar (NA) is used for bacterial culture, and MH agar (Mueller-Hinton Agar) is used for drug sensitivity testing.

[0054] 2. Sample preparation:

[0055] The hoof bath stock solutions of Examples 2-5 and Comparative Examples 1-6 were diluted with sterile physiological saline to a concentration of 10% (simulating the actual usage concentration).

[0056] 3. Inhibition zone determination (agar diffusion method):

[0057] Inoculation: Use an inoculation loop to take the bacterial solution in the logarithmic growth phase and evenly spread it on the surface of the NA plate (about 1×10 6 CFU).

[0058] Sample addition: Place a sterile Oxford cup on the surface of the bacterial layer and inject 200 μL of different sample dilutions respectively.

[0059] Culture: Culture at 37°C for 18-24 hours.

[0060] Measurement: The diameter of the inhibition zone (mm) was measured using a vernier caliper, with each group repeated three times.

[0061] 4. Data Analysis:

[0062] Inhibition rate (%) = (average diameter of the inhibition zone of the experimental group - diameter of the inhibition zone of the control group) / diameter of the inhibition zone of the control group × 100%.

[0063] SPSS 26.0 was used to perform one-way analysis of variance (ANOVA) and Dunnett's T3 test for multiple comparisons, with a significance level of α=0.05.

[0064] 5. Experimental verification:

[0065] Positive control: Vancomycin (inhibition zone ≥ 25 mm) was used to verify the reliability of the method.

[0066] Negative control: sterile saline (inhibition zone ≤ 8 mm).

[0067] The results are shown in Table 1.

[0068] Table 1 Antibacterial results

[0069]

[0070] The results show that the compound hoof bath solution of Example 2 has the highest inhibition rate against the four pathogens.

[0071] Example 7: Hoof disease prevention effect

[0072] Normal and healthy Holstein cows were selected and divided into control group 1 (clear water), control group 2 (commercially available glutaraldehyde hoof bath), and experimental groups (Example 2 and Comparative Examples 1-6). Hoof baths were performed on 20 cows in each group, repeated three times. Usage: Thoroughly clean the cow's hoof to ensure that there is no feces residue in the hoof and toe space. Dilute the hoof bath 20-50 times with water and then add it to the cow's hoof bath. The liquid level is at least 15 cm. The cow's hoof is soaked in the hoof bath for 20 minutes to complete a hoof bath.

[0073] Frequency: Once every 3 days for 30 consecutive days.

[0074] Environmental control: The temperature of the experimental cattle house was 18-22℃, the humidity was ≤60%, and the manure was cleaned every day.

[0075] Wound treatment: If there is a minor abrasion, disinfect with iodine and continue the test. It will not be included in the incidence statistics.

[0076] Observation indicators:

[0077] Morbidity statistics:

[0078] Hoof rot: hoof sole ulcer area ≥ 2cm 2 or positive exudate;

[0079] Verrucous dermatitis: interdigital skin hyperplasia ≥1 cm or scab;

[0080] Hoof ulcer: Deep tissue damage visible to the naked eye.

[0081] Data recording: Two veterinarians independently evaluated the data every week using a double-blind method.

[0082] Incidence rate (%) = (number of infected cattle / total number of cattle) × 100%;

[0083] The chi-square test was used to compare the differences between the groups, and P < 0.05 was considered significant.

[0084] The results are shown in Tables 2 to 4.

[0085] Table 2. Foot rot prevention results

[0086]

[0087] Table 3. Results of prevention of wart dermatitis

[0088]

[0089]

[0090] Table 4. Results of prevention of hoof sole ulcers

[0091]

[0092] *Indicates P < 0.05 compared with the commercially available group.

[0093] The results showed that the incidence of hoof disease in the water group was significantly higher than in the experimental group, indicating that simple rinsing is ineffective in preventing and controlling hoof disease. The incidence of commercially available glutaraldehyde was significantly lower than that in the water group, but higher than that in Example 2, demonstrating that the formulation of the present invention is superior to traditional chemical preparations. Furthermore, the results in Tables 2-4 show that Example 2 had a highly significant effect compared to the water group (P < 0.01) and a significant effect compared to the commercially available group (P < 0.05), suggesting that the plant components in Example 2 synergistically enhance protective effects.

[0094] The foregoing description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any modifications or substitutions that can be readily conceived by a person skilled in the art within the technical scope disclosed herein are intended to be encompassed within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be subject to the scope of protection of the claims.

Claims

1. A natural plant compound hoof bath, characterized in that: Contains Coptis chinensis extract, Yucca schidigera extract, Astragalus membranaceus extract, as well as surfactants and thickeners.

2. The hoof bath liquid according to claim 1, characterized in that The Coptidis rhizome extract is extracted by reflux extraction with 70% ethanol.

3. The hoof bath liquid according to claim 1, characterized in that Yucca schidigera extract was prepared by alkaline extraction and acid precipitation.

4. The hoof bath according to claim 1, wherein Astragalus extract was prepared by water extraction and alcohol precipitation method.

5. The hoof bath according to claim 1, wherein By weight, the ingredients include 5-10 parts of coptis root extract, 5-10 parts of yucca extract, and 5-10 parts of astragalus root extract.

6. The hoof bath according to claim 1, characterized in that By weight, 8 parts of coptis root extract, 8 parts of yucca extract, and 8 parts of astragalus extract.

7. The hoof bath according to any one of claims 1 to 6, characterized in that The surfactant is sodium lauryl sulfate and the thickener is sodium carboxymethyl cellulose.

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