Cat food capable of conditioning spleen and stomach of cat and preparation method of cat food

Abstract

The invention discloses cat food for conditioning spleen and stomach of cats and a preparation method of the cat food. The cat food comprises a cat food base material, modified tapioca flour, modified silicon dioxide, MCT oil, beta-sitosterol and sodium hydrogen sulfate. The modified silicon dioxide loaded gamma-cyclodextrin preferentially adsorbs moisture, accelerates hydrolysis of sodium hydrogen sulfate and releases H < + > in a high-humidity environment, an imine bond between the gamma-cyclodextrin and the glycerol monocaprate is hydrolyzed and broken under acidity, the MCT oil reduces the critical micelle concentration of the glycerol monocaprate and a stearic acid alkyl chain, hydrophobic micro-area fusion is promoted, and the hydrophobic property of the hydrophobic micro-area is improved. The beta-sitosterol anchors alkyl chains of the decanoic acid monoglyceride and the stearic acid at the same time, shortens the distance and improves Van der Waals' force, so that the decanoic acid monoglyceride and the stearic acid are crosslinked through hydrophobic association and the Van der Waals' force to generate contractility, the nanocellulose crystal is pulled to contract, the microporous structure of the cat food is pulled to contract and close, and then water invasion is prevented. And the damp-proof purpose is achieved.

Description

Technical Field

[0001] The invention belongs to the field of pet feed, in particular to cat food for regulating the spleen and stomach of cats and a preparation method thereof. Background Art

[0002] Baked cat food, due to its slow, low-temperature baking process, retains higher levels of protein and vitamins than puffed cat food. However, it also tends to be harder. For healthy adult cats, hard pellets can reduce plaque and prevent tartar through friction. However, for kittens, chewing can be difficult or even painful. Furthermore, kittens have delicate stomachs, making unchewed pellets difficult to digest and potentially causing vomiting and loose stools.

[0003] The high porosity of porous cassava flour can significantly reduce the hardness of baked cat food. However, because cassava flour contains a large number of hydrophilic hydroxyl groups, it actively absorbs moisture from the air, causing the particles to soften and swell. Furthermore, the high porosity increases the moisture absorption surface, making the cat food more susceptible to moisture and spoilage. Deteriorating cat food that absorbs moisture not only loses nutrients but also breeds mold, especially Aspergillus flavus, which is extremely harmful to cats' livers and can cause acute liver damage or even death. Summary of the Invention

[0004] (1) Technical problems to be solved

[0005] The present invention aims to provide a cat food for regulating the spleen and stomach of cats and a preparation method thereof, so as to solve the problem that the cat food is easily affected by moisture due to porous cassava flour.

[0006] (2) Technical solution

[0007] To achieve the above object, on the one hand, the present invention provides a cat food for regulating the spleen and stomach of a cat, comprising the following components in parts by weight: 60-100 parts of cat food base, 10-15 parts of modified cassava flour, 3-8 parts of modified silicon dioxide, 1-3 parts of MCT oil, 0.1-0.3 parts of β-sitosterol, and 0.1-0.2 parts of sodium bisulfate;

[0008] The modified cassava flour is a porous material with nanocellulose crystals with stearic acid grafted on the surface embedded in the pores;

[0009] The modified silica supports aldehyde-modified γ-cyclodextrin and amino-modified capric monoglyceride.

[0010] Furthermore, the preparation method of the modified cassava flour comprises the following steps:

[0011] S11. The nanocellulose crystals and deionized water were homogenized under high pressure to obtain a uniform suspension, epichlorohydrin was added, NaOH was added dropwise to adjust the pH, and the suspension was purified by dialysis with deionized water to obtain a first compound;

[0012] S12. Stearic acid and anhydrous ethanol were dissolved by heating in a water bath, and the first compound was added. The mixture was ultrasonically dispersed, and EDC·HCl and NHS were added sequentially. The pH was adjusted with dilute HCl. The reaction was stirred magnetically, and acetate buffer was added. The precipitate was collected by centrifugation, washed sequentially with ethanol, NaHCO3 solution, and ultrapure water, and freeze-dried to obtain the second compound.

[0013] S13. Add cassava flour and glycerol to hot water, stir to gelatinize, add trisodium citrate and trehalose and continue stirring, the slurry is injected into the mold, heated in an oven, frozen with liquid nitrogen, and vacuum dried to obtain porous cassava flour;

[0014] S14. The second compound is dispersed in anhydrous ethanol. The porous cassava flour is spread on a mesh basket and immersed in the dispersion of the second compound. Vacuuming is performed to maintain pressure, and the pressure is slowly released. The mixture is allowed to stand, and the vacuuming is repeated three times. The mixture is freeze-dried to obtain modified cassava flour.

[0015] Furthermore, the porosity of the porous cassava flour is 55-65%.

[0016] Furthermore, the added amount of the nanocellulose crystals is less than 2% of the dry weight of the cat food.

[0017] Furthermore, the preparation method of the modified silicon dioxide comprises the following steps:

[0018] S21. Dissolve γ-cyclodextrin in deionized water, add NaIO4 under dark conditions, stir the reaction, oxidize the hydroxyl group to form an aldehyde group, add ethylene glycol, stir, dialysis, and freeze-dry to obtain a first compound;

[0019] S22. Decanoic acid monoglyceride and 3-amino-1,2-propanediol were added to toluene, p-toluenesulfonic acid was added and refluxed, washed with NaHCO3 solution, dried over anhydrous sodium sulfate, and the toluene was removed by rotary evaporation to obtain a second compound;

[0020] S23. Mix the first compound and the second compound, dissolve in anhydrous methanol, add activating molecular sieves, react with stirring under nitrogen protection, remove the molecular sieves by filtration, concentrate the filtrate by rotary evaporation, wash with ethanol, and dry in vacuo to obtain the third compound;

[0021] S24. The mesoporous silica was dispersed in anhydrous toluene, APTES was added, and the reaction was refluxed under nitrogen. The solid was collected by centrifugation, washed with ethanol, and dried in vacuo to obtain a fourth compound;

[0022] S25. The third compound is dissolved in anhydrous ethanol, glacial acetic acid is added, and the fourth compound is added. The mixture is dispersed by ultrasonication and shaken for reaction. The solid is collected by centrifugation, washed with methanol, and dried in vacuo to obtain modified silica.

[0023] Furthermore, the cat food base includes chicken, chicken heart, chicken liver powder, fish meal, fruit and vegetable powder, fish oil and Chinese medicine powder; the Chinese medicine powder includes malt, coix seed, tangerine peel and hawthorn.

[0024] On the other hand, based on the same inventive concept, the present invention also provides a method for preparing cat food for regulating cat spleen and stomach, which is applied to the cat food for regulating cat spleen and stomach, comprising the following steps:

[0025] S1. Raw material preparation: Mince chicken, chicken heart, and chicken liver, add protease for enzymatic hydrolysis, add fish meal, fruit and vegetable powder, fish oil, and traditional Chinese medicine powder, and mix well to obtain a cat food base; ultrasonically disperse modified silica in ethanol, add β-sitosterol, and shake the mixture. The reaction mixture is centrifuged, washed with cold ethanol, and vacuum dried to obtain a first mixture;

[0026] S2. Mixing and granulation: After the cat food base and modified cassava flour are mixed evenly, they are put into a pellet forming machine and extruded into pellets;

[0027] S3 Baking: The particles are placed in a baking device for baking, the baking temperature is 60 to 80 ° C, the moisture content of the dried particles is less than 8%;

[0028] S4. Vacuum spraying: The first mixture and sodium bisulfate are homogeneously dispersed in the MCT oil, and then sprayed onto the surface of the dried particles under high pressure to obtain cat food.

[0029] Cassava flour foams and creates pores, which increases the porosity. High porosity can significantly reduce the hardness of baked cat food, making it more suitable for kittens. However, due to the increased porosity, the microporous structure of cat food is more likely to absorb moisture from the air. Therefore, nanocellulose crystals (CNCs) with stearic acid grafted on the surface are pre-embedded in the pores of the modified cassava flour to work synergistically with modified silica. Modified silica is loaded with aldehyde-modified γ-cyclodextrin (γ-CD) and amino-modified monoglyceride of capric acid (GMC). The aldehyde group of γ-CD forms an imine bond with the amino group of GMC. In a high-humidity environment, since the outer surface of the γ-CD cavity is hydrophilic, it pre-absorbs moisture, forming a local high-humidity environment, accelerating the hydrolysis of sodium bisulfate, and releasing H +, causing a local pH drop, triggering the hydrolysis and cleavage of the imine bond between γ-CD and GMC in acidic conditions, intelligently releasing GMC. GMC then crosslinks with the stearic acid grafted onto the surface of CNCs through hydrophobic association and van der Waals forces, generating a contractile force. The CNCs contract under tension, pulling the microporous structure of the cat food closed and preventing water intrusion. The hydroxyl groups on the surface of the modified silica form a hydrogen bond network with the CNCs, preventing particle breakage due to concentrated contraction stress. Furthermore, the released GMC acts as an antibacterial agent, inhibiting mold growth. When humidity decreases, the hydration layer on the γ-cyclodextrin surface disappears, the pH in the microregion returns to neutral, and the free γ-CD-CHO re-condenses with the GMC-NH2. The hydrophobic association weakens, the CNCs stretch, and the pores of the tapioca starch reopen, restoring the cat food's crispness.

[0030] Because monoglyceride caprate has a short alkyl chain and lacks conjugation with stearic acid, their hydrophobic association is weak. Therefore, the addition of β-sitosterol, a natural product, not only reduces cholesterol absorption and prevents toxic accumulation, but also allows its hydrophobic core to anchor the alkyl chains of both monoglyceride caprate and stearic acid, shortening the distance and enhancing van der Waals interactions. MCT oil can lower the critical micelle concentration of monoglyceride caprate and the alkyl chains of stearic acid, promoting the fusion of hydrophobic microdomains. Furthermore, because β-sitosterol has a slightly bitter taste and may cause cats to refuse to eat, encapsulating the β-sitosterol core with a γ-cyclodextrin cavity blocks its binding to bitter taste receptors on the tongue. In high humidity, γ-cyclodextrin preferentially absorbs water, increasing the hydrophilicity of the cavity and promoting the release of β-sitosterol. β-sitosterol, through its hydrophobic bridging function, works with MCT oil to promote hydrophobic association and van der Waals crosslinking between monoglyceride caprate and stearic acid.

[0031] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0032] 1. The present invention replaces the adhesive cassava flour in cat food with porous cassava flour, which significantly reduces the hardness of the cat food and is more suitable for kittens to eat.

[0033] 2. Porous cassava flour increases the porosity of cat food, making it easier for moisture to invade, causing the cat food to become damp and deteriorate. The present invention pre-embeds nanocellulose crystals with surface grafted stearic acid in the pores of the porous cassava flour and synergizes with monoglyceride of capric acid loaded on modified silica. Under high humidity, stearic acid and monoglyceride of capric acid generate contraction force through hydrophobic association and van der Waals force cross-linking. The nanocellulose crystals shrink under tension, pulling the microporous structure of the cat food to shrink and close, thereby preventing moisture intrusion.

[0034] 3. The present invention further strengthens the hydrophobic association and van der Waals cross-linking of capric monoglyceride and stearic acid by adding β-sitosterol and MCT oil. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 The present invention is a flow chart for preparing cat food for regulating the spleen and stomach of cats;

[0036] Figure 2 This is a photo of the cat food prepared in Example 1 of the present invention;

[0037] Figure 3 This is a physical picture of the cat food prepared in Comparative Example 1 of the present invention. DETAILED DESCRIPTION

[0038] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0039] Example 1

[0040] This embodiment discloses a cat food for regulating the spleen and stomach of a cat, comprising the following components in parts by weight: 60 parts of a cat food base, 10 parts of modified cassava flour, 5 parts of modified silicon dioxide, 1 part of MCT oil, 0.1 part of β-sitosterol, and 0.1 part of sodium bisulfate.

[0041] The modified cassava flour is a porous material with nanocellulose crystals with stearic acid grafted on the surface embedded in the pores;

[0042] The modified silica supports aldehyde-modified γ-cyclodextrin and amino-modified capric monoglyceride.

[0043] The preparation method of the modified cassava flour comprises the following steps:

[0044] S11. The nanocellulose crystals and deionized water were homogenized under high pressure to obtain a uniform suspension, epichlorohydrin was added, NaOH was added dropwise to adjust the pH, and the suspension was purified by dialysis with deionized water to obtain a first compound;

[0045] S12. Dissolve stearic acid and anhydrous ethanol in a water bath, add the first compound, and disperse by ultrasonication. Then, add EDC·HCl and NHS sequentially, adjust the pH with dilute HCl, and react with magnetic stirring. Add acetate buffer, collect the precipitate by centrifugation, wash sequentially with ethanol and ultrapure water, and dry in vacuo to obtain the second compound.

[0046] S13. Add cassava flour and glycerol to hot water, stir to gelatinize, add trisodium citrate and trehalose and continue stirring, the slurry is injected into the mold, heated in an oven, frozen with liquid nitrogen, and vacuum dried to obtain porous cassava flour;

[0047] S14. The second compound is dispersed in anhydrous ethanol. The porous cassava flour is spread on a mesh basket and immersed in the dispersion of the second compound. Vacuuming is performed to maintain pressure, and the pressure is slowly released. The mixture is allowed to stand, and the vacuuming is repeated three times. The mixture is freeze-dried to obtain modified cassava flour.

[0048] The porosity of the porous tapioca flour is 50%.

[0049] The added amount of the nanocellulose crystals is 1% of the dry weight of the cat food.

[0050] It should be noted that if the porosity of cassava flour is less than 50%, foaming is insufficient, resulting in limited improvement in hardness and unable to meet the needs of kittens. If the porosity of cassava flour is higher than 65%, the specific surface area increases dramatically, leading to excessively high water adsorption rates, which may exceed the response speed limit of nanocellulose crystal contraction and closure, resulting in localized moisture-proof failure. Excessive porosity can also significantly weaken the mechanical strength of the matrix and increase the risk of breakage during transportation.

[0051] The preparation method of the modified silicon dioxide comprises the following steps:

[0052] S21. Dissolve γ-cyclodextrin in deionized water, add NaIO4 under dark conditions, stir the reaction, oxidize the hydroxyl group to form an aldehyde group, add ethylene glycol, stir, dialysis, and freeze-dry to obtain a first compound;

[0053] S22. Decanoic acid monoglyceride and 3-amino-1,2-propanediol were added to toluene, p-toluenesulfonic acid was added and refluxed, washed with NaHCO3 solution, dried over anhydrous sodium sulfate, and the toluene was removed by rotary evaporation to obtain a second compound;

[0054] S23. The first compound and the second compound were mixed in a mass ratio of 5:3, dissolved in anhydrous methanol, and activated molecular sieves, react with stirring under nitrogen protection, remove the molecular sieves by filtration, concentrate the filtrate by rotary evaporation, wash with ethanol, and dry in vacuo to obtain the third compound;

[0055] S24. The mesoporous silica was dispersed in anhydrous toluene, APTES was added, and the reaction was refluxed under nitrogen. The solid was collected by centrifugation, washed with ethanol, and dried in vacuo to obtain a fourth compound;

[0056] S25. The third compound is dissolved in anhydrous ethanol, glacial acetic acid is added, and the fourth compound is added. The mixture is dispersed by ultrasonication and shaken for reaction. The solid is collected by centrifugation, washed with methanol, and dried in vacuo to obtain modified silica.

[0057] The cat food base includes chicken, chicken heart, chicken liver, fish meal, fruit and vegetable powder, fish oil and traditional Chinese medicine powder; the traditional Chinese medicine powder includes malt, coix seed, tangerine peel and hawthorn.

[0058] The method for preparing cat food for regulating the spleen and stomach of cats comprises the following steps:

[0059] S1. Raw material preparation: Mince chicken, chicken heart, and chicken liver, add protease for enzymatic hydrolysis, add fish meal, fruit and vegetable powder, fish oil, and traditional Chinese medicine powder, and mix well to obtain a cat food base; ultrasonically disperse modified silica in ethanol, add β-sitosterol, and shake the mixture. The reaction mixture is centrifuged, washed with cold ethanol, and vacuum dried to obtain a first mixture;

[0060] S2. Mixing and granulation: After the cat food base and modified cassava flour are mixed evenly, they are put into a pellet forming machine and extruded into pellets;

[0061] S3 Baking: The particles are placed in a baking device for baking, the baking temperature is 60 to 80 ° C, the moisture content of the dried particles is less than 8%;

[0062] S4. Vacuum spraying: The first mixture and sodium bisulfate are homogeneously dispersed in the MCT oil, and then sprayed onto the surface of the dried particles under high pressure to obtain cat food.

[0063] It should be noted that if Figure 1 Shown is a flow chart for preparing cat food for regulating the spleen and stomach of cats in this embodiment. In the raw material preparation stage, since β-sitosterol is easily oxidized at high temperature to produce a bitter taste that causes cats to refuse to eat, the cavity of modified silica-loaded γ-cyclodextrin is used to wrap β-sitosterol. In a high humidity environment, γ-cyclodextrin preferentially adsorbs moisture, the hydrophilicity of the cavity increases, and β-sitosterol is released. Sodium bisulfate is easily decomposed at high temperature, so after mixing granulation and low-temperature baking, the first mixture and sodium bisulfate are homogeneously dispersed on the surface of the cat food by vacuum spraying of MCT oil, and the material temperature needs to be controlled to be less than 40°C during spraying.

[0064] Example 2

[0065] This example is based on Example 1, but differs from Example 1 in that it includes the following components by weight: 80 parts cat food base, 12 parts modified cassava flour, 6 parts modified silicon dioxide, 2 parts MCT oil, 0.2 parts β-sitosterol, and 0.15 parts sodium bisulfate. Other components and preparation methods are the same as in Example 1.

[0066] Example 3

[0067] This example is based on Example 1, but differs from Example 1 in that it includes the following components by weight: 100 parts cat food base, 15 parts modified cassava flour, 8 parts modified silicon dioxide, 3 parts MCT oil, 0.3 parts β-sitosterol, and 0.2 parts sodium bisulfate. Other components and preparation methods are the same as in Example 1.

[0068] Example 4

[0069] This embodiment is based on Example 1, but differs from Example 1 in that the porosity of the porous cassava flour in this embodiment is 65%. Other components and preparation methods are the same as those in Example 1.

[0070] Example 5

[0071] This example is based on Example 1, but differs from Example 1 in that the amount of nanocellulose crystals added in this example is 2% of the dry weight of the cat food. Other components and preparation methods are the same as in Example 1.

[0072] Comparative Example 1

[0073] This example is based on Example 1, but different from Example 1 in that the porosity of the porous cassava flour in this comparative example is 70%. If the porosity is too high, the mechanical strength of the matrix will be significantly reduced, and the prepared cat food will be Figure 2 As shown, the brittleness is too great, causing the cat food to break into pieces and lose its shape.

[0074] Other components and preparation methods are the same as those in Example 1.

[0075] Comparative Example 2

[0076] This example is based on Example 1, but differs from Example 1 in that the amount of nanocellulose crystals added in this comparative example is 0% of the dry weight of the cat food.

[0077] The preparation method of the modified cassava flour comprises the following steps:

[0078] S11. Add cassava flour and glycerol to hot water, stir and gelatinize, add trisodium citrate and trehalose and continue stirring, inject the slurry into the mold, heat in an oven, freeze with liquid nitrogen, and vacuum dry to obtain modified cassava flour;

[0079] Other components and preparation methods are the same as those in Example 1.

[0080] Comparative Example 3

[0081] This example is based on Example 1, but differs from Example 1 in that stearic acid is not grafted onto the surface of the nanocellulose crystals during the preparation of the modified cassava flour in this comparative example.

[0082] The preparation method of the modified cassava flour comprises the following steps:

[0083] S11. The nanocellulose crystals and deionized water were homogenized under high pressure to obtain a uniform suspension, epichlorohydrin was added, and NaOH was added dropwise to adjust the pH. The reaction solution was dialyzed with deionized water to obtain a first compound;

[0084] S12. Add cassava flour and glycerol to hot water, stir to gelatinize, add trisodium citrate and trehalose and continue stirring, inject the slurry into the mold, heat in an oven, freeze the keyhole with liquid nitrogen, and vacuum dry to obtain porous cassava flour;

[0085] S13. The first compound is dispersed in anhydrous ethanol, the porous cassava flour is spread on a mesh basket, and the porous cassava flour is immersed in the dispersion of the first compound. Vacuuming and maintaining the pressure, slowly releasing the pressure, standing, and repeating the vacuuming three times. The modified cassava flour is freeze-dried to obtain the modified cassava flour.

[0086] Other components and preparation methods are the same as those in Example 1.

[0087] Comparative Example 4

[0088] This example is based on Example 1, but differs from Example 1 in that the γ-cyclodextrin in this comparative example is not treated with NaIO4, and the capric monoglyceride is not treated with 3-amino-1,2-propylene glycol. That is, the γ-cyclodextrin and capric monoglyceride do not form an imine bond, but instead react to form an ester bond. Because the ester bond is irreversible, the moisture-proofing effect is lost after several dry-wet cycles.

[0089] The preparation method of the modified silicon dioxide comprises the following steps:

[0090] S21. γ-cyclodextrin, monoglyceride and p-toluenesulfonic acid were mixed, anhydrous xylene and molecular sieves were added, nitrogen was bubbled to deoxygenate, the reaction was refluxed, the reaction solution was filtered, the precipitate was washed with hot ethanol, and vacuum dried to obtain a first compound;

[0091] S22. The mesoporous silica was dispersed in anhydrous toluene, APTES was added, and the reaction was refluxed under nitrogen. The solid was collected by centrifugation, washed with ethanol, and dried in vacuo to obtain a second compound;

[0092] S23. The first compound is dissolved in anhydrous ethanol, glacial acetic acid is added, and the second compound is added. The mixture is dispersed by ultrasonication and shaken for reaction. The solid is collected by centrifugation, washed with methanol, and dried in vacuo to obtain modified silica.

[0093] Other components and preparation methods are the same as those in Example 1.

[0094] Comparative Example 5

[0095] This example is based on Example 1, but is different from Example 1 in that the modified silica in this comparative example does not load γ-cyclodextrin.

[0096] The preparation method of the modified silicon dioxide comprises the following steps:

[0097] S21. Decanoic acid monoglyceride and 3-amino-1,2-propylene glycol were added to toluene, p-toluenesulfonic acid was added and refluxed, washed with NaHCO3 solution, dried over anhydrous sodium sulfate, and the toluene was removed by rotary evaporation to obtain a first compound;

[0098] S22. The mesoporous silica was dispersed in anhydrous toluene, APTES was added, and the reaction was refluxed under nitrogen. The solid was collected by centrifugation, washed with ethanol, and dried in vacuo to obtain a second compound;

[0099] S23. The first compound is dissolved in anhydrous ethanol, glacial acetic acid is added, and the second compound is added. The mixture is dispersed by ultrasonication and shaken for reaction. The solid is collected by centrifugation, washed with methanol, and dried in vacuo to obtain modified silica.

[0100] Other components and preparation methods are the same as those in Example 1.

[0101] Comparative Example 6

[0102] This example is based on Example 1, but differs from Example 1 in that the modified silica in this comparative example does not carry monoglyceride of caprate.

[0103] The preparation method of the modified silicon dioxide comprises the following steps:

[0104] S21. Dissolve γ-cyclodextrin in deionized water, add NaIO4 under dark conditions, stir the reaction, oxidize the hydroxyl group to form an aldehyde group, add ethylene glycol, stir, dialysis, and freeze-dry to obtain a first compound;

[0105] S22. The mesoporous silica was dispersed in anhydrous toluene, APTES was added, and the reaction was refluxed under nitrogen. The solid was collected by centrifugation, washed with ethanol, and dried in vacuo to obtain a second compound;

[0106] S23. The first compound is dissolved in anhydrous ethanol, glacial acetic acid is added, and the third compound is added. The mixture is dispersed by ultrasonication and shaken for reaction. The solid is collected by centrifugation, washed with methanol, and dried in vacuo to obtain modified silica.

[0107] Other components and preparation methods are the same as those in Example 1.

[0108] Comparative Example 7

[0109] This example is based on Example 1, but differs from Example 1 in that no modified cassava flour is added. Other components and preparation methods are the same as those in Example 1.

[0110] Comparative Example 8

[0111] This example is based on Example 1, but differs from Example 1 in that modified silicon dioxide is not added. Other components and preparation methods are the same as those in Example 1.

[0112] Comparative Example 9

[0113] This example is based on Example 1, but differs from Example 1 in that sodium bisulfate is not added. Other components and preparation methods are the same as those in Example 1.

[0114] Comparative Example 10

[0115] This example is based on Example 1, but differs from Example 1 in that β-sitosterol is not added. Other components and preparation methods are the same as those in Example 1.

[0116] Comparative Example 11

[0117] This example is based on Example 1, but differs from Example 1 in that MCT oil is not added. Other components and preparation methods are the same as those in Example 1.

[0118] Comparative Example 12

[0119] This example is based on Example 1, but differs from Example 1 in that this comparative example is a blank control group, which includes the following components in parts by weight: 60 parts of cat food base and 10 parts of cassava flour.

[0120] The method for preparing cat food for regulating the spleen and stomach of cats comprises the following steps:

[0121] S1. Prepare the ingredients: Mince the chicken, chicken heart, and chicken liver, add protease for enzymatic hydrolysis, add fish meal, fruit and vegetable powder, fish oil, and Chinese herbal medicine powder, and mix well to obtain the cat food base.

[0122] S2. Mixing and granulation: Put the cat food base and tapioca flour into the pellet forming machine and extrude the pellets;

[0123] S3 Baking: The particles are placed in a baking device for baking, the baking temperature is 60 to 80 ° C, the moisture content of the dried particles is less than 8%;

[0124] Test verification:

[0125] 1. Moisture resistance: The prepared cat food samples were placed in a storage environment at 30°C and 75% RH for 90 days. The changes in the quality of the cat food were recorded and the moisture absorption rate was calculated.

[0126] 2. Antibacterial properties: Inoculate cat food samples with common molds, place them in an environment of 28°C and 75% RH for 7 days, and calculate the antibacterial rate.

[0127] 3. Mechanical Properties: Cat food samples were stored at 40% RH for 6 hours, and the breaking force was measured using a texture analyzer, recorded as F1. The same cat food samples were stored at 75% RH for 6 hours, and the breaking force was measured using a texture analyzer, recorded as F2. The humidity was restored to 40% RH and the cat food was stored for 6 hours, and the breaking force was measured using a texture analyzer, recorded as F3. After 10 days of this cycle, the pore closure rate and brittleness recovery rate of the cat food were calculated.

[0128] Calculate pore closure efficiency: η 1 =(F 2 ―F 1 ) / F 1 ×100%.

[0129] Calculate the brittle recovery rate: η2=|F 3 ―F 1 | / F 1 ×100%.

[0130] 4. Molding effect: Observe whether the cat food sample is formed and whether the particles are complete and full.

[0131] Table 1. Cat food performance test table

[0132]

[0133] As can be seen from Table 1, modified cassava flour, modified silicon dioxide, MCT oil, β-sitosterol, and sodium bisulfate work synergistically in moisture-proofing cat food. Modified silicon dioxide-loaded γ-cyclodextrin preferentially absorbs moisture in a high-humidity environment, accelerates the hydrolysis of sodium bisulfate, and releases H + , the imine bond between γ-cyclodextrin and monoglyceride of capric acid is hydrolyzed and broken under acidic conditions, MCT oil reduces the critical micelle concentration of monoglyceride of capric acid and the alkyl chain of stearic acid, promotes the fusion of hydrophobic micro-regions, and β-sitosterol anchors the alkyl chains of monoglyceride of capric acid and stearic acid at the same time, shortens the distance, and enhances the van der Waals force, so that monoglyceride of capric acid and stearic acid are cross-linked through hydrophobic association and van der Waals force, generating a shrinkage force, and CNCs shrink under tension, pulling the microporous structure of the cat food to shrink and close, thereby preventing water intrusion and achieving the purpose of moisture-proofing. It can be seen from comparative examples 5, 6, and 8 that the released monoglyceride of capric acid has a strong inhibitory effect on mold. After multiple dry-wet cycles, it can be seen from the data of Example 1 and the comparative example that the cat food sample prepared by the present invention still has good pore closure and brittle recovery. As shown Figure 2 The cat food sample of Example 1 of the present invention is shown in the figure. The forming effect is good, the particles are complete and there is no breakage. However, Figure 3 The figure shows a real cat food sample prepared in comparative example 1 of the present invention. The molding effect is poor and the particles are broken. This is mainly because the porosity of cassava flour is too high, which leads to a significant reduction in the mechanical strength of the matrix and excessive brittleness, resulting in the cat food being broken and not forming.

[0134] The above description is only a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions and improvements made by any technician familiar with the field within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A cat food for regulating cat spleen and stomach, characterized in that, The invention comprises the following components in parts by weight: 60-100 parts of cat food base, 10-15 parts of modified cassava flour, 3-8 parts of modified silicon dioxide, 1-3 parts of MCT oil, 0.1-0.3 parts of β-sitosterol, and 0.1-0.2 parts of sodium bisulfate; The modified cassava flour is a porous material with nanocellulose crystals with stearic acid grafted on the surface embedded in the pores; The modified silica supports aldehyde-modified γ-cyclodextrin and amino-modified capric monoglyceride.

2. A cat food for regulating cat spleen and stomach according to claim 1, characterized in that, The preparation method of the modified cassava flour comprises the following steps: S11. The nanocellulose crystals and deionized water were homogenized under high pressure to obtain a uniform suspension, epichlorohydrin was added, NaOH was added dropwise to adjust the pH, and the suspension was purified by dialysis with deionized water to obtain a first compound; S12. Stearic acid and anhydrous ethanol were dissolved by heating in a water bath, and the first compound was added. The mixture was ultrasonically dispersed, and EDC·HCl and NHS were added sequentially. The pH was adjusted with dilute HCl. The reaction was stirred magnetically, and acetate buffer was added. The precipitate was collected by centrifugation, washed sequentially with ethanol, NaHCO3 solution, and ultrapure water, and freeze-dried to obtain the second compound. S13. Add cassava flour and glycerol to hot water, stir to gelatinize, add trisodium citrate and trehalose and continue stirring, the slurry is injected into the mold, heated in an oven, frozen with liquid nitrogen, and vacuum dried to obtain porous cassava flour; S14. The second compound is dispersed in anhydrous ethanol. The porous cassava flour is spread on a mesh basket and immersed in the dispersion of the second compound. Vacuuming is performed to maintain pressure, and the pressure is slowly released. The mixture is allowed to stand, and the vacuuming is repeated three times. The mixture is freeze-dried to obtain modified cassava flour.

3. A cat food for regulating cat spleen and stomach according to claim 2, characterized in that, The porosity of the porous cassava flour is 55-65%.

4. A cat food for regulating cat spleen and stomach according to claim 2, characterized in that, The added amount of the nanocellulose crystals is less than 2% of the dry weight of the cat food.

5. A cat food for regulating cat spleen and stomach according to claim 1, characterized in that, The preparation method of the modified silicon dioxide comprises the following steps: S21. Dissolve γ-cyclodextrin in deionized water, add NaIO4 under dark conditions, stir the reaction, oxidize the hydroxyl group to form an aldehyde group, add ethylene glycol, stir, dialysis, and freeze-dry to obtain a first compound; S22. Decanoic acid monoglyceride and 3-amino-1,2-propanediol were added to toluene, p-toluenesulfonic acid was added and refluxed, washed with NaHCO3 solution, dried over anhydrous sodium sulfate, and the toluene was removed by rotary evaporation to obtain a second compound; S23. Mix the first compound and the second compound, dissolve in anhydrous methanol, add activating molecular sieves, react with stirring under nitrogen protection, remove the molecular sieves by filtration, concentrate the filtrate by rotary evaporation, wash with ether, and dry in vacuo to obtain the third compound; S24. The mesoporous silica was dispersed in anhydrous toluene, APTES was added, and the reaction was refluxed under nitrogen. The solid was collected by centrifugation, washed with ethanol, and dried in vacuo to obtain a fourth compound; S25. The third compound is dissolved in anhydrous ethanol, glacial acetic acid is added, and the fourth compound is added. The mixture is dispersed by ultrasonication and shaken for reaction. The solid is collected by centrifugation, washed with methanol solution, and dried in vacuo to obtain modified silica.

6. A cat food for regulating cat spleen and stomach according to claim 1, characterized in that, The cat food base includes chicken, chicken heart, chicken liver, fish meal, fruit and vegetable powder, fish oil and traditional Chinese medicine powder; the traditional Chinese medicine powder includes malt, coix seed, tangerine peel and hawthorn.

7. A method for preparing cat food for regulating cat spleen and stomach, which is used to prepare the cat food for regulating cat spleen and stomach as claimed in any one of claims 1 to 5, characterized in that: The method comprises the following steps: S1. Raw material preparation: Mince chicken, chicken heart, and chicken liver, add protease for enzymatic hydrolysis, add fish meal, fruit and vegetable powder, fish oil, and traditional Chinese medicine powder, and mix well to obtain a cat food base; ultrasonically disperse modified silica in ethanol, add β-sitosterol, and shake the mixture. The reaction mixture is centrifuged, washed with cold ethanol, and vacuum dried to obtain a first mixture; S2. Mixing and granulation: After the cat food base and modified cassava flour are mixed evenly, they are put into a pellet forming machine and extruded into pellets; S3 Baking: The particles are placed in a baking device for baking, the baking temperature is 60 to 80 ° C, the moisture content of the dried particles is less than 8%; S4. Vacuum spraying: The first mixture and sodium bisulfate are homogeneously dispersed in the MCT oil, and then sprayed onto the surface of the dried particles under high pressure to obtain cat food.

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