Processing method of ganoderma sinense spore extract suspension with anti-tumor effect

Abstract

The invention belongs to the technical field of polysaccharide medicine extraction, and discloses a processing method of a ganoderma sinense spore extract suspension with an anti-tumor effect, which comprises the following steps: adding a ganoderma sinense spore extract solid into a mixed solution of ganoderma sinense spore oil and glyceryl monostearate, and uniformly mixing; the weight ratio of solid to liquid is 1: (8-10), and the mass of the glycerin monostearate is 1-1.5% of the mass of the ganoderma sinense spore oil. According to the invention, fat-soluble components and water-soluble components in the ganoderma sinense spore powder are respectively extracted, mixed and prepared into the ganoderma sinense spore powder full-component health-care food, the full components of the ganoderma sinense spore powder full-component health-care food are fully utilized, and the tumor inhibition rate of the ganoderma sinense spore extract suspension is obviously superior to that of a ganoderma lucidum spore powder extract suspension and ganoderma sinense spore oil.

Description

Technical Field

[0001] The invention belongs to the technical field of polysaccharide drug extraction, and particularly relates to a method for processing a suspension of a purple ganoderma spore extract having anti-tumor effects. Background Art

[0002] Ganoderma lucidum spore powder is a kind of Ganoderma lucidum from the family Polyporaceae. Ganoderma lucidum (Leyss. ex Fr.) Karst.), Ganoderma lucidum ( Ganoderma sinense Zhao, Xu et Zhang), Ganoderma lucidum ( Ganoderma tsugae ) The main production areas are in Jiangxi, Zhejiang, Fujian, Liaoning, Jilin, and Guizhou provinces in my country. Research on the chemical composition of Ganoderma lucidum spore powder by scholars from various countries has revealed a rich and diverse chemical composition, primarily including polysaccharides, triterpenes, sterols, terpenoids, amino acids, and alkaloids. Among these, polysaccharides, triterpenes, and sterols are found in relatively high concentrations and are also the primary components that exert their pharmacological effects. Pharmacological activity studies have reported that the main components of Ganoderma lucidum exhibit antioxidant, anti-tumor, hypoglycemic, hypolipidemic, anti-inflammatory, anti-aging, immunomodulatory, hepatoprotective, and neuroprotective effects.

[0003] In recent years, many scholars at home and abroad have conducted in-depth research on the active ingredients and pharmacological effects of Ganoderma lucidum spore powder. Health foods using Ganoderma lucidum spore powder as a primary ingredient, such as cracked Ganoderma lucidum spore powder and Ganoderma lucidum spore oil, have excellent health benefits and market value. However, while Ganoderma lucidum spore powder is readily available and inexpensive, research reports and utilization of Ganoderma lucidum spore powder are relatively limited, and its active ingredients are not well understood. Summary of the Invention

[0004] The invention deeply processes the purple ganoderma spore powder, extracts fat-soluble components and water-soluble components therein respectively, and mixes and prepares the extracts to prepare a health food containing all the components of the purple ganoderma spore powder.

[0005] The object of the present invention is achieved in the following ways: A method for processing a suspension of a spore extract of ganoderma lucidum with anti-tumor effect comprises the following steps: adding a solid of the spore extract of ganoderma lucidum to a mixture of spore oil of ganoderma lucidum and glyceryl monostearate and mixing them evenly, wherein the weight ratio of the solid to the liquid is 1:8-10, and the mass of the glyceryl monostearate is 1-1.5% of the mass of the spore oil of ganoderma lucidum.

[0006] Preferably, the solid-liquid weight ratio is 1:9.

[0007] Preferably, the mass of the glyceryl monostearate is 1% of the mass of the Ganoderma lucidum spore oil.

[0008] The processing method of the above-mentioned purple Ganoderma lucidum spore extract suspension with anti-tumor effect specifically comprises the following steps: 1) Preparation of Ganoderma lucidum spore oil: breaking the spores of Ganoderma lucidum to a breaking rate of over 90%, granulating, and extracting to obtain Ganoderma lucidum spore oil and Ganoderma lucidum spore granules; 2) Preparation of Ganoderma lucidum spore extract: Soak the Ganoderma lucidum spore particles after degreasing in step 1) in 100°C water for 10-12 hours, decoct with water 2-3 times, filter each extract separately, combine, concentrate, collect into a paste, and vacuum dry to obtain a Ganoderma lucidum spore extract dry paste, which is then crushed to obtain Ganoderma lucidum spore extract; 3) Preparation of Ganoderma lucidum spore extract suspension: Ganoderma lucidum spore oil and glyceryl monostearate are stirred and mixed at 65-75°C, with the mass of glyceryl monostearate being 1-1.5% of the mass of Ganoderma lucidum spore oil. After the glyceryl monostearate is completely dissolved, Ganoderma lucidum spore extract is added at a solid-to-liquid weight ratio of 1:8-10. The suspension is stirred at 65-75°C until the solution is uniform, and then ground and mixed uniformly using a colloid mill to obtain the suspension.

[0009] Preferably, the cell wall breaking in step 1) is specifically to add the spore powder of Ganoderma lucidum into Bailey mill for cell wall breaking for 30-50 minutes, so as to achieve a cell wall breaking rate of more than 90%.

[0010] Preferably, after the cell wall breaking in step 1) is completed, granulation is performed using a granulator, and the granules are evenly spread on a drying tray for drying, with a thickness of 2-4 cm, a drying temperature of 100-120° C., and a drying time of 1-2 hours.

[0011] Preferably, the extraction in step 1) is to put the granulated Ganoderma lucidum spore powder particles into a CO2 supercritical extraction device, set the extraction pressure to 25-35 MPa, the temperature to 40-50°C, and extract for 3-4 hours to obtain purple Ganoderma lucidum spore oil and Ganoderma lucidum spore particles.

[0012] Preferably, the water decoction in step 2) is performed twice, the first time with 8-10 times the weight of the decoction being decocted for 2-3 hours, and the second time with 6-8 times the weight of the decoction being decocted for 1-2 hours.

[0013] Preferably, the colloid mill in step 3) is used for uniform mixing for 3-8 minutes.

[0014] Preferably, in step 3), the magnetic stirring is continued at 65-75° C. for 30-40 minutes until the solution becomes a homogeneous oil liquid.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. Both purple Ganoderma lucidum spore oil and purple Ganoderma lucidum spore extract have anti-tumor effects, and the tumor inhibition effect is better when the two are mixed and used together. However, the two raw materials are liquid and solid respectively, and direct mixing will result in stratification. The present invention uses glyceryl monostearate as a suspending agent for the suspension in this formula to achieve better dispersion promotion and anti-sedimentation capabilities, and the tumor inhibition rate of the suspension is higher than that of using it alone, with an effect of 1+1>2. At the same time, the tumor inhibition rate of the purple Ganoderma lucidum spore extract suspension is significantly better than that of the red Ganoderma lucidum spore extract suspension, and the cost is low.

[0016] 2. The present invention extracts fat-soluble components and water-soluble components from the spore powder of Ganoderma lucidum respectively, and the products prepared by mixing and preparing the components can effectively make full use of all the components of the spore powder of Ganoderma lucidum. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Picture 1 The figure shows a comparison of the appearance and particle size under a microscope of the suspensions obtained using glyceryl monostearate and Span 80 as surfactants in Example 1 and Comparative Examples 1-3.

[0018] Picture 2 The figure shows the comparison of the appearance and particle size under the microscope of suspensions with different monostearate contents. DETAILED DESCRIPTION

[0019] The following is a detailed description of the specific embodiments of the present invention. It should be understood that the specific embodiments described herein are only used to illustrate and explain the present invention and are not intended to limit the present invention.

[0020] In the specific embodiments of the present invention, unless otherwise specified, the reagents and materials used are conventional commercial products in the field and can be obtained through conventional commercial channels. The purple Ganoderma lucidum spore powder used in the examples is the dried mature spores of the Polyporaceae fungus Ganoderma lucidum (Leyss. ex Fr.) Karst., purchased from Anhui Huangshan Yunle Ganoderma Co., Ltd., with a brown uniform powder appearance, no mildew, no lumps, no impurities, and a unique smell of Ganoderma lucidum spores. The polysaccharide content is ≥1.0g / 100g, the moisture content is ≤10g / 100g, the ash content is ≤3.0g / 100g, the lead (as Pb) content is ≤1.5mg / kg, the total arsenic (as As) content is ≤1.0mg / kg, and the mercury (as Hg) content is ≤0.3mg / kg. Example 1

[0021] Preparation of Purple Ganoderma Lucidum Spore Oil: Purple Ganoderma Lucidum spore powder is placed in a Bailey mill for 30 minutes to a 95% spore breaking rate. After spore breaking, the spores are granulated using a pelletizer. The spores are evenly spread onto a baking tray to an appropriate thickness of 2-4 cm and dried at 110°C for 1.5 hours. After granulation, the spores are placed in a supercritical CO2 extraction apparatus at a pressure of 30 MPa and a temperature of approximately 45°C for approximately 4 hours to obtain Purple Ganoderma Lucidum spore oil and degreased spore granules.

[0022] Preparation of purple Ganoderma lucidum spore extract: After degreasing, the Ganoderma lucidum spore particles are soaked in an extraction tank at 100°C for 12 hours, and decocted with water twice, the first time with 10 times the weight of water for 3 hours, and the second time with 8 times the weight of water for 2 hours. The two extracts are filtered through a plate and frame filter respectively, temporarily stored in an intermediate storage tank, and concentrated to an appropriate density under reduced pressure in a double-effect concentrator to collect the paste. The dry paste of Ganoderma lucidum spore extract is obtained by vacuum drying, and then the fine powder extract of Ganoderma lucidum spore extract is obtained by crushing.

[0023] Preparation of Ganoderma lucidum spore oil and spore extract suspension: set the water temperature of the mixing tank to 70°C, add Ganoderma lucidum spore oil and glyceryl monostearate into the mixing tank and stir, the mass of glyceryl monostearate is 1% of the mass of Ganoderma lucidum spore oil, wait until the glyceryl monostearate is completely dissolved, add Ganoderma lucidum spore powder extract in a solid-liquid weight ratio of 1:9, continue magnetic stirring for 30 minutes to fully mix until the solution is uniform, open the bottom discharge valve after mixing, put the oil into the colloid mill bin, mix it again evenly for 5 minutes after passing through the colloid mill, and put the mixture into the oil storage tank.

[0024] After testing: the polysaccharide content is 2.5g / 100g, the ergosterol content is 150.3 mg / 100g, and the Ganoderma triterpenoid content is 20.2 g / 100g. Example 2

[0025] Preparation of Ganoderma lucidum spore oil: Purple Ganoderma lucidum spore powder is added to a Bailey mill for 30 minutes to a 95% spore breaking rate. After spore breaking, the spores are granulated using a granulator. The spores are evenly spread on a drying tray to an appropriate thickness of 2-4 cm and dried at 100°C for 2 hours. After granulation, the spores are placed in a supercritical CO2 extraction apparatus at an extraction pressure of 30 MPa and a temperature of approximately 45°C for approximately 4 hours to obtain purple Ganoderma lucidum spore oil and degreased Ganoderma lucidum spore granules.

[0026] Preparation of purple Ganoderma lucidum spore extract: After degreasing, the Ganoderma lucidum spore particles are soaked in an extraction tank at 100°C for 12 hours, and decocted with water twice, the first time with 10 times the weight of water for 3 hours, and the second time with 8 times the weight of water for 2 hours. The two extracts are filtered through a plate and frame filter respectively, temporarily stored in an intermediate storage tank, and concentrated to an appropriate density under reduced pressure in a double-effect concentrator to collect the paste. The dry paste of Ganoderma lucidum spore extract is obtained by vacuum drying, and then the fine powder extract of Ganoderma lucidum spore extract is obtained by crushing.

[0027] Preparation of Ganoderma lucidum spore oil and spore extract suspension: set the water temperature of the mixing tank to 70°C, add Ganoderma lucidum spore oil and glyceryl monostearate into the mixing tank and stir, the mass of glyceryl monostearate is 1% of the mass of Ganoderma lucidum spore oil, wait until the glyceryl monostearate is completely dissolved, add Ganoderma lucidum spore powder extract in a solid-liquid weight ratio of 1:8, continue magnetic stirring for 25 minutes until the solution is uniform, after mixing, open the bottom discharge valve, put the oil into the colloid mill bin, mix it again evenly through the colloid mill for 5 minutes, and put the mixture into the oil storage tank. Example 3

[0028] Preparation of Ganoderma lucidum spore oil: Purple Ganoderma lucidum spore powder is added to a Bailey mill for 30 minutes to a 90% or higher spore breaking rate. After spore breaking, the spores are granulated using a pelletizer. The spores are evenly spread onto a baking tray to an appropriate thickness of 2-4 cm and dried at 120°C for one hour. After granulation, the spores are then placed in a supercritical CO2 extraction apparatus at a pressure of 30 MPa and a temperature of approximately 45°C for approximately four hours to obtain purple Ganoderma lucidum spore oil and degreased Ganoderma lucidum spore granules.

[0029] Preparation of purple Ganoderma lucidum spore extract: After degreasing, the Ganoderma lucidum spore particles are soaked in an extraction tank at 100°C for about 12 hours, and decocted with water twice, the first time with 10 times the weight of water for 3 hours, and the second time with 8 times the weight of water for 2 hours. The two extracts are filtered through a plate and frame filter respectively, temporarily stored in an intermediate storage tank, and concentrated to an appropriate density under reduced pressure in a double-effect concentrator to collect the paste. The dry paste of Ganoderma lucidum spore extract is obtained by vacuum drying, and then crushed to obtain a fine powder extract of Ganoderma lucidum spore extract.

[0030] Preparation of Ganoderma lucidum spore oil and spore extract suspension: set the water temperature of the mixing tank to 70°C, add Ganoderma lucidum spore oil and glyceryl monostearate into the mixing tank and stir, the mass of glyceryl monostearate is 1% of the mass of Ganoderma lucidum spore oil, wait until the glyceryl monostearate is completely dissolved, add Ganoderma lucidum spore powder extract in a solid-liquid weight ratio of 1:10, continue magnetic stirring for 30 minutes until the solution is uniform, after mixing, open the bottom discharge valve, put the oil into the colloid mill bin, mix it again evenly for 5 minutes after passing through the colloid mill, and put the mixture into the oil storage tank.

[0031] Comparative Example 1 The remaining steps were the same as those in Example 1, except that the mixture after adding the Ganoderma lucidum spore powder extract in a solid-liquid weight ratio of 1:9 was subjected to high-speed shearing for 5 minutes (4000 rpm) and cooled in an ice water bath during shearing.

[0032] Comparative Example 2 The remaining steps are the same as those in Example 1, except that glyceryl monostearate is replaced with a corresponding dose of Span 80.

[0033] Comparative Example 3 The remaining steps were the same as those in Example 1, except that glyceryl monostearate was replaced with a corresponding dose of Span 80, and the mixture after adding the Ganoderma lucidum spore powder extract at a solid-liquid ratio of 1:9 was subjected to high-speed shearing for 5 min (4000 rpm), and the mixture was cooled in an ice water bath during shearing.

[0034] A small amount of samples were taken from Example 1 and Comparative Examples 1-3, and the changes in the powder particle size were observed under a microscope after different periods of time. The properties of the samples were also observed after 24 hours of storage. Picture 1 .

[0035] Microscopic images show that Span 80 provides superior dispersion results compared to glyceryl monostearate, whether dispersed using magnetic stirring or high-speed shearing. Comparing the microscopic images of magnetic stirring and high-speed shearing, the dispersion effect of high-speed shearing was inferior to that of magnetic stirring, and even showed some particle aggregation. However, after 24 hours of aging, the Span 80 group showed significant sedimentation, while the particles in the glyceryl monostearate group remained relatively uniform, indicating that glyceryl monostearate provided a superior suspending effect.

[0036] Experimental Example 1: Effect of the amount of surfactant on the dispersion of solid powder The maximum daily dose of glyceryl monostearate for oral administration was 272 mg. The weight of glyceryl monostearate was set to 0.5%, 1%, 1.5%, and 2% of the amount of purple Ganoderma lucidum spore oil, respectively. After heating and melting, it was added to the purple Ganoderma lucidum spore oil (prepared according to the method of Example 1) and then to the purple Ganoderma lucidum spore powder extract (prepared according to the method of Example 1) to obtain 4 samples. The samples were magnetically stirred for 0.5 h, and a small amount of sample was taken and observed under a microscope for changes in powder particle size. The properties of the samples were observed 24 h later. The specific sample amounts are shown in Table 1.

[0037]

[0038] Appearance and particle size inspection, the results are shown in Picture 2 2) Investigation of sedimentation ratio and redispersibility After the magnetic stirring, the suspension of Ganoderma lucidum spore extract was poured into a graduated cylinder, and the initial volume H0 was recorded. The sedimentation volume (H0) was recorded at different time points, such as 3 h and until no change. 3h 、H ∝ ), calculate the sedimentation volume ratio F (F 3h 、F ∝ ), the results are shown in Table 2.

[0039]

[0040] In summary, the above four groups of samples all formed a relatively uniform brown suspension with no obvious powder particles at the bottom; as the amount of monostearate increased, the particle size distribution became more uniform and the powder particles were more evenly distributed; when the monostearate reached 1% of the mass of the purple ganoderma spore oil, a good anti-sedimentation ability could be achieved, and it gradually increased with the increase in dosage, but the enhancement effect was not obvious. At the same time, as the amount of monostearate increased, the redispersibility weakened. The last group had abnormal data, which may be because the viscosity of this group was too high and the original conditions could not make it completely precipitate, resulting in a low redispersion time. In summary, the most preferred amount of monostearate is 1% of the mass of the purple ganoderma spore oil.

[0041] Test Example 2 1. Experimental Purpose The purpose of testing drug samples 1-4 was to determine whether they had growth inhibitory effects on mouse sarcoma S180 cell transplants and the intensity of the effects, providing experimental basis for clinical application.

[0042] 2. Experimental Materials 2.1 Test Drugs 2.1.1 Sample No. 1 Name: Sample No. 1, Ganoderma lucidum spore oil (prepared according to the method of Example 1) Appearance: Yellow oily solution Concentration: 800mg / mL Preparation method: Measure an appropriate amount of sample No. 1 working solution, and then dilute it with soybean oil (manufacturer: Shandong Ruisheng Pharmaceutical Excipients Co., Ltd., batch number: DD20210701, specification: 500 mL) to 50 mg / mL, 25 mg / mL, and 12.5 mg / mL working solutions, respectively. The administration volume is 0.4 ml / 20 g, that is, the administration doses are 1000 mg / kg, 500 mg / kg, and 250 mg / kg, respectively.

[0043] 2.1.2 Sample No. 2 Name: Sample No. 2, Ganoderma lucidum spore extract (prepared according to the method of Example 1) Appearance: brown powder Preparation Method: Accurately weigh 0.10g of Sample No. 2 and add 10ml of 0.5% CMC-Na to prepare a 10.0 mg / mL solution. Accurately weigh 0.05g of Sample No. 2 and add 10ml of 0.5% CMC-Na to prepare a 5.0 mg / mL solution. Accurately weigh 0.025g of Sample No. 2 and add 10ml of 0.5% CMC-Na to prepare a 2.5 mg / mL solution. The resulting solutions have concentrations of 10.0 g / mL, 5.0 g / mL, and 2.5 g / mL. The dosing volume is 0.4 mL / 20g, resulting in doses of 200 mg / kg, 100 mg / kg, and 50 mg / kg.

[0044] 2.1.3 Sample No. 3 Name: Sample No. 3 Properties: Ganoderma lucidum spore oil spore extract suspension (prepared according to the method of Example 1) Concentration: 800mg / mL Preparation method: Measure an appropriate amount of sample No. 3 working solution, and then dilute it with soybean oil (manufacturer: Shandong Ruisheng Pharmaceutical Excipients Co., Ltd., batch number: DD20210701, specification: 500 mL) to 50 mg / mL, 25 mg / mL, and 12.5 mg / mL working solutions, respectively. The administration volume is 0.4 ml / 20 g, that is, the administration doses are 1000 mg / kg, 500 mg / kg, and 250 mg / kg, respectively.

[0045] 2.1.4 Sample No. 4 Name: Sample No. 4 Properties: Ganoderma lucidum spore oil spore extract suspension (preparation method is the same as Example 1, the difference is that the raw material is Ganoderma lucidum) Concentration: 800mg / mL Preparation method: Measure an appropriate amount of sample No. 4 working solution, and then dilute it with soybean oil (manufacturer: Shandong Ruisheng Pharmaceutical Excipients Co., Ltd., batch number: DD20210701, specification: 500 mL) to 50 mg / mL, 25 mg / mL, and 12.5 mg / mL working solutions, respectively. The administration volume is 0.4 ml / 20 g, that is, the administration doses are 1000 mg / kg, 500 mg / kg, and 250 mg / kg, respectively.

[0046] 2.1 Comparator drugs Name: 5-FU (5-fluorouracil) Provided by: Tianjin Jinyao Pharmaceutical Co., Ltd. Preparation method: The specification of one bottle of injection is 0.25g / 10ml. Take 1ml and add 7.33ml of normal saline (manufacturer: Jiangsu Huaian Shuanghe Pharmaceutical Co., Ltd.; specification: 500ml: 4.5g) and shake well to obtain 3mg / ml solution. Store at 4℃. The dosage volume is 0.2ml / 20g, that is, the dosage is 30mg / kg.

[0047] 2.2 Experimental animals Source, strain, and species: ICR mice were provided by Hangzhou Medical College (production) (Experimental Animal Production License: SCXK (Zhejiang) 2019-0002).

[0048] Age: 6-8 weeks Gender: Female 2.3 Experimental animal groups are shown in Table 3

[0049] 2.4 Experimental methods ICR mice were inoculated with ascites tumors according to the transplant tumor research method. Ascites fluid was obtained under sterile conditions and diluted with saline to a 1:4 cell suspension. The container was placed on ice and aspirated with a blank needle. The cells were mixed before each aspiration. Each mouse was inoculated subcutaneously with 0.2 ml of the suspension in the right forelimb axilla. Twenty-four hours after inoculation, the mice were weighed and randomly divided into 14 groups. Each group received its first dose 24 hours after inoculation (d1). Samples 1-4 were administered orally once daily for a total of nine doses. The dose volume was 0.4 ml / 20 g. Tumor-bearing mice were sacrificed and weighed on day 10 (d10) after inoculation. The tumor masses were isolated and weighed, and the data were statistically analyzed (t-test).

[0050] 3. Results and Discussion The experimental results of the mouse sarcoma S180 cell transplantation tumor model are shown in Table 4: Sample No. 1 was administered orally at doses of 1000 mg / kg, 500 mg / kg, and 250 mg / kg, once a day for 10 days; Sample No. 2 was administered orally at doses of 200 mg / kg, 100 mg / kg, and 50 mg / kg, once a day for 10 days; Sample No. 3 was administered orally at doses of 1000 mg / kg, 500 mg / kg, and 250 mg / kg, once a day for 10 days; Sample No. 4 was administered orally at doses of 1000 mg / kg, 500 mg / kg, and 250 mg / kg, once a day for 10 days; The positive drug 5-FU was injected into the tail vein at 30 mg / kg, once every 2 days, with a dosing cycle of 10 days.

[0051] During the experiment, no significant effect of the dose groups of samples 1-4 on the body weight of the experimental animals was found, but the positive drug 5-FU had a very significant inhibitory effect on the body weight of the experimental animals.

[0052]

[0053] 4. Experimental Conclusion Both purple Ganoderma lucidum spore oil and purple Ganoderma lucidum spore extract have anti-tumor effects, but the tumor inhibition rate of the suspension of the two is higher than when used alone, showing a 1+1>2 effect. The tumor inhibition rate of the purple Ganoderma lucidum spore extract suspension is also significantly higher than that of the red Ganoderma lucidum spore powder extract suspension.

Claims

1. A method for processing a suspension of a spore extract of Ganoderma lucidum having an anti-tumor effect, characterized in that The method comprises the following steps: adding purple ganoderma lucidum spore extract solid into a mixture of purple ganoderma lucidum spore oil and glyceryl monostearate and mixing evenly, wherein the weight ratio of solid to liquid is 1:8-10, and the mass of glyceryl monostearate is 1-1.5% of the mass of the purple ganoderma lucidum spore oil.

2. The processing method according to claim 1, characterized in that The solid-liquid weight ratio is 1:

9.

3. The processing method according to claim 1, characterized in that The mass of the glyceryl monostearate is 1% of the mass of the ganoderma lucidum spore oil.

4. The processing method according to claim 1, characterized in that The method specifically comprises the following steps: 1) Preparation of Ganoderma lucidum spore oil: breaking the spores of Ganoderma lucidum to a breaking rate of over 90%, granulating, and extracting to obtain Ganoderma lucidum spore oil and Ganoderma lucidum spore granules; 2) Preparation of Purple Ganoderma Lucidum Spore Extract: Soak the Ganoderma Lucidum spore particles after degreasing in step 1) in 100°C water for 10-12 hours, decoct with water 2-3 times, filter each extract separately, combine, concentrate, collect into a paste, and vacuum dry to obtain a Ganoderma Lucidum spore extract dry paste, which is crushed to obtain Purple Ganoderma Lucidum Spore Extract; 3) Preparation of Ganoderma lucidum spore extract suspension: Ganoderma lucidum spore oil and glyceryl monostearate are stirred and mixed at 65-75°C. After the glyceryl monostearate is completely dissolved, Ganoderma lucidum spore extract is added at a solid-to-liquid weight ratio of 1:8-10. The suspension is then magnetically stirred at 65-75°C until the solution is uniform. The suspension is then uniformly ground and mixed using a colloid mill to obtain the suspension.

5. The processing method according to claim 4, characterized in that The cell wall breaking in step 1) is specifically to add the purple Ganoderma lucidum spore powder into a Bailey grinder for cell wall breaking for 30-50 minutes, to achieve a cell wall breaking rate of more than 90%.

6. The processing method according to claim 4, characterized in that After the cell wall breaking in step 1) is completed, granulation is performed using a granulator. The granules are evenly spread on a drying tray for drying. The thickness of the spread is 2 to 4 cm. The drying temperature is 100 to 120° C. and the drying time is 1 to 2 hours.

7. The processing method according to claim 4, characterized in that The extraction in step 1) is to put the granulated Ganoderma lucidum spore powder particles into a CO2 supercritical extraction device, set the extraction pressure to 25-35 MPa, the temperature to 40-50°C, and extract for 3-4 hours to obtain purple Ganoderma lucidum spore oil and Ganoderma lucidum spore particles.

8. The processing method according to claim 4, characterized in that The water in step 2) is decocted twice, the first time with 8-10 times the weight of water for 2-3 hours, and the second time with 6-8 times the weight of water for 1-2 hours.

9. The processing method according to claim 4, characterized in that The colloid mill described in step 3) is used for uniform mixing for 3-8 minutes.

10. The processing method according to claim 4, characterized in that In step 3), the magnetic stirring is continued at 65-75° C. for 30-40 minutes until the solution becomes a homogeneous oil.

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