Carbapenem drug resistance gene detection device and detection method

Abstract

The invention belongs to the technical field of gene detection, and particularly relates to a carbapenem drug resistance gene detection device and method.The carbapenem drug resistance gene detection device comprises a detector, a detection cavity is formed in the detector, a detection unit is arranged in the detection cavity, and the detection unit comprises a pneumatic control module, an optical detection module and a constant temperature module; a sealing cover for sealing the detection cavity is arranged on the top surface of the detector; a micro-fluidic chip is placed on a suction cup, the micro-fluidic chip is pressed to be adsorbed on the suction cup, then a sealing cover is closed, an electric telescopic rod moves downwards, a first spring pushes a connecting plate, the connecting plate drives the suction cup to move downwards, and the suction cup drives the chip to move downwards; when the micro-fluidic chip is placed in the groove, the bottom surface of the chip is in contact with the inner wall of the groove, and then the electric telescopic rod is closed, so that the micro-fluidic chip is fixed in the groove, and the micro-fluidic chips of different models and sizes can be fixed through the suction cup.

Description

Technical Field

[0001] The present invention belongs to the technical field of gene detection, and in particular relates to a carbapenem-resistant gene detection device and a detection method. Background Art

[0002] Currently, bacterial resistance has become a major challenge in the field of global public health. Among them, the infection situation caused by carbapenem-resistant Enterobacteriales (CRE) is the most serious. The production of carbapenemases is the main mechanism of resistance of Enterobacteriales to carbapenems. Accurate and rapid detection and typing of carbapenemases produced by CRE is of great value for precise drug use in clinical anti-infection treatment and prevention and control of hospital infections.

[0003] Microfluidic chip technology has the advantages of being fast, simple, highly specific, highly sensitive, and high-throughput, and is used for bacterial identification. This technology is used to analyze KPC, NDM, IMP, and VIM resistance genes. The sequencing results are used as the gold standard to calculate specificity and sensitivity, providing data for clinical anti-infection treatment.

[0004] When in use, the microfluidic chip needs to be placed in a detection chamber, which integrates three modules: pneumatic control, optical detection and constant temperature. The pneumatic system precisely controls the flow of sample fluid (carbapenem) in the chip; the optical module is responsible for stimulating fluorescence signals and collecting detection data; the constant temperature module maintains the reaction temperature to ensure isothermal amplification of the sample. The modules work together to achieve efficient and accurate detection and analysis.

[0005] However, the above technology often has the following defects: Currently, microfluidic chips are available in a variety of sizes and models, but in actual use, the chip needs to be placed in a chip slot in a detection chamber. The chip slot is often difficult to adapt to chips of different specifications. Since the chip slot is usually a fixed size, when the chip size is too small or too large, the chip cannot be fixed, thereby affecting the limitations of the use of the detection equipment. For this reason, the present invention provides a carbapenem-resistant gene detection device and a detection method. Summary of the Invention

[0006] In order to make up for the deficiencies of the prior art, at least one technical problem raised in the background technology is solved.

[0007] The technical solution adopted by the present invention to solve its technical problems is: a carbapenem-resistant gene detection device described in the present invention includes a detector, a detection cavity is provided in the detector, a detection unit is provided in the detection cavity, the detection unit includes a pneumatic control module, an optical detection module and a constant temperature module, and the top surface of the detector is provided with a sealing cover for sealing the detection cavity; the bottom surface of the inner wall of the detection cavity is fixedly connected to a detection platform, the top surface of the detection platform is provided with a groove, a cavity is provided in the detection platform, and a connecting plate is provided in the cavity; a group of connecting holes communicating with the cavity and the groove are provided on the detection platform, a connecting plate is provided in the cavity, a group of connecting rods passing through the connecting holes are fixedly connected to the top surface of the connecting plate, a suction cup is fixedly connected to the top surface of the connecting rod, an electric telescopic rod for pushing the connecting plate is fixedly connected to the bottom surface of the inner wall of the cavity, a group of first springs are fixedly connected between the top surface of the connecting plate and the inner wall of the cavity, and a microfluidic chip is provided on the suction cup.

[0008] Preferably, a pair of connecting grooves are provided in the detection platform, an air inlet is provided between the inner wall of the cavity and the connecting groove, a plurality of air outlets connected to the connecting groove are provided on the inner wall of the groove, and the connecting plate is sealed and slidably connected to the inner wall of the cavity.

[0009] Preferably, a pair of limit blocks are slidably connected in the detection platform, the bottom surface of the limit block is located in the cavity, the top surface of the limit block is located in the groove, the outer wall of the limit block is fixedly connected to a fixed plate, and a driving spring is fixedly connected between the top surface of the fixed plate and the inner wall of the cavity.

[0010] Preferably, the constant temperature module includes a group of constant temperature rods arranged in the cavity, the constant temperature rods are located above the connecting plate, and the inner wall of the connecting hole is fixedly connected to an elastic sealing ring.

[0011] Preferably, the detection platform is provided with multiple groups of sliding grooves corresponding to the sealing rings, the sliding grooves are sealed and slidably connected with magnetic blocks, the inner wall of the sliding grooves is slidably connected with a magnetic plate that repels the magnetic blocks, a second spring is fixedly connected between the side of the magnetic plate away from the sealing ring and the inner wall of the sliding groove, a connecting line is fixedly connected between the bottom surface of the magnetic plate and the connecting plate, a through groove connected to the sliding groove is provided on the inner wall of the connecting hole, the interior of the sealing ring is a hollow structure, the outer wall of the sealing ring is provided with a through hole corresponding to the through groove, and the inner wall of the cavity is fixedly connected with a positioning plate.

[0012] Preferably, a group of rotatable shafts are provided on the top surface of the connecting plate, a group of driving blades are fixedly connected to the outer side walls of the shafts, and a driving assembly for driving the shafts to rotate is provided on the connecting plate.

[0013] Preferably, the driving assembly includes a rectangular groove opened on the bottom surface of the connecting plate, a rectangular plate is arranged in the rectangular groove, the output end of the electric telescopic rod is fixedly connected to the rectangular plate, the rotating shaft is slidingly connected to the top surface of the connecting plate, and the bottom end of the rotating shaft is rotatably connected to the top surface of the rectangular plate.

[0014] Preferably, the interior of the connecting rod is a hollow structure, the top end of the connecting rod is connected to the suction cup, the top surface of the connecting plate is provided with a circular hole connected to the connecting rod, a piston is sealingly and slidingly connected inside the connecting rod, a first elastic rope is fixedly connected between the bottom surface of the piston and the inner wall of the connecting rod, a second elastic rope is fixedly connected between the top surface of the piston and the inner wall of the connecting rod, the elastic force of the second elastic rope is greater than that of the first elastic rope, and a limiting ring is fixedly connected to the inner wall of the connecting rod.

[0015] A method for detecting carbapenem-resistant genes, using the above-mentioned carbapenem-resistant gene detection device, comprises the following steps: S1: The carbapenem sample is injected into the microfluidic chip, the sealing cover is opened, and the microfluidic chip is placed on the limit block. The output end of the electric telescopic rod and the first spring are then used to control the up and down movement of the connecting plate, allowing the connecting plate to push the gas in the cavity to spray from the outlet to the microfluidic chip; S2: Press the microfluidic chip onto the suction cup, and then move the output end of the electric telescopic rod downward. At this time, the first spring pushes the connecting plate, so that the connecting plate drives the microfluidic chip on the suction cup to contact the inner wall of the groove; S3: The sample is sucked into the inlet of the microfluidic chip through the pneumatic control module, flows through the chamber pre-stored with lysis reagents, and enters the heating zone after mixing. The pneumatic membrane pump drives the mixed liquid through the built-in silica membrane microcolumn or magnetic bead capture area of ​​the chip. The waste liquid is pumped into the waste liquid tank. The purified nucleic acid is bound to the membrane / magnetic beads, and the wash buffer is pumped in for cleaning; S4: The pneumatic membrane pump accurately delivers the eluted nucleic acid solution to multiple parallel PCR micro-reaction pools on the microfluidic chip. At the same time, the pre-mixed PCR master mix is ​​pumped in. The nucleic acid template and reaction reagents are mixed and redissolved in the micro-reaction pools. The PMT does not work to confirm whether the liquid fills the reaction pools. S5: The cavity is heated by the thermostat rod, and the driving blade is rotated at the same time, so that the driving blade stirs the gas above the connecting plate until the temperature in the cavity is heated to 64°C-66°C. At this time, the heat in the cavity acts on the microfluidic chip on the groove to provide a constant temperature effect for the microfluidic chip; S6: The optical module is responsible for stimulating fluorescence signals and collecting test data to complete the detection of carbapenem-resistant genes.

[0016] The beneficial effects of the present invention are as follows: 1. The present invention places the microfluidic chip on the suction cup and presses the microfluidic chip to adsorb on the suction cup. Then, the sealing cover is closed and the electric telescopic rod is moved downward. At this time, the first spring pushes the connecting plate, so that the connecting plate drives the suction cup to move downward. At this time, the suction cup drives the chip downward. At this time, the bottom surface of the chip contacts the inner wall of the groove. Then, the electric telescopic rod is closed to fix the microfluidic chip in the groove. Microfluidic chips of different models and sizes can be fixed through the suction cup.

[0017] 2. When the connecting plate in the present invention is pushed downward by the first spring, the connecting plate will push the gas in the cavity into the connecting groove from the air inlet. At this time, the gas will be blown out from the air outlet to the microfluidic chip, thereby blowing away impurities on the microfluidic chip to improve the accuracy of subsequent detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The present invention will be further described below with reference to the accompanying drawings.

[0019] Figure 1 Schematic diagram of the three-dimensional structure of the detector in the present invention; Figure 2 This is a schematic diagram of the structure of the detector after removing the sealing cover of the present invention; Figure 3 It is a schematic diagram of the three-dimensional structure of the detection platform of the present invention; Figure 4 It is a schematic diagram of the internal structure of the detection platform of the present invention; Figure 5 yes Figure 4 A magnified view of point A; Figure 6 Schematic diagram of the internal structure of the rectangular plate and the connecting rod in the present invention; Figure 7 It is a flow chart of the method in the present invention.

[0020] In the figure: 1. detector; 2. sealing cover; 3. detection chamber; 4. detection platform; 5. groove; 6. microfluidic chip; 7. cavity; 8. connecting plate; 9. connecting rod; 10. suction cup; 11. electric telescopic rod; 12. connecting groove; 13. air outlet; 14. air inlet; 15. limiting block; 16. driving spring; 17. constant temperature rod; 18. sealing ring; 19. positioning plate; 20. magnetic block; 21. through groove; 22. magnetic plate; 23. connecting line; 24. rotating shaft; 25. driving blade; 26. rectangular plate; 27. rectangular groove; 28. first elastic rope; 29. ​​limiting ring; 30. piston; 31. second elastic rope; 32. slide groove. DETAILED DESCRIPTION

[0021] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0022] Example 1: Figures 1 to 5 As shown, a carbapenem-resistant gene detection device according to an embodiment of the present invention includes a detector 1, a detection cavity 3 is provided in the detector 1, a detection unit is provided in the detection cavity 3, and the detection unit includes a pneumatic control module, an optical detection module and a constant temperature module. The top surface of the detector 1 is provided with a sealing cover 2 for sealing the detection cavity 3; the bottom surface of the inner wall of the detection cavity 3 is fixedly connected to a detection platform 4, the top surface of the detection platform 4 is provided with a groove 5, and a cavity 7 is provided in the detection platform 4, and the cavity 7 is provided with a connecting plate 8; a group of connecting holes communicating with the cavity 7 and the groove 5 are opened on the detection platform 4, a connecting plate 8 is provided in the cavity 7, the top surface of the connecting plate 8 is fixedly connected to a group of connecting rods 9 passing through the connecting holes, the top surface of the connecting rods 9 is fixedly connected to a suction cup 10, the bottom surface of the inner wall of the cavity 7 is fixedly connected to an electric telescopic rod 11 for pushing the connecting plate 8, a group of first springs are fixedly connected between the top surface of the connecting plate 8 and the inner wall of the cavity 7, and a microfluidic chip 6 is provided on the suction cup 10; In the present application, when detecting carbapenem-resistant genes, the carbapenem sample can be injected into the microfluidic chip 6, and then the microfluidic chip 6 is placed on the suction cup 10, and the microfluidic chip 6 is pressed to be adsorbed on the suction cup 10, and then the sealing cover 2 is closed. At this time, the electric telescopic rod 11 is moved downward, and the first spring will push the connecting plate 8, so that the connecting plate 8 drives the suction cup 10 to move downward, and the suction cup 10 will drive the chip to move downward. At this time, the bottom surface of the chip will contact the inner wall of the groove 5, and then the electric telescopic rod 11 is closed, thereby fixing the microfluidic chip 6 in the groove 5. Microfluidic chips 6 of different models and sizes can be fixed by the suction cup 10, thereby improving the practicality of the entire device. Then, the pneumatic control module is used to control the flow of the sample in the microfluidic chip 6, and the optical detection module maintains a constant temperature for the microfluidic chip 6. The optical module is responsible for exciting the fluorescence signal and collecting detection data to complete the detection of carbapenem-resistant genes.

[0023] A pair of connecting grooves 12 are provided in the detection platform 4, and an air inlet 14 is provided between the inner wall of the cavity 7 and the connecting groove 12. The inner wall of the groove 5 is provided with multiple groups of air outlet holes 13 connected to the connecting groove 12, and the connecting plate 8 is sealed and slidably connected to the inner wall of the cavity 7; in this application, when the connecting plate 8 is pushed downward by the first spring, the connecting plate 8 will push the gas in the cavity 7 from the air inlet 14 into the connecting groove 12, and at this time the gas will be blown out from the air outlet 13 onto the microfluidic chip 6, thereby blowing away impurities on the microfluidic chip 6 to improve the accuracy of subsequent detection.

[0024] A pair of limit blocks 15 are slidably connected in the detection platform 4, the bottom surface of the limit block 15 is located in the cavity 7, and the top surface of the limit block 15 is located in the groove 5. The outer wall of the limit block 15 is fixedly connected with a fixing plate, and a driving spring 16 is fixedly connected between the top surface of the fixing plate and the inner wall of the cavity 7; when the microfluidic chip 6 in the present application is placed in the groove 5, the microfluidic chip 6 will first contact the limit block 15. At this time, the output end of the electric telescopic rod 11 can be repeatedly moved up and down. When the output end moves downward, the first spring will push the connecting plate 8, allowing the connecting plate 8 to push the gas in the cavity 7 so that the gas can be ejected from the air outlet 13. The upward movement of the output end can push the connecting plate 8 to reset. In the process of the output end moving back and forth, the air outlet 13 can continuously discharge air, thereby continuously blowing and cleaning the microfluidic chip 6 to improve the cleaning effect of the microfluidic chip 6.

[0025] The constant temperature module includes a group of constant temperature rods 17 arranged in the cavity 7, and the constant temperature rods 17 are located above the connecting plate 8. The inner wall of the connecting hole is fixedly connected with an elastic sealing ring 18. Before performing the test, the present application will first adsorb the microfluidic chip 6 on the suction cup 10, and let the bottom surface of the microfluidic chip 6 contact the inner wall of the groove 5. At the same time, the sealing ring 18 will contact and seal with the suction cup 10, so that the cavity 7 is in a sealed state. Then, the cavity 7 can be heated with the help of a heating rod until the temperature in the cavity 7 is heated to 64-66°C. At this time, the heat in the cavity 7 acts on the microfluidic chip 6 on the groove 5 to provide a constant temperature effect on the microfluidic chip 6, thereby providing an effect on genetic testing.

[0026] The detection platform 4 is provided with multiple groups of slide grooves 32 corresponding to the sealing ring 18, and the slide groove 32 is sealed and slidably connected with a magnetic block 20, and the inner wall of the slide groove 32 is slidably connected with a magnetic plate 22 that repels the magnetic block 20, and a second spring is fixedly connected between the side of the magnetic plate 22 away from the sealing ring 18 and the inner wall of the slide groove 32, and a connecting line 23 is fixedly connected between the bottom surface of the magnetic plate 22 and the connecting plate 8, and a through groove 21 communicating with the slide groove 32 is provided on the inner wall of the connecting hole, the interior of the sealing ring 18 is a hollow structure, and the outer wall of the sealing ring 18 is provided with a through hole corresponding to the through groove 21, and the inner wall of the cavity 7 is fixedly connected with a positioning plate 19; When the connecting plate 8 of the present application moves downward, it will drive the suction cup 10 to move downward, so that the suction cup 10 enters the connecting hole and contacts the sealing ring 18. At the same time, the connecting plate 8 will contact the positioning. At the same time, the connecting line 23 will also pull the magnetic plate 22 downward, so that the magnetic plate 22 moves away from the front of the magnetic block 20. At this time, the magnetic block 20 will move under the push of the second spring, so that the gas in the slide groove 32 enters the sealing ring 18 from the through groove 21, so that the sealing ring 18 expands and further fits with the suction cup 10 to improve the sealing effect of the cavity 7. The connecting plate 8 is controlled to move upward by the electric telescopic rod 11, so that the connecting plate 8 can push the magnetic plate 22 to reset. At this time, the magnetic block 20 will reset due to the repulsion with the magnetic plate 22.

[0027] Example 2: Figure 6 As shown, compared with Example 1, another embodiment of the present invention is: a group of rotatable shafts 24 are provided on the top surface of the connecting plate 8, and a group of driving blades 25 are fixedly connected to the outer side wall of the shaft 24, and a driving component for driving the shaft 24 to rotate is provided on the connecting plate 8; when the constant temperature rod 17 heats the cavity 7, the driving component can be used to drive the shaft 24 to rotate. At this time, the driving blades 25 will rotate, so that the driving blades 25 stir the gas above the connecting plate 8, thereby improving the efficiency of the constant temperature rod 17 in heating the gas. At the same time, due to the stirring of the gas, the gas can also be better in a uniform temperature state, thereby improving the constant temperature effect of the microfluidic chip 6.

[0028] The driving assembly includes a rectangular groove 27 opened on the bottom surface of the connecting plate 8, and a rectangular plate 26 is arranged in the rectangular groove 27. The output end of the electric telescopic rod 11 is fixedly connected to the rectangular plate 26, the rotating shaft 24 is slidably connected to the top surface of the connecting plate 8, and the bottom end of the rotating shaft 24 is rotatably connected to the top surface of the rectangular plate 26; after the connecting plate 8 contacts the positioning plate 19, the output end of the electric telescopic rod 11 can control the rectangular plate 26 to continue to move up and down. During the up and down movement of the rectangular plate 26, the driving blade 25 will be pushed to rotate by the gas in the cavity 7, thereby causing the driving blade 25 to rotate to stir and mix the gas in the cavity 7.

[0029] The interior of the connecting rod 9 is a hollow structure, the top of the connecting rod 9 is connected to the suction cup 10, and the top surface of the connecting plate 8 is provided with a circular hole connected to the connecting rod 9. A piston 30 is sealed and slidably connected inside the connecting rod 9, and a first elastic rope 28 is fixedly connected between the bottom surface of the piston 30 and the inner wall of the connecting rod 9, and a second elastic rope 31 is fixedly connected between the top surface of the piston 30 and the inner wall of the connecting rod 9. The elastic force of the second elastic rope 31 is greater than that of the first elastic rope 28, and a limiting ring 29 is fixedly connected to the inner wall of the connecting rod 9; when the rectangular plate 26 in the present application moves downward, the first elastic rope 28 will pull the piston 30 downward. At this time, the piston 30 will suck the gas in the suction cup 10, so that the suction cup 10 can more firmly adsorb the microfluidic chip 6, thereby improving the fixing effect of the microfluidic chip 6. After that, when the rectangular plate 26 continues to move downward, the limiting ring 29 will limit the piston 30.

[0030] like Figure 7 As shown, a method for detecting carbapenem-resistant genes is provided, wherein the method uses the above-mentioned carbapenem-resistant gene detection device and comprises the following steps: S1: The carbapenem sample is injected into the microfluidic chip 6, and the sealing cover 2 is opened. The microfluidic chip 6 is then placed on the limit block 15. The connecting plate 8 is then moved up and down by the cooperation between the output end of the electric telescopic rod 11 and the first spring, so that the connecting plate 8 pushes the gas in the cavity 7 to be sprayed from the gas outlet 13 onto the microfluidic chip 6. S2: Press the microfluidic chip 6 to adsorb on the suction cup 10, and then move the output end of the electric telescopic rod 11 downward. At this time, the first spring pushes the connecting plate 8, so that the connecting plate 8 drives the microfluidic chip 6 on the suction cup 10 to contact the inner wall of the groove 5; S3: The sample is sucked into the inlet of the microfluidic chip 6 through the pneumatic control module, flows through the chamber pre-stored with the lysis reagent, and enters the heating zone after mixing. The pneumatic membrane pump drives the mixed liquid through the built-in silica membrane microcolumn or magnetic bead capture area of ​​the chip. The waste liquid is pumped into the waste liquid tank. The purified nucleic acid is bound to the membrane / magnetic beads, and the washing buffer is pumped in for cleaning; S4: The pneumatic membrane pump accurately delivers the eluted nucleic acid solution to multiple parallel PCR micro-reaction pools of the microfluidic chip 6. At the same time, the pre-mixed PCR master mix is ​​pumped in. The nucleic acid template and reaction reagents are mixed and redissolved in the micro-reaction pools. The PMT does not work to confirm whether the liquid fills the reaction pools. S5: The cavity 7 is heated by the thermostatic rod 17, and the driving blade 25 is rotated, so that the driving blade 25 stirs the gas above the connecting plate 8 until the temperature in the cavity 7 is heated to 64°C-66°C. At this time, the heat in the cavity 7 is applied to the microfluidic chip 6 on the groove 5, thereby providing a constant temperature effect for the microfluidic chip 6; S6: The optical module is responsible for stimulating fluorescence signals and collecting test data to complete the detection of carbapenem-resistant genes.

[0031] Working principle: By injecting the carbapenem sample into the microfluidic chip 6, then placing the microfluidic chip 6 on the suction cup 10, and pressing the microfluidic chip 6 to adsorb on the suction cup 10, then closing the sealing cover 2, at this time, let the electric telescopic rod 11 move downward, at this time the first spring will push the connecting plate 8, so that the connecting plate 8 drives the suction cup 10 to move downward, at this time the suction cup 10 will drive the chip downward, at this time the bottom surface of the chip will contact the inner wall of the groove 5, and then close the electric telescopic rod 11, thereby fixing the microfluidic chip 6 in the groove 5, and the suction cup 10 can be used to fix microfluidic chips 6 of different models and sizes. The pneumatic control module is used to control the sample to flow in the microfluidic chip 6, the optical detection module controls the temperature of the microfluidic chip 6, and the optical module is responsible for exciting the fluorescence signal and collecting the detection data to complete the detection of the carbapenem-resistant gene. In the present application, when the connecting plate 8 is pushed downward by the first spring, the connecting plate 8 pushes the gas in the cavity 7 into the connecting groove 12 from the air inlet 14. At this time, the gas is blown out from the air outlet 13 onto the microfluidic chip 6, thereby blowing away the impurities on the microfluidic chip 6 to improve the accuracy of subsequent detection. When the microfluidic chip 6 in the present application is placed in the groove 5, the microfluidic chip 6 will first contact the limit block 15. At this time, the output end of the electric telescopic rod 11 can be repeatedly moved up and down. When the output end moves downward, the first spring pushes the connecting plate 8, allowing the connecting plate 8 to push the gas in the cavity 7 so that the gas can be ejected from the air outlet 13. The upward movement of the output end can push the connecting plate 8 to reset. During the back-and-forth movement of the output end, the air outlet 13 can continuously discharge air, thereby continuously blowing and cleaning the microfluidic chip 6, thereby improving the cleaning effect of the microfluidic chip 6; Before the test is carried out, the present application will first adsorb the microfluidic chip 6 on the suction cup 10, and make the bottom surface of the microfluidic chip 6 contact the inner wall of the groove 5. At the same time, the sealing ring 18 will contact and seal with the suction cup 10, so that the cavity 7 is in a sealed state. Then, the cavity 7 can be heated with the help of a heating rod until the temperature in the cavity 7 is heated to 64-66°C. At this time, the heat in the cavity 7 acts on the microfluidic chip 6 on the groove 5 to provide a constant temperature effect for the microfluidic chip 6, thereby providing an effect for genetic testing; when the connecting plate 8 of the present application moves downward, it will drive the suction cup 10 to move downward, so that the suction cup 10 enters At this time, the magnetic block 20 will move under the push of the second spring, so that the gas in the slide groove 32 enters the sealing ring 18 from the through groove 21, thereby allowing the sealing ring 18 to expand and further fit the suction cup 10 to improve the sealing effect of the cavity 7. The connecting plate 8 is controlled to move upward by the electric telescopic rod 11, so that the connecting plate 8 can push the magnetic plate 22 to reset. At this time, the magnetic block 20 will reset due to the repulsion with the magnetic plate 22.

[0032] The above-mentioned front, back, left, right, up and down are all based on the Figure 1 As a benchmark, according to the person's observation perspective, the side of the device facing the observer is defined as the front, the left side of the observer is defined as the left, and so on.

[0033] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the scope of protection of the present invention.

[0034] The basic principles, main features, and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the foregoing embodiments. The foregoing embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A carbapenem-resistant gene detection device, comprising a detector (1), wherein a detection cavity (3) is provided in the detector (1), wherein a detection unit is provided in the detection cavity (3), wherein the detection unit comprises a pneumatic control module, an optical detection module and a constant temperature module, and a sealing cover (2) is provided on the top surface of the detector (1) for sealing the detection cavity (3); Its characteristics are: The bottom surface of the inner wall of the detection cavity (3) is fixedly connected to a detection platform (4), a groove (5) is provided on the top surface of the detection platform (4), a cavity (7) is provided in the detection platform (4), and a connecting plate (8) is provided in the cavity (7); The detection platform (4) is provided with a group of connection holes communicating with the cavity (7) and the groove (5); a connection plate (8) is provided in the cavity (7); a group of connection rods (9) passing through the connection holes are fixedly connected to the top surface of the connection plate (8); a suction cup (10) is fixedly connected to the top surface of the connection rod (9); an electric telescopic rod (11) for pushing the connection plate (8) is fixedly connected to the bottom surface of the inner wall of the cavity (7); a group of first springs are fixedly connected between the top surface of the connection plate (8) and the inner wall of the cavity (7); and a microfluidic chip (6) is provided on the suction cup (10).

2. The carbapenem resistance gene detection device according to claim 1, characterized in that: A pair of connecting grooves (12) are provided in the detection platform (4), an air inlet (14) is provided between the inner wall of the cavity (7) and the connecting groove (12), a plurality of air outlets (13) are provided on the inner wall of the groove (5) and are connected to the connecting groove (12), and the connecting plate (8) is sealingly and slidingly connected to the inner wall of the cavity (7).

3. The carbapenem resistance gene detection device according to claim 2, characterized in that: A pair of limit blocks (15) are slidably connected in the detection platform (4), the bottom surface of the limit block (15) is located in the cavity (7), the top surface of the limit block (15) is located in the groove (5), the outer wall of the limit block (15) is fixedly connected to a fixed plate, and a driving spring (16) is fixedly connected between the top surface of the fixed plate and the inner wall of the cavity (7).

4. The carbapenem resistance gene detection device according to claim 3, characterized in that: The constant temperature module comprises a group of constant temperature rods (17) arranged in the cavity (7), the constant temperature rods (17) are located above the connecting plate (8), and an elastic sealing ring (18) is fixedly connected to the inner wall of the connecting hole.

5. The carbapenem resistance gene detection device according to claim 4, characterized in that: The detection platform (4) is provided with a plurality of groups of slide grooves (32) corresponding to the sealing ring (18), the slide groove (32) is sealed and slidably connected with a magnetic block (20), the inner wall of the slide groove (32) is slidably connected with a magnetic plate (22) that repels the magnetic block (20), a second spring is fixedly connected between the side of the magnetic plate (22) away from the sealing ring (18) and the inner wall of the slide groove (32), a connecting line (23) is fixedly connected between the bottom surface of the magnetic plate (22) and the connecting plate (8), the inner wall of the connecting hole is provided with a through groove (21) connected with the slide groove (32), the interior of the sealing ring (18) is a hollow structure, the outer wall of the sealing ring (18) is provided with a through hole corresponding to the through groove (21), and the inner wall of the cavity (7) is fixedly connected with a positioning plate (19).

6. The carbapenem-resistant gene detection device according to claim 5, characterized in that: A group of rotatable shafts (24) are provided on the top surface of the connecting plate (8), a group of driving blades (25) are fixedly connected to the outer side wall of the shaft (24), and a driving assembly for driving the shaft (24) to rotate is provided on the connecting plate (8).

7. The carbapenem-resistant gene detection device according to claim 6, characterized in that: The driving assembly includes a rectangular groove (27) provided on the bottom surface of the connecting plate (8), a rectangular plate (26) is provided in the rectangular groove (27), the output end of the electric telescopic rod (11) is fixedly connected to the rectangular plate (26), the rotating shaft (24) is slidably connected to the top surface of the connecting plate (8), and the bottom end of the rotating shaft (24) is rotatably connected to the top surface of the rectangular plate (26).

8. The carbapenem-resistant gene detection device according to claim 7, characterized in that: The interior of the connecting rod (9) is a hollow structure. The top end of the connecting rod (9) is connected to the suction cup (10). The top surface of the connecting plate (8) is provided with a circular hole connected to the connecting rod (9). A piston (30) is sealed and slidably connected inside the connecting rod (9). A first elastic rope (28) is fixedly connected between the bottom surface of the piston (30) and the inner wall of the connecting rod (9). A second elastic rope (31) is fixedly connected between the top surface of the piston (30) and the inner wall of the connecting rod (9). The elastic force of the second elastic rope (31) is greater than that of the first elastic rope (28). A limiting ring (29) is fixedly connected to the inner wall of the connecting rod (9).

9. A method for detecting carbapenem-resistant genes, the method using the carbapenem-resistant gene detection device of claim 8, characterized in that: The method comprises the following steps: S1: injecting a carbapenem sample into the microfluidic chip (6), then opening the sealing cover (2), placing the microfluidic chip (6) on the limit block (15), and then controlling the connection plate (8) to move up and down by means of the output end of the electric telescopic rod (11) and the first spring, so that the connection plate (8) pushes the gas in the cavity (7) to spray onto the microfluidic chip (6) from the gas outlet (13); S2: Press the microfluidic chip (6) to be adsorbed on the suction cup (10), and then move the output end of the electric telescopic rod (11) downward. At this time, the first spring pushes the connecting plate (8), so that the connecting plate (8) drives the microfluidic chip (6) on the suction cup (10) to contact the inner wall of the groove (5); S3: The sample is sucked into the inlet of the microfluidic chip (6) through the pneumatic control module, flows through the chamber where the lysis reagent is pre-stored, and enters the heating zone after mixing. The pneumatic membrane pump drives the mixed liquid through the built-in silica membrane microcolumn or magnetic bead capture area of ​​the chip. The waste liquid is pumped into the waste liquid tank, the purified nucleic acid is bound to the membrane / magnetic beads, and the washing buffer is pumped in for cleaning.

10. The method for detecting carbapenem-resistant genes according to claim 9, characterized in that: The method further comprises the steps of: S4: The pneumatic membrane pump accurately delivers the eluted nucleic acid solution to multiple parallel PCR micro-reaction pools of the microfluidic chip (6). At the same time, the pre-mixed PCR master mix is ​​pumped in. The nucleic acid template and the reaction reagent are mixed and redissolved in the micro-reaction pool. The PMT does not work to confirm whether the liquid fills the reaction pool. S5: The cavity (7) is heated by the thermostatic rod (17), and the driving blade (25) is rotated at the same time, so that the driving blade (25) stirs the gas above the connecting plate (8) until the temperature in the cavity (7) is heated to 64°C-66°C. At this time, the heat in the cavity (7) acts on the microfluidic chip (6) on the groove (5), thereby providing a constant temperature effect for the microfluidic chip (6); S6: The optical module is responsible for stimulating fluorescence signals and collecting test data to complete the detection of carbapenem-resistant genes.

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