Composition with permeation enhancing effect and application thereof

Abstract

The invention relates to a composition with a permeation enhancing effect and application thereof. The composition is prepared from the following components in parts by weight: 10 to 15 parts of ethoxydiethylene glycol, 10 to 15 parts of isosorbide dimethyl ether, 5 to 10 parts of folium artemisiae argyi extract and 1 to 6 parts of tea tree essential oil. According to the permeation enhancing composition, traditional azone easy to sensitize and ethyl alcohol easy to cause stimulation are abandoned, the efficient, safe and lasting permeation effect is finally achieved, and the permeation enhancing composition meets the high-activity component delivery requirement of cosmetics.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition with penetration-promoting effect and application thereof. Background Art

[0002] In the current field of cosmetic technology, there are many types of commonly used penetration enhancers, mainly including surfactants (such as cationic, anionic, nonionic surfactants and lecithin), azone compounds, alcohol compounds (such as ethanol, propylene glycol, etc.), fatty alcohols or fatty acids (such as oleic acid, linoleic acid and lauryl alcohol, etc.), keratin moisturizers (such as urea, salicylic acid, etc.), terpene compounds (such as menthol, brain, limonene, etc.).

[0003] However, in the actual cosmetics production process, commonly used penetration enhancers are often relatively simple in composition, typically employing only one or a single class of penetration enhancers. This results in an inability to enhance the product's penetration across multiple dimensions, resulting in unsatisfactory overall penetration performance. Furthermore, the application of existing penetration enhancers fails to fully consider the skin's absorption and utilization of the active ingredients in cosmetics. For cosmetics, good transdermal performance is as important as the skin's absorption of the active ingredients; both determine the ultimate skin improvement effect, making both penetration enhancement and absorption indispensable.

[0004] In view of this, there is an urgent need to develop new penetration-enhancing technologies in this field to significantly improve the transdermal and absorption effects of cosmetics through comprehensive multi-dimensional means. Summary of the Invention

[0005] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a composition with penetration-enhancing effect and its application.

[0006] To achieve the above object, the technical solution adopted by the present invention is: In a first aspect, the present invention provides a composition having a penetration-enhancing effect, comprising the following components in parts by weight: 10-15 parts of ethoxydiglycol, 10-15 parts of dimethyl isosorbide, 5-10 parts of mugwort leaf extract, and 1-6 parts of tea tree essential oil; wherein the preparation method of the mugwort leaf extract comprises the following steps: S1. Grind the wormwood leaves, soak them in deionized water, and extract them by water distillation to obtain wormwood oil; S2. Inoculating the composite fermentation bacterial liquid into a fermentation medium for fermentation to obtain a fermentation liquid; wherein the composite fermentation bacterial liquid comprises a spherical Lysinibacillus liquid, a Pseudomonas syringae liquid, and a Galacto-Saccharomyces-like bacterial liquid; S3. Adding the mugwort oil obtained in step S1 to the fermentation liquid obtained in step S2 to continue fermentation and cultivation, breaking the emulsion after obtaining the fermentation product, separating the water and oil phases, centrifuging, and taking the upper oil phase to obtain the mugwort extract.

[0007] The ethoxydiglycol of the present invention is a hydrophilic small molecule alcohol ether with good water solubility and skin compatibility. It causes slight disorder of the lipid structure of the stratum corneum, increases the fluidity of the stratum corneum, and provides a penetration channel for other components. Isosorbide dimethyl ether is a cyclic ether compound with strong lipophilicity, can dissolve fat-soluble components, and promote their penetration through the skin lipid barrier. Ethoxydiglycol and isosorbide dimethyl ether can take into account the dissolution and penetration of both hydrophilic and lipophilic components, forming a "water-lipid two-phase penetration-promoting system" and improving the skin penetration efficiency of the entire component.

[0008] Tea tree essential oil contains terpenes, which can affect the lipid molecular structure of the stratum corneum and reduce the resistance of the skin barrier; its fat-soluble characteristics can cooperate with dimethyl isosorbide to enhance its affinity to the stratum corneum of the skin and assist other ingredients in penetrating the lipid barrier.

[0009] The mechanism of each step in the preparation method of the wormwood extract of the present invention is as follows: S1: Use water distillation to extract mugwort oil: The volatile oils in mugwort (such as volatile terpenes and phenols) are volatile. During water distillation, the water vapor carries the volatile oils to the condensation system to form an oil-water mixture, and finally the mugwort oil is separated to provide the basic raw materials for subsequent fermentation.

[0010] S2: Preparation of Fermented Artemisia annua Oil: Lipase produced by Lysinibacillus sphaericus breaks down macromolecular lipids (such as triglycerides and phospholipids) in artemisia annua oil into small short-chain fatty acids and glycerol, making it easier to penetrate the lipid bilayer of the stratum corneum and improving skin moisturizing. Short-chain fatty acids are anti-inflammatory factors that inhibit the release of IL-6 and TNF-α, reducing erythema and stinging. During the fermentation process, Pseudomonas syringae secretes large amounts of surfactants, such as cyclic lipopeptides. These cyclic lipopeptides spontaneously emulsify artemisia annua oil into an O / W nanoemulsion, effectively reducing interfacial tension and allowing oil droplets to more easily penetrate microcracks in the stratum corneum. Cutinases and proteases produced by Galacto-like yeasts can mildly hydrolyze keratin in the stratum corneum, creating transient "micropores." Simultaneously, organic acids (lactic acid and citric acid) produced by bacterial metabolism lower the local pH to 4.5–5.0, further softening the stratum corneum and enhancing penetration. The metabolites obtained by fermentation of the above three fermentation bacteria have multiple synergistic effects, such as producing surfactants to optimize dispersibility, decomposing large molecules to increase the proportion of small molecules, reducing irritation, and maintaining skin permeability, which ultimately significantly improves the penetration-enhancing effect of mugwort extract.

[0011] Preferably, in the preparation step S1 of the mugwort leaf extract, the amount of deionized water added is 8-10 times the total mass of the mugwort leaf, and the soaking time is 3-5 hours.

[0012] Preferably, in the preparation step S2 of the mugwort extract, the inoculation amount of the composite fermentation bacteria liquid is 1-5% v / v, and the number of viable bacteria in the composite fermentation bacteria liquid is 3×10 9 -3×10 10 CFU / mL, the ratio of the number of live bacteria of spherical Lysinibacillus liquid, Pseudomonas syringae liquid and Galacto-Saccharomyces-like bacterial liquid in the composite fermentation bacterial liquid is (1-2): (1-3): 1, the fermentation temperature is 30-35°C, and the time is 15-18h.

[0013] In the preparation step S3 of the mugwort extract, the volume ratio of the mugwort oil to the fermentation medium is (2-3):1, the temperature for continued fermentation culture is 30-35°C and the time is 18-24 hours, and the demulsification is performed by adding hydrochloric acid solution and sodium chloride to the fermentation product for demulsification, the mass concentration of the hydrochloric acid solution is 15-20%, and the mass ratio of the fermentation product, hydrochloric acid solution and sodium chloride is 1:(0.01-0.05):(0.1-0.3).

[0014] Preferably, the mass ratio of the mugwort extract to the tea tree essential oil is (7-9): (2-4).

[0015] In a second aspect, the present invention provides use of the composition having penetration-enhancing efficacy in the first aspect in the preparation of cosmetics.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The wormwood extract in the composition of the present invention is obtained by fermenting wormwood oil. wormwood oil itself contains more terpenoid compounds, which can change the keratin conformation, properties, and binding force between relaxed keratin in stratum corneum cells, form microporous channels, and increase the permeability of effective ingredients through intracellular pathways; after fermentation treatment, the macromolecular substances in wormwood oil are decomposed into small molecular substances, and biosurfactants are also generated. The penetration-promoting effect of wormwood extract is significantly improved, while reducing irritation to the skin and improving bioavailability. Ethoxydiglycol, dimethyl isosorbide, wormwood extract and tea tree essential oil have a synergistic effect and have penetration-promoting and antioxidant effects. The penetration-promoting composition of the present invention abandons traditional azone that is easily sensitized and ethanol that is easily irritated, and ultimately achieves an efficient, safe and lasting penetration effect, which is suitable for the delivery of high-active ingredients in cosmetics. DETAILED DESCRIPTION

[0017] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0018] The sources of raw materials and related information used in the following examples and comparative examples are as follows: Ethoxydiglycol: CAS number is 111-90-0; Isosorbide dimethyl ether: CAS number is 5306-85-4; Artemisia argyi: Qi Artemisia argyi (newly harvested that year), purchased from Qichun County, Hubei Province; Tea tree essential oil: purchased from Firmenich Fragrances (China) Co., Ltd., model number is tea tree essential oil 957260; Lysinibacillus sphaeroides: purchased from the General Microbiology Center of China Culture Collection Administration of Microorganisms, with the accession number: CGMCC 1.2410; Pseudomonas syringae: purchased from the General Microbiology Center of China Culture Collection Administration, with the accession number: CGMCC 1.3333; Galactozyme-like bacteria: purchased from Guangdong Provincial Microbial Culture Collection Center, collection number: GDMCC NO: 64235.

[0019] Unless otherwise specified, other materials, reagents, etc. used in the Examples and Comparative Examples can be obtained from commercial sources.

[0020] Example 1 A composition with penetration-enhancing effect, comprising the following components in parts by weight: 12 parts of ethoxydiglycol, 14 parts of isosorbide dimethyl ether, 8 parts of mugwort leaf extract, and 3 parts of tea tree essential oil; The preparation method of the wormwood extract comprises the following steps: S1, after crushing the wormwood leaves, adding deionized water to soak, and extracting by water distillation to obtain wormwood oil; wherein, the amount of deionized water added is 9 times the total mass of the wormwood leaves, and the soaking time is 4h; S2. Inoculating the composite fermentation broth into a fermentation medium for fermentation to obtain a fermentation broth; wherein the composite fermentation broth comprises a spherical lysine Bacillus lysine broth, a lilac Pseudomonas syringae broth, and a galactosidase-like bacterial broth; wherein the inoculation amount of the composite fermentation broth is 3% v / v, and the number of viable bacteria in the composite fermentation broth is 8×10 9 CFU / mL, the ratio of the viable bacteria count of spherical Lysinibacillus solution, Pseudomonas syringae solution and Galacto-Saccharomyces-like bacteria solution in the composite fermentation bacteria solution is 1.8:2.5:1, the fermentation temperature is 32°C and the time is 16 hours; the formula of the fermentation medium is: 18g / L glucose, 7g / L yeast extract and 15g / L rice; S3. Add the mugwort oil obtained in step S1 to the fermentation liquid obtained in step S2 and continue fermentation and cultivation. After obtaining the fermentation product, demulsification is carried out to separate the water and oil phases, centrifuge, and take the upper oil phase to obtain the mugwort extract; wherein, the volume ratio of the mugwort oil to the fermentation medium is 2.5:1, the temperature for continuing fermentation and cultivation is 32°C, and the time is 20 hours. The demulsification is carried out by adding hydrochloric acid solution and sodium chloride to the fermentation product for demulsification, the mass concentration of the hydrochloric acid solution is 18%, and the mass ratio of the fermentation product, hydrochloric acid solution and sodium chloride is 1:0.03:0.2.

[0021] Example 2 A composition with penetration-enhancing effect, comprising the following components in parts by weight: 10 parts of ethoxydiglycol, 10 parts of isosorbide dimethyl ether, 7 parts of mugwort leaf extract, and 2 parts of tea tree essential oil; The preparation method of the wormwood extract comprises the following steps: S1. After crushing the wormwood leaves, add deionized water and soak them, and use water distillation to extract the wormwood oil; wherein the amount of deionized water added is 8 times the total mass of the wormwood leaves, and the soaking time is 3h; S2. Inoculating the composite fermentation broth into a fermentation medium for fermentation to obtain a fermentation broth; wherein the composite fermentation broth comprises a spherical lysinophilic Bacillus lysine broth, a Pseudomonas syringae broth, and a galactosidase-like bacterial broth; wherein the inoculation amount of the composite fermentation broth is 1% v / v, and the number of viable bacteria in the composite fermentation broth is 3×10 10 CFU / mL, the ratio of the viable bacteria count of spherical Lysinibacillus solution, Pseudomonas syringae solution and Galacto-Saccharomyces-like bacteria solution in the composite fermentation bacteria solution is 1:3:1, the fermentation temperature is 30°C and the time is 18 hours; the formula of the fermentation medium is: 18g / L glucose, 7g / L yeast extract and 15g / L rice; S3. Add the mugwort oil obtained in step S1 to the fermentation liquid obtained in step S2 and continue fermentation and cultivation. After obtaining the fermentation product, demulsification is carried out to separate the water and oil phases, centrifuge, and take the upper oil phase to obtain the mugwort extract; wherein, the volume ratio of the mugwort oil to the fermentation medium is 2:1, the temperature for continuing fermentation and cultivation is 30°C, and the time is 24 hours. The demulsification is carried out by adding hydrochloric acid solution and sodium chloride to the fermentation product for demulsification, the mass concentration of the hydrochloric acid solution is 20%, and the mass ratio of the fermentation product, hydrochloric acid solution and sodium chloride is 1:0.01:0.1.

[0022] Example 3 A composition with penetration-enhancing effect, comprising the following components in parts by weight: 15 parts of ethoxydiglycol, 15 parts of isosorbide dimethyl ether, 9 parts of mugwort leaf extract, and 4 parts of tea tree essential oil; The preparation method of the wormwood extract comprises the following steps: S1. After crushing the wormwood leaves, add deionized water and soak them, and use water distillation to extract the wormwood oil; wherein the amount of deionized water added is 10 times the total mass of the wormwood leaves, and the soaking time is 3h; S2. Inoculating the composite fermentation broth into a fermentation medium for fermentation to obtain a fermentation broth; wherein the composite fermentation broth comprises a spherical lysine Bacillus lysine broth, a lilac Pseudomonas syringae broth, and a galactosidase-like bacterial broth; wherein the inoculation amount of the composite fermentation broth is 5% v / v, and the number of viable bacteria in the composite fermentation broth is 3×10 9 CFU / mL, the ratio of the viable bacteria count of spherical Lysinibacillus solution, Pseudomonas syringae solution and Galacto-Saccharomyces-like bacteria solution in the composite fermentation bacteria solution is 2:1:1, the fermentation temperature is 35°C and the time is 15 hours; the formula of the fermentation medium is: 18g / L glucose, 7g / L yeast extract and 15g / L rice; S3. Add the mugwort oil obtained in step S1 to the fermentation liquid obtained in step S2 and continue fermentation and cultivation. After obtaining the fermentation product, demulsification is carried out to separate the water and oil phases, centrifuge, and take the upper oil phase to obtain the mugwort extract; wherein, the volume ratio of the mugwort oil to the fermentation medium is 3:1, the temperature for continuing fermentation and cultivation is 35°C, and the time is 18 hours. The demulsification is carried out by adding hydrochloric acid solution and sodium chloride to the fermentation product for demulsification, the mass concentration of the hydrochloric acid solution is 15%, and the mass ratio of the fermentation product, hydrochloric acid solution and sodium chloride is 1:0.05:0.3.

[0023] Example 4 The only difference between Example 4 and Example 1 is that: a composition with penetration-enhancing effect comprises the following components in parts by weight: 12 parts of ethoxydiglycol, 14 parts of dimethyl isosorbide, 10 parts of mugwort leaf extract and 1 part of tea tree essential oil.

[0024] Example 5 The only difference between Example 5 and Example 1 is that: a composition with penetration-enhancing effect comprises the following components in parts by weight: 12 parts of ethoxydiglycol, 14 parts of dimethyl isosorbide, 5 parts of mugwort leaf extract and 6 parts of tea tree essential oil.

[0025] Comparative Example 1 The only difference between Comparative Example 1 and Example 1 is that: Comparative Example 1 does not add ethoxydiglycol, and uses isosorbide dimethyl ether, mugwort extract and tea tree essential oil with a mass ratio of 14:8:3 to make up for the missing amount.

[0026] Comparative Example 2 The only difference between Comparative Example 2 and Example 1 is that: Comparative Example 2 does not add dimethyl isosorbide, and uses ethoxydiglycol, mugwort extract and tea tree essential oil in a mass ratio of 12:8:3 to make up for the missing amount.

[0027] Comparative Example 3 The only difference between Comparative Example 3 and Example 1 is that: Comparative Example 3 does not add tea tree essential oil, and uses ethoxydiglycol, isosorbide dimethyl ether and mugwort extract in a mass ratio of 12:14:8 to make up for the missing amount.

[0028] Comparative Example 4 The only difference between Comparative Example 4 and Example 1 is that: Comparative Example 4 does not add mugwort extract, and uses ethoxydiglycol, isosorbide dimethyl ether and tea tree essential oil with a mass ratio of 12:14:3 to make up for the missing amount.

[0029] Comparative Example 5 The only difference between Comparative Example 5 and Example 1 is that: in Comparative Example 5, no mugwort extract is added, and an equal amount of mugwort oil is used instead of the mugwort extract. The mugwort oil is prepared by the preparation step S1 of the mugwort extract in Example 1.

[0030] Comparative Example 6 The only difference between Comparative Example 6 and Example 1 is that in the preparation step S2 of the mugwort extract of Comparative Example 6, the composite fermentation bacterial liquid does not add spherical lysine Bacillus liquid, and the total viable bacteria count is supplemented by using a Pseudomonas syringae liquid and a galactosidase-like bacterial liquid with a viable bacteria ratio of 2.5:1.

[0031] Comparative Example 7 The only difference between Comparative Example 7 and Example 1 is that in the preparation step S2 of the mugwort extract of Comparative Example 7, the composite fermentation bacteria liquid is not added with Pseudomonas syringae liquid, and the total viable bacteria count is supplemented by spherical Lysinophilus Bacillus liquid and Galacto-Saccharomyces-like bacteria liquid with a viable bacteria count ratio of 1.8:1.

[0032] Comparative Example 8 The only difference between Comparative Example 8 and Example 1 is that in the preparation step S2 of the mugwort extract of Comparative Example 8, the composite fermentation bacterial liquid does not add a galactose yeast-like bacterial liquid, and the total viable bacterial count is supplemented by a spherical lysine Bacillus liquid and a Pseudomonas syringae liquid with a viable bacterial count ratio of 1.8:2.5.

[0033] Performance Testing 1. Transdermal test In order to verify the penetration-enhancing effect of the composition of the present invention, each group of penetration-enhancing compositions was mixed with ascorbyl tetraisopalmitate in a mass ratio of 9:1 to obtain each sample group. The ascorbyl tetraisopalmitate in the sample was accurately quantified by GC-MS (gas chromatography-mass spectrometry). If its transdermal amount was significantly increased, it could directly prove the penetration-enhancing effect of the penetration-enhancing composition.

[0034] Experimental animals: 52 male ICR mice (20 ± 2 g) were purchased from Beijing Weitonglihua Laboratory Animal Technology Co., Ltd.

[0035] This test example evaluated the in vitro transdermal efficacy of the compositions of Examples 1-5 and Comparative Examples 1-8 using a transdermal diffusion tester. Healthy male ICR mice with intact skin were anesthetized with 2wt% chloral hydrate, their abdominal hair removed, and they were sacrificed by cervical dislocation. The lower abdominal skin was excised, and excess fat and mucous tissue were carefully removed. The skin was then washed and placed in physiological saline and stored at -20°C until use. Thirteen experimental groups (i.e., the compositions of Examples 1-5 and Comparative Examples 1-8) were set up, with four mice in each group.

[0036] After thawing at room temperature, the ex vivo mouse skin was fixed between the upper and lower chambers of a Franz diffusion cell, with the stratum corneum facing upward. The upper chamber was the supply chamber, into which 0.5 g of each group of test samples was added. The lower chamber was the receiving cell, and the receiving solution in the receiving cell was 30% ethanol saline, which was in contact with the dermis of the skin. A magnetic stirring bar was built into the receiving cell. The in vitro transdermal experiment was performed at a stirring speed of 300 rpm and a water bath temperature of (37 ± 1) °C. 0.2 mL of sample was taken every 1 h from 1 to 12 h, and the sample was immediately supplemented with an equal volume of fresh blank receiving solution at the same temperature. The cumulative permeation per unit area (Q) over 12 h was measured. 12 ).

[0037] The calculation formula is as follows:

[0038] Where C n is the drug (ascorbyl tetraisopalmitate) concentration at the nth sampling point (μg / mL), C i is the drug (ascorbyl tetraisopalmitate) concentration (μg / mL) at the i-th sampling point, the value of i ranges from the first sampling point (i = 1) to the second to last sampling point (i = n-1), and A is the effective drug delivery area (3.14 cm 2 ), V is the volume of the receiving solution (mL). The results were averaged, as shown in Table 1.

[0039] Table 1 Cumulative permeation per unit area Q of ascorbyl tetraisopalmitate in each group of samples over 12 hours 12 Group / Performance <![CDATA[抗坏血酸四异棕榈酸酯的Q 12 (μg / cm 2 )]]> Example 1 43.61 Example 2 43.13 Example 3 43.58 Example 4 41.95 Example 5 41.07 Comparative Example 1 30.61 Comparative Example 2 29.97 Comparative Example 3 33.84 Comparative Example 4 32.22 Comparative Example 5 35.07 Comparative Example 6 37.49 Comparative Example 7 36.73 Comparative Example 8 37.25 The greater the cumulative permeation amount per unit area of ​​ascorbyl tetraisopalmitate in the sample over 12 hours, the better the transdermal performance of the sample, which means that the permeation-enhancing performance of the permeation-enhancing composition is better.

[0040] As shown in Table 1, combining the data from Examples 1 and 4-5, it can be seen that the transdermal performance of ascorbyl tetraisopalmitate in the samples of Examples 4-5 is lower than that of Example 1. This may be because: in Example 4, the amount of mugwort extract is excessive and the amount of tea tree essential oil is insufficient. The terpene components of the tea tree essential oil (such as terpinen-4-ol) and ethoxydiglycol synergistically solubilize the fat-soluble active ingredient (ascorbyl tetraisopalmitate), resulting in a poor transdermal effect. In Example 5, the amount of tea tree essential oil is excessive and the amount of mugwort extract is insufficient. The active terpene penetration-enhancing substances in the mugwort extract are lacking, resulting in a decrease in penetration-enhancing performance.

[0041] Combining the data of Example 1 and Comparative Examples 1-4, it can be seen that the performance of Comparative Examples 1-4 is significantly lower than that of Example 1. This may be because: Comparative Example 1 lacks ethoxydiglycol to dissolve intercellular lipids, reducing the penetration path; Comparative Example 2 lacks the solubility of dimethyl isosorbide (DMIS) for the active ingredient, thereby affecting the penetration-enhancing effect; Comparative Example 3 lacks mugwort extract, that is, it lacks the terpenoid compounds and biosurfactants in the mugwort extract, thereby causing the penetration-enhancing effect to decrease; Comparative Example 4 lacks tea tree essential oil, that is, it lacks the terpenoid small molecules in the essential oil, resulting in a decrease in the ability of the active substance to penetrate the stratum corneum. The four components in the composition of the present invention synergistically improve the penetration effect of the active ingredient. When any one of ethoxydiglycol, dimethyl isosorbide, mugwort extract and tea tree essential oil is missing, the penetration-enhancing effect of the composition decreases.

[0042] Combining the data of Example 1 and Comparative Examples 5-8, it can be seen that the performance of Comparative Examples 5-8 is significantly lower than that of Example 1. This may be because the mugwort oil in Comparative Example 5 has not undergone a fermentation step and lacks the organic acids and enzymes produced by fermentation, which cannot effectively decompose macromolecules and generate surfactants, and the permeability is significantly reduced. Comparative Examples 6-8 are composite bacterial agents used for fermentation in mugwort extracts that lack any bacterial species, and their performance is significantly reduced, indicating that the metabolites obtained by fermentation of mugwort oil in the mugwort extract of the present invention by lysine bacillus spherical, Pseudomonas syringae and galactosycoses are synergistically produced by producing surfactants to optimize dispersibility, decompose macromolecules to increase the proportion of small molecules, reduce irritation and maintain skin permeability, and ultimately significantly improve the penetration effect of mugwort oil.

[0043] 2. DPPH inhibition rate test The free radical scavenging ability of the extracts was determined using the DPPH free radical scavenging method. Methanol was used as a blank solution and a DPPH solution as a control, and absorbance was measured at 517 nm. The compositions of Examples 1-5 and Comparative Examples 5-8 were prepared as 1% aqueous solutions as the test samples, and the free radical scavenging ability was calculated using the following formula.

[0044] DPPH inhibition rate (%) = 1-[(OD 样品- OD空白 ) / (OD 对照- OD 空白 )]×100%. In the formula, OD 对照 is the absorbance of the DPPH solution, OD 空白 is the absorbance of the pure methanol solution, and OD 样品 is the absorbance of the DPPH solution added with the extract of different concentrations. The specific data are shown in Table 2.

[0045] Table 2: DPPH inhibition rates of samples in each group Group / Performance DPPH inhibition rate / % Example 1 94.31 Example 2 92.80 Example 3 93.72 Example 4 90.22 Example 5 92.15 Comparative Example 5 70.31 Comparative Example 6 81.46 Comparative Example 7 82.03 Comparative Example 8 81.29 The higher the DPPH inhibition rate is, the better the antioxidant effect of the sample is.

[0046] As shown in Table 2, the composition has a good antioxidant effect. In combination with the data of Example 1 and Examples 4-5, it can be known that the antioxidant effects of Examples 4-5 are all lower than that of Example 1. In combination with the antioxidant effect and cost of the composition, it can be known that the mass ratio of the folium artemisiae argyi extract and the tea tree essential oil should be in the range of (7-9):(2-4).

[0047] In combination with the data of Example 1 and Comparative Examples 5-8, it can be known that the antioxidant performances of Comparative Examples 5-8 are all lower than that of Example 1. This can be because: the folium artemisiae argyi oil of Comparative Example 5 is not subjected to fermentation treatment, and the free flavonoids and the active ingredients enhanced by fermentation are missing, so that the antioxidant performance of the composition is reduced. The folium artemisiae argyi extract obtained by fermenting the folium artemisiae argyi oil with P. globiforme, P. syringae and S. galactosum can effectively improve the antioxidant effect of the composition, while the absence of any of the complex microbial agents used for fermentation in the folium artemisiae argyi extract of Comparative Examples 6-8 will lead to a significant reduction in the antioxidant effect of the composition.

[0048] 3. Safety test With the 2015 Cosmetic Safety Technical Specification as the reference standard, the compositions of Examples 1-5 were diluted with water to obtain samples in the form of 10% suspensions, and then the cosmetic irritation of the 5 groups of test samples was evaluated. The test method was a skin patch test, and 12 people aged 15-50 years old randomly distributed were tested. Test method: the test substance was placed in a patch tester, and the amount was 5 g. The patch tester with 5 groups of combined test substances was covered with a non-irritating cloth-based adhesive tape on the back of the test subject, and the skin surface was evenly attached by pressing with the palm for 24 hours. After 30 minutes of removing the test patch tester, the skin reaction was observed after the indentation disappeared. If the result is negative, the observation was performed again at 24 hours and 48 hours after the patch test.

[0049] Test result: the skin reactions of all the test subjects were negative. It is proved that the compositions of Examples 1-5 are mild and non-irritating to the skin, and are safe to use.

[0050] In summary, the penetration-enhancing composition of the present invention achieves efficient, safe, and long-lasting penetration effects through the synergistic effect of various components, and is suitable for the delivery of high-active ingredients in cosmetics.

[0051] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition having a penetration-promoting effect, characterized in that: The invention comprises the following components in parts by weight: 10-15 parts of ethoxydiglycol, 10-15 parts of dimethyl isosorbide, 5-10 parts of mugwort leaf extract and 1-6 parts of tea tree essential oil; wherein the preparation method of the mugwort leaf extract comprises the following steps: S1. Grind the wormwood leaves, soak them in deionized water, and extract them by water distillation to obtain wormwood oil; S2. Inoculating the composite fermentation bacterial liquid into a fermentation medium for fermentation to obtain a fermentation liquid; wherein the composite fermentation bacterial liquid comprises a spherical Lysinibacillus liquid, a Pseudomonas syringae liquid, and a Galacto-Saccharomyces-like bacterial liquid; S3. Adding the mugwort oil obtained in step S1 to the fermentation liquid obtained in step S2 to continue fermentation and cultivation, breaking the emulsion after obtaining the fermentation product, separating the water and oil phases, centrifuging, and taking the upper oil phase to obtain the mugwort extract.

2. The composition having penetration-promoting effect as claimed in claim 1, wherein In the preparation step S1 of the mugwort leaf extract, the amount of deionized water added is 8-10 times the total mass of the mugwort leaf, and the soaking time is 3-5 hours.

3. The composition with penetration-promoting effect as claimed in claim 1, wherein In the preparation step S2 of the mugwort extract, the inoculation amount of the composite fermentation bacteria liquid is 1-5% v / v, and the number of viable bacteria in the composite fermentation bacteria liquid is 3×10 9 -3×10 10 CFU / mL, the ratio of the number of live bacteria of spherical Lysinibacillus liquid, Pseudomonas syringae liquid and Galacto-Saccharomyces-like bacterial liquid in the composite fermentation bacterial liquid is (1-2): (1-3): 1, the fermentation temperature is 30-35°C, and the time is 15-18h.

4. The composition with penetration-enhancing effect as claimed in claim 1, wherein In the preparation step S3 of the mugwort extract, the volume ratio of the mugwort oil to the fermentation medium is (2-3):1, the temperature for continued fermentation culture is 30-35°C and the time is 18-24 hours, and the demulsification is performed by adding hydrochloric acid solution and sodium chloride to the fermentation product for demulsification, the mass concentration of the hydrochloric acid solution is 15-20%, and the mass ratio of the fermentation product, hydrochloric acid solution and sodium chloride is 1:(0.01-0.05):(0.1-0.3).

5. The composition with penetration-enhancing effect as claimed in claim 1, wherein The mass ratio of the mugwort leaf extract to the tea tree essential oil is (7-9): (2-4).

6. Use of the composition with penetration-enhancing efficacy according to any one of claims 1 to 5 in the preparation of cosmetics.

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