Flax olive oil for improving, preventing and treating cardiovascular diseases

Abstract

The invention discloses flax olive oil for improving, preventing and treating cardiovascular diseases, and belongs to the technical field of grease. The flax olive oil for improving, preventing and treating cardiovascular diseases mainly comprises the following raw material components: olive oil and flaxseed oil, and the mass ratio of the olive oil to the flaxseed oil is (1.5-2.5): 1. Wherein the flaxseed oil is obtained by screening flaxseeds, removing impurities, removing abnormal grains, crushing, extracting for multiple times by adopting a mixed extraction agent, degumming, decolorizing and deodorizing, the extraction temperature is 20-25 DEG C, the liquid-solid ratio is (9-10): 1mL / g, the single extraction time is 35-45 minutes, and the pressure of an extraction tank is 0.30-0.40 MPa; the mixed extraction agent is prepared from the following components in percentage by mass: 18.8 to 37.7 percent of ethanol, 29.7 to 30.4 percent of n-butane, 19.7 to 38.5 percent of dimethyl ether and 12.4 to 12.9 percent of water.

Description

TECHNICAL FIELD

[0001] The present application relates to a flax olive oil for improving the prevention and treatment of cardiovascular diseases. BACKGROUND

[0002] Cardiovascular diseases (CVD) are one of the leading causes of death worldwide, mainly including coronary heart disease, hypertension, stroke, etc. Its pathogenesis is complex, and is closely related to genetics, lifestyle, dietary structure and other factors. In recent years, with the improvement of people's living standards and the change of dietary structure, the incidence of metabolic diseases such as hyperlipidemia and atherosclerosis has been increasing year by year, further aggravating the burden of cardiovascular diseases.

[0003] In the process of preventing and treating cardiovascular diseases, dietary intervention is considered as one of the basic and effective strategies. As an important part of daily diet, the type and proportion of oil intake has a significant impact on cardiovascular health. There are many types of edible oils on the market, but many traditional vegetable oils have certain limitations in fatty acid composition, such as high content of saturated fatty acids, lack of ω-3 polyunsaturated fatty acids (PUFA) or poor stability, etc., which are difficult to fully meet the prevention and adjuvant therapy needs of cardiovascular diseases.

[0004] Flaxseed oil is rich in α-linolenic acid (ALA), which belongs to ω-3 series polyunsaturated fatty acids, and has many biological activities beneficial to cardiovascular health, such as anti-inflammatory, hypolipidemic, anti-arrhythmia, etc. However, due to its molecular structure containing multiple double bonds, it is easy to oxidize and deteriorate, which limits its application in high-temperature cooking. Olive oil is rich in monounsaturated fatty acids (mainly oleic acid) and various antioxidant components (such as polyphenols), which is widely considered to help reduce low-density lipoprotein cholesterol (LDL-C) and increase high-density lipoprotein cholesterol (HDL-C), thereby playing a protective role for cardiovascular. However, the content of ω-3 fatty acids in olive oil is relatively low, and the regulation effect on some cardiovascular risk factors is limited when used alone.

[0005] In order to solve the deficiencies of existing edible oil products in supporting cardiovascular health, it is urgent to provide a composite oil composition based on flaxseed oil and olive oil to achieve a more comprehensive effect of preventing and treating cardiovascular diseases. SUMMARY

[0006] The purpose of the present application is to provide a flax olive oil for improving the prevention and treatment of cardiovascular diseases, to solve the technical problems mentioned in the background.

[0007] The technical scheme to achieve the purpose of the present application is:

[0008] The application discloses a flax olive oil for improving prevention and treatment of cardiovascular diseases, and raw material components mainly include olive oil and flaxseed oil; the mass ratio of the olive oil and the flaxseed oil is 1.5-2.5:1.

[0009] Further, the flaxseed oil is obtained by screening, impurity removal and abnormal particle removal of flaxseed, crushing, screening, then adopting a mixed extractant to extract at least once, degumming, decolorization, deodorization, and then adding a decolorizing agent to the post-treatment extract; the post-treatment extract of the decolorizing agent is obtained by twice extraction of the decolorizing agent after decolorization.

[0010] Further, the screening step adopts a 30-40 mesh sieve.

[0011] Further, the mixed extractant includes 18.8-37.7% ethanol, 29.7-30.4% n-butane, 19.7-38.5% dimethyl ether and 12.4-12.9% water in terms of mass fraction.

[0012] Further, the extraction temperature of the mixed extractant is 20-25 DEG C, the liquid-solid ratio is 9-10:1 mL / g, the single extraction time is 35-45 min, and the extraction tank pressure is 0.30-0.40 MPa.

[0013] Further, the degumming process is as follows: the extracted flaxseed oil is heated to 30-50 DEG C, then a 50wt% citric acid solution is used to adjust the pH to 5.0, a mixed solution of phospholipase and water is added, wherein the enzyme amount is 60-100 mg / kg, the water amount is 2%, the enzyme is sufficiently hydrolyzed for 10-30 min, after the reaction is completed, centrifugation is carried out at a speed of at least 4500 r / min for 10 min, the upper oil phase is taken, and the degummed flaxseed oil is obtained.

[0014] Further, the decolorization process is as follows: the decolored flaxseed oil is preheated to 58-62 DEG C, then the decolorizing agent is added in an amount of 0.005-0.01 times the mass of the decolored flaxseed oil, and then stirred and mixed, then stirring decolorization is carried out at a decolorization temperature of 65-85 DEG C for 5-15 min, after the stirring is completed, centrifugation is carried out at a speed of at least 4500 r / min for 10 min, and the upper oil phase is taken, and the decolorized flaxseed oil is obtained.

[0015] Further, the mass ratio of the white clay and the activated carbon in the decolorizing agent is 5-10:1.

[0016] Further, the extraction step of the post-treatment extract of the decoloring agent is: mixing the decolored clay with the mixed solvent at a solid-liquid ratio of 1:10 g / mL, uniformly mixing, ultrasonic extraction for 20 min, vortex oscillation every 5 min during the ultrasonic extraction process, then centrifuging at a speed of at least 4500 r / min for 10 min, collecting the oil phase layer, repeating the extraction twice, combining the oil phase layers collected twice, then rotary evaporation at 40 DEG C until dryness, to obtain.

[0017] Further, the mixed solvent is obtained by mixing n-hexane and 95% ethanol at a mass ratio of 1:1.

[0018] By adopting the technical scheme, the present application has the following beneficial effects:

[0019] The improved flax olive oil for preventing and treating cardiovascular diseases of the present application is prepared by blending olive oil and flaxseed oil at a mass ratio of 1.5-2.5:1, so that the synergistic effect of the two in cardiovascular protection is fully exerted. The flaxseed oil is rich in alpha-linolenic acid and lignan, and the alpha-linolenic acid, as a precursor of omega-3 polyunsaturated fatty acid, can be converted into EPA and DHA in the body, and improve the hemorheological properties through the pathways of inhibiting platelet aggregation factor synthesis, reducing triglyceride level and regulating lipoprotein metabolism; the olive oil is centered on monounsaturated fatty acids such as oleic acid, and cooperates with polyphenols such as oleuropein and vitamin E, to effectively improve the vascular endothelial function by increasing the level of high-density lipoprotein and reducing the oxidative stress response of low-density lipoprotein, and the two form a unique fatty acid spectrum after compounding, which not only reduces the formation of arterial plaques through the mechanism of alpha-linolenic acid promoting reverse cholesterol transport, but also inhibits vascular inflammatory response by virtue of the antioxidant activity of olive polyphenols, and the lignan and oleic acid synergistically enhance the stability of cell membranes, so that the compound oil shows a multiplied effect in regulating blood pressure fluctuations and improving myocardial oxygen supply; the ratio design also takes into account the easy oxidation characteristics of the active ingredients of flaxseed oil, and uses the high stability of olive oil to extend the shelf life of the product, so that a compound functional oil with blood lipid regulation, anti-thrombosis and vascular protection functions is finally formed.

[0020] The linseed oil of the linseed oil of the application for preventing and treating cardiovascular diseases is obtained by screening, removing impurities and abnormal particles, crushing, screening, then extracting at least once by using a mixed extractant, degumming, decolorizing, deodorizing, and then adding a post-treatment extract of a decolorizing agent to the deodorized linseed oil; the post-treatment extract of the decolorizing agent is obtained by twice extracting the decolorizing agent after decolorization by using a mixed solvent; wherein the mixed extractant comprises 18.8-37.7% ethanol, 29.7-30.4% n-butane, 19.7-38.5% dimethyl ether, and 12.4-12.9% water by mass fraction, the extraction temperature is 20-25°C, the liquid-solid ratio is 9-10:1 mL / g, the single extraction time is 35-45 min, and the extraction tank pressure is 0.30-0.40 MPa; the oil extraction rate of the linseed is high, the cyclic peptide retention rate is high, and the cyanogenic glycoside removal rate of the linseed is high, and the defatted linseed meal obtained after extraction can be directly used for animal feed.

[0021] In the preparation of the linseed oil, the decolorizing agent after decolorization is twice extracted by using a mixed solvent, and the extracted extract is added to the deodorized linseed oil, so that the prepared linseed oil has a high cyclic peptide retention amount, wherein the cyclic peptide is also an important active ingredient in the linseed, the linseed cyclic peptide is a polypeptide formed by 8 to 10 hydrophobic amino acids connected by a peptide bond in a ring, and the molecular weight is about 1000 Da; the CLB in the linseed cyclic peptide can promote the generation of various anti-inflammatory metabolites such as 9-HOTrE, 9-HPOTrE, and 13-HOTrE by regulating ALA-related metabolic enzymes, and has a significant synergistic effect with ALA in anti-vascular inflammation in vivo and in vitro. DETAILED DESCRIPTION

[0022] In order to better understand the above technical solutions, the above technical solutions will be described in detail below in combination with specific embodiments.

[0023] Based on the embodiments in the application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the application.

[0024] The following examples are only used to more clearly illustrate the technical solutions of the application, and cannot be used to limit the protection scope of the application.

[0025] The linseed used is Ningya No. 11, purchased from Ningxia Yinchuan Fude Biological Food Engineering Co., Ltd., wherein the crude fat content is 36.82 wt%, the protein content is 23.27 wt%, the cyanide is 20.21 mg / kg, the moisture is 8.22 wt%, and the ash content is 3.49 wt%.

[0026] The phospholipase PLA1 is purchased from Novozymes Biological Technology Co., Ltd.

[0027] (Example 1)

[0028] A flax olive oil for improving the prevention and treatment of cardiovascular diseases, the raw material components include sunflower seed oil 69.8 parts, olive oil 15 parts, flaxseed oil 10 parts, safflower seed oil 2 parts, wheat germ oil 2 parts, peanut oil 1 part, walnut oil 0.1 part and camellia oil 0.1 part.

[0029] The flaxseed oil is obtained by screening, removing impurities and abnormal particles, crushing, passing through a 30-mesh sieve, then using a mixed extractant for 3 times of extraction, followed by vacuum desolventization, filtration, degumming, decolorization, deodorization, and then adding a decolorizing agent for post-treatment extraction; the post-treatment extract of the decolorizing agent is obtained by twice extraction of the decolorizing agent after decolorization using a mixed solvent; Table 1 below is the quality index of the flaxseed oil of Example 1:

[0030] Table 1

[0031] found Clarity (20°C) clear Odour, taste has the odour and taste inherent to linseed oil Colour light yellow Moisture and volatile matter content / % 0.195 Insoluble impurities content / % 0.040 Acid value (in KOH) (mg / g) 0.98

[0032] The quality indexes of the flaxseed oil obtained in Example 1 all meet the GB / T 8235-2019 standard, and can meet the index requirements of first-class finished flaxseed oil.

[0033] Among them, the flax peptide content of 100g of flaxseed oil of Example 1 is 645.35μg / g; the cyanogenic glycoside removal rate of flaxseed is 98.7%, and the oil extraction rate is 48.6%.

[0034] The mixed extractant includes 18.8% ethanol, 29.8% n-butane, 38.5% dimethyl ether and 12.9% water by mass fraction.

[0035] The extraction temperature using the mixed extractant is 20℃, the liquid-solid ratio is 10:1 mL / g, the single extraction time is 40min, and the extraction tank pressure is 0.357MPa.

[0036] The degumming process is as follows: the extracted flaxseed oil is heated to 30℃, then the pH is adjusted to 5.0 with a 50wt% citric acid solution, a mixed solution of phospholipase and water is added, wherein the enzyme amount is 100mg / kg, the water amount is 2%, the enzyme is fully hydrolyzed for 10min, after the reaction is completed, centrifugation is carried out at a speed of at least 4500r / min for 10min, the upper oil phase is taken, and the degummed flaxseed oil is obtained.

[0037] The decolorization process is as follows: the decolorized flaxseed oil is preheated to 58℃, then 0.005 times the mass of the decolorized flaxseed oil is added to the decoloring agent, and mixed uniformly, wherein the mass ratio of the white clay and activated carbon in the decoloring agent is 5:1, then the decoloring agent is stirred at a decoloring temperature of 85℃ for 10min, after stirring, centrifugation is performed at a speed of at least 4500r / min for 10min to obtain the upper oil phase, and the decolorized flaxseed oil is obtained.

[0038] The extraction step of the post-processing extract of the decoloring agent is as follows: the decolorized white clay is mixed with the mixed solvent at a solid-liquid ratio of 1:10g / mL, ultrasonic extraction is performed for 20min, vortex oscillation is performed every 5min during the ultrasonic extraction, then centrifugation is performed at a speed of at least 4500r / min for 10min, the oil phase layer is collected, the extraction is repeated twice, the oil phase layers collected in the two times are combined, then rotary evaporation is performed at 40℃ until dryness, and the post-processing extract of the decoloring agent is obtained.

[0039] The mixed solvent is obtained by mixing n-hexane and 95% ethanol at a mass ratio of 1:1.

[0040] The specific steps of the deodorization process are as follows: the decolorized flaxseed oil is added to the UIC-KDL1 molecular distillation instrument, the interfaces between the various vessels are connected, the vacuum pump is turned on to vacuum the system to 4.5*10 -2 mbar, the deodorization temperature is 230℃, the sample injection rate is about 2mL / min, the scraper rate is 150r / min, after deodorization is completed, the heavy phase is collected to obtain the deodorized flaxseed oil.

[0041] (Example 2)

[0042] A flax olive oil for improving the prevention and treatment of cardiovascular diseases, the raw material components include sunflower seed oil 48.6 parts, olive oil 28 parts, flaxseed oil 14 parts, safflower seed oil 3 parts, wheat germ oil 4 parts, peanut oil 2 parts, walnut oil 0.2 parts and camellia oil 0.2 parts.

[0043] The flaxseed oil is obtained by screening, removing impurities and abnormal particles, crushing, passing through a 30-mesh sieve, then performing 3 times of extraction with a mixed extractant, followed by vacuum desolventization, filtration, degumming, decolorization, deodorization, and then adding the post-processing extract of the decoloring agent; the post-processing extract of the decoloring agent is obtained by twice extraction of the decoloring agent with a mixed solvent after decolorization, and Table 2 below shows the quality indicators of the flaxseed oil of Example 2:

[0044] Table 2

[0045] found Clarity (20°C) clear Odour, taste has the odour and taste inherent to linseed oil Colour light yellow Moisture and volatile matter content / % 0.190 Insoluble impurities content / % 0.028 Acid value (in KOH) (mg / g) 0.75

[0046] The quality indicators of the flaxseed oil obtained in Example 2 all meet the GB / T 8235-2019 standard, and can meet the requirements of the first-grade finished flaxseed oil.

[0047] The linseed oil of Example 2 has a linseed cyclolinopeptide content of 656.65 μg / g per 100 g, a cyanogenic glycoside removal rate of 99.1%, and an oil extraction rate of 49.2%.

[0048] The mixed extractant comprises 28.2% ethanol, 30.4% n-butane, 29.0% dimethyl ether, and 12.4% water by mass fraction.

[0049] The extraction temperature is 20°C, the liquid-solid ratio is 10:1 mL / g, the single extraction time is 40 min, and the extraction tank pressure is 0.324 MPa.

[0050] The degumming process is as follows: the extracted linseed oil is heated to 50°C, then a 50wt% citric acid solution is used to adjust the pH to 5.0, a mixed solution of phospholipase and water is added, the amount of enzyme added is 60 mg / kg, and the amount of water added is 2%, the enzyme is fully hydrolyzed for 30 min, and after the reaction is completed, centrifugation is performed at a speed of at least 4500 r / min for 10 min, the upper oil phase is taken, and the degummed linseed oil is obtained.

[0051] The bleaching process is as follows: the bleached linseed oil is preheated to 60°C, then 0.01 times the mass of the bleached linseed oil of bleaching agent is added and mixed, wherein the mass ratio of the bleaching agent to white clay and activated carbon is 10:1, the bleaching agent is then stirred and bleached at a bleaching temperature of 75°C for 15 min, after the stirring is completed, centrifugation is performed at a speed of at least 4500 r / min for 10 min, and the upper oil phase is taken to obtain the bleached linseed oil.

[0052] The extraction step of the post-treatment extract of the bleaching agent is as follows: the bleached white clay is mixed with the mixed solvent at a solid-liquid ratio of 1:10 g / mL, ultrasonic extraction is performed for 20 min, vortex oscillation is performed every 5 min during the ultrasonic extraction, then centrifugation is performed at a speed of at least 4500 r / min for 10 min, the oil phase layer is collected, the extraction is repeated twice, the oil phase layers collected in the two times are combined, then rotary evaporation is performed at 40°C until dryness, and the post-treatment extract of the bleaching agent is obtained.

[0053] The mixed solvent is obtained by mixing n-hexane and 95% ethanol at a mass ratio of 1:1.

[0054] The specific steps of the deodorization process are as follows: the bleached linseed oil is added to a UIC-KDL1 molecular distillation instrument, the interfaces between the various vessels are connected, a vacuum pump is turned on to vacuum the system to 4.5*10 -2 mbar, the deodorization temperature is 230°C, the sample injection rate is about 2 mL / min, the scraper rate is 150 r / min, after the deodorization is completed, the heavy phase is collected to obtain the deodorized linseed oil.

[0055] (Example 3)

[0056] A flax olive oil for improving the prevention and treatment of cardiovascular diseases, the raw material components include sunflower seed oil 31.4 parts, olive oil 40 parts, flaxseed oil 16 parts, safflower seed oil 4 parts, wheat germ oil 5 parts, peanut oil 3 parts, walnut oil 0.3 parts and camellia oil 0.3 parts.

[0057] The flaxseed oil is obtained by screening, removing impurities and abnormal particles, crushing, passing through a 30-mesh sieve, then using a mixed extractant for 3 times of extraction, followed by vacuum desolventization, filtration, degumming, decolorization, deodorization, and then adding a decolorizing agent for post-treatment extraction; the post-treatment extraction of the decolorizing agent is obtained by using a mixed solvent for twice extraction after decolorization, and Table 3 below is the quality index of the flaxseed oil of Example 3:

[0058] Table 3

[0059]

[0060]

[0061] The quality indexes of the flaxseed oil obtained in Example 3 all meet the GB / T 8235-2019 standard, and can meet the index requirements of first-class finished flaxseed oil.

[0062] Among them, the flax peptide content of 100g of flaxseed oil of Example 3 is 649.12μg / g, the cyanogenic glycoside removal rate of flaxseed is 98.9%, and the oil extraction rate is 46.8%.

[0063] The mixed extractant includes 37.7% ethanol, 29.7% n-butane, 19.7% dimethyl ether and 12.9% water by mass fraction.

[0064] The extraction temperature of the mixed extractant is 20℃, the liquid-solid ratio is 10:1 mL / g, the single extraction time is 40min, and the extraction tank pressure is 0.288MPa.

[0065] The degumming process is as follows: the extracted flaxseed oil is heated to 40℃, then the pH is adjusted to 5.0 with a 50wt% citric acid solution, a mixed solution of phospholipase and water is added, wherein the enzyme amount is 80mg / kg, the water amount is 2%, the enzyme is fully hydrolyzed for 20min, after the reaction is completed, centrifugation is carried out at a speed of at least 4500r / min for 10min, the upper oil phase is taken, and the degummed flaxseed oil is obtained.

[0066] The decolorization process is as follows: the decolorized flaxseed oil is preheated to 62℃, then 0.01 times the mass of the decolorized flaxseed oil of decolorizing agent is added and mixed, wherein the mass ratio of white clay and activated carbon in the decolorizing agent is 10:1, then the decolorizing agent is stirred at a decolorization temperature of 85℃ for 5min, after stirring, the upper oil phase is taken by centrifugation at a speed of at least 4500r / min for 10min, and the decolorized flaxseed oil is obtained.

[0067] The extraction step of the post-treatment extract of the decolorizing agent is as follows: the decolorized white clay is mixed with the mixed solvent at a solid-liquid ratio of 1:10g / mL, ultrasonic extraction is performed for 20min, vortex oscillation is performed every 5min during the ultrasonic extraction, then centrifugation is performed at a speed of at least 4500r / min for 10min, the oil phase layer is collected, the extraction is repeated twice, the oil phase layers collected twice are combined, then rotary evaporation is performed at 40℃ until dryness, and the post-treatment extract of the decolorizing agent is obtained.

[0068] The mixed solvent is obtained by mixing n-hexane and 95% ethanol at a mass ratio of 1:1.

[0069] The specific steps of the deodorization process are as follows: the decolorized flaxseed oil is added into a UIC-KDL1 molecular distillation instrument, the interfaces between the vessels are connected, the vacuum pump is turned on to vacuum the system to 4.5*10 -2 mbar, the deodorization temperature is 230℃, the sample feeding rate is about 2mL / min, the scraper rate is 150r / min, after deodorization is completed, the heavy phase is collected to obtain the deodorized flaxseed oil.

[0070] (Comparative Example 1)

[0071] The difference between Comparative Example 1 and Example 2 lies in the extraction step, wherein the mixed extractant includes 80wt% ethanol solution, n-hexane, and sodium hydroxide; wherein the material-alcohol ratio is 1:3 (w / v), the material-alkane ratio is 1:4 (w / v), the mass-volume ratio of sodium hydroxide to ethanol solution is 0.001:1, the extraction temperature is 50℃, the extraction time is 1h, the extraction pressure is atmospheric pressure, and the extraction times is 1; the remaining components and temperatures are the same as those of Example 1.

[0072] Table 4 below is the quality index of the flaxseed oil of Comparative Example 1:

[0073] Table 4

[0074] found Clarity (20°C) clear Odour, taste has the odour and taste inherent to linseed oil Colour light yellow Moisture and volatile matter content / % 0.198 Insoluble impurities content / % 0.045 Acid value (in KOH) (mg / g) 0.86

[0075] The quality index of the flaxseed oil obtained by Comparative Example 1 meets the GB / T 8235-2019 standard, and can meet the index requirements of first-class finished flaxseed oil.

[0076] The linolenic acid content of 100 g of the flaxseed oil of Comparative Example 1 is 584.62 μg / g, the cyanogenic glycoside removal rate of the flaxseed is 98.5%, and the oil extraction rate is 43.7%.

[0077] In summary, the linolenic acid content of the flaxseed oil obtained in Examples 1 to 3 is retained more, the cyanogenic glycoside removal is better, and the oil extraction rate is higher.

[0078] Healthy SD male rats weighing 200±20 g were selected, and the maintenance feed was composed of corn, bean cake, fish meal and other raw materials. The high-fat feed was prepared by adding 15% of lard to the maintenance feed, and the flaxseed olive oil feed was prepared by adding 15% of the flaxseed olive oil obtained in Examples 1 to 3 to the maintenance feed. The temperature in the feeding room was maintained at 22±2°C, the humidity was maintained at 55±5%, and the darkness and light were adjusted at intervals of 12 hours.

[0079] After normal feeding with the maintenance feed for one week, 10 rats were randomly selected as the normal control group and continued to be fed with the maintenance feed. The remaining rats were fed with the high-fat feed as the high-fat diet group. After 30 days of feeding, they were randomly divided into 4 groups, 10 rats in each group. One group was the model group and continued to be fed with the high-fat feed, and the remaining 3 groups were fed with the flaxseed olive oil feed containing the flaxseed olive oil obtained in Examples 1 to 3. After 4 weeks of feeding, the systolic blood pressure of rats in each group was measured every week.

[0080] Table 5 below is the systolic blood pressure of rats in the control group and the groups of Examples 1 to 3 every week:

[0081] Table 5

[0082]

[0083]

[0084] The same row in the table with different lowercase letters a to d indicates that the statistical difference is significant (p<0.05).

[0085] As can be seen from the above table, the flaxseed olive oil of Examples 1 to 3 can significantly reduce the blood pressure of hypertensive rats and has obvious antihypertensive function.

[0086] The above specific examples further illustrate the purpose, technical solutions and advantages of the present application. It should be understood that the above description is only a specific embodiment of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A flaxseed olive oil for improving and preventing cardiovascular diseases, characterized in that: The raw material components mainly include olive oil and linseed oil; the mass ratio of the olive oil to the linseed oil is 1.5-2.5:

1.

2. The flaxseed olive oil for improving and preventing cardiovascular diseases according to claim 1, characterized in that The linseed oil is obtained by screening, removing impurities and abnormal particles from linseeds, then crushing and sieving, and then extracting at least once with a mixed extractant, followed by degumming, decoloring, deodorizing, and adding a decolorant to obtain a post-processed extract; the post-processed extract of the decolorant is obtained by extracting the decolorized decolorant twice with a mixed solvent.

3. The flaxseed olive oil for improving and preventing cardiovascular diseases according to claim 2, characterized in that: A 30-40 mesh sieve is used in the sieving step.

4. The flaxseed olive oil for improving and preventing cardiovascular diseases according to claim 2, characterized in that The mixed extractant comprises, by mass fraction, 18.8-37.7% ethanol, 29.7-30.4% n-butane, 19.7-38.5% dimethyl ether and 12.4-12.9% water.

5. The flaxseed olive oil for improving and preventing cardiovascular diseases according to claim 4, characterized in that: The extraction temperature of the mixed extractant is 20-25° C., the liquid-solid ratio is 9-10:1 mL / g, the single extraction time is 35-45 min, and the extraction tank pressure is 0.30-0.40 MPa.

6. The flaxseed olive oil for improving and preventing cardiovascular diseases according to claim 2, characterized in that: The degumming process is as follows: heating the extracted linseed oil to 30-50° C., then adjusting the pH to 5.0 with a 50wt% citric acid solution, adding a mixed solution of phosphatase and water, wherein the enzyme amount is 60-100 mg / kg, the water amount is 2%, and fully enzymolyzing for 10-30 minutes. After the reaction is completed, centrifuging at a speed of at least 4500 r / min for 10 minutes, and collecting the upper oil phase to obtain the degummed linseed oil.

7. The flaxseed olive oil for improving and preventing cardiovascular diseases according to claim 2, characterized in that: The decolorization process is as follows: preheating the decolorized linseed oil to 58-62° C., then adding a decolorizer in an amount of 0.005-0.01 times the mass of the decolorized linseed oil and mixing the mixture, then stirring and decolorizing the mixture at a decolorization temperature of 65-85° C. for 5-15 minutes, and after stirring, centrifuging the mixture at a speed of at least 4500 r / min for 10 minutes to obtain an upper oil phase to obtain the decolorized linseed oil.

8. The flaxseed olive oil for improving and preventing cardiovascular diseases according to claim 7, characterized in that: The mass ratio of white clay to activated carbon in the decolorizer is 5 to 10:

1.

9. The flaxseed olive oil for improving and preventing cardiovascular diseases according to claim 8, characterized in that: The extraction steps of the post-processed extract of the decolorizing agent are as follows: the decolorized white clay and the mixed solvent are evenly mixed at a solid-liquid ratio of 1:10 g / mL, ultrasonic extraction is performed for 20 minutes, vortex oscillation is performed every 5 minutes during the ultrasonic extraction process, and then centrifugation is performed at a speed of at least 4500 r / min for 10 minutes to collect the oil phase layer, the extraction is repeated twice, the oil phase layers collected twice are combined, and then rotary evaporation at 40°C to dryness is obtained.

10. The flaxseed olive oil for improving and preventing cardiovascular diseases according to claim 8, characterized in that: The mixed solvent is obtained by mixing n-hexane and 95% ethanol in a mass ratio of 1:1.

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