Micro-ecological fermentation and traditional Chinese medicine formula technology for treating poultry egg-borne bacterial diseases

Abstract

The invention provides a micro-ecological fermentation and traditional Chinese medicine formula technology for treating poultry egg-borne bacterial diseases. The traditional Chinese medicine microbial fermentation agent is prepared from the following components: 15 to 20 parts of herba portulacae, 11 to 21 parts of fructus forsythiae, 14 to 17 parts of cortex phellodendri, 10 to 15 parts of herba artemisiae annuae, 5 to 10 parts of fructus quisqualis, 5 to 8 parts of radix dichroae, 5 to 10 parts of radix curcumae, 5 to 10 parts of fructus mume, 5 to 12 parts of flos lonicerae, 4 to 8 parts of radix ophiopogonis, 1 to 3 parts of bacillus subtilis, 2 to 5 parts of lactobacillus plantarum, 3 to 5 parts of lactobacillus acidophilus and 2 to 4 parts of lactic acid bacteria. The traditional Chinese medicine composition has obvious inhibition effects on escherichia coli and staphylococcus aureus in the poultry egg-borne bacterial diseases, has better effects than single use of bacteria and traditional Chinese medicines, effectively treats the poultry egg-borne bacterial diseases caused by various reasons, and can well improve the immunity of the organism and increase the survival rate of the poultry eggs.

Description

Technical Field

[0001] The invention relates to the technical field of poultry Chinese medicinal microecological preparations, in particular to a microecological fermentation and Chinese medicinal formula preparation process for treating egg-transmitted bacterial diseases of breeding poultry. Background Art

[0002] Egg hygiene: The microorganisms in fresh eggs may come from the ovaries, reproductive cavity, or from unclean egg-laying sites and transportation and sales links. Microorganisms can enter the egg through the eggshell and grow and reproduce, causing corruption and deterioration. Fresh eggs must be stored at low temperatures, and the types of contaminated microorganisms and the severity of contamination vary during the formation process, in the circulation field, or in the egg processing factory due to the different external environments in which they are located. Microorganisms may contaminate eggs during the formation process and the storage process. The microorganisms found in eggs are mainly bacteria and molds, and most of them are aerobic bacteria, but there are also anaerobic bacteria. The bacteria found in eggs are mainly Staphylococcus, Streptococcus, Escherichia coli, Proteobacterium, Pseudomonas, Salmonella, etc.; the molds are mainly Aspergillus, Penicillium, Mucor, Geotrichum and White Mold, etc.

[0003] In the past, the main method to solve problems such as decreased egg production and increased disease incidence was to add antibiotics to feed and drinking water. However, long-term and high-dose additions have led to many problems, such as the development of drug-resistant strains and excessive drug residues in eggs, causing serious environmental pollution and threatening human health. Using Chinese herbal medicine instead of antibiotics can effectively reduce drug residue problems in animal husbandry.

[0004] However, due to the limitations of the digestive system of laying hens, traditional Chinese herbal preparations have problems such as slow effect, low utilization rate, and unclear medicinal effect. The emergence of bio-fermentation Chinese medicine technology has greatly improved the efficacy of Chinese herbal medicines, and has gradually shown more obvious effects in preventing diseases and improving production performance. Summary of the Invention

[0005] (1) Technical problems solved

[0006] In view of the shortcomings of the existing technology, the present invention provides a microecological fermentation and Chinese medicine formulation process for treating egg-transmitted bacterial diseases of breeding poultry, which solves the problems of slow effect, low utilization rate and unclear medicinal effect of traditional Chinese herbal medicine preparations.

[0007] (2) Technical solution

[0008] To achieve the above objectives, the present invention is implemented through the following technical solutions: a microecological fermentation and traditional Chinese medicine formula process for treating egg-transmitted bacterial diseases of poultry, which is composed of the following components in parts by weight:

[0009] 15-20 parts of Portulaca Oleracea, 11-21 parts of Fructus Forsythiae, 14-17 parts of Cortex Phellodendri, 10-15 parts of Artemisia Annua, 5-10 parts of Fructus Quisqualis, 5-8 parts of Radix Dichroae, 5-10 parts of Curcuma Radix, 5-10 parts of Prunus Mume, 5-12 parts of Flos Lonicerae, 4-8 parts of Radix Ophiopogonis, 1-3 parts of Bacillus subtilis, 2-5 parts of Lactobacillus plantarum, 3-5 parts of Lactobacillus acidophilus, and 2-4 parts of lactic acid bacteria.

[0010] Preferably, 0.6×10 RCFU of bacteria is used for fermentation per 1g of the Chinese medicine.

[0011] Preferably, the viable count of the Bacillus subtilis, Lactobacillus plantarum, Lactobacillus acidophilus and lactic acid bacteria is ≥10 billion CFU / g.

[0012] The Chinese medicine formulation according to any one of claims 1 to 3 is prepared by the following method:

[0013] S1. Feeding purslane, forsythia, phellodendron, artemisia annua, quisqualis, radix ferox, radix changshanis, turmeric, phellodendron, plum, honeysuckle, and ophiopogon into a grinding device for grinding, and mixing after grinding to obtain a preliminary mixture;

[0014] S2. Add Bacillus subtilis, Lactobacillus plantarum, and Lactobacillus acidophilus to the preliminary mixture, spray in a wetting equivalent of water, mix well, maintain at 33-37° C. for 17-24 hours, then dry and mix well to obtain a Chinese medicine compound preparation.

[0015] Preferably, the drying temperature is 40-65° C., and the drying is performed until the moisture content is less than 12 wt %.

[0016] Preferably, the preparation is an oral solution, tablet, pill, granule or capsule;

[0017] Usage: Mix with water, add 50kg of water for every 100g, and use for 4-7 days.

[0018] (3) Beneficial effects

[0019] The present invention provides a microecological fermentation and traditional Chinese medicine formula process for treating egg-transmitted bacterial diseases of breeding poultry, which has the following beneficial effects:

[0020] 1. The Chinese medicinal microbial fermentation agent of the present invention has a significant inhibitory effect on Escherichia coli and Staphylococcus aureus in poultry egg-transmitted bacterial diseases, and the effect is better than that of using bacteria and Chinese medicine alone. It can effectively treat poultry egg-transmitted bacterial diseases caused by various reasons, can well improve the body's immunity, and increase the survival rate of its poultry eggs. DETAILED DESCRIPTION

[0021] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] Example 1:

[0023] The present invention provides a microecological fermentation and traditional Chinese medicine formulation process for treating egg-transmitted bacterial diseases of poultry, which is composed of the following components in parts by weight:

[0024] 15 parts of Herbs of Portulaca Oleracea, 11 parts of Fructus Forsythiae, 14 parts of Cortex Phellodendri, 10 parts of Artemisia Annua, 5 parts of Fructus Quisqualis, 5 parts of Radix Dichroae, 5 parts of Radix Curcumae, 5 parts of Prunus Mume, 5 parts of Flos Lonicerae, 4 parts of Radix Ophiopogonis, 1 part of Bacillus subtilis, 2 parts of Lactobacillus plantarum, 3 parts of Lactobacillus acidophilus, and 2 parts of lactic acid bacteria.

[0025] For every 1g of Chinese medicine, 0.6×10RCFU of bacteria was used for fermentation.

[0026] The viable count of Bacillus subtilis, Lactobacillus plantarum, Lactobacillus acidophilus and lactic acid bacteria is ≥10 billion CFU / g.

[0027] The Chinese medicine formulation according to any one of claims 1 to 3 is prepared by the following method:

[0028] S1. Feeding purslane, forsythia, phellodendron, artemisia annua, quisqualis, radix ferox, radix changshanis, turmeric, phellodendron, plum, honeysuckle, and ophiopogon into a grinding device for grinding, and mixing after grinding to obtain a preliminary mixture;

[0029] S2. Add Bacillus subtilis, Lactobacillus plantarum, and Lactobacillus acidophilus to the preliminary mixture, spray in a wetting equivalent of water, mix well, maintain at 33-37° C. for 17-24 hours, then dry and mix well to obtain a Chinese medicine compound preparation.

[0030] The drying temperature is 40-65° C., and the drying temperature is until the moisture content is less than 12 wt %.

[0031] The preparations are oral solution, tablets, pills, granules or capsules.

[0032] The Chinese medicinal ingredients and strains in this microbial fermentation agent have the following effects:

[0033] Purslane: Purslane has a sour, cold, and lubricating nature, and its texture is smooth and astringent. It enters the large intestine and liver meridians. It clears heat and toxins, cools the blood, and eliminates heat and toxins from within. It also lubricates the intestines, promoting the excretion of damp heat or heat toxins through the stool. It also relieves damp heat and relieves stranguria, guiding heat and toxins out through the urine. It is effective in treating heat dysentery and bloody dysentery, as well as blood-heat bleeding and painful stranguria. It can be used as both a medicine and a food, is mild in taste, and is easy to eat. It can also be used as a weight loss health food.

[0034] Forsythia: Forsythia is bitter and dispersing, slightly cold and clearing, and light in nature, floating upward, entering the lung, heart, and small intestine meridians. It clears heat and toxins, dispels wind-heat, and also disperses stagnation, promotes urination, and reduces swelling. It is a strong medicinal herb, primarily clearing the body, while also penetrating the middle, dispersing stagnation and promoting urination. It is suitable for treating heat toxins, wind-heat, damp-heat, and swelling. It is known as a panacea for sores.

[0035] Huang Bai: Huang Bai is bitter and cold-clearing, dry and sinking, and enters the kidney and bladder meridians. It not only clears away excess heat (fire) and resolves heat toxins, but also dries out dampness, removes dampness toxins, and resolves damp-heat, clearing kidney fire (Xiang Huo) and relieving deficiency heat. It is a key herb for treating damp-heat and fire toxins and is widely used for symptoms of damp-heat and fire toxins. Compared with Coptis chinensis, its heat-clearing and damp-damp-removing properties are weaker, and its effects are more focused on the kidneys and lower bladder. It is best at clearing Xiang Huo, relieving deficiency heat, and removing damp-heat in the lower bladder. It combines the functions of clearing excess fire, damp-heat, and relieving deficiency heat, and is suitable for treating excess heat and fire toxins, damp-heat, and deficiency heat.

[0036] Artemisia annua: Artemisia annua is bitter and cold, clearing and dispersing, and pungent and fragrant, penetrating and dispersing. It enters the liver and gallbladder meridians. It has both clearing and penetrating properties, with clearing as the main feature, and penetrating within. It can relieve both deficiency-heat and excess-heat, cooling blood-heat; it can clear summer-heat and purge liver and gallbladder heat; it can eliminate malaria-induced fever and purge nutrient-heat; it can purge latent heat in the yin and relieve superficial heat, clearing both deficiency-heat and excess-heat, and can also be used to treat both superficial and superficial heat.

[0037] Quisqualis chinensis: Quisqualis chinensis has a sweet and warming nature. It enters the spleen and stomach meridians. This product mainly contains organic acids, fatty acids, alkaloids, and amino acids, which have the effect of killing insects and eliminating accumulation. It is used to treat ascariasis, pinworms, abdominal pain caused by worm accumulation, and malnutrition in children.

[0038] Changshan: Changshan is bitter and cold in nature. It mainly enters the lung, liver, and heart meridians. It usually has the effects of vomiting phlegm and stopping malaria. It is mainly used to treat symptoms such as phlegm and fluid accumulation, chest and diaphragm congestion, and malaria.

[0039] Curcuma: Curcuma can usually promote blood circulation and relieve pain, and it can also relieve Qi stagnation and blood stasis. It also has the effect of clearing the heart and cooling the blood.

[0040] Black plum: Black plum is cultivated in various parts of my country, with the largest amount in the provinces south of the Yangtze River. It contains citric acid, malic acid, succinic acid, sugars, sitosterol, vitamin C and other ingredients, and has the effects of relieving vomiting, astringing the intestines and stopping diarrhea, and relieving cough and thirst.

[0041] Honeysuckle: Honeysuckle is sweet, cold, and clearing, with a light, soothing, and dispersing effect. It enters the lung, stomach, and large intestine meridians. It is good at clearing away heat and toxicity, as well as dispersing wind-heat. It is a strong, yet not bitter, herbal remedy for dispersing heat and toxicity. It is also easy to take, with a clearing effect, and is suitable for all cases of heat and toxicity.

[0042] Ophiopogon japonicus: Ophiopogon japonicus has a moist and sweet tonic effect, a slightly bitter and cold clearing effect, and enters the lung, heart, and stomach meridians. It is a nourishing and refreshing herb. It nourishes yin and produces fluid, moistening the lungs and benefiting the stomach. It also nourishes the mind and relieves restlessness and calms the nerves. It also moisturizes the intestines and promotes bowel movements, treating lung and stomach yin deficiency, restlessness and insomnia, and constipation caused by dry intestines.

[0043] Bacillus subtilis: Bacillus subtilis has colonization, exclusivity and reproduction properties, which can significantly limit the contact, adhesion or colonization of pathogenic intestinal microorganisms with the intestinal mucosal epithelium; it can stimulate the host's immune response, enhance humoral immunity and cellular immunity, and can also enhance the phagocytic activity of macrophages, as well as the complement and reticuloendothelial system functions;

[0044] Lactobacillus plantarum: An anaerobic bacterium, it produces large amounts of organic acids such as acetic acid and propionic acid, as well as digestive enzymes, during its metabolism. These can lower the pH of the gastrointestinal tract, help activate and increase the activity of pepsin, and effectively inhibit the growth and reproduction of pathogens in an acidic environment. The lactobacilli in its metabolites have inhibitory or killing effects on Escherichia coli, Salmonella, and other bacteria. It also improves the intestinal microenvironment, stimulates the development of intestinal immune tissue, and enhances intestinal immune function.

[0045] Lactobacillus acidophilus: Lactobacillus acidophilus refers to Lactobacillus acidophilus, which is a common probiotic in the gastrointestinal tract. It belongs to the genus Lactobacillus in the family Lactobacillaceae. It is a Gram-positive bacterium with a slender rod-shaped shape. It has the functions of promoting protein digestion and absorption, nourishing the body, improving anti-infection ability, and regulating intestinal flora.

[0046] Lactic acid bacteria: Lactic acid bacteria increase lactose digestion and stimulate intestinal mucosal lactase activity to treat osmotic diarrhea; modify toxin receptors by producing certain enzymes, blocking or reducing the binding of toxins to intestinal mucosal receptors; produce organic acids, free fatty acids, ammonia, hydrogen peroxide and bacteriocins, which have antibacterial effects.

[0047] Example 2:

[0048] The present invention provides a microecological fermentation and traditional Chinese medicine formulation process for treating egg-transmitted bacterial diseases of poultry, which is composed of the following components in parts by weight:

[0049] 16 parts of Portulaca Oleracea, 14 parts of Fructus Forsythiae, 15 parts of Cortex Phellodendri, 13 parts of Artemisia Annua, 7 parts of Fructus Quisqualis, 6 parts of Radix Dichroae, 7 parts of Radix Curcumae, 8 parts of Prunus Mume, 9 parts of Flos Lonicerae, 6 parts of Radix Ophiopogonis, 2 parts of Bacillus subtilis, 4 parts of Lactobacillus plantarum, 4 parts of Lactobacillus acidophilus, and 3 parts of lactic acid bacteria.

[0050] For every 1g of Chinese medicine, 0.6×10RCFU of bacteria was used for fermentation.

[0051] The viable count of Bacillus subtilis, Lactobacillus plantarum, Lactobacillus acidophilus and lactic acid bacteria is ≥10 billion CFU / g.

[0052] The Chinese medicine formulation according to any one of claims 1 to 3 is prepared by the following method:

[0053] S1. Feeding purslane, forsythia, phellodendron, artemisia annua, quisqualis, radix ferox, radix changshanis, turmeric, phellodendron, plum, honeysuckle, and ophiopogon into a grinding device for grinding, and mixing after grinding to obtain a preliminary mixture;

[0054] S2. Add Bacillus subtilis, Lactobacillus plantarum, and Lactobacillus acidophilus to the preliminary mixture, spray in a wetting equivalent of water, mix well, maintain at 33-37° C. for 17-24 hours, then dry and mix well to obtain a Chinese medicine compound preparation.

[0055] The drying temperature is 40-65° C., and the drying temperature is until the moisture content is less than 12 wt %.

[0056] The preparations are oral solution, tablets, pills, granules or capsules.

[0057] Example 3:

[0058] The present invention provides a microecological fermentation and traditional Chinese medicine formulation process for treating egg-transmitted bacterial diseases of poultry, which is composed of the following components in parts by weight:

[0059] 20 parts of Herbs of Portulaca Oleracea, 21 parts of Fructus Forsythiae, 17 parts of Cortex Phellodendri, 15 parts of Artemisia Annua, 10 parts of Fructus Quisqualis, 8 parts of Radix Dichroae, 10 parts of Radix Curcumae, 10 parts of Prunus Mume, 12 parts of Flos Lonicerae, 8 parts of Radix Ophiopogonis, 3 parts of Bacillus subtilis, 5 parts of Lactobacillus plantarum, 5 parts of Lactobacillus acidophilus, and 4 parts of lactic acid bacteria.

[0060] For every 1g of Chinese medicine, 0.6×10RCFU of bacteria was used for fermentation.

[0061] The viable count of Bacillus subtilis, Lactobacillus plantarum, Lactobacillus acidophilus and lactic acid bacteria is ≥10 billion CFU / g.

[0062] The Chinese medicine formulation according to any one of claims 1 to 3 is prepared by the following method:

[0063] S1. Feeding purslane, forsythia, phellodendron, artemisia annua, quisqualis, radix ferox, radix changshanis, turmeric, phellodendron, plum, honeysuckle, and ophiopogon into a grinding device for grinding, and mixing after grinding to obtain a preliminary mixture;

[0064] S2. Add Bacillus subtilis, Lactobacillus plantarum, and Lactobacillus acidophilus to the preliminary mixture, spray in a wetting equivalent of water, mix well, maintain at 33-37° C. for 17-24 hours, then dry and mix well to obtain a Chinese medicine compound preparation.

[0065] The drying temperature is 40-65° C., and the drying temperature is until the moisture content is less than 12 wt %.

[0066] The preparations are oral solution, tablets, pills, granules or capsules.

[0067] Example 4:

[0068] 400 laying hens aged 60 weeks were randomly divided into 4 groups, with 100 hens in each group. Groups 1-3 were fed with feed containing the Chinese medicinal proecological preparations of Examples 1-3 in sequence according to the methods of Examples 1-3, and Group 4 was fed with normal commercial feed in the same manner as Groups 1-3. The laying performance of the laying hens was recorded after 1 week, and the survival rate of the laying hens was recorded subsequently. The actual results are shown in Table 1:

[0069] Group Number of experiments / Egg production rate / % Survival rate / % Example 1 100 80.31 85 Example 2 100 86.22 94 Example 3 100 82.65 82 Comparative Example 100 77.47 69

[0070] Therefore, it can be clearly seen that the Chinese medicine formula preparation provided by the present invention can not only improve the egg production rate of chickens, but also the survival rate can reach more than 80%, can treat and prevent egg-transmitted bacterial diseases of breeding poultry, and increase the survival rate of its breeding poultry eggs.

[0071] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A microecological fermentation and Chinese medicine formula process for treating egg-transmitted bacterial diseases of poultry, characterized in that: In parts by weight, it is composed of the following components: 15-20 parts of Portulaca Oleracea, 11-21 parts of Fructus Forsythiae, 14-17 parts of Cortex Phellodendri, 10-15 parts of Artemisia Annua, 5-10 parts of Fructus Quisqualis, 5-8 parts of Radix Dichroae, 5-10 parts of Curcuma Radix, 5-10 parts of Prunus Mume, 5-12 parts of Flos Lonicerae, 4-8 parts of Radix Ophiopogonis, 1-3 parts of Bacillus subtilis, 2-5 parts of Lactobacillus plantarum, 3-5 parts of Lactobacillus acidophilus, and 2-4 parts of lactic acid bacteria.

2. A microecological fermentation and Chinese medicine formula process for treating egg-transmitted bacterial diseases of poultry according to claim 1, characterized in that: Every 1g of the Chinese medicine is fermented with 0.6×10 RCFU of bacteria.

3. A microecological fermentation and Chinese medicine formula process for treating egg-transmitted bacterial diseases of poultry according to claim 1, characterized in that: The viable count of the Bacillus subtilis, Lactobacillus plantarum, Lactobacillus acidophilus and lactic acid bacteria is ≥10 billion CFU / g.

4. The Chinese medicine formulation according to any one of claims 1 to 3, characterized in that: It is prepared by the following method: S1. Feeding purslane, forsythia, phellodendron, artemisia annua, quisqualis, radix ferox, radix changshanis, turmeric, phellodendron, plum, honeysuckle, and ophiopogon into a grinding device for grinding, and mixing after grinding to obtain a preliminary mixture; S2. Add Bacillus subtilis, Lactobacillus plantarum, and Lactobacillus acidophilus to the preliminary mixture, spray in a wetting equivalent of water, mix well, maintain at 33-37° C. for 17-24 hours, then dry and mix well to obtain a Chinese medicine compound preparation.

5. The Chinese medicine formula process according to any one of claims 1 to 4, characterized in that: The drying temperature is 40-65° C., and the drying is performed until the moisture content is less than 12 wt %.

6. The Chinese medicine formula process according to any one of claims 1 to 4, characterized in that: The preparation is oral solution, tablet, pill, granule or capsule.