Tacrolimus detection kit and application thereof

By designing a tacrolimus detection kit containing magnetic bead antibodies and enzyme-labeled antigen working solution, the problems of low detection sensitivity and matrix interference in the existing technology are solved, and a more efficient and stable tacrolimus blood concentration detection is achieved, which is suitable for screening a large number of samples.

CN120761629APending Publication Date: 2025-10-10PULING BIOLOGY (NANJING) CO LTD
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Patent Information

Application Number
CN202510923847.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-04
Publication Date
2025-10-10

AI Technical Summary

Technical Problem

The existing methods for detecting tacrolimus blood concentrations have the problems of low sensitivity, poor specificity, high detection cost, complex operation and unsuitability for screening a large number of samples. In addition, the existing methods cannot effectively avoid matrix interference in blood samples, resulting in unstable test results.

Method used

A tacrolimus detection kit was designed, which contains magnetic bead antibody working solution, enzyme-labeled antigen working solution and blood sample processing solution. Detection was performed by chemiluminescence immunoassay using superparamagnetic nanomaterials and alkaline phosphatase-labeled tacrolimus antigen, combined with specific buffer and blocking solution to simplify the operation and reduce matrix interference.

Benefits of technology

The reliability and stability of tacrolimus test results are improved, the operating process is simplified, the test cost is reduced, the test time is shortened, and the reagent storage time is extended.

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Abstract

The invention discloses a tacrolimus detection kit and application thereof. The tacrolimus detection kit comprises a reagent R1, a reagent R2, a reagent R3, a standard substance, a reagent tube A, a reagent tube B, a reagent tube C and a freeze-drying bottle, wherein the reagent tube A, the reagent tube B, the reagent tube C and the freeze-drying bottle are respectively used for the reagent R1, the reagent R2, the reagent R3 and the standard substance; the reagent R1 is a magnetic bead antibody working solution; the reagent R2 is an enzyme-labeled antigen working solution; the reagent R3 is a blood sample treatment solution, which is a TBS buffer solution containing EDTA-2Na (Ethylene Diamine Tetraacetic Acid), a zinc sulfate solution, Triton X-100 and ProClin300; the standard substance is a freeze-dried whole blood standard substance containing tacrolimus. According to the tacrolimus detection kit and the application thereof, the general blood sample treating fluid is adopted, so that the reliability and the stability of a detection result of tacrolimus in a blood sample are improved.
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