Cosmetic composition containing composite mushroom extract

Abstract

The present invention provides: an emulsion-type cosmetic composition comprising an inonotus obliquus extract as a main component and a composite mushroom extract combined with an extract of at least one mushroom selected from the group consisting of an inonotus obliquus extract and Ganoderma lucidum as a liposome preparation; and a method for preparing the emulsion-type cosmetic composition. According to the present invention, the composition can be combined with a retinol compound and a combination of two hyaluronic acids of different molecular weights (mixed hyaluronic acid) and a linear oil component, such that a significant skin anti-aging or oxidation-inhibiting effect can be provided, and skin gloss can be reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to a cosmetic composition containing a complex mushroom extract. More particularly, it relates to an emulsion-type cosmetic composition and a method for preparing the same, which contains white birch mushroom extract as a main ingredient, a complex mushroom extract (mixed mushroom extract) combining extracts of at least one mushroom selected from the group consisting of white birch mushroom extract and ganoderma lucidum, etc. as a liposome formulation, a combination (mixed hyaluronic acid) of a retinoid compound and hyaluronic acid having two different molecular weights, and a straight-chain oil ingredient, can provide a significant oxidation inhibition effect, and can also reduce skin gloss. BACKGROUND

[0002] Human skin is composed of an epidermis layer, a dermis layer, and a subcutaneous tissue layer, and functions as a physical barrier to protect the body from various environmental factors, tactile sensation, temperature control, etc. At this time, the subcutaneous tissue layer is a flat layer located just below the epidermis layer and the dermis layer, and is mainly composed of adipocytes, nerves, and blood vessels.

[0003] When exposed to external stimuli, the skin is mainly protected by the sebum film, secondarily by the stratum corneum, and in severe cases by the collagen fibers of the dermis layer. The dermis layer accounts for about 85% of the thickness of the skin and is composed of collagen fibers and elastic fibers. Among them, the collagen fibers are composed of thick and thin fiber bundles of collagen, and the elastic fibers function to connect the collagen and provide elasticity to the skin and are mainly composed of elastin.

[0004] Human skin changes due to various factors such as stress, environmental pollution, and an increase in the amount of ultraviolet rays, among which the most representative changes include skin damage and functional decline due to aging, exposure to ultraviolet rays (photoaging), etc. In addition, abnormal changes in the skin, i.e., skin damage, appear in the form of dry skin, wrinkles, sagging skin, pigmentation, etc.

[0005] In order to reduce such skin damage and water loss, cosmetics containing various skin-protecting ingredients such as vitamins, flavonoids, tocopherols, and coenzyme Q10 are being actively researched, and in particular, cosmetics with low toxicity and complex functions (for example, there is a great interest in natural cosmetic ingredients having antioxidant, anti-inflammatory, anti-aging, skin moisturizing, etc. properties. In particular, it is known that natural ingredients can provide a variety of effects such as antioxidant, anti-wrinkle, whitening, and anti-itching, while minimizing side effects caused by chemicals.

[0006] In this regard, since mushrooms absorb and utilize nutrients from organic matter containing various sources of nutrients, the types of components contained in mushrooms are also diverse, and polysaccharides are the main components. In addition, mushrooms are rich in proteins, vitamins, and minerals, and have effects such as anti-inflammatory, antioxidant, and antibacterial effects. These components in mushrooms, especially polysaccharides, can act as a barrier to various harmful substances. In particular, the bioactive components contained in mushrooms not only inhibit skin aging by maintaining the inherent functions of the skin and activating skin cells, but also have excellent whitening effects by moisturizing, inhibiting melanin production, and blocking ultraviolet rays. Expanding the use of mushrooms in the existing medical and food nutrition fields and applying them to the manufacture of cosmetics using these properties are being widely researched (e.g., Korean Patent No. 681703, etc.). It is used to isolate active ingredients and introduce them into cosmetics. However, not only the degree of absorption, stability, and sustainability of each active ingredient vary depending on the layer structure of the skin, but also there are limitations in using only a single mushroom to simultaneously improve the combined effects.

[0007] In addition, in addition to improving the functionality of cosmetics, the aesthetic properties after use of cosmetics are also attracting the attention of consumers. Specifically, the skin, especially the facial skin, releases sebum components, which are undesirable from an aesthetic point of view because they produce a shine. Conventional mushroom-containing cosmetics mainly focus on functional effects, but by introducing inorganic particles, the aesthetic problems such as shine caused by the sebum components discharged from the skin can be alleviated. However, the particles introduced into the cosmetic formulation aggregate and cause problems such as the generation of irregular bumps or the clogging of skin pores on the surface of the applied cosmetic film.

[0008] As described above, techniques for applying mushroom extracts, etc. to the cosmetic field are known, but each of these natural ingredients has limitations in skin improvement effects and application. Specifically, in order to meet the increasing demand of consumers for cosmetics in recent times, a single formulation of a cosmetic that can maximize functions such as antioxidant capacity while solving existing aesthetic problems such as shine when applied to the skin is required. SUMMARY

[0009] The present inventors conducted continuous research to overcome the limitations of the above-described prior art, as a result of which an emulsion-type cosmetic composition was prepared, which prepared a mixed mushroom extract in which a white birch mushroom extract was combined with at least one mushroom extract including Ganoderma lucidum into an emulsion-type cosmetic composition containing a liposome of a retinol-based compound, hyaluronic acid containing 2 different molecular weights (mixed hyaluronic acid), and a straight-chain oil component, in which it was found that while providing functions significantly exceeding the expected level of a single mushroom extract, especially antioxidant activity, it was also possible to provide an effect that maximally reduces the shine phenomenon caused by sebum discharge after skin application.

[0010] Also, an object of the present application is to provide a cosmetic composition which not only provides improved functionality compared to the expected level of a conventional mushroom raw material extract, but also can inhibit the oil light phenomenon.

[0011] In addition, an object of the present application is to provide a preparation method for preparing the cosmetic composition.

[0012] According to an embodiment of the present application, there is provided an emulsion-type cosmetic composition, comprising: (i) 0.5 to 15 wt% of a liposome of a mixed mushroom extract, including a combination of white birch mushroom extract and ganoderma extract at a weight ratio of 1.5 to 4:1, on a powder basis; (ii) 1 to 7 wt% of a liposome of retinol, retinoids, and / or derivatives thereof; (iii) 3 to 8 wt% of a mixed hyaluronic acid of hyaluronic acid having a molecular weight (Mw) of 1000 to 2000 kDa and hyaluronic acid having a molecular weight (Mw) of 10 to 500 kDa, combined at a weight ratio of 0.5 to 3:1; (iv) 0.5 to 70 wt% of a linear oil represented by the following Formula 1; and (v) a balance of water:

[0013] [Formula 1]

[0014] (CH3)3Si-O-[Si(CH3)2-O] n -Si(CH3)3

[0015] In the above formula, n is 0 to 4.

[0016] According to an exemplary embodiment, the mixed mushroom extract further includes shiitake mushroom extract, in which case the white birch mushroom extract: shiitake mushroom extract can be at a weight ratio of 8 to 15:1, on a powder basis.

[0017] According to an exemplary embodiment, the linear oil can have a kinematic viscosity of 0.3 to 10 cSt (25°C).

[0018] According to another embodiment of the present application, there is provided a preparation method of an emulsion-type cosmetic composition, characterized by comprising: a) a step of mixing white birch mushroom extract and ganoderma extract to prepare a liposome of a mixed mushroom extract; b) a step of separately preparing a liposome of retinol, retinoids, and / or derivatives thereof; and c) a step of mixing the liposome of the mixed mushroom extract, the liposome of retinol, retinoids, and / or derivatives thereof, and mixing a mixed hyaluronic acid, a linear oil, and water under the condition of applying a shearing force, the composition comprising:

[0019] (i) 0.5 to 15% by weight of liposomes of a mixed mushroom extract, including a combination of white birch mushroom extract and ganoderma extract at a weight ratio of 1.5 to 4:1, based on the powder; (ii) 1 to 5% by weight of liposomes of retinol, retinoids, and / or derivatives thereof; (iii) 3 to 8% by weight of a mixed hyaluronic acid combining hyaluronic acid having a molecular weight (Mw) of 1000 to 2000 kDa and hyaluronic acid having a molecular weight (Mw) of 10 to 500 kDa at a weight ratio of 0.5 to 3:1; (iv) 0.5 to 70% by weight of a linear oil represented by the following Formula 1; and (v) the balance water:

[0020] [Formula 1]

[0021] (CH3)3Si-O-[Si(CH3)2-O] n -Si(CH3)3

[0022] In the above formula, n is 0 to 4.

[0023] The extraction solvent used to provide the white birch mushroom extract and the ganoderma extract in step a) can be at least one selected from the group consisting of water, methanol, ethanol, isopropanol, butanol, acetone, hexane, diethyl ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butanediol, and propylene glycol.

[0024] According to an exemplary embodiment, the extraction to obtain the white birch mushroom extract in step a) can include a step of using 2 to 20 times the weight of water as an extraction solvent by weight, extracting the white birch mushroom powder at least once for 0.5 to 50 hours at 10 to 80°C to obtain an extract.

[0025] According to an exemplary embodiment, the extraction to obtain the white birch mushroom extract in step a) can include a step of using 2 to 20 times the weight of water as an extraction solvent by weight, extracting the white birch mushroom powder at least once for 0.5 to 50 hours at 10 to 80°C to obtain an extract.

[0026] According to an exemplary embodiment, the extraction to obtain the white birch mushroom extract in step a) can include a step of using 2 to 20 times the weight of water as an extraction solvent by weight, extracting the white birch mushroom powder at least once for 0.5 to 50 hours at 10 to 80°C to obtain an extract.

[0027] According to an exemplary embodiment, the alcohol can be at least one selected from the group consisting of methanol, ethanol, isopropanol, n-butanol, and 1,3-butanediol.

[0028] According to an exemplary embodiment, the white birch mushroom powder is obtained by pulverizing white birch mushroom and baking at 100 to 200℃ for 3 to 10 minutes before extraction.

[0029] The emulsion-type cosmetic composition according to the present application not only has excellent functionality such as an antioxidant effect, but also can minimize the gloss and the like caused by the oil component discharged from the skin after being applied to the skin, and has advantages in functionality and aesthetics. DETAILED DESCRIPTION

[0030] The present application can be implemented by the following description. The following description is to be understood as describing the preferred embodiments of the present application, but the present application is not necessarily limited thereto.

[0031] "Mushroom" belongs to Basidiomycetes, a higher fungus, and is biologically positioned as a fungus. Mushroom spores germinate to form mycelium, which grows into a fruit body in the shape of a mushroom, and the fruit body repeatedly forms the growth process of spores.

[0032] "Emulsion" can be understood as a concept including oil-in-water and water-in-oil.

[0033] "Liposome" can refer to a bilayer phospholipid, which is generally amphiphilic, forming a spherical vesicle, in which case a bilayer phospholipid can be formed when the lipid is introduced into an aqueous solution. The liposome can contain active ingredients (lipophilic and hydrophilic active ingredients) in the internal space of the vesicle.

[0034] "Extract" can be understood to include not only an extract obtained by directly using an extraction solvent, but also a purified extract obtained by performing additional separation and purification processes (e.g., fractionation).

[0035] "Phospholipid" can refer to a hydrophobic molecule containing at least one phosphorus (P) group, for example, can include a phosphorus-containing group substituted with OH, -COOH, oxo, an amine group, or a substituted or unsubstituted aryl group, containing a saturated or unsaturated alkyl group.

[0036] "Phosphatidylcholine" can refer to phosphatidylcholine and derivatives thereof.

[0037] In the present specification, when a numerical range is designated as a lower limit and / or an upper limit, it can be understood that any sub-combination within the numerical range is also disclosed. For example, when written as "1 to 5", it can include 1, 2, 3, 4, and 5, and any sub-combination therebetween.

[0038] In the present invention, when any component or member is "connected" to other components or members, unless otherwise specified, it can be understood to include the case of being connected through other components or members, in addition to being directly connected to the other components or members.

[0039] Similarly, the term "contact" can also be understood to include not only direct contact, but also the case of contact through other components or members.

[0040] When referring to "including" a component, unless otherwise specified, it means that other components can also be included.

[0041] As described above, the present invention is not simply to use one type of mushroom extract alone, but to capture a mixed mushroom extract, in which a white birch mushroom extract is mixed with a ganoderma extract in a predetermined ratio, in a liposome form, while combining a liposome of a vitamin A component (particularly, retinol, retinoids, and / or derivatives thereof) with two types of molecular weight of hyaluronic acid, to maximize the function of a complex cosmetic based on a mushroom extract. In addition, the emulsion-type cosmetic of the present invention, by further containing an oil component having a specific chemical structure, not only provides the aforementioned function improvement effect, but also provides a cosmetic effect, and when applied to the skin, particularly, facial skin, can inhibit the shine caused by the oil component discharged from the skin.

[0042] Liposome preparation of mushroom extract

[0043] According to an embodiment of the present invention, the cosmetic composition contains a mixed mushroom extract, in which a white birch mushroom extract is mixed with a ganoderma extract, in a form of being encapsulated in a liposome.

[0044] In this regard, the white chanterelle mushroom is a perennial basidiomycete mushroom that grows on the stems or large branches of living birch trees (among which Betula pendula and Betula pubescens are mainly inhabited), using the nutrients of the birch trees to grow. Specifically, the white chanterelle fungus decomposes the fibrous tissue and woody tissue of the birch tree, forms roots inside the birch tree, uses the wood / sap / flavonoids of the birch tree as nutrients to grow inside the birch tree, and then breaks through the bark of the birch tree and further grows. The main physiologically active ingredient of the white chanterelle is β-glucan, which is composed of stem branches (1-3) and (1-6) bonds, and has effects such as enhancing immunity. In addition, the polyphenol component of the flavonoid series has an antioxidant effect. In addition, the active ingredients of the white chanterelle contain inositol and various minerals such as manganese and zinc, etc.

[0045] In addition, Ganoderma lucidum is a mushroom belonging to the family of Basidiomycetes, and its fruiting body has been widely used as a medicinal herb in the East due to its efficacy. In particular, it is reported that the Ganoderma lucidum extract contains ingredients that regulate immune function, have an anticancer effect, and stimulate lymphocyte production. Among the components of Ganoderma lucidum, triterpenes and polysaccharides are known to be mainly important ingredients having pharmacological effects and substances having physiological activity useful for immune function.

[0046] According to an embodiment, in order to obtain a mixed mushroom extract containing white chanterelle extract and Ganoderma lucidum extract, mushroom extracts are first prepared from white chanterelle powder and Ganoderma lucidum powder, respectively. At this time, both the white chanterelle extract and the Ganoderma lucidum extract can be used as commercially available products. In alternative embodiments, white chanterelle extract and Ganoderma lucidum extract are obtained by performing extraction treatment on white chanterelle powder (pulverized material) and Ganoderma lucidum powder (pulverized material), respectively, and can be used to prepare a liposome of a mixed mushroom extract.

[0047] According to an exemplary embodiment, a mixed mushroom extract containing shiitake extract can be provided in addition to the white chanterelle extract and the Ganoderma lucidum extract. In this case, a commercially available shiitake extract can be used, or a shiitake extract can be prepared by other extraction treatment, and a mixed mushroom extract can be prepared by additionally mixing it with the aforementioned white chanterelle extract and Ganoderma lucidum extract. In this regard, shiitake is a mushroom belonging to the genus of Pleurotus and Tricholoma of the order of Basidiomycetes, and is a mushroom that naturally grows on dry trees such as chestnut trees, oak trees, or oak trees. In particular, it can effectively prevent hypertension and arteriosclerosis because it can lower cholesterol levels and lower blood pressure. The aroma component of shiitake appears due to the decomposition of oxynitric acid. Shiitake is rich in vitamin D, and has good antioxidant and anti-aging activity.

[0048] According to exemplary embodiments, in the case of white birch mushroom extract, in order to improve the extraction efficiency of β-glucan in white birch mushroom during the extraction process and increase the content of β-glucan in the extract, the white birch mushroom can be optionally ground into powder form before roasting. As an example, the roasting temperature can be adjusted in the range of, for example, about 100°C to 200°C, specifically about 120°C to 180°C, and more specifically about 150°C to 170°C. In addition, the roasting time can be, for example, about 3 to 10 minutes, specifically about 4 to 9 minutes, and more specifically about 5 to 8 minutes.

[0049] As an example, extraction can be applied, for example, cold soak extraction, ultrasonic extraction, reflux extraction, hot water extraction, etc. Specifically, solvent extraction using an extraction solvent can be applied, and specifically, extraction can be performed using water, an organic solvent, or a mixed solvent thereof. As an example, the extraction solvent can be, for example, at least one selected from the group consisting of water, methanol, ethanol, isopropanol, butanol, acetone, hexane, diethyl ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butanediol, and propylene glycol. Depending on the extraction solvent, the extraction efficiency of the active ingredients in the mushroom mixture can be affected, and thus it is recommended to select a suitable solvent.

[0050] According to exemplary embodiments, white birch mushroom extract can be obtained by the methods exemplified below.

[0051] First, water can be used as an extraction solvent to extract white birch mushroom powder under relatively low temperature conditions. This is in consideration of the fact that the active ingredients in white birch mushroom are heat sensitive and can be destroyed. According to one exemplary embodiment, the extraction temperature can be in the range of, for example, about 10 to 80°C, specifically about 15 to 60°C, more specifically 20 to 40°C, and in some cases, can be room temperature. Temperature conditions. At this time, the amount of extraction solvent is adjusted to, for example, about 2 to 20 times, specifically about 5 to 15 times, more specifically about 7 to 10 times, the weight of the white birch mushroom powder. This is the extraction target. In addition, extraction can be performed at least once, and the total extraction time can be adjusted in the range of about 0.5 to 50 hours, specifically about 5 to 40 hours, and more specifically about 10 to 30 hours.

[0052] As described above, the extract obtained by water extraction is in a liquid form obtained by removing impurities by filtration (e.g., ultrafiltration, high-performance liquid chromatography, etc.) according to a conventional method, or the extract in a liquid form obtained. The extract in powder form can be obtained by concentration (e.g., concentration under reduced pressure) (i.e., in the form of a concentrated extract) or additional drying (specifically, freeze-drying, spray-drying, etc.). If necessary, these extracts can be diluted to facilitate handling or processing.

[0053] According to an alternative embodiment, the white birch mushroom extract can be obtained by using organic solvent extraction, and in this case, the isolation and recovery of active ingredients such as β-glucan can be maximized.

[0054] As an example, extraction of white birch mushroom powder is first performed using alcohol as an extraction solvent (primary extraction). At this time, the alcohol can be at least one selected from the group consisting of methanol, ethanol, isopropanol, n-butanol, and 1,3-butanediol. In certain embodiments, the alcohol can be ethanol, isopropanol, and / or n-butanol, and more specifically, ethanol can be used. In the case of such alcohol extraction, the amount of the extraction solvent used can be adjusted in the range of about 5 to 10 times, specifically about 6 to 9 times, and more specifically about 7 to 8 times, based on the weight of the mushroom powder. In addition, at least one extraction can be performed to obtain a crude extract. Next, a first concentrate can be obtained by concentrating (e.g., concentration under reduced pressure) the crude extract obtained by one extraction.

[0055] Next, the first concentrate is suspended or dispersed in water (specifically, distilled water), and then extracted with an organic solvent (secondary extraction). At this time, the amount of water used can be adjusted in the range of, for example, about 20 to 70 times, specifically about 30 to 60 times, more specifically about 35 to 55 times, based on the volume, compared to the first time. Concentration. In addition, the organic solvent as the extraction solvent can be, for example, chloroform and / or ethyl acetate. In this case, the amount of the organic solvent used is, for example, about 30 to 100 times, specifically about 50 to 90 times, more specifically about 60 to 80 times, by volume compared to the first concentrate. The extract obtained by the above-mentioned secondary extraction can be concentrated to obtain a second concentrate.

[0056] The second concentrate is dissolved in ethyl acetate, and then passed through a silica gel bead to adsorb and remove non-active fractions, thereby eluting only active fractions. At this time, the particle size of the silica gel bead is not particularly limited, but can be set in the range of, for example, about 100 μm to 5000 μm, specifically about 200 μm to 3000 μm, more specifically about 500 μm to 1500 μm. Subsequently, the active fraction is concentrated (e.g., concentration under reduced pressure) to obtain a third concentrate. In this way, the obtained third concentrate can be suspended or dispersed by adding water. In this case, the amount of water added is about 2 to 5 times, specifically about 2.5 to 4.5 times, of the volume of the third concentrate. It can be adjusted in the range of about 3 to 4 times. Next, the suspension can be concentrated to obtain a white birch mushroom extract, and if necessary, it can be prepared in powder form by the above-mentioned drying (specifically, freeze-drying, spray-drying, etc.) process.

[0057] In the present embodiment, the weight ratio of the Chaga mushroom extract to the Ganoderma extract (powder or solid base) in the mixed mushroom extract can be, for example, about 1.5 to 4: 1, specifically about 2 to 3.5: 1, and more specifically about 2.5 to 3: 1. In this way, by controlling the ratio of the Chaga mushroom extract and the Ganoderma extract, the Chaga mushroom's unique high activity can provide a balanced function of antioxidant and antibacterial (or antiviral) effects, and the Ganoderma's high immunity-enhancing effect, and in particular, the synergistic effect with the Ganoderma extract can significantly improve the antioxidant effect. If the combination ratio is outside the above range, the compatibility between the extracts decreases, making it difficult to achieve a synergistic effect by the combination.

[0058] In addition, if more Shiitake extract is contained, the weight ratio of the Chaga mushroom extract to the Shiitake extract (powder or solid base) in the mixed mushroom extract can be adjusted in the range of, for example, about 8 to 15: 1, specifically about 9 to 13: 1, more specifically about 10 to 12: 1. In this way, by further including the Shiitake extract, the mixed mushroom extract can not only provide an enhanced antioxidant effect, but also provide an additional vitamin D-related effect and an anti-aging effect.

[0059] According to an embodiment, the mixed mushroom extract is used in the form of a liposome. The liposome is a closed vesicle made of a phospholipid bilayer membrane, and in the present embodiment, the mixed mushroom extract is captured in the inner space of the bilayer membrane. According to the present embodiment, a phospholipid is used to form the cell layer of the liposome, and the mixed mushroom extract is loaded inside thereof. In this regard, the liposome can be prepared using the methods known in the art, such as the film hydration method, the ethanol injection method, the ether injection method, the freeze-thaw method, etc., alone or in combination. As one example, it can be prepared according to the method shown below.

[0060] According to the exemplary embodiment, the phospholipid is first added to a solvent and a suspension (or dispersion) containing the above-described mixed mushroom extract is prepared. At this time, the phospholipid has good stability and storage characteristics, does not cause an off-flavor (i.e., maintains the effect of the active ingredient even under changes in pH and / or temperature), and in particular, is capable of effectively delivering the active ingredient into the body. It can be selected from the types having characteristics, for example, can be at least one selected from the group consisting of egg yolk lecithin, soybean lecithin, lyso-lecithin, phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidic acid, etc. Specifically, it can be a compound based on phosphatidylcholine, more specifically phosphatidylcholine, lyso-phosphatidylcholine, and / or hydrogenated phosphatidylcholine.

[0061] According to an exemplary embodiment, the solvent can be at least one organic solvent selected from the group consisting of an alcohol (specifically, an alcohol having 1 to 4 carbon atoms, more specifically, ethanol), chloroform, tetrahydrofuran, and the like. In particular, chloroform and / or tetrahydrofuran can be used. At this time, the concentration of the phospholipid in the suspension can be adjusted, for example, in the range of about 0.5 to 6% (w / v), specifically about 1 to 5% (w / v), more specifically about 2 to 4% (w / v). If the phospholipid concentration is too large or too small, the phospholipid film can not be hydrated, or the formation of liposomes with uneven particle size can be increased, and thus it can be advantageous to adjust it appropriately in the above range.

[0062] Then, the solvent in the mixture can be removed to form a dried lipid film (or membrane), and for this, an evaporation device such as a rotary evaporator can be applied. In addition, the temperature during evaporation can be adjusted in the range of, for example, about 45°C to 70°C, specifically about 50°C to 55°C.

[0063] Thereafter, the formed film can be hydrated by adding an aqueous medium such as water, particularly distilled water, and uniformly dispersed using a dispersion device known in the art, such as a sonicator, a homogenizer, or a microfludizer, etc., to form a suspension (or dispersion) of liposomes.

[0064] According to an exemplary embodiment, the production of liposomes can involve sonication, and at this time, the frequency of the irradiated ultrasound is, for example, about 10 to 100 kHz, specifically about 20 to 50 kHz, more specifically about 25 to 40 kHz. In addition, the sonication time can be adjusted in, for example, about 1 hour, specifically about 0.3 to 0.9 hours, more specifically about 0.6 to 0.8 hours. Next, the suspension of liposomes can be obtained by typical post-treatment such as centrifugal separation, etc., and, in some cases, in order to be used for subsequent use, the dispersion medium can be removed and stored after drying (e.g., reduced pressure drying, freeze drying, etc.).

[0065] The liposomes prepared by this method can have a nanoscale, and as an example, the average size (diameter) of the liposomes can be in the range of, for example, about 10 to 2000 nm, specifically about 50 to 1000 nm, more specifically about 100 to 500 nm, which can be adjusted considering the storage capacity, skin delivery ability, etc. At this time, the size of the liposomes can be calculated by measuring the size of individual particles using a transmission electron microscope and then averaging them.

[0066] According to an exemplary embodiment, the weight ratio of the mixed mushroom extract in the liposome: the cell layer (containing phospholipids) can be adjusted considering molecular weight, viscosity, etc., for example, within the range of 1: about 7 to 20, specifically 1: about 10 to 18, and more specifically 1: about 12 to 15.

[0067] In addition, the absolute value of the zeta potential of the liposomes capturing the mixed mushroom extract may be at least about 30 mV, specifically at least about 40 mV, and more specifically within the range of about 45 to 60 mV, and thus, aggregation between liposomes may be effectively suppressed.

[0068] In this way, when the mixed mushroom extract is captured and used in the form of liposomes, the mixed mushroom extract is kept in a dispersed state as much as possible (i.e., by inhibiting aggregation), thereby improving its antioxidant and anti-inflammatory effects. At the same time, the mixed mushroom extract can be contained in water, which can effectively increase the solubility and improve the storage and stability of cosmetics in the form of emulsions, especially oil-in-water emulsions.

[0069] According to one embodiment, the liposome content of the mixed mushroom extract in the cosmetic composition may be set within a range of about 0.5 to 15 wt%, specifically about 2 to 12 wt%, and more specifically about 5 to 10 wt%. If the liposome content of the mixed mushroom extract is below a certain level, it may be difficult to ensure the desired function.

[0070] Liposomes of retinol, retinoids and / or derivatives thereof

[0071] According to the present invention, vitamin A ingredients with anti-aging and wrinkle-improving properties, such as retinol-based ingredients, particularly retinol, retinoids, and / or their derivatives, are incorporated into cosmetic compositions. As described above, these ingredients are encapsulated in liposomes to maximize skin delivery.

[0072] Retinoids, particularly retinol, are essential nutrients composed of a cyclic head group, a polyene chain, and an alcohol end group. However, while retinoids are soluble in organic solvents (such as ethanol), fats, and oils, their solubility in water is low. Consequently, when used in cosmetics containing water, particularly skincare toners with high water content, these compounds can suffer from reduced functionality and storage stability due to low stability.

[0073] In consideration of this, in this specific example, not only the mushroom extract is mixed but also the retinol compound is captured in the form of liposomes and included in the cosmetic composition.

[0074] According to an exemplary embodiment, in order to prepare the liposome, a phospholipid can be used as described above, and a sterol can be optionally combined therewith. At this time, the sterol can be, for example, at least one selected from the group consisting of cholesterol, β-sitosterol, stigmasterol, lanosterol, brassicasterol, campesterol, ergosterol, chalinosterol, sitosterol, avenasterol, sarinosterol, fucosterol, and the like, and specifically can be cholesterol. As such, when the phospholipid and the sterol are used in combination, the sterol component forms a hydrogen bond with the phospholipid, reducing the fluidity of the phospholipid membrane, thereby increasing the stability, and also can inhibit oxidation and elution of the active ingredient due to heating during the manufacturing process at the cosmetic counter.

[0075] In an exemplary embodiment, the weight ratio of the phospholipid and the sterol can be in the range of, for example, about 4 to 12: 1, specifically about 5 to 10: 1, and more specifically about 6 to 9: 1, and, preferably, can be appropriately adjusted within the above range in consideration of the capture efficiency, stability, and the like.

[0076] According to an exemplary embodiment, the liposome of the retinol compound can also have a nanoscale, and as an example, the average size (diameter) of the liposome is, for example, about 30 to 1000 μm, specifically about 70 to 800 μm. More specifically, about 100 to 400 nm. In addition, the absolute value of the ZETA potential of the liposome of the retinol compound can be at least about 30 mV, specifically at least about 40 mV, and more specifically in the range of about 45 to 60 mV.

[0077] According to an embodiment, the content of the liposome of the retinol compound in the cosmetic composition can be set in the range of about 1 to 7% by weight, specifically about 2 to 5% by weight, and more specifically about 2.5 to 4% by weight. If the content of the liposome of the retinol compound is lower than a certain level, it is difficult to sufficiently provide the functions required to be achieved by retinol, etc., and if the content is too high, the degree of improvement of the additional functions is small, and thus is not desirable in terms of economic efficiency.

[0078] Mixed hyaluronic acid

[0079] According to an embodiment, the emulsion system cosmetic composition further contains a combination of two hyaluronic acids according to the molecular weight, i.e., a mixed hyaluronic acid.

[0080] Hyaluronic acid is a natural linear polysaccharide, and is one of glycosaminoglycans, and is composed of repeating disaccharide units of N-acetylglucosamine and glucuronic acid. At this time, the hyaluronic acid can be obtained, for example, by culturing lactic acid bacteria. There are polar and non-polar fragments within the hyaluronic acid, providing a characteristic of chemically interacting with various compounds.

[0081] According to the present embodiment, mixed hyaluronic acid is used, which combines high molecular weight hyaluronic acid and low molecular weight hyaluronic acid in a predetermined ratio. The molecular weight of hyaluronic acid is the molecular weight of the liquid medium or dispersion containing it. It not only affects rheological properties, but also affects function (such as anti-inflammatory effect). In this regard, high molecular weight hyaluronic acid is a higher antioxidant and anti-inflammatory effect, but as the polymer network becomes denser, viscosity increases, and fluidity decreases, which can reduce miscibility with other ingredients, stability and applicability. Hyaluronic acid can reduce viscosity and increase fluidity, but will increase oxidation and inflammation instead.

[0082] Taking this into account, in this embodiment, high molecular weight hyaluronic acid and low molecular weight hyaluronic acid are combined in a predetermined ratio to maximize the effect of hyaluronic acid itself while maintaining the rheological properties at an appropriate level. To this end, the molecular weight (Mw) of the high molecular weight hyaluronic acid can be adjusted within the range of about 1000 to 2000 kDa, specifically about 1200 to 1800 kDa, more specifically about 1500 to 1750 kDa, and the molecular weight (Mw) of the low molecular weight hyaluronic acid can be adjusted within the range of about 100 to 500 kDa, specifically about 150 to 450 kDa, more specifically about 200 to 400 kDa.

[0083] According to one embodiment, the combined ratio (by weight) of high molecular weight hyaluronic acid to low molecular weight hyaluronic acid is adjusted to, for example, a range of approximately 0.5 to 3:1, specifically approximately 1 to 2.5:1, and more specifically approximately 1.5 to 2:1. If the ratio of high molecular weight hyaluronic acid to low molecular weight hyaluronic acid is too high, problems such as reduced compatibility with other ingredients in the formulation may arise, while if the ratio is too low, functionality may be reduced. Therefore, it is preferably adjusted within the above range.

[0084] According to one embodiment, the content of the mixed hyaluronic acid in the cosmetic composition can be set within a range of about 3% to 8% by weight, specifically about 3.5% to 7% by weight, and more specifically about 4% to 6% by weight. Since the content of the mixed hyaluronic acid in the cosmetic formulation affects flow properties, miscibility, etc., it is desirable to appropriately adjust it within the above range.

[0085] Straight chain oils

[0086] According to one embodiment of the present invention, a predetermined oil is included in a cosmetic composition containing water in order to suppress shine caused by oil discharged from the skin and a film-like cosmetic formed on the skin surface after application.

[0087] In this regard, the oil component added to the cosmetic composition is a straight-chain oil, which is represented by the following formula 1:

[0088] [Formula 1]

[0089] (CH3)3Si-O-[Si(CH3)2-O] n -Si(CH3)3

[0090] In the above formula, n is 0 to 4 (specifically, 1 to 3).

[0091] The above linear oil refers to an oil having silicon in the chemical structure and at least two siloxanes in the molecular structure. In addition, the kinematic viscosity (25°C) of the linear oil can be in the range of, for example, about 0.3 to 10 cSt, specifically about 0.5 to 5 cSt, more specifically about 0.6 to 1 cSt. A representative example of such an oil can be hexamethyldisiloxane.

[0092] Preparation of cosmetic compositions

[0093] According to other embodiments, the above liposome of mixed mushroom extract, liposome of retinol compound, mixed hyaluronic acid, linear oil, etc. can be combined with water to prepare an emulsion-type cosmetic.

[0094] According to exemplary embodiments, the previously prepared liposome of mixed mushroom extract and liposome of retinol compound are first mixed, and then the mixed hyaluronic acid, linear oil, and water are mixed in a predetermined ratio and mixed with the application of shear force to prepare an emulsion preparation.

[0095] If the content of the liposome of mixed mushroom extract, liposome of retinol compound, mixed hyaluronic acid, oil component, etc. is determined in such a way as to have the required composition range, water can be used as the remaining amount. At this time, purified water, deionized water, distilled water, etc. can be used as water, but there is no particular limitation.

[0096] Devices that apply shear force when mixing the above various components are known in the art, and a homogenizer, a mixer (homogenizer), a high-speed mixer, etc. can be used.

[0097] In this regard, in order to suppress the deterioration of the cosmetic components during the mixing process, it is preferable not to set a temperature that is too high, for example, in the range of about 15 to 40°C, specifically about 18 to 35°C, more specifically about 20 to 30°C. In certain embodiments, it can be set to room temperature.

[0098] These cosmetic compositions can be prepared in the form of any preparation known in the art, such as a suspension, an emulsion, a paste, a gel, a cream, a lotion, a cleanser, or a spray. Specifically, examples include an emulsion, a cream, an essence, a cleansing foam, a makeup remover, a moisturizer, a moisturizing oil, a shower gel, a shampoo, a hair conditioner, a hair conditioner, etc. In addition, known excipients or additives, typically stabilizers, solubilizers, vitamins, pigments, fragrances, etc. can also be included.

[0099] For example, in the case of a solution or emulsion, a solvent, solubilizer or emulsifier can be used as a carrier component, specifically water, ethanol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol fatty esters, polyethylene glycol and / or fatty acid esters of sorbitan.

[0100] In addition, it may include a binder containing starch, tragacanth gum, gelatin, molasses, polyvinyl alcohol, polyvinyl ether, polyvinyl pyrrolidone, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose and carboxymethyl cellulose; an abrasive containing agar, starch, gelatin powder, sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, crystalline cellulose, calcium carbonate, sodium bicarbonate, sodium alginate; a lubricant containing magnesium stearate, talc and hydrogenated vegetable oil; and a coloring agent, etc.

[0101] As the carrier, lactose, glucose, sucrose, mannitol, potato starch, corn starch, calcium carbonate, calcium phosphate, cellulose, etc. can be used.

[0102] Furthermore, additives such as stabilizers, solubilizers, transdermal absorption enhancers, and the like, as well as fragrances, preservatives, and the like may be contained.

[0103] According to an exemplary embodiment, the aforementioned additional ingredients, for example, may comprise up to about 20 wt %, specifically up to about 15 wt %, based on the cosmetic composition, but this is understood as an example.

[0104] The present invention can be more clearly understood through the following examples, which are only used for illustrative purposes to illustrate the present invention and are not intended to limit the scope of the present invention.

[0105] Example

[0106] Cosmetic compositions

[0107] 1. Liposomes of Mushroom Extract

[0108] Ganoderma lucidum extract and Lentinus edodes extract were obtained as commercially available products and used, while Chaga extract was prepared according to the following procedure.

[0109] First, commercially available Chaga mushrooms were ground into a powder using a grinding device and then baked at 130°C for 5 minutes. 500g of the baked Chaga mushroom powder was added to 3.5L of ethanol (70% ethanol in water) and refluxed for 5 hours. The extract from the ethanol solvent was then concentrated under reduced pressure to produce a primary concentrate. Thereafter, approximately 50 volumes of distilled water were added and dispersed into the primary concentrate, and the primary concentrate was then extracted a total of three times with approximately 70 volumes of chloroform. The organic layer was then concentrated under reduced pressure to produce a secondary concentrate.

[0110] The second concentrate was dissolved in ethyl acetate and then passed through silica gel resin beads to elute only the active fraction with ethyl acetate, followed by concentration under reduced pressure to obtain a third concentrate.

[0111] Next, about 3 times the volume of distilled water was added to the third concentrate to disperse it uniformly, and then concentrated under reduced pressure and freeze-dried to obtain a powdered Chaga extract.

[0112] -Liposomes mixed with mushroom extracts (Mushroom Extract Liposome 1)

[0113] The obtained powdered Chaga extract was mixed with Ganoderma lucidum extract in a weight ratio of 3:1 to prepare a mixed mushroom extract, and liposomes were prepared using a thin film hydration method. Specifically, phosphatidylcholine was dissolved in chloroform to 2% (w / v), and then the mixed mushroom extract was added and dissolved. At this time, an ultrasonic treatment device was used to treat the mixture with ultrasound at 30 kHz and 40% amplitude for 40 minutes. Then, a rotary evaporator was used to evaporate the chloroform to form a lipid mixed film, distilled water was added, and then ultrasound treatment was performed for 30 minutes to collect the mixed mushroom extract. Liposomes (mushroom liposome 1) were prepared. The particle size of the liposomes was measured using a particle size analyzer, and the particle size of the liposomes containing the mixed mushroom extract was found to be approximately 320 nm. In addition, the liposome capture efficiency was approximately 91.2%.

[0114] -Liposomes mixed with mushroom extracts (Mushroom Extract Liposome 2)

[0115] In addition to preparing a liposome formulation containing shiitake mushroom extract in addition to Chaga and Ganoderma lucidum extracts, liposomes containing mixed mushroom extracts were prepared using the same procedure as above. In this case, the ratio of Chaga to Shiitake extract was 12:1, resulting in a liposome size of approximately 350 nm and a liposome capture efficiency of approximately 89.7%.

[0116] - Liposomes of Chaga extract (Liposomes of Mushroom Extract 3)

[0117] Liposomes containing Chaga extract were prepared using the same procedure as above, except that the liposome preparation was prepared using Chaga extract alone. At this time, the liposome size was approximately 300 nm, and the liposome capture efficiency was approximately 93.1%.

[0118] - Liposomes of Ganoderma lucidum extract (Liposomes of Mushroom Extract 4)

[0119] The same procedure was used to prepare liposomes containing Ganoderma lucidum extract, except that Ganoderma lucidum extract alone was used to prepare the liposome preparation. At this time, the size of the liposomes was about 420 nm, and the liposome capture efficiency was about 85.2%.

[0120] 2. Retinol Liposomes

[0121] Phosphatidylcholine, cholesterol, and retinol were added to a glass flask and dissolved in 99.9% ethanol as an organic solvent to prepare a mixed solution. The weight ratio of phosphatidylcholine to cholesterol was set at 7:1. The organic solvent in the mixture was evaporated to form a film, which was then hydrated by adding an equal volume of distilled water. The film was then sonicated at 30 kHz and 40% amplitude using a sonicator to prepare retinol liposomes.

[0122] 3. Hyaluronic acid

[0123] -Hyaluronic acid 1

[0124] Hyaluronic acid was used in which a high molecular weight hyaluronic acid having a molecular weight (Mw) of 1550 kDa and a low molecular weight hyaluronic acid having a molecular weight (Mw) of 240 kDa were combined at a weight ratio of 1.7:1.

[0125] -Hyaluronic acid 2

[0126] High molecular weight (Mw) hyaluronic acid with a molecular weight (Mw) of 1550 kDa was used.

[0127] -Hyaluronic acid 3

[0128] Low molecular weight hyaluronic acid with a molecular weight (Mw) of 240 kDa was used.

[0129] 4. Straight chain oil

[0130] Hexamethyldisiloxane was used as the oil component.

[0131] Preparation of cosmetic compositions

[0132] Mixed mushroom extract, liposomes of retinol, mixed hyaluronic acid, and linear oil were adjusted in various ratios and mixed with water as shown in Table 1 below.

[0133]

Table 1

[0134]

[0135] Evaluation

[0136] 1. Evaluation of antioxidant activity

[0137] -Free radical scavenging activity using the DPPH method

[0138] 250 μL of the solution was added to a concentration of 5 × 10 -4A 10 μL DPPH (2,2-diphenyl-1-trinitrophenylhydrazine) solution (dissolved in 99% ethanol) was added to a 50 μL sample of the cosmetic composition, mixed, and then maintained at room temperature for 1 hour. Next, the electron donation capacity of the sample-added solution was measured at 517 nm absorbance. The free radical scavenging ability was evaluated based on the reduced absorbance compared to the positive control (ascorbic acid). The results are shown in Table 2.

[0139] -ABTS free radical scavenging activity

[0140] Before using the blue / green radical cation (ABTS+) of ABTS produced by reacting 7 mM ABTS with 2.5 mM potassium persulfate, the sample was left in the dark at room temperature for 12 hours. A 10 μL sample was added to the previously prepared radical cation (ABTS+) solution, allowed to react for 30 minutes, and then the absorbance was measured at 750 nm. The decrease in absorbance corresponding to the degree of free radical elimination by the sample was measured, and ascorbic acid was used as a positive control for activity comparison. The results are shown in Table 2 below.

[0141] -Superoxide radical scavenging activity

[0142] A reaction mixture containing 20 μl of 15 mM Na₂EDTA, 50 μl of 0.6 mM NBT, 30 μl of 3 mM hypoxanthine, 50 μl of xanthine oxidase (1 unit in 10 mL of buffer), 5 μl of sample, and 145 μl of buffer (50 mM KH₂PO₄ / KOH) was used. The reaction was initiated by adding xanthine oxidase at 25°C, and the absorbance was measured at 570 nm. As a positive control for comparing activity, ascorbic acid was used. The results are shown in Table 2.

[0143] 2. Miscibility and stability testing

[0144] To evaluate the compatibility and stability of the ingredients in cosmetic composition samples, discoloration and layer separation were observed over a period of time under specific conditions. In this experiment, the cosmetic composition samples were placed in a constant-temperature chamber at 45°C and visually observed for 30 days to detect any cloudiness or precipitation. The results are shown in Table 2.

[0145]

Table 2

[0146]

[0147]

[0148] Referring to the above table, as a result of the evaluation of the 3 radical scavenging activities, when the liposome of the mixed mushroom extract in which the white birch mushroom extract and the ganoderma extract were combined at a predetermined ratio according to the present application was used (Example 1), and when the liposome of the mixed mushroom extract further containing the shiitake extract was used (Example 2), good scavenging activities were shown. In particular, when the additional shiitake extract was contained, relatively higher scavenging activities were shown, which is considered to be because a balanced function was provided between the active ingredients in the three mushroom extracts.

[0149] On the other hand, when the liposome containing only the white birch mushroom extract was contained (Comparative Example 1), the scavenging activity was relatively low compared to the examples. In addition, when the liposome containing only the ganoderma extract was contained (Comparative Example 2), the radical scavenging activity was significantly lower than the examples and Comparative Example 1, which is considered to be derived from the lack of the antioxidant effect of the white birch mushroom and the decrease in effectiveness due to the use of only the low molecular weight hyaluronic acid.

[0150] In addition, when only the high molecular weight hyaluronic acid (hyaluronic acid 2) was used (Comparative Example 1), precipitation occurred, which is considered to be due to the relatively low miscibility with other ingredients, resulting in a decrease in stability.

[0151] Evaluation of gloss

[0152] Twenty 20- to 40-year-old women (10 with oily skin, 10 with mixed skin) and twenty 20- to 40-year-old men (10 with oily skin, 10 with mixed skin) were selected as subjects, and the cosmetic compositions according to Examples 1 and 2 and Comparative Examples 1 to 3 were applied to both sides of the face, the time at which gloss was evaluated to be shown was measured, and the average was calculated. The results thereof are shown in Table 3.

[0153]

Table 3

[0154]

[0155]

[0156] Referring to the above table, in the case of the cosmetic composition according to the examples, the gloss phenomenon felt by the testers could be inhibited until a relatively long time elapsed after being applied to the skin. In particular, it can be seen that although 50% of the testers had oily skin and would expel the oil component from the skin in a short time, the sheen was still significantly inhibited. In addition, in Comparative Examples 1 and 2, the gloss could be inhibited for a relatively long time. On the other hand, in the case of Comparative Example 3, after the cosmetic composition was applied to the skin, the gloss phenomenon appeared relatively quickly as time passed. This is because the straight-chain oil component added to the composition effectively inhibited the sheen phenomenon of the skin without reducing the functionality of the other ingredients in the examples.

[0157] Comparative Example 4

[0158] The mixed mushroom extract in Example 1 was used in the same amount to prepare a cosmetic composition in a state of not being manufactured in the form of liposome, and its antioxidant activity was evaluated. The results thereof are shown together with those according to Example 1 in Table 4 below.

[0159] [Table 4]

[0160] Differentiation Example 1 Comparative example 4 Radical scavenging activity (%) 57.3 54.8 ABTS radical scavenging activity (%) 51.3 49.2 Superoxide radical scavenging activity (%) 58.5 55.1

[0161] According to the above table, when the mixed mushroom extract was not captured in the form of liposome (Comparative Example 4), the overall antioxidant activity was lowered. This is considered to be because, in the liposome preparation, the mixed mushroom extract was uniformly dispersed based on the same content due to the higher absolute value of the ZETA potential, so that the antioxidant effect thereof could be maximized, while, in the case of the extract form, the antioxidant effect could not reach the desired level due to the aggregation with each other.

[0162] Simple modifications or changes of the present application all fall within the scope of the present application, and the specific scope of protection of the present application will become clear through the appended claims.

Claims

1. An emulsion-type cosmetic composition, wherein: include: (i) 0.5 to 15% by weight of a liposome containing a mixed mushroom extract, based on the powder, comprising a combination of a Chaga extract and a Ganoderma lucidum extract in a weight ratio of 1.5 to 4:1; (ii) liposomes containing 1 to 7% by weight of retinol, retinoids and / or their derivatives; (iii) a mixed hyaluronic acid comprising 3 to 8 weight percent of hyaluronic acid having a molecular weight (Mw) of 1000 to 2000 kDa and hyaluronic acid having a molecular weight (Mw) of 10 to 500 kDa combined in a weight ratio of 0.5 to 3:1; (iv) 0.5 to 70% by weight of a straight-chain oil represented by the following Formula 1; and (v)Balanced water: [Formula 1] (CH3)3Si-O-[Si(CH3)2-O] n -Si(CH3)3 In the above formula, n is 0 to 4.

2. The cosmetic composition according to claim 1, characterized in that The mixed mushroom extract further includes shiitake mushroom extract, At this time, the weight ratio of the Chaga extract to the Shiitake mushroom extract is 8 to 15:1 based on the powder.

3. The cosmetic composition according to claim 1, characterized in that The kinematic viscosity of the straight chain oil is 0.3 to 10 cSt (25° C.).

4. A method for preparing an emulsion-type cosmetic composition, characterized in that: include: a) mixing the Chaga extract and the Ganoderma lucidum extract to prepare mixed mushroom extract liposomes; b) a step of separately preparing liposomes of retinol, retinoid and / or its derivatives; and c) mixing the liposomes of the mixed mushroom extract and the liposomes of retinol, retinoid and / or its derivatives, and mixing hyaluronic acid, linear oil and water under the condition of applying shear force, The composition comprises: (i) 0.5 to 15% by weight of a liposome containing a mixed mushroom extract, based on the powder, comprising a combination of a Chaga extract and a Ganoderma lucidum extract in a weight ratio of 1.5 to 4:1; (ii) 1 to 5% by weight of liposomes containing retinol, retinoids and / or their derivatives; (iii) a mixed hyaluronic acid comprising 3 to 8 weight percent of hyaluronic acid having a molecular weight (Mw) of 1000 to 2000 kDa and hyaluronic acid having a molecular weight (Mw) of 10 to 500 kDa combined in a weight ratio of 0.5 to 3:1; (iv) 0.5 to 70% by weight of a straight-chain oil represented by the following Formula 1; and (v)Balanced water: [Formula 1] (CH3)3Si-O-[Si(CH3)2-O] n -Si(CH3)3 In the above formula, n is 0 to 4.

5. The preparation method according to claim 4, characterized in that The extraction of the Chaga extract in step a) comprises: The step of extracting Chaga powder at least once with 5 to 10 times by weight of alcohol to obtain a crude extract; a step of suspending (dispersing) a first concentrate obtained by concentrating the crude extract in 20 to 70 times by volume of water, and then extracting and concentrating with at least one organic solvent selected from the group consisting of chloroform and ethyl acetate to prepare a second concentrate; The second concentrate is dissolved in ethyl acetate, inactive ingredients are removed by adsorption through silica gel beads to elute only active ingredients, and then concentrated to obtain a third concentrate; and The third concentrated solution is added with 2 to 5 times the volume of water to suspend (disperse) the solution, and then dried and concentrated to obtain the Chaga extract.

6. The preparation method according to claim 4, characterized in that The step a) of extracting the Chaga extract comprises extracting the Chaga powder at least once using 2 to 20 times by weight of water as an extraction solvent at 10 to 80° C. for 0.5 to 50 hours to obtain the extract.

7. The preparation method according to claim 5 or 6, characterized in that: Before extracting the Chaga extract, the Chaga is crushed and roasted at 100 to 200° C. for 3 to 10 minutes.

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